Rh4BG410000

Protein VAC14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012295
Physical Location & Seq
Forward (+)
55953043 .. 55961003
7961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG410000.1

Sequence Viewer

Length: 2175 bp
ATGGCCGACGCGCTTACTGTGATTCCGGCCGGCGTGCTCCGGAACCTGGCGGACAAGCTCTATGAGAAGCGCAAGAATGCCGCTCTCGAGGTTGAGGGAATCGTGAAGCAGCTCACGACGGCTGGGGATCATGACAAGATTACGGCCGTGATCAATTTGCTCACTACGGAATTCACTTATTCACCGCAGGCGAATCACCGGAAGGGAGGGTTGATAGGTTTAGCTGCTGCAACAGTTGGGTTGACCAGCGAAGCTGCTCAGCATCTTGAGCAAATTGTGCCTCCTGTGCTTAATTCCTTTTCTGACCAAGATAGCAGAGTTCGTTATTATGCTTGTGAAGCACTATACAACATTGCAAAGGTTGTACGAGGAGATTTTATCATATTCTTTAATCAGATTTTTGATGCCTTGTGCAAGCTTTCAGCAGACTCAGATGCTAATGTACAAAGTGCTGCTCATCTTTTAGATCGGCTTGTAAAGGATATTGTTACTGAAAGCGACCAATTCAGCATTGAAGAATTTATACCATTACTGAGGGAGCGCATGAATGTTCTAAATCCCTATGTCCGCCAGTTTTTGGTAGGATGGATCACTGTTCTAGACAGCGTTCCAGATATTGATATGCTGGGTTTTCTTCCTGATTTTCTTGATGGTTTGTTTAATATGTTAAGTGATTCAAGCCATGAGATAAGACAACAGGCTGATTCAGCTCTTTCAGAGTTTCTCCAAGAGATCAAGAACTCCCCATCTGTAGATTATGGTCGCATGGCTGAAATACTGGTGCAGAGGGCTGCTTCTCCGGATGAATTTACTCGGTTAACAGCTATCACATGGATAAATGAGTTTGTAAAACTTGGTGGGGACCAGCTTGTTCCCTATTATGCTGATATATTGGGAGCAATTTTGCCCTGCATATCTGATAAAGAAGAGAAAATCAGAGTGGTTGCTCGCGAAACCAACGAAGAGCTCCGTGCCATTAAGGCTGATCCAGCTGAAGGGTTTGATGTTGGAGCAATCCTCTCTATTGCAAGGAGGCAACTATCTAGTGAATGGGAGGCTACTAGAATTGAAGCATTGCACTGGATTTCCAACCTTCTAAACAGACATCGTACTGAGGTGTTGACTTATCTGAATGACATATTTGACACCCTTTTGGAGGCACTTTCAGATCCTTCTGATAAGGTGGTTCTCCTGGTTCTAGAGGTTCATGCATGCATAGCTAAAGATCCACAACACTTCCGCCAGCTTGTTGTCTTTCTTGTTCATAATTTCCGTGTAGATAATTCTCTTCTGGAGAAGCGTGGTGCTTTGATAATTCGCCGACTCTGTGTGCTTTTAGATGCTGAAAGGGTCTACCGAGAACTGTCTACCATACTAGAAGGAGAGTCAGACCTGGATTTTGCATCTATTATGGTTCAGGCTTTGAACTTGATTTTACTTACATCCTCCGAGTTGTCTGACCTACGAGATCTTTTGAAACAATCACTTGTGAGTCCATCAGGGAAGGACTTATTTGTTTCTTTATATGCTTCATGGTGTCACTCACCCATGGCAATTATAAGCCTCTGCTTGTTATCTCAGACATATCAGCATGCAAGTACTGTGATTCAGTCTCTGGTGGAGGAAGATATTAATGTCAAATTTTTAGTCCAGCTGGATAAATTGATTCGGTTACTGGAAACTCCGATCTTTGCGTATCTTAGATTACAGCTTCTGGAACCTGGAAGGCATATGTGGCTGTTAAAAGCCCTGTATGGTCTTCTGATGTTACTTCCCCAGCAAAGTGCCGCCTTTAAAATTCTACGGACTCGTTTAAAAACTGTACCTTCGTACTCATTTAGTGGTGAGCAACCGAGGCGAACTTCTTCGGGAAATTCCTATCAGATTCTACAACACATGTCAAGTGGATCACAGGTCACTGAGGATGGTGACATAAACCAGGATAATAAGTCTTCCCATCATGGAATTAACTTCACTGCAAGACTACTGCAGTTTGAGAAAATGCAGCAGCAGCATCGCCAGCATGCGAAAGTTCAGGCACAATCAGGCATTAATTCCACATCTTCCCCAATGCAACAGGATGTGCAAAGACCTGAAGAACTCATCCGATCTTCATCAGACGTGAATAGGCCTCCTTCTAGATCTAGGAGGGGCCCAGGGCAGTTGCAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05166 ko05203 map05166 map05203
Pfam Domains
Protein Families
Vac14_Fab1_bd Vac14_Fig4_bd

Protein Analysis

724

Amino Acids

81.42

Weight (kDa)

5.54

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOR1L1_N PF24714 15 - 240 1.4e-06 TORTIFOLIA1 N-terminal
Vac14_Fab1_bd PF12755 65 - 161 8e-41 Vacuolar 14 Fab1-binding region
HEAT_EF3_N PF24987 250 - 395 9.1e-08 EF3 N-terminal HEAT repeat
Vac14_Fig4_bd PF11916 429 - 608 1.8e-72 Vacuolar protein 14 C-terminal Fig4p binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01690 AT2G01690
fragaria_vesca FvH4_4g33570 FvH4_4g33570
malus_domestica MD13G1020500.v1.1 MD16G1018900.v1.1
prunus_persica Prupe.1G333200_v2.0.a1
pyrus_communis pycom13g01730 pycom16g01700
rosa_chinensis RchiOBHm_Chr4g0443071
rosa_laevigata RLG00000005956
rosa_multiflora Rmu_sc0008069.1_g000004
rosa_roxburghii Rroxscaffold_5G00383700
rosa_rugosa Rorug04G0344200 Rorug04G0344200
rosa_samantha Rh4BG410000 Rh4CG425500 Rh4DG404900
rosa_wichuraiana Rw4G034340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1559, 2173
AccB7I CCANNNNNTGG 1 cut(s) 577
AccBSI CCGCTC 1 cut(s) 83
AccI GTMKAC 2 cut(s) 1353, 1367
AccII CGCG 2 cut(s) 11, 951
AccIII TCCGGA 2 cut(s) 39, 799
AciI CCGC 6 cut(s) 50, 81, 185, 568, 1240, 1788
AclWI GGATC 6 cut(s) 135, 596, 980, 1163, 1220, 1915
AcoI YGGCCR 3 cut(s) 3, 27, 144
AcsI RAATTY 6 cut(s) 170, 518, 806, 1640, 1797, 1873
AcuI CTGAAG 2 cut(s) 1014, 2115
AfaI GTAC 6 cut(s) 366, 444, 1111, 1600, 1824, 1832
AfiI CCNNNNNNNGG 4 cut(s) 46, 577, 995, 1156
AflIII ACRYGT 1 cut(s) 1896
AgsI TTSAA 5 cut(s) 515, 678, 1070, 1426, 1477
AjiI CACGTC 1 cut(s) 2122
AjnI CCWGG 6 cut(s) 45, 1191, 1392, 1720, 1938, 2155
Alw21I GWGCWC 2 cut(s) 39, 969
Alw26I GTCTC 1 cut(s) 1617
AlwI GGATC 6 cut(s) 135, 596, 980, 1163, 1220, 1915
AlwNI CAGNNNCTG 2 cut(s) 1615, 1714
Ama87I CYCGRG 1 cut(s) 86
Aor13HI TCCGGA 2 cut(s) 39, 799
AoxI GGCC 5 cut(s) 3, 27, 144, 2129, 2152
ApaI GGGCCC 1 cut(s) 2156
ApeKI GCWGC 9 cut(s) 109, 224, 227, 254, 452, 791, 2005, 2008, 2011
ApoI RAATTY 6 cut(s) 170, 518, 806, 1640, 1797, 1873
ArsI GACNNNNNNTTYG 4 cut(s) 1382, 1414, 1632, 1664
AseI ATTAAT 2 cut(s) 1632, 2052
Asp700I GAANNNNTTC 1 cut(s) 1864
AspLEI GCGC 3 cut(s) 13, 72, 543
AspS9I GGNCC 3 cut(s) 862, 2152, 2153
AsuHPI GGTGA 5 cut(s) 174, 188, 1536, 1856, 1940
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 1 cut(s) 862
BaeGI GKGCMC 1 cut(s) 2156
BanII GRGCYC 2 cut(s) 969, 2156
BarI GAAGNNNNNNTAC 2 cut(s) 507, 539
BbsI GAAGAC 2 cut(s) 1751, 1944
Bbv12I GWGCWC 2 cut(s) 39, 969
BbvI GCAGC 9 cut(s) 121, 211, 214, 241, 439, 778, 2017, 2020, 2023
BccI CCATC 6 cut(s) 579, 644, 754, 1504, 1919, 1965
BceAI ACGGC 3 cut(s) 131, 135, 159
BcgI CGANNNNNNTGC 2 cut(s) 1997, 2031
BciT130I CCWGG 6 cut(s) 47, 1193, 1394, 1722, 1940, 2157
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 1617
BfaI CTAG 7 cut(s) 599, 1044, 1062, 1199, 1376, 2139, 2145
BfmI CTRYAG 2 cut(s) 750, 1988
BglI GCCNNNNNGGC 1 cut(s) 980
BglII AGATCT 2 cut(s) 1468, 2141
BlpI GCTNAGC 1 cut(s) 258
BmcAI AGTACT 1 cut(s) 1600
Bme1390I CCNGG 6 cut(s) 47, 1193, 1394, 1722, 1940, 2157
Bme18I GGWCC 1 cut(s) 862
BmeT110I CYCGRG 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 2122
BmgT120I GGNCC 3 cut(s) 862, 2152, 2153
BmiI GGNNCC 5 cut(s) 44, 863, 1719, 2153, 2154
BmrFI CCNGG 6 cut(s) 47, 1193, 1394, 1722, 1940, 2157
BmsI GCATC 6 cut(s) 271, 394, 424, 1330, 1412, 2023
BpiI GAAGAC 2 cut(s) 1751, 1944
BplI GAGNNNNNCTC 2 cut(s) 1002, 1034
BpmI CTGGAG 1 cut(s) 1313
Bpu1102I GCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 287
BsaBI GATNNNNATC 1 cut(s) 2113
BsaJI CCNNGG 4 cut(s) 1548, 1853, 2155, 2156
BsaWI WCCGGW 3 cut(s) 39, 198, 799
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 577, 995, 1156
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 4 cut(s) 571, 783, 1085, 1680
Bse3DI GCAATG 2 cut(s) 351, 1073
Bse8I GATNNNNATC 1 cut(s) 2113
BseAI TCCGGA 2 cut(s) 39, 799
BseBI CCWGG 6 cut(s) 47, 1193, 1394, 1722, 1940, 2157
BseDI CCNNGG 4 cut(s) 1548, 1853, 2155, 2156
BseGI GGATG 6 cut(s) 590, 808, 1442, 1930, 2086, 2103
BseJI GATNNNNATC 1 cut(s) 2113
BseLI CCNNNNNNNGG 4 cut(s) 46, 577, 995, 1156
BseMI GCAATG 2 cut(s) 351, 1073
BseMII CTCAG 6 cut(s) 272, 444, 524, 1104, 1592, 1911
BseNI ACTGG 4 cut(s) 571, 783, 1085, 1680
BseRI GAGGAG 1 cut(s) 384
BseSI GKGCMC 1 cut(s) 2156
BseX3I CGGCCG 2 cut(s) 27, 144
BseXI GCAGC 9 cut(s) 121, 211, 214, 241, 439, 778, 2017, 2020, 2023
BseYI CCCAGC 3 cut(s) 122, 625, 1776
BsgI GTGCAG 1 cut(s) 803
Bsh1236I CGCG 2 cut(s) 11, 951
Bsh1285I CGRYCG 2 cut(s) 30, 147
BshFI GGCC 5 cut(s) 5, 29, 146, 2131, 2154
BsiEI CGRYCG 2 cut(s) 30, 147
BsiHKAI GWGCWC 2 cut(s) 39, 969
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 5 cut(s) 26, 30, 40, 199, 800
BslFI GGGAC 1 cut(s) 875
BslI CCNNNNNNNGG 4 cut(s) 46, 577, 995, 1156
BsmAI GTCTC 1 cut(s) 1617
BsmFI GGGAC 1 cut(s) 875
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 5 cut(s) 5, 29, 146, 2131, 2154
BsoBI CYCGRG 1 cut(s) 86
Bsp120I GGGCCC 1 cut(s) 2152
Bsp1286I GDGCHC 3 cut(s) 39, 969, 2156
Bsp13I TCCGGA 2 cut(s) 39, 799
Bsp1407I TGTACA 1 cut(s) 442
Bsp1720I GCTNAGC 1 cut(s) 258
Bsp19I CCATGG 1 cut(s) 1548
Bsp68I TCGCGA 1 cut(s) 951
BspACI CCGC 6 cut(s) 50, 81, 185, 568, 1240, 1788
BspANI GGCC 5 cut(s) 5, 29, 146, 2131, 2154
BspCNI CTCAG 6 cut(s) 271, 443, 525, 1105, 1591, 1912
BspEI TCCGGA 2 cut(s) 39, 799
BspFNI CGCG 2 cut(s) 11, 951
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 5 cut(s) 44, 863, 1719, 2153, 2154
BspMAI CTGCAG 1 cut(s) 1992
BspPI GGATC 6 cut(s) 135, 596, 980, 1163, 1220, 1915
BspQI GCTCTTC 1 cut(s) 957
BsrBI CCGCTC 1 cut(s) 83
BsrDI GCAATG 2 cut(s) 351, 1073
BsrFI RCCGGY 1 cut(s) 29
BsrGI TGTACA 1 cut(s) 442
BsrI ACTGG 4 cut(s) 571, 783, 1085, 1680
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 4 cut(s) 1548, 1853, 2155, 2156
BssT1I CCWWGG 1 cut(s) 1548
Bst2UI CCWGG 6 cut(s) 47, 1193, 1394, 1722, 1940, 2157
Bst4CI ACNGT 6 cut(s) 19, 235, 595, 1365, 1603, 1822
Bst6I CTCTTC 3 cut(s) 921, 957, 1293
BstAPI GCANNNNNTGC 1 cut(s) 277
BstAUI TGTACA 1 cut(s) 442
BstDEI CTNAG 7 cut(s) 258, 430, 533, 1113, 1578, 1700, 1920
BstDSI CCRYGG 1 cut(s) 1548
BstENI CCTNNNNNAGG 1 cut(s) 1154
BstF5I GGATG 6 cut(s) 590, 808, 1442, 1930, 2086, 2103
BstFNI CGCG 2 cut(s) 11, 951
BstHHI GCGC 3 cut(s) 13, 72, 543
BstMAI GTCTC 1 cut(s) 1617
BstMCI CGRYCG 2 cut(s) 30, 147
BstNI CCWGG 6 cut(s) 47, 1193, 1394, 1722, 1940, 2157
BstNSI RCATGY 4 cut(s) 1215, 1595, 1900, 2027
BstSCI CCNGG 6 cut(s) 45, 1191, 1392, 1720, 1938, 2155
BstSFI CTRYAG 2 cut(s) 750, 1988
BstSLI GKGCMC 1 cut(s) 2156
BstUI CGCG 2 cut(s) 11, 951
BstV1I GCAGC 9 cut(s) 121, 211, 214, 241, 439, 778, 2017, 2020, 2023
BstV2I GAAGAC 2 cut(s) 1751, 1944
BstX2I RGATCY 4 cut(s) 1168, 1225, 1468, 2141
BstYI RGATCY 4 cut(s) 1168, 1225, 1468, 2141
BstZI CGGCCG 2 cut(s) 27, 144
BsuRI GGCC 5 cut(s) 5, 29, 146, 2131, 2154
BtgI CCRYGG 1 cut(s) 1548
BtgZI GCGATG 1 cut(s) 2000
BtrI CACGTC 1 cut(s) 2122
BtsCI GGATG 6 cut(s) 590, 808, 1442, 1930, 2086, 2103
BtsI GCAGTG 1 cut(s) 1974
BtsIMutI CAGTG 4 cut(s) 591, 1078, 1917, 1974
BtuMI TCGCGA 1 cut(s) 951
CaiI CAGNNNCTG 2 cut(s) 1615, 1714
CciI TCATGA 1 cut(s) 130
CfoI GCGC 3 cut(s) 13, 72, 543
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 3 cut(s) 862, 2152, 2153
CseI GACGC 1 cut(s) 17
Csp6I GTAC 6 cut(s) 365, 443, 1110, 1599, 1823, 1831
CviQI GTAC 6 cut(s) 365, 443, 1110, 1599, 1823, 1831
DdeI CTNAG 7 cut(s) 258, 430, 533, 1113, 1578, 1700, 1920
DraI TTTAAA 2 cut(s) 1795, 1815
EaeI YGGCCR 3 cut(s) 3, 27, 144
EagI CGGCCG 2 cut(s) 27, 144
Eam1104I CTCTTC 3 cut(s) 921, 957, 1293
EarI CTCTTC 3 cut(s) 921, 957, 1293
EciI GGCGGA 3 cut(s) 65, 557, 1229
Ecl136II GAGCTC 1 cut(s) 967
EclXI CGGCCG 2 cut(s) 27, 144
Eco130I CCWWGG 1 cut(s) 1548
Eco147I AGGCCT 1 cut(s) 2131
Eco24I GRGCYC 2 cut(s) 969, 2156
Eco47I GGWCC 1 cut(s) 862
Eco52I CGGCCG 2 cut(s) 27, 144
Eco53kI GAGCTC 1 cut(s) 967
Eco57I CTGAAG 2 cut(s) 1014, 2115
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 967
EcoNI CCTNNNNNAGG 1 cut(s) 1154
EcoO109I RGGNCCY 1 cut(s) 2152
EcoRI GAATTC 1 cut(s) 170
EcoRII CCWGG 6 cut(s) 45, 1191, 1392, 1720, 1938, 2155
EcoT14I CCWWGG 1 cut(s) 1548
EcoT22I ATGCAT 2 cut(s) 1213, 1217
EcoT38I GRGCYC 2 cut(s) 969, 2156
ErhI CCWWGG 1 cut(s) 1548
FaqI GGGAC 1 cut(s) 875
FauNDI CATATG 1 cut(s) 1731
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 2 cut(s) 1353, 1367
FokI GGATG 6 cut(s) 597, 815, 1429, 1937, 2090, 2093
FriOI GRGCYC 2 cut(s) 969, 2156
FspBI CTAG 7 cut(s) 599, 1044, 1062, 1199, 1376, 2139, 2145
GlaI GCGC 3 cut(s) 12, 71, 542
GsaI CCCAGC 3 cut(s) 126, 629, 1780
GsuI CTGGAG 1 cut(s) 1313
HaeIII GGCC 5 cut(s) 5, 29, 146, 2131, 2154
HapII CCGG 5 cut(s) 26, 30, 40, 199, 800
HgaI GACGC 1 cut(s) 17
HhaI GCGC 3 cut(s) 13, 72, 543
Hin6I GCGC 3 cut(s) 11, 70, 541
HinP1I GCGC 3 cut(s) 11, 70, 541
HincII GTYRAC 3 cut(s) 243, 819, 1122
HindII GTYRAC 3 cut(s) 243, 819, 1122
HindIII AAGCTT 1 cut(s) 416
HpaI GTTAAC 1 cut(s) 819
HpaII CCGG 5 cut(s) 26, 30, 40, 199, 800
HphI GGTGA 5 cut(s) 174, 188, 1536, 1856, 1940
Hpy166II GTNNAC 5 cut(s) 243, 819, 1122, 1354, 1368
Hpy8I GTNNAC 5 cut(s) 243, 819, 1122, 1354, 1368
Hpy99I CGWCG 2 cut(s) 11, 121
HpyAV CCTTC 9 cut(s) 196, 989, 1103, 1182, 1373, 1498, 1719, 1836, 2145
HpyCH4III ACNGT 6 cut(s) 19, 235, 595, 1365, 1603, 1822
HpyCH4IV ACGT 1 cut(s) 2121
HpyF3I CTNAG 7 cut(s) 258, 430, 533, 1113, 1578, 1700, 1920
HpySE526I ACGT 1 cut(s) 2121
HspAI GCGC 3 cut(s) 11, 70, 541
Kpn2I TCCGGA 2 cut(s) 39, 799
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 150
KspAI GTTAAC 1 cut(s) 819
LguI GCTCTTC 1 cut(s) 957
LmnI GCTCC 5 cut(s) 42, 538, 896, 972, 1010
Lsp1109I GCAGC 9 cut(s) 121, 211, 214, 241, 439, 778, 2017, 2020, 2023
LweI GCATC 6 cut(s) 271, 394, 424, 1330, 1412, 2023
MaeI CTAG 7 cut(s) 599, 1044, 1062, 1199, 1376, 2139, 2145
MaeII ACGT 1 cut(s) 2121
MaeIII GTNAC 6 cut(s) 487, 1538, 1671, 1767, 1915, 1928
MbiI CCGCTC 1 cut(s) 83
MflI RGATCY 4 cut(s) 1168, 1225, 1468, 2141
MhlI GDGCHC 3 cut(s) 39, 969, 2156
MlyI GAGTC 5 cut(s) 422, 1317, 1394, 1501, 1801
MmeI TCCRAC 2 cut(s) 988, 1113
Mph1103I ATGCAT 2 cut(s) 1213, 1217
MroI TCCGGA 2 cut(s) 39, 799
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 1864
MspA1I CMGCKG 2 cut(s) 992, 1654
MspI CCGG 5 cut(s) 26, 30, 40, 199, 800
MspR9I CCNGG 6 cut(s) 47, 1193, 1394, 1722, 1940, 2157
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 6 cut(s) 47, 1193, 1394, 1722, 1940, 2157
MvnI CGCG 2 cut(s) 11, 951
NaeI GCCGGC 1 cut(s) 31
NcoI CCATGG 1 cut(s) 1548
NdeI CATATG 1 cut(s) 1731
NgoMIV GCCGGC 1 cut(s) 29
NlaIV GGNNCC 5 cut(s) 44, 863, 1719, 2153, 2154
NmuCI GTSAC 3 cut(s) 1538, 1915, 1928
NruI TCGCGA 1 cut(s) 951
NsiI ATGCAT 2 cut(s) 1213, 1217
NspI RCATGY 4 cut(s) 1215, 1595, 1900, 2027
PaeI GCATGC 3 cut(s) 1215, 1595, 2027
PaeR7I CTCGAG 1 cut(s) 86
PagI TCATGA 1 cut(s) 130
PasI CCCWGGG 1 cut(s) 2156
PceI AGGCCT 1 cut(s) 2131
PciI ACATGT 1 cut(s) 1896
PciSI GCTCTTC 1 cut(s) 957
PctI GAATGC 1 cut(s) 82
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 1864
PfeI GAWTC 8 cut(s) 22, 99, 193, 674, 704, 1606, 1666, 1885
PflMI CCANNNNNTGG 1 cut(s) 577
PleI GAGTC 5 cut(s) 422, 1317, 1393, 1500, 1801
PpsI GAGTC 5 cut(s) 422, 1317, 1393, 1500, 1801
PscI ACATGT 1 cut(s) 1896
PshBI ATTAAT 2 cut(s) 1632, 2052
PsiI TTATAA 2 cut(s) 1559, 2173
Psp124BI GAGCTC 1 cut(s) 969
Psp6I CCWGG 6 cut(s) 45, 1191, 1392, 1720, 1938, 2155
PspFI CCCAGC 3 cut(s) 122, 625, 1776
PspGI CCWGG 6 cut(s) 45, 1191, 1392, 1720, 1938, 2155
PspN4I GGNNCC 5 cut(s) 44, 863, 1719, 2153, 2154
PspOMI GGGCCC 1 cut(s) 2152
PspPI GGNCC 3 cut(s) 862, 2152, 2153
PstI CTGCAG 1 cut(s) 1992
PstNI CAGNNNCTG 2 cut(s) 1615, 1714
PsuI RGATCY 4 cut(s) 1168, 1225, 1468, 2141
PvuII CAGCTG 2 cut(s) 992, 1654
RruI TCGCGA 1 cut(s) 951
RsaI GTAC 6 cut(s) 366, 444, 1111, 1600, 1824, 1832
RsaNI GTAC 6 cut(s) 365, 443, 1110, 1599, 1823, 1831
SacI GAGCTC 1 cut(s) 969
SapI GCTCTTC 1 cut(s) 957
Sau96I GGNCC 3 cut(s) 862, 2152, 2153
ScaI AGTACT 1 cut(s) 1600
SchI GAGTC 5 cut(s) 422, 1317, 1394, 1501, 1801
ScrFI CCNGG 6 cut(s) 47, 1193, 1394, 1722, 1940, 2157
SduI GDGCHC 3 cut(s) 39, 969, 2156
SfaNI GCATC 6 cut(s) 271, 394, 424, 1330, 1412, 2023
SfcI CTRYAG 2 cut(s) 750, 1988
Sfr274I CTCGAG 1 cut(s) 86
SinI GGWCC 1 cut(s) 862
SlaI CTCGAG 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 86, 266
SmoI CTYRAG 2 cut(s) 86, 266
SphI GCATGC 3 cut(s) 1215, 1595, 2027
SseBI AGGCCT 1 cut(s) 2131
SsiI CCGC 6 cut(s) 50, 81, 185, 568, 1240, 1788
SspMI CTAG 7 cut(s) 599, 1044, 1062, 1199, 1376, 2139, 2145
SstI GAGCTC 1 cut(s) 969
StuI AGGCCT 1 cut(s) 2131
StyD4I CCNGG 6 cut(s) 45, 1191, 1392, 1720, 1938, 2155
StyI CCWWGG 1 cut(s) 1548
TaaI ACNGT 6 cut(s) 19, 235, 595, 1365, 1603, 1822
TaiI ACGT 1 cut(s) 2124
TaqI TCGA 1 cut(s) 87
TatI WGTACW 2 cut(s) 442, 1598
TauI GCSGC 2 cut(s) 83, 1790
TfiI GAWTC 8 cut(s) 22, 99, 193, 674, 704, 1606, 1666, 1885
TscAI CASTG 4 cut(s) 598, 1085, 1924, 1981
TseFI GTSAC 3 cut(s) 1538, 1915, 1928
TseI GCWGC 9 cut(s) 109, 224, 227, 254, 452, 791, 2005, 2008, 2011
Tsp45I GTSAC 3 cut(s) 1538, 1915, 1928
TspDTI ATGAA 6 cut(s) 560, 819, 1196, 1253, 1521, 2103
TspGWI ACGGA 4 cut(s) 182, 959, 1262, 1819
TspRI CASTG 4 cut(s) 598, 1085, 1924, 1981
Van91I CCANNNNNTGG 1 cut(s) 577
VpaK11BI GGWCC 1 cut(s) 862
VspI ATTAAT 2 cut(s) 1632, 2052
XagI CCTNNNNNAGG 1 cut(s) 1154
XapI RAATTY 6 cut(s) 170, 518, 806, 1640, 1797, 1873
XbaI TCTAGA 3 cut(s) 598, 1198, 2138
XceI RCATGY 4 cut(s) 1215, 1595, 1900, 2027
XhoI CTCGAG 1 cut(s) 86
XmiI GTMKAC 2 cut(s) 1353, 1367
XmnI GAANNNNTTC 1 cut(s) 1864
XspI CTAG 7 cut(s) 599, 1044, 1062, 1199, 1376, 2139, 2145
ZrmI AGTACT 1 cut(s) 1600
Zsp2I ATGCAT 2 cut(s) 1213, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.