Rroxscaffold_5G00383700

Protein VAC14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012295
Physical Location & Seq
Forward (+)
63313452 .. 63321341
7890 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00383700.1

Sequence Viewer

Length: 1950 bp
ATGGCCGACGCGCTTAGTGTGATTCCAGCCGGCGTGCTCCGGAACCTGGCGGACAAGCTCTATGAGAAGCGCAAGAATGCCGCTCTCGAGGTTGAGGGAATCGTGAAGCAGCTCACGACGGCTGGGGATCATGACAAGATTACGGCCGTGATCAATTTGCTCACTACGGAATTCACTTATTCACCGCAGGCGAATCACCGGAAGGGAGGGTTGATAGGTTTAGCTGCTGCAACAGTTGGGTTGACCAGCGAAGCTGCTCAGCATCTTGAGCAAATTGTGCCTCCTGTGCTTAATTCCTTTTCTGACCAAGATAGCAGAGTTCGTTATTATGCTTGTGAAGCACTATACAACATTGCAAAGGTTGTACGAGGAGATTTTATCATATTCTTTAATCAGATTTTTGATGCCTTGTGCAAGCTTTCAGCAGACTCAGATGCTAATGTACAAAGTGCTGCTCATCTTTTAGATCGACTTGTAAAGGATATTGTTACTGAAAGCGACCAATTCAGAATTTATACCATTACTGAGGAGCGCATGAATGTTCTAAATCCCTATGTCCGCCAGTTTTTGGTAGGATGGATCACTGTTCTGGACAGCGTTCCAGATATTGATATGCTGGGTTTCCTTCCTGATTTTCTTGATGGTTTGTTTAATATGTTAAGTGATTCAAGCCATGAGATAAGACAACAGGCTGATTCAGCTCTTTCAGAGTTTCTCCAAGAGATCAAGAACTCCCCATCTGTAGATTATGGTCGCATGGCTGAAATACTGGTGCAGAGGGCTGCTTCTCCGGATGAATTTACTCGGTTAACAGCTATCACATGGATAAATGAGTTTGTAAAACTTGGTGGGGACCAGCTTGTTCCCTATTATGCTGATATATTGGGAGCAATTTTGCCCTGCATATCCGATAAAGAAGAGAAAATCAGAGTGGTTGCTCGCGAAACCAATGAAGAGCTCCGTGCCATTAAGGCTGATCCAGCTGAAGGGTTTGATGTTGGAGCAATCCTCTCTATTGCAAGGAGGCAACTATCTAGTGAATGGGAGGCTACTAGAATTGAAGCATTGCACTGGATTTCCAACCTTCTAAACAGACATCGTTATGAGGTGTTGACTTATTTGAATGACATATTTGACACCCTTTTGGAGGCACTTTCAGATTCTTCTGATAAGGTGGTTCTCCTGGTTCTAGAGGCTTTGAACTTGATTTTACTTACATCCTCCGAGTTGTCTGACCTACGAGATCTTTTGAAACAATCACTTGTGAGTCCATCAGGGAAGGACTTATTTGTTTCTTTATATGCTTCATGGTGTCACTCACCCATGGCAATTATAAGCCTCTGCTTGTTATCTCAGACATATCAGCATGCAAGTACTGTGATTCAGTCTCTGGTGGAGGAAGATATTAATGTCAAATTTTTAGTCCAGCTGGATAAATTGATTCGGTTACTGGAAACTCCAATCTTTGCGTATCTTAGATTACAGCTTCTGGAAGCTGGAAGGCATATGTGGCTGTTAAAAGCCCTGTATGGTCTTCTGATGTTACTTCCCCAGCAAAGTGCCGCCTTTAAAATTCTACGGACTCGTTTAAAAACTGTACCTTCGTACTCATTTAGCGGTGAGCAACCGAGGCGAACTTCTTCAGGGAATTCCTATCAGATTCTACAACACATGTCAAGTGGATCACAGGTCACTGAGGATGGTGACATAAACCAGGACAATAAGTCTTCCCATCATGGAATTAACTTCACTGCAAGACTACAGCAGTTTGAGCAAATGCAGCAGCAGCATCGCCAGCATGCAAAAGTTCAGGCACAATCAGGCATTAATTCCACAACTTCCCCAATGCAACAGGATGTGCAAAGACCTGAAGAACTCATCCGATCTTCGTCAGACGTGAATAGGCCTCCTTCTATATCTAGGAGGGGCCCAGGGCAGTTACAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05166 ko05203 map05166 map05203
Pfam Domains
Protein Families
Vac14_Fab1_bd Vac14_Fig4_bd

Protein Analysis

649

Amino Acids

72.79

Weight (kDa)

5.37

Isoelectric Point (pI)

48.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vac14_Fab1_bd PF12755 65 - 161 6.8e-41 Vacuolar 14 Fab1-binding region
HEAT_EF3_N PF24987 229 - 392 1.2e-08 EF3 N-terminal HEAT repeat
Vac14_Fig4_bd PF11916 375 - 533 4.4e-54 Vacuolar protein 14 C-terminal Fig4p binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012295)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01690 AT2G01690
fragaria_vesca FvH4_4g33570 FvH4_4g33570
malus_domestica MD13G1020500.v1.1 MD16G1018900.v1.1
prunus_persica Prupe.1G333200_v2.0.a1
pyrus_communis pycom13g01730 pycom16g01700
rosa_chinensis RchiOBHm_Chr4g0443071
rosa_laevigata RLG00000005956
rosa_multiflora Rmu_sc0008069.1_g000004
rosa_roxburghii Rroxscaffold_5G00383700
rosa_rugosa Rorug04G0344200 Rorug04G0344200
rosa_samantha Rh4BG410000 Rh4CG425500 Rh4DG404900
rosa_wichuraiana Rw4G034340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1334, 1948
AccB7I CCANNNNNTGG 1 cut(s) 568
AccBSI CCGCTC 1 cut(s) 83
AccII CGCG 2 cut(s) 11, 942
AccIII TCCGGA 2 cut(s) 39, 790
AciI CCGC 6 cut(s) 50, 81, 185, 559, 1563, 1617
AclWI GGATC 4 cut(s) 135, 587, 971, 1690
AcoI YGGCCR 2 cut(s) 3, 144
AcsI RAATTY 6 cut(s) 170, 510, 797, 1415, 1572, 1648
AcuI CTGAAG 3 cut(s) 1005, 1626, 1890
AfaI GTAC 5 cut(s) 366, 444, 1375, 1599, 1607
AfiI CCNNNNNNNGG 4 cut(s) 46, 568, 986, 1147
AflIII ACRYGT 1 cut(s) 1671
AgsI TTSAA 5 cut(s) 669, 1061, 1123, 1201, 1252
AhdI GACNNNNNGTC 1 cut(s) 1723
AjiI CACGTC 1 cut(s) 1897
AjnI CCWGG 4 cut(s) 45, 1182, 1713, 1930
Alw21I GWGCWC 2 cut(s) 39, 960
Alw26I GTCTC 1 cut(s) 1392
AlwI GGATC 4 cut(s) 135, 587, 971, 1690
AlwNI CAGNNNCTG 2 cut(s) 1390, 1489
Ama87I CYCGRG 1 cut(s) 86
Aor13HI TCCGGA 2 cut(s) 39, 790
AoxI GGCC 4 cut(s) 3, 144, 1904, 1927
ApaI GGGCCC 1 cut(s) 1931
ApeKI GCWGC 9 cut(s) 109, 224, 227, 254, 452, 782, 1780, 1783, 1786
ApoI RAATTY 6 cut(s) 170, 510, 797, 1415, 1572, 1648
ArsI GACNNNNNNTTYG 2 cut(s) 1407, 1439
AseI ATTAAT 2 cut(s) 1407, 1827
Asp700I GAANNNNTTC 1 cut(s) 1639
AspLEI GCGC 3 cut(s) 13, 72, 534
AspS9I GGNCC 3 cut(s) 853, 1927, 1928
AsuHPI GGTGA 5 cut(s) 174, 188, 1311, 1631, 1715
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 1 cut(s) 853
BaeGI GKGCMC 1 cut(s) 1931
BanII GRGCYC 2 cut(s) 960, 1931
BbsI GAAGAC 2 cut(s) 1526, 1719
Bbv12I GWGCWC 2 cut(s) 39, 960
BbvI GCAGC 9 cut(s) 121, 211, 214, 241, 439, 769, 1792, 1795, 1798
BccI CCATC 6 cut(s) 570, 635, 745, 1279, 1694, 1740
BceAI ACGGC 3 cut(s) 131, 135, 159
BcgI CGANNNNNNTGC 2 cut(s) 1772, 1806
BciT130I CCWGG 4 cut(s) 47, 1184, 1715, 1932
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 1392
BfaI CTAG 4 cut(s) 1035, 1053, 1190, 1920
BfmI CTRYAG 2 cut(s) 741, 1760
BglI GCCNNNNNGGC 1 cut(s) 971
BglII AGATCT 1 cut(s) 1243
BlpI GCTNAGC 1 cut(s) 258
BmcAI AGTACT 1 cut(s) 1375
Bme1390I CCNGG 4 cut(s) 47, 1184, 1715, 1932
Bme18I GGWCC 1 cut(s) 853
BmeRI GACNNNNNGTC 1 cut(s) 1723
BmeT110I CYCGRG 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 1897
BmgT120I GGNCC 3 cut(s) 853, 1927, 1928
BmiI GGNNCC 4 cut(s) 44, 854, 1928, 1929
BmrFI CCNGG 4 cut(s) 47, 1184, 1715, 1932
BmsI GCATC 4 cut(s) 271, 394, 424, 1798
BpiI GAAGAC 2 cut(s) 1526, 1719
BplI GAGNNNNNCTC 2 cut(s) 993, 1025
Bpu1102I GCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 287
BsaJI CCNNGG 4 cut(s) 1323, 1628, 1930, 1931
BsaWI WCCGGW 3 cut(s) 39, 198, 790
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 568, 986, 1147
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 4 cut(s) 562, 774, 1076, 1455
Bse3DI GCAATG 2 cut(s) 351, 1064
BseAI TCCGGA 2 cut(s) 39, 790
BseBI CCWGG 4 cut(s) 47, 1184, 1715, 1932
BseDI CCNNGG 4 cut(s) 1323, 1628, 1930, 1931
BseGI GGATG 6 cut(s) 581, 799, 1217, 1705, 1861, 1878
BseLI CCNNNNNNNGG 4 cut(s) 46, 568, 986, 1147
BseMI GCAATG 2 cut(s) 351, 1064
BseMII CTCAG 5 cut(s) 272, 444, 516, 1367, 1686
BseNI ACTGG 4 cut(s) 562, 774, 1076, 1455
BseRI GAGGAG 2 cut(s) 384, 542
BseSI GKGCMC 1 cut(s) 1931
BseX3I CGGCCG 1 cut(s) 144
BseXI GCAGC 9 cut(s) 121, 211, 214, 241, 439, 769, 1792, 1795, 1798
BseYI CCCAGC 3 cut(s) 122, 616, 1551
BsgI GTGCAG 1 cut(s) 794
Bsh1236I CGCG 2 cut(s) 11, 942
Bsh1285I CGRYCG 1 cut(s) 147
BshFI GGCC 4 cut(s) 5, 146, 1906, 1929
BsiEI CGRYCG 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 39, 960
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 4 cut(s) 30, 40, 199, 791
BslFI GGGAC 1 cut(s) 866
BslI CCNNNNNNNGG 4 cut(s) 46, 568, 986, 1147
BsmAI GTCTC 1 cut(s) 1392
BsmFI GGGAC 1 cut(s) 866
BsmI GAATGC 1 cut(s) 82
BsnI GGCC 4 cut(s) 5, 146, 1906, 1929
BsoBI CYCGRG 1 cut(s) 86
Bsp120I GGGCCC 1 cut(s) 1927
Bsp1286I GDGCHC 3 cut(s) 39, 960, 1931
Bsp13I TCCGGA 2 cut(s) 39, 790
Bsp1407I TGTACA 1 cut(s) 442
Bsp143I GATC 9 cut(s) 127, 150, 466, 579, 723, 976, 1243, 1682, 1883
Bsp1720I GCTNAGC 1 cut(s) 258
Bsp19I CCATGG 1 cut(s) 1323
Bsp68I TCGCGA 1 cut(s) 942
BspACI CCGC 6 cut(s) 50, 81, 185, 559, 1563, 1617
BspANI GGCC 4 cut(s) 5, 146, 1906, 1929
BspCNI CTCAG 5 cut(s) 271, 443, 517, 1366, 1687
BspEI TCCGGA 2 cut(s) 39, 790
BspFNI CGCG 2 cut(s) 11, 942
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 4 cut(s) 44, 854, 1928, 1929
BspPI GGATC 4 cut(s) 135, 587, 971, 1690
BspQI GCTCTTC 1 cut(s) 948
BsrBI CCGCTC 1 cut(s) 83
BsrDI GCAATG 2 cut(s) 351, 1064
BsrFI RCCGGY 1 cut(s) 29
BsrGI TGTACA 1 cut(s) 442
BsrI ACTGG 4 cut(s) 562, 774, 1076, 1455
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 4 cut(s) 1323, 1628, 1930, 1931
BssMI GATC 9 cut(s) 127, 150, 466, 579, 723, 976, 1243, 1682, 1883
BssT1I CCWWGG 1 cut(s) 1323
Bst2UI CCWGG 4 cut(s) 47, 1184, 1715, 1932
Bst4CI ACNGT 4 cut(s) 235, 586, 1378, 1597
Bst6I CTCTTC 2 cut(s) 912, 948
BstAPI GCANNNNNTGC 1 cut(s) 277
BstAUI TGTACA 1 cut(s) 442
BstC8I GCNNGC 8 cut(s) 31, 35, 189, 416, 940, 1368, 1796, 1800
BstDEI CTNAG 7 cut(s) 14, 258, 430, 525, 1353, 1475, 1695
BstDSI CCRYGG 1 cut(s) 1323
BstENI CCTNNNNNAGG 1 cut(s) 1145
BstF5I GGATG 6 cut(s) 581, 799, 1217, 1705, 1861, 1878
BstFNI CGCG 2 cut(s) 11, 942
BstHHI GCGC 3 cut(s) 13, 72, 534
BstKTI GATC 9 cut(s) 130, 153, 469, 582, 726, 979, 1246, 1685, 1886
BstMAI GTCTC 1 cut(s) 1392
BstMBI GATC 9 cut(s) 127, 150, 466, 579, 723, 976, 1243, 1682, 1883
BstMCI CGRYCG 1 cut(s) 147
BstNI CCWGG 4 cut(s) 47, 1184, 1715, 1932
BstNSI RCATGY 3 cut(s) 1370, 1675, 1802
BstSCI CCNGG 4 cut(s) 45, 1182, 1713, 1930
BstSFI CTRYAG 2 cut(s) 741, 1760
BstSLI GKGCMC 1 cut(s) 1931
BstUI CGCG 2 cut(s) 11, 942
BstV1I GCAGC 9 cut(s) 121, 211, 214, 241, 439, 769, 1792, 1795, 1798
BstV2I GAAGAC 2 cut(s) 1526, 1719
BstX2I RGATCY 1 cut(s) 1243
BstYI RGATCY 1 cut(s) 1243
BstZI CGGCCG 1 cut(s) 144
BsuRI GGCC 4 cut(s) 5, 146, 1906, 1929
BtgI CCRYGG 1 cut(s) 1323
BtgZI GCGATG 1 cut(s) 1775
BtrI CACGTC 1 cut(s) 1897
BtsCI GGATG 6 cut(s) 581, 799, 1217, 1705, 1861, 1878
BtsI GCAGTG 1 cut(s) 1749
BtsIMutI CAGTG 4 cut(s) 582, 1069, 1692, 1749
BtuMI TCGCGA 1 cut(s) 942
Cac8I GCNNGC 8 cut(s) 31, 35, 189, 416, 940, 1368, 1796, 1800
CaiI CAGNNNCTG 2 cut(s) 1390, 1489
CciI TCATGA 1 cut(s) 130
CfoI GCGC 3 cut(s) 13, 72, 534
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 3 cut(s) 853, 1927, 1928
CseI GACGC 1 cut(s) 17
Csp6I GTAC 5 cut(s) 365, 443, 1374, 1598, 1606
CviQI GTAC 5 cut(s) 365, 443, 1374, 1598, 1606
DdeI CTNAG 7 cut(s) 14, 258, 430, 525, 1353, 1475, 1695
DpnI GATC 9 cut(s) 129, 152, 468, 581, 725, 978, 1245, 1684, 1885
DpnII GATC 9 cut(s) 127, 150, 466, 579, 723, 976, 1243, 1682, 1883
DraI TTTAAA 2 cut(s) 1570, 1590
DriI GACNNNNNGTC 1 cut(s) 1723
EaeI YGGCCR 2 cut(s) 3, 144
EagI CGGCCG 1 cut(s) 144
Eam1104I CTCTTC 2 cut(s) 912, 948
Eam1105I GACNNNNNGTC 1 cut(s) 1723
EarI CTCTTC 2 cut(s) 912, 948
EciI GGCGGA 2 cut(s) 65, 548
Ecl136II GAGCTC 1 cut(s) 958
EclXI CGGCCG 1 cut(s) 144
Eco130I CCWWGG 1 cut(s) 1323
Eco147I AGGCCT 1 cut(s) 1906
Eco24I GRGCYC 2 cut(s) 960, 1931
Eco47I GGWCC 1 cut(s) 853
Eco52I CGGCCG 1 cut(s) 144
Eco53kI GAGCTC 1 cut(s) 958
Eco57I CTGAAG 3 cut(s) 1005, 1626, 1890
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 958
EcoNI CCTNNNNNAGG 1 cut(s) 1145
EcoO109I RGGNCCY 1 cut(s) 1927
EcoRI GAATTC 2 cut(s) 170, 1648
EcoRII CCWGG 4 cut(s) 45, 1182, 1713, 1930
EcoT14I CCWWGG 1 cut(s) 1323
EcoT38I GRGCYC 2 cut(s) 960, 1931
ErhI CCWWGG 1 cut(s) 1323
FaqI GGGAC 1 cut(s) 866
FauNDI CATATG 1 cut(s) 1506
FbaI TGATCA 1 cut(s) 150
FokI GGATG 6 cut(s) 588, 806, 1204, 1712, 1865, 1868
FriOI GRGCYC 2 cut(s) 960, 1931
FspBI CTAG 4 cut(s) 1035, 1053, 1190, 1920
GlaI GCGC 3 cut(s) 12, 71, 533
GsaI CCCAGC 3 cut(s) 126, 620, 1555
HaeIII GGCC 4 cut(s) 5, 146, 1906, 1929
HapII CCGG 4 cut(s) 30, 40, 199, 791
HgaI GACGC 1 cut(s) 17
HhaI GCGC 3 cut(s) 13, 72, 534
Hin6I GCGC 3 cut(s) 11, 70, 532
HinP1I GCGC 3 cut(s) 11, 70, 532
HincII GTYRAC 3 cut(s) 243, 810, 1113
HindII GTYRAC 3 cut(s) 243, 810, 1113
HindIII AAGCTT 1 cut(s) 416
HpaI GTTAAC 1 cut(s) 810
HpaII CCGG 4 cut(s) 30, 40, 199, 791
HphI GGTGA 5 cut(s) 174, 188, 1311, 1631, 1715
Hpy166II GTNNAC 3 cut(s) 243, 810, 1113
Hpy8I GTNNAC 3 cut(s) 243, 810, 1113
Hpy99I CGWCG 2 cut(s) 11, 121
HpyAV CCTTC 8 cut(s) 196, 635, 980, 1094, 1273, 1494, 1611, 1920
HpyCH4III ACNGT 4 cut(s) 235, 586, 1378, 1597
HpyCH4IV ACGT 1 cut(s) 1896
HpyF3I CTNAG 7 cut(s) 14, 258, 430, 525, 1353, 1475, 1695
HpySE526I ACGT 1 cut(s) 1896
HspAI GCGC 3 cut(s) 11, 70, 532
Kpn2I TCCGGA 2 cut(s) 39, 790
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 150
KspAI GTTAAC 1 cut(s) 810
Kzo9I GATC 9 cut(s) 127, 150, 466, 579, 723, 976, 1243, 1682, 1883
LguI GCTCTTC 1 cut(s) 948
LmnI GCTCC 5 cut(s) 42, 529, 887, 963, 1001
Lsp1109I GCAGC 9 cut(s) 121, 211, 214, 241, 439, 769, 1792, 1795, 1798
LweI GCATC 4 cut(s) 271, 394, 424, 1798
MaeI CTAG 4 cut(s) 1035, 1053, 1190, 1920
MaeII ACGT 1 cut(s) 1896
MaeIII GTNAC 7 cut(s) 487, 1313, 1446, 1542, 1690, 1703, 1938
MalI GATC 9 cut(s) 129, 152, 468, 581, 725, 978, 1245, 1684, 1885
MbiI CCGCTC 1 cut(s) 83
MboI GATC 9 cut(s) 127, 150, 466, 579, 723, 976, 1243, 1682, 1883
MboII GAAGA 9 cut(s) 929, 965, 1155, 1412, 1526, 1632, 1719, 1878, 1883
MflI RGATCY 1 cut(s) 1243
MhlI GDGCHC 3 cut(s) 39, 960, 1931
MlyI GAGTC 3 cut(s) 422, 1276, 1576
MmeI TCCRAC 2 cut(s) 979, 1104
MroI TCCGGA 2 cut(s) 39, 790
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 1639
MslI CAYNNNNRTG 1 cut(s) 1101
MspA1I CMGCKG 2 cut(s) 983, 1429
MspI CCGG 4 cut(s) 30, 40, 199, 791
MspR9I CCNGG 4 cut(s) 47, 1184, 1715, 1932
Mva1269I GAATGC 1 cut(s) 82
MvaI CCWGG 4 cut(s) 47, 1184, 1715, 1932
MvnI CGCG 2 cut(s) 11, 942
NaeI GCCGGC 1 cut(s) 31
NcoI CCATGG 1 cut(s) 1323
NdeI CATATG 1 cut(s) 1506
NdeII GATC 9 cut(s) 127, 150, 466, 579, 723, 976, 1243, 1682, 1883
NgoMIV GCCGGC 1 cut(s) 29
NlaIV GGNNCC 4 cut(s) 44, 854, 1928, 1929
NmuCI GTSAC 3 cut(s) 1313, 1690, 1703
NruI TCGCGA 1 cut(s) 942
NspI RCATGY 3 cut(s) 1370, 1675, 1802
PaeI GCATGC 2 cut(s) 1370, 1802
PaeR7I CTCGAG 1 cut(s) 86
PagI TCATGA 1 cut(s) 130
PasI CCCWGGG 1 cut(s) 1931
PceI AGGCCT 1 cut(s) 1906
PciI ACATGT 1 cut(s) 1671
PciSI GCTCTTC 1 cut(s) 948
PctI GAATGC 1 cut(s) 82
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 1639
PfeI GAWTC 9 cut(s) 22, 99, 193, 665, 695, 1160, 1381, 1441, 1660
PflMI CCANNNNNTGG 1 cut(s) 568
PleI GAGTC 3 cut(s) 422, 1275, 1576
PpsI GAGTC 3 cut(s) 422, 1275, 1576
PscI ACATGT 1 cut(s) 1671
PshBI ATTAAT 2 cut(s) 1407, 1827
PsiI TTATAA 2 cut(s) 1334, 1948
Psp124BI GAGCTC 1 cut(s) 960
Psp6I CCWGG 4 cut(s) 45, 1182, 1713, 1930
PspFI CCCAGC 3 cut(s) 122, 616, 1551
PspGI CCWGG 4 cut(s) 45, 1182, 1713, 1930
PspN4I GGNNCC 4 cut(s) 44, 854, 1928, 1929
PspOMI GGGCCC 1 cut(s) 1927
PspPI GGNCC 3 cut(s) 853, 1927, 1928
PstNI CAGNNNCTG 2 cut(s) 1390, 1489
PsuI RGATCY 1 cut(s) 1243
PvuII CAGCTG 2 cut(s) 983, 1429
RruI TCGCGA 1 cut(s) 942
RsaI GTAC 5 cut(s) 366, 444, 1375, 1599, 1607
RsaNI GTAC 5 cut(s) 365, 443, 1374, 1598, 1606
RseI CAYNNNNRTG 1 cut(s) 1101
SacI GAGCTC 1 cut(s) 960
SapI GCTCTTC 1 cut(s) 948
Sau3AI GATC 9 cut(s) 127, 150, 466, 579, 723, 976, 1243, 1682, 1883
Sau96I GGNCC 3 cut(s) 853, 1927, 1928
ScaI AGTACT 1 cut(s) 1375
SchI GAGTC 3 cut(s) 422, 1276, 1576
ScrFI CCNGG 4 cut(s) 47, 1184, 1715, 1932
SduI GDGCHC 3 cut(s) 39, 960, 1931
SfaNI GCATC 4 cut(s) 271, 394, 424, 1798
SfcI CTRYAG 2 cut(s) 741, 1760
Sfr274I CTCGAG 1 cut(s) 86
SinI GGWCC 1 cut(s) 853
SlaI CTCGAG 1 cut(s) 86
SmiMI CAYNNNNRTG 1 cut(s) 1101
SmlI CTYRAG 2 cut(s) 86, 266
SmoI CTYRAG 2 cut(s) 86, 266
SphI GCATGC 2 cut(s) 1370, 1802
SseBI AGGCCT 1 cut(s) 1906
SsiI CCGC 6 cut(s) 50, 81, 185, 559, 1563, 1617
SspMI CTAG 4 cut(s) 1035, 1053, 1190, 1920
SstI GAGCTC 1 cut(s) 960
StuI AGGCCT 1 cut(s) 1906
StyD4I CCNGG 4 cut(s) 45, 1182, 1713, 1930
StyI CCWWGG 1 cut(s) 1323
TaaI ACNGT 4 cut(s) 235, 586, 1378, 1597
TaiI ACGT 1 cut(s) 1899
TaqI TCGA 2 cut(s) 87, 469
TatI WGTACW 2 cut(s) 442, 1373
TauI GCSGC 2 cut(s) 83, 1565
TfiI GAWTC 9 cut(s) 22, 99, 193, 665, 695, 1160, 1381, 1441, 1660
TscAI CASTG 4 cut(s) 589, 1076, 1699, 1756
TseFI GTSAC 3 cut(s) 1313, 1690, 1703
TseI GCWGC 9 cut(s) 109, 224, 227, 254, 452, 782, 1780, 1783, 1786
Tsp45I GTSAC 3 cut(s) 1313, 1690, 1703
TspDTI ATGAA 4 cut(s) 551, 810, 966, 1296
TspGWI ACGGA 3 cut(s) 182, 950, 1594
TspRI CASTG 4 cut(s) 589, 1076, 1699, 1756
Van91I CCANNNNNTGG 1 cut(s) 568
VpaK11BI GGWCC 1 cut(s) 853
VspI ATTAAT 2 cut(s) 1407, 1827
XagI CCTNNNNNAGG 1 cut(s) 1145
XapI RAATTY 6 cut(s) 170, 510, 797, 1415, 1572, 1648
XbaI TCTAGA 1 cut(s) 1189
XceI RCATGY 3 cut(s) 1370, 1675, 1802
XhoI CTCGAG 1 cut(s) 86
XmnI GAANNNNTTC 1 cut(s) 1639
XspI CTAG 4 cut(s) 1035, 1053, 1190, 1920
ZrmI AGTACT 1 cut(s) 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.