FvH4_4g33700

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018032
Physical Location & Seq
Reverse (-)
31830777 .. 31834000
3224 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33700.t1

Sequence Viewer

Length: 1803 bp
ATGGCAGCCAAAGGTGACACGAGCTCATCCAAAGTGCGCGGTAGAGAATTTCTCCTGGAGATTGAGGCAAAAGTTCGAAAATGGTGGGAAGACGAGAAAGTTTTCAGTGCTGAATGTGAAAAGCCTGGTCACAAGTTCTTTGGAAAATTCCCTTTTGATAGTTTTATGGGAAATCCCTTCAGTCTATCAAAGCTGGAATGGCTGCCAGATATTCCTATTGATGTCATGAGATTTGCTTTGGTATATGTTTGTGATGGTGCTGTTGATGAGGCAAAAATCAACTCTCTACATAGCAATGCAGCTTCTGTATACAATGCTGCAAACACTGCCATCCTAAAATTCAATGAAGAGATGTCATGGCATGAAGAATTCTTGGCTGCAGATTTTGATTCTTATTTGAGATGTGGTCCACCTTCTACTTTCGCAGACAGGGTGTTTGCCAATGAGATAAACATTGCCTTAAACAGGACCAAAGAGCAATACGAAGCTGGCCAGTTTCGAGGAGCCCTGGATTCCATTTTTGAGCTTCAAGATGCCAGAGATTGGTACAAGATTTCATGTGGTGGTACAAATGCAATGAACCGTGACTTGGTGTGGCGTTTTATGGATGTGCAGACATGTCTTATAACTCCAATCTGTCCACACTATGCGGAGTATGTCTGGAGAGATCTCCTGCACAAGATCGACTTTGTGGTGAATGCAGACTGGCCTGTCGCTGATGCTCCAGATGAGTCGATCCTTCAGAGTGCCGATAAGTATTTGAAAGACTTAATTGATTCAATGAAGAGACAGGTTGAAAGTGAGAGAAATAAGAAGGGTACTGCTCTAGCTACCACTAGTTTGACAGAAGAGAGACAGAAATTAGAAGCTCTTATATATGTTAATGAGGCAGGTGATACACATACTCTTGCTACAAAAGGTTTGCATACAGATTCTAAACAAAACGGAAATGAGTTCAAGCAGACCGCGAAAGTTAAATTCGGGGATCAGTACTTATATTTGAAGCTGCCTTTTAGGGAGAAGGTGCTTCTACAGAATGTGGACTTTATTAAGAGGGAAATTGGTCTTGACAAGGTGCAAGTTTTATCAGCTACCAACCCTTGTGATCTTGCTAAAGCTGGTTCCCCTGTTAAAATGTCAGATGCTGAATCTTCCTCACGTGGAAATCCAGCTGCTCTCTCCATGACATGTCCAGAAAAAGATTTTAGACTGTTTGGTGCTTCAAAGACTACACCATTACTGGCTGATCTATCCTCATCTGGCTTTGCACCAAAGCACACCAACCCAAAACGGGATCTAGTTCCGTTGTTCAGTACTAGCCCCGAGAGTACTACATTAACTAAAGAGGAAGAAGAAGAGGAACGGCTGCGCGCATTGGAAGCTTTTGTGGAGAGGATGAAGTTGGAGACGAGTAATGCAAGTACCTCCAAAGGAAATGCAAGAGAAGAAGAGGAACTGCGGCGCGCAATGGCGGCTTCCATGGAGTCGGGAAGTGGAAGTACCTCCATAAAGGATCCACTAGAGATGTTCAATTTCGAACCGACAGTTATTCATGTAACTGAAGTTGATGAGGAGGTATCACCACTGTGGTCGATGGCAGATTTATTAGAAGAAAGTAGTGCAAGTAGCTCTAAAGTCCAGAATATTGTAACTAGAGAAGAAGAAGATGATGAGGAACTGCAGCGCGAATTGGTGGCTTCCTTGGGGAGCTGGTGGCCAGGTAGTGCAAGTACCAAAGAAGAAGAAGAAGAAGAAGATAAGGAACTGCAACGCGCATTGGCAGCGTCCCTGGAAAATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

601

Amino Acids

67.44

Weight (kDa)

4.82

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anticodon_1 PF08264 143 - 274 1.5e-12 Anticodon-binding domain of tRNA ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018032)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33690 FvH4_4g33700
rosa_chinensis RchiOBHm_Chr4g0443171
rosa_laevigata RLG00000005950
rosa_multiflora Rmu_sc0007968.1_g000006
rosa_roxburghii Rroxscaffold_5G00383770
rosa_rugosa Rorug04G0344700
rosa_samantha Rh4BG410800 Rh4CG426300 Rh4DG405900
rosa_wichuraiana Rw4G034420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 626
AarI CACCTGC 1 cut(s) 881
AasI GACNNNNNNGTC 1 cut(s) 710
Acc36I ACCTGC 1 cut(s) 881
AccB7I CCANNNNNTGG 1 cut(s) 543
AccI GTMKAC 1 cut(s) 309
AccII CGCG 6 cut(s) 39, 968, 1371, 1464, 1686, 1773
AciI CCGC 5 cut(s) 39, 650, 966, 1459, 1472
AclWI GGATC 5 cut(s) 730, 993, 1302, 1508, 1521
AcoI YGGCCR 2 cut(s) 490, 1715
AcsI RAATTY 5 cut(s) 47, 146, 338, 368, 977
AcuI CTGAAG 3 cut(s) 163, 725, 1581
AcvI CACGTG 1 cut(s) 1160
AfaI GTAC 9 cut(s) 548, 568, 820, 992, 1315, 1330, 1423, 1501, 1732
AfiI CCNNNNNNNGG 4 cut(s) 465, 543, 589, 1291
AflIII ACRYGT 2 cut(s) 617, 1187
AgsI TTSAA 9 cut(s) 343, 530, 763, 780, 797, 958, 1003, 1224, 1531
AhlI ACTAGT 1 cut(s) 836
AjnI CCWGG 5 cut(s) 54, 124, 507, 1717, 1788
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AleI CACNNNNGTG 1 cut(s) 1585
Alw21I GWGCWC 1 cut(s) 26
Alw26I GTCTC 3 cut(s) 781, 847, 1400
AlwI GGATC 5 cut(s) 730, 993, 1302, 1508, 1521
AlwNI CAGNNNCTG 2 cut(s) 305, 1145
Ama87I CYCGRG 1 cut(s) 1322
AoxI GGCC 3 cut(s) 490, 707, 1715
ApoI RAATTY 5 cut(s) 47, 146, 338, 368, 977
ArsI GACNNNNNNTTYG 2 cut(s) 419, 451
Asp700I GAANNNNTTC 1 cut(s) 101
AspLEI GCGC 7 cut(s) 39, 1371, 1373, 1464, 1466, 1686, 1775
AspS9I GGNCC 2 cut(s) 407, 468
AsuHPI GGTGA 4 cut(s) 26, 706, 905, 1572
AsuII TTCGAA 2 cut(s) 76, 1536
AvaI CYCGRG 1 cut(s) 1322
AvaII GGWCC 2 cut(s) 407, 468
BalI TGGCCA 2 cut(s) 492, 1717
BamHI GGATCC 1 cut(s) 1513
BanII GRGCYC 2 cut(s) 26, 508
BarI GAAGNNNNNNTAC 2 cut(s) 1483, 1515
BauI CACGAG 1 cut(s) 19
BbrPI CACGTG 1 cut(s) 1160
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 26
BccI CCATC 3 cut(s) 248, 338, 1588
BceAI ACGGC 1 cut(s) 1379
BciT130I CCWGG 5 cut(s) 56, 126, 509, 1719, 1790
BcoDI GTCTC 3 cut(s) 781, 847, 1400
BcuI ACTAGT 1 cut(s) 836
BfaI CTAG 6 cut(s) 827, 837, 1298, 1317, 1520, 1653
BfmI CTRYAG 3 cut(s) 378, 1031, 1679
BfuAI ACCTGC 1 cut(s) 881
BglII AGATCT 1 cut(s) 667
BmcAI AGTACT 3 cut(s) 992, 1315, 1330
Bme1390I CCNGG 5 cut(s) 56, 126, 509, 1719, 1790
Bme18I GGWCC 2 cut(s) 407, 468
BmeT110I CYCGRG 1 cut(s) 1322
BmgT120I GGNCC 2 cut(s) 407, 468
BmiI GGNNCC 3 cut(s) 505, 1123, 1515
BmrFI CCNGG 5 cut(s) 56, 126, 509, 1719, 1790
BmsI GCATC 3 cut(s) 523, 709, 1132
BpiI GAAGAC 1 cut(s) 96
BplI GAGNNNNNCTC 2 cut(s) 36, 68
BpmI CTGGAG 3 cut(s) 77, 682, 708
Bpu14I TTCGAA 2 cut(s) 76, 1536
BsaAI YACGTR 1 cut(s) 1160
BsaJI CCNNGG 4 cut(s) 507, 1479, 1701, 1788
Bsc4I CCNNNNNNNGG 4 cut(s) 465, 543, 589, 1291
Bse1I ACTGG 3 cut(s) 493, 710, 1245
Bse3DI GCAATG 4 cut(s) 301, 453, 582, 1473
BseBI CCWGG 5 cut(s) 56, 126, 509, 1719, 1790
BseDI CCNNGG 4 cut(s) 507, 1479, 1701, 1788
BseGI GGATG 4 cut(s) 26, 330, 613, 1401
BseLI CCNNNNNNNGG 4 cut(s) 465, 543, 589, 1291
BseMI GCAATG 4 cut(s) 301, 453, 582, 1473
BseNI ACTGG 3 cut(s) 493, 710, 1245
BsePI GCGCGC 2 cut(s) 1369, 1462
BseRI GAGGAG 2 cut(s) 516, 1586
BsgI GTGCAG 2 cut(s) 632, 659
Bsh1236I CGCG 6 cut(s) 39, 968, 1371, 1464, 1686, 1773
BshFI GGCC 3 cut(s) 492, 709, 1717
BsiHKAI GWGCWC 1 cut(s) 26
BsiHKCI CYCGRG 1 cut(s) 1322
BslFI GGGAC 1 cut(s) 1771
BslI CCNNNNNNNGG 4 cut(s) 465, 543, 589, 1291
BsmAI GTCTC 3 cut(s) 781, 847, 1400
BsmBI CGTCTC 1 cut(s) 1400
BsmFI GGGAC 1 cut(s) 1771
BsmI GAATGC 1 cut(s) 703
BsnI GGCC 3 cut(s) 492, 709, 1717
BsoBI CYCGRG 1 cut(s) 1322
Bsp119I TTCGAA 2 cut(s) 76, 1536
Bsp1286I GDGCHC 2 cut(s) 26, 508
Bsp143I GATC 8 cut(s) 667, 681, 735, 985, 1105, 1246, 1294, 1513
Bsp19I CCATGG 1 cut(s) 1479
BspACI CCGC 5 cut(s) 39, 650, 966, 1459, 1472
BspANI GGCC 3 cut(s) 492, 709, 1717
BspFNI CGCG 6 cut(s) 39, 968, 1371, 1464, 1686, 1773
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 3 cut(s) 505, 1123, 1515
BspMAI CTGCAG 2 cut(s) 382, 1683
BspMI ACCTGC 1 cut(s) 881
BspPI GGATC 5 cut(s) 730, 993, 1302, 1508, 1521
BspT104I TTCGAA 2 cut(s) 76, 1536
BsrDI GCAATG 4 cut(s) 301, 453, 582, 1473
BsrI ACTGG 3 cut(s) 493, 710, 1245
BssECI CCNNGG 4 cut(s) 507, 1479, 1701, 1788
BssHII GCGCGC 2 cut(s) 1369, 1462
BssMI GATC 8 cut(s) 667, 681, 735, 985, 1105, 1246, 1294, 1513
BssNAI GTATAC 1 cut(s) 310
BssSI CACGAG 1 cut(s) 19
BssT1I CCWWGG 2 cut(s) 1479, 1701
Bst1107I GTATAC 1 cut(s) 310
Bst2BI CACGAG 1 cut(s) 19
Bst2UI CCWGG 5 cut(s) 56, 126, 509, 1719, 1790
Bst4CI ACNGT 4 cut(s) 584, 1212, 1546, 1587
Bst6I CTCTTC 5 cut(s) 342, 779, 843, 1350, 1443
BstAPI GCANNNNNTGC 1 cut(s) 326
BstBAI YACGTR 1 cut(s) 1160
BstBI TTCGAA 2 cut(s) 76, 1536
BstC8I GCNNGC 3 cut(s) 490, 1371, 1464
BstDSI CCRYGG 1 cut(s) 1479
BstENI CCTNNNNNAGG 1 cut(s) 463
BstF5I GGATG 4 cut(s) 26, 330, 613, 1401
BstFNI CGCG 6 cut(s) 39, 968, 1371, 1464, 1686, 1773
BstHHI GCGC 7 cut(s) 39, 1371, 1373, 1464, 1466, 1686, 1775
BstKTI GATC 8 cut(s) 670, 684, 738, 988, 1108, 1249, 1297, 1516
BstMAI GTCTC 3 cut(s) 781, 847, 1400
BstMBI GATC 8 cut(s) 667, 681, 735, 985, 1105, 1246, 1294, 1513
BstMWI GCNNNNNNNGC 5 cut(s) 199, 326, 1379, 1472, 1781
BstNI CCWGG 5 cut(s) 56, 126, 509, 1719, 1790
BstNSI RCATGY 2 cut(s) 621, 1191
BstSCI CCNGG 5 cut(s) 54, 124, 507, 1717, 1788
BstSFI CTRYAG 3 cut(s) 378, 1031, 1679
BstUI CGCG 6 cut(s) 39, 968, 1371, 1464, 1686, 1773
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 3 cut(s) 667, 1294, 1513
BstYI RGATCY 3 cut(s) 667, 1294, 1513
BstZ17I GTATAC 1 cut(s) 310
BsuRI GGCC 3 cut(s) 492, 709, 1717
BtgI CCRYGG 1 cut(s) 1479
BtsCI GGATG 4 cut(s) 26, 330, 613, 1401
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 3 cut(s) 112, 324, 1583
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 3 cut(s) 490, 1371, 1464
CaiI CAGNNNCTG 2 cut(s) 305, 1145
CciI TCATGA 1 cut(s) 225
CfoI GCGC 7 cut(s) 39, 1371, 1373, 1464, 1466, 1686, 1775
Cfr13I GGNCC 2 cut(s) 407, 468
CseI GACGC 1 cut(s) 1773
Csp6I GTAC 9 cut(s) 547, 567, 819, 991, 1314, 1329, 1422, 1500, 1731
CviAII CATG 9 cut(s) 226, 357, 362, 558, 618, 1183, 1188, 1480, 1553
CviQI GTAC 9 cut(s) 547, 567, 819, 991, 1314, 1329, 1422, 1500, 1731
DpnI GATC 8 cut(s) 669, 683, 737, 987, 1107, 1248, 1296, 1515
DpnII GATC 8 cut(s) 667, 681, 735, 985, 1105, 1246, 1294, 1513
DrdI GACNNNNNNGTC 1 cut(s) 710
DseDI GACNNNNNNGTC 1 cut(s) 710
EaeI YGGCCR 2 cut(s) 490, 1715
Eam1104I CTCTTC 5 cut(s) 342, 779, 843, 1350, 1443
EarI CTCTTC 5 cut(s) 342, 779, 843, 1350, 1443
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 2 cut(s) 1479, 1701
Eco24I GRGCYC 2 cut(s) 26, 508
Eco47I GGWCC 2 cut(s) 407, 468
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 3 cut(s) 163, 725, 1581
Eco72I CACGTG 1 cut(s) 1160
Eco88I CYCGRG 1 cut(s) 1322
EcoICRI GAGCTC 1 cut(s) 24
EcoNI CCTNNNNNAGG 1 cut(s) 463
EcoRI GAATTC 1 cut(s) 368
EcoRII CCWGG 5 cut(s) 54, 124, 507, 1717, 1788
EcoT14I CCWWGG 2 cut(s) 1479, 1701
EcoT38I GRGCYC 2 cut(s) 26, 508
ErhI CCWWGG 2 cut(s) 1479, 1701
Esp3I CGTCTC 1 cut(s) 1400
FaeI CATG 9 cut(s) 229, 360, 365, 561, 621, 1186, 1191, 1483, 1556
FalI AAGNNNNNCTT 2 cut(s) 671, 703
FaqI GGGAC 1 cut(s) 1771
FatI CATG 9 cut(s) 225, 356, 361, 557, 617, 1182, 1187, 1479, 1552
FblI GTMKAC 1 cut(s) 309
FokI GGATG 4 cut(s) 13, 317, 620, 1408
FriOI GRGCYC 2 cut(s) 26, 508
FspBI CTAG 6 cut(s) 827, 837, 1298, 1317, 1520, 1653
GlaI GCGC 7 cut(s) 38, 1370, 1372, 1463, 1465, 1685, 1774
GsuI CTGGAG 3 cut(s) 77, 682, 708
HaeIII GGCC 3 cut(s) 492, 709, 1717
HgaI GACGC 1 cut(s) 1773
HhaI GCGC 7 cut(s) 39, 1371, 1373, 1464, 1466, 1686, 1775
Hin1II CATG 9 cut(s) 229, 360, 365, 561, 621, 1186, 1191, 1483, 1556
Hin6I GCGC 7 cut(s) 37, 1369, 1371, 1462, 1464, 1684, 1773
HinP1I GCGC 7 cut(s) 37, 1369, 1371, 1462, 1464, 1684, 1773
HindIII AAGCTT 1 cut(s) 1380
HinfI GANTC 7 cut(s) 389, 512, 731, 776, 932, 1148, 1484
HphI GGTGA 4 cut(s) 26, 706, 905, 1572
Hpy166II GTNNAC 4 cut(s) 310, 410, 641, 1042
Hpy188I TCNGA 2 cut(s) 744, 1141
Hpy188III TCNNGA 8 cut(s) 226, 530, 661, 725, 1067, 1193, 1488, 1639
Hpy8I GTNNAC 4 cut(s) 310, 410, 641, 1042
HpyAV CCTTC 5 cut(s) 187, 423, 749, 808, 1015
HpyCH4III ACNGT 4 cut(s) 584, 1212, 1546, 1587
HpyCH4IV ACGT 1 cut(s) 1159
HpyF10VI GCNNNNNNNGC 5 cut(s) 199, 326, 1379, 1472, 1781
HpySE526I ACGT 1 cut(s) 1159
Hsp92II CATG 9 cut(s) 229, 360, 365, 561, 621, 1186, 1191, 1483, 1556
HspAI GCGC 7 cut(s) 37, 1369, 1371, 1462, 1464, 1684, 1773
Kzo9I GATC 8 cut(s) 667, 681, 735, 985, 1105, 1246, 1294, 1513
LmnI GCTCC 3 cut(s) 503, 727, 1707
LweI GCATC 3 cut(s) 523, 709, 1132
MaeI CTAG 6 cut(s) 827, 837, 1298, 1317, 1520, 1653
MaeII ACGT 1 cut(s) 1159
MaeIII GTNAC 5 cut(s) 14, 128, 584, 1555, 1648
MalI GATC 8 cut(s) 669, 683, 737, 987, 1107, 1248, 1296, 1515
MboI GATC 8 cut(s) 667, 681, 735, 985, 1105, 1246, 1294, 1513
MflI RGATCY 3 cut(s) 667, 1294, 1513
MhlI GDGCHC 2 cut(s) 26, 508
MlsI TGGCCA 2 cut(s) 492, 1717
MluNI TGGCCA 2 cut(s) 492, 1717
MlyI GAGTC 2 cut(s) 740, 1493
MmeI TCCRAC 1 cut(s) 1383
Mox20I TGGCCA 2 cut(s) 492, 1717
MroXI GAANNNNTTC 1 cut(s) 101
MscI TGGCCA 2 cut(s) 492, 1717
MseI TTAA 7 cut(s) 461, 770, 882, 975, 1050, 1131, 1337
MslI CAYNNNNRTG 2 cut(s) 294, 1585
Msp20I TGGCCA 2 cut(s) 492, 1717
MspA1I CMGCKG 1 cut(s) 1172
MspR9I CCNGG 5 cut(s) 56, 126, 509, 1719, 1790
Mva1269I GAATGC 1 cut(s) 703
MvaI CCWGG 5 cut(s) 56, 126, 509, 1719, 1790
MvnI CGCG 6 cut(s) 39, 968, 1371, 1464, 1686, 1773
MwoI GCNNNNNNNGC 5 cut(s) 199, 326, 1379, 1472, 1781
NcoI CCATGG 1 cut(s) 1479
NdeII GATC 8 cut(s) 667, 681, 735, 985, 1105, 1246, 1294, 1513
NlaIII CATG 9 cut(s) 229, 360, 365, 561, 621, 1186, 1191, 1483, 1556
NlaIV GGNNCC 3 cut(s) 505, 1123, 1515
NmuCI GTSAC 3 cut(s) 14, 128, 584
NspI RCATGY 2 cut(s) 621, 1191
NspV TTCGAA 2 cut(s) 76, 1536
OliI CACNNNNGTG 1 cut(s) 1585
PagI TCATGA 1 cut(s) 225
PaqCI CACCTGC 1 cut(s) 881
PauI GCGCGC 2 cut(s) 1369, 1462
PciI ACATGT 2 cut(s) 617, 1187
PctI GAATGC 1 cut(s) 703
PdmI GAANNNNTTC 1 cut(s) 101
PfeI GAWTC 5 cut(s) 389, 512, 776, 932, 1148
PflMI CCANNNNNTGG 1 cut(s) 543
PfoI TCCNGGA 1 cut(s) 54
PleI GAGTC 2 cut(s) 739, 1492
PmaCI CACGTG 1 cut(s) 1160
PmlI CACGTG 1 cut(s) 1160
PpsI GAGTC 2 cut(s) 739, 1492
Ppu21I YACGTR 1 cut(s) 1160
PscI ACATGT 2 cut(s) 617, 1187
PsiI TTATAA 1 cut(s) 626
Psp124BI GAGCTC 1 cut(s) 26
Psp6I CCWGG 5 cut(s) 54, 124, 507, 1717, 1788
PspCI CACGTG 1 cut(s) 1160
PspGI CCWGG 5 cut(s) 54, 124, 507, 1717, 1788
PspN4I GGNNCC 3 cut(s) 505, 1123, 1515
PspPI GGNCC 2 cut(s) 407, 468
PstI CTGCAG 2 cut(s) 382, 1683
PstNI CAGNNNCTG 2 cut(s) 305, 1145
PsuI RGATCY 3 cut(s) 667, 1294, 1513
PteI GCGCGC 2 cut(s) 1369, 1462
PvuII CAGCTG 1 cut(s) 1172
RsaI GTAC 9 cut(s) 548, 568, 820, 992, 1315, 1330, 1423, 1501, 1732
RsaNI GTAC 9 cut(s) 547, 567, 819, 991, 1314, 1329, 1422, 1500, 1731
RseI CAYNNNNRTG 2 cut(s) 294, 1585
SacI GAGCTC 1 cut(s) 26
SaqAI TTAA 7 cut(s) 461, 770, 882, 975, 1050, 1131, 1337
Sau3AI GATC 8 cut(s) 667, 681, 735, 985, 1105, 1246, 1294, 1513
Sau96I GGNCC 2 cut(s) 407, 468
ScaI AGTACT 3 cut(s) 992, 1315, 1330
SchI GAGTC 2 cut(s) 740, 1493
ScrFI CCNGG 5 cut(s) 56, 126, 509, 1719, 1790
SduI GDGCHC 2 cut(s) 26, 508
SfaNI GCATC 3 cut(s) 523, 709, 1132
SfcI CTRYAG 3 cut(s) 378, 1031, 1679
SfuI TTCGAA 2 cut(s) 76, 1536
SinI GGWCC 2 cut(s) 407, 468
SmiMI CAYNNNNRTG 2 cut(s) 294, 1585
SpeI ACTAGT 1 cut(s) 836
SsiI CCGC 5 cut(s) 39, 650, 966, 1459, 1472
SspI AATATT 1 cut(s) 1645
SspMI CTAG 6 cut(s) 827, 837, 1298, 1317, 1520, 1653
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 5 cut(s) 54, 124, 507, 1717, 1788
StyI CCWWGG 2 cut(s) 1479, 1701
TaaI ACNGT 4 cut(s) 584, 1212, 1546, 1587
TaiI ACGT 1 cut(s) 1162
TaqI TCGA 6 cut(s) 76, 499, 684, 734, 1536, 1592
TatI WGTACW 3 cut(s) 990, 1313, 1328
TauI GCSGC 2 cut(s) 1462, 1475
TfiI GAWTC 5 cut(s) 389, 512, 776, 932, 1148
Tru1I TTAA 7 cut(s) 461, 770, 882, 975, 1050, 1131, 1337
Tru9I TTAA 7 cut(s) 461, 770, 882, 975, 1050, 1131, 1337
TscAI CASTG 3 cut(s) 112, 331, 1590
TseFI GTSAC 3 cut(s) 14, 128, 584
Tsp45I GTSAC 3 cut(s) 14, 128, 584
TspDTI ATGAA 7 cut(s) 360, 378, 546, 593, 797, 1412, 1541
TspGWI ACGGA 2 cut(s) 960, 1293
TspRI CASTG 3 cut(s) 112, 331, 1590
Van91I CCANNNNNTGG 1 cut(s) 543
VpaK11BI GGWCC 2 cut(s) 407, 468
XagI CCTNNNNNAGG 1 cut(s) 463
XapI RAATTY 5 cut(s) 47, 146, 338, 368, 977
XceI RCATGY 2 cut(s) 621, 1191
XmiI GTMKAC 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 101
XspI CTAG 6 cut(s) 827, 837, 1298, 1317, 1520, 1653
ZrmI AGTACT 3 cut(s) 992, 1315, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.