Rh4DG405900

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0018032
Physical Location & Seq
Reverse (-)
61995878 .. 61998629
2752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG405900.1

Sequence Viewer

Length: 2067 bp
ATGGCAGCTGAAGGTGAGACGAGCTCATCCACACTGCGCGATAGAGAGTTTCTCCTGGAAATTGAGGCTAAAGTTCGAGAATGGTGGGAAAACAAGAAAGTTTTCAGTGCTGAATGTGAAAAGCCTGGTTACAAATTCTTTGGAAAATTCCCCTTTGATAGCTTTATGGGAAATCCATTCTCACTCTCCAAGCTAGACCAGTCTGCATATCTACCAGCTTTTCCTGTTGATGTCATGCGATTTGCTTTGGCGTATGTTTGTGATGGTACTGTTGATGAGGCGAAAATCAACTTTCTACATAGCATTGCAGATTCTGTATTTGATACTGCAACTACTGCCATCCTAAAATTCACTGAAGAGATATCATGGCATGAAGAATTTTTGGCTGCAGATTTTGATTCTTGTTTGAGATGTGGTCCCCCTTCTACTTTCGCAGACAAGGTGTTTGCCAATGAGATAAACATTGCTCTGAACAGGACCAAGCAGCAATACGAGGCTGACCAGTTTCGAGAATCCCTGGATTCTATTTTTGAGCTTCAATATGCCAGAAATTGGTACAAGAGTTCATGTGGTGGTACAAATGCAATGAACCGTGACTTAGTGTGGCGTTTTATGGATGTGCAGACATGTCTTATAACTCCAATCTGTCCACACTATGCGGAATATGTCTGGAGGGATCTTTTGTACAAGATAGACTTTGTGGTGAATGCAGACTGGCCTGTGGCTGATGCTCCAGATGATTCAACCCTCCAGAGTGCCAATAAGTATTTGAAAGACTTAATTGATTCAATGAAGAAGCAGACTGAAATGGAGAGAAATAAGAAGGGTACTGCTCCAGCTACATCTAGTTTGATAGAAGAAAGCCAAAACTTAGAAGCTCTTATATACGTTAATGAGGCATGTGATACTCTTACTTTTGCTACAGAGGGATTGCATACAGATTATGGACAAAACGAAGATGAGTTGAAGCAGAATGAGGGGGTTAAATTTGGGGCTCAAGCCTTAGACTTGAAGCTGCCATTTAGAGAGATTAGAGTACTTCTACAGAATTTGGACTTTATTAAGAGGGAAGTCGGTCTTGACAAGGTGCAAGTTTTATCAGCTACCAACCCTGAAGATCTTGCTAAAGCTGGTTCTCCGGTTAAAATAATAGAAGCTGAATCTTCCTCACCTGGAAATCCAGCTATTCTCTTCTTGACATGTCCAAAAAGTGATTGTGGATCTTTTGGTACTTCTAAGACTACACCAATAATGGCCGCTCTATCCTCATCTGGTTGTGCGCGAACGCACATCAACCCTTTAACCAAGTTCATGTCGAAGCCAAAGTGTAATGATGAGAAGAGTCGGGGTCCAGAATGGACTGATGAGCAAAGGGAAGAAAAACATCTTGCATGGGAAACATCTGACCACCATGATACATCCACTATAGATGAGAAGTCTCGAGATAATCCTGCTTCCTTGTATCTCCCTCCCTCTAAGCTACTCTTCCAGAGTACCTTTGATGAGGCATTGGATACTGCTTGTAATCAAGACAAATGGCTCCTGGTGAACATGCAATGTGTGGATAACTTCAGCTCACATATGCTTAATCAACACATCTGGGCTGAAGAAGCTGTCTCTGAGATCATTAGTACCAATTTTATCTTTTGGCATGAGTATTTTGATACACCTGAAGGTAAGATGGTTGCCAGAAAGTACTGGTTGGACTCAAGAACCATAGTACTCCTCATTGACCCCATGACTGGTGAAGCAATGTGTTCATGGAATGGAATGGTTCATCGGAATGATTTTGTGGAGGATCTAGTACCATACCTGGAGAGAAGCCCCAAGAATAATTTCGTAACTAAAGAAGAAGAGAAGGAACTACTCGCATTGGCAGCTTCCATGGAAAGCATGGAGTCGGAGACAGGTAGCGGAAGTACGTCCAAAGAGGATCTAGTACCATTCTGGAATAGTAGCCCGTGGAGTACTTCTCCAACTCAAGAAGACGAGGAATTGGCAGTTTTCACGGAAAGCATGAAGTGGGAAACAAGTAGTCCAACTAGTTCCAAAGCAAAAGTCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

688

Amino Acids

77.93

Weight (kDa)

4.69

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anticodon_1 PF08264 146 - 275 3.8e-12 Anticodon-binding domain of tRNA ligase
Thioredoxin_7 PF13899 497 - 561 1.3e-08 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018032)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33690 FvH4_4g33700
rosa_chinensis RchiOBHm_Chr4g0443171
rosa_laevigata RLG00000005950
rosa_multiflora Rmu_sc0007968.1_g000006
rosa_roxburghii Rroxscaffold_5G00383770
rosa_rugosa Rorug04G0344700
rosa_samantha Rh4BG410800 Rh4CG426300 Rh4DG405900
rosa_wichuraiana Rw4G034420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
AccB7I CCANNNNNTGG 1 cut(s) 552
AccBSI CCGCTC 1 cut(s) 1259
AccI GTMKAC 1 cut(s) 2061
AccII CGCG 2 cut(s) 39, 1282
AciI CCGC 3 cut(s) 659, 1257, 1914
AclWI GGATC 4 cut(s) 684, 1228, 1806, 1941
AcoI YGGCCR 1 cut(s) 1254
AcsI RAATTY 6 cut(s) 134, 146, 347, 377, 986, 1048
AcuI CTGAAG 6 cut(s) 30, 375, 1134, 1555, 1626, 1692
AfiI CCNNNNNNNGG 2 cut(s) 552, 1742
AflIII ACRYGT 2 cut(s) 626, 1199
AgsI TTSAA 6 cut(s) 539, 744, 772, 789, 967, 1012
AhlI ACTAGT 1 cut(s) 2042
AjnI CCWGG 6 cut(s) 54, 124, 516, 1171, 1542, 1812
AjuI GAANNNNNNNTTGG 2 cut(s) 1820, 1852
Alw21I GWGCWC 1 cut(s) 26
Alw26I GTCTC 4 cut(s) 11, 1443, 1621, 1898
AlwI GGATC 4 cut(s) 684, 1228, 1806, 1941
AlwNI CAGNNNCTG 1 cut(s) 314
Ama87I CYCGRG 1 cut(s) 1440
AoxI GGCC 2 cut(s) 716, 1254
ApeKI GCWGC 5 cut(s) 5, 386, 484, 1015, 1877
ApoI RAATTY 6 cut(s) 134, 146, 347, 377, 986, 1048
ArsI GACNNNNNNTTYG 3 cut(s) 428, 460, 2043
Asp700I GAANNNNTTC 2 cut(s) 101, 1835
AspLEI GCGC 2 cut(s) 39, 1282
AspS9I GGNCC 3 cut(s) 416, 477, 1349
AsuHPI GGTGA 5 cut(s) 26, 715, 1161, 1558, 1757
AvaI CYCGRG 1 cut(s) 1440
AvaII GGWCC 3 cut(s) 416, 477, 1349
BaeI ACNNNNGTAYC 2 cut(s) 547, 580
BanII GRGCYC 2 cut(s) 26, 997
BbsI GAAGAC 1 cut(s) 1992
Bbv12I GWGCWC 1 cut(s) 26
BbvI GCAGC 5 cut(s) 17, 373, 496, 1002, 1889
BccI CCATC 3 cut(s) 257, 347, 1675
BciT130I CCWGG 6 cut(s) 56, 126, 518, 1173, 1544, 1814
BciVI GTATCC 1 cut(s) 1507
BcoDI GTCTC 4 cut(s) 11, 1443, 1621, 1898
BcuI ACTAGT 1 cut(s) 2042
BfaI CTAG 6 cut(s) 194, 846, 1802, 1937, 2043, 2065
BfmI CTRYAG 4 cut(s) 387, 921, 1043, 1425
BfuI GTATCC 1 cut(s) 1507
BglII AGATCT 1 cut(s) 1117
BisI GCNGC 6 cut(s) 6, 387, 485, 1016, 1257, 1878
BlsI GCNGC 6 cut(s) 7, 388, 486, 1017, 1258, 1879
BmcAI AGTACT 4 cut(s) 1038, 1697, 1722, 1969
Bme1390I CCNGG 6 cut(s) 56, 126, 518, 1173, 1544, 1814
Bme18I GGWCC 3 cut(s) 416, 477, 1349
BmeT110I CYCGRG 1 cut(s) 1440
BmgT120I GGNCC 3 cut(s) 416, 477, 1349
BmiI GGNNCC 3 cut(s) 418, 1350, 1541
BmrFI CCNGG 6 cut(s) 56, 126, 518, 1173, 1544, 1814
BmsI GCATC 1 cut(s) 718
BpiI GAAGAC 1 cut(s) 1992
BplI GAGNNNNNCTC 6 cut(s) 8, 40, 36, 68, 1957, 1989
BpmI CTGGAG 5 cut(s) 691, 717, 734, 819, 1835
BpuEI CTTGAG 3 cut(s) 981, 1693, 1965
BsaJI CCNNGG 3 cut(s) 516, 1884, 1961
BsaWI WCCGGW 1 cut(s) 1138
Bsc4I CCNNNNNNNGG 2 cut(s) 552, 1742
Bse1I ACTGG 5 cut(s) 199, 502, 719, 1703, 1747
Bse3DI GCAATG 5 cut(s) 303, 462, 591, 1562, 1758
BseBI CCWGG 6 cut(s) 56, 126, 518, 1173, 1544, 1814
BseDI CCNNGG 3 cut(s) 516, 1884, 1961
BseGI GGATG 4 cut(s) 26, 339, 622, 1418
BseLI CCNNNNNNNGG 2 cut(s) 552, 1742
BseMI GCAATG 5 cut(s) 303, 462, 591, 1562, 1758
BseMII CTCAG 1 cut(s) 1611
BseNI ACTGG 5 cut(s) 199, 502, 719, 1703, 1747
BseRI GAGGAG 1 cut(s) 1715
BseXI GCAGC 5 cut(s) 17, 373, 496, 1002, 1889
BsgI GTGCAG 1 cut(s) 641
Bsh1236I CGCG 2 cut(s) 39, 1282
BshFI GGCC 2 cut(s) 718, 1256
BsiHKAI GWGCWC 1 cut(s) 26
BsiHKCI CYCGRG 1 cut(s) 1440
BsiSI CCGG 1 cut(s) 1139
BslFI GGGAC 1 cut(s) 402
BslI CCNNNNNNNGG 2 cut(s) 552, 1742
BsmAI GTCTC 4 cut(s) 11, 1443, 1621, 1898
BsmBI CGTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 402
BsmI GAATGC 1 cut(s) 712
BsnI GGCC 2 cut(s) 718, 1256
BsoBI CYCGRG 1 cut(s) 1440
Bsp1286I GDGCHC 2 cut(s) 26, 997
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 6 cut(s) 676, 1117, 1220, 1623, 1798, 1933
Bsp19I CCATGG 1 cut(s) 1884
BspACI CCGC 3 cut(s) 659, 1257, 1914
BspANI GGCC 2 cut(s) 718, 1256
BspCNI CTCAG 1 cut(s) 1612
BspFNI CGCG 2 cut(s) 39, 1282
BspLI GGNNCC 3 cut(s) 418, 1350, 1541
BspMAI CTGCAG 1 cut(s) 391
BspPI GGATC 4 cut(s) 684, 1228, 1806, 1941
BsrBI CCGCTC 1 cut(s) 1259
BsrDI GCAATG 5 cut(s) 303, 462, 591, 1562, 1758
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 5 cut(s) 199, 502, 719, 1703, 1747
BssECI CCNNGG 3 cut(s) 516, 1884, 1961
BssMI GATC 6 cut(s) 676, 1117, 1220, 1623, 1798, 1933
BssT1I CCWWGG 1 cut(s) 1884
Bst2UI CCWGG 6 cut(s) 56, 126, 518, 1173, 1544, 1814
Bst4CI ACNGT 2 cut(s) 271, 593
Bst6I CTCTTC 5 cut(s) 351, 1196, 1334, 1490, 1848
BstAPI GCANNNNNTGC 1 cut(s) 335
BstAUI TGTACA 1 cut(s) 684
BstDEI CTNAG 6 cut(s) 598, 871, 1003, 1236, 1476, 1620
BstDSI CCRYGG 2 cut(s) 1884, 1961
BstF5I GGATG 4 cut(s) 26, 339, 622, 1418
BstFNI CGCG 2 cut(s) 39, 1282
BstHHI GCGC 2 cut(s) 39, 1282
BstKTI GATC 6 cut(s) 679, 1120, 1223, 1626, 1801, 1936
BstMAI GTCTC 4 cut(s) 11, 1443, 1621, 1898
BstMBI GATC 6 cut(s) 676, 1117, 1220, 1623, 1798, 1933
BstMWI GCNNNNNNNGC 3 cut(s) 335, 1610, 1877
BstNI CCWGG 6 cut(s) 56, 126, 518, 1173, 1544, 1814
BstNSI RCATGY 4 cut(s) 630, 903, 1203, 1555
BstSCI CCNGG 6 cut(s) 54, 124, 516, 1171, 1542, 1812
BstSFI CTRYAG 4 cut(s) 387, 921, 1043, 1425
BstUI CGCG 2 cut(s) 39, 1282
BstV1I GCAGC 5 cut(s) 17, 373, 496, 1002, 1889
BstV2I GAAGAC 1 cut(s) 1992
BstX2I RGATCY 5 cut(s) 676, 1117, 1220, 1798, 1933
BstYI RGATCY 5 cut(s) 676, 1117, 1220, 1798, 1933
BsuI GTATCC 1 cut(s) 1507
BsuRI GGCC 2 cut(s) 718, 1256
BtgI CCRYGG 2 cut(s) 1884, 1961
BtsCI GGATG 4 cut(s) 26, 339, 622, 1418
BtsI GCAGTG 1 cut(s) 32
BtsIMutI CAGTG 3 cut(s) 32, 112, 351
CaiI CAGNNNCTG 1 cut(s) 314
CfoI GCGC 2 cut(s) 39, 1282
Cfr13I GGNCC 3 cut(s) 416, 477, 1349
DdeI CTNAG 6 cut(s) 598, 871, 1003, 1236, 1476, 1620
DpnI GATC 6 cut(s) 678, 1119, 1222, 1625, 1800, 1935
DpnII GATC 6 cut(s) 676, 1117, 1220, 1623, 1798, 1933
EaeI YGGCCR 1 cut(s) 1254
Eam1104I CTCTTC 5 cut(s) 351, 1196, 1334, 1490, 1848
EarI CTCTTC 5 cut(s) 351, 1196, 1334, 1490, 1848
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 1884
Eco24I GRGCYC 2 cut(s) 26, 997
Eco32I GATATC 1 cut(s) 363
Eco47I GGWCC 3 cut(s) 416, 477, 1349
Eco53kI GAGCTC 1 cut(s) 24
Eco57I CTGAAG 6 cut(s) 30, 375, 1134, 1555, 1626, 1692
Eco88I CYCGRG 1 cut(s) 1440
EcoICRI GAGCTC 1 cut(s) 24
EcoRII CCWGG 6 cut(s) 54, 124, 516, 1171, 1542, 1812
EcoRV GATATC 1 cut(s) 363
EcoT14I CCWWGG 1 cut(s) 1884
EcoT38I GRGCYC 2 cut(s) 26, 997
ErhI CCWWGG 1 cut(s) 1884
Esp3I CGTCTC 1 cut(s) 11
FalI AAGNNNNNCTT 6 cut(s) 680, 712, 1062, 1094, 1469, 1501
FaqI GGGAC 1 cut(s) 402
FauNDI CATATG 1 cut(s) 1581
FblI GTMKAC 1 cut(s) 2061
Fnu4HI GCNGC 6 cut(s) 6, 387, 485, 1016, 1257, 1878
FokI GGATG 4 cut(s) 13, 326, 629, 1405
FriOI GRGCYC 2 cut(s) 26, 997
Fsp4HI GCNGC 6 cut(s) 6, 387, 485, 1016, 1257, 1878
FspBI CTAG 6 cut(s) 194, 846, 1802, 1937, 2043, 2065
GlaI GCGC 2 cut(s) 38, 1281
GluI GCNGC 6 cut(s) 6, 387, 485, 1016, 1257, 1878
GsuI CTGGAG 5 cut(s) 691, 717, 734, 819, 1835
HaeIII GGCC 2 cut(s) 718, 1256
HapII CCGG 1 cut(s) 1139
HhaI GCGC 2 cut(s) 39, 1282
Hin6I GCGC 2 cut(s) 37, 1280
HinP1I GCGC 2 cut(s) 37, 1280
HincII GTYRAC 1 cut(s) 2062
HindII GTYRAC 1 cut(s) 2062
HpaII CCGG 1 cut(s) 1139
HphI GGTGA 5 cut(s) 26, 715, 1161, 1558, 1757
Hpy166II GTNNAC 3 cut(s) 650, 1549, 2062
Hpy188I TCNGA 5 cut(s) 471, 1405, 1621, 1782, 1903
Hpy8I GTNNAC 3 cut(s) 650, 1549, 2062
HpyAV CCTTC 5 cut(s) 5, 432, 817, 1667, 1852
HpyCH4III ACNGT 2 cut(s) 271, 593
HpyCH4IV ACGT 2 cut(s) 888, 1922
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 1610, 1877
HpyF3I CTNAG 6 cut(s) 598, 871, 1003, 1236, 1476, 1620
HpySE526I ACGT 2 cut(s) 888, 1922
HspAI GCGC 2 cut(s) 37, 1280
Kzo9I GATC 6 cut(s) 676, 1117, 1220, 1623, 1798, 1933
LmnI GCTCC 3 cut(s) 736, 838, 1545
Lsp1109I GCAGC 5 cut(s) 17, 373, 496, 1002, 1889
LweI GCATC 1 cut(s) 718
MaeI CTAG 6 cut(s) 194, 846, 1802, 1937, 2043, 2065
MaeII ACGT 2 cut(s) 888, 1922
MaeIII GTNAC 3 cut(s) 128, 593, 1840
MalI GATC 6 cut(s) 678, 1119, 1222, 1625, 1800, 1935
MbiI CCGCTC 1 cut(s) 1259
MboI GATC 6 cut(s) 676, 1117, 1220, 1623, 1798, 1933
MflI RGATCY 5 cut(s) 676, 1117, 1220, 1798, 1933
MhlI GDGCHC 2 cut(s) 26, 997
MlyI GAGTC 3 cut(s) 1351, 1700, 1907
MmeI TCCRAC 4 cut(s) 1683, 1881, 2000, 2063
MroXI GAANNNNTTC 2 cut(s) 101, 1835
MseI TTAA 7 cut(s) 779, 891, 984, 1062, 1143, 1301, 1587
MslI CAYNNNNRTG 1 cut(s) 1782
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 1139
MspR9I CCNGG 6 cut(s) 56, 126, 518, 1173, 1544, 1814
Mva1269I GAATGC 1 cut(s) 712
MvaI CCWGG 6 cut(s) 56, 126, 518, 1173, 1544, 1814
MvnI CGCG 2 cut(s) 39, 1282
MwoI GCNNNNNNNGC 3 cut(s) 335, 1610, 1877
NcoI CCATGG 1 cut(s) 1884
NdeI CATATG 1 cut(s) 1581
NdeII GATC 6 cut(s) 676, 1117, 1220, 1623, 1798, 1933
NlaIV GGNNCC 3 cut(s) 418, 1350, 1541
NmuCI GTSAC 1 cut(s) 593
NspI RCATGY 4 cut(s) 630, 903, 1203, 1555
PaeR7I CTCGAG 1 cut(s) 1440
PciI ACATGT 2 cut(s) 626, 1199
PctI GAATGC 1 cut(s) 712
PdmI GAANNNNTTC 2 cut(s) 101, 1835
PfeI GAWTC 7 cut(s) 311, 398, 512, 521, 740, 785, 1160
PflMI CCANNNNNTGG 1 cut(s) 552
PfoI TCCNGGA 1 cut(s) 54
PkrI GCNGC 6 cut(s) 7, 388, 486, 1017, 1258, 1879
PleI GAGTC 3 cut(s) 1350, 1700, 1906
PpsI GAGTC 3 cut(s) 1350, 1700, 1906
PscI ACATGT 2 cut(s) 626, 1199
PsiI TTATAA 1 cut(s) 635
Psp124BI GAGCTC 1 cut(s) 26
Psp6I CCWGG 6 cut(s) 54, 124, 516, 1171, 1542, 1812
PspGI CCWGG 6 cut(s) 54, 124, 516, 1171, 1542, 1812
PspN4I GGNNCC 3 cut(s) 418, 1350, 1541
PspPI GGNCC 3 cut(s) 416, 477, 1349
PstI CTGCAG 1 cut(s) 391
PstNI CAGNNNCTG 1 cut(s) 314
PsuI RGATCY 5 cut(s) 676, 1117, 1220, 1798, 1933
PvuII CAGCTG 1 cut(s) 8
RseI CAYNNNNRTG 1 cut(s) 1782
SacI GAGCTC 1 cut(s) 26
SalI GTCGAC 1 cut(s) 2060
SaqAI TTAA 7 cut(s) 779, 891, 984, 1062, 1143, 1301, 1587
SatI GCNGC 6 cut(s) 6, 387, 485, 1016, 1257, 1878
Sau3AI GATC 6 cut(s) 676, 1117, 1220, 1623, 1798, 1933
Sau96I GGNCC 3 cut(s) 416, 477, 1349
ScaI AGTACT 4 cut(s) 1038, 1697, 1722, 1969
SchI GAGTC 3 cut(s) 1351, 1700, 1907
ScrFI CCNGG 6 cut(s) 56, 126, 518, 1173, 1544, 1814
SduI GDGCHC 2 cut(s) 26, 997
SfaNI GCATC 1 cut(s) 718
SfcI CTRYAG 4 cut(s) 387, 921, 1043, 1425
Sfr274I CTCGAG 1 cut(s) 1440
SinI GGWCC 3 cut(s) 416, 477, 1349
SlaI CTCGAG 1 cut(s) 1440
SmiMI CAYNNNNRTG 1 cut(s) 1782
SmlI CTYRAG 4 cut(s) 996, 1440, 1708, 1980
SmoI CTYRAG 4 cut(s) 996, 1440, 1708, 1980
SpeI ACTAGT 1 cut(s) 2042
SsiI CCGC 3 cut(s) 659, 1257, 1914
SspMI CTAG 6 cut(s) 194, 846, 1802, 1937, 2043, 2065
SstI GAGCTC 1 cut(s) 26
StyD4I CCNGG 6 cut(s) 54, 124, 516, 1171, 1542, 1812
StyI CCWWGG 1 cut(s) 1884
TaaI ACNGT 2 cut(s) 271, 593
TaiI ACGT 2 cut(s) 891, 1925
TaqI TCGA 5 cut(s) 76, 508, 1316, 1441, 2061
TaqII GACCGA 1 cut(s) 1064
TatI WGTACW 5 cut(s) 684, 1036, 1695, 1720, 1967
TauI GCSGC 1 cut(s) 1259
TfiI GAWTC 7 cut(s) 311, 398, 512, 521, 740, 785, 1160
Tru1I TTAA 7 cut(s) 779, 891, 984, 1062, 1143, 1301, 1587
Tru9I TTAA 7 cut(s) 779, 891, 984, 1062, 1143, 1301, 1587
TscAI CASTG 3 cut(s) 39, 112, 358
TseFI GTSAC 1 cut(s) 593
TseI GCWGC 5 cut(s) 5, 386, 484, 1015, 1877
Tsp45I GTSAC 1 cut(s) 593
TspDTI ATGAA 8 cut(s) 387, 555, 602, 806, 1300, 1749, 1766, 2033
TspGWI ACGGA 1 cut(s) 2024
TspRI CASTG 3 cut(s) 39, 112, 358
Van91I CCANNNNNTGG 1 cut(s) 552
VpaK11BI GGWCC 3 cut(s) 416, 477, 1349
XapI RAATTY 6 cut(s) 134, 146, 347, 377, 986, 1048
XceI RCATGY 4 cut(s) 630, 903, 1203, 1555
XcmI CCANNNNNNNNNTGG 1 cut(s) 1891
XhoI CTCGAG 1 cut(s) 1440
XmiI GTMKAC 1 cut(s) 2061
XmnI GAANNNNTTC 2 cut(s) 101, 1835
XspI CTAG 6 cut(s) 194, 846, 1802, 1937, 2043, 2065
ZrmI AGTACT 4 cut(s) 1038, 1697, 1722, 1969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.