FvH4_4g34140

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010181
Physical Location & Seq
Forward (+)
32089631 .. 32096973
7343 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34140.t3

Sequence Viewer

Length: 2451 bp
ATGAGCGGGAACGAGTTCCGGTTCTTCTTGTCCTGCGACATCAATCTTCCACTGACCTTTCGGATCGAGCGGCTCGAAGGAACTCTGACCCGGCCCAAATCCGCAACCGCCGATGTTGATCCGACTCCGGCGACGGAGGAGAAAAGATCGGAGCTCTATGTGGAGTGCGCACTGTACATTGATGGTGCTCCGTTTGGCTTGCCCACTCGGACCAGGCTGGAATCTTCTGGACCTTCTTATTGTTGGAACGAGCTCATTACTCTGAGTACTAAGTATAGGGACTTGACTGGTCACTCACAGCTTGCTTTAACTGTTTGGGATGTTTCGTGTGGGAAAGATGAAGGGTTGATTGGTGGCGCTACGATTCTTCTGTTTAATAACAAAAAGCAACTCAAAACAGGGAAGCAGAAGCTGAGGCTTTGGCAAGGAAAATTAGCTGATGGATCATTTCCTACTTCGACTCCTGGGAAGGTCCCAAGGAATGAACGTGGGGAGTTGGAACGATTGGAGAAGCTAATGAATAAGTATGAGAGGGGACAGATCCAATGTGTTGATTGGCTGGACCGCCTTGCCTTTAAAGCTATGGAGAGAATAAAGGAACGTGAAAGCTCTAAAAATGGAAGTTTGCATTTGTACCTGGTTGTCGATTTCTGTAGTTTTGAGCATCGTGTTGTTTTCCAGGAATCAGGAGCAAATTTCTTACTACCATCCCCTATAGCTTCCACAAATGAAATTGTCACTGTATGGGACCCGGAGCTGGGAAGAATAAACCCATCAGAGCACAAGCAACTAAAACTTGCAAGGAGCTTAACCCGTGGTATCATTGATAGGGATTTGAAGCCTAGCTCCAATGAGAGAAAGTCGATACAGAGGATTCTAAAGTACCCTCCAACAAGGACACTGAGTGGTGACGAAAGACAGCTGCTGTGGAAATTTCGTTTCTCCTTGATGTCTGAGAAGAGGGCCCTCACAAAGTTCCTCAAATGTGTTGAATGGAGCGATGTTCAGGAAGCAAAGCAAGCATTAGAATTGATGGGCAGATGGGAAATGATTGATGTTTGCGATGCGTTGGAGCTCTTATCTCCTGTTTTCGAAAGTGAAGAGGTTCGTGCTTATGCTGTCAGTGTGCTTGAAAGAGCTGATGACGAAGAACTCCAGTGCTACTTACTTCAACTGGTTCAAGCACTTCGATTTGAGCGCTCTGATAAATCCTGCCTGTCTCATTTCCTTGTTCAGCGTGCATTACGAAACATTGAGTTGGCTAGCTTTCTTCGCTGGTATGTTGCAGTTGAACTTCATGATCCAGCATATGCGAAGCGTTTTTACTGTACCTATGAAATTCTGGAAGAGAATATGATGAAGTTGTCAGCTGGTATGCATGGAGATGAGGATGGTTTTAAATTGTGGCAAAGTTTGGTGCGTCAGACTGAATTGACTGCACAACTGTGTTCAATTACGAGAGATGTAAGAAACGTACGTGGCAATACTCAGAAGAAAATTGAAAAGCTTAGACAGCTGCTTTCTGGTCTTCTTAGTGAACTCACATATTTTGAAGAGCCGATTAGGTCACCACTGGCCCCAGGAGTCCTCATCACTGGAATCATACCATCCGAGTCATCAATATTCAAAAGTGCACTGCATCCTCTTCGCTTGACTTTCCGAGCAGCCAATGGGGGCAGTTGCAAAGTTATCTTCAAGAAGGGGGATGACATGCGCCAGGACCAATTGGTTGTTCAAATGGTATCTCTCATGGATCGATTGCTCAAGAATGAAAATCTGGATCTTCACTTAACTCCTTATAAGGTTCTTGCAACTGGGCAAGACGAAGGAATGATGGAGTTCATTCCATCCCGCACGCTAGCGCTGATTCTCTCAGAGCATAGAAGTATTACGAGCTACCTGCAGAAGTTTCATCCAGATGAGCATGGACCATTTGGTATTACTGCCACCTGTCTGGAAACATTCATAAAAAGCTGTGCTGGCTACTCTGTTATCACATATGTATTGGGCATTGGAGACAGGCATCTTGACAACCTTCTCCTTAGGGATGATGGAAGCCTTTTCCATGTTGATTTTGGCTTCATTCTTGGTCGTGATCCAAAGCCATTTCCACCACCAATGAAGCTTTGCAAAGAAATGGTTGAAGCTATGGGTGGAGCTGAAAGCCAGTACTATACTAGGTTCAAGTCCTATTGTTGTGAAGCATACAACATAATTCGGAAATCTAGTAACCTGATCTTGAATCTTTTTCATCTAATGGCGGGTTCCAACATTCCTGACATAGCTTCTGATCCTGAAAAAGGCATCCTCAAGCTCCAGGAGAAATTCCGGTTGGACTTGGATGATGAAGCCTGCATACATTTTTTCCAAGACTTGATCAATGAGAGTGTTAGTGCACTGTTTCCTCAAATGGTTGAGACCATTCATCGGTGGGCTCAATATTGGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000407 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005777 GO:0005942 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006914 GO:0006950 GO:0006970 GO:0006996 GO:0007033 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009056 GO:0009058 GO:0009555 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016236 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019637 GO:0019898 GO:0022607 GO:0023052 GO:0030242 GO:0030258 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0034271 GO:0034272 GO:0035004 GO:0035032 GO:0035556 GO:0036092 GO:0036211 GO:0042579 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045017 GO:0046474 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0052742 GO:0055046 GO:0061695 GO:0061919 GO:0065007 GO:0070925 GO:0071704 GO:0071840 GO:0072593 GO:0090407 GO:0097708 GO:0098657 GO:0098796 GO:1901564 GO:1901576 GO:1902494 GO:1905037 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 ko04136 ko04138 ko04140 ko04145 ko04371 ko05152 ko05167 map00562 map01100 map04070 map04136 map04138 map04140 map04145 map04371 map05152 map05167
Pfam Domains
Protein Families
PI3K_C2 PI3Ka PI3_PI4_kinase

Protein Analysis

817

Amino Acids

93.38

Weight (kDa)

6.54

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3K_C2 PF00792 50 - 192 5.2e-43 Phosphoinositide 3-kinase C2
PI3Ka PF00613 278 - 454 1.6e-66 Phosphoinositide 3-kinase family, accessory domain (PIK domain)
PI3_PI4_kinase PF00454 561 - 762 1.7e-58 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1800
Acc16I TGCGCA 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 1908
AccBSI CCGCTC 2 cut(s) 6, 70
AciI CCGC 7 cut(s) 6, 70, 102, 108, 565, 1852, 2261
AclWI GGATC 9 cut(s) 71, 113, 451, 535, 1295, 1761, 1788, 2090, 2285
AcsI RAATTY 4 cut(s) 694, 932, 1338, 2324
AdeI CACNNNGTG 1 cut(s) 905
AfaI GTAC 7 cut(s) 176, 268, 635, 884, 1330, 1476, 2171
AfeI AGCGCT 2 cut(s) 1199, 1863
AfiI CCNNNNNNNGG 4 cut(s) 757, 758, 2300, 2427
AjnI CCWGG 7 cut(s) 212, 463, 636, 678, 1579, 1716, 2316
AjuI GAANNNNNNNTTGG 6 cut(s) 333, 365, 1716, 1748, 2315, 2347
AleI CACNNNNGTG 1 cut(s) 1444
Alw21I GWGCWC 7 cut(s) 156, 190, 255, 783, 1077, 1636, 2398
Alw26I GTCTC 3 cut(s) 1224, 2010, 2411
Alw44I GTGCAC 2 cut(s) 1632, 2394
AlwI GGATC 9 cut(s) 71, 113, 451, 535, 1295, 1761, 1788, 2090, 2285
AlwNI CAGNNNCTG 2 cut(s) 412, 925
Aor51HI AGCGCT 2 cut(s) 1199, 1863
AoxI GGCC 3 cut(s) 92, 963, 1575
ApaI GGGCCC 1 cut(s) 967
ApaLI GTGCAC 2 cut(s) 1632, 2394
ApeKI GCWGC 3 cut(s) 922, 1516, 1664
ApoI RAATTY 4 cut(s) 694, 932, 1338, 2324
Asp700I GAANNNNTTC 2 cut(s) 14, 1104
AspLEI GCGC 6 cut(s) 170, 359, 1200, 1716, 1864, 2448
AsuC2I CCSGG 2 cut(s) 91, 752
AsuHPI GGTGA 2 cut(s) 920, 1560
AsuII TTCGAA 1 cut(s) 1092
AsuNHI GCTAGC 2 cut(s) 1262, 1858
AvaII GGWCC 7 cut(s) 210, 230, 472, 562, 748, 1720, 1928
AxyI CCTNAGG 1 cut(s) 2042
BaeGI GKGCMC 3 cut(s) 967, 1636, 2398
BanII GRGCYC 5 cut(s) 156, 255, 967, 1077, 2437
BarI GAAGNNNNNNTAC 4 cut(s) 1307, 1339, 2340, 2372
BbsI GAAGAC 1 cut(s) 1520
Bbv12I GWGCWC 7 cut(s) 156, 190, 255, 783, 1077, 1636, 2398
BbvCI CCTCAGC 1 cut(s) 413
BbvI GCAGC 3 cut(s) 909, 1503, 1676
BcgI CGANNNNNNTGC 4 cut(s) 1628, 1662, 1882, 1916
BciT130I CCWGG 7 cut(s) 214, 465, 638, 680, 1581, 1718, 2318
BclI TGATCA 1 cut(s) 2376
BcnI CCSGG 2 cut(s) 91, 752
BcoDI GTCTC 3 cut(s) 1224, 2010, 2411
BfaI CTAG 5 cut(s) 843, 1263, 1859, 2178, 2226
BfmI CTRYAG 3 cut(s) 652, 714, 1901
BfoI RGCGCY 4 cut(s) 360, 1201, 1865, 2449
BfuAI ACCTGC 1 cut(s) 1908
BisI GCNGC 4 cut(s) 71, 923, 1517, 1665
BlsI GCNGC 4 cut(s) 72, 924, 1518, 1666
BmcAI AGTACT 2 cut(s) 268, 2171
Bme1390I CCNGG 9 cut(s) 91, 214, 465, 638, 680, 752, 1581, 1718, 2318
Bme18I GGWCC 7 cut(s) 210, 230, 472, 562, 748, 1720, 1928
BmiI GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2266
BmrFI CCNGG 9 cut(s) 91, 214, 465, 638, 680, 752, 1581, 1718, 2318
BmrI ACTGGG 1 cut(s) 1824
BmsI GCATC 5 cut(s) 673, 1054, 1648, 2032, 2313
BmtI GCTAGC 2 cut(s) 1266, 1862
BmuI ACTGGG 1 cut(s) 1824
BpiI GAAGAC 1 cut(s) 1520
BpmI CTGGAG 2 cut(s) 1139, 2300
Bpu10I CCTNAGC 1 cut(s) 413
Bpu14I TTCGAA 1 cut(s) 1092
BpuEI CTTGAG 2 cut(s) 1748, 2294
BpuMI CCSGG 2 cut(s) 91, 752
Bsa29I ATCGAT 1 cut(s) 1756
BsaAI YACGTR 1 cut(s) 1478
BsaBI GATNNNNATC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 2411
BsaJI CCNNGG 4 cut(s) 464, 476, 814, 1579
BsaWI WCCGGW 2 cut(s) 18, 2328
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 4 cut(s) 757, 758, 2300, 2427
Bse1I ACTGG 7 cut(s) 292, 1156, 1179, 1578, 1600, 1819, 2167
Bse21I CCTNAGG 1 cut(s) 2042
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 7 cut(s) 214, 465, 638, 680, 1581, 1718, 2318
BseCI ATCGAT 1 cut(s) 1756
BseDI CCNNGG 4 cut(s) 464, 476, 814, 1579
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 4 cut(s) 757, 758, 2300, 2427
BseMII CTCAG 6 cut(s) 254, 404, 893, 945, 1502, 1887
BseNI ACTGG 7 cut(s) 292, 1156, 1179, 1578, 1600, 1819, 2167
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 3 cut(s) 967, 1636, 2398
BseXI GCAGC 3 cut(s) 909, 1503, 1676
BseYI CCCAGC 1 cut(s) 757
BsgI GTGCAG 1 cut(s) 1422
BshFI GGCC 3 cut(s) 94, 965, 1577
BshVI ATCGAT 1 cut(s) 1756
BsiHKAI GWGCWC 7 cut(s) 156, 190, 255, 783, 1077, 1636, 2398
BsiSI CCGG 5 cut(s) 19, 91, 128, 752, 2329
BsiWI CGTACG 1 cut(s) 1474
BslFI GGGAC 4 cut(s) 293, 458, 549, 761
BslI CCNNNNNNNGG 4 cut(s) 757, 758, 2300, 2427
BsmAI GTCTC 3 cut(s) 1224, 2010, 2411
BsmFI GGGAC 4 cut(s) 293, 458, 549, 761
BsnI GGCC 3 cut(s) 94, 965, 1577
Bso31I GGTCTC 1 cut(s) 2411
Bsp119I TTCGAA 1 cut(s) 1092
Bsp120I GGGCCC 1 cut(s) 963
Bsp1286I GDGCHC 9 cut(s) 156, 190, 255, 783, 967, 1077, 1636, 2398, 2437
Bsp1407I TGTACA 1 cut(s) 174
BspACI CCGC 7 cut(s) 6, 70, 102, 108, 565, 1852, 2261
BspANI GGCC 3 cut(s) 94, 965, 1577
BspCNI CTCAG 6 cut(s) 255, 405, 894, 946, 1501, 1886
BspDI ATCGAT 1 cut(s) 1756
BspHI TCATGA 1 cut(s) 1297
BspLI GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2266
BspMAI CTGCAG 1 cut(s) 1905
BspMI ACCTGC 1 cut(s) 1908
BspOI GCTAGC 2 cut(s) 1266, 1862
BspPI GGATC 9 cut(s) 71, 113, 451, 535, 1295, 1761, 1788, 2090, 2285
BspQI GCTCTTC 1 cut(s) 1548
BspT104I TTCGAA 1 cut(s) 1092
BspTNI GGTCTC 1 cut(s) 2411
BsrBI CCGCTC 2 cut(s) 6, 70
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 7 cut(s) 292, 1156, 1179, 1578, 1600, 1819, 2167
BssECI CCNNGG 4 cut(s) 464, 476, 814, 1579
BssT1I CCWWGG 1 cut(s) 476
Bst2UI CCWGG 7 cut(s) 214, 465, 638, 680, 1581, 1718, 2318
Bst4CI ACNGT 6 cut(s) 174, 313, 742, 1328, 1446, 2400
Bst6I CTCTTC 5 cut(s) 953, 1095, 1341, 1548, 1650
BstAUI TGTACA 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 1478
BstBI TTCGAA 1 cut(s) 1092
BstC8I GCNNGC 9 cut(s) 200, 303, 1020, 1239, 1264, 1856, 1860, 1981, 2353
BstDSI CCRYGG 1 cut(s) 814
BstEII GGTNACC 1 cut(s) 1566
BstENI CCTNNNNNAGG 1 cut(s) 2298
BstH2I RGCGCY 4 cut(s) 360, 1201, 1865, 2449
BstHHI GCGC 6 cut(s) 170, 359, 1200, 1716, 1864, 2448
BstMAI GTCTC 3 cut(s) 1224, 2010, 2411
BstMWI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
BstNI CCWGG 7 cut(s) 214, 465, 638, 680, 1581, 1718, 2318
BstNSI RCATGY 1 cut(s) 1714
BstPI GGTNACC 1 cut(s) 1566
BstSCI CCNGG 9 cut(s) 89, 212, 463, 636, 678, 750, 1579, 1716, 2316
BstSFI CTRYAG 3 cut(s) 652, 714, 1901
BstSLI GKGCMC 3 cut(s) 967, 1636, 2398
BstV1I GCAGC 3 cut(s) 909, 1503, 1676
BstV2I GAAGAC 1 cut(s) 1520
BstX2I RGATCY 2 cut(s) 540, 1780
BstXI CCANNNNNNTGG 1 cut(s) 1954
BstYI RGATCY 2 cut(s) 540, 1780
Bsu15I ATCGAT 1 cut(s) 1756
Bsu36I CCTNAGG 1 cut(s) 2042
BsuRI GGCC 3 cut(s) 94, 965, 1577
BsuTUI ATCGAT 1 cut(s) 1756
BtgI CCRYGG 1 cut(s) 814
BtgZI GCGATG 2 cut(s) 1014, 1077
BtsI GCAGTG 1 cut(s) 1634
BveI ACCTGC 1 cut(s) 1908
Cac8I GCNNGC 9 cut(s) 200, 303, 1020, 1239, 1264, 1856, 1860, 1981, 2353
CaiI CAGNNNCTG 2 cut(s) 412, 925
CciI TCATGA 1 cut(s) 1297
CfoI GCGC 6 cut(s) 170, 359, 1200, 1716, 1864, 2448
ClaI ATCGAT 1 cut(s) 1756
CseI GACGC 1 cut(s) 1409
CsiI ACCWGGT 1 cut(s) 636
Csp6I GTAC 7 cut(s) 175, 267, 634, 883, 1329, 1475, 2170
CviAII CATG 6 cut(s) 1298, 1379, 1711, 1750, 1925, 2066
CviQI GTAC 7 cut(s) 175, 267, 634, 883, 1329, 1475, 2170
DraI TTTAAA 2 cut(s) 577, 1399
DraIII CACNNNGTG 1 cut(s) 905
Eam1104I CTCTTC 5 cut(s) 953, 1095, 1341, 1548, 1650
EarI CTCTTC 5 cut(s) 953, 1095, 1341, 1548, 1650
Ecl136II GAGCTC 3 cut(s) 154, 253, 1075
Eco130I CCWWGG 1 cut(s) 476
Eco24I GRGCYC 5 cut(s) 156, 255, 967, 1077, 2437
Eco31I GGTCTC 1 cut(s) 2411
Eco47I GGWCC 7 cut(s) 210, 230, 472, 562, 748, 1720, 1928
Eco47III AGCGCT 2 cut(s) 1199, 1863
Eco53kI GAGCTC 3 cut(s) 154, 253, 1075
Eco81I CCTNAGG 1 cut(s) 2042
Eco91I GGTNACC 1 cut(s) 1566
EcoICRI GAGCTC 3 cut(s) 154, 253, 1075
EcoNI CCTNNNNNAGG 1 cut(s) 2298
EcoO109I RGGNCCY 4 cut(s) 472, 748, 963, 964
EcoO65I GGTNACC 1 cut(s) 1566
EcoRII CCWGG 7 cut(s) 212, 463, 636, 678, 1579, 1716, 2316
EcoT14I CCWWGG 1 cut(s) 476
EcoT22I ATGCAT 1 cut(s) 1380
EcoT38I GRGCYC 5 cut(s) 156, 255, 967, 1077, 2437
ErhI CCWWGG 1 cut(s) 476
FaeI CATG 6 cut(s) 1301, 1382, 1714, 1753, 1928, 2069
FaqI GGGAC 4 cut(s) 293, 458, 549, 761
FatI CATG 6 cut(s) 1297, 1378, 1710, 1749, 1924, 2065
FauI CCCGC 2 cut(s) 1859, 2254
FauNDI CATATG 2 cut(s) 1309, 1999
FbaI TGATCA 1 cut(s) 2376
Fnu4HI GCNGC 4 cut(s) 71, 923, 1517, 1665
FriOI GRGCYC 5 cut(s) 156, 255, 967, 1077, 2437
Fsp4HI GCNGC 4 cut(s) 71, 923, 1517, 1665
FspAI RTGCGCAY 1 cut(s) 169
FspBI CTAG 5 cut(s) 843, 1263, 1859, 2178, 2226
FspI TGCGCA 1 cut(s) 169
GlaI GCGC 6 cut(s) 169, 358, 1199, 1715, 1863, 2447
GluI GCNGC 4 cut(s) 71, 923, 1517, 1665
GsaI CCCAGC 1 cut(s) 761
GsuI CTGGAG 2 cut(s) 1139, 2300
HaeII RGCGCY 4 cut(s) 360, 1201, 1865, 2449
HaeIII GGCC 3 cut(s) 94, 965, 1577
HapII CCGG 5 cut(s) 19, 91, 128, 752, 2329
HgaI GACGC 1 cut(s) 1409
HhaI GCGC 6 cut(s) 170, 359, 1200, 1716, 1864, 2448
Hin1II CATG 6 cut(s) 1301, 1382, 1714, 1753, 1928, 2069
Hin6I GCGC 6 cut(s) 168, 357, 1198, 1714, 1862, 2446
HinP1I GCGC 6 cut(s) 168, 357, 1198, 1714, 1862, 2446
HindIII AAGCTT 2 cut(s) 1505, 2123
HpaII CCGG 5 cut(s) 19, 91, 128, 752, 2329
HphI GGTGA 2 cut(s) 920, 1560
Hpy166II GTNNAC 3 cut(s) 1538, 1634, 2396
Hpy8I GTNNAC 3 cut(s) 1538, 1634, 2396
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 7 cut(s) 71, 243, 335, 463, 1693, 1820, 2045
HpyCH4III ACNGT 6 cut(s) 174, 313, 742, 1328, 1446, 2400
HpyCH4IV ACGT 4 cut(s) 487, 601, 1473, 1477
HpyF10VI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
HpySE526I ACGT 4 cut(s) 487, 601, 1473, 1477
Hsp92II CATG 6 cut(s) 1301, 1382, 1714, 1753, 1928, 2069
HspAI GCGC 6 cut(s) 168, 357, 1198, 1714, 1862, 2446
KflI GGGWCCC 1 cut(s) 748
Ksp22I TGATCA 1 cut(s) 2376
LguI GCTCTTC 1 cut(s) 1548
Lsp1109I GCAGC 3 cut(s) 909, 1503, 1676
LweI GCATC 5 cut(s) 673, 1054, 1648, 2032, 2313
MabI ACCWGGT 1 cut(s) 636
MaeI CTAG 5 cut(s) 843, 1263, 1859, 2178, 2226
MaeII ACGT 4 cut(s) 487, 601, 1473, 1477
MaeIII GTNAC 5 cut(s) 290, 736, 908, 1566, 2228
MbiI CCGCTC 2 cut(s) 6, 70
MfeI CAATTG 1 cut(s) 1724
MflI RGATCY 2 cut(s) 540, 1780
MhlI GDGCHC 9 cut(s) 156, 190, 255, 783, 967, 1077, 1636, 2398, 2437
MlyI GAGTC 4 cut(s) 118, 454, 1593, 1622
MmeI TCCRAC 7 cut(s) 146, 224, 477, 914, 1050, 2292, 2313
Mph1103I ATGCAT 1 cut(s) 1380
MroXI GAANNNNTTC 2 cut(s) 14, 1104
MseI TTAA 6 cut(s) 308, 375, 576, 809, 1398, 1790
MslI CAYNNNNRTG 3 cut(s) 1383, 1444, 1917
MspA1I CMGCKG 3 cut(s) 922, 1370, 1516
MspI CCGG 5 cut(s) 19, 91, 128, 752, 2329
MspR9I CCNGG 9 cut(s) 91, 214, 465, 638, 680, 752, 1581, 1718, 2318
MunI CAATTG 1 cut(s) 1724
MvaI CCWGG 7 cut(s) 214, 465, 638, 680, 1581, 1718, 2318
MwoI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
NciI CCSGG 2 cut(s) 91, 752
NdeI CATATG 2 cut(s) 1309, 1999
NheI GCTAGC 2 cut(s) 1262, 1858
NlaIII CATG 6 cut(s) 1301, 1382, 1714, 1753, 1928, 2069
NlaIV GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2266
NmuCI GTSAC 4 cut(s) 290, 736, 908, 1566
NsbI TGCGCA 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 1380
NspI RCATGY 1 cut(s) 1714
NspV TTCGAA 1 cut(s) 1092
OliI CACNNNNGTG 1 cut(s) 1444
PagI TCATGA 1 cut(s) 1297
PciSI GCTCTTC 1 cut(s) 1548
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdmI GAANNNNTTC 2 cut(s) 14, 1104
PfeI GAWTC 7 cut(s) 221, 364, 683, 874, 1599, 1867, 2242
Pfl23II CGTACG 1 cut(s) 1474
PfoI TCCNGGA 2 cut(s) 678, 2316
PkrI GCNGC 4 cut(s) 72, 924, 1518, 1666
PleI GAGTC 4 cut(s) 118, 454, 1592, 1621
PpsI GAGTC 4 cut(s) 118, 454, 1592, 1621
Ppu21I YACGTR 1 cut(s) 1478
PpuMI RGGWCCY 2 cut(s) 472, 748
PsiI TTATAA 1 cut(s) 1800
Psp124BI GAGCTC 3 cut(s) 156, 255, 1077
Psp5II RGGWCCY 2 cut(s) 472, 748
Psp6I CCWGG 7 cut(s) 212, 463, 636, 678, 1579, 1716, 2316
PspEI GGTNACC 1 cut(s) 1566
PspFI CCCAGC 1 cut(s) 757
PspGI CCWGG 7 cut(s) 212, 463, 636, 678, 1579, 1716, 2316
PspLI CGTACG 1 cut(s) 1474
PspN4I GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2266
PspOMI GGGCCC 1 cut(s) 963
PspPPI RGGWCCY 2 cut(s) 472, 748
PstI CTGCAG 1 cut(s) 1905
PstNI CAGNNNCTG 2 cut(s) 412, 925
PsuI RGATCY 2 cut(s) 540, 1780
PvuII CAGCTG 3 cut(s) 922, 1370, 1516
RsaI GTAC 7 cut(s) 176, 268, 635, 884, 1330, 1476, 2171
RsaNI GTAC 7 cut(s) 175, 267, 634, 883, 1329, 1475, 2170
RseI CAYNNNNRTG 3 cut(s) 1383, 1444, 1917
SacI GAGCTC 3 cut(s) 156, 255, 1077
SapI GCTCTTC 1 cut(s) 1548
SaqAI TTAA 6 cut(s) 308, 375, 576, 809, 1398, 1790
SatI GCNGC 4 cut(s) 71, 923, 1517, 1665
ScaI AGTACT 2 cut(s) 268, 2171
SchI GAGTC 4 cut(s) 118, 454, 1593, 1622
ScrFI CCNGG 9 cut(s) 91, 214, 465, 638, 680, 752, 1581, 1718, 2318
SduI GDGCHC 9 cut(s) 156, 190, 255, 783, 967, 1077, 1636, 2398, 2437
SexAI ACCWGGT 1 cut(s) 636
SfaNI GCATC 5 cut(s) 673, 1054, 1648, 2032, 2313
SfcI CTRYAG 3 cut(s) 652, 714, 1901
SfuI TTCGAA 1 cut(s) 1092
SinI GGWCC 7 cut(s) 210, 230, 472, 562, 748, 1720, 1928
SmiMI CAYNNNNRTG 3 cut(s) 1383, 1444, 1917
SmlI CTYRAG 2 cut(s) 1763, 2309
SmoI CTYRAG 2 cut(s) 1763, 2309
SsiI CCGC 7 cut(s) 6, 70, 102, 108, 565, 1852, 2261
SspI AATATT 2 cut(s) 1623, 2441
SspMI CTAG 5 cut(s) 843, 1263, 1859, 2178, 2226
SstI GAGCTC 3 cut(s) 156, 255, 1077
StyD4I CCNGG 9 cut(s) 89, 212, 463, 636, 678, 750, 1579, 1716, 2316
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 6 cut(s) 174, 313, 742, 1328, 1446, 2400
TaiI ACGT 4 cut(s) 490, 604, 1476, 1480
TaqI TCGA 8 cut(s) 66, 75, 458, 645, 863, 1092, 1189, 1756
TatI WGTACW 3 cut(s) 174, 266, 2169
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 7 cut(s) 221, 364, 683, 874, 1599, 1867, 2242
Tru1I TTAA 6 cut(s) 308, 375, 576, 809, 1398, 1790
Tru9I TTAA 6 cut(s) 308, 375, 576, 809, 1398, 1790
TseFI GTSAC 4 cut(s) 290, 736, 908, 1566
TseI GCWGC 3 cut(s) 922, 1516, 1664
Tsp45I GTSAC 4 cut(s) 290, 736, 908, 1566
TspGWI ACGGA 2 cut(s) 149, 180
VneI GTGCAC 2 cut(s) 1632, 2394
VpaK11BI GGWCC 7 cut(s) 210, 230, 472, 562, 748, 1720, 1928
XagI CCTNNNNNAGG 1 cut(s) 2298
XapI RAATTY 4 cut(s) 694, 932, 1338, 2324
XceI RCATGY 1 cut(s) 1714
XcmI CCANNNNNNNNNTGG 1 cut(s) 2072
XmnI GAANNNNTTC 2 cut(s) 14, 1104
XspI CTAG 5 cut(s) 843, 1263, 1859, 2178, 2226
ZrmI AGTACT 2 cut(s) 268, 2171
Zsp2I ATGCAT 1 cut(s) 1380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.