Rh4BG416900

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0010181
Physical Location & Seq
Forward (+)
56439175 .. 56446641
7467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG416900.1

Sequence Viewer

Length: 2025 bp
ATGAGCGGGAACGAGTTCCGCTTCTTCTTGTCCTGCGACATCAATCTCCCACTCACCTTTCGGATCGAGCGGCTTGAAGGAGCTCTGAGCCGGCCCAAGTCCACAACCTCCGATGTTGATACAACTCCGGCGACGGGGGAGAGAAGAGCAGAGCTGTATGTGGAGTGCGCATTGTACATTGATGGCGCTCCGTTTGGCTTGCCCACACGGACAAGACTGGAATCTTCAGGGCCTTCATATTGTTGGAACGAGCTGATCACTCTGAGTACTAAGTATAATGACTTGACTGGTCACTCACAGCTTGCTTTTACTGTTTGGGATGTGTCGTGTGGGAAAGATGATGGGTTGATTGGTGGTGCTACGATTCTTCTCTTTAATAACAAGAAGCAACTCAAGACAGGGAAGCAAAAGCTGAGGCTTTGGAAAGGAAAAGTAGCTGATGGGTCATTTCCTACTTCGACTCCTGGAAAGGTTCCCCGGAATGAACGCGGGGAGTTGGAACGTTTGGAGAAGCTAGTGAATAAGTATGAGAGGGGACAGATCCAATGTGTTGATTGGCTGGACCGCCTTGCATTTAAAGCTATGGAGAGAATAAAGGAACGTGAAAGCTCTAAAAATGGAAGTTTGCATTTGTACCTGGTTGTCGATTTCTCTAGTTTTGAGCATCGTGTTGTTTTCCAGGAATCAGGGGCAAATTTCTTATTACCATCCCCTATAGCTTCCACGAATGAAATTGTCACTGTATGGGACCCAGAGCTGGGAAAGATAAATCCATCAGAGCACAAGCAACTAAAACTTGCAAGGAGTTTAACCCGTGGTATCATTGATAGGGATCTCAAGCCAAGCTCCAATGAGAGAAAGTCGATACAGAGGATTCTAAAGTACCCTCCAACAAGGACATTGAGTGGTGACGAAAGGCAGCTTCTGTGGAAATTTCGTTTCTCATTGATGTCTGAGAAGAGGGCCCTCACAAAGTTCCTCAAATGTGTTGAATGGAGTGATGTTCAGGAAGCAAAACAAGCATTAGAATTGATGGGCAGATGGGAAATGATTGATGTTTGCGATGCGTTGGAGCTGTTATCTCCTGTTTTTGAAAGTGAAGAGGTTCGTGCATATGCTGTCAGTGTTCTTGAAAGAGCTGATGATGAAGAACTCCAATGTTACTTACTTCAGCTGGTTCAGGCTCTTCGATTCGAGCGCTCTGATAAATCCTGTCTTTCTCATTTCCTTGTTCAACGTGCATTACGAAACATTGAGTTGGCTAGCTTTCTTCGCTGGTATGTTGCTGTGGAACTTCACGATCCAGCATATGCGAAGCGTTTTTACTGTACCTACGAACTCCTAGAAGAGAATATGATGAAGTTGTCAGCTGGTATGCATGGAGACGAGGATGGATTTAAATTGTGGCAAAGTTTGGTGCGTCAGACAGAATTGACTGCACAACTGTGTTCAATTACAAGAGATGTTAGAAATGTACGTGGCAATACTCAGAAGAAAATTGAAAAGCTTAGACAGCTGCTTTCTGGTCTTCTTAGTGAACTTACATATTTTGAAGAGCCGATTAGATCACCACTGGCTCCAGGAGTCCTCATCAATGGGATTGTGCCATCGGAGTCATCAATATTCAAAAGTGCACTGCATCCTCTTCGCTTGACTTTCCGAGCAGCAAATGGGGGCAGTTGCAAAGTTATCTTCAAGAAGGGGGATGACATTCGCCAGGACCAATTAGTTGTTCAAATGGTATCTCTCATGGATCGATTGCTCAAAAACGAAAATCTTGATCTGCACTTAACTCCTTATAAGGTTCTTGCAACTGGGCAAGATGAAGGAATGATGGAATTCATTCCATCCCGCACGTTAGCGCTGATTCTCTCAGAGCATAGAAGTATTACGAGCTACCTGCAGAAGTTTCATCCAGATGAGCATGGACCATTTGGTATTACTGCCACCTGTCTGGAAACATTTATAAAAAGCTGCGCTGGCTACTCTGTTATCACATATATACTGGGCATTGGAGACAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000407 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005777 GO:0005942 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006914 GO:0006950 GO:0006970 GO:0006996 GO:0007033 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009056 GO:0009058 GO:0009555 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016236 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019637 GO:0019898 GO:0022607 GO:0023052 GO:0030242 GO:0030258 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0034271 GO:0034272 GO:0035004 GO:0035032 GO:0035556 GO:0036092 GO:0036211 GO:0042579 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045017 GO:0046474 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0052742 GO:0055046 GO:0061695 GO:0061919 GO:0065007 GO:0070925 GO:0071704 GO:0071840 GO:0072593 GO:0090407 GO:0097708 GO:0098657 GO:0098796 GO:1901564 GO:1901576 GO:1902494 GO:1905037 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 ko04136 ko04138 ko04140 ko04145 ko04371 ko05152 ko05167 map00562 map01100 map04070 map04136 map04138 map04140 map04145 map04371 map05152 map05167
Pfam Domains
Protein Families
PI3K_C2 PI3Ka PI3_PI4_kinase

Protein Analysis

674

Amino Acids

76.74

Weight (kDa)

7.84

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3K_C2 PF00792 50 - 192 5.8e-42 Phosphoinositide 3-kinase C2
PI3Ka PF00613 278 - 454 6.7e-67 Phosphoinositide 3-kinase family, accessory domain (PIK domain)
PI3_PI4_kinase PF00454 561 - 674 1.4e-25 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1800, 1967
Acc16I TGCGCA 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 1908
AccBSI CCGCTC 2 cut(s) 6, 70
AccII CGCG 1 cut(s) 489
AciI CCGC 6 cut(s) 6, 19, 70, 489, 565, 1852
AclI AACGTT 1 cut(s) 502
AclWI GGATC 5 cut(s) 71, 535, 840, 1295, 1761
AcsI RAATTY 3 cut(s) 694, 932, 1838
AcuI CTGAAG 2 cut(s) 210, 1154
AfaI GTAC 6 cut(s) 176, 268, 635, 884, 1330, 1476
AfeI AGCGCT 2 cut(s) 1199, 1863
AfiI CCNNNNNNNGG 3 cut(s) 134, 757, 758
AjnI CCWGG 5 cut(s) 463, 636, 678, 1579, 1716
AjuI GAANNNNNNNTTGG 2 cut(s) 1716, 1748
AleI CACNNNNGTG 1 cut(s) 1444
Alw21I GWGCWC 3 cut(s) 85, 783, 1636
Alw26I GTCTC 2 cut(s) 1377, 2010
Alw44I GTGCAC 1 cut(s) 1632
AlwI GGATC 5 cut(s) 71, 535, 840, 1295, 1761
AlwNI CAGNNNCTG 1 cut(s) 925
Aor51HI AGCGCT 2 cut(s) 1199, 1863
AoxI GGCC 3 cut(s) 92, 230, 963
ApaI GGGCCC 1 cut(s) 967
ApaLI GTGCAC 1 cut(s) 1632
ApeKI GCWGC 4 cut(s) 919, 1516, 1664, 1974
ApoI RAATTY 3 cut(s) 694, 932, 1838
Asp700I GAANNNNTTC 3 cut(s) 14, 1104, 1842
AspLEI GCGC 5 cut(s) 170, 188, 1200, 1864, 1979
AspS9I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
AsuC2I CCSGG 1 cut(s) 478
AsuHPI GGTGA 3 cut(s) 46, 920, 1560
AsuNHI GCTAGC 1 cut(s) 1262
AvaII GGWCC 4 cut(s) 562, 748, 1720, 1928
BaeGI GKGCMC 2 cut(s) 967, 1636
BanII GRGCYC 2 cut(s) 85, 967
BarI GAAGNNNNNNTAC 2 cut(s) 1307, 1339
BbsI GAAGAC 1 cut(s) 1520
Bbv12I GWGCWC 3 cut(s) 85, 783, 1636
BbvCI CCTCAGC 1 cut(s) 413
BbvI GCAGC 4 cut(s) 931, 1503, 1676, 1961
BcgI CGANNNNNNTGC 4 cut(s) 1628, 1662, 1882, 1916
BciT130I CCWGG 5 cut(s) 465, 638, 680, 1581, 1718
BclI TGATCA 1 cut(s) 255
BcnI CCSGG 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 1377, 2010
BfaI CTAG 4 cut(s) 515, 654, 1263, 1343
BfmI CTRYAG 2 cut(s) 714, 1901
BfoI RGCGCY 3 cut(s) 189, 1201, 1865
BfuAI ACCTGC 1 cut(s) 1908
BisI GCNGC 5 cut(s) 71, 920, 1517, 1665, 1975
BlsI GCNGC 5 cut(s) 72, 921, 1518, 1666, 1976
BmcAI AGTACT 1 cut(s) 268
Bme1390I CCNGG 6 cut(s) 465, 478, 638, 680, 1581, 1718
Bme18I GGWCC 4 cut(s) 562, 748, 1720, 1928
BmgT120I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
BmiI GGNNCC 5 cut(s) 474, 749, 750, 965, 1578
BmrFI CCNGG 6 cut(s) 465, 478, 638, 680, 1581, 1718
BmrI ACTGGG 2 cut(s) 1824, 2015
BmsI GCATC 3 cut(s) 673, 1054, 1648
BmtI GCTAGC 1 cut(s) 1266
BmuI ACTGGG 2 cut(s) 1824, 2015
BpiI GAAGAC 1 cut(s) 1520
BpmI CTGGAG 1 cut(s) 1563
Bpu10I CCTNAGC 1 cut(s) 413
BpuEI CTTGAG 2 cut(s) 377, 821
BpuMI CCSGG 1 cut(s) 478
Bsa29I ATCGAT 1 cut(s) 1756
BsaAI YACGTR 1 cut(s) 1478
BsaBI GATNNNNATC 1 cut(s) 831
BsaJI CCNNGG 2 cut(s) 476, 814
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 757, 758
Bse118I RCCGGY 1 cut(s) 90
Bse1I ACTGG 5 cut(s) 222, 292, 1578, 1819, 2010
Bse8I GATNNNNATC 1 cut(s) 831
BseBI CCWGG 5 cut(s) 465, 638, 680, 1581, 1718
BseCI ATCGAT 1 cut(s) 1756
BseDI CCNNGG 2 cut(s) 476, 814
BseGI GGATG 7 cut(s) 325, 707, 1396, 1639, 1711, 1847, 1912
BseJI GATNNNNATC 1 cut(s) 831
BseLI CCNNNNNNNGG 3 cut(s) 134, 757, 758
BseMII CTCAG 6 cut(s) 77, 254, 404, 945, 1502, 1887
BseNI ACTGG 5 cut(s) 222, 292, 1578, 1819, 2010
BseSI GKGCMC 2 cut(s) 967, 1636
BseXI GCAGC 4 cut(s) 931, 1503, 1676, 1961
BseYI CCCAGC 1 cut(s) 757
BsgI GTGCAG 2 cut(s) 1422, 1769
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 3 cut(s) 94, 232, 965
BshVI ATCGAT 1 cut(s) 1756
BsiHKAI GWGCWC 3 cut(s) 85, 783, 1636
BsiSI CCGG 3 cut(s) 91, 128, 478
BslFI GGGAC 2 cut(s) 549, 761
BslI CCNNNNNNNGG 3 cut(s) 134, 757, 758
BsmAI GTCTC 2 cut(s) 1377, 2010
BsmBI CGTCTC 1 cut(s) 1377
BsmFI GGGAC 2 cut(s) 549, 761
BsnI GGCC 3 cut(s) 94, 232, 965
Bsp120I GGGCCC 1 cut(s) 963
Bsp1286I GDGCHC 4 cut(s) 85, 783, 967, 1636
Bsp1407I TGTACA 1 cut(s) 174
Bsp143I GATC 8 cut(s) 63, 255, 540, 832, 1300, 1565, 1753, 1780
BspACI CCGC 6 cut(s) 6, 19, 70, 489, 565, 1852
BspANI GGCC 3 cut(s) 94, 232, 965
BspCNI CTCAG 6 cut(s) 78, 255, 405, 946, 1501, 1886
BspDI ATCGAT 1 cut(s) 1756
BspFNI CGCG 1 cut(s) 489
BspLI GGNNCC 5 cut(s) 474, 749, 750, 965, 1578
BspMAI CTGCAG 1 cut(s) 1905
BspMI ACCTGC 1 cut(s) 1908
BspOI GCTAGC 1 cut(s) 1266
BspPI GGATC 5 cut(s) 71, 535, 840, 1295, 1761
BspQI GCTCTTC 3 cut(s) 139, 1191, 1548
BsrBI CCGCTC 2 cut(s) 6, 70
BsrFI RCCGGY 1 cut(s) 90
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 5 cut(s) 222, 292, 1578, 1819, 2010
BssAI RCCGGY 1 cut(s) 90
BssECI CCNNGG 2 cut(s) 476, 814
BssMI GATC 8 cut(s) 63, 255, 540, 832, 1300, 1565, 1753, 1780
Bst2UI CCWGG 5 cut(s) 465, 638, 680, 1581, 1718
Bst4CI ACNGT 4 cut(s) 313, 742, 1328, 1446
Bst6I CTCTTC 7 cut(s) 139, 953, 1095, 1191, 1341, 1548, 1650
BstAUI TGTACA 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 1478
BstC8I GCNNGC 5 cut(s) 92, 200, 303, 1264, 1981
BstDEI CTNAG 9 cut(s) 86, 263, 270, 413, 954, 1488, 1508, 1532, 1873
BstDSI CCRYGG 1 cut(s) 814
BstF5I GGATG 7 cut(s) 325, 707, 1396, 1639, 1711, 1847, 1912
BstFNI CGCG 1 cut(s) 489
BstH2I RGCGCY 3 cut(s) 189, 1201, 1865
BstHHI GCGC 5 cut(s) 170, 188, 1200, 1864, 1979
BstKTI GATC 8 cut(s) 66, 258, 543, 835, 1303, 1568, 1756, 1783
BstMAI GTCTC 2 cut(s) 1377, 2010
BstMBI GATC 8 cut(s) 63, 255, 540, 832, 1300, 1565, 1753, 1780
BstMWI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
BstNI CCWGG 5 cut(s) 465, 638, 680, 1581, 1718
BstSCI CCNGG 6 cut(s) 463, 476, 636, 678, 1579, 1716
BstSFI CTRYAG 2 cut(s) 714, 1901
BstSLI GKGCMC 2 cut(s) 967, 1636
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 4 cut(s) 931, 1503, 1676, 1961
BstV2I GAAGAC 1 cut(s) 1520
BstX2I RGATCY 2 cut(s) 540, 832
BstXI CCANNNNNNTGG 1 cut(s) 1954
BstYI RGATCY 2 cut(s) 540, 832
Bsu15I ATCGAT 1 cut(s) 1756
BsuRI GGCC 3 cut(s) 94, 232, 965
BsuTUI ATCGAT 1 cut(s) 1756
BtgI CCRYGG 1 cut(s) 814
BtgZI GCGATG 1 cut(s) 1077
BtsCI GGATG 7 cut(s) 325, 707, 1396, 1639, 1711, 1847, 1912
BtsI GCAGTG 1 cut(s) 1634
BtsIMutI CAGTG 4 cut(s) 738, 1129, 1571, 1634
BveI ACCTGC 1 cut(s) 1908
Cac8I GCNNGC 5 cut(s) 92, 200, 303, 1264, 1981
CaiI CAGNNNCTG 1 cut(s) 925
CfoI GCGC 5 cut(s) 170, 188, 1200, 1864, 1979
Cfr10I RCCGGY 1 cut(s) 90
Cfr13I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
ClaI ATCGAT 1 cut(s) 1756
CseI GACGC 1 cut(s) 1409
CsiI ACCWGGT 1 cut(s) 636
Csp6I GTAC 6 cut(s) 175, 267, 634, 883, 1329, 1475
CviAII CATG 3 cut(s) 1379, 1750, 1925
CviQI GTAC 6 cut(s) 175, 267, 634, 883, 1329, 1475
DdeI CTNAG 9 cut(s) 86, 263, 270, 413, 954, 1488, 1508, 1532, 1873
DpnI GATC 8 cut(s) 65, 257, 542, 834, 1302, 1567, 1755, 1782
DpnII GATC 8 cut(s) 63, 255, 540, 832, 1300, 1565, 1753, 1780
DraI TTTAAA 2 cut(s) 577, 1399
Eam1104I CTCTTC 7 cut(s) 139, 953, 1095, 1191, 1341, 1548, 1650
EarI CTCTTC 7 cut(s) 139, 953, 1095, 1191, 1341, 1548, 1650
Ecl136II GAGCTC 1 cut(s) 83
Eco24I GRGCYC 2 cut(s) 85, 967
Eco47I GGWCC 4 cut(s) 562, 748, 1720, 1928
Eco47III AGCGCT 2 cut(s) 1199, 1863
Eco53kI GAGCTC 1 cut(s) 83
Eco57I CTGAAG 2 cut(s) 210, 1154
EcoICRI GAGCTC 1 cut(s) 83
EcoO109I RGGNCCY 4 cut(s) 230, 748, 963, 964
EcoRI GAATTC 1 cut(s) 1838
EcoRII CCWGG 5 cut(s) 463, 636, 678, 1579, 1716
EcoT22I ATGCAT 1 cut(s) 1380
EcoT38I GRGCYC 2 cut(s) 85, 967
Esp3I CGTCTC 1 cut(s) 1377
FaeI CATG 3 cut(s) 1382, 1753, 1928
FaqI GGGAC 2 cut(s) 549, 761
FatI CATG 3 cut(s) 1378, 1749, 1924
FauI CCCGC 2 cut(s) 482, 1859
FauNDI CATATG 2 cut(s) 1114, 1309
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 5 cut(s) 71, 920, 1517, 1665, 1975
FokI GGATG 7 cut(s) 332, 694, 1403, 1626, 1718, 1834, 1899
FriOI GRGCYC 2 cut(s) 85, 967
Fsp4HI GCNGC 5 cut(s) 71, 920, 1517, 1665, 1975
FspAI RTGCGCAY 1 cut(s) 169
FspBI CTAG 4 cut(s) 515, 654, 1263, 1343
FspI TGCGCA 1 cut(s) 169
GlaI GCGC 5 cut(s) 169, 187, 1199, 1863, 1978
GluI GCNGC 5 cut(s) 71, 920, 1517, 1665, 1975
GsaI CCCAGC 1 cut(s) 761
GsuI CTGGAG 1 cut(s) 1563
HaeII RGCGCY 3 cut(s) 189, 1201, 1865
HaeIII GGCC 3 cut(s) 94, 232, 965
HapII CCGG 3 cut(s) 91, 128, 478
HgaI GACGC 1 cut(s) 1409
HhaI GCGC 5 cut(s) 170, 188, 1200, 1864, 1979
Hin1II CATG 3 cut(s) 1382, 1753, 1928
Hin6I GCGC 5 cut(s) 168, 186, 1198, 1862, 1977
HinP1I GCGC 5 cut(s) 168, 186, 1198, 1862, 1977
HindIII AAGCTT 1 cut(s) 1505
HinfI GANTC 9 cut(s) 221, 364, 460, 683, 874, 1191, 1584, 1613, 1867
HpaII CCGG 3 cut(s) 91, 128, 478
HphI GGTGA 3 cut(s) 46, 920, 1560
Hpy166II GTNNAC 3 cut(s) 102, 1538, 1634
Hpy188III TCNNGA 8 cut(s) 394, 1007, 1130, 1298, 1696, 1778, 1916, 1955
Hpy8I GTNNAC 3 cut(s) 102, 1538, 1634
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 4 cut(s) 71, 243, 1693, 1820
HpyCH4III ACNGT 4 cut(s) 313, 742, 1328, 1446
HpyCH4IV ACGT 5 cut(s) 502, 601, 1237, 1477, 1856
HpyF10VI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
HpyF3I CTNAG 9 cut(s) 86, 263, 270, 413, 954, 1488, 1508, 1532, 1873
HpySE526I ACGT 5 cut(s) 502, 601, 1237, 1477, 1856
Hsp92II CATG 3 cut(s) 1382, 1753, 1928
HspAI GCGC 5 cut(s) 168, 186, 1198, 1862, 1977
KflI GGGWCCC 1 cut(s) 748
KroI GCCGGC 1 cut(s) 90
KroNI GCCGGC 1 cut(s) 92
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 8 cut(s) 63, 255, 540, 832, 1300, 1565, 1753, 1780
LguI GCTCTTC 3 cut(s) 139, 1191, 1548
LmnI GCTCC 5 cut(s) 80, 193, 851, 1072, 1582
Lsp1109I GCAGC 4 cut(s) 931, 1503, 1676, 1961
LweI GCATC 3 cut(s) 673, 1054, 1648
MabI ACCWGGT 1 cut(s) 636
MaeI CTAG 4 cut(s) 515, 654, 1263, 1343
MaeII ACGT 5 cut(s) 502, 601, 1237, 1477, 1856
MaeIII GTNAC 4 cut(s) 290, 736, 908, 1160
MalI GATC 8 cut(s) 65, 257, 542, 834, 1302, 1567, 1755, 1782
MbiI CCGCTC 2 cut(s) 6, 70
MboI GATC 8 cut(s) 63, 255, 540, 832, 1300, 1565, 1753, 1780
MflI RGATCY 2 cut(s) 540, 832
MhlI GDGCHC 4 cut(s) 85, 783, 967, 1636
MlyI GAGTC 3 cut(s) 454, 1593, 1622
MmeI TCCRAC 4 cut(s) 224, 477, 914, 1050
Mph1103I ATGCAT 1 cut(s) 1380
MroNI GCCGGC 1 cut(s) 90
MroXI GAANNNNTTC 3 cut(s) 14, 1104, 1842
MseI TTAA 5 cut(s) 375, 576, 809, 1398, 1790
MslI CAYNNNNRTG 2 cut(s) 1444, 1917
MspA1I CMGCKG 3 cut(s) 1174, 1370, 1516
MspI CCGG 3 cut(s) 91, 128, 478
MspR9I CCNGG 6 cut(s) 465, 478, 638, 680, 1581, 1718
MvaI CCWGG 5 cut(s) 465, 638, 680, 1581, 1718
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
NaeI GCCGGC 1 cut(s) 92
NciI CCSGG 1 cut(s) 478
NdeI CATATG 2 cut(s) 1114, 1309
NdeII GATC 8 cut(s) 63, 255, 540, 832, 1300, 1565, 1753, 1780
NgoMIV GCCGGC 1 cut(s) 90
NheI GCTAGC 1 cut(s) 1262
NlaIII CATG 3 cut(s) 1382, 1753, 1928
NlaIV GGNNCC 5 cut(s) 474, 749, 750, 965, 1578
NmuCI GTSAC 3 cut(s) 290, 736, 908
NsbI TGCGCA 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 1380
OliI CACNNNNGTG 1 cut(s) 1444
PciSI GCTCTTC 3 cut(s) 139, 1191, 1548
PcsI WCGNNNNNNNCGW 1 cut(s) 1243
PdiI GCCGGC 1 cut(s) 92
PdmI GAANNNNTTC 3 cut(s) 14, 1104, 1842
PfeI GAWTC 6 cut(s) 221, 364, 683, 874, 1191, 1867
PfoI TCCNGGA 3 cut(s) 463, 678, 1579
PkrI GCNGC 5 cut(s) 72, 921, 1518, 1666, 1976
PleI GAGTC 3 cut(s) 454, 1592, 1621
PpsI GAGTC 3 cut(s) 454, 1592, 1621
Ppu21I YACGTR 1 cut(s) 1478
PpuMI RGGWCCY 1 cut(s) 748
PsiI TTATAA 2 cut(s) 1800, 1967
Psp124BI GAGCTC 1 cut(s) 85
Psp1406I AACGTT 1 cut(s) 502
Psp5II RGGWCCY 1 cut(s) 748
Psp6I CCWGG 5 cut(s) 463, 636, 678, 1579, 1716
PspFI CCCAGC 1 cut(s) 757
PspGI CCWGG 5 cut(s) 463, 636, 678, 1579, 1716
PspN4I GGNNCC 5 cut(s) 474, 749, 750, 965, 1578
PspOMI GGGCCC 1 cut(s) 963
PspPI GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
PspPPI RGGWCCY 1 cut(s) 748
PstI CTGCAG 1 cut(s) 1905
PstNI CAGNNNCTG 1 cut(s) 925
PsuI RGATCY 2 cut(s) 540, 832
PvuII CAGCTG 3 cut(s) 1174, 1370, 1516
RsaI GTAC 6 cut(s) 176, 268, 635, 884, 1330, 1476
RsaNI GTAC 6 cut(s) 175, 267, 634, 883, 1329, 1475
RseI CAYNNNNRTG 2 cut(s) 1444, 1917
SacI GAGCTC 1 cut(s) 85
SapI GCTCTTC 3 cut(s) 139, 1191, 1548
SaqAI TTAA 5 cut(s) 375, 576, 809, 1398, 1790
SatI GCNGC 5 cut(s) 71, 920, 1517, 1665, 1975
Sau3AI GATC 8 cut(s) 63, 255, 540, 832, 1300, 1565, 1753, 1780
Sau96I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
ScaI AGTACT 1 cut(s) 268
SchI GAGTC 3 cut(s) 454, 1593, 1622
ScrFI CCNGG 6 cut(s) 465, 478, 638, 680, 1581, 1718
SduI GDGCHC 4 cut(s) 85, 783, 967, 1636
SexAI ACCWGGT 1 cut(s) 636
SfaNI GCATC 3 cut(s) 673, 1054, 1648
SfcI CTRYAG 2 cut(s) 714, 1901
SinI GGWCC 4 cut(s) 562, 748, 1720, 1928
SmiI ATTTAAAT 1 cut(s) 1399
SmiMI CAYNNNNRTG 2 cut(s) 1444, 1917
SmlI CTYRAG 2 cut(s) 392, 836
SmoI CTYRAG 2 cut(s) 392, 836
SsiI CCGC 6 cut(s) 6, 19, 70, 489, 565, 1852
SspI AATATT 1 cut(s) 1623
SspMI CTAG 4 cut(s) 515, 654, 1263, 1343
SstI GAGCTC 1 cut(s) 85
StyD4I CCNGG 6 cut(s) 463, 476, 636, 678, 1579, 1716
SwaI ATTTAAAT 1 cut(s) 1399
TaaI ACNGT 4 cut(s) 313, 742, 1328, 1446
TaiI ACGT 5 cut(s) 505, 604, 1240, 1480, 1859
TaqI TCGA 7 cut(s) 66, 458, 645, 863, 1189, 1194, 1756
TatI WGTACW 2 cut(s) 174, 266
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 6 cut(s) 221, 364, 683, 874, 1191, 1867
Tru1I TTAA 5 cut(s) 375, 576, 809, 1398, 1790
Tru9I TTAA 5 cut(s) 375, 576, 809, 1398, 1790
TscAI CASTG 4 cut(s) 745, 1129, 1578, 1641
TseFI GTSAC 3 cut(s) 290, 736, 908
TseI GCWGC 4 cut(s) 919, 1516, 1664, 1974
Tsp45I GTSAC 3 cut(s) 290, 736, 908
TspDTI ATGAA 8 cut(s) 225, 498, 744, 1161, 1373, 1831, 1839, 1901
TspGWI ACGGA 2 cut(s) 180, 223
TspRI CASTG 4 cut(s) 745, 1129, 1578, 1641
VneI GTGCAC 1 cut(s) 1632
VpaK11BI GGWCC 4 cut(s) 562, 748, 1720, 1928
XapI RAATTY 3 cut(s) 694, 932, 1838
XmnI GAANNNNTTC 3 cut(s) 14, 1104, 1842
XspI CTAG 4 cut(s) 515, 654, 1263, 1343
ZrmI AGTACT 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 1380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.