MD16G1095200.v1.1

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010181
Physical Location & Seq
Reverse (-)
6583307 .. 6589985
6679 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1095200.v1.1.491

Sequence Viewer

Length: 2451 bp
ATGAGCGGGAACGAGTTCCGCTTCTTCTTGTCCTGCGACATCAACCTCCCAGTAACCTTCCGGATCGAACTGCTAGAAGGAGCCTTGCCTCCCTCCAAGTCCCCTAGCTCCGATGTTGATCCCGCTTTCGCAACCGAAGAGAGAAATTCAGAGCTGTATGTGGAGTGTGCATTGTACATTGATGGTGCTCCATTTGGGCTTCCCACCAGGACAAGGCTGGAGTCTTCAGGACCATTATATTGTTGGAACGAACTCGTTACTCTGAGTACGAAATACCGAGACTTAACCGGTCACTCACAACTTGCTCTAACAGTTTGGGATGTTTCGTGTGGGAAGGATGAAGGGTTGGTTGGTGGTGCCACAATTCTTCTTTTTAATAGCAAAAAGCAACTCAAAACAGGGAAGCAAAAGCTGAGGCTTTGGAAGGGAAAAGTAGCAGACGGGTCGTTTCCTACTTCTACTCCTGGAAAGGTCCCCAGGCATGAGCGTGGGGAGTTGGAACGCTTGGAGAAGCTCGTGAATAGGTATGAGAGGGGGCAGATCCAATGTGTTGATTGGCTGGACCGCCTGGCTTTTAAAGCTATGGAGAGAATAAAGGAACGGGAAAGCTCTAAAAATGGAAGTTTGCATTTGTACCTGGTTGTCGATTTCTGTAGTTTTGAACATCGTGTTGCTTTCCAGGAATCAGGGGCAAATTTCTTGTTACCGTCCCCAATAGCTTCAACAAATGAAATTGTCACGGTCTGGGACCCAGAAGTGGGAAAGATAAATCCATCAGAGCACAAGCAACTAAAACTCGCAAGGAGTTTAACCCGTGGTATCATTGACTGGGATCTCAAACCAAGCTCCAACGAAAGAAAGTCGATACAGAGGATTTTGAAATACCCACCAACAAGGACTTTGACTGGCGATGAAAGGCAGCTCCTCTGGAAATTTCGTTTCTCATTGATGTCAGAAAAGAGGGCCCTCACAAAGTTCCTCAGATGTGTTGAATGGAGTGATATTCAGGAAGCAAAACAAGCATTAGAACTGATGGCCAAATGGGACATGATTGATGTCTGTGACGCATTAGAGCTGCTATCTCCTGTTTTTGAAAGTGAAGAGGTTCGTGCATATGCTGTCAGTGTTCTTGAAAGAGCTGATGATGAAGAGCTCCAGTGTTACTTACTTCAACTGGTTCAGGCTCTTCGTTTTGAGCGTTCTGATAAATCTCGTCTTTCTCATTTCCTTGTGCAACGTGCGTTAAGAAACATTGAGTTGGCTAGCTTTCTACGCTGGTATGTTGCTGTAGAACTCCATGATCCAGCATATGCCAAACGGTTTTACTGTACCTACGAAATCCTGGAAGAGAATATGATGAAGTTGCCAGCTGGTGTGCAAGGAGATGAAGATGGATTTAAACTGTGGCAAAGTTTGGTGCGCCAGACAGAATTAACTGCACAGTTGTGTTCAATAACGAGAGATGTAAGAAATGTACGTGGCAATACCCAGAAGAAGATTGAAAAGCTTAGACAACTGCTTTCTGGTCTCCTTAGTGAACTTACGTATTTTGAAGAGCCGATAAGATCACCGCTGGCTCCAGGAGTCCTCATAACTGGGATTGTGCCATCTGAGTCATCAATATTCAAAAGTGCACTGCATCCTTTGCGTTTGACTTTCCGAACAGCAAGTGGGGGAAGTTGCAAAGTTATCTTTAAGAAGGGGGATGACATTCGCCAAGACCAATTAGTTGTTCAAATGGTATATCTCATGGATCGATTGCTGAAATTGGAAAATCTTGATCTGCACTTAACTCCTTATCGAGTTCTTGCGACTGGGCAAGATGAAGGAATGCTAAAATTGATACCATCCCGCTCATTAGCACAGATTCTCTCAGAGCACAGAAGCATTACAAGCTACCTGCAGAAATTTCATCCAGATGAGCATGGACCATTTGGTATTACTGCTACCTGTCTGGAAACATTTATAAAGAGCTGTGCTGGCTACTCTGTTATCACATATATACTGGGCATTGGAGACAGGCATCTTGACAACCTTCTCCTTGTGGACGATGGACGTCTTTTTCATGTTGATTTTGGCTTCATTTTGGGTCGTGATCCAAAGCCATTTCCGCCGCCAATGAAGCTTTGCAAAGAAATGGTTGAGGCTATGGGTGGAGCTGAAAGCCAGTACTATACAAGGTTCAAATCCTACTGTTGCGAAGCGTACAACATTATTAGGAAATCCAGTAACCTAATCTTGAACCTGTTTCACCTAATGGCGGGTTCCAACATTCCTGACATAGCTTCAGATCCTGAAAAAGGCATCCTCAAGCTCCAGGAGAAGTTCCGGTTGGACTTGGACGATGAAGCCTCCATACATTTCTTCCAGGATTTAATCAATGAGAGTGTCAGTGCATTGTTTCCTCAAATGGTCGAGACCATTCATCGGTGGGCTCAATACTGGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000407 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005777 GO:0005942 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006914 GO:0006950 GO:0006970 GO:0006996 GO:0007033 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009056 GO:0009058 GO:0009555 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016236 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019637 GO:0019898 GO:0022607 GO:0023052 GO:0030242 GO:0030258 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0034271 GO:0034272 GO:0035004 GO:0035032 GO:0035556 GO:0036092 GO:0036211 GO:0042579 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045017 GO:0046474 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0052742 GO:0055046 GO:0061695 GO:0061919 GO:0065007 GO:0070925 GO:0071704 GO:0071840 GO:0072593 GO:0090407 GO:0097708 GO:0098657 GO:0098796 GO:1901564 GO:1901576 GO:1902494 GO:1905037 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 ko04136 ko04138 ko04140 ko04145 ko04371 ko05152 ko05167 map00562 map01100 map04070 map04136 map04138 map04140 map04145 map04371 map05152 map05167
Pfam Domains
Protein Families
PI3K_C2 PI3Ka PI3_PI4_kinase

Protein Analysis

817

Amino Acids

93.27

Weight (kDa)

6.54

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3K_C2 PF00792 49 - 192 1e-41 Phosphoinositide 3-kinase C2
PI3Ka PF00613 278 - 454 2.6e-66 Phosphoinositide 3-kinase family, accessory domain (PIK domain)
PI3_PI4_kinase PF00454 561 - 762 5.5e-56 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1967
AatII GACGTC 1 cut(s) 2059
Acc36I ACCTGC 1 cut(s) 1908
AccB1I GGYRCC 1 cut(s) 356
AccBSI CCGCTC 2 cut(s) 6, 1854
AccIII TCCGGA 1 cut(s) 60
AciI CCGC 9 cut(s) 6, 19, 123, 565, 1571, 1852, 2111, 2114, 2261
AclWI GGATC 8 cut(s) 71, 113, 535, 840, 1295, 1761, 2090, 2285
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 4 cut(s) 145, 694, 932, 1907
AcuI CTGAAG 2 cut(s) 210, 2271
AcyI GRCGYC 1 cut(s) 2056
AfaI GTAC 7 cut(s) 176, 268, 635, 1330, 1476, 2171, 2207
AfiI CCNNNNNNNGG 6 cut(s) 213, 757, 758, 2260, 2300, 2427
AgeI ACCGGT 1 cut(s) 287
AjuI GAANNNNNNNTTGG 6 cut(s) 333, 365, 1716, 1748, 2315, 2347
AleI CACNNNNGTG 1 cut(s) 1444
Alw21I GWGCWC 5 cut(s) 190, 783, 1155, 1636, 1881
Alw26I GTCTC 4 cut(s) 273, 1532, 2010, 2411
Alw44I GTGCAC 1 cut(s) 1632
AlwI GGATC 8 cut(s) 71, 113, 535, 840, 1295, 1761, 2090, 2285
AlwNI CAGNNNCTG 1 cut(s) 2294
Aor13HI TCCGGA 1 cut(s) 60
AoxI GGCC 2 cut(s) 963, 1035
ApaI GGGCCC 1 cut(s) 967
ApaLI GTGCAC 1 cut(s) 1632
ApeKI GCWGC 2 cut(s) 919, 1075
ApoI RAATTY 4 cut(s) 145, 694, 932, 1907
AsiGI ACCGGT 1 cut(s) 287
Asp700I GAANNNNTTC 2 cut(s) 14, 1104
AspLEI GCGC 2 cut(s) 1422, 2448
AspS9I GGNCC 7 cut(s) 230, 472, 562, 748, 963, 964, 1928
AsuHPI GGTGA 2 cut(s) 1560, 2243
AsuNHI GCTAGC 1 cut(s) 1262
AvaII GGWCC 5 cut(s) 230, 472, 562, 748, 1928
BaeGI GKGCMC 2 cut(s) 967, 1636
BalI TGGCCA 1 cut(s) 1037
BanI GGYRCC 1 cut(s) 356
BanII GRGCYC 3 cut(s) 967, 1155, 2437
BarI GAAGNNNNNNTAC 2 cut(s) 2340, 2372
BauI CACGAG 1 cut(s) 515
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 5 cut(s) 190, 783, 1155, 1636, 1881
BbvCI CCTCAGC 1 cut(s) 413
BbvI GCAGC 2 cut(s) 931, 1062
BccI CCATC 7 cut(s) 176, 781, 1027, 1385, 1615, 1855, 2045
BcgI CGANNNNNNTGC 2 cut(s) 426, 460
BcoDI GTCTC 4 cut(s) 273, 1532, 2010, 2411
BfaI CTAG 3 cut(s) 74, 105, 1263
BfmI CTRYAG 3 cut(s) 652, 1287, 1901
BfoI RGCGCY 1 cut(s) 2449
BfuAI ACCTGC 1 cut(s) 1908
BisI GCNGC 3 cut(s) 920, 1076, 2114
BlsI GCNGC 3 cut(s) 921, 1077, 2115
BmcAI AGTACT 1 cut(s) 2171
Bme18I GGWCC 5 cut(s) 230, 472, 562, 748, 1928
BmgT120I GGNCC 7 cut(s) 230, 472, 562, 748, 963, 964, 1928
BmiI GGNNCC 8 cut(s) 82, 358, 474, 749, 750, 965, 1578, 2266
BmrI ACTGGG 5 cut(s) 44, 838, 1605, 1824, 2015
BmsI GCATC 3 cut(s) 1648, 2032, 2313
BmtI GCTAGC 1 cut(s) 1266
BmuI ACTGGG 5 cut(s) 44, 838, 1605, 1824, 2015
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 4 cut(s) 239, 1139, 1563, 2300
Bpu10I CCTNAGC 1 cut(s) 413
BpuEI CTTGAG 1 cut(s) 2294
Bsa29I ATCGAT 1 cut(s) 1756
BsaAI YACGTR 2 cut(s) 1478, 1545
BsaBI GATNNNNATC 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 2056
BsaI GGTCTC 2 cut(s) 1532, 2411
BsaJI CCNNGG 2 cut(s) 476, 814
BsaWI WCCGGW 3 cut(s) 60, 287, 2328
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 6 cut(s) 213, 757, 758, 2260, 2300, 2427
Bse118I RCCGGY 1 cut(s) 287
Bse8I GATNNNNATC 1 cut(s) 117
BseAI TCCGGA 1 cut(s) 60
BseCI ATCGAT 1 cut(s) 1756
BseDI CCNNGG 2 cut(s) 476, 814
BseGI GGATG 7 cut(s) 325, 343, 1639, 1711, 1847, 1912, 2304
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 6 cut(s) 213, 757, 758, 2260, 2300, 2427
BseMII CTCAG 5 cut(s) 254, 404, 994, 1602, 1887
BseRI GAGGAG 1 cut(s) 914
BseSI GKGCMC 2 cut(s) 967, 1636
BseXI GCAGC 2 cut(s) 931, 1062
BsgI GTGCAG 2 cut(s) 1422, 1769
BshFI GGCC 2 cut(s) 965, 1037
BshNI GGYRCC 1 cut(s) 356
BshTI ACCGGT 1 cut(s) 287
BshVI ATCGAT 1 cut(s) 1756
BsiHKAI GWGCWC 5 cut(s) 190, 783, 1155, 1636, 1881
BsiSI CCGG 3 cut(s) 61, 288, 2329
BslFI GGGAC 5 cut(s) 85, 458, 694, 761, 1058
BslI CCNNNNNNNGG 6 cut(s) 213, 757, 758, 2260, 2300, 2427
BsmAI GTCTC 4 cut(s) 273, 1532, 2010, 2411
BsmFI GGGAC 5 cut(s) 85, 458, 694, 761, 1058
BsmI GAATGC 1 cut(s) 1836
BsnI GGCC 2 cut(s) 965, 1037
Bso31I GGTCTC 2 cut(s) 1532, 2411
Bsp120I GGGCCC 1 cut(s) 963
Bsp1286I GDGCHC 7 cut(s) 190, 783, 967, 1155, 1636, 1881, 2437
Bsp13I TCCGGA 1 cut(s) 60
Bsp1407I TGTACA 1 cut(s) 174
BspACI CCGC 9 cut(s) 6, 19, 123, 565, 1571, 1852, 2111, 2114, 2261
BspANI GGCC 2 cut(s) 965, 1037
BspCNI CTCAG 5 cut(s) 255, 405, 993, 1603, 1886
BspDI ATCGAT 1 cut(s) 1756
BspEI TCCGGA 1 cut(s) 60
BspLI GGNNCC 8 cut(s) 82, 358, 474, 749, 750, 965, 1578, 2266
BspMAI CTGCAG 1 cut(s) 1905
BspMI ACCTGC 1 cut(s) 1908
BspOI GCTAGC 1 cut(s) 1266
BspPI GGATC 8 cut(s) 71, 113, 535, 840, 1295, 1761, 2090, 2285
BspQI GCTCTTC 3 cut(s) 1143, 1191, 1548
BspT107I GGYRCC 1 cut(s) 356
BspTNI GGTCTC 2 cut(s) 1532, 2411
BsrBI CCGCTC 2 cut(s) 6, 1854
BsrFI RCCGGY 1 cut(s) 287
BsrGI TGTACA 1 cut(s) 174
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 2 cut(s) 476, 814
BssNI GRCGYC 1 cut(s) 2056
BssSI CACGAG 1 cut(s) 515
Bst2BI CACGAG 1 cut(s) 515
Bst4CI ACNGT 8 cut(s) 313, 708, 742, 1320, 1328, 1404, 1443, 2195
Bst6I CTCTTC 6 cut(s) 132, 1095, 1143, 1191, 1341, 1548
BstACI GRCGYC 1 cut(s) 2056
BstAPI GCANNNNNTGC 1 cut(s) 1645
BstAUI TGTACA 1 cut(s) 174
BstBAI YACGTR 2 cut(s) 1478, 1545
BstC8I GCNNGC 4 cut(s) 1264, 1368, 1575, 1981
BstDEI CTNAG 7 cut(s) 263, 413, 980, 1508, 1532, 1611, 1873
BstDSI CCRYGG 1 cut(s) 814
BstENI CCTNNNNNAGG 1 cut(s) 2298
BstF5I GGATG 7 cut(s) 325, 343, 1639, 1711, 1847, 1912, 2304
BstH2I RGCGCY 1 cut(s) 2449
BstHHI GCGC 2 cut(s) 1422, 2448
BstMAI GTCTC 4 cut(s) 273, 1532, 2010, 2411
BstMWI GCNNNNNNNGC 8 cut(s) 578, 1019, 1272, 1645, 1893, 1980, 2110, 2122
BstSFI CTRYAG 3 cut(s) 652, 1287, 1901
BstSLI GKGCMC 2 cut(s) 967, 1636
BstSNI TACGTA 1 cut(s) 1545
BstV1I GCAGC 2 cut(s) 931, 1062
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 3 cut(s) 540, 832, 2290
BstYI RGATCY 3 cut(s) 540, 832, 2290
Bsu15I ATCGAT 1 cut(s) 1756
BsuRI GGCC 2 cut(s) 965, 1037
BsuTUI ATCGAT 1 cut(s) 1756
BtgI CCRYGG 1 cut(s) 814
BtgZI GCGATG 1 cut(s) 924
BtsCI GGATG 7 cut(s) 325, 343, 1639, 1711, 1847, 1912, 2304
BtsI GCAGTG 1 cut(s) 1634
BtsIMutI CAGTG 4 cut(s) 1129, 1163, 1634, 2398
BveI ACCTGC 1 cut(s) 1908
Cac8I GCNNGC 4 cut(s) 1264, 1368, 1575, 1981
CaiI CAGNNNCTG 1 cut(s) 2294
CfoI GCGC 2 cut(s) 1422, 2448
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 7 cut(s) 230, 472, 562, 748, 963, 964, 1928
ClaI ATCGAT 1 cut(s) 1756
CseI GACGC 1 cut(s) 1073
CsiI ACCWGGT 1 cut(s) 636
Csp6I GTAC 7 cut(s) 175, 267, 634, 1329, 1475, 2170, 2206
CspAI ACCGGT 1 cut(s) 287
CviAII CATG 6 cut(s) 482, 1048, 1298, 1750, 1925, 2066
CviQI GTAC 7 cut(s) 175, 267, 634, 1329, 1475, 2170, 2206
DdeI CTNAG 7 cut(s) 263, 413, 980, 1508, 1532, 1611, 1873
DraI TTTAAA 2 cut(s) 577, 1399
EaeI YGGCCR 1 cut(s) 1035
Eam1104I CTCTTC 6 cut(s) 132, 1095, 1143, 1191, 1341, 1548
EarI CTCTTC 6 cut(s) 132, 1095, 1143, 1191, 1341, 1548
EciI GGCGGA 1 cut(s) 2100
Ecl136II GAGCTC 1 cut(s) 1153
Eco105I TACGTA 1 cut(s) 1545
Eco24I GRGCYC 3 cut(s) 967, 1155, 2437
Eco31I GGTCTC 2 cut(s) 1532, 2411
Eco47I GGWCC 5 cut(s) 230, 472, 562, 748, 1928
Eco53kI GAGCTC 1 cut(s) 1153
Eco57I CTGAAG 2 cut(s) 210, 2271
EcoICRI GAGCTC 1 cut(s) 1153
EcoNI CCTNNNNNAGG 1 cut(s) 2298
EcoO109I RGGNCCY 4 cut(s) 472, 748, 963, 964
EcoT38I GRGCYC 3 cut(s) 967, 1155, 2437
FaeI CATG 6 cut(s) 485, 1051, 1301, 1753, 1928, 2069
FaqI GGGAC 5 cut(s) 85, 458, 694, 761, 1058
FatI CATG 6 cut(s) 481, 1047, 1297, 1749, 1924, 2065
FauI CCCGC 3 cut(s) 130, 1859, 2254
FauNDI CATATG 2 cut(s) 1114, 1309
Fnu4HI GCNGC 3 cut(s) 920, 1076, 2114
FokI GGATG 7 cut(s) 332, 350, 1626, 1718, 1834, 1899, 2291
FriOI GRGCYC 3 cut(s) 967, 1155, 2437
Fsp4HI GCNGC 3 cut(s) 920, 1076, 2114
FspBI CTAG 3 cut(s) 74, 105, 1263
GlaI GCGC 2 cut(s) 1421, 2447
GluI GCNGC 3 cut(s) 920, 1076, 2114
GsuI CTGGAG 4 cut(s) 239, 1139, 1563, 2300
HaeII RGCGCY 1 cut(s) 2449
HaeIII GGCC 2 cut(s) 965, 1037
HapII CCGG 3 cut(s) 61, 288, 2329
HgaI GACGC 1 cut(s) 1073
HhaI GCGC 2 cut(s) 1422, 2448
Hin1I GRCGYC 1 cut(s) 2056
Hin1II CATG 6 cut(s) 485, 1051, 1301, 1753, 1928, 2069
Hin6I GCGC 2 cut(s) 1420, 2446
HinP1I GCGC 2 cut(s) 1420, 2446
HindIII AAGCTT 2 cut(s) 1505, 2123
HinfI GANTC 5 cut(s) 221, 683, 1584, 1613, 1867
HpaII CCGG 3 cut(s) 61, 288, 2329
HphI GGTGA 2 cut(s) 1560, 2243
Hpy166II GTNNAC 3 cut(s) 1538, 1634, 2047
Hpy8I GTNNAC 3 cut(s) 1538, 1634, 2047
HpyAV CCTTC 8 cut(s) 67, 71, 328, 335, 418, 1693, 1820, 2045
HpyCH4III ACNGT 8 cut(s) 313, 708, 742, 1320, 1328, 1404, 1443, 2195
HpyCH4IV ACGT 4 cut(s) 1237, 1477, 1544, 2056
HpyF10VI GCNNNNNNNGC 8 cut(s) 578, 1019, 1272, 1645, 1893, 1980, 2110, 2122
HpyF3I CTNAG 7 cut(s) 263, 413, 980, 1508, 1532, 1611, 1873
HpySE526I ACGT 4 cut(s) 1237, 1477, 1544, 2056
Hsp92I GRCGYC 1 cut(s) 2056
Hsp92II CATG 6 cut(s) 485, 1051, 1301, 1753, 1928, 2069
HspAI GCGC 2 cut(s) 1420, 2446
KflI GGGWCCC 1 cut(s) 748
Kpn2I TCCGGA 1 cut(s) 60
LguI GCTCTTC 3 cut(s) 1143, 1191, 1548
LmnI GCTCC 9 cut(s) 80, 113, 193, 851, 927, 1158, 1582, 2156, 2319
Lsp1109I GCAGC 2 cut(s) 931, 1062
LweI GCATC 3 cut(s) 1648, 2032, 2313
MabI ACCWGGT 1 cut(s) 636
MaeI CTAG 3 cut(s) 74, 105, 1263
MaeII ACGT 4 cut(s) 1237, 1477, 1544, 2056
MaeIII GTNAC 8 cut(s) 52, 256, 290, 702, 736, 1061, 1160, 2228
MbiI CCGCTC 2 cut(s) 6, 1854
MflI RGATCY 3 cut(s) 540, 832, 2290
MhlI GDGCHC 7 cut(s) 190, 783, 967, 1155, 1636, 1881, 2437
MlsI TGGCCA 1 cut(s) 1037
MluNI TGGCCA 1 cut(s) 1037
MlyI GAGTC 3 cut(s) 230, 1593, 1622
MmeI TCCRAC 5 cut(s) 224, 477, 873, 2292, 2313
Mox20I TGGCCA 1 cut(s) 1037
MroI TCCGGA 1 cut(s) 60
MroXI GAANNNNTTC 2 cut(s) 14, 1104
MscI TGGCCA 1 cut(s) 1037
MslI CAYNNNNRTG 3 cut(s) 486, 1444, 1917
Msp20I TGGCCA 1 cut(s) 1037
MspA1I CMGCKG 2 cut(s) 1370, 1573
MspI CCGG 3 cut(s) 61, 288, 2329
Mva1269I GAATGC 1 cut(s) 1836
MwoI GCNNNNNNNGC 8 cut(s) 578, 1019, 1272, 1645, 1893, 1980, 2110, 2122
NdeI CATATG 2 cut(s) 1114, 1309
NheI GCTAGC 1 cut(s) 1262
NlaIII CATG 6 cut(s) 485, 1051, 1301, 1753, 1928, 2069
NlaIV GGNNCC 8 cut(s) 82, 358, 474, 749, 750, 965, 1578, 2266
NmuCI GTSAC 3 cut(s) 290, 736, 1061
OliI CACNNNNGTG 1 cut(s) 1444
PciSI GCTCTTC 3 cut(s) 1143, 1191, 1548
PcsI WCGNNNNNNNCGW 1 cut(s) 1195
PctI GAATGC 1 cut(s) 1836
PdmI GAANNNNTTC 2 cut(s) 14, 1104
PfeI GAWTC 2 cut(s) 683, 1867
PfoI TCCNGGA 6 cut(s) 463, 678, 1341, 1579, 2316, 2367
PinAI ACCGGT 1 cut(s) 287
PkrI GCNGC 3 cut(s) 921, 1077, 2115
PleI GAGTC 3 cut(s) 229, 1592, 1621
PpsI GAGTC 3 cut(s) 229, 1592, 1621
Ppu21I YACGTR 2 cut(s) 1478, 1545
PpuMI RGGWCCY 2 cut(s) 472, 748
PsiI TTATAA 1 cut(s) 1967
Psp124BI GAGCTC 1 cut(s) 1155
Psp5II RGGWCCY 2 cut(s) 472, 748
PspN4I GGNNCC 8 cut(s) 82, 358, 474, 749, 750, 965, 1578, 2266
PspOMI GGGCCC 1 cut(s) 963
PspPI GGNCC 7 cut(s) 230, 472, 562, 748, 963, 964, 1928
PspPPI RGGWCCY 2 cut(s) 472, 748
PstI CTGCAG 1 cut(s) 1905
PstNI CAGNNNCTG 1 cut(s) 2294
PsuI RGATCY 3 cut(s) 540, 832, 2290
PvuII CAGCTG 1 cut(s) 1370
RsaI GTAC 7 cut(s) 176, 268, 635, 1330, 1476, 2171, 2207
RsaNI GTAC 7 cut(s) 175, 267, 634, 1329, 1475, 2170, 2206
RseI CAYNNNNRTG 3 cut(s) 486, 1444, 1917
SacI GAGCTC 1 cut(s) 1155
SapI GCTCTTC 3 cut(s) 1143, 1191, 1548
SatI GCNGC 3 cut(s) 920, 1076, 2114
Sau96I GGNCC 7 cut(s) 230, 472, 562, 748, 963, 964, 1928
ScaI AGTACT 1 cut(s) 2171
SchI GAGTC 3 cut(s) 230, 1593, 1622
SduI GDGCHC 7 cut(s) 190, 783, 967, 1155, 1636, 1881, 2437
SexAI ACCWGGT 1 cut(s) 636
SfaNI GCATC 3 cut(s) 1648, 2032, 2313
SfcI CTRYAG 3 cut(s) 652, 1287, 1901
SinI GGWCC 5 cut(s) 230, 472, 562, 748, 1928
SmiMI CAYNNNNRTG 3 cut(s) 486, 1444, 1917
SmlI CTYRAG 1 cut(s) 2309
SmoI CTYRAG 1 cut(s) 2309
SnaBI TACGTA 1 cut(s) 1545
SsiI CCGC 9 cut(s) 6, 19, 123, 565, 1571, 1852, 2111, 2114, 2261
SspI AATATT 1 cut(s) 1623
SspMI CTAG 3 cut(s) 74, 105, 1263
SstI GAGCTC 1 cut(s) 1155
TaaI ACNGT 8 cut(s) 313, 708, 742, 1320, 1328, 1404, 1443, 2195
TaiI ACGT 4 cut(s) 1240, 1480, 1547, 2059
TaqI TCGA 6 cut(s) 66, 645, 863, 1756, 1801, 2415
TatI WGTACW 2 cut(s) 174, 2169
TauI GCSGC 1 cut(s) 2116
TfiI GAWTC 2 cut(s) 683, 1867
TscAI CASTG 4 cut(s) 1129, 1163, 1641, 2398
TseFI GTSAC 3 cut(s) 290, 736, 1061
TseI GCWGC 2 cut(s) 919, 1075
Tsp45I GTSAC 3 cut(s) 290, 736, 1061
TspRI CASTG 4 cut(s) 1129, 1163, 1641, 2398
VneI GTGCAC 1 cut(s) 1632
VpaK11BI GGWCC 5 cut(s) 230, 472, 562, 748, 1928
XagI CCTNNNNNAGG 1 cut(s) 2298
XapI RAATTY 4 cut(s) 145, 694, 932, 1907
XcmI CCANNNNNNNNNTGG 2 cut(s) 214, 240
XmnI GAANNNNTTC 2 cut(s) 14, 1104
XspI CTAG 3 cut(s) 74, 105, 1263
ZraI GACGTC 1 cut(s) 2057
ZrmI AGTACT 1 cut(s) 2171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.