Rh4CG431400

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0010181
Physical Location & Seq
Forward (+)
67178089 .. 67185925
7837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG431400.1

Sequence Viewer

Length: 2529 bp
ATGAGCGGGAACGAGTTCCGCTTCTTCTTGTCCTGCGACATCAATCTCCCACTCACCTTTCGGATCGAGCGGCTTGAAGGAGCTCTGAGCCGGCCCAAGTCCACAACCTCCGATGTTGATCCAACTCCGGCGACGGAGGAGAGAAGAGCAGAGCTGTATGTGGAGTGCGCATTGTACATTGATGGCGCTCCGTTTGGCTTGCCCACACGGACAAGGCTGGAATCTTCAGGGCCTTCATATTGTTGGAACGAGCTGATTACTCTGAGTACTAAGTATAATGACTTGACTGGTCACTCACAGCTTGCTTTTACTGTTTGGGATGTTTCGTGTGGGAAAGATGAAGGGTTGATTGGTGGTGCTACGATTCTTCTCTTTAATAACAAGAAGCAACTCAAGACAGGGAAGCAAAAGTTGAGGCTTTGGCCAGGAAAAGTAGCTGATGGGTCATTTCCTACTTCGACTCCTGGAAAGGTTCCCCGGAATGAACGTGGGGAGTTGGAACGTTTGGAGAAGCTAGTGAATAAGTATGAGAGGGGACAGATCCAATGTGTTGATTGGCTGGACCGCCTTGCATTTAAAGCTATGGAGAGAATAAAGGAACGTGAAAGCTCTAAAAATGGAAGTTTGCATTTGTACCTGGTTGTCGATTTCTGTAGTTTTGAGCATCGTGTTGTTTTCCAGGAATCAGGGGCAAATTTCTTATTACCATCCCCTATAGCTTCCACGAATGAAATTGTCACTGTATGGGACCCAGAGCTGGGAAAGATAAATCCATCAGAGCACAAGCAACTAAAACTTGCAAGGAGTTTAACCCGTGGTATCATTGATAGGGATCTCAAGCCAAGCTCCAATGAGAGAAAGTCGATACAGAGGATTCTAAAGTACCCTCCAACAAGGACATTGAGTGGTGACGAAAGGCAGCTTCTGTGGAAATTTCGTTTCTCATTGATGTCTGAGAAGAGGGCCCTCACAAAGTTCCTCAAATGTGTTGAATGGAGTGATGTTCAGGAAGCAAAACAAGCATTAGAATTGATGGGCAGATGGGAAATGATTGATGTTTGCGATGCGTTGGAGCTGTTATCTCCTGTTTTTGAAAGTGAAGAGGTTCGTGCATATGCTGTCAGTGTTCTTGAAAGAGCTGATGATGAAGAACTCCAATGTTACTTACTTCAGCTGGTTCAGGCTCTTCGATTCGAGCGCTCTGATAAATCCTGTCTTTCTCATTTCCTTGTTCAACGTGCATTACGAAACATTGAGTTGGCTAGCTTTCTTCGCTGGTATGTTGCTGTGGAACTTCACGATCCAGCATATGCGAAGCGTTTTTACTGTACCTACGAACTCCTGGAAGAGAATATGATGAAGTTGTCAGCTGGTATGCATGGAGACGAGGATGGATTTAAATTGTGGCAAAGTTTGGTGCGTCAGACAGAATTGACTGCACAACTGTGTTCAATTACAAGAGATGTTAGAAATGTACGTGGCAATACTCAGAAGAAGATTGAAAAGCTTAGACAGCTGCTTTCTGGTCTTCTTAGTGAACTTACATATTTTGAAGAGCCGATTAGATCACCACTGGCTCCAGGAGTCCTCATCAATGGGATTGTGCCATCGGAGTCATCAATATTCAAAAGTGCACTGCATCCTCTTCGCTTGACTTTCCGAGCAGCAAATGGGGGCAGTTGCAAAGTTATCTTCAAGAAGGGGGATGACATTCGCCAGGACCAATTAGTTGTTCAAATGGTATCTCTCATGGATCGATTGCTCAAAAACGAAAATCTTGATCTGCACTTAACTCCTTATAAGGTTCTTGCAACTGGGCAAGATGAAGGAATGATGGAATTCATTCCATCCCGCACGTTAGCGCTGATTCTCTCAGAGCATAGAAGTATTACGAGCTACCTGCAGAAGTTTCATCCAGATGAGCATGGACCATTTGGTATTACTGCCACCTGTCTGGAAACATTTATAAAAAGCTGTGCTGGCTACTCTGTAATCACATATATACTGGGCATTGGAGACAGGCATCTTGACAACCTTCTTCTTAGGGATGATGGAAGCCTTTTCCATGTTGATTTTGGCTTCATTCTTGGTCGTGATCCAAAGCCATTTCCACCACCAATGAAGCTTTGCAAAGAAATGGTTGAGGCTATGGGTGGAGCTGAAAGGTCGTTTATGATAGATTTTGACACTTTGATGCTTATCTATTATGTCATGTTTGCTGCACAGAATCCCATTAGATGTAGGAAATGGGATTCATATCATCTGGGGTGCAAAACTATGCAAATCTTTGATTTCTTTTTTTCCTTTTGCAGCCAGTACTATACAAGGTTCAAGTCCTACTGTTGTGAAGCATACAACATTATTCGGAAATCTAGTAACCTAATCTTGAATCTTTTTCATCTAATGGCGGGTTCCAACATTCCTGACATAGCGTCTGATCCTGAAAAAGGCATCCTCAAGGTAAGTTCCTTTATTATTGTAATCTTATTTATGTTGTTCAGTCTTGCAACTTTGGTCGTTTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000407 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005777 GO:0005942 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006914 GO:0006950 GO:0006970 GO:0006996 GO:0007033 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009056 GO:0009058 GO:0009555 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016236 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019637 GO:0019898 GO:0022607 GO:0023052 GO:0030242 GO:0030258 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0034271 GO:0034272 GO:0035004 GO:0035032 GO:0035556 GO:0036092 GO:0036211 GO:0042579 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045017 GO:0046474 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0052742 GO:0055046 GO:0061695 GO:0061919 GO:0065007 GO:0070925 GO:0071704 GO:0071840 GO:0072593 GO:0090407 GO:0097708 GO:0098657 GO:0098796 GO:1901564 GO:1901576 GO:1902494 GO:1905037 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 ko04136 ko04138 ko04140 ko04145 ko04371 ko05152 ko05167 map00562 map01100 map04070 map04136 map04138 map04140 map04145 map04371 map05152 map05167
Pfam Domains
Protein Families
PI3K_C2 PI3Ka PI3_PI4_kinase

Protein Analysis

842

Amino Acids

96.24

Weight (kDa)

7.2

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3K_C2 PF00792 50 - 192 1.7e-42 Phosphoinositide 3-kinase C2
PI3Ka PF00613 278 - 454 9.8e-67 Phosphoinositide 3-kinase family, accessory domain (PIK domain)
PI3_PI4_kinase PF00454 561 - 719 8.1e-46 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1800, 1967
Acc16I TGCGCA 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 1908
AccBSI CCGCTC 2 cut(s) 6, 70
AciI CCGC 6 cut(s) 6, 19, 70, 565, 1852, 2408
AclI AACGTT 1 cut(s) 502
AclWI GGATC 8 cut(s) 71, 113, 535, 840, 1295, 1761, 2090, 2432
AcoI YGGCCR 1 cut(s) 422
AcsI RAATTY 3 cut(s) 694, 932, 1838
AcuI CTGAAG 2 cut(s) 210, 1154
AfaI GTAC 7 cut(s) 176, 268, 635, 884, 1330, 1476, 2318
AfeI AGCGCT 2 cut(s) 1199, 1863
AfiI CCNNNNNNNGG 3 cut(s) 757, 758, 2447
AhdI GACNNNNNGTC 1 cut(s) 2431
AjnI CCWGG 7 cut(s) 424, 463, 636, 678, 1341, 1579, 1716
AjuI GAANNNNNNNTTGG 4 cut(s) 333, 365, 1716, 1748
AleI CACNNNNGTG 1 cut(s) 1444
Alw21I GWGCWC 3 cut(s) 85, 783, 1636
Alw26I GTCTC 2 cut(s) 1377, 2010
Alw44I GTGCAC 1 cut(s) 1632
AlwI GGATC 8 cut(s) 71, 113, 535, 840, 1295, 1761, 2090, 2432
AlwNI CAGNNNCTG 1 cut(s) 925
Aor51HI AGCGCT 2 cut(s) 1199, 1863
AoxI GGCC 4 cut(s) 92, 230, 422, 963
ApaI GGGCCC 1 cut(s) 967
ApaLI GTGCAC 1 cut(s) 1632
ApeKI GCWGC 5 cut(s) 919, 1516, 1664, 2219, 2310
ApoI RAATTY 3 cut(s) 694, 932, 1838
Asp700I GAANNNNTTC 3 cut(s) 14, 1104, 1842
AspLEI GCGC 4 cut(s) 170, 188, 1200, 1864
AspS9I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
AsuC2I CCSGG 1 cut(s) 478
AsuHPI GGTGA 3 cut(s) 46, 920, 1560
AsuNHI GCTAGC 1 cut(s) 1262
AvaII GGWCC 4 cut(s) 562, 748, 1720, 1928
BaeGI GKGCMC 2 cut(s) 967, 1636
BalI TGGCCA 1 cut(s) 424
BanII GRGCYC 2 cut(s) 85, 967
BarI GAAGNNNNNNTAC 2 cut(s) 1307, 1339
BbsI GAAGAC 1 cut(s) 1520
Bbv12I GWGCWC 3 cut(s) 85, 783, 1636
BbvI GCAGC 5 cut(s) 931, 1503, 1676, 2206, 2322
BcgI CGANNNNNNTGC 4 cut(s) 1628, 1662, 1882, 1916
BciT130I CCWGG 7 cut(s) 426, 465, 638, 680, 1343, 1581, 1718
BcnI CCSGG 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 1377, 2010
BfaI CTAG 3 cut(s) 515, 1263, 2373
BfmI CTRYAG 3 cut(s) 652, 714, 1901
BfoI RGCGCY 3 cut(s) 189, 1201, 1865
BfuAI ACCTGC 1 cut(s) 1908
BisI GCNGC 6 cut(s) 71, 920, 1517, 1665, 2220, 2311
BlsI GCNGC 6 cut(s) 72, 921, 1518, 1666, 2221, 2312
BmcAI AGTACT 2 cut(s) 268, 2318
Bme1390I CCNGG 8 cut(s) 426, 465, 478, 638, 680, 1343, 1581, 1718
Bme18I GGWCC 4 cut(s) 562, 748, 1720, 1928
BmeRI GACNNNNNGTC 1 cut(s) 2431
BmgT120I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
BmiI GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2413
BmrFI CCNGG 8 cut(s) 426, 465, 478, 638, 680, 1343, 1581, 1718
BmrI ACTGGG 2 cut(s) 1824, 2015
BmsI GCATC 6 cut(s) 673, 1054, 1648, 2032, 2184, 2460
BmtI GCTAGC 1 cut(s) 1266
BmuI ACTGGG 2 cut(s) 1824, 2015
BpiI GAAGAC 1 cut(s) 1520
BpmI CTGGAG 1 cut(s) 1563
BpuEI CTTGAG 3 cut(s) 377, 821, 2441
BpuMI CCSGG 1 cut(s) 478
Bsa29I ATCGAT 1 cut(s) 1756
BsaAI YACGTR 1 cut(s) 1478
BsaBI GATNNNNATC 4 cut(s) 117, 831, 2198, 2256
BsaJI CCNNGG 2 cut(s) 476, 814
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 3 cut(s) 757, 758, 2447
Bse118I RCCGGY 1 cut(s) 90
Bse1I ACTGG 5 cut(s) 292, 1578, 1819, 2010, 2314
Bse8I GATNNNNATC 4 cut(s) 117, 831, 2198, 2256
BseBI CCWGG 7 cut(s) 426, 465, 638, 680, 1343, 1581, 1718
BseCI ATCGAT 1 cut(s) 1756
BseDI CCNNGG 2 cut(s) 476, 814
BseGI GGATG 9 cut(s) 325, 707, 1396, 1639, 1711, 1847, 1912, 2053, 2451
BseJI GATNNNNATC 4 cut(s) 117, 831, 2198, 2256
BseLI CCNNNNNNNGG 3 cut(s) 757, 758, 2447
BseMII CTCAG 5 cut(s) 77, 254, 945, 1502, 1887
BseNI ACTGG 5 cut(s) 292, 1578, 1819, 2010, 2314
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 2 cut(s) 967, 1636
BseXI GCAGC 5 cut(s) 931, 1503, 1676, 2206, 2322
BseYI CCCAGC 1 cut(s) 757
BsgI GTGCAG 3 cut(s) 1422, 1769, 2205
BshFI GGCC 4 cut(s) 94, 232, 424, 965
BshVI ATCGAT 1 cut(s) 1756
BsiHKAI GWGCWC 3 cut(s) 85, 783, 1636
BsiSI CCGG 3 cut(s) 91, 128, 478
BslFI GGGAC 2 cut(s) 549, 761
BslI CCNNNNNNNGG 3 cut(s) 757, 758, 2447
BsmAI GTCTC 2 cut(s) 1377, 2010
BsmBI CGTCTC 1 cut(s) 1377
BsmFI GGGAC 2 cut(s) 549, 761
BsnI GGCC 4 cut(s) 94, 232, 424, 965
Bsp120I GGGCCC 1 cut(s) 963
Bsp1286I GDGCHC 4 cut(s) 85, 783, 967, 1636
Bsp1407I TGTACA 1 cut(s) 174
BspACI CCGC 6 cut(s) 6, 19, 70, 565, 1852, 2408
BspANI GGCC 4 cut(s) 94, 232, 424, 965
BspCNI CTCAG 5 cut(s) 78, 255, 946, 1501, 1886
BspDI ATCGAT 1 cut(s) 1756
BspLI GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2413
BspMAI CTGCAG 1 cut(s) 1905
BspMI ACCTGC 1 cut(s) 1908
BspOI GCTAGC 1 cut(s) 1266
BspPI GGATC 8 cut(s) 71, 113, 535, 840, 1295, 1761, 2090, 2432
BspQI GCTCTTC 3 cut(s) 139, 1191, 1548
BsrBI CCGCTC 2 cut(s) 6, 70
BsrFI RCCGGY 1 cut(s) 90
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 5 cut(s) 292, 1578, 1819, 2010, 2314
BssAI RCCGGY 1 cut(s) 90
BssECI CCNNGG 2 cut(s) 476, 814
Bst2UI CCWGG 7 cut(s) 426, 465, 638, 680, 1343, 1581, 1718
Bst4CI ACNGT 5 cut(s) 313, 742, 1328, 1446, 2342
Bst6I CTCTTC 7 cut(s) 139, 953, 1095, 1191, 1341, 1548, 1650
BstAUI TGTACA 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 1478
BstC8I GCNNGC 5 cut(s) 92, 200, 303, 1264, 1981
BstDEI CTNAG 9 cut(s) 86, 263, 270, 954, 1488, 1508, 1532, 1873, 2042
BstDSI CCRYGG 1 cut(s) 814
BstENI CCTNNNNNAGG 1 cut(s) 2445
BstF5I GGATG 9 cut(s) 325, 707, 1396, 1639, 1711, 1847, 1912, 2053, 2451
BstH2I RGCGCY 3 cut(s) 189, 1201, 1865
BstHHI GCGC 4 cut(s) 170, 188, 1200, 1864
BstMAI GTCTC 2 cut(s) 1377, 2010
BstMWI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
BstNI CCWGG 7 cut(s) 426, 465, 638, 680, 1343, 1581, 1718
BstSCI CCNGG 8 cut(s) 424, 463, 476, 636, 678, 1341, 1579, 1716
BstSFI CTRYAG 3 cut(s) 652, 714, 1901
BstSLI GKGCMC 2 cut(s) 967, 1636
BstV1I GCAGC 5 cut(s) 931, 1503, 1676, 2206, 2322
BstV2I GAAGAC 1 cut(s) 1520
BstX2I RGATCY 2 cut(s) 540, 832
BstXI CCANNNNNNTGG 1 cut(s) 1954
BstYI RGATCY 2 cut(s) 540, 832
Bsu15I ATCGAT 1 cut(s) 1756
BsuRI GGCC 4 cut(s) 94, 232, 424, 965
BsuTUI ATCGAT 1 cut(s) 1756
BtgI CCRYGG 1 cut(s) 814
BtgZI GCGATG 1 cut(s) 1077
BtsCI GGATG 9 cut(s) 325, 707, 1396, 1639, 1711, 1847, 1912, 2053, 2451
BtsI GCAGTG 1 cut(s) 1634
BtsIMutI CAGTG 4 cut(s) 738, 1129, 1571, 1634
BveI ACCTGC 1 cut(s) 1908
Cac8I GCNNGC 5 cut(s) 92, 200, 303, 1264, 1981
CaiI CAGNNNCTG 1 cut(s) 925
CfoI GCGC 4 cut(s) 170, 188, 1200, 1864
Cfr10I RCCGGY 1 cut(s) 90
Cfr13I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
ClaI ATCGAT 1 cut(s) 1756
CseI GACGC 2 cut(s) 1409, 2421
CsiI ACCWGGT 1 cut(s) 636
Csp6I GTAC 7 cut(s) 175, 267, 634, 883, 1329, 1475, 2317
CviAII CATG 5 cut(s) 1379, 1750, 1925, 2066, 2212
CviQI GTAC 7 cut(s) 175, 267, 634, 883, 1329, 1475, 2317
DdeI CTNAG 9 cut(s) 86, 263, 270, 954, 1488, 1508, 1532, 1873, 2042
DraI TTTAAA 2 cut(s) 577, 1399
DriI GACNNNNNGTC 1 cut(s) 2431
EaeI YGGCCR 1 cut(s) 422
Eam1104I CTCTTC 7 cut(s) 139, 953, 1095, 1191, 1341, 1548, 1650
Eam1105I GACNNNNNGTC 1 cut(s) 2431
EarI CTCTTC 7 cut(s) 139, 953, 1095, 1191, 1341, 1548, 1650
Ecl136II GAGCTC 1 cut(s) 83
Eco24I GRGCYC 2 cut(s) 85, 967
Eco47I GGWCC 4 cut(s) 562, 748, 1720, 1928
Eco47III AGCGCT 2 cut(s) 1199, 1863
Eco53kI GAGCTC 1 cut(s) 83
Eco57I CTGAAG 2 cut(s) 210, 1154
EcoICRI GAGCTC 1 cut(s) 83
EcoNI CCTNNNNNAGG 1 cut(s) 2445
EcoO109I RGGNCCY 4 cut(s) 230, 748, 963, 964
EcoRI GAATTC 1 cut(s) 1838
EcoRII CCWGG 7 cut(s) 424, 463, 636, 678, 1341, 1579, 1716
EcoT22I ATGCAT 1 cut(s) 1380
EcoT38I GRGCYC 2 cut(s) 85, 967
Esp3I CGTCTC 1 cut(s) 1377
FaeI CATG 5 cut(s) 1382, 1753, 1928, 2069, 2215
FaqI GGGAC 2 cut(s) 549, 761
FatI CATG 5 cut(s) 1378, 1749, 1924, 2065, 2211
FauI CCCGC 2 cut(s) 1859, 2401
FauNDI CATATG 2 cut(s) 1114, 1309
Fnu4HI GCNGC 6 cut(s) 71, 920, 1517, 1665, 2220, 2311
FokI GGATG 9 cut(s) 332, 694, 1403, 1626, 1718, 1834, 1899, 2060, 2438
FriOI GRGCYC 2 cut(s) 85, 967
Fsp4HI GCNGC 6 cut(s) 71, 920, 1517, 1665, 2220, 2311
FspAI RTGCGCAY 1 cut(s) 169
FspBI CTAG 3 cut(s) 515, 1263, 2373
FspI TGCGCA 1 cut(s) 169
GlaI GCGC 4 cut(s) 169, 187, 1199, 1863
GluI GCNGC 6 cut(s) 71, 920, 1517, 1665, 2220, 2311
GsaI CCCAGC 1 cut(s) 761
GsuI CTGGAG 1 cut(s) 1563
HaeII RGCGCY 3 cut(s) 189, 1201, 1865
HaeIII GGCC 4 cut(s) 94, 232, 424, 965
HapII CCGG 3 cut(s) 91, 128, 478
HgaI GACGC 2 cut(s) 1409, 2421
HhaI GCGC 4 cut(s) 170, 188, 1200, 1864
Hin1II CATG 5 cut(s) 1382, 1753, 1928, 2069, 2215
Hin6I GCGC 4 cut(s) 168, 186, 1198, 1862
HinP1I GCGC 4 cut(s) 168, 186, 1198, 1862
HindIII AAGCTT 2 cut(s) 1505, 2123
HpaII CCGG 3 cut(s) 91, 128, 478
HphI GGTGA 3 cut(s) 46, 920, 1560
Hpy166II GTNNAC 3 cut(s) 102, 1538, 1634
Hpy8I GTNNAC 3 cut(s) 102, 1538, 1634
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 6 cut(s) 71, 243, 335, 1693, 1820, 2045
HpyCH4III ACNGT 5 cut(s) 313, 742, 1328, 1446, 2342
HpyCH4IV ACGT 6 cut(s) 487, 502, 601, 1237, 1477, 1856
HpyF10VI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
HpyF3I CTNAG 9 cut(s) 86, 263, 270, 954, 1488, 1508, 1532, 1873, 2042
HpySE526I ACGT 6 cut(s) 487, 502, 601, 1237, 1477, 1856
Hsp92II CATG 5 cut(s) 1382, 1753, 1928, 2069, 2215
HspAI GCGC 4 cut(s) 168, 186, 1198, 1862
KflI GGGWCCC 1 cut(s) 748
KroI GCCGGC 1 cut(s) 90
KroNI GCCGGC 1 cut(s) 92
LguI GCTCTTC 3 cut(s) 139, 1191, 1548
LmnI GCTCC 6 cut(s) 80, 193, 851, 1072, 1582, 2156
Lsp1109I GCAGC 5 cut(s) 931, 1503, 1676, 2206, 2322
LweI GCATC 6 cut(s) 673, 1054, 1648, 2032, 2184, 2460
MabI ACCWGGT 1 cut(s) 636
MaeI CTAG 3 cut(s) 515, 1263, 2373
MaeII ACGT 6 cut(s) 487, 502, 601, 1237, 1477, 1856
MaeIII GTNAC 5 cut(s) 290, 736, 908, 1160, 2375
MbiI CCGCTC 2 cut(s) 6, 70
MflI RGATCY 2 cut(s) 540, 832
MhlI GDGCHC 4 cut(s) 85, 783, 967, 1636
MlsI TGGCCA 1 cut(s) 424
MluCI AATT 9 cut(s) 694, 732, 932, 1028, 1400, 1430, 1452, 1724, 1838
MluNI TGGCCA 1 cut(s) 424
MlyI GAGTC 3 cut(s) 454, 1593, 1622
MmeI TCCRAC 6 cut(s) 146, 224, 477, 914, 1050, 2439
Mox20I TGGCCA 1 cut(s) 424
Mph1103I ATGCAT 1 cut(s) 1380
MroNI GCCGGC 1 cut(s) 90
MroXI GAANNNNTTC 3 cut(s) 14, 1104, 1842
MscI TGGCCA 1 cut(s) 424
MseI TTAA 5 cut(s) 375, 576, 809, 1398, 1790
MslI CAYNNNNRTG 3 cut(s) 1444, 1917, 2192
Msp20I TGGCCA 1 cut(s) 424
MspA1I CMGCKG 3 cut(s) 1174, 1370, 1516
MspI CCGG 3 cut(s) 91, 128, 478
MspR9I CCNGG 8 cut(s) 426, 465, 478, 638, 680, 1343, 1581, 1718
MvaI CCWGG 7 cut(s) 426, 465, 638, 680, 1343, 1581, 1718
MwoI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
NaeI GCCGGC 1 cut(s) 92
NciI CCSGG 1 cut(s) 478
NdeI CATATG 2 cut(s) 1114, 1309
NgoMIV GCCGGC 1 cut(s) 90
NheI GCTAGC 1 cut(s) 1262
NlaIII CATG 5 cut(s) 1382, 1753, 1928, 2069, 2215
NlaIV GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2413
NmuCI GTSAC 3 cut(s) 290, 736, 908
NsbI TGCGCA 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 1380
OliI CACNNNNGTG 1 cut(s) 1444
PciSI GCTCTTC 3 cut(s) 139, 1191, 1548
PcsI WCGNNNNNNNCGW 1 cut(s) 1243
PdiI GCCGGC 1 cut(s) 92
PdmI GAANNNNTTC 3 cut(s) 14, 1104, 1842
PfeI GAWTC 9 cut(s) 221, 364, 683, 874, 1191, 1867, 2227, 2252, 2389
PfoI TCCNGGA 4 cut(s) 463, 678, 1341, 1579
PkrI GCNGC 6 cut(s) 72, 921, 1518, 1666, 2221, 2312
PleI GAGTC 3 cut(s) 454, 1592, 1621
PpsI GAGTC 3 cut(s) 454, 1592, 1621
Ppu21I YACGTR 1 cut(s) 1478
PpuMI RGGWCCY 1 cut(s) 748
PsiI TTATAA 2 cut(s) 1800, 1967
Psp124BI GAGCTC 1 cut(s) 85
Psp1406I AACGTT 1 cut(s) 502
Psp5II RGGWCCY 1 cut(s) 748
Psp6I CCWGG 7 cut(s) 424, 463, 636, 678, 1341, 1579, 1716
PspFI CCCAGC 1 cut(s) 757
PspGI CCWGG 7 cut(s) 424, 463, 636, 678, 1341, 1579, 1716
PspN4I GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2413
PspOMI GGGCCC 1 cut(s) 963
PspPI GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
PspPPI RGGWCCY 1 cut(s) 748
PstI CTGCAG 1 cut(s) 1905
PstNI CAGNNNCTG 1 cut(s) 925
PsuI RGATCY 2 cut(s) 540, 832
PvuII CAGCTG 3 cut(s) 1174, 1370, 1516
RsaI GTAC 7 cut(s) 176, 268, 635, 884, 1330, 1476, 2318
RsaNI GTAC 7 cut(s) 175, 267, 634, 883, 1329, 1475, 2317
RseI CAYNNNNRTG 3 cut(s) 1444, 1917, 2192
SacI GAGCTC 1 cut(s) 85
SapI GCTCTTC 3 cut(s) 139, 1191, 1548
SaqAI TTAA 5 cut(s) 375, 576, 809, 1398, 1790
SatI GCNGC 6 cut(s) 71, 920, 1517, 1665, 2220, 2311
Sau96I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
ScaI AGTACT 2 cut(s) 268, 2318
SchI GAGTC 3 cut(s) 454, 1593, 1622
ScrFI CCNGG 8 cut(s) 426, 465, 478, 638, 680, 1343, 1581, 1718
SduI GDGCHC 4 cut(s) 85, 783, 967, 1636
SexAI ACCWGGT 1 cut(s) 636
SfaNI GCATC 6 cut(s) 673, 1054, 1648, 2032, 2184, 2460
SfcI CTRYAG 3 cut(s) 652, 714, 1901
SinI GGWCC 4 cut(s) 562, 748, 1720, 1928
SmiI ATTTAAAT 1 cut(s) 1399
SmiMI CAYNNNNRTG 3 cut(s) 1444, 1917, 2192
SmlI CTYRAG 3 cut(s) 392, 836, 2456
SmoI CTYRAG 3 cut(s) 392, 836, 2456
Sse9I AATT 9 cut(s) 694, 732, 932, 1028, 1400, 1430, 1452, 1724, 1838
SsiI CCGC 6 cut(s) 6, 19, 70, 565, 1852, 2408
SspI AATATT 1 cut(s) 1623
SspMI CTAG 3 cut(s) 515, 1263, 2373
SstI GAGCTC 1 cut(s) 85
StyD4I CCNGG 8 cut(s) 424, 463, 476, 636, 678, 1341, 1579, 1716
SwaI ATTTAAAT 1 cut(s) 1399
TaaI ACNGT 5 cut(s) 313, 742, 1328, 1446, 2342
TaiI ACGT 6 cut(s) 490, 505, 604, 1240, 1480, 1859
TaqI TCGA 7 cut(s) 66, 458, 645, 863, 1189, 1194, 1756
TasI AATT 9 cut(s) 694, 732, 932, 1028, 1400, 1430, 1452, 1724, 1838
TatI WGTACW 3 cut(s) 174, 266, 2316
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 9 cut(s) 221, 364, 683, 874, 1191, 1867, 2227, 2252, 2389
Tru1I TTAA 5 cut(s) 375, 576, 809, 1398, 1790
Tru9I TTAA 5 cut(s) 375, 576, 809, 1398, 1790
TscAI CASTG 4 cut(s) 745, 1129, 1578, 1641
TseFI GTSAC 3 cut(s) 290, 736, 908
TseI GCWGC 5 cut(s) 919, 1516, 1664, 2219, 2310
Tsp45I GTSAC 3 cut(s) 290, 736, 908
TspGWI ACGGA 3 cut(s) 149, 180, 223
TspRI CASTG 4 cut(s) 745, 1129, 1578, 1641
VneI GTGCAC 1 cut(s) 1632
VpaK11BI GGWCC 4 cut(s) 562, 748, 1720, 1928
XagI CCTNNNNNAGG 1 cut(s) 2445
XapI RAATTY 3 cut(s) 694, 932, 1838
XcmI CCANNNNNNNNNTGG 1 cut(s) 2072
XmnI GAANNNNTTC 3 cut(s) 14, 1104, 1842
XspI CTAG 3 cut(s) 515, 1263, 2373
ZrmI AGTACT 2 cut(s) 268, 2318
Zsp2I ATGCAT 1 cut(s) 1380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.