Rroxscaffold_5G00384200

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010181
Physical Location & Seq
Forward (+)
63697144 .. 63704913
7770 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384200.1

Sequence Viewer

Length: 2451 bp
ATGAGCGGGAACGAGTTCCGCTTCTTCTTGTCCTGCGACATCAATCTCCCACTCACCTTTCGGATCGAGCGGCTTGAAGGAGCTCTGACCCGGCCCAAGTCCACAACCTCCGATGTTGATCCAACTCCGGCGACGGAGGAGAGAAGAGCAGAGCTGTATGTGGAGTGCGCATTGTACATTGATGGCGCTCCGTTTGGCTTGCCCACACGGACAAGGCTGGAATCTTCAGGACCTTCATATTGTTGGAACGAGCTGATTACCCTGAGTACTAAGTATAATGACTTGACTGGTCACTCACAGCTTGCTTTTACTGTTTGGGATGTTTCGTGTGGGAAAGATGAAGGGTTGATTGGTGGTGCTACGATTCTTCTCTTTAATAACAAGAAGCAACTCAAAACAGGGAAGCAAAAGCTGAGGCTTTGGAAAGGAAAAGTAGCTGATGGGTCATTTCCTACTTCGACTCCCGGAAAGGTTCCCCGGAATGAACGTGGGGAGTTGGAGCGTTTGGAGAAGCTAGTGAATAAGTATGAGAGGGGACAGATCCAATGTGTTGATTGGCTGGACCGCCTTGCATTTAAAGCTATGGAGAGAATAAAGGAACGTGAAAGCTCTAAAAATGGAAGTTTGCATTTGTACCTGGTTGTCGATTTCTGTAGTTTTGAGCATCGTGTTGTTTTCCAGGAATCGGGGGCAAATTTCTTATTACCATCCCCTATAGCTTCCACGAATGAAATTGTCACTGTTTGGGACCCAGAGTTGGGAAAGATAAATCCATCAGAGCACAAGCAACTAAAACTTGCAAGGAGTTTAACCCGTGGTATCATTGATAGGGATCTCAAGCCAAGCTCCAATGAGAGAAAGTCAATACAGAGGATTCTAAAGTACCCTCCAACAAGGACATTGAGTGGTGACGAAAGGCAGCTTCTGTGGAAATTTCGTTTCTCATTGATGTCTGAGAAGAGGGCCCTCACAAAGTTCCTCAAATGTGTTGAATGGAGTGATGTTCAGGAAGCAAAACAAGCATTAGAATTGATGGGCAGATGGGAAATGATTGATGTTTGCGATGCGTTGGAGCTGTTATCTCCTGTTTTTGAAAGTGAAGAGGTTCGTGCATATGCTGTCAGTGTTCTTGAAAGAGCTGATGATGAAGAACTCCAATGTTACTTACTTCAGCTGGTTCAGGCTCTTCGATTCGAGCGCTCTGATAAATCCTGCCTTTCTCATTTCCTTGTTCAACGTGCATTACGAAACATTGAGTTGGCTAGCTTTCTTCGCTGGTATGTTGCTGTGGAACTTCATGATCCAGCATATGCGAAGCGTTTTTACTGTACCTACGAACTCCTGGAAGAGAATATGATGAAGTTGTCAGCTGGTATGCATGGAGACGAGGATGGATTTAAATTGTGGCAAAGTTTGGTGCGTCAGACAGAATTGACTGCACAACTGTGTTCAATTACAAGAGATGTTAGAAATGTACGTGGCAATACTCAGAAGAAGATTGAAAAGCTTAGACAGCTGCTTTCTGGTCTTCTTAGTGAACTTACATATTTTGAAGAGCCGATTCGATCGCCACTGGCTCCAGGAGTCCTCATCAATGGGATTGTGCCATCGGAGTCATCAATATTCAAAAGTGCACTGCATCCTCTTCGCTTGACTTTCCGAGCAGCAAATGGGGGCAGTTGCAAAGTTATCTTCAAGAAGGGGGATGACATTCGCCAGGACCAATTAGTTGTTCAAATGGTATCTCTCATGGATCGATTGCTCAAAAATGAAAATCTTGATCTGCACTTAACTCCTTATAAGGTTCTTGCAACTGGGCAAGATGAAGGAATGATGGAATTCATTCCATCCCGCACGTTAGCGCTGATTCTCTCAGAGCATAGAAGTATTACGAGCTACCTGCAGAAGTTTCATCCAGATGAACATGGACCATTTGGTATTACTGCCACCTGTCTGGAAACATTTATAAAAAGCTGTGCTGGCTACTCTGTTATCACATATATACTGGGCATTGGAGACAGGCATCTTGACAACCTTCTTCTTAGGGATGATGGAAGCCTTTTCCATGTTGATTTTGGCTTCATTCTTGGTCGTGATCCAAAGCCATTTCCACCACCAATGAAGCTTTGCAAAGAAATGGTTGAGGCTATGGGTGGAGCTGAAAGCCAGTACTATACAAGGTTCAAGTCTTACTGTTGTGAAGCATACAACATTATTCGGAAATCTAGTAACCTAATCTTGAATCTTTTTCATCTAATGGCGGGTTCCAACATTCCTGACATAGCGTCTGATCCTGAAAAAGGCATCCTCAAGCTCCAGGAGAAATTCCGGTTGGACTTAGACGATGAAGCCTGCATACACTTCTTCCAGGACTTGATCAATGAGAGTGTTAGTGCACTGTTTCCTCAAATGGTTGAGACCATTCATCGGTGGGCTCAATATTGGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000407 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005777 GO:0005942 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006914 GO:0006950 GO:0006970 GO:0006996 GO:0007033 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009056 GO:0009058 GO:0009555 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016236 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019637 GO:0019898 GO:0022607 GO:0023052 GO:0030242 GO:0030258 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0034271 GO:0034272 GO:0035004 GO:0035032 GO:0035556 GO:0036092 GO:0036211 GO:0042579 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045017 GO:0046474 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0052742 GO:0055046 GO:0061695 GO:0061919 GO:0065007 GO:0070925 GO:0071704 GO:0071840 GO:0072593 GO:0090407 GO:0097708 GO:0098657 GO:0098796 GO:1901564 GO:1901576 GO:1902494 GO:1905037 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 ko04136 ko04138 ko04140 ko04145 ko04371 ko05152 ko05167 map00562 map01100 map04070 map04136 map04138 map04140 map04145 map04371 map05152 map05167
Pfam Domains
Protein Families
PI3K_C2 PI3Ka PI3_PI4_kinase

Protein Analysis

816

Amino Acids

93.32

Weight (kDa)

6.54

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3K_C2 PF00792 50 - 192 3.2e-42 Phosphoinositide 3-kinase C2
PI3Ka PF00613 278 - 454 9.3e-67 Phosphoinositide 3-kinase family, accessory domain (PIK domain)
PI3_PI4_kinase PF00454 561 - 762 2.1e-58 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1800, 1967
Acc16I TGCGCA 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 1908
AccBSI CCGCTC 2 cut(s) 6, 70
AciI CCGC 6 cut(s) 6, 19, 70, 565, 1852, 2261
AclWI GGATC 8 cut(s) 71, 113, 535, 840, 1295, 1761, 2090, 2285
AcsI RAATTY 4 cut(s) 694, 932, 1838, 2324
AcuI CTGAAG 2 cut(s) 210, 1154
AfaI GTAC 7 cut(s) 176, 268, 635, 884, 1330, 1476, 2171
AfeI AGCGCT 2 cut(s) 1199, 1863
AfiI CCNNNNNNNGG 5 cut(s) 685, 757, 758, 2300, 2427
AhdI GACNNNNNGTC 1 cut(s) 2284
AjnI CCWGG 7 cut(s) 636, 678, 1341, 1579, 1716, 2316, 2367
AjuI GAANNNNNNNTTGG 6 cut(s) 333, 365, 1716, 1748, 2315, 2347
AleI CACNNNNGTG 1 cut(s) 1444
Alw21I GWGCWC 4 cut(s) 85, 783, 1636, 2398
Alw26I GTCTC 3 cut(s) 1377, 2010, 2411
Alw44I GTGCAC 2 cut(s) 1632, 2394
AlwI GGATC 8 cut(s) 71, 113, 535, 840, 1295, 1761, 2090, 2285
AlwNI CAGNNNCTG 1 cut(s) 925
Aor51HI AGCGCT 2 cut(s) 1199, 1863
AoxI GGCC 2 cut(s) 92, 963
ApaI GGGCCC 1 cut(s) 967
ApaLI GTGCAC 2 cut(s) 1632, 2394
ApeKI GCWGC 3 cut(s) 919, 1516, 1664
ApoI RAATTY 4 cut(s) 694, 932, 1838, 2324
Asp700I GAANNNNTTC 3 cut(s) 14, 1104, 1842
AspLEI GCGC 5 cut(s) 170, 188, 1200, 1864, 2448
AspS9I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
AsuC2I CCSGG 3 cut(s) 91, 465, 478
AsuHPI GGTGA 2 cut(s) 46, 920
AsuNHI GCTAGC 1 cut(s) 1262
AvaII GGWCC 5 cut(s) 230, 562, 748, 1720, 1928
BaeGI GKGCMC 3 cut(s) 967, 1636, 2398
BanII GRGCYC 3 cut(s) 85, 967, 2437
BarI GAAGNNNNNNTAC 4 cut(s) 1307, 1339, 2340, 2372
BbsI GAAGAC 1 cut(s) 1520
Bbv12I GWGCWC 4 cut(s) 85, 783, 1636, 2398
BbvCI CCTCAGC 1 cut(s) 413
BbvI GCAGC 3 cut(s) 931, 1503, 1676
BcgI CGANNNNNNTGC 4 cut(s) 1628, 1662, 1882, 1916
BciT130I CCWGG 7 cut(s) 638, 680, 1343, 1581, 1718, 2318, 2369
BclI TGATCA 1 cut(s) 2376
BcnI CCSGG 3 cut(s) 91, 465, 478
BcoDI GTCTC 3 cut(s) 1377, 2010, 2411
BfaI CTAG 3 cut(s) 515, 1263, 2226
BfmI CTRYAG 3 cut(s) 652, 714, 1901
BfoI RGCGCY 4 cut(s) 189, 1201, 1865, 2449
BfuAI ACCTGC 1 cut(s) 1908
BisI GCNGC 4 cut(s) 71, 920, 1517, 1665
BlsI GCNGC 4 cut(s) 72, 921, 1518, 1666
BmcAI AGTACT 2 cut(s) 268, 2171
Bme18I GGWCC 5 cut(s) 230, 562, 748, 1720, 1928
BmeRI GACNNNNNGTC 1 cut(s) 2284
BmgT120I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
BmiI GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2266
BmrI ACTGGG 2 cut(s) 1824, 2015
BmsI GCATC 5 cut(s) 673, 1054, 1648, 2032, 2313
BmtI GCTAGC 1 cut(s) 1266
BmuI ACTGGG 2 cut(s) 1824, 2015
BpiI GAAGAC 1 cut(s) 1520
BpmI CTGGAG 2 cut(s) 1563, 2300
Bpu10I CCTNAGC 1 cut(s) 413
BpuEI CTTGAG 2 cut(s) 821, 2294
BpuMI CCSGG 3 cut(s) 91, 465, 478
Bsa29I ATCGAT 1 cut(s) 1756
BsaAI YACGTR 1 cut(s) 1478
BsaBI GATNNNNATC 2 cut(s) 117, 831
BsaI GGTCTC 1 cut(s) 2411
BsaJI CCNNGG 2 cut(s) 476, 814
BsaWI WCCGGW 1 cut(s) 2328
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 5 cut(s) 685, 757, 758, 2300, 2427
Bse1I ACTGG 5 cut(s) 292, 1578, 1819, 2010, 2167
Bse8I GATNNNNATC 2 cut(s) 117, 831
BseBI CCWGG 7 cut(s) 638, 680, 1343, 1581, 1718, 2318, 2369
BseCI ATCGAT 1 cut(s) 1756
BseDI CCNNGG 2 cut(s) 476, 814
BseGI GGATG 9 cut(s) 325, 707, 1396, 1639, 1711, 1847, 1912, 2053, 2304
BseJI GATNNNNATC 2 cut(s) 117, 831
BseLI CCNNNNNNNGG 5 cut(s) 685, 757, 758, 2300, 2427
BseMII CTCAG 5 cut(s) 254, 404, 945, 1502, 1887
BseNI ACTGG 5 cut(s) 292, 1578, 1819, 2010, 2167
BseRI GAGGAG 1 cut(s) 152
BseSI GKGCMC 3 cut(s) 967, 1636, 2398
BseXI GCAGC 3 cut(s) 931, 1503, 1676
BsgI GTGCAG 2 cut(s) 1422, 1769
Bsh1285I CGRYCG 1 cut(s) 1568
BshFI GGCC 2 cut(s) 94, 965
BshVI ATCGAT 1 cut(s) 1756
BsiEI CGRYCG 1 cut(s) 1568
BsiHKAI GWGCWC 4 cut(s) 85, 783, 1636, 2398
BsiSI CCGG 5 cut(s) 91, 128, 465, 478, 2329
BslFI GGGAC 2 cut(s) 549, 761
BslI CCNNNNNNNGG 5 cut(s) 685, 757, 758, 2300, 2427
BsmAI GTCTC 3 cut(s) 1377, 2010, 2411
BsmBI CGTCTC 1 cut(s) 1377
BsmFI GGGAC 2 cut(s) 549, 761
BsnI GGCC 2 cut(s) 94, 965
Bso31I GGTCTC 1 cut(s) 2411
Bsp120I GGGCCC 1 cut(s) 963
Bsp1286I GDGCHC 6 cut(s) 85, 783, 967, 1636, 2398, 2437
Bsp1407I TGTACA 1 cut(s) 174
BspACI CCGC 6 cut(s) 6, 19, 70, 565, 1852, 2261
BspANI GGCC 2 cut(s) 94, 965
BspCNI CTCAG 5 cut(s) 255, 405, 946, 1501, 1886
BspDI ATCGAT 1 cut(s) 1756
BspHI TCATGA 1 cut(s) 1297
BspLI GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2266
BspMAI CTGCAG 1 cut(s) 1905
BspMI ACCTGC 1 cut(s) 1908
BspOI GCTAGC 1 cut(s) 1266
BspPI GGATC 8 cut(s) 71, 113, 535, 840, 1295, 1761, 2090, 2285
BspQI GCTCTTC 3 cut(s) 139, 1191, 1548
BspTNI GGTCTC 1 cut(s) 2411
BsrBI CCGCTC 2 cut(s) 6, 70
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 5 cut(s) 292, 1578, 1819, 2010, 2167
BssECI CCNNGG 2 cut(s) 476, 814
Bst2UI CCWGG 7 cut(s) 638, 680, 1343, 1581, 1718, 2318, 2369
Bst4CI ACNGT 6 cut(s) 313, 742, 1328, 1446, 2195, 2400
Bst6I CTCTTC 7 cut(s) 139, 953, 1095, 1191, 1341, 1548, 1650
BstAUI TGTACA 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 1478
BstC8I GCNNGC 5 cut(s) 200, 303, 1264, 1981, 2353
BstDSI CCRYGG 1 cut(s) 814
BstENI CCTNNNNNAGG 1 cut(s) 2298
BstF5I GGATG 9 cut(s) 325, 707, 1396, 1639, 1711, 1847, 1912, 2053, 2304
BstH2I RGCGCY 4 cut(s) 189, 1201, 1865, 2449
BstHHI GCGC 5 cut(s) 170, 188, 1200, 1864, 2448
BstMAI GTCTC 3 cut(s) 1377, 2010, 2411
BstMCI CGRYCG 1 cut(s) 1568
BstMWI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
BstNI CCWGG 7 cut(s) 638, 680, 1343, 1581, 1718, 2318, 2369
BstSFI CTRYAG 3 cut(s) 652, 714, 1901
BstSLI GKGCMC 3 cut(s) 967, 1636, 2398
BstV1I GCAGC 3 cut(s) 931, 1503, 1676
BstV2I GAAGAC 1 cut(s) 1520
BstX2I RGATCY 2 cut(s) 540, 832
BstXI CCANNNNNNTGG 1 cut(s) 1954
BstYI RGATCY 2 cut(s) 540, 832
Bsu15I ATCGAT 1 cut(s) 1756
BsuRI GGCC 2 cut(s) 94, 965
BsuTUI ATCGAT 1 cut(s) 1756
BtgI CCRYGG 1 cut(s) 814
BtgZI GCGATG 1 cut(s) 1077
BtsCI GGATG 9 cut(s) 325, 707, 1396, 1639, 1711, 1847, 1912, 2053, 2304
BtsI GCAGTG 1 cut(s) 1634
BtsIMutI CAGTG 5 cut(s) 738, 1129, 1571, 1634, 2396
BveI ACCTGC 1 cut(s) 1908
Cac8I GCNNGC 5 cut(s) 200, 303, 1264, 1981, 2353
CaiI CAGNNNCTG 1 cut(s) 925
CciI TCATGA 1 cut(s) 1297
CfoI GCGC 5 cut(s) 170, 188, 1200, 1864, 2448
Cfr13I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
ClaI ATCGAT 1 cut(s) 1756
CseI GACGC 2 cut(s) 1409, 2274
CsiI ACCWGGT 1 cut(s) 636
Csp6I GTAC 7 cut(s) 175, 267, 634, 883, 1329, 1475, 2170
CviAII CATG 5 cut(s) 1298, 1379, 1750, 1925, 2066
CviQI GTAC 7 cut(s) 175, 267, 634, 883, 1329, 1475, 2170
DraI TTTAAA 2 cut(s) 577, 1399
DriI GACNNNNNGTC 1 cut(s) 2284
Eam1104I CTCTTC 7 cut(s) 139, 953, 1095, 1191, 1341, 1548, 1650
Eam1105I GACNNNNNGTC 1 cut(s) 2284
EarI CTCTTC 7 cut(s) 139, 953, 1095, 1191, 1341, 1548, 1650
Ecl136II GAGCTC 1 cut(s) 83
Eco24I GRGCYC 3 cut(s) 85, 967, 2437
Eco31I GGTCTC 1 cut(s) 2411
Eco47I GGWCC 5 cut(s) 230, 562, 748, 1720, 1928
Eco47III AGCGCT 2 cut(s) 1199, 1863
Eco53kI GAGCTC 1 cut(s) 83
Eco57I CTGAAG 2 cut(s) 210, 1154
EcoICRI GAGCTC 1 cut(s) 83
EcoNI CCTNNNNNAGG 1 cut(s) 2298
EcoO109I RGGNCCY 4 cut(s) 230, 748, 963, 964
EcoRI GAATTC 1 cut(s) 1838
EcoRII CCWGG 7 cut(s) 636, 678, 1341, 1579, 1716, 2316, 2367
EcoT22I ATGCAT 1 cut(s) 1380
EcoT38I GRGCYC 3 cut(s) 85, 967, 2437
Esp3I CGTCTC 1 cut(s) 1377
FaeI CATG 5 cut(s) 1301, 1382, 1753, 1928, 2069
FaqI GGGAC 2 cut(s) 549, 761
FatI CATG 5 cut(s) 1297, 1378, 1749, 1924, 2065
FauI CCCGC 2 cut(s) 1859, 2254
FauNDI CATATG 2 cut(s) 1114, 1309
FbaI TGATCA 1 cut(s) 2376
Fnu4HI GCNGC 4 cut(s) 71, 920, 1517, 1665
FokI GGATG 9 cut(s) 332, 694, 1403, 1626, 1718, 1834, 1899, 2060, 2291
FriOI GRGCYC 3 cut(s) 85, 967, 2437
Fsp4HI GCNGC 4 cut(s) 71, 920, 1517, 1665
FspAI RTGCGCAY 1 cut(s) 169
FspBI CTAG 3 cut(s) 515, 1263, 2226
FspI TGCGCA 1 cut(s) 169
GlaI GCGC 5 cut(s) 169, 187, 1199, 1863, 2447
GluI GCNGC 4 cut(s) 71, 920, 1517, 1665
GsuI CTGGAG 2 cut(s) 1563, 2300
HaeII RGCGCY 4 cut(s) 189, 1201, 1865, 2449
HaeIII GGCC 2 cut(s) 94, 965
HapII CCGG 5 cut(s) 91, 128, 465, 478, 2329
HgaI GACGC 2 cut(s) 1409, 2274
HhaI GCGC 5 cut(s) 170, 188, 1200, 1864, 2448
Hin1II CATG 5 cut(s) 1301, 1382, 1753, 1928, 2069
Hin6I GCGC 5 cut(s) 168, 186, 1198, 1862, 2446
HinP1I GCGC 5 cut(s) 168, 186, 1198, 1862, 2446
HindIII AAGCTT 2 cut(s) 1505, 2123
HpaII CCGG 5 cut(s) 91, 128, 465, 478, 2329
HphI GGTGA 2 cut(s) 46, 920
Hpy166II GTNNAC 4 cut(s) 102, 1538, 1634, 2396
Hpy8I GTNNAC 4 cut(s) 102, 1538, 1634, 2396
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 6 cut(s) 71, 243, 335, 1693, 1820, 2045
HpyCH4III ACNGT 6 cut(s) 313, 742, 1328, 1446, 2195, 2400
HpyCH4IV ACGT 5 cut(s) 487, 601, 1237, 1477, 1856
HpyF10VI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
HpySE526I ACGT 5 cut(s) 487, 601, 1237, 1477, 1856
Hsp92II CATG 5 cut(s) 1301, 1382, 1753, 1928, 2069
HspAI GCGC 5 cut(s) 168, 186, 1198, 1862, 2446
KflI GGGWCCC 1 cut(s) 748
Ksp22I TGATCA 1 cut(s) 2376
LguI GCTCTTC 3 cut(s) 139, 1191, 1548
LmnI GCTCC 8 cut(s) 80, 193, 499, 851, 1072, 1582, 2156, 2319
Lsp1109I GCAGC 3 cut(s) 931, 1503, 1676
LweI GCATC 5 cut(s) 673, 1054, 1648, 2032, 2313
MabI ACCWGGT 1 cut(s) 636
MaeI CTAG 3 cut(s) 515, 1263, 2226
MaeII ACGT 5 cut(s) 487, 601, 1237, 1477, 1856
MaeIII GTNAC 5 cut(s) 290, 736, 908, 1160, 2228
MbiI CCGCTC 2 cut(s) 6, 70
MflI RGATCY 2 cut(s) 540, 832
MhlI GDGCHC 6 cut(s) 85, 783, 967, 1636, 2398, 2437
MlyI GAGTC 3 cut(s) 454, 1593, 1622
MmeI TCCRAC 7 cut(s) 146, 224, 477, 914, 1050, 2292, 2313
Mph1103I ATGCAT 1 cut(s) 1380
MroXI GAANNNNTTC 3 cut(s) 14, 1104, 1842
MseI TTAA 5 cut(s) 375, 576, 809, 1398, 1790
MslI CAYNNNNRTG 2 cut(s) 1444, 1917
MspA1I CMGCKG 3 cut(s) 1174, 1370, 1516
MspI CCGG 5 cut(s) 91, 128, 465, 478, 2329
MvaI CCWGG 7 cut(s) 638, 680, 1343, 1581, 1718, 2318, 2369
MwoI GCNNNNNNNGC 5 cut(s) 578, 1019, 1272, 1513, 1980
NciI CCSGG 3 cut(s) 91, 465, 478
NdeI CATATG 2 cut(s) 1114, 1309
NheI GCTAGC 1 cut(s) 1262
NlaIII CATG 5 cut(s) 1301, 1382, 1753, 1928, 2069
NlaIV GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2266
NmuCI GTSAC 3 cut(s) 290, 736, 908
NsbI TGCGCA 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 1380
OliI CACNNNNGTG 1 cut(s) 1444
PagI TCATGA 1 cut(s) 1297
PciSI GCTCTTC 3 cut(s) 139, 1191, 1548
PcsI WCGNNNNNNNCGW 1 cut(s) 1243
PdmI GAANNNNTTC 3 cut(s) 14, 1104, 1842
PfeI GAWTC 8 cut(s) 221, 364, 683, 874, 1191, 1561, 1867, 2242
PfoI TCCNGGA 6 cut(s) 463, 678, 1341, 1579, 2316, 2367
PkrI GCNGC 4 cut(s) 72, 921, 1518, 1666
Ple19I CGATCG 1 cut(s) 1568
PleI GAGTC 3 cut(s) 454, 1592, 1621
PpsI GAGTC 3 cut(s) 454, 1592, 1621
Ppu21I YACGTR 1 cut(s) 1478
PpuMI RGGWCCY 2 cut(s) 230, 748
PsiI TTATAA 2 cut(s) 1800, 1967
Psp124BI GAGCTC 1 cut(s) 85
Psp5II RGGWCCY 2 cut(s) 230, 748
Psp6I CCWGG 7 cut(s) 636, 678, 1341, 1579, 1716, 2316, 2367
PspGI CCWGG 7 cut(s) 636, 678, 1341, 1579, 1716, 2316, 2367
PspN4I GGNNCC 6 cut(s) 474, 749, 750, 965, 1578, 2266
PspOMI GGGCCC 1 cut(s) 963
PspPI GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
PspPPI RGGWCCY 2 cut(s) 230, 748
PstI CTGCAG 1 cut(s) 1905
PstNI CAGNNNCTG 1 cut(s) 925
PsuI RGATCY 2 cut(s) 540, 832
PvuI CGATCG 1 cut(s) 1568
PvuII CAGCTG 3 cut(s) 1174, 1370, 1516
RsaI GTAC 7 cut(s) 176, 268, 635, 884, 1330, 1476, 2171
RsaNI GTAC 7 cut(s) 175, 267, 634, 883, 1329, 1475, 2170
RseI CAYNNNNRTG 2 cut(s) 1444, 1917
SacI GAGCTC 1 cut(s) 85
SapI GCTCTTC 3 cut(s) 139, 1191, 1548
SaqAI TTAA 5 cut(s) 375, 576, 809, 1398, 1790
SatI GCNGC 4 cut(s) 71, 920, 1517, 1665
Sau96I GGNCC 8 cut(s) 93, 230, 562, 748, 963, 964, 1720, 1928
ScaI AGTACT 2 cut(s) 268, 2171
SchI GAGTC 3 cut(s) 454, 1593, 1622
SduI GDGCHC 6 cut(s) 85, 783, 967, 1636, 2398, 2437
SexAI ACCWGGT 1 cut(s) 636
SfaNI GCATC 5 cut(s) 673, 1054, 1648, 2032, 2313
SfcI CTRYAG 3 cut(s) 652, 714, 1901
SinI GGWCC 5 cut(s) 230, 562, 748, 1720, 1928
SmiI ATTTAAAT 1 cut(s) 1399
SmiMI CAYNNNNRTG 2 cut(s) 1444, 1917
SmlI CTYRAG 2 cut(s) 836, 2309
SmoI CTYRAG 2 cut(s) 836, 2309
SsiI CCGC 6 cut(s) 6, 19, 70, 565, 1852, 2261
SspI AATATT 2 cut(s) 1623, 2441
SspMI CTAG 3 cut(s) 515, 1263, 2226
SstI GAGCTC 1 cut(s) 85
SwaI ATTTAAAT 1 cut(s) 1399
TaaI ACNGT 6 cut(s) 313, 742, 1328, 1446, 2195, 2400
TaiI ACGT 5 cut(s) 490, 604, 1240, 1480, 1859
TaqI TCGA 7 cut(s) 66, 458, 645, 1189, 1194, 1564, 1756
TatI WGTACW 3 cut(s) 174, 266, 2169
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 8 cut(s) 221, 364, 683, 874, 1191, 1561, 1867, 2242
Tru1I TTAA 5 cut(s) 375, 576, 809, 1398, 1790
Tru9I TTAA 5 cut(s) 375, 576, 809, 1398, 1790
TscAI CASTG 5 cut(s) 745, 1129, 1578, 1641, 2403
TseFI GTSAC 3 cut(s) 290, 736, 908
TseI GCWGC 3 cut(s) 919, 1516, 1664
Tsp45I GTSAC 3 cut(s) 290, 736, 908
TspGWI ACGGA 3 cut(s) 149, 180, 223
TspRI CASTG 5 cut(s) 745, 1129, 1578, 1641, 2403
VneI GTGCAC 2 cut(s) 1632, 2394
VpaK11BI GGWCC 5 cut(s) 230, 562, 748, 1720, 1928
XagI CCTNNNNNAGG 1 cut(s) 2298
XapI RAATTY 4 cut(s) 694, 932, 1838, 2324
XcmI CCANNNNNNNNNTGG 1 cut(s) 2072
XmnI GAANNNNTTC 3 cut(s) 14, 1104, 1842
XspI CTAG 3 cut(s) 515, 1263, 2226
ZrmI AGTACT 2 cut(s) 268, 2171
Zsp2I ATGCAT 1 cut(s) 1380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.