FvH4_4g34320
BHLH Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016032
Physical Location & Seq
Forward (+)
32175510 .. 32177953
2444 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34320.t1

Sequence Viewer

Length: 1395 bp
ATGGCTATGAGTTTGAAACGTTTGGAGAGACTAATGGAGGAGTGGCTTAGACCTCTTGTTGAGGCCAAGCAATGGGACTATTGTGTTGTTTGGAAACTGGGTCATGACCCAACTAGGTTTATTAAAATGGTGGGTTGCTGTTGTGGTGGTGGTTATGACTACTGTGCTAATGTTAAGGAGGAAGAAGGAGCTCAGGTTTGCAGAGATGGGTACCTTGAGCATCGTATTAAGACCAAGGCTTGTGTGGCTCTGGCTCAGCTGCCTGATTCAATGCCTCTCTATTCTGGGGTTCATACTGAAGTGATCATTTCAGACCAACCCAGATGGATTAGTAATGCCTTGGATTCAAATCATCCAGAATCACATGACTCGAAAGGAACCCGAGTTTTGATCCCGCTGCTGCTCAGTGGTCTTATTGAGCTCTTCACCACAGACAATCTTCCAGAAAACCAAGAGATGTTAGACTTGCTTATGGAATCCTTTAATGCCAATGTGAACCTTAAAGGCAGTGCAAACCTTGATGAACTTCATATTGACCCAATGAAGGAGAAGTTCTTGCCTAAGGTGTGTCCTAGACCACAGCTTACCTCAAGGACCCAATCTACTTCATGTCCTAGCCGCGAGGGATCATCCAGTGGTTCAAACCCTTCCACTGAACATCGACCATTTAACTTAAGTTCTGATCAACTGCCACCATATGGATATGTGATGCCTTCTGAGAAATCCTCTCGCTTCAGAAAGCCAGAGTTTCGTGACAACTTGTTGAAGCAACAAGTAGTGGAGAAAGATAATGCAAGGGGTGTGGGGAGGACAGGAAGTGAGCATTTCCGTTCCAAGAATCTCAACGCAGAGAGAAAGAGAAGGAATAGAATTAAAGAAGGGGAACTTGCTTTACGTGCTCTAGTGCCAAAGATAACCAAGATGGATAGAGCTTCTATTCCAGCAGATGCTATTGAGTACATAAAAGAGTTGCAGCAGCAGGTGAAAGAACTAGAAGAGGAGCTGAGGGAAATGGAAGAAGAGGACTCTGCAAATAACGAAGTAGATTTACATGTTTCGGCGCTGGATAAGAACAAACGAGGCAGCAGCAGCTTGACTTGTACAGACCACAAGCAGATATCTAGCATTGCCCTAAAAATTCCAACTGAGGTGCATTTGGAGGTGTACCAGCTTAGCAAAAAAGAATGTTTGATCAAGTTGTTCTGTGAGCACAACCGAGGTGGGTTTACAAGGTTGATAGAAACTATGGATTGCTTAGGACTTGAAGTAGTGGATGCCAATGTTACCACATTTGACAGAAATTCCCTAATCATTCTCAGAGTCGAGGCGAATGAGGATATTCAAGCAAAGAAATTGAAAGATTTGCTGATCAAGCTAACAAGGGGGAGAAATTAG

Protein Analysis

465

Amino Acids

52.73

Weight (kDa)

6.36

Isoelectric Point (pI)

50.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 14 - 161 2.6e-15 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 280 - 325 1.1e-06 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 386 - 454 2.6e-08 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016032)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10610 AT1G10610 AT1G10610
fragaria_vesca FvH4_4g34320
malus_domestica MD16G1093900.v1.1
prunus_persica Prupe.1G252600_v2.0.a1
pyrus_communis pycom16g07990
rosa_chinensis RchiOBHm_Chr4g0443741
rosa_laevigata RLG00000005894
rosa_roxburghii Rroxscaffold_5G00384310
rosa_samantha Rh4AG407100 Rh4BG418000 Rh4CG432200 Rh4DG413300
rosa_wichuraiana Rw4G034960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 970
Acc36I ACCTGC 1 cut(s) 970
Acc65I GGTACC 1 cut(s) 210
AccB1I GGYRCC 1 cut(s) 210
AccB7I CCANNNNNTGG 2 cut(s) 72, 698
AccII CGCG 1 cut(s) 621
AciI CCGC 2 cut(s) 395, 619
AclI AACGTT 1 cut(s) 19
AclWI GGATC 2 cut(s) 385, 634
AcsI RAATTY 2 cut(s) 1137, 1300
AcuI CTGAAG 2 cut(s) 318, 718
AfaI GTAC 4 cut(s) 212, 959, 1102, 1166
AfiI CCNNNNNNNGG 3 cut(s) 72, 544, 698
AflII CTTAAG 1 cut(s) 673
AflIII ACRYGT 1 cut(s) 1051
AgsI TTSAA 8 cut(s) 16, 270, 348, 642, 766, 1265, 1343, 1357
AluBI AGCT 9 cut(s) 191, 259, 421, 583, 932, 1003, 1092, 1171, 1375
AluI AGCT 9 cut(s) 191, 259, 421, 583, 932, 1003, 1092, 1171, 1375
Alw21I GWGCWC 4 cut(s) 193, 423, 901, 1212
Alw26I GTCTC 1 cut(s) 22
AlwI GGATC 2 cut(s) 385, 634
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 8 cut(s) 259, 397, 400, 973, 976, 1083, 1086, 1089
ApoI RAATTY 2 cut(s) 1137, 1300
Asp718I GGTACC 1 cut(s) 210
AspLEI GCGC 1 cut(s) 1063
AspS9I GGNCC 1 cut(s) 594
AsuHPI GGTGA 2 cut(s) 418, 994
AvaI CYCGRG 1 cut(s) 381
AvaII GGWCC 1 cut(s) 594
AxyI CCTNAGG 1 cut(s) 561
BanI GGYRCC 1 cut(s) 210
BanII GRGCYC 2 cut(s) 193, 423
BarI GAAGNNNNNNTAC 2 cut(s) 1032, 1064
Bbv12I GWGCWC 4 cut(s) 193, 423, 901, 1212
BbvCI CCTCAGC 1 cut(s) 1004
BbvI GCAGC 8 cut(s) 246, 384, 387, 985, 988, 1095, 1098, 1101
BccI CCATC 3 cut(s) 200, 318, 916
BclI TGATCA 4 cut(s) 303, 682, 1191, 1368
BcoDI GTCTC 1 cut(s) 22
BfaI CTAG 6 cut(s) 114, 573, 615, 902, 992, 1122
BfoI RGCGCY 1 cut(s) 1064
BfrI CTTAAG 1 cut(s) 673
BfuAI ACCTGC 1 cut(s) 970
BisI GCNGC 9 cut(s) 260, 398, 401, 619, 974, 977, 1084, 1087, 1090
BlpI GCTNAGC 2 cut(s) 255, 1172
BlsI GCNGC 9 cut(s) 261, 399, 402, 620, 975, 978, 1085, 1088, 1091
Bme18I GGWCC 1 cut(s) 594
BmeT110I CYCGRG 1 cut(s) 381
BmgT120I GGNCC 1 cut(s) 594
BmiI GGNNCC 3 cut(s) 212, 379, 596
BmrI ACTGGG 1 cut(s) 107
BmsI GCATC 4 cut(s) 229, 699, 937, 1264
BmuI ACTGGG 1 cut(s) 107
BplI GAGNNNNNCTC 2 cut(s) 710, 742
Bpu10I CCTNAGC 3 cut(s) 192, 1004, 1255
Bpu1102I GCTNAGC 2 cut(s) 255, 1172
BpuEI CTTGAG 2 cut(s) 236, 574
BsaAI YACGTR 1 cut(s) 896
BsaBI GATNNNNATC 1 cut(s) 348
BsaJI CCNNGG 3 cut(s) 234, 339, 1216
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 544, 698
Bse1I ACTGG 2 cut(s) 102, 633
Bse21I CCTNAGG 1 cut(s) 561
Bse3DI GCAATG 2 cut(s) 77, 1125
Bse8I GATNNNNATC 1 cut(s) 348
BseDI CCNNGG 3 cut(s) 234, 339, 1216
BseGI GGATG 3 cut(s) 352, 629, 1279
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 3 cut(s) 72, 544, 698
BseMI GCAATG 2 cut(s) 77, 1125
BseMII CTCAG 7 cut(s) 206, 269, 418, 708, 995, 1137, 1330
BseNI ACTGG 2 cut(s) 102, 633
BseRI GAGGAG 2 cut(s) 53, 1013
BseXI GCAGC 8 cut(s) 246, 384, 387, 985, 988, 1095, 1098, 1101
Bsh1236I CGCG 1 cut(s) 621
BshFI GGCC 1 cut(s) 65
BshNI GGYRCC 1 cut(s) 210
BsiHKAI GWGCWC 4 cut(s) 193, 423, 901, 1212
BsiHKCI CYCGRG 1 cut(s) 381
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 3 cut(s) 72, 544, 698
BsmAI GTCTC 1 cut(s) 22
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 1 cut(s) 65
BsoBI CYCGRG 1 cut(s) 381
Bsp1286I GDGCHC 4 cut(s) 193, 423, 901, 1212
Bsp1407I TGTACA 1 cut(s) 1100
Bsp143I GATC 6 cut(s) 303, 390, 626, 682, 1191, 1368
Bsp1720I GCTNAGC 2 cut(s) 255, 1172
BspACI CCGC 2 cut(s) 395, 619
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 7 cut(s) 205, 268, 417, 709, 996, 1138, 1329
BspFNI CGCG 1 cut(s) 621
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 3 cut(s) 212, 379, 596
BspMI ACCTGC 1 cut(s) 970
BspPI GGATC 2 cut(s) 385, 634
BspQI GCTCTTC 1 cut(s) 428
BspT107I GGYRCC 1 cut(s) 210
BspTI CTTAAG 1 cut(s) 673
BsrDI GCAATG 2 cut(s) 77, 1125
BsrGI TGTACA 1 cut(s) 1100
BsrI ACTGG 2 cut(s) 102, 633
BssECI CCNNGG 3 cut(s) 234, 339, 1216
BssMI GATC 6 cut(s) 303, 390, 626, 682, 1191, 1368
BssT1I CCWWGG 2 cut(s) 234, 339
Bst4CI ACNGT 1 cut(s) 164
Bst6I CTCTTC 3 cut(s) 428, 990, 1014
BstAFI CTTAAG 1 cut(s) 673
BstAUI TGTACA 1 cut(s) 1100
BstBAI YACGTR 1 cut(s) 896
BstF5I GGATG 3 cut(s) 352, 629, 1279
BstFNI CGCG 1 cut(s) 621
BstH2I RGCGCY 1 cut(s) 1064
BstHHI GCGC 1 cut(s) 1063
BstKTI GATC 6 cut(s) 306, 393, 629, 685, 1194, 1371
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 6 cut(s) 303, 390, 626, 682, 1191, 1368
BstMWI GCNNNNNNNGC 4 cut(s) 245, 896, 1089, 1372
BstNSI RCATGY 1 cut(s) 1055
BstUI CGCG 1 cut(s) 621
BstV1I GCAGC 8 cut(s) 246, 384, 387, 985, 988, 1095, 1098, 1101
Bsu36I CCTNAGG 1 cut(s) 561
BsuRI GGCC 1 cut(s) 65
BtsCI GGATG 3 cut(s) 352, 629, 1279
BtsI GCAGTG 1 cut(s) 514
BtsIMutI CAGTG 4 cut(s) 412, 514, 640, 651
BveI ACCTGC 1 cut(s) 970
CciI TCATGA 1 cut(s) 103
CfoI GCGC 1 cut(s) 1063
Cfr13I GGNCC 1 cut(s) 594
Csp6I GTAC 4 cut(s) 211, 958, 1101, 1165
CspCI CAANNNNNGTGG 4 cut(s) 783, 818, 1201, 1236
CviAII CATG 4 cut(s) 104, 365, 609, 1052
CviQI GTAC 4 cut(s) 211, 958, 1101, 1165
DpnI GATC 6 cut(s) 305, 392, 628, 684, 1193, 1370
DpnII GATC 6 cut(s) 303, 390, 626, 682, 1191, 1368
Eam1104I CTCTTC 3 cut(s) 428, 990, 1014
EarI CTCTTC 3 cut(s) 428, 990, 1014
Ecl136II GAGCTC 2 cut(s) 191, 421
Eco130I CCWWGG 2 cut(s) 234, 339
Eco24I GRGCYC 2 cut(s) 193, 423
Eco32I GATATC 1 cut(s) 1119
Eco47I GGWCC 1 cut(s) 594
Eco53kI GAGCTC 2 cut(s) 191, 421
Eco57I CTGAAG 2 cut(s) 318, 718
Eco81I CCTNAGG 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 381
EcoICRI GAGCTC 2 cut(s) 191, 421
EcoO109I RGGNCCY 1 cut(s) 594
EcoRV GATATC 1 cut(s) 1119
EcoT14I CCWWGG 2 cut(s) 234, 339
EcoT38I GRGCYC 2 cut(s) 193, 423
ErhI CCWWGG 2 cut(s) 234, 339
FaeI CATG 4 cut(s) 107, 368, 612, 1055
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FaqI GGGAC 1 cut(s) 89
FatI CATG 4 cut(s) 103, 364, 608, 1051
FauI CCCGC 1 cut(s) 402
FauNDI CATATG 1 cut(s) 697
FbaI TGATCA 4 cut(s) 303, 682, 1191, 1368
Fnu4HI GCNGC 9 cut(s) 260, 398, 401, 619, 974, 977, 1084, 1087, 1090
FokI GGATG 3 cut(s) 339, 616, 1286
FriOI GRGCYC 2 cut(s) 193, 423
Fsp4HI GCNGC 9 cut(s) 260, 398, 401, 619, 974, 977, 1084, 1087, 1090
FspBI CTAG 6 cut(s) 114, 573, 615, 902, 992, 1122
GlaI GCGC 1 cut(s) 1062
GluI GCNGC 9 cut(s) 260, 398, 401, 619, 974, 977, 1084, 1087, 1090
HaeII RGCGCY 1 cut(s) 1064
HaeIII GGCC 1 cut(s) 65
HhaI GCGC 1 cut(s) 1063
Hin1II CATG 4 cut(s) 107, 368, 612, 1055
Hin6I GCGC 1 cut(s) 1061
HinP1I GCGC 1 cut(s) 1061
HinfI GANTC 8 cut(s) 266, 344, 359, 368, 476, 838, 1025, 1320
HphI GGTGA 2 cut(s) 418, 994
Hpy166II GTNNAC 3 cut(s) 496, 1165, 1227
Hpy188I TCNGA 5 cut(s) 313, 682, 718, 737, 1319
Hpy188III TCNNGA 4 cut(s) 104, 356, 443, 752
Hpy8I GTNNAC 3 cut(s) 496, 1165, 1227
HpyAV CCTTC 6 cut(s) 179, 538, 657, 723, 855, 872
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4IV ACGT 2 cut(s) 19, 895
HpyCH4V TGCA 6 cut(s) 201, 512, 794, 973, 1031, 1153
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 896, 1089, 1372
HpySE526I ACGT 2 cut(s) 19, 895
Hsp92II CATG 4 cut(s) 107, 368, 612, 1055
HspAI GCGC 1 cut(s) 1061
KpnI GGTACC 1 cut(s) 214
Ksp22I TGATCA 4 cut(s) 303, 682, 1191, 1368
Kzo9I GATC 6 cut(s) 303, 390, 626, 682, 1191, 1368
LguI GCTCTTC 1 cut(s) 428
LmnI GCTCC 2 cut(s) 188, 1000
Lsp1109I GCAGC 8 cut(s) 246, 384, 387, 985, 988, 1095, 1098, 1101
LweI GCATC 4 cut(s) 229, 699, 937, 1264
MaeI CTAG 6 cut(s) 114, 573, 615, 902, 992, 1122
MaeII ACGT 2 cut(s) 19, 895
MaeIII GTNAC 2 cut(s) 752, 1282
MalI GATC 6 cut(s) 305, 392, 628, 684, 1193, 1370
MboI GATC 6 cut(s) 303, 390, 626, 682, 1191, 1368
MboII GAAGA 6 cut(s) 194, 415, 431, 1007, 1028, 1031
MhlI GDGCHC 4 cut(s) 193, 423, 901, 1212
MluCI AATT 5 cut(s) 870, 1137, 1300, 1352, 1390
MlyI GAGTC 3 cut(s) 362, 1019, 1329
MmeI TCCRAC 1 cut(s) 1166
MseI TTAA 8 cut(s) 123, 174, 228, 483, 501, 669, 674, 873
MspA1I CMGCKG 2 cut(s) 259, 397
MspCI CTTAAG 1 cut(s) 673
MvnI CGCG 1 cut(s) 621
MwoI GCNNNNNNNGC 4 cut(s) 245, 896, 1089, 1372
NdeI CATATG 1 cut(s) 697
NdeII GATC 6 cut(s) 303, 390, 626, 682, 1191, 1368
NlaIII CATG 4 cut(s) 107, 368, 612, 1055
NlaIV GGNNCC 3 cut(s) 212, 379, 596
NmuCI GTSAC 1 cut(s) 752
NspI RCATGY 1 cut(s) 1055
PagI TCATGA 1 cut(s) 103
PaqCI CACCTGC 1 cut(s) 970
PciI ACATGT 1 cut(s) 1051
PciSI GCTCTTC 1 cut(s) 428
PfeI GAWTC 5 cut(s) 266, 344, 359, 476, 838
PflMI CCANNNNNTGG 2 cut(s) 72, 698
PkrI GCNGC 9 cut(s) 261, 399, 402, 620, 975, 978, 1085, 1088, 1091
PleI GAGTC 3 cut(s) 362, 1019, 1328
PpsI GAGTC 3 cut(s) 362, 1019, 1328
Ppu21I YACGTR 1 cut(s) 896
PpuMI RGGWCCY 1 cut(s) 594
PscI ACATGT 1 cut(s) 1051
Psp124BI GAGCTC 2 cut(s) 193, 423
Psp1406I AACGTT 1 cut(s) 19
Psp5II RGGWCCY 1 cut(s) 594
PspN4I GGNNCC 3 cut(s) 212, 379, 596
PspPI GGNCC 1 cut(s) 594
PspPPI RGGWCCY 1 cut(s) 594
PsrI GAACNNNNNNTAC 2 cut(s) 876, 908
PvuII CAGCTG 1 cut(s) 259
RsaI GTAC 4 cut(s) 212, 959, 1102, 1166
RsaNI GTAC 4 cut(s) 211, 958, 1101, 1165
SacI GAGCTC 2 cut(s) 193, 423
SapI GCTCTTC 1 cut(s) 428
SaqAI TTAA 8 cut(s) 123, 174, 228, 483, 501, 669, 674, 873
SatI GCNGC 9 cut(s) 260, 398, 401, 619, 974, 977, 1084, 1087, 1090
Sau3AI GATC 6 cut(s) 303, 390, 626, 682, 1191, 1368
Sau96I GGNCC 1 cut(s) 594
SchI GAGTC 3 cut(s) 362, 1019, 1329
SduI GDGCHC 4 cut(s) 193, 423, 901, 1212
SfaNI GCATC 4 cut(s) 229, 699, 937, 1264
SinI GGWCC 1 cut(s) 594
SmlI CTYRAG 3 cut(s) 215, 589, 673
SmoI CTYRAG 3 cut(s) 215, 589, 673
Sse9I AATT 5 cut(s) 870, 1137, 1300, 1352, 1390
SsiI CCGC 2 cut(s) 395, 619
SspMI CTAG 6 cut(s) 114, 573, 615, 902, 992, 1122
SstI GAGCTC 2 cut(s) 193, 423
StyI CCWWGG 2 cut(s) 234, 339
TaaI ACNGT 1 cut(s) 164
TaiI ACGT 2 cut(s) 22, 898
TaqI TCGA 3 cut(s) 371, 661, 1323
TasI AATT 5 cut(s) 870, 1137, 1300, 1352, 1390
TatI WGTACW 2 cut(s) 957, 1100
TauI GCSGC 1 cut(s) 621
TfiI GAWTC 5 cut(s) 266, 344, 359, 476, 838
Tru1I TTAA 8 cut(s) 123, 174, 228, 483, 501, 669, 674, 873
Tru9I TTAA 8 cut(s) 123, 174, 228, 483, 501, 669, 674, 873
TscAI CASTG 4 cut(s) 412, 514, 640, 658
TseFI GTSAC 1 cut(s) 752
TseI GCWGC 8 cut(s) 259, 397, 400, 973, 976, 1083, 1086, 1089
Tsp45I GTSAC 1 cut(s) 752
TspDTI ATGAA 5 cut(s) 281, 518, 537, 557, 597
TspGWI ACGGA 1 cut(s) 818
TspRI CASTG 4 cut(s) 412, 514, 640, 658
Van91I CCANNNNNTGG 2 cut(s) 72, 698
Vha464I CTTAAG 1 cut(s) 673
VpaK11BI GGWCC 1 cut(s) 594
XapI RAATTY 2 cut(s) 1137, 1300
XceI RCATGY 1 cut(s) 1055
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XspI CTAG 6 cut(s) 114, 573, 615, 902, 992, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.