Rw4G034960
BHLH Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016032
Physical Location & Seq
Forward (+)
61859622 .. 61862106
2485 bp
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UTR
Exon/CDS
Intron
Rw4G034960.1

Sequence Viewer

Length: 1464 bp
ATGGCTACGAGTGTGAAACGTTTGGAGAGACTGATGGAGGAGTGGCTTAGACCTCTTGTTGAGGCCGAGCAATGGGACTATTGTGTTGTTTGGAAACTGGGTCATGACCCATCTAGGTTTATTGAAATGGTGGGTTGCTGCTGTGGTGGAGGTTATGACTACTGTGCTAATGTTAAAGAGGAAGAGGGAGTTCAGCTTTGCAGAGATGGGTACCTTGAGCATCGTATTAAGACCAAGGCTTGTGAAGCTCTGGCTCAGCTTCCTGATTCAATGCCTCTTTACTCTGGGGTTCATACTGATGTGGTCATTTCAGACCAACCCAGATGGATTATTAATGCCTTGGAGTCGAATCATTCAGAATCACATGACTTGAATGGAACCCGAGTTTTGATCCCGCTGCTGCTCACTGGTCTTATTGAGCTCTTTACCACAAAGAATATGCCAGAAAACCAAAAGATGTTAGACTTTCTTATGGAATCCTTTAATGTTGCTCTGAAAGAAGAAGTCATGACTAGACATAGCTGTGCTAATGTGAACCTCAAAGCTAGTGCAACCCTTGATGAACTTCATATTGACCCAATGCTGGAGAAGTCTTTGCAAAACTGGTTTCACTCTCCGAATTTGCCTAAGCTGAGTCCTAGGCCACAGATTGTTACCTCAAGAACCCAATCTACTTCATGTCCTAGCCGCGAGGGGTCATCCAGCGGTTCAAACCCTGCCACTGAACATCCAGCATTTAACTTAAGTTCTGCTCAAGTATCACCACATGGCTGTGTGAAGCCTTCTGAGAAATCCTCTTGCTTTAGAAAGCCAAAGTGCTGCGACAACTTGTTGAAGCAACAAGTAGTGGAGAAAGATAATGCTAGGGGTGTGAGGAGGACAGGAAGTGAACATTTCCGTTCCAAGAATCTCAACGCAGAGAGGAAGAGAAGGACTAGAATTAAAGAAGGTGAACTTGCTTTACGTGCTCTAGTGCCAAAGATAACCAAGATGGATAGAGCGTCTATTCCAACAGATGCTATTGACTACATAAAAGAGTTGCAGCAGAAGGTGAAAGAACTAGAAGACGAGCTGAGGGAAATGGAAGAAGAAGACTCTGCAAAGAAGGAAGCAGATTTACAGGTTTCAGTGCTGGACAAGAAGAAAGGAGGCAGTAACTGCTTGAGTACTACAGACCACAAACAGATCTCCAGTTTTTCCCCAAAAAGTCCAACTGAGGTGCATTTGGGGGTGTACCAGCTGAGCAAAAAAGAATGTTTGATTAAGTTGTTCTGTGAGCACAACCGAGGTGGGTTTACAAGGTTGATGGAGACTATGGATTGCTTAGGGCTTGAAGTAGTGGATGCCAACGTCACCACATTTGATAGAAATTCCCTAATCATTCTCAGAGTTGAGGCGAATGAGGATATCCAAGCAAAGAAATTGAGGAACATGCTAATCAAGCTAACAAGGGAGACAAATTAG

Protein Analysis

487

Amino Acids

55.13

Weight (kDa)

6.72

Isoelectric Point (pI)

50.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 14 - 161 6.7e-16 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 303 - 348 1e-06 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 411 - 480 5.2e-09 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016032)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10610 AT1G10610 AT1G10610
fragaria_vesca FvH4_4g34320
malus_domestica MD16G1093900.v1.1
prunus_persica Prupe.1G252600_v2.0.a1
pyrus_communis pycom16g07990
rosa_chinensis RchiOBHm_Chr4g0443741
rosa_laevigata RLG00000005894
rosa_roxburghii Rroxscaffold_5G00384310
rosa_samantha Rh4AG407100 Rh4BG418000 Rh4CG432200 Rh4DG413300
rosa_wichuraiana Rw4G034960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 210
AccB1I GGYRCC 1 cut(s) 210
AccII CGCG 1 cut(s) 690
AciI CCGC 3 cut(s) 395, 688, 705
AclI AACGTT 1 cut(s) 19
AclWI GGATC 1 cut(s) 385
AcsI RAATTY 2 cut(s) 619, 1369
AfaI GTAC 3 cut(s) 212, 1168, 1235
AfiI CCNNNNNNNGG 2 cut(s) 72, 583
AflII CTTAAG 1 cut(s) 742
AgsI TTSAA 6 cut(s) 125, 270, 373, 711, 835, 1334
Alw21I GWGCWC 3 cut(s) 423, 970, 1281
Alw26I GTCTC 3 cut(s) 22, 1304, 1448
AlwI GGATC 1 cut(s) 385
AlwNI CAGNNNCTG 1 cut(s) 1158
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 2 cut(s) 63, 641
ApeKI GCWGC 5 cut(s) 138, 397, 400, 819, 1042
ApoI RAATTY 2 cut(s) 619, 1369
AseI ATTAAT 1 cut(s) 333
Asp718I GGTACC 1 cut(s) 210
AspA2I CCTAGG 1 cut(s) 638
AsuHPI GGTGA 4 cut(s) 753, 962, 1063, 1345
AvaI CYCGRG 1 cut(s) 381
AvrII CCTAGG 1 cut(s) 638
BanI GGYRCC 1 cut(s) 210
BanII GRGCYC 1 cut(s) 423
BarI GAAGNNNNNNTAC 2 cut(s) 1101, 1133
BbsI GAAGAC 2 cut(s) 1071, 1098
Bbv12I GWGCWC 3 cut(s) 423, 970, 1281
BbvCI CCTCAGC 1 cut(s) 1073
BbvI GCAGC 5 cut(s) 125, 384, 387, 806, 1054
BccI CCATC 6 cut(s) 28, 118, 200, 318, 985, 1300
BcoDI GTCTC 3 cut(s) 22, 1304, 1448
BfaI CTAG 9 cut(s) 114, 513, 546, 639, 684, 864, 936, 971, 1061
BfmI CTRYAG 1 cut(s) 1170
BfrI CTTAAG 1 cut(s) 742
BglII AGATCT 1 cut(s) 1185
BisI GCNGC 6 cut(s) 139, 398, 401, 688, 820, 1043
BlnI CCTAGG 1 cut(s) 638
BlpI GCTNAGC 2 cut(s) 255, 1241
BlsI GCNGC 6 cut(s) 140, 399, 402, 689, 821, 1044
BmcAI AGTACT 1 cut(s) 1168
BmeT110I CYCGRG 1 cut(s) 381
BmiI GGNNCC 2 cut(s) 212, 379
BmrI ACTGGG 1 cut(s) 107
BmsI GCATC 3 cut(s) 229, 1006, 1333
BmuI ACTGGG 1 cut(s) 107
BpiI GAAGAC 2 cut(s) 1071, 1098
BplI GAGNNNNNCTC 2 cut(s) 779, 811
BpmI CTGGAG 2 cut(s) 605, 1174
Bpu10I CCTNAGC 3 cut(s) 627, 1073, 1324
Bpu1102I GCTNAGC 2 cut(s) 255, 1241
BpuEI CTTGAG 4 cut(s) 236, 643, 738, 1183
BsaAI YACGTR 1 cut(s) 965
BsaJI CCNNGG 4 cut(s) 234, 339, 638, 1285
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 583
Bse1I ACTGG 4 cut(s) 102, 412, 608, 1191
Bse3DI GCAATG 1 cut(s) 77
BseDI CCNNGG 4 cut(s) 234, 339, 638, 1285
BseGI GGATG 3 cut(s) 698, 727, 1348
BseLI CCNNNNNNNGG 2 cut(s) 72, 583
BseMI GCAATG 1 cut(s) 77
BseMII CTCAG 7 cut(s) 269, 623, 777, 1064, 1206, 1232, 1399
BseNI ACTGG 4 cut(s) 102, 412, 608, 1191
BseRI GAGGAG 2 cut(s) 53, 889
BseXI GCAGC 5 cut(s) 125, 384, 387, 806, 1054
Bsh1236I CGCG 1 cut(s) 690
BshFI GGCC 2 cut(s) 65, 643
BshNI GGYRCC 1 cut(s) 210
BsiHKAI GWGCWC 3 cut(s) 423, 970, 1281
BsiHKCI CYCGRG 1 cut(s) 381
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 2 cut(s) 72, 583
BsmAI GTCTC 3 cut(s) 22, 1304, 1448
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 2 cut(s) 65, 643
BsoBI CYCGRG 1 cut(s) 381
Bsp1286I GDGCHC 3 cut(s) 423, 970, 1281
Bsp143I GATC 2 cut(s) 390, 1185
Bsp1720I GCTNAGC 2 cut(s) 255, 1241
BspACI CCGC 3 cut(s) 395, 688, 705
BspANI GGCC 2 cut(s) 65, 643
BspCNI CTCAG 7 cut(s) 268, 624, 778, 1065, 1207, 1233, 1398
BspFNI CGCG 1 cut(s) 690
BspHI TCATGA 2 cut(s) 103, 507
BspLI GGNNCC 2 cut(s) 212, 379
BspPI GGATC 1 cut(s) 385
BspT107I GGYRCC 1 cut(s) 210
BspTI CTTAAG 1 cut(s) 742
BsrDI GCAATG 1 cut(s) 77
BsrI ACTGG 4 cut(s) 102, 412, 608, 1191
BssECI CCNNGG 4 cut(s) 234, 339, 638, 1285
BssMI GATC 2 cut(s) 390, 1185
BssT1I CCWWGG 3 cut(s) 234, 339, 638
Bst4CI ACNGT 1 cut(s) 164
Bst6I CTCTTC 2 cut(s) 177, 920
BstAFI CTTAAG 1 cut(s) 742
BstAPI GCANNNNNTGC 1 cut(s) 1158
BstBAI YACGTR 1 cut(s) 965
BstF5I GGATG 3 cut(s) 698, 727, 1348
BstFNI CGCG 1 cut(s) 690
BstKTI GATC 2 cut(s) 393, 1188
BstMAI GTCTC 3 cut(s) 22, 1304, 1448
BstMBI GATC 2 cut(s) 390, 1185
BstMWI GCNNNNNNNGC 4 cut(s) 245, 965, 1158, 1441
BstNSI RCATGY 1 cut(s) 1435
BstSFI CTRYAG 1 cut(s) 1170
BstUI CGCG 1 cut(s) 690
BstV1I GCAGC 5 cut(s) 125, 384, 387, 806, 1054
BstV2I GAAGAC 2 cut(s) 1071, 1098
BstX2I RGATCY 1 cut(s) 1185
BstYI RGATCY 1 cut(s) 1185
BsuRI GGCC 2 cut(s) 65, 643
BtsCI GGATG 3 cut(s) 698, 727, 1348
BtsIMutI CAGTG 3 cut(s) 405, 720, 1134
CaiI CAGNNNCTG 1 cut(s) 1158
CciI TCATGA 2 cut(s) 103, 507
CseI GACGC 1 cut(s) 990
Csp6I GTAC 3 cut(s) 211, 1167, 1234
CspCI CAANNNNNGTGG 2 cut(s) 1270, 1305
CviAII CATG 6 cut(s) 104, 365, 508, 678, 767, 1432
CviQI GTAC 3 cut(s) 211, 1167, 1234
DpnI GATC 2 cut(s) 392, 1187
DpnII GATC 2 cut(s) 390, 1185
Eam1104I CTCTTC 2 cut(s) 177, 920
EarI CTCTTC 2 cut(s) 177, 920
Ecl136II GAGCTC 1 cut(s) 421
Eco130I CCWWGG 3 cut(s) 234, 339, 638
Eco24I GRGCYC 1 cut(s) 423
Eco32I GATATC 1 cut(s) 1408
Eco53kI GAGCTC 1 cut(s) 421
Eco88I CYCGRG 1 cut(s) 381
EcoICRI GAGCTC 1 cut(s) 421
EcoRV GATATC 1 cut(s) 1408
EcoT14I CCWWGG 3 cut(s) 234, 339, 638
EcoT38I GRGCYC 1 cut(s) 423
ErhI CCWWGG 3 cut(s) 234, 339, 638
FaeI CATG 6 cut(s) 107, 368, 511, 681, 770, 1435
FalI AAGNNNNNCTT 2 cut(s) 939, 971
FaqI GGGAC 1 cut(s) 89
FatI CATG 6 cut(s) 103, 364, 507, 677, 766, 1431
FauI CCCGC 1 cut(s) 402
Fnu4HI GCNGC 6 cut(s) 139, 398, 401, 688, 820, 1043
FokI GGATG 3 cut(s) 685, 714, 1355
FriOI GRGCYC 1 cut(s) 423
Fsp4HI GCNGC 6 cut(s) 139, 398, 401, 688, 820, 1043
FspBI CTAG 9 cut(s) 114, 513, 546, 639, 684, 864, 936, 971, 1061
GluI GCNGC 6 cut(s) 139, 398, 401, 688, 820, 1043
GsuI CTGGAG 2 cut(s) 605, 1174
HaeIII GGCC 2 cut(s) 65, 643
HgaI GACGC 1 cut(s) 990
Hin1II CATG 6 cut(s) 107, 368, 511, 681, 770, 1435
HinfI GANTC 8 cut(s) 266, 344, 349, 359, 476, 634, 907, 1094
HphI GGTGA 4 cut(s) 753, 962, 1063, 1345
Hpy166II GTNNAC 5 cut(s) 535, 890, 953, 1234, 1296
Hpy188I TCNGA 6 cut(s) 313, 358, 495, 618, 787, 1388
Hpy188III TCNNGA 4 cut(s) 104, 263, 508, 660
Hpy8I GTNNAC 5 cut(s) 535, 890, 953, 1234, 1296
HpyAV CCTTC 5 cut(s) 792, 924, 941, 1042, 1099
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4IV ACGT 3 cut(s) 19, 964, 1350
HpyCH4V TGCA 6 cut(s) 201, 551, 598, 1042, 1100, 1222
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 965, 1158, 1441
HpySE526I ACGT 3 cut(s) 19, 964, 1350
Hsp92II CATG 6 cut(s) 107, 368, 511, 681, 770, 1435
KpnI GGTACC 1 cut(s) 214
Kzo9I GATC 2 cut(s) 390, 1185
Lsp1109I GCAGC 5 cut(s) 125, 384, 387, 806, 1054
LweI GCATC 3 cut(s) 229, 1006, 1333
MaeI CTAG 9 cut(s) 114, 513, 546, 639, 684, 864, 936, 971, 1061
MaeII ACGT 3 cut(s) 19, 964, 1350
MaeIII GTNAC 3 cut(s) 652, 1154, 1351
MalI GATC 2 cut(s) 392, 1187
MboI GATC 2 cut(s) 390, 1185
MboII GAAGA 8 cut(s) 194, 512, 937, 1076, 1097, 1100, 1103, 1153
MflI RGATCY 1 cut(s) 1185
MhlI GDGCHC 3 cut(s) 423, 970, 1281
MluCI AATT 5 cut(s) 619, 939, 1369, 1421, 1459
MlyI GAGTC 3 cut(s) 353, 643, 1088
MmeI TCCRAC 2 cut(s) 1034, 1235
MseI TTAA 8 cut(s) 174, 228, 333, 483, 738, 743, 942, 1263
MslI CAYNNNNRTG 3 cut(s) 297, 522, 771
MspA1I CMGCKG 3 cut(s) 397, 705, 1240
MspCI CTTAAG 1 cut(s) 742
MvnI CGCG 1 cut(s) 690
MwoI GCNNNNNNNGC 4 cut(s) 245, 965, 1158, 1441
NdeII GATC 2 cut(s) 390, 1185
NlaIII CATG 6 cut(s) 107, 368, 511, 681, 770, 1435
NlaIV GGNNCC 2 cut(s) 212, 379
NmeAIII GCCGAG 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 1351
NspI RCATGY 1 cut(s) 1435
PagI TCATGA 2 cut(s) 103, 507
PfeI GAWTC 5 cut(s) 266, 349, 359, 476, 907
PkrI GCNGC 6 cut(s) 140, 399, 402, 689, 821, 1044
PleI GAGTC 3 cut(s) 352, 642, 1088
PpsI GAGTC 3 cut(s) 352, 642, 1088
Ppu21I YACGTR 1 cut(s) 965
PshBI ATTAAT 1 cut(s) 333
Psp124BI GAGCTC 1 cut(s) 423
Psp1406I AACGTT 1 cut(s) 19
PspN4I GGNNCC 2 cut(s) 212, 379
PsrI GAACNNNNNNTAC 4 cut(s) 655, 687, 945, 977
PstNI CAGNNNCTG 1 cut(s) 1158
PsuI RGATCY 1 cut(s) 1185
PvuII CAGCTG 1 cut(s) 1240
RsaI GTAC 3 cut(s) 212, 1168, 1235
RsaNI GTAC 3 cut(s) 211, 1167, 1234
RseI CAYNNNNRTG 3 cut(s) 297, 522, 771
SacI GAGCTC 1 cut(s) 423
SaqAI TTAA 8 cut(s) 174, 228, 333, 483, 738, 743, 942, 1263
SatI GCNGC 6 cut(s) 139, 398, 401, 688, 820, 1043
Sau3AI GATC 2 cut(s) 390, 1185
ScaI AGTACT 1 cut(s) 1168
SchI GAGTC 3 cut(s) 353, 643, 1088
SduI GDGCHC 3 cut(s) 423, 970, 1281
SfaNI GCATC 3 cut(s) 229, 1006, 1333
SfcI CTRYAG 1 cut(s) 1170
SmiMI CAYNNNNRTG 3 cut(s) 297, 522, 771
SmlI CTYRAG 5 cut(s) 215, 658, 742, 753, 1162
SmoI CTYRAG 5 cut(s) 215, 658, 742, 753, 1162
Sse9I AATT 5 cut(s) 619, 939, 1369, 1421, 1459
SsiI CCGC 3 cut(s) 395, 688, 705
SspMI CTAG 9 cut(s) 114, 513, 546, 639, 684, 864, 936, 971, 1061
SstI GAGCTC 1 cut(s) 423
StyI CCWWGG 3 cut(s) 234, 339, 638
TaaI ACNGT 1 cut(s) 164
TaiI ACGT 3 cut(s) 22, 967, 1353
TaqI TCGA 1 cut(s) 347
TasI AATT 5 cut(s) 619, 939, 1369, 1421, 1459
TatI WGTACW 1 cut(s) 1166
TauI GCSGC 1 cut(s) 690
TfiI GAWTC 5 cut(s) 266, 349, 359, 476, 907
Tru1I TTAA 8 cut(s) 174, 228, 333, 483, 738, 743, 942, 1263
Tru9I TTAA 8 cut(s) 174, 228, 333, 483, 738, 743, 942, 1263
TscAI CASTG 3 cut(s) 412, 727, 1134
TseFI GTSAC 1 cut(s) 1351
TseI GCWGC 5 cut(s) 138, 397, 400, 819, 1042
Tsp45I GTSAC 1 cut(s) 1351
TspDTI ATGAA 4 cut(s) 281, 557, 576, 666
TspGWI ACGGA 1 cut(s) 887
TspRI CASTG 3 cut(s) 412, 727, 1134
Vha464I CTTAAG 1 cut(s) 742
VspI ATTAAT 1 cut(s) 333
XapI RAATTY 2 cut(s) 619, 1369
XceI RCATGY 1 cut(s) 1435
XmaJI CCTAGG 1 cut(s) 638
XspI CTAG 9 cut(s) 114, 513, 546, 639, 684, 864, 936, 971, 1061
ZrmI AGTACT 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.