FvH4_4g36400

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018839
Physical Location & Seq
Forward (+)
33365263 .. 33366616
1354 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36400.t1

Sequence Viewer

Length: 846 bp
ATGGCAAAAGCTATAACGGAATTTGTGAGAACACATTCTTCTGATGATCCTACTGAAAGACAAGAAGCCGCATGCAATGGAGATCAACAAAAACAAGAACACAACGTGGAGGAGGATAAGGCGAAAAAGAAGAAAACAAAAGGTGCTGATCATAATGCAGCAGCAGCTGCCCCCCAACAGCCCTACCCATTTCTAATTCCTGCTTCCGCCACTACTTCTAGTATTGAGGCTAAAGTTGAAGACCCTAGTAGTGTTTTCGAAAGTCTGTTAGCAGATCTTGAAGGTCAATCCGAAAAGAATGTCGAAGACAATGGTAGTCTTTGCAAATTGCTAGAGATCGAAACAGTTCAAGCGCAAAATGCTACCAACGGAAGGTCATCTAACAAGCTCGCAAGGTCATTGTCTAGGAGTTTCTCCTTCAAATTTCTTTCGTCAGGGTGGAGGAGTCGCTCCTCCAAAGTTCGTTGTTCACCAAGTGATGCCGAAGACGGGCATTCTTCAACACTAAAGTACTTATGTGCTTGGGAGAAACAACTCTATTACCTCGTGGAGAGGGTACTAGACGAGGAAAAAACAGGAGGATTTCATGAAGTAAACGTTGCGAAACAAAGCACTGTATCGGCATTTAACCAAATCAAACAGCTGAAAGATCAAAAGCTAAGGCCTCATCTTGATCAGCTCCTACGTAGTCTGACACTTAATTGGAAGAAAAATTCGGATTGCTACTCAGCTCAAGTCCAGATCATGTCTCAAATAATAGTGAAATATACTCGGCCTCGTAGACTGTGTTCCGATGTCCCCCACTGGCGTGCTGCTAAGAAACTTAAAGCACAACTCGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

282

Amino Acids

31.59

Weight (kDa)

8.76

Isoelectric Point (pI)

58.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 88 - 183 5.5e-08 Protein of unknown function (DUF632)
DUF632 PF04782 198 - 280 2.5e-08 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018839)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36400 FvH4_4g36610 FvH4_4g36631
prunus_persica Prupe.5G138800_v2.0.a1
rosa_laevigata RLG00000005688
rosa_roxburghii Rroxscaffold_5G00386540
rosa_samantha Rh4AG429900 Rh4BG439300 Rh4CG456600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 781
AciI CCGC 2 cut(s) 69, 207
AclI AACGTT 1 cut(s) 597
AclWI GGATC 1 cut(s) 41
AcsI RAATTY 3 cut(s) 20, 422, 712
AdeI CACNNNGTG 2 cut(s) 106, 476
AfaI GTAC 2 cut(s) 512, 558
AfiI CCNNNNNNNGG 2 cut(s) 372, 489
AgsI TTSAA 5 cut(s) 239, 281, 350, 421, 501
AleI CACNNNNGTG 1 cut(s) 807
AluBI AGCT 7 cut(s) 11, 167, 388, 643, 658, 679, 731
AluI AGCT 7 cut(s) 11, 167, 388, 643, 658, 679, 731
Alw26I GTCTC 1 cut(s) 753
AlwI GGATC 1 cut(s) 41
AlwNI CAGNNNCTG 1 cut(s) 167
AoxI GGCC 2 cut(s) 662, 773
ApeKI GCWGC 5 cut(s) 158, 161, 164, 167, 812
ApoI RAATTY 3 cut(s) 20, 422, 712
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
Asp700I GAANNNNTTC 2 cut(s) 34, 345
AspLEI GCGC 1 cut(s) 355
AsuHPI GGTGA 1 cut(s) 462
AsuII TTCGAA 1 cut(s) 258
BauI CACGAG 1 cut(s) 545
BbsI GAAGAC 3 cut(s) 246, 312, 492
BbvI GCAGC 5 cut(s) 154, 170, 173, 176, 799
BclI TGATCA 2 cut(s) 148, 673
BcoDI GTCTC 1 cut(s) 753
BfaI CTAG 5 cut(s) 219, 246, 332, 405, 560
BglII AGATCT 1 cut(s) 274
BisI GCNGC 6 cut(s) 69, 159, 162, 165, 168, 813
BlsI GCNGC 6 cut(s) 70, 160, 163, 166, 169, 814
BmcAI AGTACT 1 cut(s) 512
BmsI GCATC 1 cut(s) 469
BpiI GAAGAC 3 cut(s) 246, 312, 492
Bpu10I CCTNAGC 1 cut(s) 659
Bpu14I TTCGAA 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 717
BsaAI YACGTR 1 cut(s) 686
Bsc4I CCNNNNNNNGG 2 cut(s) 372, 489
Bse1I ACTGG 1 cut(s) 809
Bse3DI GCAATG 1 cut(s) 82
BseLI CCNNNNNNNGG 2 cut(s) 372, 489
BseMI GCAATG 1 cut(s) 82
BseMII CTCAG 1 cut(s) 741
BseNI ACTGG 1 cut(s) 809
BseRI GAGGAG 3 cut(s) 125, 442, 457
BseXI GCAGC 5 cut(s) 154, 170, 173, 176, 799
BshFI GGCC 2 cut(s) 664, 775
BslFI GGGAC 1 cut(s) 782
BslI CCNNNNNNNGG 2 cut(s) 372, 489
BsmAI GTCTC 1 cut(s) 753
BsmFI GGGAC 1 cut(s) 782
BsmI GAATGC 1 cut(s) 493
BsnI GGCC 2 cut(s) 664, 775
Bsp119I TTCGAA 1 cut(s) 258
Bsp143I GATC 8 cut(s) 46, 82, 148, 274, 336, 649, 673, 741
BspACI CCGC 2 cut(s) 69, 207
BspANI GGCC 2 cut(s) 664, 775
BspCNI CTCAG 1 cut(s) 740
BspHI TCATGA 1 cut(s) 586
BspPI GGATC 1 cut(s) 41
BspT104I TTCGAA 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 82
BsrI ACTGG 1 cut(s) 809
BssMI GATC 8 cut(s) 46, 82, 148, 274, 336, 649, 673, 741
BssSI CACGAG 1 cut(s) 545
Bst2BI CACGAG 1 cut(s) 545
Bst4CI ACNGT 3 cut(s) 346, 616, 786
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBAI YACGTR 1 cut(s) 686
BstBI TTCGAA 1 cut(s) 258
BstC8I GCNNGC 3 cut(s) 73, 390, 810
BstDEI CTNAG 3 cut(s) 659, 727, 816
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 8 cut(s) 49, 85, 151, 277, 339, 652, 676, 744
BstMAI GTCTC 1 cut(s) 753
BstMBI GATC 8 cut(s) 46, 82, 148, 274, 336, 649, 673, 741
BstMWI GCNNNNNNNGC 3 cut(s) 164, 167, 359
BstNSI RCATGY 1 cut(s) 75
BstSNI TACGTA 1 cut(s) 686
BstV1I GCAGC 5 cut(s) 154, 170, 173, 176, 799
BstV2I GAAGAC 3 cut(s) 246, 312, 492
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BsuRI GGCC 2 cut(s) 664, 775
BtsIMutI CAGTG 2 cut(s) 612, 802
Cac8I GCNNGC 3 cut(s) 73, 390, 810
CaiI CAGNNNCTG 1 cut(s) 167
CciI TCATGA 1 cut(s) 586
CfoI GCGC 1 cut(s) 355
Csp6I GTAC 2 cut(s) 511, 557
CviAII CATG 3 cut(s) 72, 587, 745
CviQI GTAC 2 cut(s) 511, 557
DdeI CTNAG 3 cut(s) 659, 727, 816
DpnI GATC 8 cut(s) 48, 84, 150, 276, 338, 651, 675, 743
DpnII GATC 8 cut(s) 46, 82, 148, 274, 336, 649, 673, 741
DraIII CACNNNGTG 2 cut(s) 106, 476
EciI GGCGGA 1 cut(s) 196
Eco105I TACGTA 1 cut(s) 686
Eco147I AGGCCT 1 cut(s) 664
FaeI CATG 3 cut(s) 75, 590, 748
FaiI YATR 7 cut(s) 14, 73, 153, 517, 588, 746, 768
FaqI GGGAC 1 cut(s) 782
FatI CATG 3 cut(s) 71, 586, 744
FbaI TGATCA 2 cut(s) 148, 673
FblI GTMKAC 1 cut(s) 781
Fnu4HI GCNGC 6 cut(s) 69, 159, 162, 165, 168, 813
Fsp4HI GCNGC 6 cut(s) 69, 159, 162, 165, 168, 813
FspBI CTAG 5 cut(s) 219, 246, 332, 405, 560
GlaI GCGC 1 cut(s) 354
GluI GCNGC 6 cut(s) 69, 159, 162, 165, 168, 813
HaeIII GGCC 2 cut(s) 664, 775
HhaI GCGC 1 cut(s) 355
Hin1II CATG 3 cut(s) 75, 590, 748
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HinfI GANTC 1 cut(s) 445
HphI GGTGA 1 cut(s) 462
Hpy166II GTNNAC 3 cut(s) 470, 595, 782
Hpy188I TCNGA 5 cut(s) 43, 292, 693, 718, 793
Hpy188III TCNNGA 4 cut(s) 278, 587, 671, 739
Hpy8I GTNNAC 3 cut(s) 470, 595, 782
HpyAV CCTTC 3 cut(s) 275, 366, 427
HpyCH4III ACNGT 3 cut(s) 346, 616, 786
HpyCH4IV ACGT 3 cut(s) 105, 597, 685
HpyCH4V TGCA 3 cut(s) 75, 158, 324
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 167, 359
HpyF3I CTNAG 3 cut(s) 659, 727, 816
HpySE526I ACGT 3 cut(s) 105, 597, 685
Hsp92II CATG 3 cut(s) 75, 590, 748
HspAI GCGC 1 cut(s) 353
Ksp22I TGATCA 2 cut(s) 148, 673
Kzo9I GATC 8 cut(s) 46, 82, 148, 274, 336, 649, 673, 741
LmnI GCTCC 2 cut(s) 455, 684
LpnPI CCDG 5 cut(s) 213, 420, 561, 752, 790
Lsp1109I GCAGC 5 cut(s) 154, 170, 173, 176, 799
LweI GCATC 1 cut(s) 469
MaeI CTAG 5 cut(s) 219, 246, 332, 405, 560
MaeII ACGT 3 cut(s) 105, 597, 685
MalI GATC 8 cut(s) 48, 84, 150, 276, 338, 651, 675, 743
MboI GATC 8 cut(s) 46, 82, 148, 274, 336, 649, 673, 741
MboII GAAGA 7 cut(s) 30, 142, 251, 317, 489, 497, 718
MflI RGATCY 1 cut(s) 274
MluCI AATT 6 cut(s) 20, 195, 326, 422, 700, 712
MlyI GAGTC 1 cut(s) 454
MroXI GAANNNNTTC 2 cut(s) 34, 345
MseI TTAA 3 cut(s) 627, 699, 825
MslI CAYNNNNRTG 1 cut(s) 807
MspA1I CMGCKG 2 cut(s) 167, 643
Mva1269I GAATGC 1 cut(s) 493
MwoI GCNNNNNNNGC 3 cut(s) 164, 167, 359
NdeII GATC 8 cut(s) 46, 82, 148, 274, 336, 649, 673, 741
NlaIII CATG 3 cut(s) 75, 590, 748
NmeAIII GCCGAG 1 cut(s) 751
NspI RCATGY 1 cut(s) 75
NspV TTCGAA 1 cut(s) 258
OliI CACNNNNGTG 1 cut(s) 807
PaeI GCATGC 1 cut(s) 75
PagI TCATGA 1 cut(s) 586
PceI AGGCCT 1 cut(s) 664
PctI GAATGC 1 cut(s) 493
PdmI GAANNNNTTC 2 cut(s) 34, 345
PkrI GCNGC 6 cut(s) 70, 160, 163, 166, 169, 814
PleI GAGTC 1 cut(s) 453
PpsI GAGTC 1 cut(s) 453
Ppu21I YACGTR 1 cut(s) 686
Psp1406I AACGTT 1 cut(s) 597
PsrI GAACNNNNNNTAC 2 cut(s) 772, 804
PstNI CAGNNNCTG 1 cut(s) 167
PsuI RGATCY 1 cut(s) 274
PvuII CAGCTG 2 cut(s) 167, 643
RsaI GTAC 2 cut(s) 512, 558
RsaNI GTAC 2 cut(s) 511, 557
RseI CAYNNNNRTG 1 cut(s) 807
SaqAI TTAA 3 cut(s) 627, 699, 825
SatI GCNGC 6 cut(s) 69, 159, 162, 165, 168, 813
Sau3AI GATC 8 cut(s) 46, 82, 148, 274, 336, 649, 673, 741
ScaI AGTACT 1 cut(s) 512
SchI GAGTC 1 cut(s) 454
SfaNI GCATC 1 cut(s) 469
SfuI TTCGAA 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 807
SmlI CTYRAG 1 cut(s) 732
SmoI CTYRAG 1 cut(s) 732
SnaBI TACGTA 1 cut(s) 686
SphI GCATGC 1 cut(s) 75
Sse9I AATT 6 cut(s) 20, 195, 326, 422, 700, 712
SseBI AGGCCT 1 cut(s) 664
SsiI CCGC 2 cut(s) 69, 207
SspMI CTAG 5 cut(s) 219, 246, 332, 405, 560
StuI AGGCCT 1 cut(s) 664
TaaI ACNGT 3 cut(s) 346, 616, 786
TaiI ACGT 3 cut(s) 108, 600, 688
TaqI TCGA 4 cut(s) 258, 303, 339, 837
TasI AATT 6 cut(s) 20, 195, 326, 422, 700, 712
TatI WGTACW 1 cut(s) 510
TauI GCSGC 1 cut(s) 71
Tru1I TTAA 3 cut(s) 627, 699, 825
Tru9I TTAA 3 cut(s) 627, 699, 825
TscAI CASTG 2 cut(s) 619, 809
TseI GCWGC 5 cut(s) 158, 161, 164, 167, 812
TspDTI ATGAA 2 cut(s) 575, 603
TspGWI ACGGA 2 cut(s) 32, 384
TspRI CASTG 2 cut(s) 619, 809
XapI RAATTY 3 cut(s) 20, 422, 712
XceI RCATGY 1 cut(s) 75
XmiI GTMKAC 1 cut(s) 781
XmnI GAANNNNTTC 2 cut(s) 34, 345
XspI CTAG 5 cut(s) 219, 246, 332, 405, 560
ZrmI AGTACT 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.