Rh4AG429900

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0018839
Physical Location & Seq
Forward (+)
72521396 .. 72528829
7434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG429900.1

Sequence Viewer

Length: 429 bp
ATGTGGTGGATCATGGTGGAATGCCTTGGTGGCGTACGTGTCCTTAGCCCAATTGATCGAGATCATGATTCATTCTCCAAAAATAGTGAAGTCGCTCCTCCAAAGGTCGTTGTTTACCAAAGTGATGAGAAAGACGGGAAGCATTCTTCAACACTGAAGTACTTAGGTGCTTGGGATAAACAACCCTATTACCTCGTGGAGAAAGTACTCGAAGAGGAGAAAATAGGTGGAGTCCTTGAAGTAAACGTTGCCAATCAAAGCGCTGTATCAGCATTAAACCAAATTCGACAGCTGAGAGATCAAGAGCTACGGCCTCAACTTGATAAGCTTCTACGTGGTCTGACACAAAATTGGGTGAAAATTTCGGAATGCTATGCAACTCAAGCCATGATCATGTCTGAAATAATAGTGAAATCTCTCTCTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

142

Amino Acids

16.16

Weight (kDa)

5.71

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 81 - 135 1.1e-11 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018839)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36400 FvH4_4g36610 FvH4_4g36631
prunus_persica Prupe.5G138800_v2.0.a1
rosa_laevigata RLG00000005688
rosa_roxburghii Rroxscaffold_5G00386540
rosa_samantha Rh4AG429900 Rh4BG439300 Rh4CG456600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 246
AclWI GGATC 1 cut(s) 17
AcsI RAATTY 2 cut(s) 282, 360
AcuI CTGAAG 1 cut(s) 176
AfaI GTAC 3 cut(s) 36, 161, 207
AfeI AGCGCT 1 cut(s) 262
AflIII ACRYGT 1 cut(s) 37
AgsI TTSAA 2 cut(s) 150, 239
AluBI AGCT 3 cut(s) 292, 307, 328
AluI AGCT 3 cut(s) 292, 307, 328
AlwI GGATC 1 cut(s) 17
Aor51HI AGCGCT 1 cut(s) 262
AoxI GGCC 1 cut(s) 311
ApoI RAATTY 2 cut(s) 282, 360
AspLEI GCGC 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 367
BauI CACGAG 1 cut(s) 194
BceAI ACGGC 1 cut(s) 326
BclI TGATCA 1 cut(s) 390
BfoI RGCGCY 1 cut(s) 264
BglI GCCNNNNNGGC 1 cut(s) 30
BmcAI AGTACT 2 cut(s) 161, 207
Bpu10I CCTNAGC 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 366
BsaAI YACGTR 2 cut(s) 38, 335
BsaBI GATNNNNATC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 25
Bse8I GATNNNNATC 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 25
BseJI GATNNNNATC 1 cut(s) 60
BseMII CTCAG 1 cut(s) 284
BseRI GAGGAG 2 cut(s) 87, 230
BshFI GGCC 1 cut(s) 313
BsiWI CGTACG 1 cut(s) 34
BsmI GAATGC 3 cut(s) 26, 142, 374
BsnI GGCC 1 cut(s) 313
Bsp143I GATC 5 cut(s) 9, 55, 61, 298, 390
BspANI GGCC 1 cut(s) 313
BspCNI CTCAG 1 cut(s) 285
BspHI TCATGA 1 cut(s) 64
BspPI GGATC 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 25
BssMI GATC 5 cut(s) 9, 55, 61, 298, 390
BssSI CACGAG 1 cut(s) 194
BssT1I CCWWGG 1 cut(s) 25
Bst2BI CACGAG 1 cut(s) 194
Bst6I CTCTTC 1 cut(s) 207
BstBAI YACGTR 2 cut(s) 38, 335
BstDEI CTNAG 3 cut(s) 44, 163, 293
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 5 cut(s) 12, 58, 64, 301, 393
BstMBI GATC 5 cut(s) 9, 55, 61, 298, 390
BstMWI GCNNNNNNNGC 3 cut(s) 30, 269, 383
BsuRI GGCC 1 cut(s) 313
BtsIMutI CAGTG 1 cut(s) 152
CciI TCATGA 1 cut(s) 64
CfoI GCGC 1 cut(s) 263
Csp6I GTAC 3 cut(s) 35, 160, 206
CviAII CATG 4 cut(s) 13, 65, 388, 394
CviJI RGCY 6 cut(s) 48, 292, 307, 313, 328, 386
CviKI_1 RGCY 6 cut(s) 48, 292, 307, 313, 328, 386
CviQI GTAC 3 cut(s) 35, 160, 206
DdeI CTNAG 3 cut(s) 44, 163, 293
DpnI GATC 5 cut(s) 11, 57, 63, 300, 392
DpnII GATC 5 cut(s) 9, 55, 61, 298, 390
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 25
Eco47III AGCGCT 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 25
FaeI CATG 4 cut(s) 16, 68, 391, 397
FaiI YATR 7 cut(s) 14, 66, 375, 389, 395, 425, 427
FatI CATG 4 cut(s) 12, 64, 387, 393
FbaI TGATCA 1 cut(s) 390
GlaI GCGC 1 cut(s) 262
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 1 cut(s) 313
HhaI GCGC 1 cut(s) 263
Hin1II CATG 4 cut(s) 16, 68, 391, 397
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 2 cut(s) 68, 231
HphI GGTGA 1 cut(s) 367
Hpy166II GTNNAC 2 cut(s) 115, 244
Hpy188I TCNGA 3 cut(s) 342, 367, 400
Hpy188III TCNNGA 3 cut(s) 59, 65, 302
Hpy8I GTNNAC 2 cut(s) 115, 244
HpyCH4IV ACGT 3 cut(s) 37, 246, 334
HpyCH4V TGCA 1 cut(s) 377
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 269, 383
HpyF3I CTNAG 3 cut(s) 44, 163, 293
HpySE526I ACGT 3 cut(s) 37, 246, 334
Hsp92II CATG 4 cut(s) 16, 68, 391, 397
HspAI GCGC 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 5 cut(s) 9, 55, 61, 298, 390
LmnI GCTCC 1 cut(s) 100
MaeII ACGT 3 cut(s) 37, 246, 334
MalI GATC 5 cut(s) 11, 57, 63, 300, 392
MboI GATC 5 cut(s) 9, 55, 61, 298, 390
MboII GAAGA 2 cut(s) 138, 224
MfeI CAATTG 1 cut(s) 51
MluCI AATT 4 cut(s) 51, 282, 349, 360
MlyI GAGTC 1 cut(s) 240
MnlI CCTC 4 cut(s) 108, 203, 208, 324
MseI TTAA 1 cut(s) 275
MslI CAYNNNNRTG 1 cut(s) 392
MspA1I CMGCKG 1 cut(s) 292
MunI CAATTG 1 cut(s) 51
Mva1269I GAATGC 3 cut(s) 26, 142, 374
MwoI GCNNNNNNNGC 3 cut(s) 30, 269, 383
NdeII GATC 5 cut(s) 9, 55, 61, 298, 390
NlaIII CATG 4 cut(s) 16, 68, 391, 397
PagI TCATGA 1 cut(s) 64
PctI GAATGC 3 cut(s) 26, 142, 374
PfeI GAWTC 1 cut(s) 68
Pfl23II CGTACG 1 cut(s) 34
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
Ppu21I YACGTR 2 cut(s) 38, 335
Psp1406I AACGTT 1 cut(s) 246
PspLI CGTACG 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 292
RsaI GTAC 3 cut(s) 36, 161, 207
RsaNI GTAC 3 cut(s) 35, 160, 206
RseI CAYNNNNRTG 1 cut(s) 392
SaqAI TTAA 1 cut(s) 275
Sau3AI GATC 5 cut(s) 9, 55, 61, 298, 390
ScaI AGTACT 2 cut(s) 161, 207
SchI GAGTC 1 cut(s) 240
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 381
SmoI CTYRAG 1 cut(s) 381
Sse9I AATT 4 cut(s) 51, 282, 349, 360
StyI CCWWGG 1 cut(s) 25
TaiI ACGT 3 cut(s) 40, 249, 337
TaqI TCGA 3 cut(s) 58, 210, 286
TasI AATT 4 cut(s) 51, 282, 349, 360
TatI WGTACW 2 cut(s) 159, 205
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TscAI CASTG 1 cut(s) 159
TspDTI ATGAA 1 cut(s) 60
TspRI CASTG 1 cut(s) 159
XapI RAATTY 2 cut(s) 282, 360
ZrmI AGTACT 2 cut(s) 161, 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.