FvH4_4g36610

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018839
Physical Location & Seq
Reverse (-)
33473782 .. 33475263
1482 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36610.t1

Sequence Viewer

Length: 1482 bp
ATGAAAGGCGTTCCTGCTTCTTTGTCTAGGAGAGGCGTTAGCAAAGAGAAATCCGGTAAAGATGCTTGTATTGAGAGAATAAAGTTGACTGAATTGGCTTTACAGCGCTGGAAGGACTTTGCTGCTGCATACAAGACATACAATGATCTGCAGAGCACAATGGCAAAAGCTATAACGGAATTTGTGAGAACACATTCTTCTGATGATCCTGCTGAAAGACAAGAAGCCACATGCAATGGAGATCAACAAAAACAAGAACACAAGGTGGAGGAGGATCCGAATAAGGCGAAAAAGAAGAAAACAAAAGGTGCTGATAATGCAGCAGCAGCTGGTAACAGCACACATGCTTCTAGTATTGAGGCTAAAGTTGAAGACCCTAGTAGTGTTTTCGAAAGTCTGTTAGCAGATCTTGAAGGTCAATCCGAAAAGAATGTCGAAGACAATGGTAGTCTTTGCAAATTGCTAGAGATTGACACGGTTCAAACGCAAACTACTACCAAAGCAAGCTCATCAAACAAGCTGCTAGCAAGGTCATTGTCTAGGAGTTTCTCCTTCAAATTTCTTTCGTCAGCGTGGAGGAGTCGCTCCTCCAAAGTTCGTTGTTCACCAAGTGATGCCGAAGACGGGCATTCTTCAACACTAAAGTACTTATGTGCTTGGGAGAAACAACTCCATTACCTCGTGGAGAGGGTACTAGACGAGGAAAAAACAGGTGGACTTCATGAAGTAAACGTTGCAAAACAAAGCACTGTATTGTCATTTAAGCAAATCCAATATCTGAAAGATCAAAAGCTAAGACCTCATCTTGATCAGCTCCTACGTAGTCTGACACTTAATTGGAAGAAAAATTCGGATTGCTACTCAGCTCAAGTGCAGATCATGTCTCAAACAATAGTGAAATATACTCGGCCTCGTAGACTGTGTTCCGATGTCCCCCACTGGCGTGCTGCTAAGAAACTTAAAGCACAACTCGAAGAATGGCTTGCATGCTTTGCTAATTATGTTTCTTCGCACAAGGCCTACATTAAGGCTCTTCATGATTGGGCAAGGCATCCGCTCCCGGCGTTCTGTGAAGATAACAACGACAACAGCAACAACATCTACGCGCTACCGCTGCTTGATAAGTGGTCAAGTTGTTTGGATAAGCTGTTTGCTGATGTGACTGTGACGAAAGCCATAGGTGACTTGGCCACAAAAGTTGGAGAACTAGTGGAGTTGCAAAATAAGGAATATGAACACAAGAGCCAGGTTGATCAACTCAAAGGGGAGCAAAGGAAAATTGAGCAGTCCAGAAAGGCCAAGGACAAGTCTGATAAGTTAACGCAGAGGATAAAAGAAGAGGAAGTGCAATATGAAGCAAGTCGGAAGGAGACCGAAGAGAATGCACTGAATCTAATTCAGAAGGGGTTTACTTCACTTTTTGGATCCTTGGATGAATTCTCGAAGTCTTTTGCTCAAAAGTATGATGACATTAGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

494

Amino Acids

55.9

Weight (kDa)

8.8

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 132 - 240 1.5e-06 Protein of unknown function (DUF632)
DUF632 PF04782 243 - 401 2.8e-23 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018839)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36400 FvH4_4g36610 FvH4_4g36631
prunus_persica Prupe.5G138800_v2.0.a1
rosa_laevigata RLG00000005688
rosa_roxburghii Rroxscaffold_5G00386540
rosa_samantha Rh4AG429900 Rh4BG439300 Rh4CG456600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1057
AccI GTMKAC 1 cut(s) 916
AccII CGCG 1 cut(s) 1106
AciI CCGC 2 cut(s) 1055, 1112
AclI AACGTT 1 cut(s) 732
AclWI GGATC 5 cut(s) 200, 269, 282, 1419, 1432
AcoI YGGCCR 1 cut(s) 1188
AcsI RAATTY 4 cut(s) 179, 557, 847, 1436
AdeI CACNNNGTG 2 cut(s) 265, 611
AfaI GTAC 2 cut(s) 647, 693
AfeI AGCGCT 1 cut(s) 107
AfiI CCNNNNNNNGG 1 cut(s) 624
AgsI TTSAA 5 cut(s) 371, 413, 482, 556, 636
AhlI ACTAGT 1 cut(s) 1207
AjnI CCWGG 1 cut(s) 1245
AleI CACNNNNGTG 1 cut(s) 942
AluBI AGCT 8 cut(s) 170, 329, 507, 520, 793, 814, 866, 1147
AluI AGCT 8 cut(s) 170, 329, 507, 520, 793, 814, 866, 1147
Alw21I GWGCWC 1 cut(s) 158
Alw26I GTCTC 2 cut(s) 888, 1364
AlwI GGATC 5 cut(s) 200, 269, 282, 1419, 1432
AlwNI CAGNNNCTG 1 cut(s) 329
Aor51HI AGCGCT 1 cut(s) 107
AoxI GGCC 4 cut(s) 908, 1017, 1188, 1296
ApeKI GCWGC 8 cut(s) 122, 125, 320, 323, 326, 520, 947, 1114
ApoI RAATTY 4 cut(s) 179, 557, 847, 1436
ArsI GACNNNNNNTTYG 2 cut(s) 417, 449
Asp700I GAANNNNTTC 1 cut(s) 193
AspLEI GCGC 2 cut(s) 108, 1108
AsuC2I CCSGG 1 cut(s) 1061
AsuHPI GGTGA 2 cut(s) 597, 1193
AsuII TTCGAA 1 cut(s) 390
AsuNHI GCTAGC 1 cut(s) 523
BalI TGGCCA 1 cut(s) 1190
BamHI GGATCC 2 cut(s) 274, 1424
BauI CACGAG 1 cut(s) 680
BbsI GAAGAC 3 cut(s) 378, 444, 627
Bbv12I GWGCWC 1 cut(s) 158
BbvI GCAGC 8 cut(s) 109, 112, 332, 335, 338, 507, 934, 1101
BcgI CGANNNNNNTGC 2 cut(s) 1364, 1398
BciT130I CCWGG 1 cut(s) 1247
BclI TGATCA 2 cut(s) 808, 1252
BcnI CCSGG 1 cut(s) 1061
BcoDI GTCTC 2 cut(s) 888, 1364
BcuI ACTAGT 1 cut(s) 1207
BfaI CTAG 8 cut(s) 27, 351, 378, 464, 524, 540, 695, 1208
BfmI CTRYAG 1 cut(s) 149
BfoI RGCGCY 1 cut(s) 109
BglII AGATCT 1 cut(s) 406
BisI GCNGC 8 cut(s) 123, 126, 321, 324, 327, 521, 948, 1115
BlsI GCNGC 8 cut(s) 124, 127, 322, 325, 328, 522, 949, 1116
BmcAI AGTACT 1 cut(s) 647
Bme1390I CCNGG 2 cut(s) 1061, 1247
BmiI GGNNCC 2 cut(s) 276, 1426
BmrFI CCNGG 2 cut(s) 1061, 1247
BmsI GCATC 3 cut(s) 52, 604, 1060
BmtI GCTAGC 1 cut(s) 527
BpiI GAAGAC 3 cut(s) 378, 444, 627
Bpu14I TTCGAA 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 852
BpuMI CCSGG 1 cut(s) 1061
BsaAI YACGTR 1 cut(s) 821
BsaI GGTCTC 1 cut(s) 1364
BsaJI CCNNGG 2 cut(s) 1299, 1428
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 1194, 1224
Bsc4I CCNNNNNNNGG 1 cut(s) 624
Bse1I ACTGG 1 cut(s) 944
Bse3DI GCAATG 1 cut(s) 241
BseBI CCWGG 1 cut(s) 1247
BseDI CCNNGG 2 cut(s) 1299, 1428
BseGI GGATG 2 cut(s) 1051, 1438
BseLI CCNNNNNNNGG 1 cut(s) 624
BseMI GCAATG 1 cut(s) 241
BseMII CTCAG 1 cut(s) 876
BseNI ACTGG 1 cut(s) 944
BseRI GAGGAG 3 cut(s) 284, 577, 592
BseXI GCAGC 8 cut(s) 109, 112, 332, 335, 338, 507, 934, 1101
BsgI GTGCAG 1 cut(s) 893
Bsh1236I CGCG 1 cut(s) 1106
BshFI GGCC 4 cut(s) 910, 1019, 1190, 1298
BsiHKAI GWGCWC 1 cut(s) 158
BsiSI CCGG 2 cut(s) 54, 1061
BslFI GGGAC 1 cut(s) 917
BslI CCNNNNNNNGG 1 cut(s) 624
BsmAI GTCTC 2 cut(s) 888, 1364
BsmFI GGGAC 1 cut(s) 917
BsmI GAATGC 2 cut(s) 628, 1387
BsnI GGCC 4 cut(s) 910, 1019, 1190, 1298
Bso31I GGTCTC 1 cut(s) 1364
Bsp119I TTCGAA 1 cut(s) 390
Bsp1286I GDGCHC 1 cut(s) 158
BspACI CCGC 2 cut(s) 1055, 1112
BspANI GGCC 4 cut(s) 910, 1019, 1190, 1298
BspCNI CTCAG 1 cut(s) 875
BspFNI CGCG 1 cut(s) 1106
BspHI TCATGA 2 cut(s) 721, 1036
BspLI GGNNCC 2 cut(s) 276, 1426
BspMAI CTGCAG 1 cut(s) 153
BspOI GCTAGC 1 cut(s) 527
BspPI GGATC 5 cut(s) 200, 269, 282, 1419, 1432
BspQI GCTCTTC 1 cut(s) 1038
BspT104I TTCGAA 1 cut(s) 390
BspTNI GGTCTC 1 cut(s) 1364
BsrBI CCGCTC 1 cut(s) 1057
BsrDI GCAATG 1 cut(s) 241
BsrI ACTGG 1 cut(s) 944
BssECI CCNNGG 2 cut(s) 1299, 1428
BssSI CACGAG 1 cut(s) 680
BssT1I CCWWGG 2 cut(s) 1299, 1428
Bst2BI CACGAG 1 cut(s) 680
Bst2UI CCWGG 1 cut(s) 1247
Bst4CI ACNGT 4 cut(s) 478, 751, 921, 1165
Bst6I CTCTTC 3 cut(s) 1038, 1332, 1371
BstAPI GCANNNNNTGC 1 cut(s) 992
BstBAI YACGTR 1 cut(s) 821
BstBI TTCGAA 1 cut(s) 390
BstC8I GCNNGC 5 cut(s) 505, 525, 945, 984, 988
BstDEI CTNAG 3 cut(s) 794, 862, 951
BstF5I GGATG 2 cut(s) 1051, 1438
BstFNI CGCG 1 cut(s) 1106
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 2 cut(s) 108, 1108
BstMAI GTCTC 2 cut(s) 888, 1364
BstMWI GCNNNNNNNGC 4 cut(s) 317, 326, 992, 1114
BstNI CCWGG 1 cut(s) 1247
BstNSI RCATGY 3 cut(s) 234, 347, 990
BstSCI CCNGG 2 cut(s) 1059, 1245
BstSFI CTRYAG 1 cut(s) 149
BstSNI TACGTA 1 cut(s) 821
BstUI CGCG 1 cut(s) 1106
BstV1I GCAGC 8 cut(s) 109, 112, 332, 335, 338, 507, 934, 1101
BstV2I GAAGAC 3 cut(s) 378, 444, 627
BstX2I RGATCY 3 cut(s) 274, 406, 1424
BstYI RGATCY 3 cut(s) 274, 406, 1424
BsuRI GGCC 4 cut(s) 910, 1019, 1190, 1298
BtsCI GGATG 2 cut(s) 1051, 1438
BtsIMutI CAGTG 3 cut(s) 747, 937, 1385
Cac8I GCNNGC 5 cut(s) 505, 525, 945, 984, 988
CaiI CAGNNNCTG 1 cut(s) 329
CciI TCATGA 2 cut(s) 721, 1036
CfoI GCGC 2 cut(s) 108, 1108
Csp6I GTAC 2 cut(s) 646, 692
CspCI CAANNNNNGTGG 2 cut(s) 1180, 1215
CviAII CATG 6 cut(s) 231, 344, 722, 880, 987, 1037
CviQI GTAC 2 cut(s) 646, 692
DdeI CTNAG 3 cut(s) 794, 862, 951
DraIII CACNNNGTG 2 cut(s) 265, 611
EaeI YGGCCR 1 cut(s) 1188
Eam1104I CTCTTC 3 cut(s) 1038, 1332, 1371
EarI CTCTTC 3 cut(s) 1038, 1332, 1371
Eco105I TACGTA 1 cut(s) 821
Eco130I CCWWGG 2 cut(s) 1299, 1428
Eco147I AGGCCT 1 cut(s) 1019
Eco31I GGTCTC 1 cut(s) 1364
Eco47III AGCGCT 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 1436
EcoRII CCWGG 1 cut(s) 1245
EcoT14I CCWWGG 2 cut(s) 1299, 1428
ErhI CCWWGG 2 cut(s) 1299, 1428
FaeI CATG 6 cut(s) 234, 347, 725, 883, 990, 1040
FalI AAGNNNNNCTT 2 cut(s) 966, 998
FaqI GGGAC 1 cut(s) 917
FatI CATG 6 cut(s) 230, 343, 721, 879, 986, 1036
FbaI TGATCA 2 cut(s) 808, 1252
FblI GTMKAC 1 cut(s) 916
Fnu4HI GCNGC 8 cut(s) 123, 126, 321, 324, 327, 521, 948, 1115
FokI GGATG 2 cut(s) 1038, 1445
Fsp4HI GCNGC 8 cut(s) 123, 126, 321, 324, 327, 521, 948, 1115
FspBI CTAG 8 cut(s) 27, 351, 378, 464, 524, 540, 695, 1208
GlaI GCGC 2 cut(s) 107, 1107
GluI GCNGC 8 cut(s) 123, 126, 321, 324, 327, 521, 948, 1115
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 4 cut(s) 910, 1019, 1190, 1298
HapII CCGG 2 cut(s) 54, 1061
HhaI GCGC 2 cut(s) 108, 1108
Hin1II CATG 6 cut(s) 234, 347, 725, 883, 990, 1040
Hin6I GCGC 2 cut(s) 106, 1106
HinP1I GCGC 2 cut(s) 106, 1106
HincII GTYRAC 2 cut(s) 87, 1320
HindII GTYRAC 2 cut(s) 87, 1320
HinfI GANTC 2 cut(s) 580, 1390
HpaI GTTAAC 1 cut(s) 1320
HpaII CCGG 2 cut(s) 54, 1061
HphI GGTGA 2 cut(s) 597, 1193
Hpy166II GTNNAC 7 cut(s) 87, 605, 716, 730, 917, 1320, 1410
Hpy188III TCNNGA 6 cut(s) 410, 722, 806, 1037, 1290, 1441
Hpy8I GTNNAC 7 cut(s) 87, 605, 716, 730, 917, 1320, 1410
HpyAV CCTTC 5 cut(s) 106, 407, 562, 1360, 1396
HpyCH4III ACNGT 4 cut(s) 478, 751, 921, 1165
HpyCH4IV ACGT 2 cut(s) 732, 820
HpyF10VI GCNNNNNNNGC 4 cut(s) 317, 326, 992, 1114
HpyF3I CTNAG 3 cut(s) 794, 862, 951
HpySE526I ACGT 2 cut(s) 732, 820
Hsp92II CATG 6 cut(s) 234, 347, 725, 883, 990, 1040
HspAI GCGC 2 cut(s) 106, 1106
Ksp22I TGATCA 2 cut(s) 808, 1252
KspAI GTTAAC 1 cut(s) 1320
LguI GCTCTTC 1 cut(s) 1038
LmnI GCTCC 4 cut(s) 590, 819, 1062, 1267
Lsp1109I GCAGC 8 cut(s) 109, 112, 332, 335, 338, 507, 934, 1101
LweI GCATC 3 cut(s) 52, 604, 1060
MaeI CTAG 8 cut(s) 27, 351, 378, 464, 524, 540, 695, 1208
MaeII ACGT 2 cut(s) 732, 820
MaeIII GTNAC 4 cut(s) 332, 1159, 1165, 1181
MbiI CCGCTC 1 cut(s) 1057
MflI RGATCY 3 cut(s) 274, 406, 1424
MhlI GDGCHC 1 cut(s) 158
MlsI TGGCCA 1 cut(s) 1190
MluNI TGGCCA 1 cut(s) 1190
MlyI GAGTC 1 cut(s) 589
MmeI TCCRAC 2 cut(s) 1180, 1343
Mox20I TGGCCA 1 cut(s) 1190
MroXI GAANNNNTTC 1 cut(s) 193
MscI TGGCCA 1 cut(s) 1190
MseI TTAA 5 cut(s) 762, 834, 960, 1026, 1319
MslI CAYNNNNRTG 1 cut(s) 942
Msp20I TGGCCA 1 cut(s) 1190
MspA1I CMGCKG 2 cut(s) 329, 1114
MspI CCGG 2 cut(s) 54, 1061
MspR9I CCNGG 2 cut(s) 1061, 1247
Mva1269I GAATGC 2 cut(s) 628, 1387
MvaI CCWGG 1 cut(s) 1247
MvnI CGCG 1 cut(s) 1106
MwoI GCNNNNNNNGC 4 cut(s) 317, 326, 992, 1114
NciI CCSGG 1 cut(s) 1061
NheI GCTAGC 1 cut(s) 523
NlaIII CATG 6 cut(s) 234, 347, 725, 883, 990, 1040
NlaIV GGNNCC 2 cut(s) 276, 1426
NmeAIII GCCGAG 1 cut(s) 886
NmuCI GTSAC 3 cut(s) 1159, 1165, 1181
NspI RCATGY 3 cut(s) 234, 347, 990
NspV TTCGAA 1 cut(s) 390
OliI CACNNNNGTG 1 cut(s) 942
PaeI GCATGC 1 cut(s) 990
PagI TCATGA 2 cut(s) 721, 1036
PceI AGGCCT 1 cut(s) 1019
PciSI GCTCTTC 1 cut(s) 1038
PctI GAATGC 2 cut(s) 628, 1387
PdmI GAANNNNTTC 1 cut(s) 193
PfeI GAWTC 1 cut(s) 1390
PkrI GCNGC 8 cut(s) 124, 127, 322, 325, 328, 522, 949, 1116
PleI GAGTC 1 cut(s) 588
PpsI GAGTC 1 cut(s) 588
Ppu21I YACGTR 1 cut(s) 821
Psp1406I AACGTT 1 cut(s) 732
Psp6I CCWGG 1 cut(s) 1245
PspGI CCWGG 1 cut(s) 1245
PspN4I GGNNCC 2 cut(s) 276, 1426
PsrI GAACNNNNNNTAC 2 cut(s) 907, 939
PstI CTGCAG 1 cut(s) 153
PstNI CAGNNNCTG 1 cut(s) 329
PsuI RGATCY 3 cut(s) 274, 406, 1424
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 2 cut(s) 647, 693
RsaNI GTAC 2 cut(s) 646, 692
RseI CAYNNNNRTG 1 cut(s) 942
SapI GCTCTTC 1 cut(s) 1038
SaqAI TTAA 5 cut(s) 762, 834, 960, 1026, 1319
SatI GCNGC 8 cut(s) 123, 126, 321, 324, 327, 521, 948, 1115
ScaI AGTACT 1 cut(s) 647
SchI GAGTC 1 cut(s) 589
ScrFI CCNGG 2 cut(s) 1061, 1247
SduI GDGCHC 1 cut(s) 158
SfaNI GCATC 3 cut(s) 52, 604, 1060
SfcI CTRYAG 1 cut(s) 149
SfuI TTCGAA 1 cut(s) 390
SmiMI CAYNNNNRTG 1 cut(s) 942
SmlI CTYRAG 1 cut(s) 867
SmoI CTYRAG 1 cut(s) 867
SnaBI TACGTA 1 cut(s) 821
SpeI ACTAGT 1 cut(s) 1207
SphI GCATGC 1 cut(s) 990
SseBI AGGCCT 1 cut(s) 1019
SsiI CCGC 2 cut(s) 1055, 1112
SspMI CTAG 8 cut(s) 27, 351, 378, 464, 524, 540, 695, 1208
StuI AGGCCT 1 cut(s) 1019
StyD4I CCNGG 2 cut(s) 1059, 1245
StyI CCWWGG 2 cut(s) 1299, 1428
TaaI ACNGT 4 cut(s) 478, 751, 921, 1165
TaiI ACGT 2 cut(s) 735, 823
TaqI TCGA 4 cut(s) 390, 435, 972, 1442
TaqII GACCGA 1 cut(s) 1388
TatI WGTACW 1 cut(s) 645
TfiI GAWTC 1 cut(s) 1390
Tru1I TTAA 5 cut(s) 762, 834, 960, 1026, 1319
Tru9I TTAA 5 cut(s) 762, 834, 960, 1026, 1319
TscAI CASTG 3 cut(s) 754, 944, 1392
TseFI GTSAC 3 cut(s) 1159, 1165, 1181
TseI GCWGC 8 cut(s) 122, 125, 320, 323, 326, 520, 947, 1114
Tsp45I GTSAC 3 cut(s) 1159, 1165, 1181
TspDTI ATGAA 7 cut(s) 17, 710, 738, 1025, 1248, 1368, 1449
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 3 cut(s) 754, 944, 1392
XapI RAATTY 4 cut(s) 179, 557, 847, 1436
XceI RCATGY 3 cut(s) 234, 347, 990
XcmI CCANNNNNNNNNTGG 1 cut(s) 1183
XmiI GTMKAC 1 cut(s) 916
XmnI GAANNNNTTC 1 cut(s) 193
XspI CTAG 8 cut(s) 27, 351, 378, 464, 524, 540, 695, 1208
ZrmI AGTACT 1 cut(s) 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.