FvH4_4g36771

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009991
Physical Location & Seq
Reverse (-)
33540030 .. 33545464
5435 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36771.t2

Sequence Viewer

Length: 2682 bp
ATGCGGAGACAAATAGAACCGACTGGTGGAAGAATAACCAGAAGGTCGCTGGGTACGGAAACTGCAAAAGTGAAGTGTGATGTTGATAATGCGGTTGGTGCAGTTCCCTGGATTGGAGATTTCAGCTCAGTAAATGATAGTGCTGCTTTGGAGGAGACTGAGAAAACTAGGCATGATGGTGCATCGTTCTGCACGCTTAAAGAATATCAGTTGTTTGACTCTTTCTTCTCAAAGTTCATGACTACTGCGAGAGACTTCTTCCTGCCTTCTGAAAGGCATAAATTTGGTGTGGTCTCAGAAAGATCAATGCTCTCAGCTCTTGGTATTGGAGATTCCAGTTCGTGGTTGGCAGTGTTATACTTTGCTGGATGCCCCAGTTGTTCGAAGATTATCAATAAAGAGGGTGAACTTAATAATGCTTTAAAGATGGATAATTCAGTTGTTAAGGAGCTCGAGGGTGATAGCAATGCTTTGGAGCCAGCTTTGCCTGCAGACCAGCCATCAGTTCTCTTATTTGTGGATAGATCATCTGACTTGTTAGAGACTAAAATAAACGGGAAGGAAGCGCTTGATGCTCTTCGAGAGTTGGCACTGCACCATCACATGTCTCAACAGAAAGGCAGTCATAGTTGGGACATGCATGAGAAATTCTCTGTCCAAGATAATCAAGCATTAAGAATTACATCCGGACATCCTAAAGTAAAACTATCTCAGACTGCTCAGATTAGCAAACAAAAGGACAAAAGGTCTACTTTCACTATATTAAGTGAGGGCAAACAAGTCACTGTGGAGAAGATGGCTTTGGATCTAAAGGGTAATTCCTTGCAGGATATCTTGGAAATGGTACTTAAGCAAAACAAGAAATCTAAATTAAGCTCACTAGTGAAGGAGTTGGGTTTCCAACTTTTGTCAGATGATATGGACATCAAACCAGCAAATACGTTGCCAGAACAAAAAGAAACTGAATCTGATCTAGTTACAGAAGAGCCATCTAAGGAGGGCCTTGCTACACGTAGTATCGACTCAGACAGAGATCAACTGCTGGATGCAACGATTATATCTACTGAACAGCATCCTGAAACATCTACTGAAAAGCATCCTGAAACGTCATCTCATAACAATGAAGATAAAACAGTTTATGTTGATACAAGCAATCAAATGTCATCTATAGATAGTGAACAACATCTTGCAAATCATAAACATGGAGATTTCTCTGAAGAAGATTCACTGGGCGAAAAGTTTGCAGAGCAGGAGCTTCCATTTCAAGGCTTTAAGGGATCATTTTTCTTTTCTGATGGTAATTATCGGTTACTTCAAGCGCTGACTGGTAGGCCAAAAGTTCCATCCCTGGTAATAGTTGATCCCAAAATGCAGCAGCATTATGTCTTCGCGGAGGGGACTAATTTCAATTATTCATCACTGGTTGATTTTATTTCTGCCTTTCTTAATGGAAGTCTTCTTCCATATCAACAGTCTGAAACTGTACTTAAAAACTCTAGGAAAGCCACTCAACCACCATTTGTTAATCTGGACTTCCGTCAGGTGGATTCTATCCCTCGAGTGACAACTAACACTTTCTCTGAGCTGGTTGTTGGGTTCAATCAATCTGACAGTGATGCTTGGAATAAGGATGTCTTGGTTCTTTTTAGCAATAGATGGTGTGGGTTTTGCCAGCGAATGGAGTTGGTTTTTCATGAAGTATACCGAGCTATGAAGGGTTATGCCAAGATGCTGAAAAGTGAATCAAAGAATGAGAAATCAATGTTTCAAAATGGCAATTTGAAGAATGAATTGCTTAAGCTGCCATTGATGTACTTACTGGATTGCACATCAAATGATTGCAATTTGATACTGAAATCAATGAATCAGAGGGAGGTATATCCTATTCTGGTGCTCTTTCCAGCAGAAAAGAAGCATGCTCTTCCTTATGAAGGAGATATGGCAGTGACTGAGGTTTTCAAGTTCATGGCTGATCATGGAAGTAACAATCATCATCTTGTCAGTGAAAAAGGGATCTTGTGGACTGTAGCTGAAAAGGGACGAAGAAATCAAGATTTCTTTTCAGTTCAGTCATACGACATTCATGAGCAGTCTAGGGACTCACTTCATGAAGTCCTATTAACAAATGTGCATAAACCATTTATTGAAGACAAGCTGGTCAAGTCTCAAATAAGCCAAACCTTGCATGAAGCACCCCCAAATGTGGTGGTCGGTTCCATCCTAGTTGCTACTGATAAACTTCTTGGCGTGCATCCCTTTGATAAATCAGAGATTCTCATTCTGAAGGCAGATCAAGTTAATGGATTTCAAGGTTTAATTATCAACAAGCATATCAGATGGGATGCTCTGCCTGAATTGGGAGAAGAAGTTAAGATATTAGCAGAAGCCCCTTTGTCCTTTGGAGGACCGCTTATAAAGGGTGGAATGCCTCTCGTCGCGTTAACTCAAAAATTTGTCAAGCATGAATATCCGGAAATCTTACCAGGCATTGCATTCCTTGATCCATCAGCAACAATTCAAAAAATCAAAGAGCTCAAGTTAGGAAATCAACCGGTCGCTGACTACTGGTTTTTCTTTGGGTATTCGAGTTGGGGTTGGGACCAGCTCTTTGATGAGATTGATCAAGGAGCTTGGAATTTAAGTGATGACGGTATGCAACATTTAAATTGGCCATCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179 Thioredoxin

Protein Analysis

894

Amino Acids

100.49

Weight (kDa)

5.48

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 952 - 1082 1e-11 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2414
AasI GACNNNNNNGTC 1 cut(s) 2156
AccB7I CCANNNNNTGG 2 cut(s) 342, 1678
AccI GTMKAC 2 cut(s) 749, 1701
AccII CGCG 2 cut(s) 1391, 2438
AccIII TCCGGA 2 cut(s) 686, 2470
AciI CCGC 4 cut(s) 4, 92, 1391, 2408
AclWI GGATC 5 cut(s) 813, 1285, 1355, 2021, 2495
AcoI YGGCCR 1 cut(s) 2669
AcsI RAATTY 4 cut(s) 281, 647, 2450, 2635
AcuI CTGAAG 2 cut(s) 1236, 2303
AfaI GTAC 4 cut(s) 55, 846, 1485, 1814
AfeI AGCGCT 2 cut(s) 567, 1320
AfiI CCNNNNNNNGG 9 cut(s) 26, 113, 342, 1265, 1543, 1678, 1931, 2203, 2357
AflII CTTAAG 2 cut(s) 848, 1796
AflIII ACRYGT 2 cut(s) 603, 1010
AgeI ACCGGT 1 cut(s) 2551
AhdI GACNNNNNGTC 1 cut(s) 745
AhlI ACTAGT 1 cut(s) 880
AjnI CCWGG 3 cut(s) 107, 1347, 2482
AjuI GAANNNNNNNTTGG 2 cut(s) 785, 817
Alw21I GWGCWC 3 cut(s) 453, 1896, 2535
Alw26I GTCTC 6 cut(s) 149, 246, 298, 536, 612, 2169
AlwI GGATC 5 cut(s) 813, 1285, 1355, 2021, 2495
AlwNI CAGNNNCTG 1 cut(s) 1949
Ama87I CYCGRG 2 cut(s) 452, 1557
Aor13HI TCCGGA 2 cut(s) 686, 2470
Aor51HI AGCGCT 2 cut(s) 567, 1320
AoxI GGCC 3 cut(s) 1000, 1331, 2669
ApeKI GCWGC 4 cut(s) 143, 1372, 1375, 1801
ApoI RAATTY 4 cut(s) 281, 647, 2450, 2635
ArsI GACNNNNNNTTYG 2 cut(s) 2591, 2623
AsiGI ACCGGT 1 cut(s) 2551
AspLEI GCGC 2 cut(s) 568, 1321
AspS9I GGNCC 3 cut(s) 1000, 2405, 2599
AsuHPI GGTGA 2 cut(s) 416, 470
AsuII TTCGAA 1 cut(s) 383
AvaI CYCGRG 2 cut(s) 452, 1557
AvaII GGWCC 2 cut(s) 2405, 2599
BalI TGGCCA 1 cut(s) 2671
BanII GRGCYC 2 cut(s) 453, 2535
BbsI GAAGAC 3 cut(s) 1378, 1448, 2154
Bbv12I GWGCWC 3 cut(s) 453, 1896, 2535
BbvI GCAGC 4 cut(s) 130, 1384, 1387, 1788
BcgI CGANNNNNNTGC 2 cut(s) 1223, 1257
BciT130I CCWGG 3 cut(s) 109, 1349, 2484
BclI TGATCA 2 cut(s) 1972, 2620
BcoDI GTCTC 6 cut(s) 149, 246, 298, 536, 612, 2169
BcuI ACTAGT 1 cut(s) 880
BfaI CTAG 7 cut(s) 168, 881, 974, 1497, 2094, 2222, 2676
BfmI CTRYAG 3 cut(s) 489, 1167, 2025
BfoI RGCGCY 2 cut(s) 569, 1322
BfrI CTTAAG 2 cut(s) 848, 1796
BisI GCNGC 4 cut(s) 144, 1373, 1376, 1802
BlsI GCNGC 4 cut(s) 145, 1374, 1377, 1803
Bme1390I CCNGG 3 cut(s) 109, 1349, 2484
Bme18I GGWCC 2 cut(s) 2405, 2599
BmeRI GACNNNNNGTC 1 cut(s) 745
BmeT110I CYCGRG 2 cut(s) 452, 1557
BmgT120I GGNCC 3 cut(s) 1000, 2405, 2599
BmiI GGNNCC 3 cut(s) 477, 2215, 2600
BmrFI CCNGG 3 cut(s) 109, 1349, 2484
BmrI ACTGGG 2 cut(s) 369, 1238
BmuI ACTGGG 2 cut(s) 369, 1238
BoxI GACNNNNGTC 1 cut(s) 1536
BpiI GAAGAC 3 cut(s) 1378, 1448, 2154
BplI GAGNNNNNCTC 2 cut(s) 635, 667
Bpu14I TTCGAA 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 2519
BsaAI YACGTR 1 cut(s) 1013
BsaI GGTCTC 1 cut(s) 298
BsaJI CCNNGG 2 cut(s) 107, 1347
BsaWI WCCGGW 3 cut(s) 686, 2470, 2551
BsaXI ACNNNNNCTCC 2 cut(s) 2352, 2382
Bsc4I CCNNNNNNNGG 9 cut(s) 26, 113, 342, 1265, 1543, 1678, 1931, 2203, 2357
Bse118I RCCGGY 1 cut(s) 2551
Bse1I ACTGG 8 cut(s) 28, 336, 375, 1233, 1330, 1425, 1824, 2570
Bse3DI GCAATG 2 cut(s) 472, 2487
BseAI TCCGGA 2 cut(s) 686, 2470
BseBI CCWGG 3 cut(s) 109, 1349, 2484
BseDI CCNNGG 2 cut(s) 107, 1347
BseLI CCNNNNNNNGG 9 cut(s) 26, 113, 342, 1265, 1543, 1678, 1931, 2203, 2357
BseMI GCAATG 2 cut(s) 472, 2487
BseMII CTCAG 9 cut(s) 141, 150, 309, 327, 725, 734, 1038, 1572, 1941
BseNI ACTGG 8 cut(s) 28, 336, 375, 1233, 1330, 1425, 1824, 2570
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 4 cut(s) 130, 1384, 1387, 1788
BseYI CCCAGC 1 cut(s) 49
BsgI GTGCAG 3 cut(s) 120, 175, 578
Bsh1236I CGCG 2 cut(s) 1391, 2438
Bsh1285I CGRYCG 1 cut(s) 2556
BshFI GGCC 3 cut(s) 1002, 1333, 2671
BshTI ACCGGT 1 cut(s) 2551
BsiEI CGRYCG 1 cut(s) 2556
BsiHKAI GWGCWC 3 cut(s) 453, 1896, 2535
BsiHKCI CYCGRG 2 cut(s) 452, 1557
BsiSI CCGG 3 cut(s) 687, 2471, 2552
BslFI GGGAC 5 cut(s) 647, 1411, 2052, 2111, 2612
BslI CCNNNNNNNGG 9 cut(s) 26, 113, 342, 1265, 1543, 1678, 1931, 2203, 2357
BsmAI GTCTC 6 cut(s) 149, 246, 298, 536, 612, 2169
BsmFI GGGAC 5 cut(s) 647, 1411, 2052, 2111, 2612
BsmI GAATGC 2 cut(s) 2430, 2492
BsnI GGCC 3 cut(s) 1002, 1333, 2671
Bso31I GGTCTC 1 cut(s) 298
BsoBI CYCGRG 2 cut(s) 452, 1557
Bsp119I TTCGAA 1 cut(s) 383
Bsp1286I GDGCHC 3 cut(s) 453, 1896, 2535
Bsp13I TCCGGA 2 cut(s) 686, 2470
BspACI CCGC 4 cut(s) 4, 92, 1391, 2408
BspANI GGCC 3 cut(s) 1002, 1333, 2671
BspCNI CTCAG 9 cut(s) 140, 151, 308, 326, 724, 733, 1037, 1573, 1942
BspEI TCCGGA 2 cut(s) 686, 2470
BspFNI CGCG 2 cut(s) 1391, 2438
BspHI TCATGA 4 cut(s) 237, 1693, 2083, 2107
BspLI GGNNCC 3 cut(s) 477, 2215, 2600
BspMAI CTGCAG 1 cut(s) 493
BspPI GGATC 5 cut(s) 813, 1285, 1355, 2021, 2495
BspQI GCTCTTC 3 cut(s) 582, 978, 1926
BspT104I TTCGAA 1 cut(s) 383
BspTI CTTAAG 2 cut(s) 848, 1796
BspTNI GGTCTC 1 cut(s) 298
BsrDI GCAATG 2 cut(s) 472, 2487
BsrFI RCCGGY 1 cut(s) 2551
BsrI ACTGG 8 cut(s) 28, 336, 375, 1233, 1330, 1425, 1824, 2570
BssAI RCCGGY 1 cut(s) 2551
BssECI CCNNGG 2 cut(s) 107, 1347
BssNAI GTATAC 1 cut(s) 1702
Bst1107I GTATAC 1 cut(s) 1702
Bst2UI CCWGG 3 cut(s) 109, 1349, 2484
Bst4CI ACNGT 7 cut(s) 787, 1135, 1473, 1483, 1613, 2026, 2651
Bst6I CTCTTC 3 cut(s) 582, 978, 1926
BstAFI CTTAAG 2 cut(s) 848, 1796
BstBAI YACGTR 1 cut(s) 1013
BstBI TTCGAA 1 cut(s) 383
BstC8I GCNNGC 6 cut(s) 194, 480, 489, 1673, 1917, 2249
BstENI CCTNNNNNAGG 1 cut(s) 1929
BstFNI CGCG 2 cut(s) 1391, 2438
BstH2I RGCGCY 2 cut(s) 569, 1322
BstHHI GCGC 2 cut(s) 568, 1321
BstMAI GTCTC 6 cut(s) 149, 246, 298, 536, 612, 2169
BstMCI CGRYCG 1 cut(s) 2556
BstMWI GCNNNNNNNGC 5 cut(s) 98, 484, 488, 572, 1801
BstNI CCWGG 3 cut(s) 109, 1349, 2484
BstNSI RCATGY 3 cut(s) 607, 640, 1919
BstPAI GACNNNNGTC 1 cut(s) 1536
BstSCI CCNGG 3 cut(s) 107, 1347, 2482
BstSFI CTRYAG 3 cut(s) 489, 1167, 2025
BstUI CGCG 2 cut(s) 1391, 2438
BstV1I GCAGC 4 cut(s) 130, 1384, 1387, 1788
BstV2I GAAGAC 3 cut(s) 1378, 1448, 2154
BstX2I RGATCY 2 cut(s) 805, 2013
BstYI RGATCY 2 cut(s) 805, 2013
BstZ17I GTATAC 1 cut(s) 1702
BsuRI GGCC 3 cut(s) 1002, 1333, 2671
BtsI GCAGTG 3 cut(s) 357, 590, 1950
BtsIMutI CAGTG 8 cut(s) 357, 590, 783, 1226, 1418, 1618, 1950, 2008
Cac8I GCNNGC 6 cut(s) 194, 480, 489, 1673, 1917, 2249
CaiI CAGNNNCTG 1 cut(s) 1949
CciI TCATGA 4 cut(s) 237, 1693, 2083, 2107
CfoI GCGC 2 cut(s) 568, 1321
Cfr10I RCCGGY 1 cut(s) 2551
Cfr13I GGNCC 3 cut(s) 1000, 2405, 2599
Csp6I GTAC 4 cut(s) 54, 845, 1484, 1813
CspAI ACCGGT 1 cut(s) 2551
CspCI CAANNNNNGTGG 2 cut(s) 2187, 2222
CviQI GTAC 4 cut(s) 54, 845, 1484, 1813
DraI TTTAAA 2 cut(s) 423, 2664
DrdI GACNNNNNNGTC 1 cut(s) 2156
DriI GACNNNNNGTC 1 cut(s) 745
DseDI GACNNNNNNGTC 1 cut(s) 2156
EaeI YGGCCR 1 cut(s) 2669
Eam1104I CTCTTC 3 cut(s) 582, 978, 1926
Eam1105I GACNNNNNGTC 1 cut(s) 745
EarI CTCTTC 3 cut(s) 582, 978, 1926
Ecl136II GAGCTC 2 cut(s) 451, 2533
Eco24I GRGCYC 2 cut(s) 453, 2535
Eco31I GGTCTC 1 cut(s) 298
Eco32I GATATC 1 cut(s) 832
Eco47I GGWCC 2 cut(s) 2405, 2599
Eco47III AGCGCT 2 cut(s) 567, 1320
Eco53kI GAGCTC 2 cut(s) 451, 2533
Eco57I CTGAAG 2 cut(s) 1236, 2303
Eco88I CYCGRG 2 cut(s) 452, 1557
EcoICRI GAGCTC 2 cut(s) 451, 2533
EcoNI CCTNNNNNAGG 1 cut(s) 1929
EcoO109I RGGNCCY 1 cut(s) 1000
EcoRII CCWGG 3 cut(s) 107, 1347, 2482
EcoRV GATATC 1 cut(s) 832
EcoT22I ATGCAT 1 cut(s) 642
EcoT38I GRGCYC 2 cut(s) 453, 2535
FalI AAGNNNNNCTT 4 cut(s) 736, 768, 1619, 1651
FaqI GGGAC 5 cut(s) 647, 1411, 2052, 2111, 2612
FbaI TGATCA 2 cut(s) 1972, 2620
FblI GTMKAC 2 cut(s) 749, 1701
Fnu4HI GCNGC 4 cut(s) 144, 1373, 1376, 1802
FriOI GRGCYC 2 cut(s) 453, 2535
Fsp4HI GCNGC 4 cut(s) 144, 1373, 1376, 1802
FspBI CTAG 7 cut(s) 168, 881, 974, 1497, 2094, 2222, 2676
GlaI GCGC 2 cut(s) 567, 1320
GluI GCNGC 4 cut(s) 144, 1373, 1376, 1802
GsaI CCCAGC 1 cut(s) 53
HaeII RGCGCY 2 cut(s) 569, 1322
HaeIII GGCC 3 cut(s) 1002, 1333, 2671
HapII CCGG 3 cut(s) 687, 2471, 2552
HhaI GCGC 2 cut(s) 568, 1321
Hin6I GCGC 2 cut(s) 566, 1319
HinP1I GCGC 2 cut(s) 566, 1319
HincII GTYRAC 1 cut(s) 2442
HindII GTYRAC 1 cut(s) 2442
HpaI GTTAAC 1 cut(s) 2442
HpaII CCGG 3 cut(s) 687, 2471, 2552
HphI GGTGA 2 cut(s) 416, 470
Hpy166II GTNNAC 6 cut(s) 407, 750, 1178, 1702, 2022, 2442
Hpy8I GTNNAC 6 cut(s) 407, 750, 1178, 1702, 2022, 2442
Hpy99I CGWCG 1 cut(s) 2438
HpyAV CCTTC 7 cut(s) 36, 276, 553, 880, 1708, 1925, 2278
HpyCH4III ACNGT 7 cut(s) 787, 1135, 1473, 1483, 1613, 2026, 2651
HpyCH4IV ACGT 3 cut(s) 941, 1012, 1106
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 484, 488, 572, 1801
HpySE526I ACGT 3 cut(s) 941, 1012, 1106
HspAI GCGC 2 cut(s) 566, 1319
Kpn2I TCCGGA 2 cut(s) 686, 2470
Ksp22I TGATCA 2 cut(s) 1972, 2620
KspAI GTTAAC 1 cut(s) 2442
LguI GCTCTTC 3 cut(s) 582, 978, 1926
LmnI GCTCC 4 cut(s) 448, 475, 1252, 2627
Lsp1109I GCAGC 4 cut(s) 130, 1384, 1387, 1788
MaeI CTAG 7 cut(s) 168, 881, 974, 1497, 2094, 2222, 2676
MaeII ACGT 3 cut(s) 941, 1012, 1106
MaeIII GTNAC 6 cut(s) 781, 976, 1308, 1561, 1945, 1982
MflI RGATCY 2 cut(s) 805, 2013
MhlI GDGCHC 3 cut(s) 453, 1896, 2535
MlsI TGGCCA 1 cut(s) 2671
MluNI TGGCCA 1 cut(s) 2671
MlyI GAGTC 3 cut(s) 212, 1016, 2093
MmeI TCCRAC 1 cut(s) 925
Mox20I TGGCCA 1 cut(s) 2671
Mph1103I ATGCAT 1 cut(s) 642
MroI TCCGGA 2 cut(s) 686, 2470
MscI TGGCCA 1 cut(s) 2671
MslI CAYNNNNRTG 3 cut(s) 177, 1119, 1200
Msp20I TGGCCA 1 cut(s) 2671
MspCI CTTAAG 2 cut(s) 848, 1796
MspI CCGG 3 cut(s) 687, 2471, 2552
MspR9I CCNGG 3 cut(s) 109, 1349, 2484
Mva1269I GAATGC 2 cut(s) 2430, 2492
MvaI CCWGG 3 cut(s) 109, 1349, 2484
MvnI CGCG 2 cut(s) 1391, 2438
MwoI GCNNNNNNNGC 5 cut(s) 98, 484, 488, 572, 1801
NlaIV GGNNCC 3 cut(s) 477, 2215, 2600
NmuCI GTSAC 3 cut(s) 781, 1561, 1945
NsiI ATGCAT 1 cut(s) 642
NspI RCATGY 3 cut(s) 607, 640, 1919
NspV TTCGAA 1 cut(s) 383
PaeI GCATGC 1 cut(s) 1919
PaeR7I CTCGAG 2 cut(s) 452, 1557
PagI TCATGA 4 cut(s) 237, 1693, 2083, 2107
PciI ACATGT 1 cut(s) 603
PciSI GCTCTTC 3 cut(s) 582, 978, 1926
PctI GAATGC 2 cut(s) 2430, 2492
PfeI GAWTC 7 cut(s) 332, 965, 1223, 1547, 1742, 1864, 2272
PflMI CCANNNNNTGG 2 cut(s) 342, 1678
PinAI ACCGGT 1 cut(s) 2551
PkrI GCNGC 4 cut(s) 145, 1374, 1377, 1803
PleI GAGTC 3 cut(s) 212, 1016, 2093
PpsI GAGTC 3 cut(s) 212, 1016, 2093
Ppu21I YACGTR 1 cut(s) 1013
PscI ACATGT 1 cut(s) 603
PshAI GACNNNNGTC 1 cut(s) 1536
PsiI TTATAA 1 cut(s) 2414
Psp124BI GAGCTC 2 cut(s) 453, 2535
Psp6I CCWGG 3 cut(s) 107, 1347, 2482
PspFI CCCAGC 1 cut(s) 49
PspGI CCWGG 3 cut(s) 107, 1347, 2482
PspN4I GGNNCC 3 cut(s) 477, 2215, 2600
PspPI GGNCC 3 cut(s) 1000, 2405, 2599
PspXI VCTCGAGB 2 cut(s) 452, 1557
PstI CTGCAG 1 cut(s) 493
PstNI CAGNNNCTG 1 cut(s) 1949
PsuI RGATCY 2 cut(s) 805, 2013
RsaI GTAC 4 cut(s) 55, 846, 1485, 1814
RsaNI GTAC 4 cut(s) 54, 845, 1484, 1813
RseI CAYNNNNRTG 3 cut(s) 177, 1119, 1200
SacI GAGCTC 2 cut(s) 453, 2535
SapI GCTCTTC 3 cut(s) 582, 978, 1926
SatI GCNGC 4 cut(s) 144, 1373, 1376, 1802
Sau96I GGNCC 3 cut(s) 1000, 2405, 2599
SchI GAGTC 3 cut(s) 212, 1016, 2093
ScrFI CCNGG 3 cut(s) 109, 1349, 2484
SduI GDGCHC 3 cut(s) 453, 1896, 2535
SfcI CTRYAG 3 cut(s) 489, 1167, 2025
Sfr274I CTCGAG 2 cut(s) 452, 1557
SfuI TTCGAA 1 cut(s) 383
SinI GGWCC 2 cut(s) 2405, 2599
SlaI CTCGAG 2 cut(s) 452, 1557
SmiI ATTTAAAT 1 cut(s) 2664
SmiMI CAYNNNNRTG 3 cut(s) 177, 1119, 1200
SmlI CTYRAG 5 cut(s) 452, 848, 1557, 1796, 2534
SmoI CTYRAG 5 cut(s) 452, 848, 1557, 1796, 2534
SpeI ACTAGT 1 cut(s) 880
SphI GCATGC 1 cut(s) 1919
SsiI CCGC 4 cut(s) 4, 92, 1391, 2408
SspMI CTAG 7 cut(s) 168, 881, 974, 1497, 2094, 2222, 2676
SstI GAGCTC 2 cut(s) 453, 2535
StyD4I CCNGG 3 cut(s) 107, 1347, 2482
SwaI ATTTAAAT 1 cut(s) 2664
TaaI ACNGT 7 cut(s) 787, 1135, 1473, 1483, 1613, 2026, 2651
TaiI ACGT 3 cut(s) 944, 1015, 1109
TaqI TCGA 6 cut(s) 383, 453, 580, 1020, 1558, 2585
TatI WGTACW 2 cut(s) 1483, 1812
TfiI GAWTC 7 cut(s) 332, 965, 1223, 1547, 1742, 1864, 2272
TscAI CASTG 8 cut(s) 357, 597, 790, 1233, 1425, 1618, 1950, 2008
TseFI GTSAC 3 cut(s) 781, 1561, 1945
TseI GCWGC 4 cut(s) 143, 1372, 1375, 1801
Tsp45I GTSAC 3 cut(s) 781, 1561, 1945
TspGWI ACGGA 2 cut(s) 71, 1526
TspRI CASTG 8 cut(s) 357, 597, 790, 1233, 1425, 1618, 1950, 2008
Van91I CCANNNNNTGG 2 cut(s) 342, 1678
Vha464I CTTAAG 2 cut(s) 848, 1796
VpaK11BI GGWCC 2 cut(s) 2405, 2599
XagI CCTNNNNNAGG 1 cut(s) 1929
XapI RAATTY 4 cut(s) 281, 647, 2450, 2635
XceI RCATGY 3 cut(s) 607, 640, 1919
XcmI CCANNNNNNNNNTGG 2 cut(s) 46, 343
XhoI CTCGAG 2 cut(s) 452, 1557
XmiI GTMKAC 2 cut(s) 749, 1701
XspI CTAG 7 cut(s) 168, 881, 974, 1497, 2094, 2222, 2676
Zsp2I ATGCAT 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.