Rroxscaffold_5G00386920

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009991
Physical Location & Seq
Reverse (-)
65646772 .. 65655906
9135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386920.1

Sequence Viewer

Length: 3312 bp
ATGCCGACGACAAACCTGGTGCTCCTGGCGTGCTTGGTGTCGCTGGTGGCAGCAATCGCCGCCATTGATTCAAAATCAGACAGCTCCGGCGAGTGGCACATCCTCACGAAGCAGAACTTCTCTTCTCAGATCCATCTCCATCCTCACATCCTCCTCCTCGTCACTCTTCCATGGTCCGGTGAGTGTCGATCACTCATGAGAGACGTGGCGAAGTTGGTCACTGATAGACCAGAAGAGTTCAGCTCGCTGAAGCTGATGGTGTTGCACAGAAATACAGAGAAGATGGTGGCAACTGCGATCGGTGCTGCTTCAGAATGGGAGGAAATTACGGTTTTGTACTATCACCATTCTGTTTCGTACAAATATGGAGGCAGGCTTCGAGCGCAGAACATTTTGAGTTCAATTCGTCCCTATGTGTCGGTTTTAGGTGAAGAACTTCCATTCAAATCGCTGAAGAGTCCGGAAGAGTTGAAAGCTTTCGTTGATTCAACTGACAAATCTTTGGTTGTGTTTGAGTTTTGTGAATGGACTCCTAAATTGGTGGCCAAGAGGAAGATGAATGGGACTGATCACACTGGTTTTGAGTTTGGAGTACAATTCTGCTGTTTGCATGTTGCATATGTCCATTGTCCATCTTTTTCATTTCTCTTTATTACCTTCAACGATGGAGGTATGGAAACTGCAAAGATGAAGTGTAATGTTGATAATGAGCTTGGTGCAGTTCCTTGGGTTGGGGACTTCAGCTCAGTAAATGACAGTGCTGCTTCTGAAGAGACTGAGAACAGTAGGCCTGATGGTGCATCATTCTGCACACTCGAAGAATATCAGTTGTTTGACTCTTTCTTCTCAAAGTTCATGACTATTGCAAGAGAGTTCGTTCTGCCTCCTGAAAGGCATAAATTTGGTTTGGTCTCAGAAAGATCTATGCTCTCAGCTCTTGATGTTAGAGATTCCAGTTCGTGGTTGGCAGTGCTATCCTTTGCTGGATGTCCCAGTTGTTCGAAGATTATTAAAAAAGAGGATGACCTTAATAATGCTTTAAAGATGGATAGTTTAGTTAAGGAGCTAGAGGGTCATGGCAACACTTTGGAGCCTGCTTTGCCTGCAAACCAGCCATCAGTTCTCTTATTTGTGGATAGATCATCTGACTTGTTAGAGACCAGAATCAATGGCAAGGAAGCTCTTGATGCTTTTCGGAAGCTGGCACTGCACTATCACATGTCTGACCAGATTGGCAGTCAAAGTAAAGACAAGCATGAGAAATCCTCATCCCAGCATTATCAAGCATTAAAGACCACATCTGGACATCCTAAATTAAAATTATCTCAGACAGTGCAGATTAGCAAACTAGAAGACAAGATGTCTACTTTCACAATTATAAATGAGGGCAAACAATTCACAGTGGAGAAGTTGGCTTTGGATCTAAAGGGTAATTCCTTGCAGGATATCTTGGAAATTGTACTTAAGCAAAAGAAGAAAGCTAAGTTAAGCTCACTGGTGAAGGATTTGGGTTTCCAACTTTTGTCAGATGATATGGACATCAAACCAGAGAGTACATTGCCATTACCGATGGAATCTGAATCTGATACAGTTACAGAAGAGCCATATAAGGAGGGCCTTGCTACAAGTAGTACTGACTCAGACAGAGATCAACTGCCAGATGCAACAAGTATATCTACCGAACAGCATCCAGAAACTTCTGAAGTCATGGATACTGAAACGTCATCTCAGAACAATGAAGAAAAAACAGATTATGTTGATACAAGCAATCAGATCTTATCTGTCGCTAGTGAGCAACAGCTTGCAGATCATAAACATGAAGATTTAGATTTAGATACAGAAGAAGATAGTAATCCACAAGGCGAAAAGTCAGCAGAGCAGCAGCTTCACTTTCAAGGCTTTAAGGGTTCATTTTTCTTTTCTGATGGTAATTGTCGGTTGCTTCGAGCGCTGACTGGCAGTTCAAAATTTCCAGCCCTGGTAATAGTTGATCCCAATATGCAGCAGCATTATGTCTTCCCAGAGGAGACTAATGTCAATTATTCTTCACTGGTTGATTTTGTTTCCGCCTTTCTTAATGGAAGTCTTCTTCCATATCAACAGTCTGAAACTGTACTTGAAAGCTCTAGGAAAGCCACTCAGCCACCATTTGTTAATCTGGACTTCCATCAGGTGGATTCTATCCCTCGAGTGACAACTCACACTTTCTCTGAGCTGGTTGTTGGGTTCAATCAATCTGACAGTGATGCTTGGAATAAGGATGTCTTGGTTCTTTTTACCAATAGATGGTGTGGTTTTTGCCAGCGAATGGAGTTAGTTGTTCATGAAGTATACCGAGCTATGAAGGATTATGCTAAGATGCTGAAAAGGGAATCAAAGAATGACAAATCAACGTTTCATGATAATATGACGGGCGACGTGAAAACTGAAATGCTGGAGCTGCCATTAATGTACTTGCTGGATTGCACATTGAATGATTGCAGTTTGATACTGAAATCGATGAATCAGAGGGAGGTTTATCCTACTCTGGTGCTCTTTCCAGCAGAAAAGAAGCATGCTCTTCCTTATGAAGGAGATATGGCAGTCACTGAGATTTTCAATTTCATGGCTGATCATGGAAGTAACTCTCATTATCTTACCAGTGAAAAAGGAATGTTGTGGACTTTAGCTGAAAAGAGAGGAAGAAATCAAGATTTCTTTGTGGTTCAGTCATCTGACGTTCATGAGCAGTCTAGGGACACGCTTCATGAAGTCCTATTAACAAATGTTCATAAACCAGTAATTGAAGACAAGCAGATCAAGTCTCACATACCCCATGAAGCACCCCCGAATGTGGTGGTTGGTTCTGTCCTAGTTGCTACTGATAAACTTCATAGTGTACACCCCTTTGATAAATCAGAGATTCTCATTGTGAAGGCAGATCAAGTTACTGGATTTCAAGGTTTAATTATCAACAAGCATATCAGATGGGATGCTCTGCCTGAATTGGGAGAAGGAGTTAAGATGTTAGTAGAAGCCCCTTTGTCCTTTGGAGGACCGCTTATAAAGGGTGGAATGCCTCTTGTCGCGTTAACTCAAAAATTTGTCAAGGATGAATATCCGGAAATCTTACCAGGCATTGCATTTCTTGATCCATCAGCAACAATTCAAAAAATCAAAGAGCTCAACTTAGGAAATCAATCTGTCACTGACTACTGGTTTTTCTTTGGGTATTCAAGTTGGGTTTGGGACCAGCTCTTTGATGAGATTGATAAGGGAGCTTGGAATTTAAGTGACGATGGTATGAGACATTTAGATTGGCCATCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179 Thioredoxin

Protein Analysis

1103

Amino Acids

124.05

Weight (kDa)

5.16

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 963 - 1092 6.1e-10 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1379, 3044
AccB7I CCANNNNNTGG 4 cut(s) 960, 2173, 2286, 2308
AccI GTMKAC 2 cut(s) 1364, 2331
AccII CGCG 1 cut(s) 3068
AccIII TCCGGA 2 cut(s) 460, 3100
AciI CCGC 3 cut(s) 60, 2067, 3038
AclI AACGTT 1 cut(s) 2393
AclWI GGATC 4 cut(s) 124, 1428, 1985, 3125
AcoI YGGCCR 2 cut(s) 543, 3299
AcsI RAATTY 4 cut(s) 899, 1967, 3080, 3265
AcuI CTGAAG 6 cut(s) 269, 294, 473, 724, 789, 1722
AfaI GTAC 9 cut(s) 338, 359, 594, 1461, 1555, 1633, 2115, 2453, 2880
AfeI AGCGCT 1 cut(s) 1950
AflII CTTAAG 1 cut(s) 1463
AflIII ACRYGT 1 cut(s) 1218
AhdI GACNNNNNGTC 1 cut(s) 1360
AjiI CACGTC 2 cut(s) 205, 2419
AjnI CCWGG 4 cut(s) 15, 24, 1977, 3112
AjuI GAANNNNNNNTTGG 2 cut(s) 1400, 1432
Alw21I GWGCWC 3 cut(s) 24, 2535, 3165
Alw26I GTCTC 7 cut(s) 195, 767, 916, 1151, 2021, 2808, 3280
AlwI GGATC 4 cut(s) 124, 1428, 1985, 3125
AlwNI CAGNNNCTG 1 cut(s) 2588
Ama87I CYCGRG 1 cut(s) 2187
Aor13HI TCCGGA 2 cut(s) 460, 3100
Aor51HI AGCGCT 1 cut(s) 1950
AoxI GGCC 4 cut(s) 543, 788, 1615, 3299
ApeKI GCWGC 8 cut(s) 50, 305, 761, 1879, 1882, 2002, 2005, 2440
ApoI RAATTY 4 cut(s) 899, 1967, 3080, 3265
ArsI GACNNNNNNTTYG 2 cut(s) 3221, 3253
AseI ATTAAT 1 cut(s) 2447
Asp700I GAANNNNTTC 2 cut(s) 435, 476
AspLEI GCGC 2 cut(s) 385, 1951
AspS9I GGNCC 4 cut(s) 174, 1615, 3035, 3229
AsuHPI GGTGA 4 cut(s) 191, 335, 440, 1510
AsuII TTCGAA 1 cut(s) 1001
AvaI CYCGRG 1 cut(s) 2187
AvaII GGWCC 3 cut(s) 174, 3035, 3229
BalI TGGCCA 2 cut(s) 545, 3301
BanII GRGCYC 1 cut(s) 3165
BbsI GAAGAC 4 cut(s) 1359, 2008, 2078, 2793
Bbv12I GWGCWC 3 cut(s) 24, 2535, 3165
BbvI GCAGC 8 cut(s) 62, 292, 748, 1891, 1894, 2014, 2017, 2427
BciT130I CCWGG 4 cut(s) 17, 26, 1979, 3114
BciVI GTATCC 1 cut(s) 1705
BclI TGATCA 2 cut(s) 568, 2611
BcoDI GTCTC 7 cut(s) 195, 767, 916, 1151, 2021, 2808, 3280
BfaI CTAG 6 cut(s) 1067, 1349, 1788, 2127, 2733, 2852
BfoI RGCGCY 1 cut(s) 1952
BfrI CTTAAG 1 cut(s) 1463
BfuI GTATCC 1 cut(s) 1705
BglII AGATCT 2 cut(s) 920, 1773
BisI GCNGC 9 cut(s) 51, 60, 306, 762, 1880, 1883, 2003, 2006, 2441
BlsI GCNGC 9 cut(s) 52, 61, 307, 763, 1881, 1884, 2004, 2007, 2442
BmcAI AGTACT 1 cut(s) 1633
Bme1390I CCNGG 4 cut(s) 17, 26, 1979, 3114
Bme18I GGWCC 3 cut(s) 174, 3035, 3229
BmeRI GACNNNNNGTC 1 cut(s) 1360
BmeT110I CYCGRG 1 cut(s) 2187
BmgBI CACGTC 2 cut(s) 205, 2419
BmgT120I GGNCC 4 cut(s) 174, 1615, 3035, 3229
BmiI GGNNCC 2 cut(s) 1092, 3230
BmrFI CCNGG 4 cut(s) 17, 26, 1979, 3114
BmrI ACTGGG 1 cut(s) 987
BmsI GCATC 7 cut(s) 809, 1177, 1651, 1696, 2236, 2349, 2962
BmuI ACTGGG 1 cut(s) 987
BoxI GACNNNNGTC 1 cut(s) 2033
BpiI GAAGAC 4 cut(s) 1359, 2008, 2078, 2793
BplI GAGNNNNNCTC 4 cut(s) 227, 259, 1250, 1282
BpmI CTGGAG 1 cut(s) 2456
Bpu14I TTCGAA 1 cut(s) 1001
Bsa29I ATCGAT 1 cut(s) 2498
BsaBI GATNNNNATC 2 cut(s) 1851, 3096
BsaI GGTCTC 2 cut(s) 916, 1151
BsaJI CCNNGG 3 cut(s) 170, 725, 1977
BsaWI WCCGGW 3 cut(s) 176, 460, 3100
BsaXI ACNNNNNCTCC 2 cut(s) 2982, 3012
Bse3DI GCAATG 2 cut(s) 1556, 3117
Bse8I GATNNNNATC 2 cut(s) 1851, 3096
BseAI TCCGGA 2 cut(s) 460, 3100
BseBI CCWGG 4 cut(s) 17, 26, 1979, 3114
BseCI ATCGAT 1 cut(s) 2498
BseDI CCNNGG 3 cut(s) 170, 725, 1977
BseJI GATNNNNATC 2 cut(s) 1851, 3096
BseMI GCAATG 2 cut(s) 1556, 3117
BseRI GAGGAG 3 cut(s) 143, 146, 2039
BseXI GCAGC 8 cut(s) 62, 292, 748, 1891, 1894, 2014, 2017, 2427
BseYI CCCAGC 1 cut(s) 1272
BsgI GTGCAG 4 cut(s) 738, 793, 1193, 1355
Bsh1236I CGCG 1 cut(s) 3068
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 4 cut(s) 545, 790, 1617, 3301
BshVI ATCGAT 1 cut(s) 2498
BsiEI CGRYCG 1 cut(s) 300
BsiHKAI GWGCWC 3 cut(s) 24, 2535, 3165
BsiHKCI CYCGRG 1 cut(s) 2187
BsiSI CCGG 4 cut(s) 87, 177, 461, 3101
BslFI GGGAC 6 cut(s) 393, 577, 749, 975, 2750, 3242
BsmAI GTCTC 7 cut(s) 195, 767, 916, 1151, 2021, 2808, 3280
BsmBI CGTCTC 1 cut(s) 195
BsmFI GGGAC 6 cut(s) 393, 577, 749, 975, 2750, 3242
BsmI GAATGC 1 cut(s) 3060
BsnI GGCC 4 cut(s) 545, 790, 1617, 3301
Bso31I GGTCTC 2 cut(s) 916, 1151
BsoBI CYCGRG 1 cut(s) 2187
Bsp119I TTCGAA 1 cut(s) 1001
Bsp1286I GDGCHC 3 cut(s) 24, 2535, 3165
Bsp13I TCCGGA 2 cut(s) 460, 3100
Bsp1407I TGTACA 1 cut(s) 2878
Bsp19I CCATGG 1 cut(s) 170
BspACI CCGC 3 cut(s) 60, 2067, 3038
BspANI GGCC 4 cut(s) 545, 790, 1617, 3301
BspDI ATCGAT 1 cut(s) 2498
BspEI TCCGGA 2 cut(s) 460, 3100
BspFNI CGCG 1 cut(s) 3068
BspHI TCATGA 6 cut(s) 195, 855, 2323, 2398, 2722, 2746
BspLI GGNNCC 2 cut(s) 1092, 3230
BspPI GGATC 4 cut(s) 124, 1428, 1985, 3125
BspQI GCTCTTC 2 cut(s) 1593, 2565
BspT104I TTCGAA 1 cut(s) 1001
BspTI CTTAAG 1 cut(s) 1463
BspTNI GGTCTC 2 cut(s) 916, 1151
BsrDI GCAATG 2 cut(s) 1556, 3117
BsrGI TGTACA 1 cut(s) 2878
BssECI CCNNGG 3 cut(s) 170, 725, 1977
BssNAI GTATAC 1 cut(s) 2332
BssT1I CCWWGG 2 cut(s) 170, 725
Bst1107I GTATAC 1 cut(s) 2332
Bst2UI CCWGG 4 cut(s) 17, 26, 1979, 3114
Bst4CI ACNGT 9 cut(s) 331, 758, 785, 1333, 1402, 1591, 2103, 2113, 2243
Bst6I CTCTTC 8 cut(s) 127, 171, 228, 449, 459, 765, 1593, 2565
BstAFI CTTAAG 1 cut(s) 1463
BstAUI TGTACA 1 cut(s) 2878
BstBI TTCGAA 1 cut(s) 1001
BstC8I GCNNGC 9 cut(s) 31, 245, 374, 1095, 1104, 1203, 1803, 2303, 2556
BstDSI CCRYGG 1 cut(s) 170
BstENI CCTNNNNNAGG 1 cut(s) 2568
BstFNI CGCG 1 cut(s) 3068
BstH2I RGCGCY 1 cut(s) 1952
BstHHI GCGC 2 cut(s) 385, 1951
BstMAI GTCTC 7 cut(s) 195, 767, 916, 1151, 2021, 2808, 3280
BstMCI CGRYCG 1 cut(s) 300
BstNI CCWGG 4 cut(s) 17, 26, 1979, 3114
BstNSI RCATGY 3 cut(s) 614, 1222, 2558
BstPAI GACNNNNGTC 1 cut(s) 2033
BstSCI CCNGG 4 cut(s) 15, 24, 1977, 3112
BstUI CGCG 1 cut(s) 3068
BstV1I GCAGC 8 cut(s) 62, 292, 748, 1891, 1894, 2014, 2017, 2427
BstV2I GAAGAC 4 cut(s) 1359, 2008, 2078, 2793
BstX2I RGATCY 4 cut(s) 129, 920, 1420, 1773
BstYI RGATCY 4 cut(s) 129, 920, 1420, 1773
BstZ17I GTATAC 1 cut(s) 2332
Bsu15I ATCGAT 1 cut(s) 2498
BsuI GTATCC 1 cut(s) 1705
BsuRI GGCC 4 cut(s) 545, 790, 1617, 3301
BsuTUI ATCGAT 1 cut(s) 2498
BtgI CCRYGG 1 cut(s) 170
BtrI CACGTC 2 cut(s) 205, 2419
BtsI GCAGTG 2 cut(s) 975, 1205
Cac8I GCNNGC 9 cut(s) 31, 245, 374, 1095, 1104, 1203, 1803, 2303, 2556
CaiI CAGNNNCTG 1 cut(s) 2588
CciI TCATGA 6 cut(s) 195, 855, 2323, 2398, 2722, 2746
CfoI GCGC 2 cut(s) 385, 1951
Cfr13I GGNCC 4 cut(s) 174, 1615, 3035, 3229
ClaI ATCGAT 1 cut(s) 2498
CsiI ACCWGGT 1 cut(s) 15
Csp6I GTAC 9 cut(s) 337, 358, 593, 1460, 1554, 1632, 2114, 2452, 2879
CviQI GTAC 9 cut(s) 337, 358, 593, 1460, 1554, 1632, 2114, 2452, 2879
DraI TTTAAA 1 cut(s) 1041
DriI GACNNNNNGTC 1 cut(s) 1360
EaeI YGGCCR 2 cut(s) 543, 3299
Eam1104I CTCTTC 8 cut(s) 127, 171, 228, 449, 459, 765, 1593, 2565
Eam1105I GACNNNNNGTC 1 cut(s) 1360
EarI CTCTTC 8 cut(s) 127, 171, 228, 449, 459, 765, 1593, 2565
EciI GGCGGA 1 cut(s) 2056
Ecl136II GAGCTC 1 cut(s) 3163
Eco130I CCWWGG 2 cut(s) 170, 725
Eco147I AGGCCT 1 cut(s) 790
Eco24I GRGCYC 1 cut(s) 3165
Eco31I GGTCTC 2 cut(s) 916, 1151
Eco32I GATATC 1 cut(s) 1447
Eco47I GGWCC 3 cut(s) 174, 3035, 3229
Eco47III AGCGCT 1 cut(s) 1950
Eco53kI GAGCTC 1 cut(s) 3163
Eco57I CTGAAG 6 cut(s) 269, 294, 473, 724, 789, 1722
Eco88I CYCGRG 1 cut(s) 2187
EcoICRI GAGCTC 1 cut(s) 3163
EcoNI CCTNNNNNAGG 1 cut(s) 2568
EcoO109I RGGNCCY 1 cut(s) 1615
EcoRII CCWGG 4 cut(s) 15, 24, 1977, 3112
EcoRV GATATC 1 cut(s) 1447
EcoT14I CCWWGG 2 cut(s) 170, 725
EcoT38I GRGCYC 1 cut(s) 3165
ErhI CCWWGG 2 cut(s) 170, 725
Esp3I CGTCTC 1 cut(s) 195
FalI AAGNNNNNCTT 4 cut(s) 101, 133, 2249, 2281
FaqI GGGAC 6 cut(s) 393, 577, 749, 975, 2750, 3242
FauNDI CATATG 1 cut(s) 619
FbaI TGATCA 2 cut(s) 568, 2611
FblI GTMKAC 2 cut(s) 1364, 2331
Fnu4HI GCNGC 9 cut(s) 51, 60, 306, 762, 1880, 1883, 2003, 2006, 2441
FriOI GRGCYC 1 cut(s) 3165
Fsp4HI GCNGC 9 cut(s) 51, 60, 306, 762, 1880, 1883, 2003, 2006, 2441
FspBI CTAG 6 cut(s) 1067, 1349, 1788, 2127, 2733, 2852
GlaI GCGC 2 cut(s) 384, 1950
GluI GCNGC 9 cut(s) 51, 60, 306, 762, 1880, 1883, 2003, 2006, 2441
GsaI CCCAGC 1 cut(s) 1276
GsuI CTGGAG 1 cut(s) 2456
HaeII RGCGCY 1 cut(s) 1952
HaeIII GGCC 4 cut(s) 545, 790, 1617, 3301
HapII CCGG 4 cut(s) 87, 177, 461, 3101
HhaI GCGC 2 cut(s) 385, 1951
Hin6I GCGC 2 cut(s) 383, 1949
HinP1I GCGC 2 cut(s) 383, 1949
HincII GTYRAC 1 cut(s) 3072
HindII GTYRAC 1 cut(s) 3072
HindIII AAGCTT 1 cut(s) 474
HpaI GTTAAC 1 cut(s) 3072
HpaII CCGG 4 cut(s) 87, 177, 461, 3101
HphI GGTGA 4 cut(s) 191, 335, 440, 1510
Hpy166II GTNNAC 6 cut(s) 1365, 2332, 2661, 2879, 2881, 3072
Hpy8I GTNNAC 6 cut(s) 1365, 2332, 2661, 2879, 2881, 3072
Hpy99I CGWCG 2 cut(s) 10, 2420
HpyAV CCTTC 6 cut(s) 667, 1495, 2338, 2564, 2908, 2987
HpyCH4III ACNGT 9 cut(s) 331, 758, 785, 1333, 1402, 1591, 2103, 2113, 2243
HpyCH4IV ACGT 5 cut(s) 204, 1721, 2393, 2418, 2718
HpySE526I ACGT 5 cut(s) 204, 1721, 2393, 2418, 2718
HspAI GCGC 2 cut(s) 383, 1949
Kpn2I TCCGGA 2 cut(s) 460, 3100
Ksp22I TGATCA 2 cut(s) 568, 2611
KspAI GTTAAC 1 cut(s) 3072
LguI GCTCTTC 2 cut(s) 1593, 2565
LmnI GCTCC 6 cut(s) 27, 89, 1063, 1090, 2437, 3257
Lsp1109I GCAGC 8 cut(s) 62, 292, 748, 1891, 1894, 2014, 2017, 2427
LweI GCATC 7 cut(s) 809, 1177, 1651, 1696, 2236, 2349, 2962
MabI ACCWGGT 1 cut(s) 15
MaeI CTAG 6 cut(s) 1067, 1349, 1788, 2127, 2733, 2852
MaeII ACGT 5 cut(s) 204, 1721, 2393, 2418, 2718
MaeIII GTNAC 9 cut(s) 160, 217, 1591, 2191, 2584, 2621, 2926, 3184, 3272
MflI RGATCY 4 cut(s) 129, 920, 1420, 1773
MhlI GDGCHC 3 cut(s) 24, 2535, 3165
MlsI TGGCCA 2 cut(s) 545, 3301
MluNI TGGCCA 2 cut(s) 545, 3301
MlyI GAGTC 4 cut(s) 466, 523, 830, 1631
MmeI TCCRAC 1 cut(s) 1540
Mox20I TGGCCA 2 cut(s) 545, 3301
MroI TCCGGA 2 cut(s) 460, 3100
MroXI GAANNNNTTC 2 cut(s) 435, 476
MscI TGGCCA 2 cut(s) 545, 3301
MslI CAYNNNNRTG 1 cut(s) 1815
Msp20I TGGCCA 2 cut(s) 545, 3301
MspCI CTTAAG 1 cut(s) 1463
MspI CCGG 4 cut(s) 87, 177, 461, 3101
MspR9I CCNGG 4 cut(s) 17, 26, 1979, 3114
Mva1269I GAATGC 1 cut(s) 3060
MvaI CCWGG 4 cut(s) 17, 26, 1979, 3114
MvnI CGCG 1 cut(s) 3068
NcoI CCATGG 1 cut(s) 170
NdeI CATATG 1 cut(s) 619
NlaIV GGNNCC 2 cut(s) 1092, 3230
NmuCI GTSAC 6 cut(s) 160, 217, 2191, 2584, 3184, 3272
NspI RCATGY 3 cut(s) 614, 1222, 2558
NspV TTCGAA 1 cut(s) 1001
PaeI GCATGC 1 cut(s) 2558
PaeR7I CTCGAG 1 cut(s) 2187
PagI TCATGA 6 cut(s) 195, 855, 2323, 2398, 2722, 2746
PceI AGGCCT 1 cut(s) 790
PciI ACATGT 1 cut(s) 1218
PciSI GCTCTTC 2 cut(s) 1593, 2565
PcsI WCGNNNNNNNCGW 1 cut(s) 1942
PctI GAATGC 1 cut(s) 3060
PdmI GAANNNNTTC 2 cut(s) 435, 476
PflMI CCANNNNNTGG 4 cut(s) 960, 2173, 2286, 2308
PkrI GCNGC 9 cut(s) 52, 61, 307, 763, 1881, 1884, 2004, 2007, 2442
Ple19I CGATCG 1 cut(s) 300
PleI GAGTC 4 cut(s) 465, 523, 830, 1631
PpsI GAGTC 4 cut(s) 465, 523, 830, 1631
PscI ACATGT 1 cut(s) 1218
PshAI GACNNNNGTC 1 cut(s) 2033
PshBI ATTAAT 1 cut(s) 2447
PsiI TTATAA 2 cut(s) 1379, 3044
Psp124BI GAGCTC 1 cut(s) 3165
Psp1406I AACGTT 1 cut(s) 2393
Psp6I CCWGG 4 cut(s) 15, 24, 1977, 3112
PspFI CCCAGC 1 cut(s) 1272
PspGI CCWGG 4 cut(s) 15, 24, 1977, 3112
PspN4I GGNNCC 2 cut(s) 1092, 3230
PspPI GGNCC 4 cut(s) 174, 1615, 3035, 3229
PspXI VCTCGAGB 1 cut(s) 2187
PstNI CAGNNNCTG 1 cut(s) 2588
PsuI RGATCY 4 cut(s) 129, 920, 1420, 1773
PvuI CGATCG 1 cut(s) 300
RsaI GTAC 9 cut(s) 338, 359, 594, 1461, 1555, 1633, 2115, 2453, 2880
RsaNI GTAC 9 cut(s) 337, 358, 593, 1460, 1554, 1632, 2114, 2452, 2879
RseI CAYNNNNRTG 1 cut(s) 1815
SacI GAGCTC 1 cut(s) 3165
SapI GCTCTTC 2 cut(s) 1593, 2565
SatI GCNGC 9 cut(s) 51, 60, 306, 762, 1880, 1883, 2003, 2006, 2441
Sau96I GGNCC 4 cut(s) 174, 1615, 3035, 3229
ScaI AGTACT 1 cut(s) 1633
SchI GAGTC 4 cut(s) 466, 523, 830, 1631
ScrFI CCNGG 4 cut(s) 17, 26, 1979, 3114
SduI GDGCHC 3 cut(s) 24, 2535, 3165
SexAI ACCWGGT 1 cut(s) 15
SfaNI GCATC 7 cut(s) 809, 1177, 1651, 1696, 2236, 2349, 2962
Sfr274I CTCGAG 1 cut(s) 2187
SfuI TTCGAA 1 cut(s) 1001
SinI GGWCC 3 cut(s) 174, 3035, 3229
SlaI CTCGAG 1 cut(s) 2187
SmiMI CAYNNNNRTG 1 cut(s) 1815
SmlI CTYRAG 2 cut(s) 1463, 2187
SmoI CTYRAG 2 cut(s) 1463, 2187
SphI GCATGC 1 cut(s) 2558
SseBI AGGCCT 1 cut(s) 790
SsiI CCGC 3 cut(s) 60, 2067, 3038
SspMI CTAG 6 cut(s) 1067, 1349, 1788, 2127, 2733, 2852
SstI GAGCTC 1 cut(s) 3165
StuI AGGCCT 1 cut(s) 790
StyD4I CCNGG 4 cut(s) 15, 24, 1977, 3112
StyI CCWWGG 2 cut(s) 170, 725
TaaI ACNGT 9 cut(s) 331, 758, 785, 1333, 1402, 1591, 2103, 2113, 2243
TaiI ACGT 5 cut(s) 207, 1724, 2396, 2421, 2721
TaqI TCGA 7 cut(s) 187, 379, 816, 1001, 1945, 2188, 2498
TatI WGTACW 8 cut(s) 336, 592, 1459, 1553, 1631, 2113, 2451, 2878
TauI GCSGC 1 cut(s) 62
TseFI GTSAC 6 cut(s) 160, 217, 2191, 2584, 3184, 3272
TseI GCWGC 8 cut(s) 50, 305, 761, 1879, 1882, 2002, 2005, 2440
Tsp45I GTSAC 6 cut(s) 160, 217, 2191, 2584, 3184, 3272
Van91I CCANNNNNTGG 4 cut(s) 960, 2173, 2286, 2308
Vha464I CTTAAG 1 cut(s) 1463
VpaK11BI GGWCC 3 cut(s) 174, 3035, 3229
VspI ATTAAT 1 cut(s) 2447
XagI CCTNNNNNAGG 1 cut(s) 2568
XapI RAATTY 4 cut(s) 899, 1967, 3080, 3265
XceI RCATGY 3 cut(s) 614, 1222, 2558
XcmI CCANNNNNNNNNTGG 1 cut(s) 961
XhoI CTCGAG 1 cut(s) 2187
XmiI GTMKAC 2 cut(s) 1364, 2331
XmnI GAANNNNTTC 2 cut(s) 435, 476
XspI CTAG 6 cut(s) 1067, 1349, 1788, 2127, 2733, 2852
ZrmI AGTACT 1 cut(s) 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.