Rh4CG460000

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009991
Physical Location & Seq
Reverse (-)
69090583 .. 69095909
5327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG460000.1

Sequence Viewer

Length: 3432 bp
ATGGCAACATGGTCCATGTATCCATCCGGCAAACCCAAACCCGACCCGCAAGTCCAACTCCATTCGGTTATCAAACCCAACCCAGTGGAATCGGCGAAGACTGAAGAGTGTACGCTGCACAGTGCTCGGAGAGCGATGCCGACGAGAAACCTGGTGCTCCTGGCGTGCTTGGTGTCGCTGGTGGCAGCAATCGCCGCCGTTGATTCAAAATCAGACAGCTCCGGCGAGTGGCACATCCTCACGAAGCAGAACTTCTCTTCTCAGATCCGTCTCCATCCTCACATCCTCCTCTTCGTCACTCTTCCATGGTCCGGTGAGTGTCGATCACTCATGAGAGACGTGGCGAAGTTGGTCGCTGATCGACCTGAAGAGTTCAGCTCGCTGAAGCTGATGGTGTTGCACAGAAATACAGAGAAGATGGTGGCAACTGCGATCGGTGCTGCTTCAGAATGGGAGGAAATTACGGTTTTGTACTATCACCATTCTGTTTCGTACAAATATGGAGGGAGGCTTCGAGCGCAGAGCATTTTGAGTTCAATTCGTCCCTATGTGTCGGTTTTAGGTGAAGAACTTCCATTCAAATCGCTGAAGAGTCCGGAAGAGTTGAAAGCTTTCGTTGATTCAACTGACAAAGCTTTGGTCGTGTTTGAGTTTTGTGAATGGACTCCTAAATTGGTGGCCAAGAGGAAGATGAATGGGACTGATCACAGTGGCTTTGGGGAATTTTTTGGGTTAAACTTGAATGCAGAGACAAATAGAACCGACTGGCGGAAGAATAACCAGAAGGGTATGGAAACTGCAAAGATGAAGTGTAATGTTGATAATGAGCTTGGTGCAGTTCCTTGGGTTGGGGACTTCAGCTCAGTAAATGACAGTGCTGCTTCTGAGGAGACTGAGAACAGTAGGCCTGATGGTGCATCGTTTTGCACACTTGAAGAATATCAGTTGTTTGACTCTTTCTTCTCAAAGTTCATGACTATTGCAAGAGAGTTCGTTCTGCCTCCTGAAAGGCATAAATTTGGTTTGGTCTCAGAAAGATCTATGCTCTCAGCTCTTGGTATTGGAGATTCCAGTTCGTGGTTGGCAGTGCTATACTTTGCTGGATGTCCCAGTTGTTCGAAGATTATTAAAAAAGAGGATGACCTTAATAATGCTTTAAAGATGGATAGTTTAGTTAAGGAGCTGGAGGGTGATGGCAACACTTTGGAGCCTGCTTTGCCTGCAAACCAGCCATCAGTTCTCTTATTTGTGGATAGATCATCTGACTTGTTAGAGACTAGAATCAATGGCAAGGAAGCTCTTGATGCTTTTCGGGAGCTGGCACTGCACTATCACATGTCTGACCAGATTGGCAGTCAAAGTAGGGACAAGCATGAGAAATCCTCAGTCCAGCATTATCAAGCATTAAGGACCACATCTGGACATCCTAAATTAAAATTATCTCAGACAGTTCAGATTAGCAAACTAGAAGACAAGATGTCTACTTTCACAATTATAAATGAGGGCAAACAATTCACAGTGGAGAAGTTGGCTTTGGATCTAAAGGGTAATTCCTTGCAGGATATATTGGAAACTGTACTTAAGCAAAAGAAGAAAGCTAAGTTAAGCTCACTGGTGAAGGATTTGGGTTTCCAACTTTTGTCAGATGATATGGACATCAAACCAGAGAGTACATTGCCATTACCGATGGAATCTGAATCTGATACAGTTACAGAAGAGCCATATAAGGAGGGCCTTGCTACAAGTAGTACTGACTCAGACAGAGATCAACAGCTAGATGCAACAAGTATATCTACTGAACAGCATCCAGAAACTTCTAAAGTCATGGATACTGAAAAGTCGTCTCAGAACAATGAAGAAAAAACAGATTATGTTGATACAAGCAATCAGATCTTATCTGTAGATAGTGATCAACAGCTTGCAGATCATAAATGTGAAGATTTAGATGCAGAAGAAGATAGTAATTCACAGGGCAAAAAGTCAGCAGAGCAGCAGCTTCACTTTCAAGGCTTTAAGGGTTCATTTTTCTTTTCTGATGGTAATTGTCGGTTACTTCGAGCGCTGACTGGTAGGCCAAAATTTCCAGCCCTTGTAATAGTTGATCCCAATATGCAGCAGCATTATGTCTTCCCAGAGGAGACTAATGTCAATTATTCTTCACTGGTTGATTTCATTTCTGCCTTTCTTAATGGAAGTCTTCTTCCATATCAACAGTCTGAAACTGTACTTGAAAGCTCTAGGAAAGCCACTCAACCACCATTTGTTAATCTGGACTTCCATCAGGTGGATCCTATCCCTCGAGTGACAACTCACACTTTCTCTGAGCTGGTTGTTGGGTTCAATCAATCTGACAGTGATTCTTGGAATAAGGATGTCTTGGTTCTTTTTAGCAATAGATGGTGTGGTTTTTGCCAGCGAATGGAGTTAGTTGTTCATGAAGTATACCGAGCTATGAAGAATTATGCTAAGATGCTGAAAAGGGAATCAAAGAATGAGAAATCAACGTTTCATGATAATATGACAGGCGACGTGGAAAATGAAATGCTGGAGCTGCCATTAATGTACTTGCTGGATTGCACATTGAATGATTGCAGTTTGATACTGAAATCGATGAATCAGAGGGAGGTTTATCCTACTCTGGTGCTCTTTCCAGCAGAAAAGAAGCATGCTTTTCCTTATGAAGGAGATATGGCAGTCACTGAGGTTTTCAAGTTCATGGCTGATCATGGAAGTAACTCTCATCATCTTACCAGTGAAAAAGGAATCTTGTGGACTTTAGCTAAAAAGAGAGGAAGCAATCAAGATTTCTTTATGGTTCAGTCATCTGACGTTCATGAGCAGTCTAGGGATACACTTCATGAAGTCCTATTAACAAATGTTCATAAACCAGTAATTGAAGACAAGCAGATCAAGTCTCACATACCCCAAGGCTTGCATGAAGCACCCCCGAATGTGGTGGTTGGTTCTGTCCTAGTTGCTACTGATAAACTTCATAGTGTACACCCCTTTGATAAATCAGAGATTCTCATTGTGAAGGCAGATCAGGTTACTGGATTTCAAGGTTTAATTATCAACAAGCATATTAGATGGGATGCTCTGCCTGAATTGGGAGAAGGAGTTAAGATGTTAGTAGAAGCCCCTTTGTCCTTTGGAGGACCGCTTATAAAGGGTGGAATGCCTCTTGTCGCGTTAACTCAAAAATTTGTCAAGGATGAATATCCGGAAATCTTACCAGGCATTGCATTTCTTGATCCATCAGCAACAATTCAAAAAATCAAAGAGCTCAAGTTAGGAAATCAATCTGTCACTGACTACTGGTTTTTCTTTGGGTATTCAAGTTGGGGTAGGGACCAGCTCTTTGTTGAGATTGATAAAGGAGCTTGGAATTTAAGTGATGATGGTATGAGACATTTAGATTGGCCATCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179 Thioredoxin

Protein Analysis

1143

Amino Acids

128.53

Weight (kDa)

5.46

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 1003 - 1132 1e-10 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1496, 3164
AasI GACNNNNNNGTC 1 cut(s) 50
AccB7I CCANNNNNTGG 3 cut(s) 1077, 2284, 2419
AccI GTMKAC 2 cut(s) 1481, 2442
AccII CGCG 1 cut(s) 3188
AccIII TCCGGA 2 cut(s) 595, 3220
AciI CCGC 4 cut(s) 47, 195, 769, 3158
AclI AACGTT 1 cut(s) 2504
AclWI GGATC 6 cut(s) 259, 1545, 2096, 2282, 2295, 3245
AcoI YGGCCR 2 cut(s) 678, 3419
AcsI RAATTY 5 cut(s) 722, 1016, 2078, 3200, 3385
AcuI CTGAAG 6 cut(s) 123, 387, 404, 429, 608, 841
AfaI GTAC 9 cut(s) 112, 473, 494, 1578, 1672, 1750, 2226, 2564, 3000
AfeI AGCGCT 1 cut(s) 2061
AflII CTTAAG 1 cut(s) 1580
AflIII ACRYGT 1 cut(s) 1335
AhdI GACNNNNNGTC 1 cut(s) 1477
AjiI CACGTC 2 cut(s) 340, 2530
AjnI CCWGG 3 cut(s) 150, 159, 3232
AjuI GAANNNNNNNTTGG 2 cut(s) 1517, 1549
Alw21I GWGCWC 4 cut(s) 127, 159, 2646, 3285
AlwI GGATC 6 cut(s) 259, 1545, 2096, 2282, 2295, 3245
AlwNI CAGNNNCTG 1 cut(s) 2699
Ama87I CYCGRG 1 cut(s) 2298
Aor13HI TCCGGA 2 cut(s) 595, 3220
Aor51HI AGCGCT 1 cut(s) 2061
AoxI GGCC 5 cut(s) 678, 905, 1732, 2072, 3419
ApeKI GCWGC 9 cut(s) 115, 185, 440, 878, 1990, 1993, 2113, 2116, 2551
ApoI RAATTY 5 cut(s) 722, 1016, 2078, 3200, 3385
ArsI GACNNNNNNTTYG 4 cut(s) 624, 656, 3341, 3373
AseI ATTAAT 1 cut(s) 2558
Asp700I GAANNNNTTC 2 cut(s) 570, 611
AspLEI GCGC 2 cut(s) 520, 2062
AspS9I GGNCC 6 cut(s) 12, 309, 1410, 1732, 3155, 3349
AsuHPI GGTGA 5 cut(s) 326, 470, 575, 1202, 1627
AsuII TTCGAA 1 cut(s) 1118
AvaI CYCGRG 1 cut(s) 2298
AvaII GGWCC 5 cut(s) 12, 309, 1410, 3155, 3349
BalI TGGCCA 2 cut(s) 680, 3421
BamHI GGATCC 1 cut(s) 2287
BanII GRGCYC 1 cut(s) 3285
BbsI GAAGAC 5 cut(s) 104, 1476, 2119, 2189, 2904
Bbv12I GWGCWC 4 cut(s) 127, 159, 2646, 3285
BbvI GCAGC 9 cut(s) 102, 197, 427, 865, 2002, 2005, 2125, 2128, 2538
BceAI ACGGC 1 cut(s) 182
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BciT130I CCWGG 3 cut(s) 152, 161, 3234
BciVI GTATCC 3 cut(s) 30, 1822, 2842
BclI TGATCA 3 cut(s) 703, 1909, 2722
BfaI CTAG 6 cut(s) 1278, 1466, 1775, 2238, 2844, 2972
BfmI CTRYAG 1 cut(s) 1899
BfoI RGCGCY 1 cut(s) 2063
BfrI CTTAAG 1 cut(s) 1580
BfuI GTATCC 3 cut(s) 30, 1822, 2842
BglII AGATCT 2 cut(s) 1037, 1890
BmcAI AGTACT 1 cut(s) 1750
Bme1390I CCNGG 3 cut(s) 152, 161, 3234
Bme18I GGWCC 5 cut(s) 12, 309, 1410, 3155, 3349
BmeRI GACNNNNNGTC 1 cut(s) 1477
BmeT110I CYCGRG 1 cut(s) 2298
BmgBI CACGTC 2 cut(s) 340, 2530
BmgT120I GGNCC 6 cut(s) 12, 309, 1410, 1732, 3155, 3349
BmiI GGNNCC 3 cut(s) 1209, 2289, 3350
BmrFI CCNGG 3 cut(s) 152, 161, 3234
BmrI ACTGGG 2 cut(s) 77, 1104
BmsI GCATC 8 cut(s) 126, 926, 1294, 1768, 1813, 1936, 2460, 3082
BmuI ACTGGG 2 cut(s) 77, 1104
BoxI GACNNNNGTC 1 cut(s) 2144
BpiI GAAGAC 5 cut(s) 104, 1476, 2119, 2189, 2904
BplI GAGNNNNNCTC 4 cut(s) 362, 394, 1367, 1399
BpmI CTGGAG 2 cut(s) 1205, 2567
Bpu14I TTCGAA 1 cut(s) 1118
BpuEI CTTGAG 1 cut(s) 3269
Bsa29I ATCGAT 1 cut(s) 2609
BsaBI GATNNNNATC 2 cut(s) 1908, 3216
BsaI GGTCTC 1 cut(s) 1033
BsaJI CCNNGG 3 cut(s) 305, 842, 2926
BsaWI WCCGGW 3 cut(s) 311, 595, 3220
BsaXI ACNNNNNCTCC 2 cut(s) 3102, 3132
Bse3DI GCAATG 2 cut(s) 1673, 3237
Bse8I GATNNNNATC 2 cut(s) 1908, 3216
BseAI TCCGGA 2 cut(s) 595, 3220
BseBI CCWGG 3 cut(s) 152, 161, 3234
BseCI ATCGAT 1 cut(s) 2609
BseDI CCNNGG 3 cut(s) 305, 842, 2926
BseJI GATNNNNATC 2 cut(s) 1908, 3216
BseMI GCAATG 2 cut(s) 1673, 3237
BseRI GAGGAG 3 cut(s) 278, 902, 2150
BseXI GCAGC 9 cut(s) 102, 197, 427, 865, 2002, 2005, 2125, 2128, 2538
BsgI GTGCAG 3 cut(s) 101, 855, 1310
Bsh1236I CGCG 1 cut(s) 3188
Bsh1285I CGRYCG 1 cut(s) 435
BshFI GGCC 5 cut(s) 680, 907, 1734, 2074, 3421
BshVI ATCGAT 1 cut(s) 2609
BsiEI CGRYCG 1 cut(s) 435
BsiHKAI GWGCWC 4 cut(s) 127, 159, 2646, 3285
BsiHKCI CYCGRG 1 cut(s) 2298
BsiSI CCGG 5 cut(s) 27, 222, 312, 596, 3221
BslFI GGGAC 6 cut(s) 528, 712, 866, 1092, 1379, 3362
BsmBI CGTCTC 3 cut(s) 275, 330, 1848
BsmFI GGGAC 6 cut(s) 528, 712, 866, 1092, 1379, 3362
BsmI GAATGC 2 cut(s) 748, 3180
BsnI GGCC 5 cut(s) 680, 907, 1734, 2074, 3421
Bso31I GGTCTC 1 cut(s) 1033
BsoBI CYCGRG 1 cut(s) 2298
Bsp119I TTCGAA 1 cut(s) 1118
Bsp1286I GDGCHC 4 cut(s) 127, 159, 2646, 3285
Bsp13I TCCGGA 2 cut(s) 595, 3220
Bsp1407I TGTACA 1 cut(s) 2998
Bsp19I CCATGG 1 cut(s) 305
BspACI CCGC 4 cut(s) 47, 195, 769, 3158
BspANI GGCC 5 cut(s) 680, 907, 1734, 2074, 3421
BspDI ATCGAT 1 cut(s) 2609
BspEI TCCGGA 2 cut(s) 595, 3220
BspFNI CGCG 1 cut(s) 3188
BspHI TCATGA 6 cut(s) 330, 972, 2434, 2509, 2833, 2857
BspLI GGNNCC 3 cut(s) 1209, 2289, 3350
BspPI GGATC 6 cut(s) 259, 1545, 2096, 2282, 2295, 3245
BspQI GCTCTTC 1 cut(s) 1710
BspT104I TTCGAA 1 cut(s) 1118
BspTI CTTAAG 1 cut(s) 1580
BspTNI GGTCTC 1 cut(s) 1033
BsrDI GCAATG 2 cut(s) 1673, 3237
BsrGI TGTACA 1 cut(s) 2998
BssECI CCNNGG 3 cut(s) 305, 842, 2926
BssNAI GTATAC 1 cut(s) 2443
BssT1I CCWWGG 3 cut(s) 305, 842, 2926
Bst1107I GTATAC 1 cut(s) 2443
Bst2UI CCWGG 3 cut(s) 152, 161, 3234
Bst6I CTCTTC 8 cut(s) 99, 262, 296, 306, 363, 584, 594, 1710
BstAFI CTTAAG 1 cut(s) 1580
BstAUI TGTACA 1 cut(s) 2998
BstBI TTCGAA 1 cut(s) 1118
BstC8I GCNNGC 9 cut(s) 166, 380, 1212, 1221, 1320, 1920, 2414, 2667, 2933
BstDSI CCRYGG 1 cut(s) 305
BstENI CCTNNNNNAGG 1 cut(s) 2679
BstFNI CGCG 1 cut(s) 3188
BstH2I RGCGCY 1 cut(s) 2063
BstHHI GCGC 2 cut(s) 520, 2062
BstMCI CGRYCG 1 cut(s) 435
BstNI CCWGG 3 cut(s) 152, 161, 3234
BstNSI RCATGY 2 cut(s) 1339, 2669
BstPAI GACNNNNGTC 1 cut(s) 2144
BstSCI CCNGG 3 cut(s) 150, 159, 3232
BstSFI CTRYAG 1 cut(s) 1899
BstUI CGCG 1 cut(s) 3188
BstV1I GCAGC 9 cut(s) 102, 197, 427, 865, 2002, 2005, 2125, 2128, 2538
BstV2I GAAGAC 5 cut(s) 104, 1476, 2119, 2189, 2904
BstX2I RGATCY 5 cut(s) 264, 1037, 1537, 1890, 2287
BstXI CCANNNNNNTGG 1 cut(s) 85
BstYI RGATCY 5 cut(s) 264, 1037, 1537, 1890, 2287
BstZ17I GTATAC 1 cut(s) 2443
Bsu15I ATCGAT 1 cut(s) 2609
BsuI GTATCC 3 cut(s) 30, 1822, 2842
BsuRI GGCC 5 cut(s) 680, 907, 1734, 2074, 3421
BsuTUI ATCGAT 1 cut(s) 2609
BtgI CCRYGG 1 cut(s) 305
BtgZI GCGATG 1 cut(s) 149
BtrI CACGTC 2 cut(s) 340, 2530
BtsI GCAGTG 2 cut(s) 1092, 1322
Cac8I GCNNGC 9 cut(s) 166, 380, 1212, 1221, 1320, 1920, 2414, 2667, 2933
CaiI CAGNNNCTG 1 cut(s) 2699
CciI TCATGA 6 cut(s) 330, 972, 2434, 2509, 2833, 2857
CfoI GCGC 2 cut(s) 520, 2062
Cfr13I GGNCC 6 cut(s) 12, 309, 1410, 1732, 3155, 3349
ClaI ATCGAT 1 cut(s) 2609
CsiI ACCWGGT 1 cut(s) 150
Csp6I GTAC 9 cut(s) 111, 472, 493, 1577, 1671, 1749, 2225, 2563, 2999
CviQI GTAC 9 cut(s) 111, 472, 493, 1577, 1671, 1749, 2225, 2563, 2999
DraI TTTAAA 1 cut(s) 1158
DrdI GACNNNNNNGTC 1 cut(s) 50
DriI GACNNNNNGTC 1 cut(s) 1477
DseDI GACNNNNNNGTC 1 cut(s) 50
EaeI YGGCCR 2 cut(s) 678, 3419
Eam1104I CTCTTC 8 cut(s) 99, 262, 296, 306, 363, 584, 594, 1710
Eam1105I GACNNNNNGTC 1 cut(s) 1477
EarI CTCTTC 8 cut(s) 99, 262, 296, 306, 363, 584, 594, 1710
EciI GGCGGA 1 cut(s) 784
Ecl136II GAGCTC 1 cut(s) 3283
Eco130I CCWWGG 3 cut(s) 305, 842, 2926
Eco147I AGGCCT 1 cut(s) 907
Eco24I GRGCYC 1 cut(s) 3285
Eco31I GGTCTC 1 cut(s) 1033
Eco47I GGWCC 5 cut(s) 12, 309, 1410, 3155, 3349
Eco47III AGCGCT 1 cut(s) 2061
Eco53kI GAGCTC 1 cut(s) 3283
Eco57I CTGAAG 6 cut(s) 123, 387, 404, 429, 608, 841
Eco88I CYCGRG 1 cut(s) 2298
EcoICRI GAGCTC 1 cut(s) 3283
EcoNI CCTNNNNNAGG 1 cut(s) 2679
EcoO109I RGGNCCY 1 cut(s) 1732
EcoRII CCWGG 3 cut(s) 150, 159, 3232
EcoT14I CCWWGG 3 cut(s) 305, 842, 2926
EcoT38I GRGCYC 1 cut(s) 3285
ErhI CCWWGG 3 cut(s) 305, 842, 2926
Esp3I CGTCTC 3 cut(s) 275, 330, 1848
FalI AAGNNNNNCTT 4 cut(s) 236, 268, 2360, 2392
FaqI GGGAC 6 cut(s) 528, 712, 866, 1092, 1379, 3362
FauI CCCGC 1 cut(s) 54
FbaI TGATCA 3 cut(s) 703, 1909, 2722
FblI GTMKAC 2 cut(s) 1481, 2442
FriOI GRGCYC 1 cut(s) 3285
FspBI CTAG 6 cut(s) 1278, 1466, 1775, 2238, 2844, 2972
GlaI GCGC 2 cut(s) 519, 2061
GsuI CTGGAG 2 cut(s) 1205, 2567
HaeII RGCGCY 1 cut(s) 2063
HaeIII GGCC 5 cut(s) 680, 907, 1734, 2074, 3421
HapII CCGG 5 cut(s) 27, 222, 312, 596, 3221
HhaI GCGC 2 cut(s) 520, 2062
Hin6I GCGC 2 cut(s) 518, 2060
HinP1I GCGC 2 cut(s) 518, 2060
HincII GTYRAC 1 cut(s) 3192
HindII GTYRAC 1 cut(s) 3192
HindIII AAGCTT 2 cut(s) 609, 633
HpaI GTTAAC 1 cut(s) 3192
HpaII CCGG 5 cut(s) 27, 222, 312, 596, 3221
HphI GGTGA 5 cut(s) 326, 470, 575, 1202, 1627
Hpy166II GTNNAC 7 cut(s) 111, 1482, 2443, 2772, 2999, 3001, 3192
Hpy8I GTNNAC 7 cut(s) 111, 1482, 2443, 2772, 2999, 3001, 3192
Hpy99I CGWCG 2 cut(s) 145, 2531
HpyAV CCTTC 5 cut(s) 778, 1612, 2675, 3028, 3107
HpyCH4IV ACGT 4 cut(s) 339, 2504, 2529, 2829
HpySE526I ACGT 4 cut(s) 339, 2504, 2529, 2829
HspAI GCGC 2 cut(s) 518, 2060
Kpn2I TCCGGA 2 cut(s) 595, 3220
Ksp22I TGATCA 3 cut(s) 703, 1909, 2722
KspAI GTTAAC 1 cut(s) 3192
LguI GCTCTTC 1 cut(s) 1710
LmnI GCTCC 7 cut(s) 162, 224, 1180, 1207, 1315, 2548, 3377
Lsp1109I GCAGC 9 cut(s) 102, 197, 427, 865, 2002, 2005, 2125, 2128, 2538
LweI GCATC 8 cut(s) 126, 926, 1294, 1768, 1813, 1936, 2460, 3082
MabI ACCWGGT 1 cut(s) 150
MaeI CTAG 6 cut(s) 1278, 1466, 1775, 2238, 2844, 2972
MaeII ACGT 4 cut(s) 339, 2504, 2529, 2829
MaeIII GTNAC 8 cut(s) 295, 1708, 2049, 2302, 2695, 2732, 3046, 3304
MflI RGATCY 5 cut(s) 264, 1037, 1537, 1890, 2287
MhlI GDGCHC 4 cut(s) 127, 159, 2646, 3285
MlsI TGGCCA 2 cut(s) 680, 3421
MluNI TGGCCA 2 cut(s) 680, 3421
MlyI GAGTC 4 cut(s) 601, 658, 947, 1748
MmeI TCCRAC 2 cut(s) 79, 1657
Mox20I TGGCCA 2 cut(s) 680, 3421
MroI TCCGGA 2 cut(s) 595, 3220
MroXI GAANNNNTTC 2 cut(s) 570, 611
MscI TGGCCA 2 cut(s) 680, 3421
MslI CAYNNNNRTG 1 cut(s) 1932
Msp20I TGGCCA 2 cut(s) 680, 3421
MspCI CTTAAG 1 cut(s) 1580
MspI CCGG 5 cut(s) 27, 222, 312, 596, 3221
MspR9I CCNGG 3 cut(s) 152, 161, 3234
Mva1269I GAATGC 2 cut(s) 748, 3180
MvaI CCWGG 3 cut(s) 152, 161, 3234
MvnI CGCG 1 cut(s) 3188
NcoI CCATGG 1 cut(s) 305
NlaIV GGNNCC 3 cut(s) 1209, 2289, 3350
NmuCI GTSAC 4 cut(s) 295, 2302, 2695, 3304
NspI RCATGY 2 cut(s) 1339, 2669
NspV TTCGAA 1 cut(s) 1118
PaeI GCATGC 1 cut(s) 2669
PaeR7I CTCGAG 1 cut(s) 2298
PagI TCATGA 6 cut(s) 330, 972, 2434, 2509, 2833, 2857
PceI AGGCCT 1 cut(s) 907
PciI ACATGT 1 cut(s) 1335
PciSI GCTCTTC 1 cut(s) 1710
PcsI WCGNNNNNNNCGW 1 cut(s) 2053
PctI GAATGC 2 cut(s) 748, 3180
PdmI GAANNNNTTC 2 cut(s) 570, 611
PflMI CCANNNNNTGG 3 cut(s) 1077, 2284, 2419
Ple19I CGATCG 1 cut(s) 435
PleI GAGTC 4 cut(s) 600, 658, 947, 1748
PpsI GAGTC 4 cut(s) 600, 658, 947, 1748
PscI ACATGT 1 cut(s) 1335
PshAI GACNNNNGTC 1 cut(s) 2144
PshBI ATTAAT 1 cut(s) 2558
PsiI TTATAA 2 cut(s) 1496, 3164
Psp124BI GAGCTC 1 cut(s) 3285
Psp1406I AACGTT 1 cut(s) 2504
Psp6I CCWGG 3 cut(s) 150, 159, 3232
PspGI CCWGG 3 cut(s) 150, 159, 3232
PspN4I GGNNCC 3 cut(s) 1209, 2289, 3350
PspPI GGNCC 6 cut(s) 12, 309, 1410, 1732, 3155, 3349
PspXI VCTCGAGB 1 cut(s) 2298
PstNI CAGNNNCTG 1 cut(s) 2699
PsuI RGATCY 5 cut(s) 264, 1037, 1537, 1890, 2287
PvuI CGATCG 1 cut(s) 435
RsaI GTAC 9 cut(s) 112, 473, 494, 1578, 1672, 1750, 2226, 2564, 3000
RsaNI GTAC 9 cut(s) 111, 472, 493, 1577, 1671, 1749, 2225, 2563, 2999
RseI CAYNNNNRTG 1 cut(s) 1932
SacI GAGCTC 1 cut(s) 3285
SapI GCTCTTC 1 cut(s) 1710
Sau96I GGNCC 6 cut(s) 12, 309, 1410, 1732, 3155, 3349
ScaI AGTACT 1 cut(s) 1750
SchI GAGTC 4 cut(s) 601, 658, 947, 1748
ScrFI CCNGG 3 cut(s) 152, 161, 3234
SduI GDGCHC 4 cut(s) 127, 159, 2646, 3285
SexAI ACCWGGT 1 cut(s) 150
SfaNI GCATC 8 cut(s) 126, 926, 1294, 1768, 1813, 1936, 2460, 3082
SfcI CTRYAG 1 cut(s) 1899
Sfr274I CTCGAG 1 cut(s) 2298
SfuI TTCGAA 1 cut(s) 1118
SinI GGWCC 5 cut(s) 12, 309, 1410, 3155, 3349
SlaI CTCGAG 1 cut(s) 2298
SmiMI CAYNNNNRTG 1 cut(s) 1932
SmlI CTYRAG 3 cut(s) 1580, 2298, 3284
SmoI CTYRAG 3 cut(s) 1580, 2298, 3284
SphI GCATGC 1 cut(s) 2669
SseBI AGGCCT 1 cut(s) 907
SsiI CCGC 4 cut(s) 47, 195, 769, 3158
SspMI CTAG 6 cut(s) 1278, 1466, 1775, 2238, 2844, 2972
SstI GAGCTC 1 cut(s) 3285
StuI AGGCCT 1 cut(s) 907
StyD4I CCNGG 3 cut(s) 150, 159, 3232
StyI CCWWGG 3 cut(s) 305, 842, 2926
TaiI ACGT 4 cut(s) 342, 2507, 2532, 2832
TaqI TCGA 7 cut(s) 322, 361, 514, 1118, 2056, 2299, 2609
TatI WGTACW 7 cut(s) 471, 1576, 1670, 1748, 2224, 2562, 2998
TauI GCSGC 1 cut(s) 197
TseFI GTSAC 4 cut(s) 295, 2302, 2695, 3304
TseI GCWGC 9 cut(s) 115, 185, 440, 878, 1990, 1993, 2113, 2116, 2551
Tsp45I GTSAC 4 cut(s) 295, 2302, 2695, 3304
TspGWI ACGGA 1 cut(s) 257
Van91I CCANNNNNTGG 3 cut(s) 1077, 2284, 2419
Vha464I CTTAAG 1 cut(s) 1580
VpaK11BI GGWCC 5 cut(s) 12, 309, 1410, 3155, 3349
VspI ATTAAT 1 cut(s) 2558
XagI CCTNNNNNAGG 1 cut(s) 2679
XapI RAATTY 5 cut(s) 722, 1016, 2078, 3200, 3385
XceI RCATGY 2 cut(s) 1339, 2669
XcmI CCANNNNNNNNNTGG 1 cut(s) 1078
XhoI CTCGAG 1 cut(s) 2298
XmiI GTMKAC 2 cut(s) 1481, 2442
XmnI GAANNNNTTC 2 cut(s) 570, 611
XspI CTAG 6 cut(s) 1278, 1466, 1775, 2238, 2844, 2972
ZrmI AGTACT 1 cut(s) 1750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.