MD13G1007100.v1.1

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009991
Physical Location & Seq
Forward (+)
460887 .. 465588
4702 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1007100.v1.1.491

Sequence Viewer

Length: 3345 bp
ATGGCGACGACGACAACGACGACGACGACGACGACGACGACGACGACGAAGAATAAGAAAGTGGTGTTCTTAGCTTGCTGGATATTCCTCGCGGCAGCAATCCCAGCCGTTGAATCTAAATCAGACGGCGTAGGCGAATGGCAACTCCTGACAAAGCAAAATTTCTCCTCCCAGATCCGCCTCCACCCTCACATCCTCCTCATCGTCACTCTCCCATGGTCGGGAGAATCTCGATCGCTCATGAAAGATGTAGCTCAATTGGTTACCGATAATCATGAAGATTTCAGCTCATTGAGATTGAGGTTTATGTACAGAAACGCAGAGAAGGTGTTGGCGGACGCCATTGGAGCTGCCACTGGAGCAGAGGAAACAACAGTTTTGTACTATCACAATTCTGTGTCTTACAAGTATGGCGGAAGACTTAGGGCGCAAAATGTATTGGCTTCGCTCCGTCCGTATGTGGCGGTTGGACCAGAAGAGCTTCCGTTCAAGTCCCTGAAAACTCCGGAGGAACTGAAAGAGTTCGTTGATTCAACTGACAAAGCTTTGATTCTGTTTGAGTTTTGCGAATGGACTTCGAAATTGCTTGCCAGGAGGAAGATGAATGGAACTGATCGAAACGGTTCTGCTCTGCAAGGGAATTTTCTTGGATTCAACATCAGCAGAGAGGCGAATTCATCACCAGCACACTTGGGGAAGAATATCCAGAAGGGTATTGAAAGTGCAAAGATGTGCGGTGTTGATTATGGGCTTGGTGGTGTTCCTTGGCTTGGGGACTTCAGCTCGGTAAATGACAGTGCTTCTTTTGAGGAGTCTGAGCAAATGAGCTCATTCTGTAATCGCGAAGAATATCAGCTGTTTGACTCTTTCCTCTCAAAGTTCACAACCGTTGCACGGGAGTTCTTTCTACCTCCCGAAAGGTATAAATTTGGTCTGGTCTCAGAAAGATCGATGCTCTCAACTTTTGGGATTGAAGATTCCAGTACATGGTTGGCAGTTCTGTACTTATCTGGATGCCCCGGCTGTTCAAAGATTATTAAGAAAGAGGAGGACCTCAAAAATGCTTTACAGATGGATAATTTGGTTGTTACGGAGTTGGAAGGTCTTGGAAACACTTTGAAGCCTGCTTTACCTGCAAACCAACCATCAGCACTCCTATTTGTGGATAGATCATCTGACTTGTCAGAGACTAGAATAAAATGTAAGAAAGCTCTTGATGCTTTTCGAGAGCTGGCACTGCACTATCATATTTCACGCCAAAGTGGTGGGCAATATGGAGACAAGTCTGAGAAATCCTCGGTCCAAGATTATCAAGCATTAAGGAGTACATCTGGATATCCAAAACTAAAGTTGTCTCAGACAATCAAATTAAACGACAAAACGTCTACTTTCATGATTGTAGATGAAGGAAAGCAAGTCACTTTGGATAATATAGCTTTGGATCTACAGGGTAGTTCCTTGAAGGAGATCTTGGATATCGTACTTAAGCAAAAGAACAAAGGTAAATTAAGCTCGCTTGCAAAAGAGTTAGGTTTCCAACTTTTATCAGATGACATGGACATCAGGTTAGTGAATACATTGCCAGTACAGAAAGAACTTCAGTCTGATCAACTTACAGAAGAGCTGTCTAAGGAAGGCCTTGTGACAAGTAGTGTTAATTCAGACAAGGATCAATTGCCACACAGGACTAGTGTATCTGCTGAAGAGCATCTGGAAACTTCGGAAGTCACAGATTCTGACATCTTTTCTCAGACTGATGAAGAGAAAACAGCATATGTTGGTACAAGTAAGCAGTTCTTATCTGCAGATAGTGAACAACATCTTGTAGATCATAAACTTGATAGTACTGAAGATATAAAGGTCGATGAAGAAAGTTCTTCGCAGGAAGACAAATCAGGGGAGCAACAGCTTTGCTCTCAAGGTTTTGAGGGCTCTTTTTTCTTTTCTGATGGTAATTATCGATTACTTAATAGTCTCACTGGTGGATCAAAAATTCCTGCCTTGGTGATAGTTGATCCCATTGCCCAGCAACATTTTGTCTTCTCAGAAGAGACTGATCTTAGCTATCCTTCACTGGCTAATTTTCTTTCTGGGTTTGTTAATGGAAGTCTTCTTCCATATCAACAGTCTGAATCTGTTCTTCAAAGCTCTAGGGAGGCCACTCAACCACCATTTGTTAATCTGGATTTTCGTGAAGTGGATTCTGTCCCTCGAGTTACAAGTCATACCTTCACCGACCAGGTGATTGGGTTTAATCAATCAGACACTGACGCTTGGAATAAGGATGTCTTGGTCCTTTTTAGCAATAGATGGTGTGGATTTTGCCAGAGAATGGAATTGGTTGTTCGTGAAATATATCGGGCTATGAGGGACTATATTAAGTTGCTGAAAAGTGGATCAAAGAATGAGAAAACAAGGTTTCACGATGGCGACTTGAAGGATGAAATGCTGAAGCTTCCATTAGTATACTTGCTTGATTGCACCTTGAATGATTGCAGTTTGATATTGAAATCAATGAATCAGAGGGAAGTTTATCCTGCTCTCGTGCTCTTTCCAGCTGAAAAGAAGAATGCTGTCCTTTATGAAGGGGATATGGCAGTCACTGAAATCTTCGAGTTTATGGCCGATCGTGGAAGTAACTCGGATGATCTTATCAGTGAGAAGGGAAGCTTATGGACTGTAGCAAAAAAGTGGGGAAGGAATCAAAATTTATTTAAGGTCCAGTCTTCTGACATTCACGAGGGAGCTCCCTTTGAAAAGGACACACTTCATGAAATACTTTTAACACAGACGCATAAACAAGGCATTAGAGACAATCAGCCCGAGTCACACACATCACAAGGTTTGCAGGAAGCAGCCCTGCGTGTGGTGACCGGTTCCACTTTAGTTGCTACTGATAAACTTCTTACTGTACATCCCTTTGATAAATCAGAGGTTCTTATCGTCAAGGCAGATCGAGTTTCTGGATTTGAAGGTCTGATTATTAACAAGAATATCAAATGGGATGTTCTTCTTGAATTGGACAAAGGGCTGGAGATGCTATCCGAGGCCCCTCTGTCTTTTGGAGGACCACTTGTAAAGGTTGGAATGCCTCTAGTTGCATTAACTCGAAGATTTGTTACGAATGAGTATCCGGAAGTCCTGCCGGGTGTTTACTTTCTTGATCAATCAGCAACACTTCGTAAAATCAAAGAGATCAAGTTAGGAAATCAATCTGTCAGCGACCACTGGTTTTTCTATGGGTATTCAAGTTGGGGTTGGGACCAACTCTTTGATGAGATTGCTGAAGGAGCTTGGGACTTGAGTGATGATGGTACGAGGCATTTGGATTGGCCATCGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179 Thioredoxin

Protein Analysis

1115

Amino Acids

124.66

Weight (kDa)

5.12

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 973 - 1103 2.2e-11 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1141
AccB7I CCANNNNNTGG 2 cut(s) 987, 2332
AccI GTMKAC 2 cut(s) 1385, 2466
AccII CGCG 2 cut(s) 92, 843
AccIII TCCGGA 2 cut(s) 505, 3133
AciI CCGC 6 cut(s) 92, 178, 335, 414, 464, 735
AclWI GGATC 6 cut(s) 169, 1449, 1677, 1993, 2009, 2404
AcoI YGGCCR 2 cut(s) 2622, 3332
AcsI RAATTY 6 cut(s) 160, 640, 673, 926, 1992, 2707
AcuI CTGAAG 6 cut(s) 763, 1583, 1722, 1869, 2471, 3306
AcyI GRCGYC 1 cut(s) 339
AfiI CCNNNNNNNGG 8 cut(s) 220, 221, 770, 894, 987, 1162, 2332, 2866
AflII CTTAAG 1 cut(s) 1484
AgeI ACCGGT 1 cut(s) 2873
AhdI GACNNNNNGTC 1 cut(s) 1381
AhlI ACTAGT 1 cut(s) 1688
AjnI CCWGG 2 cut(s) 590, 2238
AjuI GAANNNNNNNTTGG 2 cut(s) 1454, 1486
Alw21I GWGCWC 3 cut(s) 830, 2550, 2749
Alw26I GTCTC 7 cut(s) 943, 1181, 1272, 1359, 1979, 2045, 2805
AlwI GGATC 6 cut(s) 169, 1449, 1677, 1993, 2009, 2404
AlwNI CAGNNNCTG 3 cut(s) 1736, 2267, 2603
Ama87I CYCGRG 2 cut(s) 2211, 2822
Aor13HI TCCGGA 2 cut(s) 505, 3133
AoxI GGCC 5 cut(s) 1636, 2157, 2622, 3048, 3332
ApeKI GCWGC 3 cut(s) 95, 350, 2855
ApoI RAATTY 6 cut(s) 160, 640, 673, 926, 1992, 2707
ArsI GACNNNNNNTTYG 2 cut(s) 3254, 3286
AsiGI ACCGGT 1 cut(s) 2873
Asp700I GAANNNNTTC 4 cut(s) 480, 521, 622, 2136
AspLEI GCGC 1 cut(s) 430
AspS9I GGNCC 8 cut(s) 470, 1051, 1300, 2293, 2719, 3049, 3068, 3262
AsuC2I CCSGG 2 cut(s) 1020, 3147
AsuHPI GGTGA 5 cut(s) 672, 2017, 2224, 2254, 2881
AsuII TTCGAA 1 cut(s) 578
AvaI CYCGRG 2 cut(s) 2211, 2822
AvaII GGWCC 7 cut(s) 470, 1051, 1300, 2293, 2719, 3068, 3262
BaeI ACNNNNGTAYC 2 cut(s) 1677, 1710
BalI TGGCCA 1 cut(s) 3334
BanII GRGCYC 3 cut(s) 830, 1934, 2749
BarI GAAGNNNNNNTAC 2 cut(s) 3103, 3135
BauI CACGAG 2 cut(s) 2543, 2738
BbsI GAAGAC 5 cut(s) 424, 1893, 2032, 2102, 2718
Bbv12I GWGCWC 3 cut(s) 830, 2550, 2749
BbvI GCAGC 3 cut(s) 107, 337, 2867
BccI CCATC 7 cut(s) 1066, 1153, 1943, 2304, 2420, 3305, 3343
BceAI ACGGC 2 cut(s) 92, 142
BciT130I CCWGG 2 cut(s) 592, 2240
BciVI GTATCC 1 cut(s) 3141
BclI TGATCA 2 cut(s) 1606, 3163
BcnI CCSGG 2 cut(s) 1020, 3147
BcoDI GTCTC 7 cut(s) 943, 1181, 1272, 1359, 1979, 2045, 2805
BcuI ACTAGT 1 cut(s) 1688
BfaI CTAG 4 cut(s) 1191, 1689, 2151, 3095
BfmI CTRYAG 3 cut(s) 1445, 1803, 2679
BfrI CTTAAG 1 cut(s) 1484
BfuAI ACCTGC 1 cut(s) 1141
BfuI GTATCC 1 cut(s) 3141
BglII AGATCT 1 cut(s) 1467
BisI GCNGC 4 cut(s) 93, 96, 351, 2856
BlsI GCNGC 4 cut(s) 94, 97, 352, 2857
BmcAI AGTACT 1 cut(s) 1846
Bme1390I CCNGG 4 cut(s) 592, 1020, 2240, 3147
Bme18I GGWCC 7 cut(s) 470, 1051, 1300, 2293, 2719, 3068, 3262
BmeRI GACNNNNNGTC 1 cut(s) 1381
BmeT110I CYCGRG 2 cut(s) 2211, 2822
BmgT120I GGNCC 8 cut(s) 470, 1051, 1300, 2293, 2719, 3049, 3068, 3262
BmiI GGNNCC 3 cut(s) 2878, 3051, 3263
BmrFI CCNGG 4 cut(s) 592, 1020, 2240, 3147
BmsI GCATC 5 cut(s) 942, 1004, 1207, 1717, 3027
BpiI GAAGAC 5 cut(s) 424, 1893, 2032, 2102, 2718
BplI GAGNNNNNCTC 2 cut(s) 1280, 1312
BpmI CTGGAG 2 cut(s) 378, 3053
Bpu14I TTCGAA 1 cut(s) 578
BpuEI CTTGAG 2 cut(s) 1902, 3322
BpuMI CCSGG 2 cut(s) 1020, 3147
Bsa29I ATCGAT 2 cut(s) 950, 1960
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 943
BsaJI CCNNGG 6 cut(s) 215, 764, 1018, 1296, 2001, 3045
BsaWI WCCGGW 3 cut(s) 505, 2873, 3133
Bsc4I CCNNNNNNNGG 8 cut(s) 220, 221, 770, 894, 987, 1162, 2332, 2866
Bse118I RCCGGY 1 cut(s) 2873
Bse1I ACTGG 7 cut(s) 361, 981, 1583, 1984, 2079, 2722, 3233
Bse3DI GCAATG 2 cut(s) 1577, 2019
BseAI TCCGGA 2 cut(s) 505, 3133
BseBI CCWGG 2 cut(s) 592, 2240
BseCI ATCGAT 2 cut(s) 950, 1960
BseDI CCNNGG 6 cut(s) 215, 764, 1018, 1296, 2001, 3045
BseGI GGATG 7 cut(s) 192, 1019, 2290, 2446, 2650, 2914, 3010
BseLI CCNNNNNNNGG 8 cut(s) 220, 221, 770, 894, 987, 1162, 2332, 2866
BseMI GCAATG 2 cut(s) 1577, 2019
BseMII CTCAG 6 cut(s) 807, 954, 1278, 1370, 1763, 2058
BseNI ACTGG 7 cut(s) 361, 981, 1583, 1984, 2079, 2722, 3233
BseRI GAGGAG 4 cut(s) 157, 188, 824, 1061
BseXI GCAGC 3 cut(s) 107, 337, 2867
BseYI CCCAGC 2 cut(s) 103, 2025
BsgI GTGCAG 1 cut(s) 1223
Bsh1236I CGCG 2 cut(s) 92, 843
Bsh1285I CGRYCG 2 cut(s) 236, 2629
BshFI GGCC 5 cut(s) 1638, 2159, 2624, 3050, 3334
BshTI ACCGGT 1 cut(s) 2873
BshVI ATCGAT 2 cut(s) 950, 1960
BsiEI CGRYCG 2 cut(s) 236, 2629
BsiHKAI GWGCWC 3 cut(s) 830, 2550, 2749
BsiHKCI CYCGRG 2 cut(s) 2211, 2822
BsiSI CCGG 5 cut(s) 506, 1020, 2874, 3134, 3146
BslFI GGGAC 6 cut(s) 478, 788, 2192, 2384, 3275, 3311
BslI CCNNNNNNNGG 8 cut(s) 220, 221, 770, 894, 987, 1162, 2332, 2866
BsmAI GTCTC 7 cut(s) 943, 1181, 1272, 1359, 1979, 2045, 2805
BsmFI GGGAC 6 cut(s) 478, 788, 2192, 2384, 3275, 3311
BsmI GAATGC 2 cut(s) 2575, 3093
BsnI GGCC 5 cut(s) 1638, 2159, 2624, 3050, 3334
Bso31I GGTCTC 1 cut(s) 943
BsoBI CYCGRG 2 cut(s) 2211, 2822
Bsp119I TTCGAA 1 cut(s) 578
Bsp1286I GDGCHC 4 cut(s) 830, 1934, 2550, 2749
Bsp13I TCCGGA 2 cut(s) 505, 3133
Bsp1407I TGTACA 2 cut(s) 309, 2911
Bsp19I CCATGG 1 cut(s) 215
Bsp68I TCGCGA 1 cut(s) 843
BspACI CCGC 6 cut(s) 92, 178, 335, 414, 464, 735
BspANI GGCC 5 cut(s) 1638, 2159, 2624, 3050, 3334
BspCNI CTCAG 6 cut(s) 808, 953, 1279, 1369, 1762, 2057
BspDI ATCGAT 2 cut(s) 950, 1960
BspEI TCCGGA 2 cut(s) 505, 3133
BspFNI CGCG 2 cut(s) 92, 843
BspHI TCATGA 4 cut(s) 240, 274, 1392, 2770
BspLI GGNNCC 3 cut(s) 2878, 3051, 3263
BspMAI CTGCAG 1 cut(s) 1807
BspMI ACCTGC 1 cut(s) 1141
BspPI GGATC 6 cut(s) 169, 1449, 1677, 1993, 2009, 2404
BspQI GCTCTTC 3 cut(s) 471, 1614, 1698
BspT104I TTCGAA 1 cut(s) 578
BspTI CTTAAG 1 cut(s) 1484
BspTNI GGTCTC 1 cut(s) 943
BsrDI GCAATG 2 cut(s) 1577, 2019
BsrFI RCCGGY 1 cut(s) 2873
BsrGI TGTACA 2 cut(s) 309, 2911
BsrI ACTGG 7 cut(s) 361, 981, 1583, 1984, 2079, 2722, 3233
BssAI RCCGGY 1 cut(s) 2873
BssECI CCNNGG 6 cut(s) 215, 764, 1018, 1296, 2001, 3045
BssNAI GTATAC 1 cut(s) 2467
BssNI GRCGYC 1 cut(s) 339
BssSI CACGAG 2 cut(s) 2543, 2738
BssT1I CCWWGG 3 cut(s) 215, 764, 2001
Bst1107I GTATAC 1 cut(s) 2467
Bst2BI CACGAG 2 cut(s) 2543, 2738
Bst2UI CCWGG 2 cut(s) 592, 2240
Bst4CI ACNGT 7 cut(s) 376, 623, 797, 889, 2127, 2680, 2911
Bst6I CTCTTC 5 cut(s) 471, 1614, 1698, 1755, 2043
BstACI GRCGYC 1 cut(s) 339
BstAFI CTTAAG 1 cut(s) 1484
BstAUI TGTACA 2 cut(s) 309, 2911
BstBI TTCGAA 1 cut(s) 578
BstC8I GCNNGC 6 cut(s) 76, 588, 1125, 1233, 1514, 1518
BstDSI CCRYGG 1 cut(s) 215
BstEII GGTNACC 2 cut(s) 262, 2869
BstF5I GGATG 7 cut(s) 192, 1019, 2290, 2446, 2650, 2914, 3010
BstFNI CGCG 2 cut(s) 92, 843
BstHHI GCGC 1 cut(s) 430
BstMAI GTCTC 7 cut(s) 943, 1181, 1272, 1359, 1979, 2045, 2805
BstMCI CGRYCG 2 cut(s) 236, 2629
BstMWI GCNNNNNNNGC 8 cut(s) 104, 347, 359, 1133, 1217, 1237, 3037, 3290
BstNI CCWGG 2 cut(s) 592, 2240
BstPI GGTNACC 2 cut(s) 262, 2869
BstSCI CCNGG 4 cut(s) 590, 1018, 2238, 3145
BstSFI CTRYAG 3 cut(s) 1445, 1803, 2679
BstUI CGCG 2 cut(s) 92, 843
BstV1I GCAGC 3 cut(s) 107, 337, 2867
BstV2I GAAGAC 5 cut(s) 424, 1893, 2032, 2102, 2718
BstX2I RGATCY 3 cut(s) 174, 1441, 1467
BstXI CCANNNNNNTGG 2 cut(s) 1265, 2246
BstYI RGATCY 3 cut(s) 174, 1441, 1467
BstZ17I GTATAC 1 cut(s) 2467
Bsu15I ATCGAT 2 cut(s) 950, 1960
BsuI GTATCC 1 cut(s) 3141
BsuRI GGCC 5 cut(s) 1638, 2159, 2624, 3050, 3334
BsuTUI ATCGAT 2 cut(s) 950, 1960
BtgI CCRYGG 1 cut(s) 215
BtsCI GGATG 7 cut(s) 192, 1019, 2290, 2446, 2650, 2914, 3010
BtsI GCAGTG 1 cut(s) 1235
BtsIMutI CAGTG 9 cut(s) 354, 802, 1235, 1977, 2072, 2265, 2601, 2662, 3226
BtuMI TCGCGA 1 cut(s) 843
BveI ACCTGC 1 cut(s) 1141
Cac8I GCNNGC 6 cut(s) 76, 588, 1125, 1233, 1514, 1518
CaiI CAGNNNCTG 3 cut(s) 1736, 2267, 2603
CciI TCATGA 4 cut(s) 240, 274, 1392, 2770
CfoI GCGC 1 cut(s) 430
Cfr10I RCCGGY 1 cut(s) 2873
Cfr13I GGNCC 8 cut(s) 470, 1051, 1300, 2293, 2719, 3049, 3068, 3262
ClaI ATCGAT 2 cut(s) 950, 1960
CseI GACGC 3 cut(s) 347, 2279, 2800
CsiI ACCWGGT 1 cut(s) 2238
CspAI ACCGGT 1 cut(s) 2873
CspCI CAANNNNNGTGG 2 cut(s) 2869, 2904
CviAII CATG 7 cut(s) 216, 241, 275, 987, 1393, 1555, 2771
DriI GACNNNNNGTC 1 cut(s) 1381
EaeI YGGCCR 2 cut(s) 2622, 3332
Eam1104I CTCTTC 5 cut(s) 471, 1614, 1698, 1755, 2043
Eam1105I GACNNNNNGTC 1 cut(s) 1381
EarI CTCTTC 5 cut(s) 471, 1614, 1698, 1755, 2043
EciI GGCGGA 3 cut(s) 167, 350, 429
Ecl136II GAGCTC 2 cut(s) 828, 2747
Eco130I CCWWGG 3 cut(s) 215, 764, 2001
Eco147I AGGCCT 1 cut(s) 1638
Eco24I GRGCYC 3 cut(s) 830, 1934, 2749
Eco31I GGTCTC 1 cut(s) 943
Eco32I GATATC 2 cut(s) 1337, 1477
Eco47I GGWCC 7 cut(s) 470, 1051, 1300, 2293, 2719, 3068, 3262
Eco53kI GAGCTC 2 cut(s) 828, 2747
Eco57I CTGAAG 6 cut(s) 763, 1583, 1722, 1869, 2471, 3306
Eco88I CYCGRG 2 cut(s) 2211, 2822
Eco91I GGTNACC 2 cut(s) 262, 2869
EcoICRI GAGCTC 2 cut(s) 828, 2747
EcoO109I RGGNCCY 2 cut(s) 1051, 3049
EcoO65I GGTNACC 2 cut(s) 262, 2869
EcoRI GAATTC 1 cut(s) 673
EcoRII CCWGG 2 cut(s) 590, 2238
EcoRV GATATC 2 cut(s) 1337, 1477
EcoT14I CCWWGG 3 cut(s) 215, 764, 2001
EcoT38I GRGCYC 3 cut(s) 830, 1934, 2749
ErhI CCWWGG 3 cut(s) 215, 764, 2001
FaeI CATG 7 cut(s) 219, 244, 278, 990, 1396, 1558, 2774
FalI AAGNNNNNCTT 8 cut(s) 1454, 1486, 1781, 1813, 2273, 2305, 2654, 2686
FaqI GGGAC 6 cut(s) 478, 788, 2192, 2384, 3275, 3311
FatI CATG 7 cut(s) 215, 240, 274, 986, 1392, 1554, 2770
FauNDI CATATG 1 cut(s) 1774
FbaI TGATCA 2 cut(s) 1606, 3163
FblI GTMKAC 2 cut(s) 1385, 2466
Fnu4HI GCNGC 4 cut(s) 93, 96, 351, 2856
FokI GGATG 7 cut(s) 179, 1026, 2297, 2453, 2657, 2901, 3017
FriOI GRGCYC 3 cut(s) 830, 1934, 2749
Fsp4HI GCNGC 4 cut(s) 93, 96, 351, 2856
FspBI CTAG 4 cut(s) 1191, 1689, 2151, 3095
GlaI GCGC 1 cut(s) 429
GluI GCNGC 4 cut(s) 93, 96, 351, 2856
GsaI CCCAGC 2 cut(s) 107, 2029
GsuI CTGGAG 2 cut(s) 378, 3053
HaeIII GGCC 5 cut(s) 1638, 2159, 2624, 3050, 3334
HapII CCGG 5 cut(s) 506, 1020, 2874, 3134, 3146
HgaI GACGC 3 cut(s) 347, 2279, 2800
HhaI GCGC 1 cut(s) 430
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 7 cut(s) 219, 244, 278, 990, 1396, 1558, 2774
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HindIII AAGCTT 3 cut(s) 543, 2453, 2668
HpaII CCGG 5 cut(s) 506, 1020, 2874, 3134, 3146
HphI GGTGA 5 cut(s) 672, 2017, 2224, 2254, 2881
Hpy166II GTNNAC 5 cut(s) 882, 1386, 1814, 2467, 3154
Hpy8I GTNNAC 5 cut(s) 882, 1386, 1814, 2467, 3154
HpyCH4III ACNGT 7 cut(s) 376, 623, 797, 889, 2127, 2680, 2911
HpyCH4IV ACGT 1 cut(s) 1382
HpyF10VI GCNNNNNNNGC 8 cut(s) 104, 347, 359, 1133, 1217, 1237, 3037, 3290
HpySE526I ACGT 1 cut(s) 1382
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 7 cut(s) 219, 244, 278, 990, 1396, 1558, 2774
HspAI GCGC 1 cut(s) 428
Kpn2I TCCGGA 2 cut(s) 505, 3133
Ksp22I TGATCA 2 cut(s) 1606, 3163
LguI GCTCTTC 3 cut(s) 471, 1614, 1698
LmnI GCTCC 7 cut(s) 347, 359, 453, 1900, 2744, 2752, 3290
Lsp1109I GCAGC 3 cut(s) 107, 337, 2867
LweI GCATC 5 cut(s) 942, 1004, 1207, 1717, 3027
MabI ACCWGGT 1 cut(s) 2238
MaeI CTAG 4 cut(s) 1191, 1689, 2151, 3095
MaeII ACGT 1 cut(s) 1382
MfeI CAATTG 2 cut(s) 257, 1673
MflI RGATCY 3 cut(s) 174, 1441, 1467
MhlI GDGCHC 4 cut(s) 830, 1934, 2550, 2749
MlsI TGGCCA 1 cut(s) 3334
MluNI TGGCCA 1 cut(s) 3334
MlyI GAGTC 3 cut(s) 821, 857, 2834
MmeI TCCRAC 4 cut(s) 448, 1077, 1561, 3064
Mox20I TGGCCA 1 cut(s) 3334
MroI TCCGGA 2 cut(s) 505, 3133
MroXI GAANNNNTTC 4 cut(s) 480, 521, 622, 2136
MscI TGGCCA 1 cut(s) 3334
Msp20I TGGCCA 1 cut(s) 3334
MspA1I CMGCKG 2 cut(s) 856, 2558
MspCI CTTAAG 1 cut(s) 1484
MspI CCGG 5 cut(s) 506, 1020, 2874, 3134, 3146
MspR9I CCNGG 4 cut(s) 592, 1020, 2240, 3147
MunI CAATTG 2 cut(s) 257, 1673
Mva1269I GAATGC 2 cut(s) 2575, 3093
MvaI CCWGG 2 cut(s) 592, 2240
MvnI CGCG 2 cut(s) 92, 843
MwoI GCNNNNNNNGC 8 cut(s) 104, 347, 359, 1133, 1217, 1237, 3037, 3290
NciI CCSGG 2 cut(s) 1020, 3147
NcoI CCATGG 1 cut(s) 215
NdeI CATATG 1 cut(s) 1774
NlaIII CATG 7 cut(s) 219, 244, 278, 990, 1396, 1558, 2774
NlaIV GGNNCC 3 cut(s) 2878, 3051, 3263
NmuCI GTSAC 7 cut(s) 205, 1417, 1642, 1726, 2599, 2826, 2869
NruI TCGCGA 1 cut(s) 843
NspV TTCGAA 1 cut(s) 578
PaeR7I CTCGAG 1 cut(s) 2211
PagI TCATGA 4 cut(s) 240, 274, 1392, 2770
PceI AGGCCT 1 cut(s) 1638
PciSI GCTCTTC 3 cut(s) 471, 1614, 1698
PctI GAATGC 2 cut(s) 2575, 3093
PdmI GAANNNNTTC 4 cut(s) 480, 521, 622, 2136
PflMI CCANNNNNTGG 2 cut(s) 987, 2332
PinAI ACCGGT 1 cut(s) 2873
PkrI GCNGC 4 cut(s) 94, 97, 352, 2857
Ple19I CGATCG 2 cut(s) 236, 2629
PleI GAGTC 3 cut(s) 820, 857, 2833
PpsI GAGTC 3 cut(s) 820, 857, 2833
PpuMI RGGWCCY 1 cut(s) 1051
Psp124BI GAGCTC 2 cut(s) 830, 2749
Psp5II RGGWCCY 1 cut(s) 1051
Psp6I CCWGG 2 cut(s) 590, 2238
PspEI GGTNACC 2 cut(s) 262, 2869
PspFI CCCAGC 2 cut(s) 103, 2025
PspGI CCWGG 2 cut(s) 590, 2238
PspN4I GGNNCC 3 cut(s) 2878, 3051, 3263
PspPI GGNCC 8 cut(s) 470, 1051, 1300, 2293, 2719, 3049, 3068, 3262
PspPPI RGGWCCY 1 cut(s) 1051
PspXI VCTCGAGB 1 cut(s) 2211
PstI CTGCAG 1 cut(s) 1807
PstNI CAGNNNCTG 3 cut(s) 1736, 2267, 2603
PsuI RGATCY 3 cut(s) 174, 1441, 1467
PvuI CGATCG 2 cut(s) 236, 2629
PvuII CAGCTG 2 cut(s) 856, 2558
RruI TCGCGA 1 cut(s) 843
SacI GAGCTC 2 cut(s) 830, 2749
SapI GCTCTTC 3 cut(s) 471, 1614, 1698
SatI GCNGC 4 cut(s) 93, 96, 351, 2856
Sau96I GGNCC 8 cut(s) 470, 1051, 1300, 2293, 2719, 3049, 3068, 3262
ScaI AGTACT 1 cut(s) 1846
SchI GAGTC 3 cut(s) 821, 857, 2834
ScrFI CCNGG 4 cut(s) 592, 1020, 2240, 3147
SduI GDGCHC 4 cut(s) 830, 1934, 2550, 2749
SexAI ACCWGGT 1 cut(s) 2238
SfaNI GCATC 5 cut(s) 942, 1004, 1207, 1717, 3027
SfcI CTRYAG 3 cut(s) 1445, 1803, 2679
Sfr274I CTCGAG 1 cut(s) 2211
SfuI TTCGAA 1 cut(s) 578
SinI GGWCC 7 cut(s) 470, 1051, 1300, 2293, 2719, 3068, 3262
SlaI CTCGAG 1 cut(s) 2211
SmlI CTYRAG 4 cut(s) 1484, 1917, 2211, 3301
SmoI CTYRAG 4 cut(s) 1484, 1917, 2211, 3301
SpeI ACTAGT 1 cut(s) 1688
SseBI AGGCCT 1 cut(s) 1638
SsiI CCGC 6 cut(s) 92, 178, 335, 414, 464, 735
SspMI CTAG 4 cut(s) 1191, 1689, 2151, 3095
SstI GAGCTC 2 cut(s) 830, 2749
StuI AGGCCT 1 cut(s) 1638
StyD4I CCNGG 4 cut(s) 590, 1018, 2238, 3145
StyI CCWWGG 3 cut(s) 215, 764, 2001
TaaI ACNGT 7 cut(s) 376, 623, 797, 889, 2127, 2680, 2911
TaiI ACGT 1 cut(s) 1385
TaqII GACCGA 1 cut(s) 1288
TatI WGTACW 8 cut(s) 309, 381, 983, 1002, 1325, 1585, 1844, 2911
TauI GCSGC 1 cut(s) 95
TscAI CASTG 9 cut(s) 361, 802, 1242, 1984, 2079, 2272, 2608, 2662, 3233
TseFI GTSAC 7 cut(s) 205, 1417, 1642, 1726, 2599, 2826, 2869
TseI GCWGC 3 cut(s) 95, 350, 2855
Tsp45I GTSAC 7 cut(s) 205, 1417, 1642, 1726, 2599, 2826, 2869
TspGWI ACGGA 4 cut(s) 440, 444, 474, 1106
TspRI CASTG 9 cut(s) 361, 802, 1242, 1984, 2079, 2272, 2608, 2662, 3233
Van91I CCANNNNNTGG 2 cut(s) 987, 2332
Vha464I CTTAAG 1 cut(s) 1484
VpaK11BI GGWCC 7 cut(s) 470, 1051, 1300, 2293, 2719, 3068, 3262
XapI RAATTY 6 cut(s) 160, 640, 673, 926, 1992, 2707
XcmI CCANNNNNNNNNTGG 1 cut(s) 988
XhoI CTCGAG 1 cut(s) 2211
XmiI GTMKAC 2 cut(s) 1385, 2466
XmnI GAANNNNTTC 4 cut(s) 480, 521, 622, 2136
XspI CTAG 4 cut(s) 1191, 1689, 2151, 3095
ZrmI AGTACT 1 cut(s) 1846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.