RLG00000005657

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0009991
Physical Location & Seq
Forward (+)
648703 .. 654007
5305 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005657

Sequence Viewer

Length: 3423 bp
ATGGTTCGTGTATCGATCCGGCAAACCCAAACCCGACCCGGAAGTCCAACTCCATTCGGTTATCAAACCCAACCCAGTGGAATCGGCGAAGACTGCAGAGTGTGCGTTGCACAGTGCTCGGAGTCTCGGAGAGCGATGCCGACGACAAACCTGGTGCTCCTGGCGTGCTTGGTGTCGCTGGTGGCAGTAATCGCTGCCCTTGATTCAAAATCAGACAGCTCCGGCGAGTGGCACATCCTCACGAAGCGGAACTTCTCTTCTCAGATCCATCTCCATCCTCACATCCTCCTCTTCGTCACTCTTCCATGGTCCGGTGAGTGTCGATCACTCATGAGAGACGTGGCGAGACTGGTCACTGATAGACCTGAAGAGTTCAGCTCGCTGAAGCTGATGGTGTTGCACAGAAATACAGAGAAGATGGTGGCAACTGCGATCGGTGCTGCTTCAGAATGGGAGGAAATTACGGTTTTGTACTATCACCATTCTGTTTCGTACAAATATGGAGGGAGGCTTCGAGCGCAGAACATTTTGAGTTCAATTCGTCCCTATGTGTCGGTTTTAGCTGAAGAACTTCCATTCAAATCGCTGAAGAGTCCGGAAGAGTTGAAAGCTTTCGTTGATTCAACTGACAAAGCTTTGGTTGTGTTTGAGTTTTGTGAATGGACTCCTAAATTGGTGGCCAAGAGGAAGATGAATGGGACTGATCACAGTGGTTTTGGGGAATTTTTTGGATTAAATTTGAATGCAGAGACAAATAGAACCGACTGGTGGAAGAATAACCAGAAGGGTATGGAAACTGCAAAGATGAAGTGTGATGTTAATGCGCTTGGTGCAGTTCCTTGGATTGGGGACTTCAGCTCAGTAAATGATAGTGCTGCTTCTGAGGAGACCGAGAACAGTAGGCCTGATGGTGCATCCTTCTGCACACGTGAAGAATATCAGTTGTTTGACTCTTTCTTCTCTAAGTTTATGACTATTGCAAGAGAGTTCGTTCTGCCTCCTGAAAGGCATAAATTTGGTTTGGTCTCAGAAAGATCTATGCTCTCAGCTCTTGGTATTGGAGATTCCAGTTCGTGGTTGGCAGTGCTATACTTTGCTGGATGCCCTAGTTGTTTGGAGATTATTAAAAAAGAGGATGACCTTAATAATGCTTTAAAGATGGATAGTTCTGTTGTTAAGGAGCTGGAGGGTGATGGCAACACTTTGGAGCCTGCTTTGCCTGCAAACCAGCCATCAGTTCTCTTATTTGTGGATAGATCATCTGACTTGTTAGAGACTAGAATCAATGGCAAGGAAGCTCTTGATGCTTTTCGGGAGCTGGCACTGCACTATCACATGTCTGACCAGATTGGCAGTCAAAGTAGGGACAAGCATGAGAAATCCTCAGTCCAGCATTATCAAGCATTAAGGACCACATCTGGACATCCTAAATTAAAATTATCTCAGGCAGTTCAGATTAGCAAACTAGAAGACAAGATGTCTACTTTCACAATTATAAATGAGGGCAAACAATTCACAGTGGAGAAGTTGGCTTTGGATCTAAAGGGTAATTCCTTGCAGGATATCTTGGAAACTGTACTTAAGGAAAAGAAGAAAGCTAAGTTAAGCTCACTGGTGAAGGATTTGGGTTTCCAACTTTTGTCAGATGATATGGACATCAAACCAGAGAGTACATTGCCATTACCGATTGAATCTGAATCTGATACAGTTACAGAAGAGCCATATAAGGAGGGCCTTGCTACAAGTAGTACTGACTCAGACAGAGATCAACAGCTAGATGCAACAAGAATCTCTACTGAACAGCATCCAGAAACTTCTGAAGTCATGGATACTGAAACGTCATCTCAGAACAATGAAGAAAAAACAGATTATGTTGATACAAGCAATCAGATCTTATCTGTAGATAGTGATCAGCAGCTTGCAGATCATAAATGTGAAGATTTAGATGCAGAAGAAGGTAGTAATTCACAGGGCGAAAAGTCAGCAGAGCAGCAGCTTCACTTTCAAGGCTTTAAGGGTTCATTTTTCTTTTCTGATGGTAATTGTCGGTTACTTCGAGCGCTGACTGGTAGGCCAAAATTTCCAGCCCTTGTAATAGTTGATCCCAATATGCAGCAGCATTATGTCTTCCCAGAGGAGACTAATGTCAATTATTCTTCACTGGTTGATTTCATTTCTGCCTTTCTTAATGGAAGTCTTCTTCCATATCAACAGTCTGAAACTGTACTTGAAAGCTCTAGGAAAGCCACTCAACCACCATTTGTTAATCTGGACTTCCATCAGGTGGATTCTATCCCTCGAGTGACAACTCACACTTTCTCTGAGCTGGTTGTTGGGTTCAATCAATCTGACAGTGATGCTTGGAATAAGGATGTCTTGGTTCTTTTTAGCAATAGATGGTGTGGTTTTTGCCAGCGAATGGAGTTAGTTGTTCATGAAGTATACCGAGCTATGAAGAATTATGCTAAGATGCTGAAAAGGGAATCAAAGAATGAGAAATCAACGTTTCATGATAGCGACGTGAAAAATGAAATGCTGGGGCTGCCATTAATGTACTTGCTGGATTGCACATTGAATGATTGCAGTTTGATACTGAAATCGATGAATCAGAGGGAGGTTTATCCTACTCTGGTGCTCTTTCCAGCAGAAAAGAAGCATGCTTTTCCTTATGAAGGAGATATGGCAGTCACTGAGGTTTTCAAGTTCATGGCTGATCATGGAAGTAACTCTCATCATCTTACCAGTGAAAAAGGAATCTTGTGGACTTTAGCTAAAAAGAGAGGAAGAAATCAAGATTTCTTTATGGTTCAGTCATCTGACGTTCATGAGCAGTCTAGGGATACACTTCATGAATTCCTATTAACAAATGTTCATAAACCAGTAATTGAGGACAAGCAGATGAAGTCTCACATACCCCAAGGCTTGCATGAAGCACCCCCGAATGTGGTGGTTGGTTCTGTCCTAGTTGCAACTGATAAACTTCATAGTGTACACCCCTTCGATAAATCAGAGATTCTCATTGTGAAGGCAGATCAAGTTACTGGATTTCAAGGTTTAATTATCAACAAGCATATTAGATGGGATGCTCTGCCTGAATTGGGAGAAGGAGTTAAGATGTTAGTAAAAGCCCCTTTGTCCTTTGGAGGACCGCTTATAAAGGGTGGAATGCCTCTTGTCGCGTTAACTCAAAAATTTGTCAAGGATGAATATCCGGAAATCTTACCAGGCGTTGCATTTCTTGATCCATCAGCAACAATTCAAAAAATCAAAGAGCTCAAGTTAGGAAATCAATCTGTCACTGACTACTGGTTTTTCTTTGGGTATTCAAGTTGGGGTTGGGACCAGCTCTTTGATGAGATTGATAAAGGAGCTTGGAATTTAAGTGATGATGGTACGAGACATTTAGATTGGCCATCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179 Thioredoxin

Protein Analysis

1141

Amino Acids

128.23

Weight (kDa)

5.41

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 1000 - 1130 9e-11 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1496, 3155
AasI GACNNNNNNGTC 1 cut(s) 42
AccB7I CCANNNNNTGG 3 cut(s) 1074, 2284, 2419
AccI GTMKAC 2 cut(s) 1481, 2442
AccII CGCG 1 cut(s) 3179
AccIII TCCGGA 2 cut(s) 595, 3211
AciI CCGC 2 cut(s) 247, 3149
AclI AACGTT 1 cut(s) 2504
AclWI GGATC 5 cut(s) 10, 259, 1545, 2096, 3236
AcoI YGGCCR 2 cut(s) 678, 3410
AcsI RAATTY 7 cut(s) 722, 736, 1013, 2078, 2852, 3191, 3376
AcuI CTGAAG 7 cut(s) 387, 404, 429, 585, 608, 838, 1839
AcvI CACGTG 1 cut(s) 929
AfaI GTAC 9 cut(s) 473, 494, 1578, 1672, 1750, 2226, 2555, 2991, 3394
AfeI AGCGCT 1 cut(s) 2061
AflII CTTAAG 1 cut(s) 1580
AflIII ACRYGT 2 cut(s) 926, 1335
AhdI GACNNNNNGTC 1 cut(s) 1477
AjiI CACGTC 2 cut(s) 340, 2521
AjnI CCWGG 3 cut(s) 150, 159, 3223
AjuI GAANNNNNNNTTGG 2 cut(s) 1517, 1549
Alw21I GWGCWC 4 cut(s) 119, 159, 2637, 3276
AlwI GGATC 5 cut(s) 10, 259, 1545, 2096, 3236
AlwNI CAGNNNCTG 1 cut(s) 2690
Ama87I CYCGRG 1 cut(s) 2298
Aor13HI TCCGGA 2 cut(s) 595, 3211
Aor51HI AGCGCT 1 cut(s) 2061
AoxI GGCC 5 cut(s) 678, 902, 1732, 2072, 3410
ApeKI GCWGC 9 cut(s) 194, 440, 875, 1915, 1990, 1993, 2113, 2116, 2542
ApoI RAATTY 7 cut(s) 722, 736, 1013, 2078, 2852, 3191, 3376
ArsI GACNNNNNNTTYG 2 cut(s) 3332, 3364
AseI ATTAAT 1 cut(s) 2549
Asp700I GAANNNNTTC 2 cut(s) 570, 611
AspLEI GCGC 3 cut(s) 520, 826, 2062
AspS9I GGNCC 5 cut(s) 309, 1410, 1732, 3146, 3340
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 4 cut(s) 326, 470, 1202, 1627
AvaI CYCGRG 1 cut(s) 2298
AvaII GGWCC 4 cut(s) 309, 1410, 3146, 3340
BalI TGGCCA 2 cut(s) 680, 3412
BanII GRGCYC 1 cut(s) 3276
BbrPI CACGTG 1 cut(s) 929
BbsI GAAGAC 4 cut(s) 96, 1476, 2119, 2189
Bbv12I GWGCWC 4 cut(s) 119, 159, 2637, 3276
BbvI GCAGC 9 cut(s) 181, 427, 862, 1927, 2002, 2005, 2125, 2128, 2529
BcgI CGANNNNNNTGC 2 cut(s) 99, 133
BciT130I CCWGG 3 cut(s) 152, 161, 3225
BciVI GTATCC 2 cut(s) 1822, 2833
BclI TGATCA 3 cut(s) 703, 1909, 2713
BcnI CCSGG 1 cut(s) 39
BfaI CTAG 7 cut(s) 1107, 1278, 1466, 1775, 2238, 2835, 2963
BfmI CTRYAG 2 cut(s) 94, 1899
BfoI RGCGCY 1 cut(s) 2063
BfrI CTTAAG 1 cut(s) 1580
BfuI GTATCC 2 cut(s) 1822, 2833
BglII AGATCT 2 cut(s) 1034, 1890
BisI GCNGC 9 cut(s) 195, 441, 876, 1916, 1991, 1994, 2114, 2117, 2543
BlsI GCNGC 9 cut(s) 196, 442, 877, 1917, 1992, 1995, 2115, 2118, 2544
BmcAI AGTACT 1 cut(s) 1750
Bme1390I CCNGG 4 cut(s) 39, 152, 161, 3225
Bme18I GGWCC 4 cut(s) 309, 1410, 3146, 3340
BmeRI GACNNNNNGTC 1 cut(s) 1477
BmeT110I CYCGRG 1 cut(s) 2298
BmgBI CACGTC 2 cut(s) 340, 2521
BmgT120I GGNCC 5 cut(s) 309, 1410, 1732, 3146, 3340
BmiI GGNNCC 2 cut(s) 1209, 3341
BmrFI CCNGG 4 cut(s) 39, 152, 161, 3225
BmrI ACTGGG 1 cut(s) 69
BmuI ACTGGG 1 cut(s) 69
BoxI GACNNNNGTC 1 cut(s) 2144
BpiI GAAGAC 4 cut(s) 96, 1476, 2119, 2189
BplI GAGNNNNNCTC 4 cut(s) 362, 394, 1367, 1399
BpmI CTGGAG 1 cut(s) 1205
BpuEI CTTGAG 1 cut(s) 3260
BpuMI CCSGG 1 cut(s) 39
Bsa29I ATCGAT 2 cut(s) 14, 2600
BsaAI YACGTR 1 cut(s) 929
BsaBI GATNNNNATC 2 cut(s) 1908, 3207
BsaI GGTCTC 2 cut(s) 881, 1030
BsaJI CCNNGG 3 cut(s) 305, 839, 2917
BsaWI WCCGGW 3 cut(s) 311, 595, 3211
BsaXI ACNNNNNCTCC 2 cut(s) 3093, 3123
Bse3DI GCAATG 1 cut(s) 1673
Bse8I GATNNNNATC 2 cut(s) 1908, 3207
BseAI TCCGGA 2 cut(s) 595, 3211
BseBI CCWGG 3 cut(s) 152, 161, 3225
BseCI ATCGAT 2 cut(s) 14, 2600
BseDI CCNNGG 3 cut(s) 305, 839, 2917
BseJI GATNNNNATC 2 cut(s) 1908, 3207
BseMI GCAATG 1 cut(s) 1673
BseRI GAGGAG 3 cut(s) 278, 899, 2150
BseXI GCAGC 9 cut(s) 181, 427, 862, 1927, 2002, 2005, 2125, 2128, 2529
BseYI CCCAGC 1 cut(s) 2536
BsgI GTGCAG 3 cut(s) 852, 907, 1310
Bsh1236I CGCG 1 cut(s) 3179
Bsh1285I CGRYCG 1 cut(s) 435
BshFI GGCC 5 cut(s) 680, 904, 1734, 2074, 3412
BshVI ATCGAT 2 cut(s) 14, 2600
BsiEI CGRYCG 1 cut(s) 435
BsiHKAI GWGCWC 4 cut(s) 119, 159, 2637, 3276
BsiHKCI CYCGRG 1 cut(s) 2298
BsiSI CCGG 6 cut(s) 19, 39, 222, 312, 596, 3212
BslFI GGGAC 5 cut(s) 528, 712, 863, 1379, 3353
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 5 cut(s) 528, 712, 863, 1379, 3353
BsmI GAATGC 2 cut(s) 748, 3171
BsnI GGCC 5 cut(s) 680, 904, 1734, 2074, 3412
Bso31I GGTCTC 2 cut(s) 881, 1030
BsoBI CYCGRG 1 cut(s) 2298
Bsp1286I GDGCHC 4 cut(s) 119, 159, 2637, 3276
Bsp13I TCCGGA 2 cut(s) 595, 3211
Bsp1407I TGTACA 1 cut(s) 2989
Bsp19I CCATGG 1 cut(s) 305
BspACI CCGC 2 cut(s) 247, 3149
BspANI GGCC 5 cut(s) 680, 904, 1734, 2074, 3412
BspDI ATCGAT 2 cut(s) 14, 2600
BspEI TCCGGA 2 cut(s) 595, 3211
BspFNI CGCG 1 cut(s) 3179
BspHI TCATGA 5 cut(s) 330, 2434, 2509, 2824, 2848
BspLI GGNNCC 2 cut(s) 1209, 3341
BspMAI CTGCAG 1 cut(s) 98
BspPI GGATC 5 cut(s) 10, 259, 1545, 2096, 3236
BspQI GCTCTTC 1 cut(s) 1710
BspTI CTTAAG 1 cut(s) 1580
BspTNI GGTCTC 2 cut(s) 881, 1030
BsrDI GCAATG 1 cut(s) 1673
BsrGI TGTACA 1 cut(s) 2989
BssECI CCNNGG 3 cut(s) 305, 839, 2917
BssNAI GTATAC 1 cut(s) 2443
BssT1I CCWWGG 3 cut(s) 305, 839, 2917
Bst1107I GTATAC 1 cut(s) 2443
Bst2UI CCWGG 3 cut(s) 152, 161, 3225
Bst6I CTCTTC 7 cut(s) 262, 296, 306, 363, 584, 594, 1710
BstAFI CTTAAG 1 cut(s) 1580
BstAPI GCANNNNNTGC 1 cut(s) 102
BstAUI TGTACA 1 cut(s) 2989
BstBAI YACGTR 1 cut(s) 929
BstC8I GCNNGC 9 cut(s) 166, 380, 1212, 1221, 1320, 1920, 2414, 2658, 2924
BstDSI CCRYGG 1 cut(s) 305
BstENI CCTNNNNNAGG 1 cut(s) 2670
BstFNI CGCG 1 cut(s) 3179
BstH2I RGCGCY 1 cut(s) 2063
BstHHI GCGC 3 cut(s) 520, 826, 2062
BstMCI CGRYCG 1 cut(s) 435
BstNI CCWGG 3 cut(s) 152, 161, 3225
BstNSI RCATGY 2 cut(s) 1339, 2660
BstPAI GACNNNNGTC 1 cut(s) 2144
BstSCI CCNGG 4 cut(s) 37, 150, 159, 3223
BstSFI CTRYAG 2 cut(s) 94, 1899
BstUI CGCG 1 cut(s) 3179
BstV1I GCAGC 9 cut(s) 181, 427, 862, 1927, 2002, 2005, 2125, 2128, 2529
BstV2I GAAGAC 4 cut(s) 96, 1476, 2119, 2189
BstX2I RGATCY 4 cut(s) 264, 1034, 1537, 1890
BstXI CCANNNNNNTGG 1 cut(s) 77
BstYI RGATCY 4 cut(s) 264, 1034, 1537, 1890
BstZ17I GTATAC 1 cut(s) 2443
Bsu15I ATCGAT 2 cut(s) 14, 2600
BsuI GTATCC 2 cut(s) 1822, 2833
BsuRI GGCC 5 cut(s) 680, 904, 1734, 2074, 3412
BsuTUI ATCGAT 2 cut(s) 14, 2600
BtgI CCRYGG 1 cut(s) 305
BtgZI GCGATG 1 cut(s) 149
BtrI CACGTC 2 cut(s) 340, 2521
BtsI GCAGTG 2 cut(s) 1089, 1322
Cac8I GCNNGC 9 cut(s) 166, 380, 1212, 1221, 1320, 1920, 2414, 2658, 2924
CaiI CAGNNNCTG 1 cut(s) 2690
CciI TCATGA 5 cut(s) 330, 2434, 2509, 2824, 2848
CfoI GCGC 3 cut(s) 520, 826, 2062
Cfr13I GGNCC 5 cut(s) 309, 1410, 1732, 3146, 3340
ClaI ATCGAT 2 cut(s) 14, 2600
CsiI ACCWGGT 1 cut(s) 150
Csp6I GTAC 9 cut(s) 472, 493, 1577, 1671, 1749, 2225, 2554, 2990, 3393
CviQI GTAC 9 cut(s) 472, 493, 1577, 1671, 1749, 2225, 2554, 2990, 3393
DraI TTTAAA 1 cut(s) 1155
DrdI GACNNNNNNGTC 1 cut(s) 42
DriI GACNNNNNGTC 1 cut(s) 1477
DseDI GACNNNNNNGTC 1 cut(s) 42
EaeI YGGCCR 2 cut(s) 678, 3410
Eam1104I CTCTTC 7 cut(s) 262, 296, 306, 363, 584, 594, 1710
Eam1105I GACNNNNNGTC 1 cut(s) 1477
EarI CTCTTC 7 cut(s) 262, 296, 306, 363, 584, 594, 1710
Ecl136II GAGCTC 1 cut(s) 3274
Eco130I CCWWGG 3 cut(s) 305, 839, 2917
Eco147I AGGCCT 1 cut(s) 904
Eco24I GRGCYC 1 cut(s) 3276
Eco31I GGTCTC 2 cut(s) 881, 1030
Eco32I GATATC 1 cut(s) 1564
Eco47I GGWCC 4 cut(s) 309, 1410, 3146, 3340
Eco47III AGCGCT 1 cut(s) 2061
Eco53kI GAGCTC 1 cut(s) 3274
Eco57I CTGAAG 7 cut(s) 387, 404, 429, 585, 608, 838, 1839
Eco72I CACGTG 1 cut(s) 929
Eco88I CYCGRG 1 cut(s) 2298
EcoICRI GAGCTC 1 cut(s) 3274
EcoNI CCTNNNNNAGG 1 cut(s) 2670
EcoO109I RGGNCCY 1 cut(s) 1732
EcoRI GAATTC 1 cut(s) 2852
EcoRII CCWGG 3 cut(s) 150, 159, 3223
EcoRV GATATC 1 cut(s) 1564
EcoT14I CCWWGG 3 cut(s) 305, 839, 2917
EcoT38I GRGCYC 1 cut(s) 3276
ErhI CCWWGG 3 cut(s) 305, 839, 2917
Esp3I CGTCTC 1 cut(s) 330
FalI AAGNNNNNCTT 4 cut(s) 236, 268, 2360, 2392
FaqI GGGAC 5 cut(s) 528, 712, 863, 1379, 3353
FbaI TGATCA 3 cut(s) 703, 1909, 2713
FblI GTMKAC 2 cut(s) 1481, 2442
Fnu4HI GCNGC 9 cut(s) 195, 441, 876, 1916, 1991, 1994, 2114, 2117, 2543
FriOI GRGCYC 1 cut(s) 3276
Fsp4HI GCNGC 9 cut(s) 195, 441, 876, 1916, 1991, 1994, 2114, 2117, 2543
FspBI CTAG 7 cut(s) 1107, 1278, 1466, 1775, 2238, 2835, 2963
GlaI GCGC 3 cut(s) 519, 825, 2061
GluI GCNGC 9 cut(s) 195, 441, 876, 1916, 1991, 1994, 2114, 2117, 2543
GsaI CCCAGC 1 cut(s) 2540
GsuI CTGGAG 1 cut(s) 1205
HaeII RGCGCY 1 cut(s) 2063
HaeIII GGCC 5 cut(s) 680, 904, 1734, 2074, 3412
HapII CCGG 6 cut(s) 19, 39, 222, 312, 596, 3212
HhaI GCGC 3 cut(s) 520, 826, 2062
Hin6I GCGC 3 cut(s) 518, 824, 2060
HinP1I GCGC 3 cut(s) 518, 824, 2060
HincII GTYRAC 1 cut(s) 3183
HindII GTYRAC 1 cut(s) 3183
HindIII AAGCTT 2 cut(s) 609, 633
HpaI GTTAAC 1 cut(s) 3183
HpaII CCGG 6 cut(s) 19, 39, 222, 312, 596, 3212
HphI GGTGA 4 cut(s) 326, 470, 1202, 1627
Hpy166II GTNNAC 6 cut(s) 1482, 2443, 2763, 2990, 2992, 3183
Hpy8I GTNNAC 6 cut(s) 1482, 2443, 2763, 2990, 2992, 3183
Hpy99I CGWCG 2 cut(s) 145, 2522
HpyAV CCTTC 8 cut(s) 778, 928, 1612, 1949, 2666, 3007, 3019, 3098
HpyCH4IV ACGT 6 cut(s) 339, 928, 1838, 2504, 2520, 2820
HpySE526I ACGT 6 cut(s) 339, 928, 1838, 2504, 2520, 2820
HspAI GCGC 3 cut(s) 518, 824, 2060
Kpn2I TCCGGA 2 cut(s) 595, 3211
Ksp22I TGATCA 3 cut(s) 703, 1909, 2713
KspAI GTTAAC 1 cut(s) 3183
LguI GCTCTTC 1 cut(s) 1710
LmnI GCTCC 6 cut(s) 162, 224, 1180, 1207, 1315, 3368
Lsp1109I GCAGC 9 cut(s) 181, 427, 862, 1927, 2002, 2005, 2125, 2128, 2529
MabI ACCWGGT 1 cut(s) 150
MaeI CTAG 7 cut(s) 1107, 1278, 1466, 1775, 2238, 2835, 2963
MaeII ACGT 6 cut(s) 339, 928, 1838, 2504, 2520, 2820
MaeIII GTNAC 9 cut(s) 295, 352, 1708, 2049, 2302, 2686, 2723, 3037, 3295
MflI RGATCY 4 cut(s) 264, 1034, 1537, 1890
MhlI GDGCHC 4 cut(s) 119, 159, 2637, 3276
MlsI TGGCCA 2 cut(s) 680, 3412
MluNI TGGCCA 2 cut(s) 680, 3412
MlyI GAGTC 5 cut(s) 131, 601, 658, 944, 1748
MmeI TCCRAC 2 cut(s) 71, 1657
Mox20I TGGCCA 2 cut(s) 680, 3412
MroI TCCGGA 2 cut(s) 595, 3211
MroXI GAANNNNTTC 2 cut(s) 570, 611
MscI TGGCCA 2 cut(s) 680, 3412
MslI CAYNNNNRTG 1 cut(s) 1932
Msp20I TGGCCA 2 cut(s) 680, 3412
MspCI CTTAAG 1 cut(s) 1580
MspI CCGG 6 cut(s) 19, 39, 222, 312, 596, 3212
MspR9I CCNGG 4 cut(s) 39, 152, 161, 3225
Mva1269I GAATGC 2 cut(s) 748, 3171
MvaI CCWGG 3 cut(s) 152, 161, 3225
MvnI CGCG 1 cut(s) 3179
NciI CCSGG 1 cut(s) 39
NcoI CCATGG 1 cut(s) 305
NlaIV GGNNCC 2 cut(s) 1209, 3341
NmuCI GTSAC 5 cut(s) 295, 352, 2302, 2686, 3295
NspI RCATGY 2 cut(s) 1339, 2660
PaeI GCATGC 1 cut(s) 2660
PaeR7I CTCGAG 1 cut(s) 2298
PagI TCATGA 5 cut(s) 330, 2434, 2509, 2824, 2848
PceI AGGCCT 1 cut(s) 904
PciI ACATGT 1 cut(s) 1335
PciSI GCTCTTC 1 cut(s) 1710
PcsI WCGNNNNNNNCGW 1 cut(s) 2053
PctI GAATGC 2 cut(s) 748, 3171
PdmI GAANNNNTTC 2 cut(s) 570, 611
PflMI CCANNNNNTGG 3 cut(s) 1074, 2284, 2419
PkrI GCNGC 9 cut(s) 196, 442, 877, 1917, 1992, 1995, 2115, 2118, 2544
Ple19I CGATCG 1 cut(s) 435
PleI GAGTC 5 cut(s) 130, 600, 658, 944, 1748
PmaCI CACGTG 1 cut(s) 929
PmlI CACGTG 1 cut(s) 929
PpsI GAGTC 5 cut(s) 130, 600, 658, 944, 1748
Ppu21I YACGTR 1 cut(s) 929
PscI ACATGT 1 cut(s) 1335
PshAI GACNNNNGTC 1 cut(s) 2144
PshBI ATTAAT 1 cut(s) 2549
PsiI TTATAA 2 cut(s) 1496, 3155
Psp124BI GAGCTC 1 cut(s) 3276
Psp1406I AACGTT 1 cut(s) 2504
Psp6I CCWGG 3 cut(s) 150, 159, 3223
PspCI CACGTG 1 cut(s) 929
PspFI CCCAGC 1 cut(s) 2536
PspGI CCWGG 3 cut(s) 150, 159, 3223
PspN4I GGNNCC 2 cut(s) 1209, 3341
PspPI GGNCC 5 cut(s) 309, 1410, 1732, 3146, 3340
PspXI VCTCGAGB 1 cut(s) 2298
PstI CTGCAG 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 2690
PsuI RGATCY 4 cut(s) 264, 1034, 1537, 1890
PvuI CGATCG 1 cut(s) 435
RsaI GTAC 9 cut(s) 473, 494, 1578, 1672, 1750, 2226, 2555, 2991, 3394
RsaNI GTAC 9 cut(s) 472, 493, 1577, 1671, 1749, 2225, 2554, 2990, 3393
RseI CAYNNNNRTG 1 cut(s) 1932
SacI GAGCTC 1 cut(s) 3276
SapI GCTCTTC 1 cut(s) 1710
SatI GCNGC 9 cut(s) 195, 441, 876, 1916, 1991, 1994, 2114, 2117, 2543
Sau96I GGNCC 5 cut(s) 309, 1410, 1732, 3146, 3340
ScaI AGTACT 1 cut(s) 1750
SchI GAGTC 5 cut(s) 131, 601, 658, 944, 1748
ScrFI CCNGG 4 cut(s) 39, 152, 161, 3225
SduI GDGCHC 4 cut(s) 119, 159, 2637, 3276
SexAI ACCWGGT 1 cut(s) 150
SfcI CTRYAG 2 cut(s) 94, 1899
Sfr274I CTCGAG 1 cut(s) 2298
SinI GGWCC 4 cut(s) 309, 1410, 3146, 3340
SlaI CTCGAG 1 cut(s) 2298
SmiMI CAYNNNNRTG 1 cut(s) 1932
SmlI CTYRAG 3 cut(s) 1580, 2298, 3275
SmoI CTYRAG 3 cut(s) 1580, 2298, 3275
SphI GCATGC 1 cut(s) 2660
SseBI AGGCCT 1 cut(s) 904
SsiI CCGC 2 cut(s) 247, 3149
SspMI CTAG 7 cut(s) 1107, 1278, 1466, 1775, 2238, 2835, 2963
SstI GAGCTC 1 cut(s) 3276
StuI AGGCCT 1 cut(s) 904
StyD4I CCNGG 4 cut(s) 37, 150, 159, 3223
StyI CCWWGG 3 cut(s) 305, 839, 2917
TaiI ACGT 6 cut(s) 342, 931, 1841, 2507, 2523, 2823
TaqI TCGA 7 cut(s) 14, 322, 514, 2056, 2299, 2600, 3000
TaqII GACCGA 1 cut(s) 905
TatI WGTACW 7 cut(s) 471, 1576, 1670, 1748, 2224, 2553, 2989
TseFI GTSAC 5 cut(s) 295, 352, 2302, 2686, 3295
TseI GCWGC 9 cut(s) 194, 440, 875, 1915, 1990, 1993, 2113, 2116, 2542
Tsp45I GTSAC 5 cut(s) 295, 352, 2302, 2686, 3295
Van91I CCANNNNNTGG 3 cut(s) 1074, 2284, 2419
Vha464I CTTAAG 1 cut(s) 1580
VpaK11BI GGWCC 4 cut(s) 309, 1410, 3146, 3340
VspI ATTAAT 1 cut(s) 2549
XagI CCTNNNNNAGG 1 cut(s) 2670
XapI RAATTY 7 cut(s) 722, 736, 1013, 2078, 2852, 3191, 3376
XceI RCATGY 2 cut(s) 1339, 2660
XcmI CCANNNNNNNNNTGG 1 cut(s) 1075
XhoI CTCGAG 1 cut(s) 2298
XmiI GTMKAC 2 cut(s) 1481, 2442
XmnI GAANNNNTTC 2 cut(s) 570, 611
XspI CTAG 7 cut(s) 1107, 1278, 1466, 1775, 2238, 2835, 2963
ZrmI AGTACT 1 cut(s) 1750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.