FvH4_5g00830

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016839
Physical Location & Seq
Forward (+)
471454 .. 472129
676 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00830.t1

Sequence Viewer

Length: 555 bp
ATGATCCATCCCCGGTCACAGTTTTACGGGCTAAACATGTGTCCTCTGGCCGTTCCCGAGAGGACACTCCAGACCTTCCCGCCGGAGAGGCACACCAACCTTGTACGCCGACCGGGAGGCCTCATCTCCCTCCCTGAGGGATGGAAGGCTCCGGAGACCCGCCCGGGAAACTCACAGTACCGTTTGACGAAGCTGCTTCTGAATGTGACGATAGAGAGAAGCCTCGGGCCTGTGCACGTTGTGATGTCGTCGGAGAATACGGTCAGTGATTTGATCAAGTCCGTGGTAGATATTTACGTGAAGGAGAAGAGGAGGCCTTTGGTGAAGGAGACTGATCCGCACCGTTTCAAGCTTCACTACTCACAGTTCAGTCTTGAGAGTATAAAGGCAGATGAGAAGTTGATACAGTTGGGATCTAGAAATTTTTTCTTGTGCTCAAAGTCGTCAGATTCAGCTACCGTTTTATCCAGCTCTGTCAGCAATTGCTCAAAGGAAGCAGAGAAGGACATCAATTCCCCATTTCCATTGATAAAATTTATGGATTTCTTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.89

Weight (kDa)

9.32

Isoelectric Point (pI)

55.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 62 - 145 3.6e-37 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016839)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00830
malus_domestica MD04G1077900.v1.1
prunus_persica Prupe.5G080800_v2.0.a1
pyrus_communis pycom04g06980
rosa_chinensis RchiOBHm_Chr7g0201091
rosa_laevigata RLG00000003692
rosa_multiflora Rmu_sc0000785.1_g000016
rosa_roxburghii Rroxscaffold_3G00255520
rosa_samantha Rh7AG187900 Rh7BG191400 Rh7CG198800 Rh7DG193600
rosa_wichuraiana Rw7G016500 Rw7G016570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 3 cut(s) 80, 160, 338
AclWI GGATC 2 cut(s) 329, 421
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 2 cut(s) 421, 533
AdeI CACNNNGTG 1 cut(s) 241
AfaI GTAC 2 cut(s) 105, 179
AfiI CCNNNNNNNGG 1 cut(s) 136
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 1 cut(s) 349
AluBI AGCT 4 cut(s) 193, 352, 455, 471
AluI AGCT 4 cut(s) 193, 352, 455, 471
Alw21I GWGCWC 2 cut(s) 237, 437
Alw26I GTCTC 2 cut(s) 149, 323
Alw44I GTGCAC 1 cut(s) 233
AlwI GGATC 2 cut(s) 329, 421
Ama87I CYCGRG 3 cut(s) 56, 163, 224
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 4 cut(s) 48, 118, 227, 314
ApaLI GTGCAC 1 cut(s) 233
ApeKI GCWGC 1 cut(s) 193
ApoI RAATTY 2 cut(s) 421, 533
AspS9I GGNCC 1 cut(s) 227
AsuC2I CCSGG 4 cut(s) 13, 114, 164, 165
AsuHPI GGTGA 1 cut(s) 334
AvaI CYCGRG 3 cut(s) 56, 163, 224
AxyI CCTNAGG 1 cut(s) 135
BaeGI GKGCMC 1 cut(s) 237
Bbv12I GWGCWC 2 cut(s) 237, 437
BbvI GCAGC 1 cut(s) 180
BccI CCATC 2 cut(s) 15, 135
BceAI ACGGC 1 cut(s) 35
BclI TGATCA 1 cut(s) 273
BcnI CCSGG 4 cut(s) 13, 114, 164, 165
BcoDI GTCTC 2 cut(s) 149, 323
BfaI CTAG 1 cut(s) 417
BglI GCCNNNNNGGC 1 cut(s) 88
BisI GCNGC 1 cut(s) 194
BlsI GCNGC 1 cut(s) 195
Bme1390I CCNGG 4 cut(s) 13, 114, 164, 165
BmeT110I CYCGRG 3 cut(s) 56, 163, 224
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 4 cut(s) 13, 114, 164, 165
BpmI CTGGAG 1 cut(s) 53
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 4 cut(s) 13, 114, 164, 165
BsaAI YACGTR 1 cut(s) 298
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 4 cut(s) 11, 163, 223, 282
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 1 cut(s) 136
Bse21I CCTNAGG 1 cut(s) 135
BseAI TCCGGA 1 cut(s) 151
BseDI CCNNGG 4 cut(s) 11, 163, 223, 282
BseGI GGATG 2 cut(s) 7, 146
BseLI CCNNNNNNNGG 1 cut(s) 136
BseMII CTCAG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 325
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 1 cut(s) 180
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 4 cut(s) 50, 120, 229, 316
BsiEI CGRYCG 1 cut(s) 113
BsiHKAI GWGCWC 2 cut(s) 237, 437
BsiHKCI CYCGRG 3 cut(s) 56, 163, 224
BsiSI CCGG 5 cut(s) 13, 83, 113, 152, 164
BslI CCNNNNNNNGG 1 cut(s) 136
BsmAI GTCTC 2 cut(s) 149, 323
BsnI GGCC 4 cut(s) 50, 120, 229, 316
Bso31I GGTCTC 1 cut(s) 149
BsoBI CYCGRG 3 cut(s) 56, 163, 224
Bsp1286I GDGCHC 2 cut(s) 237, 437
Bsp13I TCCGGA 1 cut(s) 151
Bsp143I GATC 4 cut(s) 3, 273, 334, 413
BspACI CCGC 3 cut(s) 80, 160, 338
BspANI GGCC 4 cut(s) 50, 120, 229, 316
BspCNI CTCAG 1 cut(s) 127
BspEI TCCGGA 1 cut(s) 151
BspLI GGNNCC 1 cut(s) 150
BspPI GGATC 2 cut(s) 329, 421
BspTNI GGTCTC 1 cut(s) 149
BssECI CCNNGG 4 cut(s) 11, 163, 223, 282
BssMI GATC 4 cut(s) 3, 273, 334, 413
Bst4CI ACNGT 8 cut(s) 21, 177, 182, 262, 344, 366, 408, 460
Bst6I CTCTTC 1 cut(s) 302
BstBAI YACGTR 1 cut(s) 298
BstDEI CTNAG 1 cut(s) 135
BstDSI CCRYGG 1 cut(s) 282
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 2 cut(s) 7, 146
BstKTI GATC 4 cut(s) 6, 276, 337, 416
BstMAI GTCTC 2 cut(s) 149, 323
BstMBI GATC 4 cut(s) 3, 273, 334, 413
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 2 cut(s) 88, 477
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 4 cut(s) 11, 112, 162, 163
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
Bsu36I CCTNAGG 1 cut(s) 135
BsuRI GGCC 4 cut(s) 50, 120, 229, 316
BtgI CCRYGG 1 cut(s) 282
BtsCI GGATG 2 cut(s) 7, 146
BtsIMutI CAGTG 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 227
Cfr9I CCCGGG 1 cut(s) 163
Csp6I GTAC 2 cut(s) 104, 178
CviAII CATG 1 cut(s) 37
CviQI GTAC 2 cut(s) 104, 178
DdeI CTNAG 1 cut(s) 135
DpnI GATC 4 cut(s) 5, 275, 336, 415
DpnII GATC 4 cut(s) 3, 273, 334, 413
DraIII CACNNNGTG 1 cut(s) 241
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 302
EarI CTCTTC 1 cut(s) 302
Eco147I AGGCCT 2 cut(s) 120, 316
Eco31I GGTCTC 1 cut(s) 149
Eco81I CCTNAGG 1 cut(s) 135
Eco88I CYCGRG 3 cut(s) 56, 163, 224
EcoNI CCTNNNNNAGG 1 cut(s) 134
FaeI CATG 1 cut(s) 40
FaiI YATR 3 cut(s) 38, 383, 539
FatI CATG 1 cut(s) 36
FauI CCCGC 2 cut(s) 87, 167
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 194
FokI GGATG 1 cut(s) 153
Fsp4HI GCNGC 1 cut(s) 194
FspBI CTAG 1 cut(s) 417
GluI GCNGC 1 cut(s) 194
GsuI CTGGAG 1 cut(s) 53
HaeIII GGCC 4 cut(s) 50, 120, 229, 316
HapII CCGG 5 cut(s) 13, 83, 113, 152, 164
Hin1II CATG 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 1 cut(s) 449
HpaII CCGG 5 cut(s) 13, 83, 113, 152, 164
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 3 cut(s) 201, 253, 448
Hpy188III TCNNGA 5 cut(s) 56, 70, 152, 374, 417
Hpy8I GTNNAC 1 cut(s) 235
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 5 cut(s) 85, 139, 295, 319, 496
HpyCH4III ACNGT 8 cut(s) 21, 177, 182, 262, 344, 366, 408, 460
HpyCH4IV ACGT 2 cut(s) 237, 297
HpyCH4V TGCA 1 cut(s) 235
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 477
HpyF3I CTNAG 1 cut(s) 135
HpySE526I ACGT 2 cut(s) 237, 297
Hsp92II CATG 1 cut(s) 40
Kpn2I TCCGGA 1 cut(s) 151
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 4 cut(s) 3, 273, 334, 413
LmnI GCTCC 1 cut(s) 154
Lsp1109I GCAGC 1 cut(s) 180
MaeI CTAG 1 cut(s) 417
MaeII ACGT 2 cut(s) 237, 297
MaeIII GTNAC 2 cut(s) 15, 205
MalI GATC 4 cut(s) 5, 275, 336, 415
MboI GATC 4 cut(s) 3, 273, 334, 413
MboII GAAGA 1 cut(s) 319
MfeI CAATTG 1 cut(s) 481
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 2 cut(s) 237, 437
MluCI AATT 4 cut(s) 421, 481, 511, 533
MmeI TCCRAC 1 cut(s) 231
MroI TCCGGA 1 cut(s) 151
MspI CCGG 5 cut(s) 13, 83, 113, 152, 164
MspR9I CCNGG 4 cut(s) 13, 114, 164, 165
MunI CAATTG 1 cut(s) 481
MwoI GCNNNNNNNGC 2 cut(s) 88, 477
NciI CCSGG 4 cut(s) 13, 114, 164, 165
NdeII GATC 4 cut(s) 3, 273, 334, 413
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 1 cut(s) 150
NmuCI GTSAC 2 cut(s) 15, 205
NspI RCATGY 1 cut(s) 40
PceI AGGCCT 2 cut(s) 120, 316
PciI ACATGT 1 cut(s) 36
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 1 cut(s) 195
Ppu21I YACGTR 1 cut(s) 298
PscI ACATGT 1 cut(s) 36
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 1 cut(s) 227
PsuI RGATCY 1 cut(s) 413
RsaI GTAC 2 cut(s) 105, 179
RsaNI GTAC 2 cut(s) 104, 178
SatI GCNGC 1 cut(s) 194
Sau3AI GATC 4 cut(s) 3, 273, 334, 413
Sau96I GGNCC 1 cut(s) 227
ScrFI CCNGG 4 cut(s) 13, 114, 164, 165
SduI GDGCHC 2 cut(s) 237, 437
SetI ASST 8 cut(s) 77, 102, 195, 240, 300, 354, 457, 473
SmaI CCCGGG 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 4 cut(s) 421, 481, 511, 533
SseBI AGGCCT 2 cut(s) 120, 316
SsiI CCGC 3 cut(s) 80, 160, 338
SspMI CTAG 1 cut(s) 417
StuI AGGCCT 2 cut(s) 120, 316
StyD4I CCNGG 4 cut(s) 11, 112, 162, 163
TaaI ACNGT 8 cut(s) 21, 177, 182, 262, 344, 366, 408, 460
TaiI ACGT 2 cut(s) 240, 300
TasI AATT 4 cut(s) 421, 481, 511, 533
TfiI GAWTC 1 cut(s) 449
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 2 cut(s) 15, 205
TseI GCWGC 1 cut(s) 193
Tsp45I GTSAC 2 cut(s) 15, 205
TspGWI ACGGA 1 cut(s) 271
TspMI CCCGGG 1 cut(s) 163
TspRI CASTG 1 cut(s) 271
VneI GTGCAC 1 cut(s) 233
XagI CCTNNNNNAGG 1 cut(s) 134
XapI RAATTY 2 cut(s) 421, 533
XbaI TCTAGA 1 cut(s) 416
XceI RCATGY 1 cut(s) 40
XmaI CCCGGG 1 cut(s) 163
XspI CTAG 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.