Rw7G016570

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0016839
Physical Location & Seq
Reverse (-)
16959928 .. 16966686
6759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016570.1

Sequence Viewer

Length: 546 bp
ATGATTCATCCGCGGTCACAGTTTCCGGCCATTTCCGAGAGGACCCTTCAGACCTTCCCGCCGGAGAGGCACACCAACCTCGTACGCCGACCGGGAGGTCTCATCTCCGTCCCCGGAAGGCTCTGGAAGGCTCAGGAGACCCGCCTGGAGAACTCCCAGTACCGTTTGACGAAGCTGCTTCTGAATGTGACGCTAGAGAGGAGCTTGGGGCCTGTACACGTGTTGATGTCGCCGGAGAACACGGTCAGTGATTTGATAAAGTCTGCCGTGGAAATATACGTGAAGGAGAAGAGAAGGCCTTTGGTGAAGGAGACTAATCCGCACCGTTTCGAGCTTCACTACTCACAATTCAGTCTAGAGAGTTTGAAGGAAGATCAGAAGTTGATAAAGTTGGGGTCTAGAAATTTTTTCTTGTGCTCAAAGGCATCAGATTCAGATGCCCTTTTATCTAGCTCTGTCGTCAATCCTTCGAATTGCTCAAAGGAAGCAAAGAAGGAAATCAATTCTCCATTTCCATTAATAAAGTTAATGGATTTCTTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.71

Weight (kDa)

9.66

Isoelectric Point (pI)

55.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 56 - 139 3.1e-37 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016839)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00830
malus_domestica MD04G1077900.v1.1
prunus_persica Prupe.5G080800_v2.0.a1
pyrus_communis pycom04g06980
rosa_chinensis RchiOBHm_Chr7g0201091
rosa_laevigata RLG00000003692
rosa_multiflora Rmu_sc0000785.1_g000016
rosa_roxburghii Rroxscaffold_3G00255520
rosa_samantha Rh7AG187900 Rh7BG191400 Rh7CG198800 Rh7DG193600
rosa_wichuraiana Rw7G016500 Rw7G016570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 96
AccII CGCG 1 cut(s) 13
AciI CCGC 5 cut(s) 11, 13, 59, 142, 320
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 1 cut(s) 403
AcuI CTGAAG 1 cut(s) 32
AcvI CACGTG 1 cut(s) 220
AfaI GTAC 3 cut(s) 84, 161, 216
AflIII ACRYGT 2 cut(s) 217, 219
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 4 cut(s) 175, 204, 334, 453
AluI AGCT 4 cut(s) 175, 204, 334, 453
Alw21I GWGCWC 1 cut(s) 419
Alw26I GTCTC 3 cut(s) 104, 131, 305
AoxI GGCC 3 cut(s) 27, 209, 296
ApeKI GCWGC 1 cut(s) 175
ApoI RAATTY 1 cut(s) 403
AseI ATTAAT 1 cut(s) 518
AspS9I GGNCC 2 cut(s) 42, 209
AsuC2I CCSGG 2 cut(s) 93, 114
AsuHPI GGTGA 1 cut(s) 316
AsuII TTCGAA 1 cut(s) 470
AvaII GGWCC 1 cut(s) 42
BbrPI CACGTG 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 419
BbvI GCAGC 1 cut(s) 162
BceAI ACGGC 1 cut(s) 251
BciT130I CCWGG 1 cut(s) 146
BcnI CCSGG 2 cut(s) 93, 114
BcoDI GTCTC 3 cut(s) 104, 131, 305
BfaI CTAG 4 cut(s) 194, 356, 399, 450
BglI GCCNNNNNGGC 1 cut(s) 67
BisI GCNGC 1 cut(s) 176
BlsI GCNGC 1 cut(s) 177
Bme1390I CCNGG 3 cut(s) 93, 114, 146
Bme18I GGWCC 1 cut(s) 42
BmgT120I GGNCC 2 cut(s) 42, 209
BmiI GGNNCC 2 cut(s) 44, 210
BmrFI CCNGG 3 cut(s) 93, 114, 146
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 2 cut(s) 427, 434
BmuI ACTGGG 1 cut(s) 151
BpmI CTGGAG 1 cut(s) 167
Bpu10I CCTNAGC 1 cut(s) 132
Bpu14I TTCGAA 1 cut(s) 470
BpuMI CCSGG 2 cut(s) 93, 114
BsaAI YACGTR 2 cut(s) 220, 280
BsaI GGTCTC 2 cut(s) 104, 131
BsaJI CCNNGG 3 cut(s) 11, 112, 267
Bse1I ACTGG 1 cut(s) 157
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 3 cut(s) 11, 112, 267
BseGI GGATG 1 cut(s) 7
BseMII CTCAG 1 cut(s) 146
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 214
BseXI GCAGC 1 cut(s) 162
Bsh1236I CGCG 1 cut(s) 13
Bsh1285I CGRYCG 1 cut(s) 92
BshFI GGCC 3 cut(s) 29, 211, 298
BsiEI CGRYCG 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 419
BsiSI CCGG 5 cut(s) 26, 62, 92, 114, 233
BsiWI CGTACG 1 cut(s) 82
BslFI GGGAC 1 cut(s) 95
BsmAI GTCTC 3 cut(s) 104, 131, 305
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 3 cut(s) 29, 211, 298
Bso31I GGTCTC 2 cut(s) 104, 131
Bsp119I TTCGAA 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 419
Bsp1407I TGTACA 1 cut(s) 214
Bsp143I GATC 1 cut(s) 373
BspACI CCGC 5 cut(s) 11, 13, 59, 142, 320
BspANI GGCC 3 cut(s) 29, 211, 298
BspCNI CTCAG 1 cut(s) 145
BspFNI CGCG 1 cut(s) 13
BspLI GGNNCC 2 cut(s) 44, 210
BspT104I TTCGAA 1 cut(s) 470
BspTNI GGTCTC 2 cut(s) 104, 131
BsrGI TGTACA 1 cut(s) 214
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 3 cut(s) 11, 112, 267
BssMI GATC 1 cut(s) 373
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 4 cut(s) 21, 164, 244, 326
Bst6I CTCTTC 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 214
BstBAI YACGTR 2 cut(s) 220, 280
BstBI TTCGAA 1 cut(s) 470
BstDEI CTNAG 1 cut(s) 132
BstDSI CCRYGG 2 cut(s) 11, 267
BstF5I GGATG 1 cut(s) 7
BstFNI CGCG 1 cut(s) 13
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 3 cut(s) 104, 131, 305
BstMBI GATC 1 cut(s) 373
BstMCI CGRYCG 1 cut(s) 92
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 3 cut(s) 91, 112, 144
BstUI CGCG 1 cut(s) 13
BstV1I GCAGC 1 cut(s) 162
BsuRI GGCC 3 cut(s) 29, 211, 298
BtgI CCRYGG 2 cut(s) 11, 267
BtsCI GGATG 1 cut(s) 7
BtsIMutI CAGTG 1 cut(s) 253
Cfr13I GGNCC 2 cut(s) 42, 209
Cfr42I CCGCGG 1 cut(s) 14
CseI GACGC 1 cut(s) 199
Csp6I GTAC 3 cut(s) 83, 160, 215
CviJI RGCY 9 cut(s) 29, 121, 131, 175, 204, 211, 298, 334, 453
CviKI_1 RGCY 9 cut(s) 29, 121, 131, 175, 204, 211, 298, 334, 453
CviQI GTAC 3 cut(s) 83, 160, 215
DdeI CTNAG 1 cut(s) 132
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
DrdI GACNNNNNNGTC 1 cut(s) 96
DseDI GACNNNNNNGTC 1 cut(s) 96
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Eco147I AGGCCT 1 cut(s) 298
Eco31I GGTCTC 2 cut(s) 104, 131
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 32
Eco72I CACGTG 1 cut(s) 220
EcoO109I RGGNCCY 2 cut(s) 42, 209
EcoRII CCWGG 1 cut(s) 144
FaiI YATR 1 cut(s) 277
FaqI GGGAC 1 cut(s) 95
FauI CCCGC 2 cut(s) 66, 149
Fnu4HI GCNGC 1 cut(s) 176
Fsp4HI GCNGC 1 cut(s) 176
FspBI CTAG 4 cut(s) 194, 356, 399, 450
GluI GCNGC 1 cut(s) 176
GsuI CTGGAG 1 cut(s) 167
HaeIII GGCC 3 cut(s) 29, 211, 298
HapII CCGG 5 cut(s) 26, 62, 92, 114, 233
HgaI GACGC 1 cut(s) 199
HinfI GANTC 2 cut(s) 4, 431
HpaII CCGG 5 cut(s) 26, 62, 92, 114, 233
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 6 cut(s) 37, 51, 183, 378, 430, 436
Hpy188III TCNNGA 4 cut(s) 124, 134, 356, 399
Hpy8I GTNNAC 1 cut(s) 217
HpyCH4III ACNGT 4 cut(s) 21, 164, 244, 326
HpyCH4IV ACGT 2 cut(s) 219, 279
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
HpyF3I CTNAG 1 cut(s) 132
HpySE526I ACGT 2 cut(s) 219, 279
KspI CCGCGG 1 cut(s) 14
Kzo9I GATC 1 cut(s) 373
LmnI GCTCC 1 cut(s) 201
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 2 cut(s) 427, 434
MaeI CTAG 4 cut(s) 194, 356, 399, 450
MaeII ACGT 2 cut(s) 219, 279
MaeIII GTNAC 2 cut(s) 15, 187
MalI GATC 1 cut(s) 375
MboI GATC 1 cut(s) 373
MboII GAAGA 2 cut(s) 301, 383
MhlI GDGCHC 1 cut(s) 419
MluCI AATT 4 cut(s) 347, 403, 472, 502
MnlI CCTC 5 cut(s) 33, 60, 89, 89, 192
MseI TTAA 2 cut(s) 518, 527
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 5 cut(s) 26, 62, 92, 114, 233
MspR9I CCNGG 3 cut(s) 93, 114, 146
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 1 cut(s) 67
NciI CCSGG 2 cut(s) 93, 114
NdeII GATC 1 cut(s) 373
NlaIV GGNNCC 2 cut(s) 44, 210
NmuCI GTSAC 2 cut(s) 15, 187
NspV TTCGAA 1 cut(s) 470
PceI AGGCCT 1 cut(s) 298
PfeI GAWTC 2 cut(s) 4, 431
Pfl23II CGTACG 1 cut(s) 82
PkrI GCNGC 1 cut(s) 177
PmaCI CACGTG 1 cut(s) 220
PmlI CACGTG 1 cut(s) 220
Ppu21I YACGTR 2 cut(s) 220, 280
PpuMI RGGWCCY 1 cut(s) 42
PshBI ATTAAT 1 cut(s) 518
Psp5II RGGWCCY 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 144
PspCI CACGTG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 144
PspLI CGTACG 1 cut(s) 82
PspN4I GGNNCC 2 cut(s) 44, 210
PspPI GGNCC 2 cut(s) 42, 209
PspPPI RGGWCCY 1 cut(s) 42
PsrI GAACNNNNNNTAC 2 cut(s) 143, 175
RsaI GTAC 3 cut(s) 84, 161, 216
RsaNI GTAC 3 cut(s) 83, 160, 215
SacII CCGCGG 1 cut(s) 14
SaqAI TTAA 2 cut(s) 518, 527
SatI GCNGC 1 cut(s) 176
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 2 cut(s) 42, 209
ScrFI CCNGG 3 cut(s) 93, 114, 146
SduI GDGCHC 1 cut(s) 419
SetI ASST 9 cut(s) 56, 81, 100, 177, 206, 222, 282, 336, 455
SfaNI GCATC 2 cut(s) 427, 434
Sfr303I CCGCGG 1 cut(s) 14
SfuI TTCGAA 1 cut(s) 470
SgrBI CCGCGG 1 cut(s) 14
SinI GGWCC 1 cut(s) 42
Sse9I AATT 4 cut(s) 347, 403, 472, 502
SseBI AGGCCT 1 cut(s) 298
SsiI CCGC 5 cut(s) 11, 13, 59, 142, 320
SspMI CTAG 4 cut(s) 194, 356, 399, 450
StuI AGGCCT 1 cut(s) 298
StyD4I CCNGG 3 cut(s) 91, 112, 144
TaaI ACNGT 4 cut(s) 21, 164, 244, 326
TaiI ACGT 2 cut(s) 222, 282
TaqI TCGA 2 cut(s) 330, 470
TasI AATT 4 cut(s) 347, 403, 472, 502
TatI WGTACW 1 cut(s) 214
TfiI GAWTC 2 cut(s) 4, 431
Tru1I TTAA 2 cut(s) 518, 527
Tru9I TTAA 2 cut(s) 518, 527
TscAI CASTG 1 cut(s) 253
TseFI GTSAC 2 cut(s) 15, 187
TseI GCWGC 1 cut(s) 175
Tsp45I GTSAC 2 cut(s) 15, 187
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 1 cut(s) 253
VpaK11BI GGWCC 1 cut(s) 42
VspI ATTAAT 1 cut(s) 518
XapI RAATTY 1 cut(s) 403
XbaI TCTAGA 2 cut(s) 355, 398
XspI CTAG 4 cut(s) 194, 356, 399, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.