Prupe.5G080800_v2.0.a1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0016839
Physical Location & Seq
Forward (+)
9438317 .. 9440007
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G080800.1

Sequence Viewer

Length: 657 bp
ATGACAAAACCCCAACTCTCATCACTCTCACTCTCATTTTCTCACACAAGCAATTCCACAAACACCTTCCCTACACTCTTCATTCTCGCGTTCTCGCAACCACAAACCATGATTCAACGATCACACCATTACCGGCGAAACCACCTCCACGCTGTTCCTCCGGCGACCTCCGGGAGGGCTCTAGCGCTTCATACGTTTCCTCCGGAGAGTGGCACCGGAGAGCTCGTTCGCCGGCGCGGCGGCCTCATCTCCGTCCCCGTGAAGCTTTGGTCGCCGCCGGAGACCCACCGCCGGATGACGAAGCTCCTTCTAAACGTGACGATCGAGAGGAGCTTAGGACCTGTGCAGGTGGTAATGTCGCAGGAGAACACCGTCAGCGATTTGATCAAGGCGGCCGTAGAGATTTACGTGAGGGAGAAGAGAAGGCCTTTGGTGAAGGAGACCGATCCGCACCGTTTCGAGCTTCACTACTCGCAGTTCAGCTTGGAGAGTTTGAAGGGAGATGAGAAGTTGATAAAGTTGGGCTCTAGGAATTTCTTTTTGTGCAGCTCAAAGTCAAAGCCCTCTGCCAATCATTCTAATTACTGCGGGGAGGCAAAGAAGGAAACCAGGAATTCTCCATTTCCTTGGGCAAAGTTTATGGATTTCTTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

24.77

Weight (kDa)

10.09

Isoelectric Point (pI)

59.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016839)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00830
malus_domestica MD04G1077900.v1.1
prunus_persica Prupe.5G080800_v2.0.a1
pyrus_communis pycom04g06980
rosa_chinensis RchiOBHm_Chr7g0201091
rosa_laevigata RLG00000003692
rosa_multiflora Rmu_sc0000785.1_g000016
rosa_roxburghii Rroxscaffold_3G00255520
rosa_samantha Rh7AG187900 Rh7BG191400 Rh7CG198800 Rh7DG193600
rosa_wichuraiana Rw7G016500 Rw7G016570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 337
Acc36I ACCTGC 1 cut(s) 337
AccB1I GGYRCC 1 cut(s) 212
AccII CGCG 2 cut(s) 89, 237
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 7 cut(s) 237, 240, 275, 289, 392, 449, 588
AclWI GGATC 1 cut(s) 440
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 2 cut(s) 532, 613
AfeI AGCGCT 1 cut(s) 186
AfiI CCNNNNNNNGG 3 cut(s) 174, 209, 291
AgsI TTSAA 2 cut(s) 116, 496
AjnI CCWGG 1 cut(s) 608
AloI GAACNNNNNNTCC 2 cut(s) 210, 242
AluBI AGCT 7 cut(s) 223, 265, 304, 333, 463, 483, 549
AluI AGCT 7 cut(s) 223, 265, 304, 333, 463, 483, 549
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 2 cut(s) 275, 434
AlwI GGATC 1 cut(s) 440
Aor13HI TCCGGA 1 cut(s) 202
Aor51HI AGCGCT 1 cut(s) 186
AoxI GGCC 3 cut(s) 241, 393, 425
ApeKI GCWGC 1 cut(s) 546
ApoI RAATTY 2 cut(s) 532, 613
AspLEI GCGC 2 cut(s) 187, 237
AspS9I GGNCC 1 cut(s) 338
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 1 cut(s) 445
AvaII GGWCC 1 cut(s) 338
BanI GGYRCC 1 cut(s) 212
BanII GRGCYC 3 cut(s) 181, 225, 527
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 1 cut(s) 558
BceAI ACGGC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 610
BclI TGATCA 1 cut(s) 384
BcnI CCSGG 1 cut(s) 172
BcoDI GTCTC 2 cut(s) 275, 434
BfaI CTAG 2 cut(s) 182, 528
BfoI RGCGCY 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 337
BglI GCCNNNNNGGC 1 cut(s) 237
BisI GCNGC 5 cut(s) 238, 241, 275, 393, 547
BlsI GCNGC 5 cut(s) 239, 242, 276, 394, 548
Bme1390I CCNGG 2 cut(s) 172, 610
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 2 cut(s) 172, 610
Bpu10I CCTNAGC 1 cut(s) 334
BpuMI CCSGG 1 cut(s) 172
BsaAI YACGTR 1 cut(s) 409
BsaI GGTCTC 2 cut(s) 275, 434
BsaJI CCNNGG 1 cut(s) 626
BsaWI WCCGGW 2 cut(s) 202, 215
BsaXI ACNNNNNCTCC 4 cut(s) 184, 210, 214, 240
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 209, 291
Bse118I RCCGGY 2 cut(s) 132, 231
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 1 cut(s) 610
BseDI CCNNGG 1 cut(s) 626
BseGI GGATG 1 cut(s) 300
BseLI CCNNNNNNNGG 3 cut(s) 174, 209, 291
BseRI GAGGAG 1 cut(s) 343
BseX3I CGGCCG 1 cut(s) 393
BseXI GCAGC 1 cut(s) 558
BsgI GTGCAG 2 cut(s) 365, 565
Bsh1236I CGCG 2 cut(s) 89, 237
Bsh1285I CGRYCG 2 cut(s) 324, 396
BshFI GGCC 3 cut(s) 243, 395, 427
BshNI GGYRCC 1 cut(s) 212
BsiEI CGRYCG 2 cut(s) 324, 396
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 8 cut(s) 133, 161, 171, 203, 216, 232, 278, 292
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 3 cut(s) 174, 209, 291
BsmAI GTCTC 2 cut(s) 275, 434
BsmFI GGGAC 1 cut(s) 239
BsnI GGCC 3 cut(s) 243, 395, 427
Bso31I GGTCTC 2 cut(s) 275, 434
Bsp1286I GDGCHC 3 cut(s) 181, 225, 527
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 4 cut(s) 119, 321, 384, 445
BspACI CCGC 7 cut(s) 237, 240, 275, 289, 392, 449, 588
BspANI GGCC 3 cut(s) 243, 395, 427
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 2 cut(s) 89, 237
BspLI GGNNCC 1 cut(s) 214
BspMI ACCTGC 1 cut(s) 337
BspPI GGATC 1 cut(s) 440
BspT107I GGYRCC 1 cut(s) 212
BspTNI GGTCTC 2 cut(s) 275, 434
BsrFI RCCGGY 2 cut(s) 132, 231
BssAI RCCGGY 2 cut(s) 132, 231
BssECI CCNNGG 1 cut(s) 626
BssMI GATC 4 cut(s) 119, 321, 384, 445
BssT1I CCWWGG 1 cut(s) 626
Bst2UI CCWGG 1 cut(s) 610
Bst4CI ACNGT 2 cut(s) 373, 455
Bst6I CTCTTC 2 cut(s) 83, 413
BstBAI YACGTR 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 1 cut(s) 334
BstENI CCTNNNNNAGG 1 cut(s) 172
BstF5I GGATG 1 cut(s) 300
BstFNI CGCG 2 cut(s) 89, 237
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 2 cut(s) 187, 237
BstKTI GATC 4 cut(s) 122, 324, 387, 448
BstMAI GTCTC 2 cut(s) 275, 434
BstMBI GATC 4 cut(s) 119, 321, 384, 445
BstMCI CGRYCG 2 cut(s) 324, 396
BstMWI GCNNNNNNNGC 2 cut(s) 237, 271
BstNI CCWGG 1 cut(s) 610
BstSCI CCNGG 2 cut(s) 170, 608
BstUI CGCG 2 cut(s) 89, 237
BstV1I GCAGC 1 cut(s) 558
BstXI CCANNNNNNTGG 1 cut(s) 627
BstZI CGGCCG 1 cut(s) 393
BsuRI GGCC 3 cut(s) 243, 395, 427
BtsCI GGATG 1 cut(s) 300
BveI ACCTGC 1 cut(s) 337
Cac8I GCNNGC 1 cut(s) 233
CfoI GCGC 2 cut(s) 187, 237
Cfr10I RCCGGY 2 cut(s) 132, 231
Cfr13I GGNCC 1 cut(s) 338
CviAII CATG 1 cut(s) 109
DdeI CTNAG 1 cut(s) 334
DpnI GATC 4 cut(s) 121, 323, 386, 447
DpnII GATC 4 cut(s) 119, 321, 384, 445
EaeI YGGCCR 1 cut(s) 393
EagI CGGCCG 1 cut(s) 393
Eam1104I CTCTTC 2 cut(s) 83, 413
EarI CTCTTC 2 cut(s) 83, 413
Ecl136II GAGCTC 1 cut(s) 223
EclXI CGGCCG 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 626
Eco147I AGGCCT 1 cut(s) 427
Eco24I GRGCYC 3 cut(s) 181, 225, 527
Eco31I GGTCTC 2 cut(s) 275, 434
Eco47I GGWCC 1 cut(s) 338
Eco47III AGCGCT 1 cut(s) 186
Eco52I CGGCCG 1 cut(s) 393
Eco53kI GAGCTC 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 338
EcoRI GAATTC 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 608
EcoT14I CCWWGG 1 cut(s) 626
EcoT38I GRGCYC 3 cut(s) 181, 225, 527
ErhI CCWWGG 1 cut(s) 626
FaeI CATG 1 cut(s) 112
FaiI YATR 3 cut(s) 110, 192, 641
FaqI GGGAC 1 cut(s) 239
FatI CATG 1 cut(s) 108
FauI CCCGC 1 cut(s) 581
FbaI TGATCA 1 cut(s) 384
Fnu4HI GCNGC 5 cut(s) 238, 241, 275, 393, 547
FokI GGATG 1 cut(s) 307
FriOI GRGCYC 3 cut(s) 181, 225, 527
Fsp4HI GCNGC 5 cut(s) 238, 241, 275, 393, 547
FspBI CTAG 2 cut(s) 182, 528
GlaI GCGC 2 cut(s) 186, 236
GluI GCNGC 5 cut(s) 238, 241, 275, 393, 547
HaeII RGCGCY 1 cut(s) 188
HaeIII GGCC 3 cut(s) 243, 395, 427
HapII CCGG 8 cut(s) 133, 161, 171, 203, 216, 232, 278, 292
HhaI GCGC 2 cut(s) 187, 237
Hin1II CATG 1 cut(s) 112
Hin6I GCGC 2 cut(s) 185, 235
HinP1I GCGC 2 cut(s) 185, 235
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 1 cut(s) 112
HpaII CCGG 8 cut(s) 133, 161, 171, 203, 216, 232, 278, 292
HphI GGTGA 1 cut(s) 445
Hpy188III TCNNGA 2 cut(s) 203, 325
HpyAV CCTTC 6 cut(s) 76, 317, 417, 430, 490, 595
HpyCH4III ACNGT 2 cut(s) 373, 455
HpyCH4IV ACGT 3 cut(s) 194, 315, 408
HpyCH4V TGCA 2 cut(s) 346, 546
HpyF10VI GCNNNNNNNGC 2 cut(s) 237, 271
HpyF3I CTNAG 1 cut(s) 334
HpySE526I ACGT 3 cut(s) 194, 315, 408
Hsp92II CATG 1 cut(s) 112
HspAI GCGC 2 cut(s) 185, 235
Kpn2I TCCGGA 1 cut(s) 202
KroI GCCGGC 1 cut(s) 231
KroNI GCCGGC 1 cut(s) 233
Ksp22I TGATCA 1 cut(s) 384
Kzo9I GATC 4 cut(s) 119, 321, 384, 445
LmnI GCTCC 2 cut(s) 309, 330
Lsp1109I GCAGC 1 cut(s) 558
MaeI CTAG 2 cut(s) 182, 528
MaeII ACGT 3 cut(s) 194, 315, 408
MaeIII GTNAC 1 cut(s) 316
MalI GATC 4 cut(s) 121, 323, 386, 447
MboI GATC 4 cut(s) 119, 321, 384, 445
MboII GAAGA 2 cut(s) 70, 430
MhlI GDGCHC 3 cut(s) 181, 225, 527
MluCI AATT 4 cut(s) 52, 532, 580, 613
MreI CGCCGGCG 1 cut(s) 231
MroI TCCGGA 1 cut(s) 202
MroNI GCCGGC 1 cut(s) 231
MspI CCGG 8 cut(s) 133, 161, 171, 203, 216, 232, 278, 292
MspR9I CCNGG 2 cut(s) 172, 610
MvaI CCWGG 1 cut(s) 610
MvnI CGCG 2 cut(s) 89, 237
MwoI GCNNNNNNNGC 2 cut(s) 237, 271
NaeI GCCGGC 1 cut(s) 233
NciI CCSGG 1 cut(s) 172
NdeII GATC 4 cut(s) 119, 321, 384, 445
NgoMIV GCCGGC 1 cut(s) 231
NlaIII CATG 1 cut(s) 112
NlaIV GGNNCC 1 cut(s) 214
NmuCI GTSAC 1 cut(s) 316
PaqCI CACCTGC 1 cut(s) 337
PceI AGGCCT 1 cut(s) 427
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PdiI GCCGGC 1 cut(s) 233
PfeI GAWTC 1 cut(s) 112
PfoI TCCNGGA 1 cut(s) 170
PkrI GCNGC 5 cut(s) 239, 242, 276, 394, 548
Ple19I CGATCG 1 cut(s) 324
Ppu21I YACGTR 1 cut(s) 409
PpuMI RGGWCCY 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 608
PspGI CCWGG 1 cut(s) 608
PspN4I GGNNCC 1 cut(s) 214
PspPI GGNCC 1 cut(s) 338
PspPPI RGGWCCY 1 cut(s) 338
PvuI CGATCG 1 cut(s) 324
SacI GAGCTC 1 cut(s) 225
SatI GCNGC 5 cut(s) 238, 241, 275, 393, 547
Sau3AI GATC 4 cut(s) 119, 321, 384, 445
Sau96I GGNCC 1 cut(s) 338
ScrFI CCNGG 2 cut(s) 172, 610
SduI GDGCHC 3 cut(s) 181, 225, 527
SgrAI CRCCGGYG 1 cut(s) 231
SinI GGWCC 1 cut(s) 338
Sse9I AATT 4 cut(s) 52, 532, 580, 613
SseBI AGGCCT 1 cut(s) 427
SsiI CCGC 7 cut(s) 237, 240, 275, 289, 392, 449, 588
SspMI CTAG 2 cut(s) 182, 528
SstI GAGCTC 1 cut(s) 225
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 2 cut(s) 170, 608
StyI CCWWGG 1 cut(s) 626
TaaI ACNGT 2 cut(s) 373, 455
TaiI ACGT 3 cut(s) 197, 318, 411
TaqI TCGA 2 cut(s) 324, 459
TaqII GACCGA 1 cut(s) 458
TasI AATT 4 cut(s) 52, 532, 580, 613
TauI GCSGC 4 cut(s) 240, 243, 277, 395
TfiI GAWTC 1 cut(s) 112
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 1 cut(s) 546
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 2 cut(s) 70, 179
TspGWI ACGGA 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 338
XagI CCTNNNNNAGG 1 cut(s) 172
XapI RAATTY 2 cut(s) 532, 613
XspI CTAG 2 cut(s) 182, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.