Rh7CG198800

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0016839
Physical Location & Seq
Reverse (-)
17637901 .. 17638585
685 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG198800.1

Sequence Viewer

Length: 522 bp
ATGTGTCCTCCGGCCGTTTCCGAGAGGACCCTTCAGACCTTCCCGCCGGAGAGGCACACCAACCTCGTACGCCGGCCGGGAGGTCTCATCTCCATCCCCGGGAGGCTCTGGAAGGCTCCGGAGACCCGCCCGGAGAACTCCCAGTACCGTTTGACGAAGCTGCTTCTGAATGTGACGATAGAGAGGAGCTTGGGGCCTGTACACGTGTTGATGTCGCCGGAGAACACGGTCAGTGATTTGATAAAGTCTGCCGTGGAAATATACGTGAAGGAGAAGAGAAGGCCTTTGGTGAAGGAGACTGATCCGCACCGTTTCGAGCTTCACTACTCACAATTCAGTCTGGAGAGTTTGAAGGAAGATCAGAAGTTGATAAAGTTGGGGTCTAGAAATTTTTTCTTGTGCTCAAAGGCATCAGATTCAGATGCCCTTTTATCTAGCTCTCCTTCGAATTGCTCAAAGGAAGCAAAGAAGGAAATCAATTCTCCATTTCCGTTGATAAAGTTAATGGATTTCTTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

19.69

Weight (kDa)

9.32

Isoelectric Point (pI)

63.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 51 - 134 4.1e-38 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016839)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00830
malus_domestica MD04G1077900.v1.1
prunus_persica Prupe.5G080800_v2.0.a1
pyrus_communis pycom04g06980
rosa_chinensis RchiOBHm_Chr7g0201091
rosa_laevigata RLG00000003692
rosa_multiflora Rmu_sc0000785.1_g000016
rosa_roxburghii Rroxscaffold_3G00255520
rosa_samantha Rh7AG187900 Rh7BG191400 Rh7CG198800 Rh7DG193600
rosa_wichuraiana Rw7G016500 Rw7G016570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 118
AciI CCGC 3 cut(s) 44, 127, 305
AclWI GGATC 1 cut(s) 296
AcoI YGGCCR 2 cut(s) 12, 74
AcsI RAATTY 1 cut(s) 388
AcuI CTGAAG 1 cut(s) 17
AcvI CACGTG 1 cut(s) 205
AfaI GTAC 3 cut(s) 69, 146, 201
AfiI CCNNNNNNNGG 1 cut(s) 99
AflIII ACRYGT 2 cut(s) 202, 204
AgsI TTSAA 1 cut(s) 352
AluBI AGCT 4 cut(s) 160, 189, 319, 438
AluI AGCT 4 cut(s) 160, 189, 319, 438
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 3 cut(s) 89, 116, 290
AlwI GGATC 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 98
Aor13HI TCCGGA 1 cut(s) 118
AoxI GGCC 4 cut(s) 12, 74, 194, 281
ApeKI GCWGC 1 cut(s) 160
ApoI RAATTY 1 cut(s) 388
AspS9I GGNCC 2 cut(s) 27, 194
AsuC2I CCSGG 4 cut(s) 78, 99, 100, 131
AsuHPI GGTGA 1 cut(s) 301
AsuII TTCGAA 1 cut(s) 446
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 1 cut(s) 27
BbrPI CACGTG 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 404
BbvI GCAGC 1 cut(s) 147
BccI CCATC 1 cut(s) 101
BceAI ACGGC 1 cut(s) 236
BcnI CCSGG 4 cut(s) 78, 99, 100, 131
BcoDI GTCTC 3 cut(s) 89, 116, 290
BfaI CTAG 2 cut(s) 384, 435
BglI GCCNNNNNGGC 1 cut(s) 52
BisI GCNGC 1 cut(s) 161
BlsI GCNGC 1 cut(s) 162
Bme1390I CCNGG 4 cut(s) 78, 99, 100, 131
Bme18I GGWCC 1 cut(s) 27
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 2 cut(s) 27, 194
BmiI GGNNCC 3 cut(s) 29, 117, 195
BmrFI CCNGG 4 cut(s) 78, 99, 100, 131
BmrI ACTGGG 1 cut(s) 136
BmsI GCATC 2 cut(s) 412, 419
BmuI ACTGGG 1 cut(s) 136
BpmI CTGGAG 1 cut(s) 362
Bpu14I TTCGAA 1 cut(s) 446
BpuMI CCSGG 4 cut(s) 78, 99, 100, 131
BsaAI YACGTR 2 cut(s) 205, 265
BsaI GGTCTC 2 cut(s) 89, 116
BsaJI CCNNGG 3 cut(s) 97, 98, 252
BsaWI WCCGGW 1 cut(s) 118
Bsc4I CCNNNNNNNGG 1 cut(s) 99
Bse118I RCCGGY 1 cut(s) 72
Bse1I ACTGG 1 cut(s) 142
BseAI TCCGGA 1 cut(s) 118
BseDI CCNNGG 3 cut(s) 97, 98, 252
BseGI GGATG 1 cut(s) 93
BseLI CCNNNNNNNGG 1 cut(s) 99
BseNI ACTGG 1 cut(s) 142
BseRI GAGGAG 1 cut(s) 199
BseX3I CGGCCG 2 cut(s) 12, 74
BseXI GCAGC 1 cut(s) 147
Bsh1285I CGRYCG 2 cut(s) 15, 77
BshFI GGCC 4 cut(s) 14, 76, 196, 283
BsiEI CGRYCG 2 cut(s) 15, 77
BsiHKAI GWGCWC 1 cut(s) 404
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 8 cut(s) 11, 47, 73, 77, 99, 119, 131, 218
BsiWI CGTACG 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 99
BsmAI GTCTC 3 cut(s) 89, 116, 290
BsnI GGCC 4 cut(s) 14, 76, 196, 283
Bso31I GGTCTC 2 cut(s) 89, 116
BsoBI CYCGRG 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 446
Bsp1286I GDGCHC 1 cut(s) 404
Bsp13I TCCGGA 1 cut(s) 118
Bsp1407I TGTACA 1 cut(s) 199
Bsp143I GATC 2 cut(s) 301, 358
BspACI CCGC 3 cut(s) 44, 127, 305
BspANI GGCC 4 cut(s) 14, 76, 196, 283
BspEI TCCGGA 1 cut(s) 118
BspLI GGNNCC 3 cut(s) 29, 117, 195
BspPI GGATC 1 cut(s) 296
BspT104I TTCGAA 1 cut(s) 446
BspTNI GGTCTC 2 cut(s) 89, 116
BsrFI RCCGGY 1 cut(s) 72
BsrGI TGTACA 1 cut(s) 199
BsrI ACTGG 1 cut(s) 142
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 3 cut(s) 97, 98, 252
BssMI GATC 2 cut(s) 301, 358
Bst4CI ACNGT 3 cut(s) 149, 229, 311
Bst6I CTCTTC 1 cut(s) 269
BstAUI TGTACA 1 cut(s) 199
BstBAI YACGTR 2 cut(s) 205, 265
BstBI TTCGAA 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 74
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 93
BstKTI GATC 2 cut(s) 304, 361
BstMAI GTCTC 3 cut(s) 89, 116, 290
BstMBI GATC 2 cut(s) 301, 358
BstMCI CGRYCG 2 cut(s) 15, 77
BstMWI GCNNNNNNNGC 1 cut(s) 52
BstSCI CCNGG 4 cut(s) 76, 97, 98, 129
BstV1I GCAGC 1 cut(s) 147
BstZI CGGCCG 2 cut(s) 12, 74
BsuRI GGCC 4 cut(s) 14, 76, 196, 283
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 74
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 2 cut(s) 27, 194
Cfr9I CCCGGG 1 cut(s) 98
Csp6I GTAC 3 cut(s) 68, 145, 200
CviQI GTAC 3 cut(s) 68, 145, 200
DpnI GATC 2 cut(s) 303, 360
DpnII GATC 2 cut(s) 301, 358
EaeI YGGCCR 2 cut(s) 12, 74
EagI CGGCCG 2 cut(s) 12, 74
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
EclXI CGGCCG 2 cut(s) 12, 74
Eco147I AGGCCT 1 cut(s) 283
Eco31I GGTCTC 2 cut(s) 89, 116
Eco47I GGWCC 1 cut(s) 27
Eco52I CGGCCG 2 cut(s) 12, 74
Eco57I CTGAAG 1 cut(s) 17
Eco72I CACGTG 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 98
EcoO109I RGGNCCY 2 cut(s) 27, 194
FaiI YATR 1 cut(s) 262
FauI CCCGC 2 cut(s) 51, 134
Fnu4HI GCNGC 1 cut(s) 161
FokI GGATG 1 cut(s) 80
Fsp4HI GCNGC 1 cut(s) 161
FspBI CTAG 2 cut(s) 384, 435
GluI GCNGC 1 cut(s) 161
GsuI CTGGAG 1 cut(s) 362
HaeIII GGCC 4 cut(s) 14, 76, 196, 283
HapII CCGG 8 cut(s) 11, 47, 73, 77, 99, 119, 131, 218
HinfI GANTC 1 cut(s) 416
HpaII CCGG 8 cut(s) 11, 47, 73, 77, 99, 119, 131, 218
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 6 cut(s) 22, 36, 168, 363, 415, 421
Hpy188III TCNNGA 4 cut(s) 109, 119, 341, 384
Hpy8I GTNNAC 1 cut(s) 202
HpyAV CCTTC 9 cut(s) 41, 49, 106, 262, 273, 286, 346, 453, 463
HpyCH4III ACNGT 3 cut(s) 149, 229, 311
HpyCH4IV ACGT 2 cut(s) 204, 264
HpyF10VI GCNNNNNNNGC 1 cut(s) 52
HpySE526I ACGT 2 cut(s) 204, 264
Kpn2I TCCGGA 1 cut(s) 118
KroI GCCGGC 1 cut(s) 72
KroNI GCCGGC 1 cut(s) 74
Kzo9I GATC 2 cut(s) 301, 358
LmnI GCTCC 2 cut(s) 121, 186
Lsp1109I GCAGC 1 cut(s) 147
LweI GCATC 2 cut(s) 412, 419
MaeI CTAG 2 cut(s) 384, 435
MaeII ACGT 2 cut(s) 204, 264
MaeIII GTNAC 1 cut(s) 172
MalI GATC 2 cut(s) 303, 360
MboI GATC 2 cut(s) 301, 358
MboII GAAGA 2 cut(s) 286, 368
MhlI GDGCHC 1 cut(s) 404
MluCI AATT 4 cut(s) 332, 388, 448, 478
MnlI CCTC 7 cut(s) 18, 18, 45, 74, 74, 96, 177
MroI TCCGGA 1 cut(s) 118
MroNI GCCGGC 1 cut(s) 72
MseI TTAA 1 cut(s) 503
MspI CCGG 8 cut(s) 11, 47, 73, 77, 99, 119, 131, 218
MspR9I CCNGG 4 cut(s) 78, 99, 100, 131
MwoI GCNNNNNNNGC 1 cut(s) 52
NaeI GCCGGC 1 cut(s) 74
NciI CCSGG 4 cut(s) 78, 99, 100, 131
NdeII GATC 2 cut(s) 301, 358
NgoMIV GCCGGC 1 cut(s) 72
NlaIV GGNNCC 3 cut(s) 29, 117, 195
NmuCI GTSAC 1 cut(s) 172
NspV TTCGAA 1 cut(s) 446
PceI AGGCCT 1 cut(s) 283
PdiI GCCGGC 1 cut(s) 74
PfeI GAWTC 1 cut(s) 416
Pfl23II CGTACG 1 cut(s) 67
PkrI GCNGC 1 cut(s) 162
PmaCI CACGTG 1 cut(s) 205
PmlI CACGTG 1 cut(s) 205
Ppu21I YACGTR 2 cut(s) 205, 265
PpuMI RGGWCCY 1 cut(s) 27
Psp5II RGGWCCY 1 cut(s) 27
PspCI CACGTG 1 cut(s) 205
PspLI CGTACG 1 cut(s) 67
PspN4I GGNNCC 3 cut(s) 29, 117, 195
PspPI GGNCC 2 cut(s) 27, 194
PspPPI RGGWCCY 1 cut(s) 27
PsrI GAACNNNNNNTAC 2 cut(s) 128, 160
RsaI GTAC 3 cut(s) 69, 146, 201
RsaNI GTAC 3 cut(s) 68, 145, 200
SaqAI TTAA 1 cut(s) 503
SatI GCNGC 1 cut(s) 161
Sau3AI GATC 2 cut(s) 301, 358
Sau96I GGNCC 2 cut(s) 27, 194
ScrFI CCNGG 4 cut(s) 78, 99, 100, 131
SduI GDGCHC 1 cut(s) 404
SetI ASST 9 cut(s) 41, 66, 85, 162, 191, 207, 267, 321, 440
SfaNI GCATC 2 cut(s) 412, 419
SfuI TTCGAA 1 cut(s) 446
SinI GGWCC 1 cut(s) 27
SmaI CCCGGG 1 cut(s) 100
Sse9I AATT 4 cut(s) 332, 388, 448, 478
SseBI AGGCCT 1 cut(s) 283
SsiI CCGC 3 cut(s) 44, 127, 305
SspMI CTAG 2 cut(s) 384, 435
StuI AGGCCT 1 cut(s) 283
StyD4I CCNGG 4 cut(s) 76, 97, 98, 129
TaaI ACNGT 3 cut(s) 149, 229, 311
TaiI ACGT 2 cut(s) 207, 267
TaqI TCGA 2 cut(s) 315, 446
TasI AATT 4 cut(s) 332, 388, 448, 478
TatI WGTACW 1 cut(s) 199
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 1 cut(s) 503
Tru9I TTAA 1 cut(s) 503
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 160
Tsp45I GTSAC 1 cut(s) 172
TspGWI ACGGA 1 cut(s) 480
TspMI CCCGGG 1 cut(s) 98
TspRI CASTG 1 cut(s) 238
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 1 cut(s) 388
XbaI TCTAGA 1 cut(s) 383
XmaI CCCGGG 1 cut(s) 98
XspI CTAG 2 cut(s) 384, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.