MD04G1077900.v1.1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016839
Physical Location & Seq
Reverse (-)
10660370 .. 10661483
1114 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1077900.v1.1.491

Sequence Viewer

Length: 486 bp
ATGATCTCCAAGACGGCGCTTCATACGTTTCCGCCCGAGAGGCACAGCGGCAAACTCGTCCGGCATCGAGGAGGCCTCATCTCTGTCCCAGTGAAGCTTTGGCCAGCGCCGGAGACCTACCGAAGGATGACGAAGTTGCTTCTGAACGTGACGATCGAGAGGAGCTTAGGGCCTGTGCAGGTGGTGATGTCGCCGGAAAACACAGTCCGGGATTTGATCAAGGCAGCTGTAGAGATTTACGTGAGGGAGAAGAGAAGGCCGTTGGTAAAGGAGACTGATCCGCACCGTTTTGAGCTTCACTACTCGCAATTCAGCTTGGAGAGTTTGAAAGGAGATGAGAAGTTGATAAAGTTGGGGTCTAGGAATTTCTTTTTGTGCTGCTCAAAGTCAAAGCCCCCCGTCGGTCTGTGTAATTGCTCTGAGGAAGCAAAGAGGGCAATCAGTTATCAATTTTCATGGATAAAGCTCATGGATTTCTTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.55

Weight (kDa)

9.76

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 43 - 126 1.3e-38 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016839)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00830
malus_domestica MD04G1077900.v1.1
prunus_persica Prupe.5G080800_v2.0.a1
pyrus_communis pycom04g06980
rosa_chinensis RchiOBHm_Chr7g0201091
rosa_laevigata RLG00000003692
rosa_multiflora Rmu_sc0000785.1_g000016
rosa_roxburghii Rroxscaffold_3G00255520
rosa_samantha Rh7AG187900 Rh7BG191400 Rh7CG198800 Rh7DG193600
rosa_wichuraiana Rw7G016500 Rw7G016570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 169
AciI CCGC 3 cut(s) 32, 48, 281
AclWI GGATC 1 cut(s) 272
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 1 cut(s) 364
AfiI CCNNNNNNNGG 2 cut(s) 123, 401
AgsI TTSAA 1 cut(s) 328
AluBI AGCT 6 cut(s) 97, 165, 227, 295, 315, 466
AluI AGCT 6 cut(s) 97, 165, 227, 295, 315, 466
Alw26I GTCTC 2 cut(s) 107, 266
AlwI GGATC 1 cut(s) 272
Ama87I CYCGRG 1 cut(s) 35
AoxI GGCC 4 cut(s) 73, 101, 170, 257
ApeKI GCWGC 2 cut(s) 224, 378
ApoI RAATTY 1 cut(s) 364
AspLEI GCGC 2 cut(s) 19, 109
AspS9I GGNCC 1 cut(s) 170
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 35
BalI TGGCCA 1 cut(s) 103
BbvI GCAGC 2 cut(s) 236, 365
BceAI ACGGC 2 cut(s) 30, 244
BclI TGATCA 1 cut(s) 216
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 2 cut(s) 107, 266
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 2 cut(s) 20, 110
BfuAI ACCTGC 1 cut(s) 169
BglI GCCNNNNNGGC 1 cut(s) 40
BisI GCNGC 3 cut(s) 49, 225, 379
BlsI GCNGC 3 cut(s) 50, 226, 380
Bme1390I CCNGG 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 170
BmrFI CCNGG 1 cut(s) 209
BmrI ACTGGG 1 cut(s) 83
BmsI GCATC 1 cut(s) 73
BmuI ACTGGG 1 cut(s) 83
BplI GAGNNNNNCTC 2 cut(s) 60, 92
Bpu10I CCTNAGC 1 cut(s) 166
BpuMI CCSGG 1 cut(s) 209
BsaAI YACGTR 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 107
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 401
Bse1I ACTGG 1 cut(s) 89
BseGI GGATG 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 123, 401
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 2 cut(s) 84, 175
BseXI GCAGC 2 cut(s) 236, 365
BsgI GTGCAG 1 cut(s) 197
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 4 cut(s) 75, 103, 172, 259
BsiEI CGRYCG 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 35
BsiSI CCGG 4 cut(s) 61, 110, 194, 208
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 2 cut(s) 123, 401
BsmAI GTCTC 2 cut(s) 107, 266
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 4 cut(s) 75, 103, 172, 259
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 35
Bsp143I GATC 4 cut(s) 3, 153, 216, 277
BspACI CCGC 3 cut(s) 32, 48, 281
BspANI GGCC 4 cut(s) 75, 103, 172, 259
BspCNI CTCAG 1 cut(s) 412
BspMI ACCTGC 1 cut(s) 169
BspPI GGATC 1 cut(s) 272
BspTNI GGTCTC 1 cut(s) 107
BsrI ACTGG 1 cut(s) 89
BssMI GATC 4 cut(s) 3, 153, 216, 277
Bst4CI ACNGT 2 cut(s) 205, 287
Bst6I CTCTTC 1 cut(s) 245
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 2 cut(s) 166, 420
BstENI CCTNNNNNAGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 132
BstH2I RGCGCY 2 cut(s) 20, 110
BstHHI GCGC 2 cut(s) 19, 109
BstKTI GATC 4 cut(s) 6, 156, 219, 280
BstMAI GTCTC 2 cut(s) 107, 266
BstMBI GATC 4 cut(s) 3, 153, 216, 277
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 2 cut(s) 40, 434
BstSCI CCNGG 1 cut(s) 207
BstSFI CTRYAG 1 cut(s) 228
BstV1I GCAGC 2 cut(s) 236, 365
BsuRI GGCC 4 cut(s) 75, 103, 172, 259
BtsCI GGATG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 96
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 1 cut(s) 105
CfoI GCGC 2 cut(s) 19, 109
Cfr13I GGNCC 1 cut(s) 170
CviAII CATG 2 cut(s) 456, 469
DdeI CTNAG 2 cut(s) 166, 420
DpnI GATC 4 cut(s) 5, 155, 218, 279
DpnII GATC 4 cut(s) 3, 153, 216, 277
EaeI YGGCCR 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
EciI GGCGGA 1 cut(s) 21
Eco147I AGGCCT 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 107
Eco88I CYCGRG 1 cut(s) 35
EcoNI CCTNNNNNAGG 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 170
FaeI CATG 2 cut(s) 459, 472
FaiI YATR 3 cut(s) 24, 457, 470
FaqI GGGAC 1 cut(s) 71
FatI CATG 2 cut(s) 455, 468
FbaI TGATCA 1 cut(s) 216
Fnu4HI GCNGC 3 cut(s) 49, 225, 379
FokI GGATG 1 cut(s) 139
Fsp4HI GCNGC 3 cut(s) 49, 225, 379
FspBI CTAG 1 cut(s) 360
GlaI GCGC 2 cut(s) 18, 108
GluI GCNGC 3 cut(s) 49, 225, 379
HaeII RGCGCY 2 cut(s) 20, 110
HaeIII GGCC 4 cut(s) 75, 103, 172, 259
HapII CCGG 4 cut(s) 61, 110, 194, 208
HhaI GCGC 2 cut(s) 19, 109
Hin1II CATG 2 cut(s) 459, 472
Hin6I GCGC 2 cut(s) 17, 107
HinP1I GCGC 2 cut(s) 17, 107
HindIII AAGCTT 1 cut(s) 95
HpaII CCGG 4 cut(s) 61, 110, 194, 208
HphI GGTGA 1 cut(s) 196
Hpy188I TCNGA 2 cut(s) 144, 421
Hpy188III TCNNGA 1 cut(s) 157
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 2 cut(s) 117, 249
HpyCH4III ACNGT 2 cut(s) 205, 287
HpyCH4IV ACGT 3 cut(s) 26, 147, 240
HpyCH4V TGCA 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 434
HpyF3I CTNAG 2 cut(s) 166, 420
HpySE526I ACGT 3 cut(s) 26, 147, 240
Hsp92II CATG 2 cut(s) 459, 472
HspAI GCGC 2 cut(s) 17, 107
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 4 cut(s) 3, 153, 216, 277
LmnI GCTCC 1 cut(s) 162
LpnPI CCDG 8 cut(s) 74, 102, 117, 123, 164, 186, 207, 221
Lsp1109I GCAGC 2 cut(s) 236, 365
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 360
MaeII ACGT 3 cut(s) 26, 147, 240
MaeIII GTNAC 1 cut(s) 148
MalI GATC 4 cut(s) 5, 155, 218, 279
MboI GATC 4 cut(s) 3, 153, 216, 277
MboII GAAGA 1 cut(s) 262
MlsI TGGCCA 1 cut(s) 103
MluCI AATT 4 cut(s) 308, 364, 412, 449
MluNI TGGCCA 1 cut(s) 103
MnlI CCTC 8 cut(s) 33, 62, 65, 86, 153, 237, 415, 426
Mox20I TGGCCA 1 cut(s) 103
MscI TGGCCA 1 cut(s) 103
Msp20I TGGCCA 1 cut(s) 103
MspA1I CMGCKG 2 cut(s) 48, 227
MspI CCGG 4 cut(s) 61, 110, 194, 208
MspR9I CCNGG 1 cut(s) 209
MwoI GCNNNNNNNGC 2 cut(s) 40, 434
NciI CCSGG 1 cut(s) 209
NdeII GATC 4 cut(s) 3, 153, 216, 277
NlaIII CATG 2 cut(s) 459, 472
NmuCI GTSAC 1 cut(s) 148
PaqCI CACCTGC 1 cut(s) 169
PceI AGGCCT 1 cut(s) 75
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PfoI TCCNGGA 1 cut(s) 207
PkrI GCNGC 3 cut(s) 50, 226, 380
Ple19I CGATCG 1 cut(s) 156
Ppu21I YACGTR 1 cut(s) 241
PspPI GGNCC 1 cut(s) 170
PvuI CGATCG 1 cut(s) 156
PvuII CAGCTG 1 cut(s) 227
SatI GCNGC 3 cut(s) 49, 225, 379
Sau3AI GATC 4 cut(s) 3, 153, 216, 277
Sau96I GGNCC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 209
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 228
Sse9I AATT 4 cut(s) 308, 364, 412, 449
SseBI AGGCCT 1 cut(s) 75
SsiI CCGC 3 cut(s) 32, 48, 281
SspMI CTAG 1 cut(s) 360
StuI AGGCCT 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 207
TaaI ACNGT 2 cut(s) 205, 287
TaiI ACGT 3 cut(s) 29, 150, 243
TaqI TCGA 2 cut(s) 67, 156
TaqII GACCGA 1 cut(s) 392
TasI AATT 4 cut(s) 308, 364, 412, 449
TauI GCSGC 1 cut(s) 51
TscAI CASTG 1 cut(s) 96
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 2 cut(s) 224, 378
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 2 cut(s) 11, 444
TspRI CASTG 1 cut(s) 96
XagI CCTNNNNNAGG 1 cut(s) 121
XapI RAATTY 1 cut(s) 364
XcmI CCANNNNNNNNNTGG 1 cut(s) 96
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.