Rroxscaffold_3G00255520

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016839
Physical Location & Seq
Forward (+)
49845800 .. 49846527
728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00255520.1

Sequence Viewer

Length: 567 bp
ATGATTCATCCGCGGTCACAGTTTTACGGGTGGAACATGCGTCCTCCGGCTGTTTCCGAGAGAACCCTTCAGACCTTCCCGCCGGAGAGGCACACCAACCTCGTACGCCGGCCGGGAGGTCTCATCTCCGTCCCCGGGAGGCTCTGGAAGGCTCCGGAGACCCGCCTGGAGAACTCCCAGTACCGTTTGACGAAGCTGCTTCTGAATGTGACCATAGAGAGGAGCTTGGGGCCTGTACACGTGTTGATGTCGCCGGAGAACACGGTCAGTGATTTGATAAAGTCCGCCGTAGAAATATACGTGAAGGAGAAGAGAAGGCCTTTGGTGAAGGAGACTGATCCGCACCGTTTCGAGCTTCACTACTCACAATTCAGTCTGGAGAGTTTGAAGGAAGATCAGAAGTTGATAAAGTTGGGGTCTAGAAATTTTTTCTTGTGCTCAAAGGCATCAGATTCAGATGCCCTTTTATCTAGCTCTGTCGTCAATCCTTCCAATTGCTCAAAGGAAGCAAAGAAGGAAATCAATTCTCCATTTCCATTGATAAAGTTAATGGATTTCTTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

21.57

Weight (kDa)

9.62

Isoelectric Point (pI)

61.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 63 - 146 5e-38 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016839)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g00830
malus_domestica MD04G1077900.v1.1
prunus_persica Prupe.5G080800_v2.0.a1
pyrus_communis pycom04g06980
rosa_chinensis RchiOBHm_Chr7g0201091
rosa_laevigata RLG00000003692
rosa_multiflora Rmu_sc0000785.1_g000016
rosa_roxburghii Rroxscaffold_3G00255520
rosa_samantha Rh7AG187900 Rh7BG191400 Rh7CG198800 Rh7DG193600
rosa_wichuraiana Rw7G016500 Rw7G016570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 13
AccIII TCCGGA 1 cut(s) 154
AciI CCGC 6 cut(s) 11, 13, 80, 163, 285, 341
AclWI GGATC 1 cut(s) 332
AcoI YGGCCR 1 cut(s) 110
AcsI RAATTY 1 cut(s) 424
AcuI CTGAAG 1 cut(s) 53
AcvI CACGTG 1 cut(s) 241
AfaI GTAC 3 cut(s) 105, 182, 237
AfiI CCNNNNNNNGG 2 cut(s) 135, 219
AflIII ACRYGT 2 cut(s) 238, 240
AgsI TTSAA 1 cut(s) 388
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 4 cut(s) 196, 225, 355, 474
AluI AGCT 4 cut(s) 196, 225, 355, 474
Alw21I GWGCWC 1 cut(s) 440
Alw26I GTCTC 3 cut(s) 125, 152, 326
AlwI GGATC 1 cut(s) 332
Ama87I CYCGRG 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 154
AoxI GGCC 3 cut(s) 110, 230, 317
ApeKI GCWGC 1 cut(s) 196
ApoI RAATTY 1 cut(s) 424
AspS9I GGNCC 1 cut(s) 230
AsuC2I CCSGG 3 cut(s) 114, 135, 136
AsuHPI GGTGA 1 cut(s) 337
AvaI CYCGRG 1 cut(s) 134
BbrPI CACGTG 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 440
BbvI GCAGC 1 cut(s) 183
BceAI ACGGC 1 cut(s) 272
BciT130I CCWGG 1 cut(s) 167
BcnI CCSGG 3 cut(s) 114, 135, 136
BcoDI GTCTC 3 cut(s) 125, 152, 326
BfaI CTAG 2 cut(s) 420, 471
BglI GCCNNNNNGGC 1 cut(s) 88
BisI GCNGC 1 cut(s) 197
BlsI GCNGC 1 cut(s) 198
Bme1390I CCNGG 4 cut(s) 114, 135, 136, 167
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 230
BmiI GGNNCC 2 cut(s) 153, 231
BmrFI CCNGG 4 cut(s) 114, 135, 136, 167
BmrI ACTGGG 1 cut(s) 172
BmsI GCATC 2 cut(s) 448, 455
BmuI ACTGGG 1 cut(s) 172
BpmI CTGGAG 2 cut(s) 188, 398
BpuMI CCSGG 3 cut(s) 114, 135, 136
BsaAI YACGTR 2 cut(s) 241, 301
BsaI GGTCTC 2 cut(s) 125, 152
BsaJI CCNNGG 3 cut(s) 11, 133, 134
BsaWI WCCGGW 1 cut(s) 154
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 219
Bse118I RCCGGY 1 cut(s) 108
Bse1I ACTGG 1 cut(s) 178
BseAI TCCGGA 1 cut(s) 154
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 3 cut(s) 11, 133, 134
BseGI GGATG 1 cut(s) 7
BseLI CCNNNNNNNGG 2 cut(s) 135, 219
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 1 cut(s) 235
BseX3I CGGCCG 1 cut(s) 110
BseXI GCAGC 1 cut(s) 183
Bsh1236I CGCG 1 cut(s) 13
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 3 cut(s) 112, 232, 319
BsiEI CGRYCG 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 440
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 7 cut(s) 47, 83, 109, 113, 135, 155, 254
BsiWI CGTACG 1 cut(s) 103
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 2 cut(s) 135, 219
BsmAI GTCTC 3 cut(s) 125, 152, 326
BsmFI GGGAC 1 cut(s) 116
BsnI GGCC 3 cut(s) 112, 232, 319
Bso31I GGTCTC 2 cut(s) 125, 152
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 440
Bsp13I TCCGGA 1 cut(s) 154
Bsp1407I TGTACA 1 cut(s) 235
Bsp143I GATC 2 cut(s) 337, 394
BspACI CCGC 6 cut(s) 11, 13, 80, 163, 285, 341
BspANI GGCC 3 cut(s) 112, 232, 319
BspEI TCCGGA 1 cut(s) 154
BspFNI CGCG 1 cut(s) 13
BspLI GGNNCC 2 cut(s) 153, 231
BspPI GGATC 1 cut(s) 332
BspTNI GGTCTC 2 cut(s) 125, 152
BsrFI RCCGGY 1 cut(s) 108
BsrGI TGTACA 1 cut(s) 235
BsrI ACTGG 1 cut(s) 178
BssAI RCCGGY 1 cut(s) 108
BssECI CCNNGG 3 cut(s) 11, 133, 134
BssMI GATC 2 cut(s) 337, 394
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 4 cut(s) 21, 185, 265, 347
Bst6I CTCTTC 1 cut(s) 305
BstAUI TGTACA 1 cut(s) 235
BstBAI YACGTR 2 cut(s) 241, 301
BstC8I GCNNGC 1 cut(s) 110
BstDSI CCRYGG 1 cut(s) 11
BstF5I GGATG 1 cut(s) 7
BstFNI CGCG 1 cut(s) 13
BstKTI GATC 2 cut(s) 340, 397
BstMAI GTCTC 3 cut(s) 125, 152, 326
BstMBI GATC 2 cut(s) 337, 394
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNI CCWGG 1 cut(s) 167
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 4 cut(s) 112, 133, 134, 165
BstUI CGCG 1 cut(s) 13
BstV1I GCAGC 1 cut(s) 183
BstZI CGGCCG 1 cut(s) 110
BsuRI GGCC 3 cut(s) 112, 232, 319
BtgI CCRYGG 1 cut(s) 11
BtsCI GGATG 1 cut(s) 7
BtsIMutI CAGTG 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 110
Cfr10I RCCGGY 1 cut(s) 108
Cfr13I GGNCC 1 cut(s) 230
Cfr42I CCGCGG 1 cut(s) 14
Cfr9I CCCGGG 1 cut(s) 134
CseI GACGC 1 cut(s) 29
Csp6I GTAC 3 cut(s) 104, 181, 236
CviAII CATG 1 cut(s) 37
CviQI GTAC 3 cut(s) 104, 181, 236
DpnI GATC 2 cut(s) 339, 396
DpnII GATC 2 cut(s) 337, 394
EaeI YGGCCR 1 cut(s) 110
EagI CGGCCG 1 cut(s) 110
Eam1104I CTCTTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 305
EciI GGCGGA 1 cut(s) 274
EclXI CGGCCG 1 cut(s) 110
Eco147I AGGCCT 1 cut(s) 319
Eco31I GGTCTC 2 cut(s) 125, 152
Eco52I CGGCCG 1 cut(s) 110
Eco57I CTGAAG 1 cut(s) 53
Eco72I CACGTG 1 cut(s) 241
Eco88I CYCGRG 1 cut(s) 134
EcoO109I RGGNCCY 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 165
FaeI CATG 1 cut(s) 40
FaiI YATR 3 cut(s) 38, 215, 298
FaqI GGGAC 1 cut(s) 116
FatI CATG 1 cut(s) 36
FauI CCCGC 2 cut(s) 87, 170
Fnu4HI GCNGC 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 197
FspBI CTAG 2 cut(s) 420, 471
GluI GCNGC 1 cut(s) 197
GsuI CTGGAG 2 cut(s) 188, 398
HaeIII GGCC 3 cut(s) 112, 232, 319
HapII CCGG 7 cut(s) 47, 83, 109, 113, 135, 155, 254
HgaI GACGC 1 cut(s) 29
Hin1II CATG 1 cut(s) 40
HinfI GANTC 2 cut(s) 4, 452
HpaII CCGG 7 cut(s) 47, 83, 109, 113, 135, 155, 254
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 6 cut(s) 58, 72, 204, 399, 451, 457
Hpy188III TCNNGA 4 cut(s) 145, 155, 377, 420
Hpy8I GTNNAC 1 cut(s) 238
HpyAV CCTTC 9 cut(s) 77, 85, 142, 298, 309, 322, 382, 498, 508
HpyCH4III ACNGT 4 cut(s) 21, 185, 265, 347
HpyCH4IV ACGT 2 cut(s) 240, 300
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpySE526I ACGT 2 cut(s) 240, 300
Hsp92II CATG 1 cut(s) 40
Kpn2I TCCGGA 1 cut(s) 154
KroI GCCGGC 1 cut(s) 108
KroNI GCCGGC 1 cut(s) 110
KspI CCGCGG 1 cut(s) 14
Kzo9I GATC 2 cut(s) 337, 394
LmnI GCTCC 2 cut(s) 157, 222
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 2 cut(s) 448, 455
MaeI CTAG 2 cut(s) 420, 471
MaeII ACGT 2 cut(s) 240, 300
MaeIII GTNAC 2 cut(s) 15, 208
MalI GATC 2 cut(s) 339, 396
MboI GATC 2 cut(s) 337, 394
MboII GAAGA 2 cut(s) 322, 404
MfeI CAATTG 1 cut(s) 493
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 4 cut(s) 368, 424, 493, 523
MnlI CCTC 6 cut(s) 54, 81, 110, 110, 132, 213
MroI TCCGGA 1 cut(s) 154
MroNI GCCGGC 1 cut(s) 108
MseI TTAA 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 7 cut(s) 47, 83, 109, 113, 135, 155, 254
MspR9I CCNGG 4 cut(s) 114, 135, 136, 167
MunI CAATTG 1 cut(s) 493
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 1 cut(s) 88
NaeI GCCGGC 1 cut(s) 110
NciI CCSGG 3 cut(s) 114, 135, 136
NdeII GATC 2 cut(s) 337, 394
NgoMIV GCCGGC 1 cut(s) 108
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 2 cut(s) 153, 231
NmuCI GTSAC 2 cut(s) 15, 208
NspI RCATGY 1 cut(s) 40
PceI AGGCCT 1 cut(s) 319
PdiI GCCGGC 1 cut(s) 110
PfeI GAWTC 2 cut(s) 4, 452
Pfl23II CGTACG 1 cut(s) 103
PkrI GCNGC 1 cut(s) 198
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
Ppu21I YACGTR 2 cut(s) 241, 301
Psp6I CCWGG 1 cut(s) 165
PspCI CACGTG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 165
PspLI CGTACG 1 cut(s) 103
PspN4I GGNNCC 2 cut(s) 153, 231
PspPI GGNCC 1 cut(s) 230
PsrI GAACNNNNNNTAC 2 cut(s) 164, 196
RsaI GTAC 3 cut(s) 105, 182, 237
RsaNI GTAC 3 cut(s) 104, 181, 236
SacII CCGCGG 1 cut(s) 14
SaqAI TTAA 1 cut(s) 548
SatI GCNGC 1 cut(s) 197
Sau3AI GATC 2 cut(s) 337, 394
Sau96I GGNCC 1 cut(s) 230
ScrFI CCNGG 4 cut(s) 114, 135, 136, 167
SduI GDGCHC 1 cut(s) 440
SetI ASST 9 cut(s) 77, 102, 121, 198, 227, 243, 303, 357, 476
SfaNI GCATC 2 cut(s) 448, 455
Sfr303I CCGCGG 1 cut(s) 14
SgrBI CCGCGG 1 cut(s) 14
SmaI CCCGGG 1 cut(s) 136
Sse9I AATT 4 cut(s) 368, 424, 493, 523
SseBI AGGCCT 1 cut(s) 319
SsiI CCGC 6 cut(s) 11, 13, 80, 163, 285, 341
SspMI CTAG 2 cut(s) 420, 471
StuI AGGCCT 1 cut(s) 319
StyD4I CCNGG 4 cut(s) 112, 133, 134, 165
TaaI ACNGT 4 cut(s) 21, 185, 265, 347
TaiI ACGT 2 cut(s) 243, 303
TaqI TCGA 1 cut(s) 351
TasI AATT 4 cut(s) 368, 424, 493, 523
TatI WGTACW 1 cut(s) 235
TfiI GAWTC 2 cut(s) 4, 452
Tru1I TTAA 1 cut(s) 548
Tru9I TTAA 1 cut(s) 548
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 2 cut(s) 15, 208
TseI GCWGC 1 cut(s) 196
Tsp45I GTSAC 2 cut(s) 15, 208
TspGWI ACGGA 1 cut(s) 118
TspMI CCCGGG 1 cut(s) 134
TspRI CASTG 1 cut(s) 274
XapI RAATTY 1 cut(s) 424
XbaI TCTAGA 1 cut(s) 419
XceI RCATGY 1 cut(s) 40
XmaI CCCGGG 1 cut(s) 134
XspI CTAG 2 cut(s) 420, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.