FvH4_5g00860

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013944
Physical Location & Seq
Reverse (-)
483384 .. 488127
4744 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00860.t1

Sequence Viewer

Length: 969 bp
ATGGAGGGAAGGGAAGCTGTGAATAGTAGTGGGGTTACAGTGGTGGGATCAGATGCTCCATCAGACTACCACGTGGCGCCGAGGCTCGAAAACCCGGCAAATAACGCCGGATCAGCACCTACGCCGCCGGTTGCGCCGCCGCCCACGGCGGCGACCGTGCCGGCGGCGGCCGGGTCGATGCCGGTGAAGAAGAAGAGAGGGAGGCCGAGGAAGTATGGGCCGGATGGGAGTGTTACTATGGCGCTGTCACCGAAGCCGATATCGTCGGCGGCGCCGCCTCCGGTGATTGATTTCTCGGCGGAGAAGCAGCCGCGGGGGAAAGTGAAGCCGGCCAGCTCGGTGAGCAAGACCAAGTATGAGGTGGAGAATTTAGGTGAATGGGTTGCATGCTCTGTTGGTGCTAATTTTACTCCTCATATCATCAATGTTAATTCTGGCGAGGATGTCATGATGAAGATTATATCATTTTCTCAACAAGGTCCTCGAGCTATTTGTGTGCTCTCCGCAAATGGTGTGATATCAAGTGTTACACTTCGTCAACCTGATTCTTCTGGTGGTACATTGACATATGAGGGTCGCTTTGAGATACTGTCTTTATCTGGTTCCTTTATGCCAAATGAAATTGGAGGGACAAGAAGCAGATCAGGTGGGATGAGTGTTTCTTTAGCAAGTCCAGATGGGCGTGTTGTAGGTGGTGGAGTTGCTGGACTATTAGTTGCTGCAAGTCCTGTGCAGGTTGTAGTTGGCAGTTTTCTGGCGGGAAACCAGCACGAGCAGAAACCCAAGAAACAGAAACATGAATACATTTCTAATGCAACACCAACTATGGCTATTCCTGTTTCAAGTGCTGAGCCAAAACCAAACCTCGCGTCTTCGACTTCCTTCCGAGGTGATAACTGGTCCTCATATCCATCTGATCATAAGAGTAAGACTGACATTAATGTATCAGTGCCTGGAGGTGTAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

323

Amino Acids

33.21

Weight (kDa)

9.37

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 137 - 251 1.9e-26 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12080
fragaria_vesca FvH4_5g00860
malus_domestica MD00G1083100.v1.1 MD06G1063800.v1.1
prunus_persica Prupe.5G081500_v2.0.a1
pyrus_communis pycom04g06900 pycom06g06420
rosa_chinensis RchiOBHm_Chr7g0201041
rosa_laevigata RLG00000003696
rosa_multiflora Rmu_sc0006553.1_g000014
rosa_roxburghii Rroxscaffold_3G00255570
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187500 Rh7BG190800 Rh7CG198500 Rh7DG193100
rosa_wichuraiana Rw7G016450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 724
AccB1I GGYRCC 2 cut(s) 76, 271
AccII CGCG 2 cut(s) 313, 869
AclWI GGATC 2 cut(s) 55, 118
AcoI YGGCCR 2 cut(s) 168, 330
AcsI RAATTY 1 cut(s) 367
AcvI CACGTG 1 cut(s) 73
AcyI GRCGYC 2 cut(s) 77, 272
AfaI GTAC 1 cut(s) 559
AgsI TTSAA 1 cut(s) 843
AjnI CCWGG 1 cut(s) 952
AluBI AGCT 3 cut(s) 17, 336, 488
AluI AGCT 3 cut(s) 17, 336, 488
Alw21I GWGCWC 1 cut(s) 501
AlwI GGATC 2 cut(s) 55, 118
AlwNI CAGNNNCTG 1 cut(s) 953
Ama87I CYCGRG 1 cut(s) 483
AoxI GGCC 4 cut(s) 168, 203, 218, 330
ApeKI GCWGC 2 cut(s) 307, 719
ApoI RAATTY 1 cut(s) 367
ArsI GACNNNNNNTTYG 2 cut(s) 854, 886
AseI ATTAAT 1 cut(s) 939
AspLEI GCGC 4 cut(s) 79, 136, 244, 274
AspS9I GGNCC 3 cut(s) 218, 479, 900
AsuC2I CCSGG 2 cut(s) 95, 172
AsuHPI GGTGA 6 cut(s) 196, 240, 295, 352, 386, 902
AvaI CYCGRG 1 cut(s) 483
AvaII GGWCC 2 cut(s) 479, 900
BanI GGYRCC 2 cut(s) 76, 271
BauI CACGAG 1 cut(s) 770
BbrPI CACGTG 1 cut(s) 73
BbsI GAAGAC 1 cut(s) 864
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 2 cut(s) 319, 706
BccI CCATC 4 cut(s) 67, 218, 671, 919
BceAI ACGGC 1 cut(s) 162
BciT130I CCWGG 1 cut(s) 954
BclI TGATCA 1 cut(s) 916
BcnI CCSGG 2 cut(s) 95, 172
BfoI RGCGCY 3 cut(s) 80, 245, 275
BfuAI ACCTGC 1 cut(s) 724
BlpI GCTNAGC 1 cut(s) 849
Bme1390I CCNGG 3 cut(s) 95, 172, 954
Bme18I GGWCC 2 cut(s) 479, 900
BmeT110I CYCGRG 1 cut(s) 483
BmgT120I GGNCC 3 cut(s) 218, 479, 900
BmiI GGNNCC 3 cut(s) 78, 273, 604
BmrFI CCNGG 3 cut(s) 95, 172, 954
BmsI GCATC 2 cut(s) 43, 168
BpiI GAAGAC 1 cut(s) 864
Bpu1102I GCTNAGC 1 cut(s) 849
BpuMI CCSGG 2 cut(s) 95, 172
BsaAI YACGTR 1 cut(s) 73
BsaHI GRCGYC 2 cut(s) 77, 272
BsaJI CCNNGG 5 cut(s) 80, 144, 206, 311, 886
BsaWI WCCGGW 1 cut(s) 280
Bse118I RCCGGY 4 cut(s) 127, 160, 181, 328
Bse1I ACTGG 1 cut(s) 902
BseBI CCWGG 1 cut(s) 954
BseDI CCNNGG 5 cut(s) 80, 144, 206, 311, 886
BseGI GGATG 3 cut(s) 229, 448, 657
BseMII CTCAG 1 cut(s) 840
BseNI ACTGG 1 cut(s) 902
BseRI GAGGAG 1 cut(s) 402
BseX3I CGGCCG 1 cut(s) 168
BseXI GCAGC 2 cut(s) 319, 706
BsgI GTGCAG 1 cut(s) 752
Bsh1236I CGCG 2 cut(s) 313, 869
Bsh1285I CGRYCG 2 cut(s) 156, 171
BshFI GGCC 4 cut(s) 170, 205, 220, 332
BshNI GGYRCC 2 cut(s) 76, 271
BsiEI CGRYCG 2 cut(s) 156, 171
BsiHKAI GWGCWC 1 cut(s) 501
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 9 cut(s) 95, 108, 128, 161, 171, 182, 221, 281, 329
BslFI GGGAC 1 cut(s) 643
BsmFI GGGAC 1 cut(s) 643
BsnI GGCC 4 cut(s) 170, 205, 220, 332
BsoBI CYCGRG 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 4 cut(s) 47, 110, 641, 916
Bsp1720I GCTNAGC 1 cut(s) 849
BspANI GGCC 4 cut(s) 170, 205, 220, 332
BspCNI CTCAG 1 cut(s) 841
BspFNI CGCG 2 cut(s) 313, 869
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 3 cut(s) 78, 273, 604
BspMI ACCTGC 1 cut(s) 724
BspPI GGATC 2 cut(s) 55, 118
BspT107I GGYRCC 2 cut(s) 76, 271
BsrFI RCCGGY 4 cut(s) 127, 160, 181, 328
BsrI ACTGG 1 cut(s) 902
BssAI RCCGGY 4 cut(s) 127, 160, 181, 328
BssECI CCNNGG 5 cut(s) 80, 144, 206, 311, 886
BssMI GATC 4 cut(s) 47, 110, 641, 916
BssNI GRCGYC 2 cut(s) 77, 272
BssSI CACGAG 1 cut(s) 770
Bst2BI CACGAG 1 cut(s) 770
Bst2UI CCWGG 1 cut(s) 954
Bst4CI ACNGT 3 cut(s) 40, 157, 591
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 2 cut(s) 77, 272
BstBAI YACGTR 1 cut(s) 73
BstC8I GCNNGC 4 cut(s) 162, 330, 334, 388
BstDEI CTNAG 1 cut(s) 849
BstDSI CCRYGG 2 cut(s) 144, 311
BstF5I GGATG 3 cut(s) 229, 448, 657
BstFNI CGCG 2 cut(s) 313, 869
BstH2I RGCGCY 3 cut(s) 80, 245, 275
BstHHI GCGC 4 cut(s) 79, 136, 244, 274
BstKTI GATC 4 cut(s) 50, 113, 644, 919
BstMBI GATC 4 cut(s) 47, 110, 641, 916
BstMCI CGRYCG 2 cut(s) 156, 171
BstMWI GCNNNNNNNGC 4 cut(s) 104, 113, 133, 342
BstNI CCWGG 1 cut(s) 954
BstNSI RCATGY 1 cut(s) 390
BstSCI CCNGG 3 cut(s) 93, 170, 952
BstUI CGCG 2 cut(s) 313, 869
BstV1I GCAGC 2 cut(s) 319, 706
BstV2I GAAGAC 1 cut(s) 864
BstZI CGGCCG 1 cut(s) 168
BsuRI GGCC 4 cut(s) 170, 205, 220, 332
BtgI CCRYGG 2 cut(s) 144, 311
BtsCI GGATG 3 cut(s) 229, 448, 657
BtsIMutI CAGTG 2 cut(s) 45, 954
BveI ACCTGC 1 cut(s) 724
Cac8I GCNNGC 4 cut(s) 162, 330, 334, 388
CaiI CAGNNNCTG 1 cut(s) 953
CciI TCATGA 1 cut(s) 447
CfoI GCGC 4 cut(s) 79, 136, 244, 274
Cfr10I RCCGGY 4 cut(s) 127, 160, 181, 328
Cfr13I GGNCC 3 cut(s) 218, 479, 900
Cfr42I CCGCGG 1 cut(s) 314
CseI GACGC 1 cut(s) 858
Csp6I GTAC 1 cut(s) 558
CviAII CATG 3 cut(s) 387, 448, 797
CviQI GTAC 1 cut(s) 558
DdeI CTNAG 1 cut(s) 849
DinI GGCGCC 2 cut(s) 78, 273
DpnI GATC 4 cut(s) 49, 112, 643, 918
DpnII GATC 4 cut(s) 47, 110, 641, 916
EaeI YGGCCR 2 cut(s) 168, 330
EagI CGGCCG 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 314
EclXI CGGCCG 1 cut(s) 168
Eco32I GATATC 2 cut(s) 261, 519
Eco47I GGWCC 2 cut(s) 479, 900
Eco52I CGGCCG 1 cut(s) 168
Eco72I CACGTG 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 483
EcoO109I RGGNCCY 1 cut(s) 479
EcoRII CCWGG 1 cut(s) 952
EcoRV GATATC 2 cut(s) 261, 519
EgeI GGCGCC 2 cut(s) 78, 273
EheI GGCGCC 2 cut(s) 78, 273
FaeI CATG 3 cut(s) 390, 451, 800
FaqI GGGAC 1 cut(s) 643
FatI CATG 3 cut(s) 386, 447, 796
FauI CCCGC 2 cut(s) 306, 751
FauNDI CATATG 1 cut(s) 568
FbaI TGATCA 1 cut(s) 916
FokI GGATG 3 cut(s) 236, 455, 664
GlaI GCGC 4 cut(s) 78, 135, 243, 273
HaeII RGCGCY 3 cut(s) 80, 245, 275
HaeIII GGCC 4 cut(s) 170, 205, 220, 332
HapII CCGG 9 cut(s) 95, 108, 128, 161, 171, 182, 221, 281, 329
HgaI GACGC 1 cut(s) 858
HhaI GCGC 4 cut(s) 79, 136, 244, 274
Hin1I GRCGYC 2 cut(s) 77, 272
Hin1II CATG 3 cut(s) 390, 451, 800
Hin6I GCGC 4 cut(s) 77, 134, 242, 272
HinP1I GCGC 4 cut(s) 77, 134, 242, 272
HincII GTYRAC 1 cut(s) 539
HindII GTYRAC 1 cut(s) 539
HinfI GANTC 1 cut(s) 545
HpaII CCGG 9 cut(s) 95, 108, 128, 161, 171, 182, 221, 281, 329
HphI GGTGA 6 cut(s) 196, 240, 295, 352, 386, 902
Hpy166II GTNNAC 1 cut(s) 539
Hpy188I TCNGA 4 cut(s) 52, 64, 887, 916
Hpy188III TCNNGA 2 cut(s) 448, 674
Hpy8I GTNNAC 1 cut(s) 539
Hpy99I CGWCG 1 cut(s) 268
HpyAV CCTTC 2 cut(s) 3, 892
HpyCH4III ACNGT 3 cut(s) 40, 157, 591
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 4 cut(s) 386, 722, 733, 815
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 113, 133, 342
HpyF3I CTNAG 1 cut(s) 849
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 2 cut(s) 77, 272
Hsp92II CATG 3 cut(s) 390, 451, 800
HspAI GCGC 4 cut(s) 77, 134, 242, 272
KasI GGCGCC 2 cut(s) 76, 271
KroI GCCGGC 2 cut(s) 160, 328
KroNI GCCGGC 2 cut(s) 162, 330
Ksp22I TGATCA 1 cut(s) 916
KspI CCGCGG 1 cut(s) 314
Kzo9I GATC 4 cut(s) 47, 110, 641, 916
LmnI GCTCC 1 cut(s) 61
Lsp1109I GCAGC 2 cut(s) 319, 706
LweI GCATC 2 cut(s) 43, 168
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 4 cut(s) 34, 232, 246, 526
MalI GATC 4 cut(s) 49, 112, 643, 918
MboI GATC 4 cut(s) 47, 110, 641, 916
MboII GAAGA 6 cut(s) 199, 202, 205, 466, 540, 864
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 5 cut(s) 367, 403, 430, 621, 963
Mly113I GGCGCC 2 cut(s) 77, 272
MroNI GCCGGC 2 cut(s) 160, 328
MseI TTAA 2 cut(s) 429, 939
MspA1I CMGCKG 1 cut(s) 313
MspI CCGG 9 cut(s) 95, 108, 128, 161, 171, 182, 221, 281, 329
MspR9I CCNGG 3 cut(s) 95, 172, 954
MvaI CCWGG 1 cut(s) 954
MvnI CGCG 2 cut(s) 313, 869
MwoI GCNNNNNNNGC 4 cut(s) 104, 113, 133, 342
NaeI GCCGGC 2 cut(s) 162, 330
NarI GGCGCC 2 cut(s) 77, 272
NciI CCSGG 2 cut(s) 95, 172
NdeI CATATG 1 cut(s) 568
NdeII GATC 4 cut(s) 47, 110, 641, 916
NgoMIV GCCGGC 2 cut(s) 160, 328
NlaIII CATG 3 cut(s) 390, 451, 800
NlaIV GGNNCC 3 cut(s) 78, 273, 604
NmeAIII GCCGAG 3 cut(s) 105, 231, 275
NmuCI GTSAC 1 cut(s) 246
NspI RCATGY 1 cut(s) 390
PaeI GCATGC 1 cut(s) 390
PaeR7I CTCGAG 1 cut(s) 483
PagI TCATGA 1 cut(s) 447
PdiI GCCGGC 2 cut(s) 162, 330
PfeI GAWTC 1 cut(s) 545
PluTI GGCGCC 2 cut(s) 80, 275
PmaCI CACGTG 1 cut(s) 73
PmlI CACGTG 1 cut(s) 73
Ppu21I YACGTR 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 479
PshBI ATTAAT 1 cut(s) 939
Psp5II RGGWCCY 1 cut(s) 479
Psp6I CCWGG 1 cut(s) 952
PspCI CACGTG 1 cut(s) 73
PspGI CCWGG 1 cut(s) 952
PspN4I GGNNCC 3 cut(s) 78, 273, 604
PspPI GGNCC 3 cut(s) 218, 479, 900
PspPPI RGGWCCY 1 cut(s) 479
PspXI VCTCGAGB 1 cut(s) 483
PstNI CAGNNNCTG 1 cut(s) 953
RsaI GTAC 1 cut(s) 559
RsaNI GTAC 1 cut(s) 558
SacII CCGCGG 1 cut(s) 314
SaqAI TTAA 2 cut(s) 429, 939
Sau3AI GATC 4 cut(s) 47, 110, 641, 916
Sau96I GGNCC 3 cut(s) 218, 479, 900
ScrFI CCNGG 3 cut(s) 95, 172, 954
SduI GDGCHC 1 cut(s) 501
SfaNI GCATC 2 cut(s) 43, 168
SfoI GGCGCC 2 cut(s) 78, 273
Sfr274I CTCGAG 1 cut(s) 483
Sfr303I CCGCGG 1 cut(s) 314
SgrBI CCGCGG 1 cut(s) 314
SinI GGWCC 2 cut(s) 479, 900
SlaI CTCGAG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 483
SmoI CTYRAG 1 cut(s) 483
SphI GCATGC 1 cut(s) 390
Sse9I AATT 5 cut(s) 367, 403, 430, 621, 963
SspDI GGCGCC 2 cut(s) 76, 271
StyD4I CCNGG 3 cut(s) 93, 170, 952
TaaI ACNGT 3 cut(s) 40, 157, 591
TaiI ACGT 1 cut(s) 75
TaqI TCGA 4 cut(s) 87, 176, 484, 875
TasI AATT 5 cut(s) 367, 403, 430, 621, 963
TauI GCSGC 9 cut(s) 127, 139, 142, 152, 167, 170, 272, 277, 313
TfiI GAWTC 1 cut(s) 545
Tru1I TTAA 2 cut(s) 429, 939
Tru9I TTAA 2 cut(s) 429, 939
TscAI CASTG 2 cut(s) 45, 954
TseFI GTSAC 1 cut(s) 246
TseI GCWGC 2 cut(s) 307, 719
Tsp45I GTSAC 1 cut(s) 246
TspDTI ATGAA 3 cut(s) 467, 633, 813
TspRI CASTG 2 cut(s) 45, 954
VpaK11BI GGWCC 2 cut(s) 479, 900
VspI ATTAAT 1 cut(s) 939
XapI RAATTY 1 cut(s) 367
XceI RCATGY 1 cut(s) 390
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XhoI CTCGAG 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.