Rroxscaffold_3G00255570

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013944
Physical Location & Seq
Reverse (-)
49861229 .. 49865866
4638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00255570.1

Sequence Viewer

Length: 969 bp
ATGGAGGGTAGGGAAGCTGTGAATACTAGTGGGGTTACAGTGGTGGGATCAGATGCTCCGTCAGACTACCACGTGGCTCCGAGGGCCGAAAACCCGGCCAATAACGCCGGATCATCACCTGCGCCGCCGGTTGCTCCGCCGCCCGCGGCGGCGGCGTTGCCGGCGGGGGCAGGGTCCGTGGCGGTGAAGAAGAAGAGAGGGAGGCCGAGGAAGTATGGGCCAGATGGGAGCGTGACTATGGCGCTGTCACCGAAGCCGATATCGTCGGCGGCGCCGCCTCCGGTGATTGATTTCGCGGCGGAGAAGCAGTCGCGGGGGAAAGTGAAGCCGGCCAGCTCAGTGAGCAAGAACAAGTATGAGATGGAGAATTTAGGTGAATGGGTTGCATGCTCTGTTGGTGCTAATTTTACTCCTCATATCATCAATGTTAATTCTGGCGAGGATGTCATGATGAAGATTATATCATTTTCTCAACAAGGTCCTCGAGCTATATGTGTGCTCTCCGCAAATGGTGTTATATCAAGTGTTACACTTCGTCAACCTGATTCTTCTGGTGGTACATTGACATATGAGGGTCGTTTCGAGATACTGTCTTTATCTGGTTCCTTTATGCCAAATGAAAGTGGAGGGACAAGAAGCAGATCAGGTGGGATGAGTGTTTCTTTAGCAAGTCCAGATGGGCGTGTCGTAGGTGGTGGAGTTGCTGGACTATTAGTTGCTGCAAGTCCTGTGCAGGTTGTAGTTGGCAGTTTTCTGGCGGGAAACCAGCATGAGCAGAAACCCAAGAAACAGAAACATGAATACATTTCTAATGCAACACCAACTATGGCTATTCCTGTTTCAAGTGCTGAACCAAAGACAAACCTCTCATCTTCGACTTCCTTCCGTGGTGATAATTGGTCTTCGTATCAATCCGATCATAAAAATAAGACTGACATTAATGTATCATTGCCTGGAGGTGTAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

322

Amino Acids

33.15

Weight (kDa)

9.37

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 137 - 251 1.6e-26 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12080
fragaria_vesca FvH4_5g00860
malus_domestica MD00G1083100.v1.1 MD06G1063800.v1.1
prunus_persica Prupe.5G081500_v2.0.a1
pyrus_communis pycom04g06900 pycom06g06420
rosa_chinensis RchiOBHm_Chr7g0201041
rosa_laevigata RLG00000003696
rosa_multiflora Rmu_sc0006553.1_g000014
rosa_roxburghii Rroxscaffold_3G00255570
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187500 Rh7BG190800 Rh7CG198500 Rh7DG193100
rosa_wichuraiana Rw7G016450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 127
Acc36I ACCTGC 2 cut(s) 127, 724
AccB1I GGYRCC 1 cut(s) 271
AccII CGCG 3 cut(s) 146, 296, 313
AclWI GGATC 2 cut(s) 55, 118
AcoI YGGCCR 2 cut(s) 96, 330
AcsI RAATTY 1 cut(s) 367
AcvI CACGTG 1 cut(s) 73
AcyI GRCGYC 1 cut(s) 272
AfaI GTAC 1 cut(s) 559
AgsI TTSAA 1 cut(s) 843
AhlI ACTAGT 1 cut(s) 26
AjnI CCWGG 1 cut(s) 952
AluBI AGCT 3 cut(s) 17, 336, 488
AluI AGCT 3 cut(s) 17, 336, 488
Alw21I GWGCWC 1 cut(s) 501
AlwI GGATC 2 cut(s) 55, 118
Ama87I CYCGRG 1 cut(s) 483
AoxI GGCC 5 cut(s) 84, 96, 203, 218, 330
ApeKI GCWGC 1 cut(s) 719
ApoI RAATTY 1 cut(s) 367
AseI ATTAAT 1 cut(s) 939
AspLEI GCGC 3 cut(s) 124, 244, 274
AspS9I GGNCC 4 cut(s) 84, 174, 218, 479
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 6 cut(s) 108, 196, 240, 295, 386, 902
AvaI CYCGRG 1 cut(s) 483
AvaII GGWCC 2 cut(s) 174, 479
BanI GGYRCC 1 cut(s) 271
BbrPI CACGTG 1 cut(s) 73
BbsI GAAGAC 1 cut(s) 894
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 1 cut(s) 706
BccI CCATC 3 cut(s) 218, 355, 671
BciT130I CCWGG 1 cut(s) 954
BcnI CCSGG 1 cut(s) 95
BcuI ACTAGT 1 cut(s) 26
BfaI CTAG 1 cut(s) 27
BfoI RGCGCY 2 cut(s) 245, 275
BfuAI ACCTGC 2 cut(s) 127, 724
BisI GCNGC 9 cut(s) 125, 140, 147, 150, 153, 270, 275, 297, 720
BlsI GCNGC 9 cut(s) 126, 141, 148, 151, 154, 271, 276, 298, 721
Bme1390I CCNGG 2 cut(s) 95, 954
Bme18I GGWCC 2 cut(s) 174, 479
BmeT110I CYCGRG 1 cut(s) 483
BmgT120I GGNCC 4 cut(s) 84, 174, 218, 479
BmiI GGNNCC 4 cut(s) 78, 175, 273, 604
BmrFI CCNGG 2 cut(s) 95, 954
BmsI GCATC 1 cut(s) 43
BpiI GAAGAC 1 cut(s) 894
BpuMI CCSGG 1 cut(s) 95
BsaAI YACGTR 1 cut(s) 73
BsaHI GRCGYC 1 cut(s) 272
BsaJI CCNNGG 5 cut(s) 80, 144, 177, 206, 886
BsaWI WCCGGW 1 cut(s) 280
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bse118I RCCGGY 3 cut(s) 127, 160, 328
Bse3DI GCAATG 1 cut(s) 947
BseBI CCWGG 1 cut(s) 954
BseDI CCNNGG 5 cut(s) 80, 144, 177, 206, 886
BseGI GGATG 2 cut(s) 448, 657
BseMI GCAATG 1 cut(s) 947
BseMII CTCAG 1 cut(s) 351
BseRI GAGGAG 1 cut(s) 402
BseXI GCAGC 1 cut(s) 706
BsgI GTGCAG 1 cut(s) 752
Bsh1236I CGCG 3 cut(s) 146, 296, 313
BshFI GGCC 5 cut(s) 86, 98, 205, 220, 332
BshNI GGYRCC 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 501
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 6 cut(s) 95, 108, 128, 161, 281, 329
BslFI GGGAC 1 cut(s) 643
BsmFI GGGAC 1 cut(s) 643
BsnI GGCC 5 cut(s) 86, 98, 205, 220, 332
BsoBI CYCGRG 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 4 cut(s) 47, 110, 641, 916
BspANI GGCC 5 cut(s) 86, 98, 205, 220, 332
BspCNI CTCAG 1 cut(s) 350
BspFNI CGCG 3 cut(s) 146, 296, 313
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 4 cut(s) 78, 175, 273, 604
BspMI ACCTGC 2 cut(s) 127, 724
BspPI GGATC 2 cut(s) 55, 118
BspT107I GGYRCC 1 cut(s) 271
BsrDI GCAATG 1 cut(s) 947
BsrFI RCCGGY 3 cut(s) 127, 160, 328
BssAI RCCGGY 3 cut(s) 127, 160, 328
BssECI CCNNGG 5 cut(s) 80, 144, 177, 206, 886
BssMI GATC 4 cut(s) 47, 110, 641, 916
BssNI GRCGYC 1 cut(s) 272
Bst2UI CCWGG 1 cut(s) 954
Bst4CI ACNGT 2 cut(s) 40, 591
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 1 cut(s) 272
BstBAI YACGTR 1 cut(s) 73
BstC8I GCNNGC 5 cut(s) 144, 162, 330, 334, 388
BstDEI CTNAG 1 cut(s) 337
BstDSI CCRYGG 3 cut(s) 144, 177, 886
BstF5I GGATG 2 cut(s) 448, 657
BstFNI CGCG 3 cut(s) 146, 296, 313
BstH2I RGCGCY 2 cut(s) 245, 275
BstHHI GCGC 3 cut(s) 124, 244, 274
BstKTI GATC 4 cut(s) 50, 113, 644, 919
BstMBI GATC 4 cut(s) 47, 110, 641, 916
BstMWI GCNNNNNNNGC 5 cut(s) 83, 104, 152, 161, 342
BstNI CCWGG 1 cut(s) 954
BstNSI RCATGY 1 cut(s) 390
BstSCI CCNGG 2 cut(s) 93, 952
BstUI CGCG 3 cut(s) 146, 296, 313
BstV1I GCAGC 1 cut(s) 706
BstV2I GAAGAC 1 cut(s) 894
BsuRI GGCC 5 cut(s) 86, 98, 205, 220, 332
BtgI CCRYGG 3 cut(s) 144, 177, 886
BtsCI GGATG 2 cut(s) 448, 657
BtsIMutI CAGTG 2 cut(s) 45, 345
BveI ACCTGC 2 cut(s) 127, 724
Cac8I GCNNGC 5 cut(s) 144, 162, 330, 334, 388
CciI TCATGA 1 cut(s) 447
CfoI GCGC 3 cut(s) 124, 244, 274
Cfr10I RCCGGY 3 cut(s) 127, 160, 328
Cfr13I GGNCC 4 cut(s) 84, 174, 218, 479
Cfr42I CCGCGG 1 cut(s) 147
Csp6I GTAC 1 cut(s) 558
CviAII CATG 4 cut(s) 387, 448, 770, 797
CviQI GTAC 1 cut(s) 558
DdeI CTNAG 1 cut(s) 337
DinI GGCGCC 1 cut(s) 273
DpnI GATC 4 cut(s) 49, 112, 643, 918
DpnII GATC 4 cut(s) 47, 110, 641, 916
EaeI YGGCCR 2 cut(s) 96, 330
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 2 cut(s) 126, 314
Eco32I GATATC 1 cut(s) 261
Eco47I GGWCC 2 cut(s) 174, 479
Eco72I CACGTG 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 483
EcoO109I RGGNCCY 1 cut(s) 479
EcoRII CCWGG 1 cut(s) 952
EcoRV GATATC 1 cut(s) 261
EgeI GGCGCC 1 cut(s) 273
EheI GGCGCC 1 cut(s) 273
FaeI CATG 4 cut(s) 390, 451, 773, 800
FaqI GGGAC 1 cut(s) 643
FatI CATG 4 cut(s) 386, 447, 769, 796
FauI CCCGC 4 cut(s) 151, 157, 306, 751
FauNDI CATATG 1 cut(s) 568
Fnu4HI GCNGC 9 cut(s) 125, 140, 147, 150, 153, 270, 275, 297, 720
FokI GGATG 2 cut(s) 455, 664
Fsp4HI GCNGC 9 cut(s) 125, 140, 147, 150, 153, 270, 275, 297, 720
FspBI CTAG 1 cut(s) 27
GlaI GCGC 3 cut(s) 123, 243, 273
GluI GCNGC 9 cut(s) 125, 140, 147, 150, 153, 270, 275, 297, 720
HaeII RGCGCY 2 cut(s) 245, 275
HaeIII GGCC 5 cut(s) 86, 98, 205, 220, 332
HapII CCGG 6 cut(s) 95, 108, 128, 161, 281, 329
HhaI GCGC 3 cut(s) 124, 244, 274
Hin1I GRCGYC 1 cut(s) 272
Hin1II CATG 4 cut(s) 390, 451, 773, 800
Hin6I GCGC 3 cut(s) 122, 242, 272
HinP1I GCGC 3 cut(s) 122, 242, 272
HincII GTYRAC 1 cut(s) 539
HindII GTYRAC 1 cut(s) 539
HinfI GANTC 1 cut(s) 545
HpaII CCGG 6 cut(s) 95, 108, 128, 161, 281, 329
HphI GGTGA 6 cut(s) 108, 196, 240, 295, 386, 902
Hpy166II GTNNAC 1 cut(s) 539
Hpy188I TCNGA 4 cut(s) 52, 64, 81, 916
Hpy188III TCNNGA 3 cut(s) 448, 583, 674
Hpy8I GTNNAC 1 cut(s) 539
Hpy99I CGWCG 1 cut(s) 268
HpyAV CCTTC 1 cut(s) 892
HpyCH4III ACNGT 2 cut(s) 40, 591
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 4 cut(s) 386, 722, 733, 815
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 104, 152, 161, 342
HpyF3I CTNAG 1 cut(s) 337
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 272
Hsp92II CATG 4 cut(s) 390, 451, 773, 800
HspAI GCGC 3 cut(s) 122, 242, 272
KasI GGCGCC 1 cut(s) 271
KroI GCCGGC 2 cut(s) 160, 328
KroNI GCCGGC 2 cut(s) 162, 330
KspI CCGCGG 1 cut(s) 147
Kzo9I GATC 4 cut(s) 47, 110, 641, 916
LmnI GCTCC 4 cut(s) 61, 82, 139, 228
Lsp1109I GCAGC 1 cut(s) 706
LweI GCATC 1 cut(s) 43
MaeI CTAG 1 cut(s) 27
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 4 cut(s) 34, 232, 246, 526
MalI GATC 4 cut(s) 49, 112, 643, 918
MboI GATC 4 cut(s) 47, 110, 641, 916
MboII GAAGA 7 cut(s) 199, 202, 205, 466, 540, 864, 894
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 4 cut(s) 367, 403, 430, 895
Mly113I GGCGCC 1 cut(s) 272
MroNI GCCGGC 2 cut(s) 160, 328
MseI TTAA 2 cut(s) 429, 939
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 6 cut(s) 95, 108, 128, 161, 281, 329
MspR9I CCNGG 2 cut(s) 95, 954
MvaI CCWGG 1 cut(s) 954
MvnI CGCG 3 cut(s) 146, 296, 313
MwoI GCNNNNNNNGC 5 cut(s) 83, 104, 152, 161, 342
NaeI GCCGGC 2 cut(s) 162, 330
NarI GGCGCC 1 cut(s) 272
NciI CCSGG 1 cut(s) 95
NdeI CATATG 1 cut(s) 568
NdeII GATC 4 cut(s) 47, 110, 641, 916
NgoMIV GCCGGC 2 cut(s) 160, 328
NlaIII CATG 4 cut(s) 390, 451, 773, 800
NlaIV GGNNCC 4 cut(s) 78, 175, 273, 604
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 2 cut(s) 232, 246
NspI RCATGY 1 cut(s) 390
PaeI GCATGC 1 cut(s) 390
PaeR7I CTCGAG 1 cut(s) 483
PagI TCATGA 1 cut(s) 447
PaqCI CACCTGC 1 cut(s) 127
PdiI GCCGGC 2 cut(s) 162, 330
PfeI GAWTC 1 cut(s) 545
PkrI GCNGC 9 cut(s) 126, 141, 148, 151, 154, 271, 276, 298, 721
PluTI GGCGCC 1 cut(s) 275
PmaCI CACGTG 1 cut(s) 73
PmlI CACGTG 1 cut(s) 73
Ppu21I YACGTR 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 479
PshBI ATTAAT 1 cut(s) 939
Psp5II RGGWCCY 1 cut(s) 479
Psp6I CCWGG 1 cut(s) 952
PspCI CACGTG 1 cut(s) 73
PspGI CCWGG 1 cut(s) 952
PspN4I GGNNCC 4 cut(s) 78, 175, 273, 604
PspPI GGNCC 4 cut(s) 84, 174, 218, 479
PspPPI RGGWCCY 1 cut(s) 479
PspXI VCTCGAGB 1 cut(s) 483
RsaI GTAC 1 cut(s) 559
RsaNI GTAC 1 cut(s) 558
SacII CCGCGG 1 cut(s) 147
SaqAI TTAA 2 cut(s) 429, 939
SatI GCNGC 9 cut(s) 125, 140, 147, 150, 153, 270, 275, 297, 720
Sau3AI GATC 4 cut(s) 47, 110, 641, 916
Sau96I GGNCC 4 cut(s) 84, 174, 218, 479
ScrFI CCNGG 2 cut(s) 95, 954
SduI GDGCHC 1 cut(s) 501
SfaNI GCATC 1 cut(s) 43
SfoI GGCGCC 1 cut(s) 273
Sfr274I CTCGAG 1 cut(s) 483
Sfr303I CCGCGG 1 cut(s) 147
SgrBI CCGCGG 1 cut(s) 147
SinI GGWCC 2 cut(s) 174, 479
SlaI CTCGAG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 483
SmoI CTYRAG 1 cut(s) 483
SpeI ACTAGT 1 cut(s) 26
SphI GCATGC 1 cut(s) 390
Sse9I AATT 4 cut(s) 367, 403, 430, 895
SspDI GGCGCC 1 cut(s) 271
SspMI CTAG 1 cut(s) 27
StyD4I CCNGG 2 cut(s) 93, 952
TaaI ACNGT 2 cut(s) 40, 591
TaiI ACGT 1 cut(s) 75
TaqI TCGA 3 cut(s) 484, 582, 875
TasI AATT 4 cut(s) 367, 403, 430, 895
TauI GCSGC 8 cut(s) 127, 142, 149, 152, 155, 272, 277, 299
TfiI GAWTC 1 cut(s) 545
Tru1I TTAA 2 cut(s) 429, 939
Tru9I TTAA 2 cut(s) 429, 939
TscAI CASTG 2 cut(s) 45, 345
TseFI GTSAC 2 cut(s) 232, 246
TseI GCWGC 1 cut(s) 719
Tsp45I GTSAC 2 cut(s) 232, 246
TspDTI ATGAA 3 cut(s) 467, 633, 813
TspGWI ACGGA 3 cut(s) 48, 166, 875
TspRI CASTG 2 cut(s) 45, 345
VpaK11BI GGWCC 2 cut(s) 174, 479
VspI ATTAAT 1 cut(s) 939
XapI RAATTY 1 cut(s) 367
XceI RCATGY 1 cut(s) 390
XhoI CTCGAG 1 cut(s) 483
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.