MD06G1063800.v1.1

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013944
Physical Location & Seq
Forward (+)
11030813 .. 11035904
5092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1063800.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGAGGGTAGGGAAGGTGTGAATAGTAGTGGGGTTACAGTTGTGGGATCAGATGCTCCATCAGACTACCATGTCGCTCCAAGGTCCGAAAACCCGACCCACAACGCCGGATCAACACCTCAGGCGCCGGTTGCTCCGCCACAGGCGGCGGCAGGGTCTATGCCGATGAAGAAGAAGAGAGGGAGGCCAAGGAAGTATGGACCGGACGGGAGTGTCACCATGGCTCTGTCACCGAAGCCCATATCGTCCTCTGCACCGCCGCCGCCGCCGGTGATCGATTTCTCGGCGGAGAAGCGTGGGAAAGTGAAGCCAAGCTCAGTGAGCAAAACCAAGTACGAGGTGGAGAATTTAGGTGAATGGGTTGCATGCTCAGTCGGTGCTAATTTTACACCCCATATCATCACTGTTAACTCCGGCGAGGATGTCATGATGAAGATTATATCCTTTTCTCAACAAGGTCCTCGAGCTATATGCGTGCTCTCTGCCAATGGTGTTATTTCAAGTGTGACTCTTCGTCAGCCTGATTCTTCTGGTGGTACATTGACATATGAGGGCCGTTTCGAGATATTGTCTTTGTCTGGTTCATTTATGCCCAATGAAACGGGAGGGACAAGAAGCAGATCAGGTGGGATGAGCGTTTCTTTGGCAAGTCCAGATGGGCGTGTTGTAGGTGGTGGAGTGGCTGGTCTGTTAGTAGCTGCAAGTCCTGTGCAGGTTGTAGTAGGCAGTTTTCTATCGGGAAACCAGCATGAGCAGAAGCCCAAGAAGCAGAAACACGATTACATCTCTAATGCAACACCAACTATGGCTGTTCCTATTTCGAGCGCTGAACCAAAACCAAACTTCTCATCCTCAACTTCTTTCCGTGGAGATAATTGGTCTTCACTGCCATCAGATTCAAAAAACAAGACTGACATTAATGTATCGTTGCCAGGAGGCGTAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

316

Amino Acids

32.68

Weight (kDa)

9.39

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 130 - 244 1.2e-26 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12080
fragaria_vesca FvH4_5g00860
malus_domestica MD00G1083100.v1.1 MD06G1063800.v1.1
prunus_persica Prupe.5G081500_v2.0.a1
pyrus_communis pycom04g06900 pycom06g06420
rosa_chinensis RchiOBHm_Chr7g0201041
rosa_laevigata RLG00000003696
rosa_multiflora Rmu_sc0006553.1_g000014
rosa_roxburghii Rroxscaffold_3G00255570
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187500 Rh7BG190800 Rh7CG198500 Rh7DG193100
rosa_wichuraiana Rw7G016450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 71, 212
Acc36I ACCTGC 1 cut(s) 703
AccB1I GGYRCC 1 cut(s) 124
AciI CCGC 8 cut(s) 137, 146, 149, 257, 260, 263, 266, 287
AclWI GGATC 2 cut(s) 55, 118
AcsI RAATTY 1 cut(s) 346
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 2 cut(s) 335, 538
AfeI AGCGCT 1 cut(s) 826
AgsI TTSAA 2 cut(s) 501, 900
AhdI GACNNNNNGTC 1 cut(s) 513
AjnI CCWGG 1 cut(s) 931
AluBI AGCT 3 cut(s) 315, 467, 698
AluI AGCT 3 cut(s) 315, 467, 698
Alw21I GWGCWC 1 cut(s) 480
AlwI GGATC 2 cut(s) 55, 118
Ama87I CYCGRG 1 cut(s) 462
Aor51HI AGCGCT 1 cut(s) 826
AoxI GGCC 2 cut(s) 185, 553
ApeKI GCWGC 1 cut(s) 698
ApoI RAATTY 1 cut(s) 346
AseI ATTAAT 1 cut(s) 918
AspLEI GCGC 2 cut(s) 127, 827
AspS9I GGNCC 4 cut(s) 84, 200, 458, 553
AsuHPI GGTGA 4 cut(s) 208, 222, 283, 365
AvaI CYCGRG 1 cut(s) 462
AvaII GGWCC 3 cut(s) 84, 200, 458
AxyI CCTNAGG 1 cut(s) 120
BanI GGYRCC 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 873
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 1 cut(s) 685
BccI CCATC 3 cut(s) 67, 650, 898
BceAI ACGGC 1 cut(s) 540
BcgI CGANNNNNNTGC 2 cut(s) 453, 487
BciT130I CCWGG 1 cut(s) 933
BfoI RGCGCY 2 cut(s) 128, 828
BfuAI ACCTGC 1 cut(s) 703
BisI GCNGC 6 cut(s) 147, 150, 260, 263, 266, 699
BlsI GCNGC 6 cut(s) 148, 151, 261, 264, 267, 700
Bme1390I CCNGG 1 cut(s) 933
Bme18I GGWCC 3 cut(s) 84, 200, 458
BmeRI GACNNNNNGTC 1 cut(s) 513
BmeT110I CYCGRG 1 cut(s) 462
BmgT120I GGNCC 4 cut(s) 84, 200, 458, 553
BmiI GGNNCC 1 cut(s) 126
BmrFI CCNGG 1 cut(s) 933
BmsI GCATC 1 cut(s) 43
BpiI GAAGAC 1 cut(s) 873
Bsa29I ATCGAT 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 4 cut(s) 80, 188, 219, 865
BsaWI WCCGGW 1 cut(s) 202
Bse118I RCCGGY 2 cut(s) 127, 268
Bse21I CCTNAGG 1 cut(s) 120
BseBI CCWGG 1 cut(s) 933
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 4 cut(s) 80, 188, 219, 865
BseGI GGATG 3 cut(s) 427, 636, 848
BseMII CTCAG 3 cut(s) 134, 330, 384
BseXI GCAGC 1 cut(s) 685
BsgI GTGCAG 2 cut(s) 237, 731
BshFI GGCC 2 cut(s) 187, 555
BshNI GGYRCC 1 cut(s) 124
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 480
BsiHKCI CYCGRG 1 cut(s) 462
BsiSI CCGG 5 cut(s) 108, 128, 203, 269, 414
BslFI GGGAC 1 cut(s) 622
BsmFI GGGAC 1 cut(s) 622
BsnI GGCC 2 cut(s) 187, 555
BsoBI CYCGRG 1 cut(s) 462
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 4 cut(s) 47, 110, 273, 620
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 8 cut(s) 137, 146, 149, 257, 260, 263, 266, 287
BspANI GGCC 2 cut(s) 187, 555
BspCNI CTCAG 3 cut(s) 133, 329, 383
BspDI ATCGAT 1 cut(s) 276
BspHI TCATGA 1 cut(s) 426
BspLI GGNNCC 1 cut(s) 126
BspMI ACCTGC 1 cut(s) 703
BspPI GGATC 2 cut(s) 55, 118
BspT107I GGYRCC 1 cut(s) 124
BsrFI RCCGGY 2 cut(s) 127, 268
BssAI RCCGGY 2 cut(s) 127, 268
BssECI CCNNGG 4 cut(s) 80, 188, 219, 865
BssMI GATC 4 cut(s) 47, 110, 273, 620
BssNI GRCGYC 1 cut(s) 125
BssT1I CCWWGG 3 cut(s) 80, 188, 219
Bst2UI CCWGG 1 cut(s) 933
Bst4CI ACNGT 2 cut(s) 40, 406
Bst6I CTCTTC 2 cut(s) 170, 516
BstACI GRCGYC 1 cut(s) 125
BstC8I GCNNGC 2 cut(s) 367, 476
BstDEI CTNAG 3 cut(s) 120, 316, 370
BstDSI CCRYGG 2 cut(s) 219, 865
BstF5I GGATG 3 cut(s) 427, 636, 848
BstH2I RGCGCY 2 cut(s) 128, 828
BstHHI GCGC 2 cut(s) 127, 827
BstKTI GATC 4 cut(s) 50, 113, 276, 623
BstMBI GATC 4 cut(s) 47, 110, 273, 620
BstMWI GCNNNNNNNGC 4 cut(s) 131, 265, 321, 766
BstNI CCWGG 1 cut(s) 933
BstNSI RCATGY 1 cut(s) 369
BstSCI CCNGG 1 cut(s) 931
BstV1I GCAGC 1 cut(s) 685
BstV2I GAAGAC 1 cut(s) 873
Bsu15I ATCGAT 1 cut(s) 276
Bsu36I CCTNAGG 1 cut(s) 120
BsuRI GGCC 2 cut(s) 187, 555
BsuTUI ATCGAT 1 cut(s) 276
BtgI CCRYGG 2 cut(s) 219, 865
BtsCI GGATG 3 cut(s) 427, 636, 848
BtsI GCAGTG 1 cut(s) 884
BtsIMutI CAGTG 3 cut(s) 324, 402, 884
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 2 cut(s) 367, 476
CciI TCATGA 1 cut(s) 426
CfoI GCGC 2 cut(s) 127, 827
Cfr10I RCCGGY 2 cut(s) 127, 268
Cfr13I GGNCC 4 cut(s) 84, 200, 458, 553
ClaI ATCGAT 1 cut(s) 276
Csp6I GTAC 2 cut(s) 334, 537
CviAII CATG 5 cut(s) 71, 220, 366, 427, 749
CviQI GTAC 2 cut(s) 334, 537
DdeI CTNAG 3 cut(s) 120, 316, 370
DinI GGCGCC 1 cut(s) 126
DpnI GATC 4 cut(s) 49, 112, 275, 622
DpnII GATC 4 cut(s) 47, 110, 273, 620
DrdI GACNNNNNNGTC 2 cut(s) 71, 212
DriI GACNNNNNGTC 1 cut(s) 513
DseDI GACNNNNNNGTC 2 cut(s) 71, 212
Eam1104I CTCTTC 2 cut(s) 170, 516
Eam1105I GACNNNNNGTC 1 cut(s) 513
EarI CTCTTC 2 cut(s) 170, 516
EciI GGCGGA 2 cut(s) 126, 302
Eco130I CCWWGG 3 cut(s) 80, 188, 219
Eco47I GGWCC 3 cut(s) 84, 200, 458
Eco47III AGCGCT 1 cut(s) 826
Eco81I CCTNAGG 1 cut(s) 120
Eco88I CYCGRG 1 cut(s) 462
EcoO109I RGGNCCY 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 931
EcoT14I CCWWGG 3 cut(s) 80, 188, 219
EgeI GGCGCC 1 cut(s) 126
EheI GGCGCC 1 cut(s) 126
ErhI CCWWGG 3 cut(s) 80, 188, 219
FaeI CATG 5 cut(s) 74, 223, 369, 430, 752
FaqI GGGAC 1 cut(s) 622
FatI CATG 5 cut(s) 70, 219, 365, 426, 748
FauNDI CATATG 1 cut(s) 547
Fnu4HI GCNGC 6 cut(s) 147, 150, 260, 263, 266, 699
FokI GGATG 3 cut(s) 434, 643, 835
Fsp4HI GCNGC 6 cut(s) 147, 150, 260, 263, 266, 699
GlaI GCGC 2 cut(s) 126, 826
GluI GCNGC 6 cut(s) 147, 150, 260, 263, 266, 699
HaeII RGCGCY 2 cut(s) 128, 828
HaeIII GGCC 2 cut(s) 187, 555
HapII CCGG 5 cut(s) 108, 128, 203, 269, 414
HhaI GCGC 2 cut(s) 127, 827
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 5 cut(s) 74, 223, 369, 430, 752
Hin6I GCGC 2 cut(s) 125, 825
HinP1I GCGC 2 cut(s) 125, 825
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 3 cut(s) 508, 524, 896
HpaI GTTAAC 1 cut(s) 409
HpaII CCGG 5 cut(s) 108, 128, 203, 269, 414
HphI GGTGA 4 cut(s) 208, 222, 283, 365
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 4 cut(s) 52, 64, 88, 895
Hpy188III TCNNGA 4 cut(s) 427, 562, 653, 738
Hpy8I GTNNAC 1 cut(s) 409
HpyAV CCTTC 1 cut(s) 8
HpyCH4III ACNGT 2 cut(s) 40, 406
HpyCH4V TGCA 5 cut(s) 254, 365, 701, 712, 794
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 265, 321, 766
HpyF3I CTNAG 3 cut(s) 120, 316, 370
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 5 cut(s) 74, 223, 369, 430, 752
HspAI GCGC 2 cut(s) 125, 825
KasI GGCGCC 1 cut(s) 124
KspAI GTTAAC 1 cut(s) 409
Kzo9I GATC 4 cut(s) 47, 110, 273, 620
LmnI GCTCC 3 cut(s) 61, 82, 139
Lsp1109I GCAGC 1 cut(s) 685
LweI GCATC 1 cut(s) 43
MaeIII GTNAC 4 cut(s) 34, 214, 228, 505
MalI GATC 4 cut(s) 49, 112, 275, 622
MboI GATC 4 cut(s) 47, 110, 273, 620
MboII GAAGA 7 cut(s) 181, 184, 187, 445, 503, 519, 873
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 3 cut(s) 346, 382, 874
Mly113I GGCGCC 1 cut(s) 125
MlyI GAGTC 1 cut(s) 502
MseI TTAA 2 cut(s) 408, 918
MspI CCGG 5 cut(s) 108, 128, 203, 269, 414
MspR9I CCNGG 1 cut(s) 933
MvaI CCWGG 1 cut(s) 933
MwoI GCNNNNNNNGC 4 cut(s) 131, 265, 321, 766
NarI GGCGCC 1 cut(s) 125
NcoI CCATGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 547
NdeII GATC 4 cut(s) 47, 110, 273, 620
NlaIII CATG 5 cut(s) 74, 223, 369, 430, 752
NlaIV GGNNCC 1 cut(s) 126
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 3 cut(s) 214, 228, 505
NspI RCATGY 1 cut(s) 369
PaeI GCATGC 1 cut(s) 369
PaeR7I CTCGAG 1 cut(s) 462
PagI TCATGA 1 cut(s) 426
PfeI GAWTC 2 cut(s) 524, 896
PkrI GCNGC 6 cut(s) 148, 151, 261, 264, 267, 700
PleI GAGTC 1 cut(s) 502
PluTI GGCGCC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 502
PpuMI RGGWCCY 1 cut(s) 458
PshBI ATTAAT 1 cut(s) 918
Psp5II RGGWCCY 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 931
PspGI CCWGG 1 cut(s) 931
PspN4I GGNNCC 1 cut(s) 126
PspPI GGNCC 4 cut(s) 84, 200, 458, 553
PspPPI RGGWCCY 1 cut(s) 458
PspXI VCTCGAGB 1 cut(s) 462
RsaI GTAC 2 cut(s) 335, 538
RsaNI GTAC 2 cut(s) 334, 537
SaqAI TTAA 2 cut(s) 408, 918
SatI GCNGC 6 cut(s) 147, 150, 260, 263, 266, 699
Sau3AI GATC 4 cut(s) 47, 110, 273, 620
Sau96I GGNCC 4 cut(s) 84, 200, 458, 553
SchI GAGTC 1 cut(s) 502
ScrFI CCNGG 1 cut(s) 933
SduI GDGCHC 1 cut(s) 480
SfaNI GCATC 1 cut(s) 43
SfoI GGCGCC 1 cut(s) 126
Sfr274I CTCGAG 1 cut(s) 462
SgrAI CRCCGGYG 1 cut(s) 268
SinI GGWCC 3 cut(s) 84, 200, 458
SlaI CTCGAG 1 cut(s) 462
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
SphI GCATGC 1 cut(s) 369
Sse9I AATT 3 cut(s) 346, 382, 874
SsiI CCGC 8 cut(s) 137, 146, 149, 257, 260, 263, 266, 287
SspDI GGCGCC 1 cut(s) 124
StyD4I CCNGG 1 cut(s) 931
StyI CCWWGG 3 cut(s) 80, 188, 219
TaaI ACNGT 2 cut(s) 40, 406
TaqI TCGA 4 cut(s) 276, 463, 561, 821
TasI AATT 3 cut(s) 346, 382, 874
TauI GCSGC 5 cut(s) 149, 152, 262, 265, 268
TfiI GAWTC 2 cut(s) 524, 896
Tru1I TTAA 2 cut(s) 408, 918
Tru9I TTAA 2 cut(s) 408, 918
TscAI CASTG 3 cut(s) 324, 409, 891
TseFI GTSAC 3 cut(s) 214, 228, 505
TseI GCWGC 1 cut(s) 698
Tsp45I GTSAC 3 cut(s) 214, 228, 505
TspDTI ATGAA 4 cut(s) 182, 446, 573, 612
TspGWI ACGGA 1 cut(s) 854
TspRI CASTG 3 cut(s) 324, 409, 891
VpaK11BI GGWCC 3 cut(s) 84, 200, 458
VspI ATTAAT 1 cut(s) 918
XapI RAATTY 1 cut(s) 346
XceI RCATGY 1 cut(s) 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 337
XhoI CTCGAG 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.