Prupe.5G081500_v2.0.a1

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0013944
Physical Location & Seq
Reverse (-)
9472759 .. 9478136
5378 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G081500.1

Sequence Viewer

Length: 957 bp
ATGGAGGGTAGGGAAGGAGTGAATAGTAGTGGGGTTACAGTGGTGGCATCAGATGCTCCGTCAGACTACCATGTGGCTCCAAGGTCTGAAAACACAACCCATAACGCCGGATCAACACCTCCGGCGCCGGTTGCTCCGCCACCGGCGGCGGCTCTGCCGGCGGCTGCGTCTTTGCCGATGAAGAAGAAGAGGGGGAGGCCAAGGAAGTACGGACCAGACGGGAGTGTGACCATGGCGCTTTCACCAAAGCCGATATCGTCCTCTGCGCCGCCTCCGGTGATTGATTTCTCGGCGGAGAAGCGCGGGAAAGTAAAGCCGACCAGCTCAGTGAGCAAGACCAAGTATGAGGTGGAGAATTTAGGTGAATGGGTTGCATGCTCTGTCGGTGCTAATTTTACACCACATATCATCACTGTTAATTCGGGCGAGGATGTCATGATGAAGATTATATCATTTTCTCAACAAGGTCCTCGAGCTATATGTGTGCTCTCTGCAAATGGTGTTATATCAAGCGTGACACTTCGTCAGCCTGATTCGTCTGGTGGTACATTGACATATGAGGGGCGTTTTGAGATACTATCTTTATCCGGTTCATTTATGCCCAATGAAACTGGAGGGACAAGAAGCAGATCAGGTGGGATGAGCGTTTCTTTGGCAAGTCCAGATGGGCGTGTTGTAGGTGGTGGTGTAGCTGGTCTGTTGGTAGCTGCAAGTCCTGTGCAGGTTGTAGTAGGCAGTTTTCTGTCAGGAAACCAGCATGAGCAGAAGCCCAAGAAACAGAAACATGATTACATCTCTAATGCAACGCCAACTATGGCTGTTCCTATTTCTAGTGTTGATCCAAAACCAAACTTCTCATCTTCGACTTCCTTCCGTGGAGATAATTGGTCTTCATTGCCATCGGATCCGAAAACCAAGACTGACATTAATGTATCTTTGCCTGGAGGTGTAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

319

Amino Acids

32.88

Weight (kDa)

9.39

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12080
fragaria_vesca FvH4_5g00860
malus_domestica MD00G1083100.v1.1 MD06G1063800.v1.1
prunus_persica Prupe.5G081500_v2.0.a1
pyrus_communis pycom04g06900 pycom06g06420
rosa_chinensis RchiOBHm_Chr7g0201041
rosa_laevigata RLG00000003696
rosa_multiflora Rmu_sc0006553.1_g000014
rosa_roxburghii Rroxscaffold_3G00255570
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187500 Rh7BG190800 Rh7CG198500 Rh7DG193100
rosa_wichuraiana Rw7G016450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 712
AccB1I GGYRCC 1 cut(s) 124
AccII CGCG 1 cut(s) 303
AciI CCGC 7 cut(s) 137, 146, 149, 161, 269, 293, 303
AclWI GGATC 4 cut(s) 118, 833, 899, 912
AcsI RAATTY 1 cut(s) 355
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 2 cut(s) 209, 547
AhdI GACNNNNNGTC 1 cut(s) 522
AjnI CCWGG 1 cut(s) 940
AluBI AGCT 4 cut(s) 324, 476, 692, 707
AluI AGCT 4 cut(s) 324, 476, 692, 707
Alw21I GWGCWC 1 cut(s) 489
AlwI GGATC 4 cut(s) 118, 833, 899, 912
Ama87I CYCGRG 1 cut(s) 471
AoxI GGCC 1 cut(s) 197
ApeKI GCWGC 2 cut(s) 164, 707
ApoI RAATTY 1 cut(s) 355
AseI ATTAAT 1 cut(s) 927
AspLEI GCGC 4 cut(s) 127, 238, 268, 303
AspS9I GGNCC 2 cut(s) 212, 467
AsuHPI GGTGA 3 cut(s) 234, 289, 374
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 2 cut(s) 212, 467
BamHI GGATCC 1 cut(s) 904
BanI GGYRCC 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 882
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 2 cut(s) 151, 694
BccI CCATC 2 cut(s) 659, 907
BciT130I CCWGG 1 cut(s) 942
BfaI CTAG 1 cut(s) 831
BfoI RGCGCY 2 cut(s) 128, 239
BfuAI ACCTGC 1 cut(s) 712
BisI GCNGC 6 cut(s) 147, 150, 162, 165, 269, 708
BlsI GCNGC 6 cut(s) 148, 151, 163, 166, 270, 709
Bme1390I CCNGG 1 cut(s) 942
Bme18I GGWCC 2 cut(s) 212, 467
BmeRI GACNNNNNGTC 1 cut(s) 522
BmeT110I CYCGRG 1 cut(s) 471
BmgT120I GGNCC 2 cut(s) 212, 467
BmiI GGNNCC 3 cut(s) 78, 126, 906
BmrFI CCNGG 1 cut(s) 942
BmsI GCATC 2 cut(s) 43, 56
BpiI GAAGAC 1 cut(s) 882
BpmI CTGGAG 1 cut(s) 633
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 4 cut(s) 80, 200, 231, 874
BsaWI WCCGGW 2 cut(s) 274, 587
Bse118I RCCGGY 3 cut(s) 127, 142, 157
Bse1I ACTGG 1 cut(s) 616
Bse3DI GCAATG 1 cut(s) 893
BseBI CCWGG 1 cut(s) 942
BseDI CCNNGG 4 cut(s) 80, 200, 231, 874
BseGI GGATG 2 cut(s) 436, 645
BseMI GCAATG 1 cut(s) 893
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 1 cut(s) 616
BseXI GCAGC 2 cut(s) 151, 694
BsgI GTGCAG 1 cut(s) 740
Bsh1236I CGCG 1 cut(s) 303
BshFI GGCC 1 cut(s) 199
BshNI GGYRCC 1 cut(s) 124
BsiHKAI GWGCWC 1 cut(s) 489
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 7 cut(s) 108, 122, 128, 143, 158, 275, 588
BslFI GGGAC 1 cut(s) 631
BsmFI GGGAC 1 cut(s) 631
BsnI GGCC 1 cut(s) 199
BsoBI CYCGRG 1 cut(s) 471
Bsp1286I GDGCHC 1 cut(s) 489
Bsp143I GATC 4 cut(s) 110, 629, 838, 904
Bsp19I CCATGG 1 cut(s) 231
BspACI CCGC 7 cut(s) 137, 146, 149, 161, 269, 293, 303
BspANI GGCC 1 cut(s) 199
BspCNI CTCAG 1 cut(s) 338
BspFNI CGCG 1 cut(s) 303
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 3 cut(s) 78, 126, 906
BspMI ACCTGC 1 cut(s) 712
BspPI GGATC 4 cut(s) 118, 833, 899, 912
BspT107I GGYRCC 1 cut(s) 124
BsrDI GCAATG 1 cut(s) 893
BsrFI RCCGGY 3 cut(s) 127, 142, 157
BsrI ACTGG 1 cut(s) 616
BssAI RCCGGY 3 cut(s) 127, 142, 157
BssECI CCNNGG 4 cut(s) 80, 200, 231, 874
BssMI GATC 4 cut(s) 110, 629, 838, 904
BssNI GRCGYC 1 cut(s) 125
BssT1I CCWWGG 3 cut(s) 80, 200, 231
Bst2UI CCWGG 1 cut(s) 942
Bst4CI ACNGT 2 cut(s) 40, 415
Bst6I CTCTTC 1 cut(s) 182
BstACI GRCGYC 1 cut(s) 125
BstAPI GCANNNNNTGC 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 159, 376
BstDEI CTNAG 1 cut(s) 325
BstDSI CCRYGG 2 cut(s) 231, 874
BstF5I GGATG 2 cut(s) 436, 645
BstFNI CGCG 1 cut(s) 303
BstH2I RGCGCY 2 cut(s) 128, 239
BstHHI GCGC 4 cut(s) 127, 238, 268, 303
BstKTI GATC 4 cut(s) 113, 632, 841, 907
BstMBI GATC 4 cut(s) 110, 629, 838, 904
BstMWI GCNNNNNNNGC 4 cut(s) 53, 131, 158, 330
BstNI CCWGG 1 cut(s) 942
BstNSI RCATGY 1 cut(s) 378
BstSCI CCNGG 1 cut(s) 940
BstUI CGCG 1 cut(s) 303
BstV1I GCAGC 2 cut(s) 151, 694
BstV2I GAAGAC 1 cut(s) 882
BstX2I RGATCY 1 cut(s) 904
BstYI RGATCY 1 cut(s) 904
BsuRI GGCC 1 cut(s) 199
BtgI CCRYGG 2 cut(s) 231, 874
BtsCI GGATG 2 cut(s) 436, 645
BtsIMutI CAGTG 3 cut(s) 45, 333, 411
BveI ACCTGC 1 cut(s) 712
Cac8I GCNNGC 2 cut(s) 159, 376
CciI TCATGA 1 cut(s) 435
CfoI GCGC 4 cut(s) 127, 238, 268, 303
Cfr10I RCCGGY 3 cut(s) 127, 142, 157
Cfr13I GGNCC 2 cut(s) 212, 467
CseI GACGC 1 cut(s) 156
Csp6I GTAC 2 cut(s) 208, 546
CviAII CATG 6 cut(s) 71, 232, 375, 436, 758, 785
CviQI GTAC 2 cut(s) 208, 546
DdeI CTNAG 1 cut(s) 325
DinI GGCGCC 1 cut(s) 126
DpnI GATC 4 cut(s) 112, 631, 840, 906
DpnII GATC 4 cut(s) 110, 629, 838, 904
DriI GACNNNNNGTC 1 cut(s) 522
Eam1104I CTCTTC 1 cut(s) 182
Eam1105I GACNNNNNGTC 1 cut(s) 522
EarI CTCTTC 1 cut(s) 182
EciI GGCGGA 2 cut(s) 126, 308
Eco130I CCWWGG 3 cut(s) 80, 200, 231
Eco32I GATATC 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 212, 467
Eco88I CYCGRG 1 cut(s) 471
EcoO109I RGGNCCY 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 940
EcoRV GATATC 1 cut(s) 255
EcoT14I CCWWGG 3 cut(s) 80, 200, 231
EgeI GGCGCC 1 cut(s) 126
EheI GGCGCC 1 cut(s) 126
ErhI CCWWGG 3 cut(s) 80, 200, 231
FaeI CATG 6 cut(s) 74, 235, 378, 439, 761, 788
FaqI GGGAC 1 cut(s) 631
FatI CATG 6 cut(s) 70, 231, 374, 435, 757, 784
FauI CCCGC 1 cut(s) 296
FauNDI CATATG 1 cut(s) 556
Fnu4HI GCNGC 6 cut(s) 147, 150, 162, 165, 269, 708
FokI GGATG 2 cut(s) 443, 652
Fsp4HI GCNGC 6 cut(s) 147, 150, 162, 165, 269, 708
FspBI CTAG 1 cut(s) 831
GlaI GCGC 4 cut(s) 126, 237, 267, 302
GluI GCNGC 6 cut(s) 147, 150, 162, 165, 269, 708
GsuI CTGGAG 1 cut(s) 633
HaeII RGCGCY 2 cut(s) 128, 239
HaeIII GGCC 1 cut(s) 199
HapII CCGG 7 cut(s) 108, 122, 128, 143, 158, 275, 588
HgaI GACGC 1 cut(s) 156
HhaI GCGC 4 cut(s) 127, 238, 268, 303
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 6 cut(s) 74, 235, 378, 439, 761, 788
Hin6I GCGC 4 cut(s) 125, 236, 266, 301
HinP1I GCGC 4 cut(s) 125, 236, 266, 301
HinfI GANTC 1 cut(s) 533
HpaII CCGG 7 cut(s) 108, 122, 128, 143, 158, 275, 588
HphI GGTGA 3 cut(s) 234, 289, 374
Hpy188I TCNGA 6 cut(s) 52, 64, 88, 904, 909, 956
Hpy188III TCNNGA 3 cut(s) 436, 662, 747
HpyAV CCTTC 2 cut(s) 8, 880
HpyCH4III ACNGT 2 cut(s) 40, 415
HpyCH4V TGCA 5 cut(s) 374, 494, 710, 721, 803
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 131, 158, 330
HpyF3I CTNAG 1 cut(s) 325
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 6 cut(s) 74, 235, 378, 439, 761, 788
HspAI GCGC 4 cut(s) 125, 236, 266, 301
KasI GGCGCC 1 cut(s) 124
KroI GCCGGC 1 cut(s) 157
KroNI GCCGGC 1 cut(s) 159
Kzo9I GATC 4 cut(s) 110, 629, 838, 904
LmnI GCTCC 3 cut(s) 61, 82, 139
Lsp1109I GCAGC 2 cut(s) 151, 694
LweI GCATC 2 cut(s) 43, 56
MaeI CTAG 1 cut(s) 831
MaeIII GTNAC 3 cut(s) 34, 226, 514
MalI GATC 4 cut(s) 112, 631, 840, 906
MboI GATC 4 cut(s) 110, 629, 838, 904
MboII GAAGA 6 cut(s) 193, 196, 199, 454, 852, 882
MflI RGATCY 1 cut(s) 904
MhlI GDGCHC 1 cut(s) 489
MluCI AATT 4 cut(s) 355, 391, 418, 883
Mly113I GGCGCC 1 cut(s) 125
MroNI GCCGGC 1 cut(s) 157
MseI TTAA 2 cut(s) 417, 927
MspI CCGG 7 cut(s) 108, 122, 128, 143, 158, 275, 588
MspR9I CCNGG 1 cut(s) 942
MvaI CCWGG 1 cut(s) 942
MvnI CGCG 1 cut(s) 303
MwoI GCNNNNNNNGC 4 cut(s) 53, 131, 158, 330
NaeI GCCGGC 1 cut(s) 159
NarI GGCGCC 1 cut(s) 125
NcoI CCATGG 1 cut(s) 231
NdeI CATATG 1 cut(s) 556
NdeII GATC 4 cut(s) 110, 629, 838, 904
NgoMIV GCCGGC 1 cut(s) 157
NlaIII CATG 6 cut(s) 74, 235, 378, 439, 761, 788
NlaIV GGNNCC 3 cut(s) 78, 126, 906
NmeAIII GCCGAG 1 cut(s) 269
NmuCI GTSAC 2 cut(s) 226, 514
NspI RCATGY 1 cut(s) 378
PaeI GCATGC 1 cut(s) 378
PaeR7I CTCGAG 1 cut(s) 471
PagI TCATGA 1 cut(s) 435
PdiI GCCGGC 1 cut(s) 159
PfeI GAWTC 1 cut(s) 533
PkrI GCNGC 6 cut(s) 148, 151, 163, 166, 270, 709
PluTI GGCGCC 1 cut(s) 128
PpuMI RGGWCCY 1 cut(s) 467
PshBI ATTAAT 1 cut(s) 927
Psp5II RGGWCCY 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 940
PspGI CCWGG 1 cut(s) 940
PspN4I GGNNCC 3 cut(s) 78, 126, 906
PspPI GGNCC 2 cut(s) 212, 467
PspPPI RGGWCCY 1 cut(s) 467
PspXI VCTCGAGB 1 cut(s) 471
PsuI RGATCY 1 cut(s) 904
RsaI GTAC 2 cut(s) 209, 547
RsaNI GTAC 2 cut(s) 208, 546
SaqAI TTAA 2 cut(s) 417, 927
SatI GCNGC 6 cut(s) 147, 150, 162, 165, 269, 708
Sau3AI GATC 4 cut(s) 110, 629, 838, 904
Sau96I GGNCC 2 cut(s) 212, 467
ScrFI CCNGG 1 cut(s) 942
SduI GDGCHC 1 cut(s) 489
SfaNI GCATC 2 cut(s) 43, 56
SfoI GGCGCC 1 cut(s) 126
Sfr274I CTCGAG 1 cut(s) 471
SgrAI CRCCGGYG 1 cut(s) 142
SinI GGWCC 2 cut(s) 212, 467
SlaI CTCGAG 1 cut(s) 471
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
SphI GCATGC 1 cut(s) 378
Sse9I AATT 4 cut(s) 355, 391, 418, 883
SsiI CCGC 7 cut(s) 137, 146, 149, 161, 269, 293, 303
SspDI GGCGCC 1 cut(s) 124
SspMI CTAG 1 cut(s) 831
StyD4I CCNGG 1 cut(s) 940
StyI CCWWGG 3 cut(s) 80, 200, 231
TaaI ACNGT 2 cut(s) 40, 415
TaqI TCGA 2 cut(s) 472, 863
TasI AATT 4 cut(s) 355, 391, 418, 883
TauI GCSGC 4 cut(s) 149, 152, 164, 271
TfiI GAWTC 1 cut(s) 533
Tru1I TTAA 2 cut(s) 417, 927
Tru9I TTAA 2 cut(s) 417, 927
TscAI CASTG 3 cut(s) 45, 333, 418
TseFI GTSAC 2 cut(s) 226, 514
TseI GCWGC 2 cut(s) 164, 707
Tsp45I GTSAC 2 cut(s) 226, 514
TspDTI ATGAA 5 cut(s) 194, 455, 582, 621, 882
TspGWI ACGGA 3 cut(s) 48, 225, 863
TspRI CASTG 3 cut(s) 45, 333, 418
VpaK11BI GGWCC 2 cut(s) 212, 467
VspI ATTAAT 1 cut(s) 927
XapI RAATTY 1 cut(s) 355
XceI RCATGY 1 cut(s) 378
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XhoI CTCGAG 1 cut(s) 471
XspI CTAG 1 cut(s) 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.