MD00G1083100.v1.1

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0013944
Physical Location & Seq
Forward (+)
16578647 .. 16583950
5304 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1083100.v1.1.491

Sequence Viewer

Length: 972 bp
ATGGAGGGTAGGGAAGGTGGTGTGAATAGTAGTGGGGTTACAGTTGTGGGATCAGATGCTCCATCAGACTACCATGTCGCTCCAAGGTCAGAAAACCCGACCCACAACGCTGGATCAACACCTCATGTGCCGGTTGCGCCGCCACAGGCGGCGTCTCTGCCGGCGGCAGGGTCTATGCCGATGAAGAAGAAGAGAGGGAGGCCAAGGAAGTATGGACCGGACGGGAGTGTCACCATGGCTCTGTCACCGAAGCCCATATCGTCCTCTGCGCCGCCGCCGGTGATCGATTTCTCGGCGGAGAAGCGTGGGAAAGTGAAGCCACCCAGCTCAGTGAGCAAGACCAAGTACGAGGTGGAGAATTTAGGTTTCTCAGAATGTGAATGGGTTGCATGCTCGGTTGGTGCCAATTTTACACCCCATATTATCACTGTTAATTCGGGAGAGGATGTCATGATGAAGATTATATCATTTTCTCAACAAGGTCCTCGAGCAATATGCGTGCTCTCTGCCAATGGTGTTATTTCAAGTGTGACTCTTCGTCAGCCTGATTCTTCTGGCGGTACATTGACATACGAGGGCCGTTTCGAGATATTGTCTTTATCGGGTTCATTTATGCCCAATGAAACAGGAGGGACAAGAAGCAGATCAGGCGGGATGAGTGTTTCTTTAGCAAGTCCAGATGGGCGTGTTGTAGGTGGTGGAGTTGGTGGTCTGTTAGTAGCTGCAAGTCCAGTGCAGGTTGTAGTAGGCAGTTTTCTATCAGGAAACCAGCATGAACAGAAGCCCAAGAAACAGAAACATGATTACATGGCTAATGCAACACCAACTATGGCTGTTCCTATTTCAAGTGCCGACCCAAAATCAAACTTCTCATCCTCGACTGCCTTCCGTGGAGATAATTGGTCGTCATTGGCATCAGATCCAAAAAACAAGACTGACATTAATGTATCTTTGCCGGGAGGTGTAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

324

Amino Acids

33.44

Weight (kDa)

9.2

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 138 - 252 2.7e-26 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12080
fragaria_vesca FvH4_5g00860
malus_domestica MD00G1083100.v1.1 MD06G1063800.v1.1
prunus_persica Prupe.5G081500_v2.0.a1
pyrus_communis pycom04g06900 pycom06g06420
rosa_chinensis RchiOBHm_Chr7g0201041
rosa_laevigata RLG00000003696
rosa_multiflora Rmu_sc0006553.1_g000014
rosa_roxburghii Rroxscaffold_3G00255570
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187500 Rh7BG190800 Rh7CG198500 Rh7DG193100
rosa_wichuraiana Rw7G016450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 74, 227
Acc36I ACCTGC 1 cut(s) 727
AccB1I GGYRCC 1 cut(s) 401
AciI CCGC 8 cut(s) 140, 149, 164, 272, 275, 296, 558, 651
AclWI GGATC 3 cut(s) 58, 121, 914
AcsI RAATTY 1 cut(s) 358
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 2 cut(s) 347, 562
AfiI CCNNNNNNNGG 1 cut(s) 167
AgsI TTSAA 2 cut(s) 525, 846
AhdI GACNNNNNGTC 1 cut(s) 537
AluBI AGCT 2 cut(s) 327, 722
AluI AGCT 2 cut(s) 327, 722
Alw21I GWGCWC 1 cut(s) 504
Alw26I GTCTC 1 cut(s) 159
AlwI GGATC 3 cut(s) 58, 121, 914
Ama87I CYCGRG 1 cut(s) 486
AoxI GGCC 2 cut(s) 200, 577
ApeKI GCWGC 1 cut(s) 722
ApoI RAATTY 1 cut(s) 358
AseI ATTAAT 1 cut(s) 942
AspLEI GCGC 2 cut(s) 139, 271
AspS9I GGNCC 3 cut(s) 215, 482, 577
AsuC2I CCSGG 1 cut(s) 957
AsuHPI GGTGA 3 cut(s) 223, 237, 292
AvaI CYCGRG 1 cut(s) 486
AvaII GGWCC 2 cut(s) 215, 482
BanI GGYRCC 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 504
BbvI GCAGC 1 cut(s) 709
BccI CCATC 2 cut(s) 70, 674
BceAI ACGGC 1 cut(s) 564
BcgI CGANNNNNNTGC 2 cut(s) 477, 511
BcnI CCSGG 1 cut(s) 957
BcoDI GTCTC 1 cut(s) 159
BfuAI ACCTGC 1 cut(s) 727
BisI GCNGC 6 cut(s) 140, 150, 165, 272, 275, 723
BlsI GCNGC 6 cut(s) 141, 151, 166, 273, 276, 724
Bme1390I CCNGG 1 cut(s) 957
Bme18I GGWCC 2 cut(s) 215, 482
BmeRI GACNNNNNGTC 1 cut(s) 537
BmeT110I CYCGRG 1 cut(s) 486
BmgT120I GGNCC 3 cut(s) 215, 482, 577
BmiI GGNNCC 1 cut(s) 403
BmrFI CCNGG 1 cut(s) 957
BmsI GCATC 2 cut(s) 46, 923
BpuMI CCSGG 1 cut(s) 957
Bsa29I ATCGAT 1 cut(s) 285
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 4 cut(s) 83, 203, 234, 889
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 1 cut(s) 167
Bse118I RCCGGY 3 cut(s) 130, 160, 277
Bse1I ACTGG 1 cut(s) 731
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 4 cut(s) 83, 203, 234, 889
BseGI GGATG 3 cut(s) 451, 660, 872
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMII CTCAG 2 cut(s) 342, 384
BseNI ACTGG 1 cut(s) 731
BseXI GCAGC 1 cut(s) 709
BseYI CCCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 755
BshFI GGCC 2 cut(s) 202, 579
BshNI GGYRCC 1 cut(s) 401
BshVI ATCGAT 1 cut(s) 285
BsiHKAI GWGCWC 1 cut(s) 504
BsiHKCI CYCGRG 1 cut(s) 486
BsiSI CCGG 5 cut(s) 131, 161, 218, 278, 956
BslFI GGGAC 1 cut(s) 646
BslI CCNNNNNNNGG 1 cut(s) 167
BsmAI GTCTC 1 cut(s) 159
BsmBI CGTCTC 1 cut(s) 159
BsmFI GGGAC 1 cut(s) 646
BsnI GGCC 2 cut(s) 202, 579
BsoBI CYCGRG 1 cut(s) 486
Bsp1286I GDGCHC 1 cut(s) 504
Bsp143I GATC 5 cut(s) 50, 113, 282, 644, 919
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 8 cut(s) 140, 149, 164, 272, 275, 296, 558, 651
BspANI GGCC 2 cut(s) 202, 579
BspCNI CTCAG 2 cut(s) 341, 383
BspDI ATCGAT 1 cut(s) 285
BspHI TCATGA 1 cut(s) 450
BspLI GGNNCC 1 cut(s) 403
BspMI ACCTGC 1 cut(s) 727
BspPI GGATC 3 cut(s) 58, 121, 914
BspT107I GGYRCC 1 cut(s) 401
BsrFI RCCGGY 3 cut(s) 130, 160, 277
BsrI ACTGG 1 cut(s) 731
BssAI RCCGGY 3 cut(s) 130, 160, 277
BssECI CCNNGG 4 cut(s) 83, 203, 234, 889
BssMI GATC 5 cut(s) 50, 113, 282, 644, 919
BssNI GRCGYC 1 cut(s) 152
BssT1I CCWWGG 3 cut(s) 83, 203, 234
Bst4CI ACNGT 2 cut(s) 43, 430
Bst6I CTCTTC 2 cut(s) 185, 540
BstACI GRCGYC 1 cut(s) 152
BstC8I GCNNGC 3 cut(s) 162, 391, 500
BstDEI CTNAG 2 cut(s) 328, 370
BstDSI CCRYGG 2 cut(s) 234, 889
BstF5I GGATG 3 cut(s) 451, 660, 872
BstHHI GCGC 2 cut(s) 139, 271
BstKTI GATC 5 cut(s) 53, 116, 285, 647, 922
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 5 cut(s) 50, 113, 282, 644, 919
BstMWI GCNNNNNNNGC 3 cut(s) 136, 333, 648
BstNSI RCATGY 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 955
BstV1I GCAGC 1 cut(s) 709
BstX2I RGATCY 1 cut(s) 919
BstXI CCANNNNNNTGG 1 cut(s) 110
BstYI RGATCY 1 cut(s) 919
Bsu15I ATCGAT 1 cut(s) 285
BsuRI GGCC 2 cut(s) 202, 579
BsuTUI ATCGAT 1 cut(s) 285
BtgI CCRYGG 2 cut(s) 234, 889
BtsCI GGATG 3 cut(s) 451, 660, 872
BtsIMutI CAGTG 3 cut(s) 336, 426, 738
BveI ACCTGC 1 cut(s) 727
Cac8I GCNNGC 3 cut(s) 162, 391, 500
CciI TCATGA 1 cut(s) 450
CfoI GCGC 2 cut(s) 139, 271
Cfr10I RCCGGY 3 cut(s) 130, 160, 277
Cfr13I GGNCC 3 cut(s) 215, 482, 577
ClaI ATCGAT 1 cut(s) 285
CseI GACGC 1 cut(s) 141
Csp6I GTAC 2 cut(s) 346, 561
CviAII CATG 8 cut(s) 74, 125, 235, 390, 451, 773, 800, 808
CviQI GTAC 2 cut(s) 346, 561
DdeI CTNAG 2 cut(s) 328, 370
DpnI GATC 5 cut(s) 52, 115, 284, 646, 921
DpnII GATC 5 cut(s) 50, 113, 282, 644, 919
DrdI GACNNNNNNGTC 2 cut(s) 74, 227
DriI GACNNNNNGTC 1 cut(s) 537
DseDI GACNNNNNNGTC 2 cut(s) 74, 227
Eam1104I CTCTTC 2 cut(s) 185, 540
Eam1105I GACNNNNNGTC 1 cut(s) 537
EarI CTCTTC 2 cut(s) 185, 540
EciI GGCGGA 1 cut(s) 311
Eco130I CCWWGG 3 cut(s) 83, 203, 234
Eco47I GGWCC 2 cut(s) 215, 482
Eco88I CYCGRG 1 cut(s) 486
EcoO109I RGGNCCY 1 cut(s) 482
EcoT14I CCWWGG 3 cut(s) 83, 203, 234
ErhI CCWWGG 3 cut(s) 83, 203, 234
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 8 cut(s) 77, 128, 238, 393, 454, 776, 803, 811
FaqI GGGAC 1 cut(s) 646
FatI CATG 8 cut(s) 73, 124, 234, 389, 450, 772, 799, 807
FauI CCCGC 1 cut(s) 644
Fnu4HI GCNGC 6 cut(s) 140, 150, 165, 272, 275, 723
FokI GGATG 3 cut(s) 458, 667, 859
Fsp4HI GCNGC 6 cut(s) 140, 150, 165, 272, 275, 723
GlaI GCGC 2 cut(s) 138, 270
GluI GCNGC 6 cut(s) 140, 150, 165, 272, 275, 723
GsaI CCCAGC 1 cut(s) 327
HaeIII GGCC 2 cut(s) 202, 579
HapII CCGG 5 cut(s) 131, 161, 218, 278, 956
HgaI GACGC 1 cut(s) 141
HhaI GCGC 2 cut(s) 139, 271
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 8 cut(s) 77, 128, 238, 393, 454, 776, 803, 811
Hin6I GCGC 2 cut(s) 137, 269
HinP1I GCGC 2 cut(s) 137, 269
HinfI GANTC 2 cut(s) 532, 548
HpaII CCGG 5 cut(s) 131, 161, 218, 278, 956
HphI GGTGA 3 cut(s) 223, 237, 292
Hpy188I TCNGA 5 cut(s) 55, 67, 91, 373, 919
Hpy188III TCNNGA 5 cut(s) 438, 451, 586, 677, 762
HpyAV CCTTC 2 cut(s) 8, 895
HpyCH4III ACNGT 2 cut(s) 43, 430
HpyCH4V TGCA 4 cut(s) 389, 725, 736, 818
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 333, 648
HpyF3I CTNAG 2 cut(s) 328, 370
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 8 cut(s) 77, 128, 238, 393, 454, 776, 803, 811
HspAI GCGC 2 cut(s) 137, 269
KroI GCCGGC 1 cut(s) 160
KroNI GCCGGC 1 cut(s) 162
Kzo9I GATC 5 cut(s) 50, 113, 282, 644, 919
LmnI GCTCC 2 cut(s) 64, 85
Lsp1109I GCAGC 1 cut(s) 709
LweI GCATC 2 cut(s) 46, 923
MaeIII GTNAC 4 cut(s) 37, 229, 243, 529
MalI GATC 5 cut(s) 52, 115, 284, 646, 921
MboI GATC 5 cut(s) 50, 113, 282, 644, 919
MboII GAAGA 6 cut(s) 196, 199, 202, 469, 527, 543
MflI RGATCY 1 cut(s) 919
MhlI GDGCHC 1 cut(s) 504
MluCI AATT 4 cut(s) 358, 406, 433, 898
MlyI GAGTC 1 cut(s) 526
MroNI GCCGGC 1 cut(s) 160
MseI TTAA 2 cut(s) 432, 942
MspI CCGG 5 cut(s) 131, 161, 218, 278, 956
MspR9I CCNGG 1 cut(s) 957
MwoI GCNNNNNNNGC 3 cut(s) 136, 333, 648
NaeI GCCGGC 1 cut(s) 162
NciI CCSGG 1 cut(s) 957
NcoI CCATGG 1 cut(s) 234
NdeII GATC 5 cut(s) 50, 113, 282, 644, 919
NgoMIV GCCGGC 1 cut(s) 160
NlaIII CATG 8 cut(s) 77, 128, 238, 393, 454, 776, 803, 811
NlaIV GGNNCC 1 cut(s) 403
NmeAIII GCCGAG 1 cut(s) 272
NmuCI GTSAC 3 cut(s) 229, 243, 529
NspI RCATGY 1 cut(s) 393
PaeI GCATGC 1 cut(s) 393
PaeR7I CTCGAG 1 cut(s) 486
PagI TCATGA 1 cut(s) 450
PdiI GCCGGC 1 cut(s) 162
PfeI GAWTC 1 cut(s) 548
PkrI GCNGC 6 cut(s) 141, 151, 166, 273, 276, 724
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PpuMI RGGWCCY 1 cut(s) 482
PshBI ATTAAT 1 cut(s) 942
Psp5II RGGWCCY 1 cut(s) 482
PspFI CCCAGC 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 403
PspPI GGNCC 3 cut(s) 215, 482, 577
PspPPI RGGWCCY 1 cut(s) 482
PspXI VCTCGAGB 1 cut(s) 486
PsuI RGATCY 1 cut(s) 919
RsaI GTAC 2 cut(s) 347, 562
RsaNI GTAC 2 cut(s) 346, 561
SaqAI TTAA 2 cut(s) 432, 942
SatI GCNGC 6 cut(s) 140, 150, 165, 272, 275, 723
Sau3AI GATC 5 cut(s) 50, 113, 282, 644, 919
Sau96I GGNCC 3 cut(s) 215, 482, 577
SchI GAGTC 1 cut(s) 526
ScrFI CCNGG 1 cut(s) 957
SduI GDGCHC 1 cut(s) 504
SfaNI GCATC 2 cut(s) 46, 923
Sfr274I CTCGAG 1 cut(s) 486
SgrAI CRCCGGYG 1 cut(s) 277
SinI GGWCC 2 cut(s) 215, 482
SlaI CTCGAG 1 cut(s) 486
SmlI CTYRAG 1 cut(s) 486
SmoI CTYRAG 1 cut(s) 486
SphI GCATGC 1 cut(s) 393
Sse9I AATT 4 cut(s) 358, 406, 433, 898
SsiI CCGC 8 cut(s) 140, 149, 164, 272, 275, 296, 558, 651
StyD4I CCNGG 1 cut(s) 955
StyI CCWWGG 3 cut(s) 83, 203, 234
TaaI ACNGT 2 cut(s) 43, 430
TaqI TCGA 4 cut(s) 285, 487, 585, 878
TasI AATT 4 cut(s) 358, 406, 433, 898
TauI GCSGC 5 cut(s) 142, 152, 167, 274, 277
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 2 cut(s) 432, 942
Tru9I TTAA 2 cut(s) 432, 942
TscAI CASTG 3 cut(s) 336, 433, 738
TseFI GTSAC 3 cut(s) 229, 243, 529
TseI GCWGC 1 cut(s) 722
Tsp45I GTSAC 3 cut(s) 229, 243, 529
TspDTI ATGAA 5 cut(s) 197, 470, 597, 636, 789
TspGWI ACGGA 1 cut(s) 878
TspRI CASTG 3 cut(s) 336, 433, 738
VpaK11BI GGWCC 2 cut(s) 215, 482
VspI ATTAAT 1 cut(s) 942
XapI RAATTY 1 cut(s) 358
XceI RCATGY 1 cut(s) 393
XcmI CCANNNNNNNNNTGG 1 cut(s) 349
XhoI CTCGAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.