FvH4_5g01944

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007441
Physical Location & Seq
Reverse (-)
1222719 .. 1226840
4122 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01944.t2

Sequence Viewer

Length: 750 bp
ATGGATTTGCTCTTAGCAATTGCTGTGGTAGGTTTCGGAGTATGGATGAGCACTCATCATGATGGCTGTCGGAAATCGCTCACAATCCCTGTTTTAGCAATTGGTGCTGTCATCTTTTTAGTATCTATAATCGGGTTCTTGGGTGCGCTGAAAAGAAGCTCCATACTGTTGTGGATTTATTTGATTCTCCTGTGCTTTATATTGGTGGGCATCCTGGTGTTCACCGTATTAGCGTTCATTGTCACAAATAATGGTTCTGGTCATAGTGTTGCAGACTTGAGGTTCAAGGAGTATCGACTCAAGGATTACAGTTCATGGTTCCTAAAAGAATTGAACAATACCCATAACTGGAAGCACTTAAAAAGTTGTCTTGTGAAATCAGATGATTGCAATAACCTCTCAAAGAAGTATAAGACTCTCAAGAAATTCAAATTGGCAAAGCTGACCCCTGTTGAGGCTGGTTGCTGTCGACCTCCATCTGAATGCGGTTATCCAGCTGTTAATGCCTCGTACTATGACTTGAGCTTTCATCCAGTTAGTTCAAACAAGGACTGCAAACTTTACAAAAATGCTCGGGACACCAAGTGCTATAATTGTGATTCCTGCAAGGCTGGAGTTGCACAATACATGAAAACTGAGTGGAGAGTGGTTGCAATTTTCAACGTTGCTCTCTTTGTTGTTTTGTCTCTGATATACTTTGTGGGCTGTTGTGCGAGACGAAATGCTGCAAGACACCGTTCTAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

250

Amino Acids

28.0

Weight (kDa)

9.46

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 8 - 253 3.3e-36 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 469
AciI CCGC 1 cut(s) 486
AclI AACGTT 1 cut(s) 663
AcsI RAATTY 1 cut(s) 425
AdeI CACNNNGTG 1 cut(s) 585
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 2 cut(s) 348, 454
AgsI TTSAA 5 cut(s) 286, 334, 430, 543, 661
AjnI CCWGG 1 cut(s) 213
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AluBI AGCT 4 cut(s) 159, 442, 497, 525
AluI AGCT 4 cut(s) 159, 442, 497, 525
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 2 cut(s) 690, 709
Ama87I CYCGRG 1 cut(s) 573
ApeKI GCWGC 1 cut(s) 725
ApoI RAATTY 1 cut(s) 425
AspLEI GCGC 1 cut(s) 148
AsuHPI GGTGA 1 cut(s) 214
AvaI CYCGRG 1 cut(s) 573
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 712
BccI CCATC 2 cut(s) 56, 484
BciT130I CCWGG 1 cut(s) 215
BcoDI GTCTC 2 cut(s) 690, 709
BisI GCNGC 1 cut(s) 726
BlsI GCNGC 1 cut(s) 727
Bme1390I CCNGG 1 cut(s) 215
BmeT110I CYCGRG 1 cut(s) 573
BmiI GGNNCC 1 cut(s) 320
BmrFI CCNGG 1 cut(s) 215
BmsI GCATC 1 cut(s) 219
BpmI CTGGAG 1 cut(s) 633
BpuEI CTTGAG 4 cut(s) 284, 298, 404, 541
Bsc4I CCNNNNNNNGG 2 cut(s) 348, 454
Bse1I ACTGG 2 cut(s) 353, 533
BseBI CCWGG 1 cut(s) 215
BseGI GGATG 3 cut(s) 51, 210, 529
BseLI CCNNNNNNNGG 2 cut(s) 348, 454
BseMII CTCAG 1 cut(s) 627
BseNI ACTGG 2 cut(s) 353, 533
BseXI GCAGC 1 cut(s) 712
BsiHKAI GWGCWC 1 cut(s) 53
BsiHKCI CYCGRG 1 cut(s) 573
BslFI GGGAC 1 cut(s) 590
BslI CCNNNNNNNGG 2 cut(s) 348, 454
BsmAI GTCTC 2 cut(s) 690, 709
BsmBI CGTCTC 1 cut(s) 709
BsmFI GGGAC 1 cut(s) 590
BsmI GAATGC 1 cut(s) 488
BsoBI CYCGRG 1 cut(s) 573
Bsp1286I GDGCHC 1 cut(s) 53
BspACI CCGC 1 cut(s) 486
BspCNI CTCAG 1 cut(s) 628
BspHI TCATGA 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 320
BsrI ACTGG 2 cut(s) 353, 533
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 4 cut(s) 168, 226, 311, 737
BstAPI GCANNNNNTGC 1 cut(s) 104
BstDEI CTNAG 2 cut(s) 13, 636
BstF5I GGATG 3 cut(s) 51, 210, 529
BstHHI GCGC 1 cut(s) 148
BstMAI GTCTC 2 cut(s) 690, 709
BstMWI GCNNNNNNNGC 3 cut(s) 104, 503, 617
BstNI CCWGG 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 213
BstV1I GCAGC 1 cut(s) 712
BtsCI GGATG 3 cut(s) 51, 210, 529
CciI TCATGA 1 cut(s) 58
CfoI GCGC 1 cut(s) 148
Csp6I GTAC 1 cut(s) 511
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 3 cut(s) 59, 315, 628
CviJI RGCY 8 cut(s) 66, 159, 442, 458, 497, 525, 611, 705
CviKI_1 RGCY 8 cut(s) 66, 159, 442, 458, 497, 525, 611, 705
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 2 cut(s) 13, 636
DraIII CACNNNGTG 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 573
EcoRII CCWGG 1 cut(s) 213
Esp3I CGTCTC 1 cut(s) 709
FaeI CATG 3 cut(s) 62, 318, 631
FaqI GGGAC 1 cut(s) 590
FatI CATG 3 cut(s) 58, 314, 627
FblI GTMKAC 1 cut(s) 469
Fnu4HI GCNGC 1 cut(s) 726
FokI GGATG 3 cut(s) 58, 197, 516
Fsp4HI GCNGC 1 cut(s) 726
GlaI GCGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 726
GsuI CTGGAG 1 cut(s) 633
HhaI GCGC 1 cut(s) 148
Hin1II CATG 3 cut(s) 62, 318, 631
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HincII GTYRAC 1 cut(s) 470
HindII GTYRAC 1 cut(s) 470
HinfI GANTC 4 cut(s) 184, 297, 415, 599
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 2 cut(s) 222, 470
Hpy188I TCNGA 5 cut(s) 38, 72, 382, 481, 690
Hpy188III TCNNGA 3 cut(s) 59, 421, 575
Hpy8I GTNNAC 2 cut(s) 222, 470
HpyCH4III ACNGT 4 cut(s) 168, 226, 311, 737
HpyCH4IV ACGT 1 cut(s) 663
HpyCH4V TGCA 7 cut(s) 272, 390, 555, 606, 620, 653, 728
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 503, 617
HpyF3I CTNAG 2 cut(s) 13, 636
HpySE526I ACGT 1 cut(s) 663
Hsp92II CATG 3 cut(s) 62, 318, 631
HspAI GCGC 1 cut(s) 146
LmnI GCTCC 1 cut(s) 164
Lsp1109I GCAGC 1 cut(s) 712
LweI GCATC 1 cut(s) 219
MaeII ACGT 1 cut(s) 663
MaeIII GTNAC 1 cut(s) 241
MfeI CAATTG 2 cut(s) 18, 99
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 7 cut(s) 18, 99, 329, 425, 431, 592, 654
MlyI GAGTC 2 cut(s) 291, 409
MmeI TCCRAC 1 cut(s) 50
MnlI CCTC 5 cut(s) 273, 407, 448, 483, 517
MseI TTAA 3 cut(s) 359, 501, 748
MslI CAYNNNNRTG 3 cut(s) 60, 215, 481
MspA1I CMGCKG 1 cut(s) 497
MspR9I CCNGG 1 cut(s) 215
MunI CAATTG 2 cut(s) 18, 99
Mva1269I GAATGC 1 cut(s) 488
MvaI CCWGG 1 cut(s) 215
MwoI GCNNNNNNNGC 3 cut(s) 104, 503, 617
NlaIII CATG 3 cut(s) 62, 318, 631
NlaIV GGNNCC 1 cut(s) 320
NmuCI GTSAC 1 cut(s) 241
PagI TCATGA 1 cut(s) 58
PctI GAATGC 1 cut(s) 488
PfeI GAWTC 2 cut(s) 184, 599
PkrI GCNGC 1 cut(s) 727
PleI GAGTC 2 cut(s) 291, 409
PpsI GAGTC 2 cut(s) 291, 409
Psp1406I AACGTT 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
RseI CAYNNNNRTG 3 cut(s) 60, 215, 481
SalI GTCGAC 1 cut(s) 468
SaqAI TTAA 3 cut(s) 359, 501, 748
SatI GCNGC 1 cut(s) 726
SchI GAGTC 2 cut(s) 291, 409
ScrFI CCNGG 1 cut(s) 215
SduI GDGCHC 1 cut(s) 53
SetI ASST 9 cut(s) 34, 161, 284, 399, 444, 475, 499, 527, 666
SfaNI GCATC 1 cut(s) 219
SmiMI CAYNNNNRTG 3 cut(s) 60, 215, 481
SmlI CTYRAG 4 cut(s) 277, 299, 419, 520
SmoI CTYRAG 4 cut(s) 277, 299, 419, 520
Sse9I AATT 7 cut(s) 18, 99, 329, 425, 431, 592, 654
SsiI CCGC 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 213
TaaI ACNGT 4 cut(s) 168, 226, 311, 737
TaiI ACGT 1 cut(s) 666
TaqI TCGA 2 cut(s) 295, 469
TasI AATT 7 cut(s) 18, 99, 329, 425, 431, 592, 654
TfiI GAWTC 2 cut(s) 184, 599
Tru1I TTAA 3 cut(s) 359, 501, 748
Tru9I TTAA 3 cut(s) 359, 501, 748
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 1 cut(s) 725
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 4 cut(s) 226, 303, 518, 644
XapI RAATTY 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.