FvH4_5g05540

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012442
Physical Location & Seq
Forward (+)
3256924 .. 3259006
2083 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05540.t2

Sequence Viewer

Length: 1062 bp
ATGGATGTGGCGCATTGCCATTTAGAGGGAAACGCTGATGCCGTAGAGTTTTGTCCCCACGAATCGTATCGCCATGTTTTGGCAGCTTCAACTTACACACTGCAAGAGGGTGATCAGCCGAGCCGAGCAGGAAGCATATCGCTTTTTGATGTTGATGCTGACACGGCTCGCCTGGATTTGTGTCACCGCGTTGAAACGGCTGGAATCTTTGATATAAAGTGGAACCCAGTTGGAGAATGTGGTATTGATCCTCTTCTTGCTCTAGCTGATGCTGATGGTTACTTGAGAATTCATGGTCTTGGGTGCTCTTCAGATGAAGTGAAAGGGTGGTCTTTGAGAGAGATAGGTGATGAAAAGATCAGTTCTTCCATGTGCCTTTTCCTGGACTGGAACCCTTCAGCCACAGCTATCACAGTGGGGCTTTCGAATGGTTCTGTCTCAATAGTTTCTCTAGCTGAATCCCAACTTGAGACACAAGAAATATGGAAAGCTCATGATTTTGAGGTTTGGACAACTTCCTTTGACATCCACCAGCCACAGTTAGTATACACCGGCTCAGATGACTGCAAGTTCAAATGCTGGGATTTGAGAGATGGTCCTTCTAAGTTGGCATTTCAGAATTCCAAGGTCCATACAATGGGAGTTACTTGCATAGCCAAAAACCCTAGTGACCCCAATACTGTACTTACTGGTAGCTATGATGAGTACCTAAGGGTATGGGATGTGAGATCAATATCAAAACCAGTCAATGAAATGTCTACTTGTTTGGGAGGAGGTGTCTGGAGAATCAAGTACCATCCCTTCGTAAGCAGTCTAGTCTTGGCAGCTTGTATGCACAATGGCTTTTCTATTGTCAATATTAACGGTGGTAAAACTGAAGTCATTGAAACATATTCGAAGCATGAATCACTTGCATATGGAGCTGATTGGCATAAAGGGGTATCATTCCATGAAGGCAAAGGAAATGCCACTTTAGTCGCAACATGCTCGTTTTACGACCGGCTTCTTCAGATATGGATGCCCGAAAGTGATTTGAAATTGCACCAGGACATTGTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

354

Amino Acids

38.98

Weight (kDa)

5.04

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 117 - 273 3.7e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 126 - 245 5.9e-11 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 127 - 199 2.4e-06 THOC3 beta-propeller domain
WDR55 PF24796 128 - 302 2.9e-10 WDR55
Beta-prop_WDR36-Utp21_1st PF25171 128 - 240 6e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 130 - 302 1.7e-10 WDR5 beta-propeller domain
Beta-prop_EIPR1 PF23609 142 - 277 7.1e-08 EIPR1 beta-propeller
WD40_Prp19 PF24814 172 - 294 7.9e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 207 - 296 9.1e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012442)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63010
fragaria_vesca FvH4_5g05540 FvH4_5g05540
malus_domestica MD06G1171600.v1.1
prunus_persica Prupe.5G171000_v2.0.a1 Prupe.5G171000_v2.0.a1
pyrus_communis pycom06g15270
rosa_chinensis RchiOBHm_Chr7g0193241
rosa_laevigata RLG00000004284
rosa_multiflora Rmu_ssc0000092.1_g000021
rosa_roxburghii Rroxscaffold_3G00262260
rosa_rugosa Rorug07G0008400 Rorug07G0008400 Rorug07G0008400
rosa_samantha Rh7AG134000 Rh7BG135000 Rh7CG138000 Rh7DG136000
rosa_wichuraiana Rw7G011460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 79, 637
AccI GTMKAC 2 cut(s) 546, 758
AccII CGCG 1 cut(s) 189
AciI CCGC 1 cut(s) 187
AclWI GGATC 1 cut(s) 242
AcsI RAATTY 2 cut(s) 288, 619
AcuI CTGAAG 4 cut(s) 294, 381, 897, 992
AfaI GTAC 3 cut(s) 684, 707, 794
AfiI CCNNNNNNNGG 4 cut(s) 25, 79, 382, 637
AgsI TTSAA 5 cut(s) 90, 194, 574, 887, 1036
AjnI CCWGG 3 cut(s) 171, 381, 1044
AluBI AGCT 8 cut(s) 86, 266, 407, 455, 491, 696, 827, 923
AluI AGCT 8 cut(s) 86, 266, 407, 455, 491, 696, 827, 923
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 2 cut(s) 442, 464
AlwI GGATC 1 cut(s) 242
ApeKI GCWGC 2 cut(s) 83, 824
ApoI RAATTY 2 cut(s) 288, 619
AspLEI GCGC 1 cut(s) 13
AspS9I GGNCC 2 cut(s) 596, 628
AsuHPI GGTGA 3 cut(s) 122, 176, 359
AsuII TTCGAA 2 cut(s) 425, 896
AvaII GGWCC 2 cut(s) 596, 628
AxyI CCTNAGG 1 cut(s) 710
BarI GAAGNNNNNNTAC 2 cut(s) 785, 817
Bbv12I GWGCWC 1 cut(s) 308
BbvI GCAGC 2 cut(s) 95, 836
BccI CCATC 3 cut(s) 269, 587, 804
BceAI ACGGC 3 cut(s) 26, 180, 213
BcgI CGANNNNNNTGC 2 cut(s) 969, 1003
BciT130I CCWGG 3 cut(s) 173, 383, 1046
BclI TGATCA 1 cut(s) 112
BcoDI GTCTC 2 cut(s) 442, 464
BfaI CTAG 4 cut(s) 263, 452, 666, 815
BisI GCNGC 2 cut(s) 84, 825
BlsI GCNGC 2 cut(s) 85, 826
Bme1390I CCNGG 3 cut(s) 173, 383, 1046
Bme18I GGWCC 2 cut(s) 596, 628
BmgT120I GGNCC 2 cut(s) 596, 628
BmiI GGNNCC 2 cut(s) 224, 392
BmrFI CCNGG 3 cut(s) 173, 383, 1046
BmrI ACTGGG 1 cut(s) 221
BmsI GCATC 4 cut(s) 28, 145, 259, 1008
BmuI ACTGGG 1 cut(s) 221
BpmI CTGGAG 1 cut(s) 802
Bpu14I TTCGAA 2 cut(s) 425, 896
BpuEI CTTGAG 2 cut(s) 304, 488
BsaBI GATNNNNATC 1 cut(s) 733
BsaJI CCNNGG 1 cut(s) 624
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 79, 382, 637
Bse118I RCCGGY 2 cut(s) 551, 999
Bse1I ACTGG 4 cut(s) 227, 392, 694, 743
Bse21I CCTNAGG 1 cut(s) 710
Bse3DI GCAATG 1 cut(s) 13
Bse8I GATNNNNATC 1 cut(s) 733
BseBI CCWGG 3 cut(s) 173, 383, 1046
BseDI CCNNGG 1 cut(s) 624
BseGI GGATG 5 cut(s) 10, 525, 727, 796, 1023
BseJI GATNNNNATC 1 cut(s) 733
BseLI CCNNNNNNNGG 4 cut(s) 25, 79, 382, 637
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 4 cut(s) 227, 392, 694, 743
BseRI GAGGAG 1 cut(s) 786
BseXI GCAGC 2 cut(s) 95, 836
BseYI CCCAGC 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 189
Bsh1285I CGRYCG 1 cut(s) 1000
BsiEI CGRYCG 1 cut(s) 1000
BsiHKAI GWGCWC 1 cut(s) 308
BsiSI CCGG 2 cut(s) 552, 1000
BslFI GGGAC 1 cut(s) 39
BslI CCNNNNNNNGG 4 cut(s) 25, 79, 382, 637
BsmAI GTCTC 2 cut(s) 442, 464
BsmFI GGGAC 1 cut(s) 39
Bsp119I TTCGAA 2 cut(s) 425, 896
Bsp1286I GDGCHC 1 cut(s) 308
Bsp143I GATC 4 cut(s) 112, 247, 357, 728
BspACI CCGC 1 cut(s) 187
BspCNI CTCAG 1 cut(s) 569
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 1 cut(s) 493
BspLI GGNNCC 2 cut(s) 224, 392
BspPI GGATC 1 cut(s) 242
BspQI GCTCTTC 1 cut(s) 313
BspT104I TTCGAA 2 cut(s) 425, 896
BsrDI GCAATG 1 cut(s) 13
BsrFI RCCGGY 2 cut(s) 551, 999
BsrI ACTGG 4 cut(s) 227, 392, 694, 743
BssAI RCCGGY 2 cut(s) 551, 999
BssECI CCNNGG 1 cut(s) 624
BssMI GATC 4 cut(s) 112, 247, 357, 728
BssNAI GTATAC 1 cut(s) 547
BssT1I CCWWGG 1 cut(s) 624
Bst1107I GTATAC 1 cut(s) 547
Bst2UI CCWGG 3 cut(s) 173, 383, 1046
Bst4CI ACNGT 4 cut(s) 415, 540, 682, 866
Bst6I CTCTTC 2 cut(s) 258, 313
BstBI TTCGAA 2 cut(s) 425, 896
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 3 cut(s) 556, 603, 710
BstF5I GGATG 5 cut(s) 10, 525, 727, 796, 1023
BstFNI CGCG 1 cut(s) 189
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 4 cut(s) 115, 250, 360, 731
BstMAI GTCTC 2 cut(s) 442, 464
BstMBI GATC 4 cut(s) 112, 247, 357, 728
BstMCI CGRYCG 1 cut(s) 1000
BstMWI GCNNNNNNNGC 2 cut(s) 164, 920
BstNI CCWGG 3 cut(s) 173, 383, 1046
BstNSI RCATGY 1 cut(s) 987
BstSCI CCNGG 3 cut(s) 171, 381, 1044
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 95, 836
BstZ17I GTATAC 1 cut(s) 547
Bsu36I CCTNAGG 1 cut(s) 710
BtsCI GGATG 5 cut(s) 10, 525, 727, 796, 1023
BtsI GCAGTG 1 cut(s) 98
BtsIMutI CAGTG 2 cut(s) 98, 420
Cac8I GCNNGC 1 cut(s) 169
CciI TCATGA 1 cut(s) 493
CfoI GCGC 1 cut(s) 13
Cfr10I RCCGGY 2 cut(s) 551, 999
Cfr13I GGNCC 2 cut(s) 596, 628
Csp6I GTAC 3 cut(s) 683, 706, 793
CviAII CATG 7 cut(s) 74, 293, 370, 494, 902, 950, 984
CviQI GTAC 3 cut(s) 683, 706, 793
DdeI CTNAG 3 cut(s) 556, 603, 710
DpnI GATC 4 cut(s) 114, 249, 359, 730
DpnII GATC 4 cut(s) 112, 247, 357, 728
Eam1104I CTCTTC 2 cut(s) 258, 313
EarI CTCTTC 2 cut(s) 258, 313
Eco130I CCWWGG 1 cut(s) 624
Eco47I GGWCC 2 cut(s) 596, 628
Eco57I CTGAAG 4 cut(s) 294, 381, 897, 992
Eco81I CCTNAGG 1 cut(s) 710
EcoRI GAATTC 2 cut(s) 288, 619
EcoRII CCWGG 3 cut(s) 171, 381, 1044
EcoT14I CCWWGG 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 624
FaeI CATG 7 cut(s) 77, 296, 373, 497, 905, 953, 987
FaqI GGGAC 1 cut(s) 39
FatI CATG 7 cut(s) 73, 292, 369, 493, 901, 949, 983
FauNDI CATATG 1 cut(s) 916
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 2 cut(s) 546, 758
Fnu4HI GCNGC 2 cut(s) 84, 825
FokI GGATG 5 cut(s) 17, 512, 734, 783, 1030
Fsp4HI GCNGC 2 cut(s) 84, 825
FspBI CTAG 4 cut(s) 263, 452, 666, 815
GlaI GCGC 1 cut(s) 12
GluI GCNGC 2 cut(s) 84, 825
GsaI CCCAGC 1 cut(s) 583
GsuI CTGGAG 1 cut(s) 802
HapII CCGG 2 cut(s) 552, 1000
HhaI GCGC 1 cut(s) 13
Hin1II CATG 7 cut(s) 77, 296, 373, 497, 905, 953, 987
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HinfI GANTC 5 cut(s) 62, 204, 458, 786, 905
HpaII CCGG 2 cut(s) 552, 1000
HphI GGTGA 3 cut(s) 122, 176, 359
Hpy166II GTNNAC 2 cut(s) 547, 759
Hpy188I TCNGA 4 cut(s) 313, 559, 618, 1011
Hpy188III TCNNGA 2 cut(s) 494, 781
Hpy8I GTNNAC 2 cut(s) 547, 759
HpyAV CCTTC 4 cut(s) 405, 609, 811, 947
HpyCH4III ACNGT 4 cut(s) 415, 540, 682, 866
HpyCH4V TGCA 6 cut(s) 103, 567, 651, 835, 914, 1042
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 920
HpyF3I CTNAG 3 cut(s) 556, 603, 710
Hsp92II CATG 7 cut(s) 77, 296, 373, 497, 905, 953, 987
HspAI GCGC 1 cut(s) 11
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 4 cut(s) 112, 247, 357, 728
LguI GCTCTTC 1 cut(s) 313
LmnI GCTCC 1 cut(s) 920
Lsp1109I GCAGC 2 cut(s) 95, 836
LweI GCATC 4 cut(s) 28, 145, 259, 1008
MaeI CTAG 4 cut(s) 263, 452, 666, 815
MaeIII GTNAC 4 cut(s) 182, 278, 643, 668
MalI GATC 4 cut(s) 114, 249, 359, 730
MboI GATC 4 cut(s) 112, 247, 357, 728
MboII GAAGA 4 cut(s) 245, 300, 357, 998
MhlI GDGCHC 1 cut(s) 308
MluCI AATT 3 cut(s) 288, 619, 1037
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 6 cut(s) 19, 100, 261, 496, 764, 767
MseI TTAA 1 cut(s) 861
MspI CCGG 2 cut(s) 552, 1000
MspR9I CCNGG 3 cut(s) 173, 383, 1046
MvaI CCWGG 3 cut(s) 173, 383, 1046
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 164, 920
NdeI CATATG 1 cut(s) 916
NdeII GATC 4 cut(s) 112, 247, 357, 728
NlaIII CATG 7 cut(s) 77, 296, 373, 497, 905, 953, 987
NlaIV GGNNCC 2 cut(s) 224, 392
NmeAIII GCCGAG 2 cut(s) 144, 149
NmuCI GTSAC 2 cut(s) 182, 668
NspI RCATGY 1 cut(s) 987
NspV TTCGAA 2 cut(s) 425, 896
PagI TCATGA 1 cut(s) 493
PciSI GCTCTTC 1 cut(s) 313
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PfeI GAWTC 5 cut(s) 62, 204, 458, 786, 905
PflMI CCANNNNNTGG 2 cut(s) 79, 637
PfoI TCCNGGA 1 cut(s) 381
PkrI GCNGC 2 cut(s) 85, 826
Psp6I CCWGG 3 cut(s) 171, 381, 1044
PspFI CCCAGC 1 cut(s) 579
PspGI CCWGG 3 cut(s) 171, 381, 1044
PspN4I GGNNCC 2 cut(s) 224, 392
PspPI GGNCC 2 cut(s) 596, 628
RsaI GTAC 3 cut(s) 684, 707, 794
RsaNI GTAC 3 cut(s) 683, 706, 793
SapI GCTCTTC 1 cut(s) 313
SaqAI TTAA 1 cut(s) 861
SatI GCNGC 2 cut(s) 84, 825
Sau3AI GATC 4 cut(s) 112, 247, 357, 728
Sau96I GGNCC 2 cut(s) 596, 628
ScrFI CCNGG 3 cut(s) 173, 383, 1046
SduI GDGCHC 1 cut(s) 308
SfaNI GCATC 4 cut(s) 28, 145, 259, 1008
SfuI TTCGAA 2 cut(s) 425, 896
SinI GGWCC 2 cut(s) 596, 628
SmlI CTYRAG 2 cut(s) 283, 467
SmoI CTYRAG 2 cut(s) 283, 467
Sse9I AATT 3 cut(s) 288, 619, 1037
SsiI CCGC 1 cut(s) 187
SspI AATATT 1 cut(s) 859
SspMI CTAG 4 cut(s) 263, 452, 666, 815
StyD4I CCNGG 3 cut(s) 171, 381, 1044
StyI CCWWGG 1 cut(s) 624
TaaI ACNGT 4 cut(s) 415, 540, 682, 866
TaqI TCGA 2 cut(s) 425, 896
TasI AATT 3 cut(s) 288, 619, 1037
TatI WGTACW 1 cut(s) 682
TfiI GAWTC 5 cut(s) 62, 204, 458, 786, 905
Tru1I TTAA 1 cut(s) 861
Tru9I TTAA 1 cut(s) 861
TscAI CASTG 2 cut(s) 105, 420
TseFI GTSAC 2 cut(s) 182, 668
TseI GCWGC 2 cut(s) 83, 824
Tsp45I GTSAC 2 cut(s) 182, 668
TspDTI ATGAA 6 cut(s) 281, 330, 366, 765, 918, 966
TspRI CASTG 2 cut(s) 105, 420
Van91I CCANNNNNTGG 2 cut(s) 79, 637
VpaK11BI GGWCC 2 cut(s) 596, 628
XapI RAATTY 2 cut(s) 288, 619
XceI RCATGY 1 cut(s) 987
XmiI GTMKAC 2 cut(s) 546, 758
XspI CTAG 4 cut(s) 263, 452, 666, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.