Rroxscaffold_3G00262260

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012442
Physical Location & Seq
Forward (+)
56299240 .. 56301218
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262260.1

Sequence Viewer

Length: 1059 bp
ATGGATTTGGCACATTGCTGCTTAGAGGGAAATGCTGATGCGGTGGAGTTTTGTTCGCACGAATCGTATCGCCATGTTCTGGCGGCTTCAACTTACACATTGCAAGAGGGCGATCAGCCGAGTCGAGCAGGAAGCATATCGCTTTTCAGTGTTGATGCTGACACGGCTCGCCTGGATTTGTGTCAGCGCATTGAAACGGCGGGAATCTTTGATATAAAGTGGAACCCAGTTGGAGGATGCGGTGATCCTCTTCTTGCTCAAGCTGATGCTGATGGTTACTTGAGAATTCTCACTCTTGGGTGCTCTTCAGATGAAGCAAGAGGGTTTTCTTTGAGAGAGATAGCTGATGAAAAGATCAGTTCTTCCATGTGCCTTTTCCTGGATTGGAACCCTTCAGCCACATCTATCACCGTGGGGCTTTCGGATGGTTCTGTCTCCATAGTTTCAGTGGGGGAGTCGCAGCTTGAGACTCAAGAATCGTGGAAAGCGCATGATTTTGAGGTTTGGACAACTTCATTTGATACCCACCAGCCCCAGTTAGTATACACCGGCTCAGATGACTGCAAATTCAAATGCTGGGATTTGCGAGATGGTCCATCCAAGTTGGCATTTCAGAATTCCAAGGTCCATACAATGGGAGTTACTTGCATAGCCAAAAACCCCAACGACCCCAATACTGTACTTACTGGTAGCTATGATGAGTACCTAAGGGCATGGGATGTAAGATCAATATCAAAACCAGTTAATGAAACGTCTACTTGTTTAGGAGGAGGAGTCTGGAGAATCAAGTACCATCCGTTTGTAAGCGGTCTAGTCTTGGCAGCTTGTATGCACAATGGCTTTTCTATTGTCAAGATTAATGGGGATAAAACTGAAGTCATTGAAACATACACCAAGCATGAGTCACTTGCATATGGAGCTGATTGGCATAGAGAGGTATCATTCCATGAAGGCAAAGAAAATAGCACTTTAGTCGCAACGTGCTCGTTTTATGACCGCCTTCTTCGGATATGGATGCCCAAAAGTGATTTGAAGTTGCACCAGGACATTGTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

352

Amino Acids

38.95

Weight (kDa)

5.01

Isoelectric Point (pI)

31.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 113 - 251 1.1e-10 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 126 - 199 6.6e-08 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 126 - 265 1.2e-11 WDR3 first beta-propeller domain
WDR55 PF24796 127 - 250 1.3e-08 WDR55
WDR55 PF24796 127 - 265 7.3e-09 WDR55
WD40_Prp19 PF24814 127 - 280 5.1e-07 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 127 - 239 8.3e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 128 - 243 1.4e-10 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 135 - 252 6.9e-06 TEP-1 second beta-propeller
Beta-prop_EIPR1 PF23609 141 - 276 4.2e-08 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 206 - 316 5.6e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012442)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63010
fragaria_vesca FvH4_5g05540 FvH4_5g05540
malus_domestica MD06G1171600.v1.1
prunus_persica Prupe.5G171000_v2.0.a1 Prupe.5G171000_v2.0.a1
pyrus_communis pycom06g15270
rosa_chinensis RchiOBHm_Chr7g0193241
rosa_laevigata RLG00000004284
rosa_multiflora Rmu_ssc0000092.1_g000021
rosa_roxburghii Rroxscaffold_3G00262260
rosa_rugosa Rorug07G0008400 Rorug07G0008400 Rorug07G0008400
rosa_samantha Rh7AG134000 Rh7BG135000 Rh7CG138000 Rh7DG136000
rosa_wichuraiana Rw7G011460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 79, 634
AccI GTMKAC 2 cut(s) 543, 755
AciI CCGC 6 cut(s) 41, 83, 200, 240, 807, 997
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 3 cut(s) 285, 566, 616
AcuI CTGAAG 3 cut(s) 291, 378, 894
AfaI GTAC 3 cut(s) 681, 704, 791
AfiI CCNNNNNNNGG 4 cut(s) 79, 233, 379, 634
AgsI TTSAA 5 cut(s) 90, 194, 571, 884, 1033
AjnI CCWGG 3 cut(s) 171, 378, 1041
AluBI AGCT 6 cut(s) 263, 344, 463, 693, 824, 920
AluI AGCT 6 cut(s) 263, 344, 463, 693, 824, 920
Alw21I GWGCWC 2 cut(s) 305, 986
Alw26I GTCTC 2 cut(s) 439, 461
AlwI GGATC 1 cut(s) 239
ApeKI GCWGC 3 cut(s) 18, 460, 821
ApoI RAATTY 3 cut(s) 285, 566, 616
AseI ATTAAT 1 cut(s) 858
AspLEI GCGC 2 cut(s) 189, 490
AspS9I GGNCC 2 cut(s) 593, 625
AsuHPI GGTGA 2 cut(s) 254, 400
AvaII GGWCC 2 cut(s) 593, 625
AxyI CCTNAGG 1 cut(s) 707
Bbv12I GWGCWC 2 cut(s) 305, 986
BbvI GCAGC 3 cut(s) 5, 472, 833
BccI CCATC 5 cut(s) 266, 419, 584, 604, 801
BceAI ACGGC 2 cut(s) 180, 213
BcgI CGANNNNNNTGC 4 cut(s) 955, 966, 989, 1000
BciT130I CCWGG 3 cut(s) 173, 380, 1043
BcoDI GTCTC 2 cut(s) 439, 461
BfaI CTAG 1 cut(s) 812
BisI GCNGC 4 cut(s) 19, 84, 461, 822
BlsI GCNGC 4 cut(s) 20, 85, 462, 823
Bme1390I CCNGG 3 cut(s) 173, 380, 1043
Bme18I GGWCC 2 cut(s) 593, 625
BmgT120I GGNCC 2 cut(s) 593, 625
BmiI GGNNCC 2 cut(s) 224, 389
BmrFI CCNGG 3 cut(s) 173, 380, 1043
BmrI ACTGGG 2 cut(s) 221, 529
BmsI GCATC 5 cut(s) 28, 145, 227, 256, 1005
BmuI ACTGGG 2 cut(s) 221, 529
BpmI CTGGAG 1 cut(s) 799
BpuEI CTTGAG 4 cut(s) 243, 301, 456, 485
BsaBI GATNNNNATC 1 cut(s) 730
BsaJI CCNNGG 2 cut(s) 411, 621
BsaXI ACNNNNNCTCC 2 cut(s) 759, 789
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 233, 379, 634
Bse118I RCCGGY 1 cut(s) 548
Bse1I ACTGG 4 cut(s) 227, 535, 691, 740
Bse21I CCTNAGG 1 cut(s) 707
Bse3DI GCAATG 2 cut(s) 13, 98
Bse8I GATNNNNATC 1 cut(s) 730
BseBI CCWGG 3 cut(s) 173, 380, 1043
BseDI CCNNGG 2 cut(s) 411, 621
BseGI GGATG 6 cut(s) 242, 430, 596, 724, 793, 1020
BseJI GATNNNNATC 1 cut(s) 730
BseLI CCNNNNNNNGG 4 cut(s) 79, 233, 379, 634
BseMI GCAATG 2 cut(s) 13, 98
BseMII CTCAG 1 cut(s) 567
BseNI ACTGG 4 cut(s) 227, 535, 691, 740
BseRI GAGGAG 2 cut(s) 783, 786
BseXI GCAGC 3 cut(s) 5, 472, 833
BseYI CCCAGC 1 cut(s) 576
BsiHKAI GWGCWC 2 cut(s) 305, 986
BsiSI CCGG 1 cut(s) 549
BslI CCNNNNNNNGG 4 cut(s) 79, 233, 379, 634
BsmAI GTCTC 2 cut(s) 439, 461
Bsp1286I GDGCHC 2 cut(s) 305, 986
Bsp143I GATC 4 cut(s) 112, 244, 354, 725
BspACI CCGC 6 cut(s) 41, 83, 200, 240, 807, 997
BspCNI CTCAG 1 cut(s) 566
BspLI GGNNCC 2 cut(s) 224, 389
BspPI GGATC 1 cut(s) 239
BspQI GCTCTTC 1 cut(s) 310
BsrDI GCAATG 2 cut(s) 13, 98
BsrFI RCCGGY 1 cut(s) 548
BsrI ACTGG 4 cut(s) 227, 535, 691, 740
BssAI RCCGGY 1 cut(s) 548
BssECI CCNNGG 2 cut(s) 411, 621
BssMI GATC 4 cut(s) 112, 244, 354, 725
BssNAI GTATAC 1 cut(s) 544
BssT1I CCWWGG 1 cut(s) 621
Bst1107I GTATAC 1 cut(s) 544
Bst2UI CCWGG 3 cut(s) 173, 380, 1043
Bst4CI ACNGT 2 cut(s) 412, 679
Bst6I CTCTTC 2 cut(s) 255, 310
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 3 cut(s) 22, 553, 707
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 6 cut(s) 242, 430, 596, 724, 793, 1020
BstHHI GCGC 2 cut(s) 189, 490
BstKTI GATC 4 cut(s) 115, 247, 357, 728
BstMAI GTCTC 2 cut(s) 439, 461
BstMBI GATC 4 cut(s) 112, 244, 354, 725
BstMWI GCNNNNNNNGC 2 cut(s) 164, 917
BstNI CCWGG 3 cut(s) 173, 380, 1043
BstSCI CCNGG 3 cut(s) 171, 378, 1041
BstV1I GCAGC 3 cut(s) 5, 472, 833
BstZ17I GTATAC 1 cut(s) 544
Bsu36I CCTNAGG 1 cut(s) 707
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 6 cut(s) 242, 430, 596, 724, 793, 1020
BtsIMutI CAGTG 2 cut(s) 154, 453
Cac8I GCNNGC 1 cut(s) 169
CfoI GCGC 2 cut(s) 189, 490
Cfr10I RCCGGY 1 cut(s) 548
Cfr13I GGNCC 2 cut(s) 593, 625
Csp6I GTAC 3 cut(s) 680, 703, 790
CspCI CAANNNNNGTGG 2 cut(s) 461, 496
CviAII CATG 6 cut(s) 74, 367, 491, 714, 899, 947
CviQI GTAC 3 cut(s) 680, 703, 790
DdeI CTNAG 3 cut(s) 22, 553, 707
DpnI GATC 4 cut(s) 114, 246, 356, 727
DpnII GATC 4 cut(s) 112, 244, 354, 725
Eam1104I CTCTTC 2 cut(s) 255, 310
EarI CTCTTC 2 cut(s) 255, 310
Eco130I CCWWGG 1 cut(s) 621
Eco47I GGWCC 2 cut(s) 593, 625
Eco57I CTGAAG 3 cut(s) 291, 378, 894
Eco81I CCTNAGG 1 cut(s) 707
EcoRI GAATTC 2 cut(s) 285, 616
EcoRII CCWGG 3 cut(s) 171, 378, 1041
EcoT14I CCWWGG 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 6 cut(s) 77, 370, 494, 717, 902, 950
FatI CATG 6 cut(s) 73, 366, 490, 713, 898, 946
FauI CCCGC 1 cut(s) 193
FauNDI CATATG 1 cut(s) 913
FblI GTMKAC 2 cut(s) 543, 755
Fnu4HI GCNGC 4 cut(s) 19, 84, 461, 822
FokI GGATG 6 cut(s) 249, 437, 583, 731, 780, 1027
Fsp4HI GCNGC 4 cut(s) 19, 84, 461, 822
FspBI CTAG 1 cut(s) 812
GlaI GCGC 2 cut(s) 188, 489
GluI GCNGC 4 cut(s) 19, 84, 461, 822
GsaI CCCAGC 1 cut(s) 580
GsuI CTGGAG 1 cut(s) 799
HapII CCGG 1 cut(s) 549
HhaI GCGC 2 cut(s) 189, 490
Hin1II CATG 6 cut(s) 77, 370, 494, 717, 902, 950
Hin6I GCGC 2 cut(s) 187, 488
HinP1I GCGC 2 cut(s) 187, 488
HinfI GANTC 9 cut(s) 62, 121, 204, 455, 469, 476, 774, 783, 902
HpaII CCGG 1 cut(s) 549
HphI GGTGA 2 cut(s) 254, 400
Hpy166II GTNNAC 2 cut(s) 544, 756
Hpy188I TCNGA 5 cut(s) 310, 424, 556, 615, 1008
Hpy188III TCNNGA 3 cut(s) 473, 778, 853
Hpy8I GTNNAC 2 cut(s) 544, 756
HpyAV CCTTC 3 cut(s) 402, 944, 1010
HpyCH4III ACNGT 2 cut(s) 412, 679
HpyCH4IV ACGT 2 cut(s) 752, 980
HpyCH4V TGCA 6 cut(s) 103, 564, 648, 832, 911, 1039
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 917
HpyF3I CTNAG 3 cut(s) 22, 553, 707
HpySE526I ACGT 2 cut(s) 752, 980
Hsp92II CATG 6 cut(s) 77, 370, 494, 717, 902, 950
HspAI GCGC 2 cut(s) 187, 488
Kzo9I GATC 4 cut(s) 112, 244, 354, 725
LguI GCTCTTC 1 cut(s) 310
LmnI GCTCC 1 cut(s) 917
Lsp1109I GCAGC 3 cut(s) 5, 472, 833
LweI GCATC 5 cut(s) 28, 145, 227, 256, 1005
MaeI CTAG 1 cut(s) 812
MaeII ACGT 2 cut(s) 752, 980
MaeIII GTNAC 3 cut(s) 275, 640, 903
MalI GATC 4 cut(s) 114, 246, 356, 727
MboI GATC 4 cut(s) 112, 244, 354, 725
MboII GAAGA 4 cut(s) 242, 297, 354, 995
MhlI GDGCHC 2 cut(s) 305, 986
MluCI AATT 3 cut(s) 285, 566, 616
MlyI GAGTC 5 cut(s) 130, 463, 464, 783, 911
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 9 cut(s) 19, 100, 227, 258, 314, 493, 761, 764, 928
MseI TTAA 2 cut(s) 744, 858
MspI CCGG 1 cut(s) 549
MspR9I CCNGG 3 cut(s) 173, 380, 1043
MvaI CCWGG 3 cut(s) 173, 380, 1043
MwoI GCNNNNNNNGC 2 cut(s) 164, 917
NdeI CATATG 1 cut(s) 913
NdeII GATC 4 cut(s) 112, 244, 354, 725
NlaIII CATG 6 cut(s) 77, 370, 494, 717, 902, 950
NlaIV GGNNCC 2 cut(s) 224, 389
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 903
PciSI GCTCTTC 1 cut(s) 310
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PfeI GAWTC 4 cut(s) 62, 204, 476, 783
PflMI CCANNNNNTGG 2 cut(s) 79, 634
PfoI TCCNGGA 1 cut(s) 378
PkrI GCNGC 4 cut(s) 20, 85, 462, 823
PleI GAGTC 5 cut(s) 129, 463, 463, 782, 910
PpsI GAGTC 5 cut(s) 129, 463, 463, 782, 910
PshBI ATTAAT 1 cut(s) 858
Psp6I CCWGG 3 cut(s) 171, 378, 1041
PspFI CCCAGC 1 cut(s) 576
PspGI CCWGG 3 cut(s) 171, 378, 1041
PspN4I GGNNCC 2 cut(s) 224, 389
PspPI GGNCC 2 cut(s) 593, 625
RsaI GTAC 3 cut(s) 681, 704, 791
RsaNI GTAC 3 cut(s) 680, 703, 790
SapI GCTCTTC 1 cut(s) 310
SaqAI TTAA 2 cut(s) 744, 858
SatI GCNGC 4 cut(s) 19, 84, 461, 822
Sau3AI GATC 4 cut(s) 112, 244, 354, 725
Sau96I GGNCC 2 cut(s) 593, 625
SchI GAGTC 5 cut(s) 130, 463, 464, 783, 911
ScrFI CCNGG 3 cut(s) 173, 380, 1043
SduI GDGCHC 2 cut(s) 305, 986
SfaNI GCATC 5 cut(s) 28, 145, 227, 256, 1005
SinI GGWCC 2 cut(s) 593, 625
SmlI CTYRAG 4 cut(s) 258, 280, 464, 471
SmoI CTYRAG 4 cut(s) 258, 280, 464, 471
Sse9I AATT 3 cut(s) 285, 566, 616
SsiI CCGC 6 cut(s) 41, 83, 200, 240, 807, 997
SspMI CTAG 1 cut(s) 812
StyD4I CCNGG 3 cut(s) 171, 378, 1041
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 2 cut(s) 412, 679
TaiI ACGT 2 cut(s) 755, 983
TaqI TCGA 1 cut(s) 124
TasI AATT 3 cut(s) 285, 566, 616
TatI WGTACW 1 cut(s) 679
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 4 cut(s) 62, 204, 476, 783
Tru1I TTAA 2 cut(s) 744, 858
Tru9I TTAA 2 cut(s) 744, 858
TscAI CASTG 2 cut(s) 154, 453
TseFI GTSAC 1 cut(s) 903
TseI GCWGC 3 cut(s) 18, 460, 821
Tsp45I GTSAC 1 cut(s) 903
TspDTI ATGAA 5 cut(s) 327, 363, 504, 762, 963
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 2 cut(s) 154, 453
Van91I CCANNNNNTGG 2 cut(s) 79, 634
VpaK11BI GGWCC 2 cut(s) 593, 625
VspI ATTAAT 1 cut(s) 858
XapI RAATTY 3 cut(s) 285, 566, 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XmiI GTMKAC 2 cut(s) 543, 755
XspI CTAG 1 cut(s) 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.