Rw7G011460

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012442
Physical Location & Seq
Reverse (-)
10760421 .. 10762302
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G011460.1

Sequence Viewer

Length: 1071 bp
ATGGATTTGGCACATTGCTGCTTGGAGGGAAATGCTGATGCGGTGGAGTTTTGTCCGCACGAATCGTATCGCCATGTTCTGGCGGCTTCAACTTACACATTGCAAGAGGGCGATCTGCCGAGTCGAGCAGGAAGCATATCGCTTTTCAGTGTTGATGCTGACACGGCTCGCCTGGATTTGTGTCAGCGCATTGCAACGGTGGGAATCTTTGATATAAAGTGGAACCCAGTTGGAGAGTGCGGTGATCCTCTTCTCGCTCAAGCTGATGCTGATGGCTACTTGAGAATTCTCAGTCTTGGGTCCTCTTCAGATGAAGCAAAAGGGTTTTCTTTGAGAGAGATAGCTGACGAAAAGGTCAGTTCTTCCATGTGCCTTTTCCTGGATTGGAACCCTTCAGCCACATCCATCACCGTGGGGCTTTCGGATGGTTCTGTCTCGATAGTTTCAGTGGGGGAGTCGCAGCTTGAGACACAAGAATCGTGGAAAGCGCATGATTTTGAGGTTTGGACAACCTCATTTGATACCCACCAGCCCCAGTTAGTATACAGCGGCTCAGATGACTGCAAATTCAAATGCTGGGATTTGCGAGATGGTCCATCGAAGTTGGCATTTCAGAATTCCAAGGTCCATACAATGGGAGTTACTTGCATAGCCAAAAACCCCAACGACCCCAATACTGTACTTACTGGTAGCTATGATGAGTACCTAAGGGTATGGGATGTAAGATCAATATCAAAACCAGTTAATGAAACGTCTACTTGTTTAGGAGGAGGAGTCTGGAGAATCAAGTACCATCCGTTTGTAAGCGGTCTAGTCTTGGCAGCTTGTATGCACAATGGCTTTTCTATTGTCAAGATTTATGGGGATAAAACTGAAGTCATTGAAACATACACTAAGCATGAGTCACTTGCATATGGAGCTGATTGGCATAGAGAGGTATCATTCTATGAAGGCAAAGAAAATAGCACTTTAGTCGCAACGTGCTCGTTTTATGACCGCCTTCTCCGGATATGGATGCCCAAAAGTGATTTGAAGTTGCACGAGGACATTGTTTTGATCACATCTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

356

Amino Acids

39.39

Weight (kDa)

4.91

Isoelectric Point (pI)

32.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 69 - 279 8.9e-13 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 110 - 250 8.7e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 125 - 265 7.6e-13 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 126 - 199 8.8e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 127 - 301 7.9e-12 WDR5 beta-propeller domain
WDR55 PF24796 127 - 252 3.7e-09 WDR55
WD40_Prp19 PF24814 127 - 290 3.8e-08 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 127 - 239 8.5e-07 WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd PF25047 135 - 252 7.5e-07 TEP-1 second beta-propeller
Beta-prop_EIPR1 PF23609 140 - 276 2.4e-08 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 206 - 301 3.5e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012442)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63010
fragaria_vesca FvH4_5g05540 FvH4_5g05540
malus_domestica MD06G1171600.v1.1
prunus_persica Prupe.5G171000_v2.0.a1 Prupe.5G171000_v2.0.a1
pyrus_communis pycom06g15270
rosa_chinensis RchiOBHm_Chr7g0193241
rosa_laevigata RLG00000004284
rosa_multiflora Rmu_ssc0000092.1_g000021
rosa_roxburghii Rroxscaffold_3G00262260
rosa_rugosa Rorug07G0008400 Rorug07G0008400 Rorug07G0008400
rosa_samantha Rh7AG134000 Rh7BG135000 Rh7CG138000 Rh7DG136000
rosa_wichuraiana Rw7G011460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
AccB7I CCANNNNNTGG 2 cut(s) 79, 634
AccI GTMKAC 2 cut(s) 543, 755
AccIII TCCGGA 1 cut(s) 1005
AciI CCGC 7 cut(s) 41, 56, 83, 240, 549, 807, 997
AclWI GGATC 1 cut(s) 239
AcsI RAATTY 3 cut(s) 285, 566, 616
AcuI CTGAAG 3 cut(s) 291, 378, 894
AfaI GTAC 3 cut(s) 681, 704, 791
AfiI CCNNNNNNNGG 3 cut(s) 79, 379, 634
AgsI TTSAA 4 cut(s) 90, 571, 884, 1033
AjnI CCWGG 2 cut(s) 171, 378
AluBI AGCT 6 cut(s) 263, 344, 463, 693, 824, 920
AluI AGCT 6 cut(s) 263, 344, 463, 693, 824, 920
Alw21I GWGCWC 1 cut(s) 986
Alw26I GTCTC 2 cut(s) 439, 461
AlwI GGATC 1 cut(s) 239
Aor13HI TCCGGA 1 cut(s) 1005
ApeKI GCWGC 3 cut(s) 18, 460, 821
ApoI RAATTY 3 cut(s) 285, 566, 616
AspLEI GCGC 2 cut(s) 189, 490
AspS9I GGNCC 3 cut(s) 300, 593, 625
AsuHPI GGTGA 2 cut(s) 254, 400
AvaII GGWCC 3 cut(s) 300, 593, 625
AxyI CCTNAGG 1 cut(s) 707
BauI CACGAG 1 cut(s) 1040
Bbv12I GWGCWC 1 cut(s) 986
BbvI GCAGC 3 cut(s) 5, 472, 833
BccI CCATC 6 cut(s) 266, 413, 419, 584, 604, 801
BceAI ACGGC 1 cut(s) 180
BcgI CGANNNNNNTGC 4 cut(s) 955, 966, 989, 1000
BciT130I CCWGG 2 cut(s) 173, 380
BclI TGATCA 1 cut(s) 1056
BcoDI GTCTC 2 cut(s) 439, 461
BfaI CTAG 2 cut(s) 812, 1069
BisI GCNGC 5 cut(s) 19, 84, 461, 550, 822
BlsI GCNGC 5 cut(s) 20, 85, 462, 551, 823
Bme1390I CCNGG 2 cut(s) 173, 380
Bme18I GGWCC 3 cut(s) 300, 593, 625
BmgT120I GGNCC 3 cut(s) 300, 593, 625
BmiI GGNNCC 3 cut(s) 224, 301, 389
BmrFI CCNGG 2 cut(s) 173, 380
BmrI ACTGGG 2 cut(s) 221, 529
BmsI GCATC 4 cut(s) 28, 145, 256, 1005
BmuI ACTGGG 2 cut(s) 221, 529
BpmI CTGGAG 1 cut(s) 799
BpuEI CTTGAG 3 cut(s) 243, 301, 485
BsaBI GATNNNNATC 1 cut(s) 730
BsaJI CCNNGG 2 cut(s) 411, 621
BsaWI WCCGGW 1 cut(s) 1005
BsaXI ACNNNNNCTCC 2 cut(s) 759, 789
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 379, 634
Bse1I ACTGG 4 cut(s) 227, 535, 691, 740
Bse21I CCTNAGG 1 cut(s) 707
Bse3DI GCAATG 3 cut(s) 13, 98, 189
Bse8I GATNNNNATC 1 cut(s) 730
BseAI TCCGGA 1 cut(s) 1005
BseBI CCWGG 2 cut(s) 173, 380
BseDI CCNNGG 2 cut(s) 411, 621
BseGI GGATG 5 cut(s) 401, 430, 724, 793, 1020
BseJI GATNNNNATC 1 cut(s) 730
BseLI CCNNNNNNNGG 3 cut(s) 79, 379, 634
BseMI GCAATG 3 cut(s) 13, 98, 189
BseMII CTCAG 2 cut(s) 304, 567
BseNI ACTGG 4 cut(s) 227, 535, 691, 740
BseRI GAGGAG 2 cut(s) 783, 786
BseXI GCAGC 3 cut(s) 5, 472, 833
BseYI CCCAGC 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 986
BsiSI CCGG 1 cut(s) 1006
BslI CCNNNNNNNGG 3 cut(s) 79, 379, 634
BsmAI GTCTC 2 cut(s) 439, 461
Bsp1286I GDGCHC 1 cut(s) 986
Bsp13I TCCGGA 1 cut(s) 1005
Bsp143I GATC 4 cut(s) 112, 244, 725, 1056
BspACI CCGC 7 cut(s) 41, 56, 83, 240, 549, 807, 997
BspCNI CTCAG 2 cut(s) 303, 566
BspEI TCCGGA 1 cut(s) 1005
BspLI GGNNCC 3 cut(s) 224, 301, 389
BspPI GGATC 1 cut(s) 239
BsrDI GCAATG 3 cut(s) 13, 98, 189
BsrI ACTGG 4 cut(s) 227, 535, 691, 740
BssECI CCNNGG 2 cut(s) 411, 621
BssMI GATC 4 cut(s) 112, 244, 725, 1056
BssNAI GTATAC 1 cut(s) 544
BssSI CACGAG 1 cut(s) 1040
BssT1I CCWWGG 1 cut(s) 621
Bst1107I GTATAC 1 cut(s) 544
Bst2BI CACGAG 1 cut(s) 1040
Bst2UI CCWGG 2 cut(s) 173, 380
Bst4CI ACNGT 3 cut(s) 199, 412, 679
Bst6I CTCTTC 2 cut(s) 255, 310
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 4 cut(s) 290, 553, 707, 894
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 5 cut(s) 401, 430, 724, 793, 1020
BstHHI GCGC 2 cut(s) 189, 490
BstKTI GATC 4 cut(s) 115, 247, 728, 1059
BstMAI GTCTC 2 cut(s) 439, 461
BstMBI GATC 4 cut(s) 112, 244, 725, 1056
BstMWI GCNNNNNNNGC 2 cut(s) 164, 917
BstNI CCWGG 2 cut(s) 173, 380
BstSCI CCNGG 2 cut(s) 171, 378
BstV1I GCAGC 3 cut(s) 5, 472, 833
BstXI CCANNNNNNTGG 1 cut(s) 412
BstZ17I GTATAC 1 cut(s) 544
Bsu36I CCTNAGG 1 cut(s) 707
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 5 cut(s) 401, 430, 724, 793, 1020
BtsIMutI CAGTG 2 cut(s) 154, 453
Cac8I GCNNGC 1 cut(s) 169
CfoI GCGC 2 cut(s) 189, 490
Cfr13I GGNCC 3 cut(s) 300, 593, 625
Csp6I GTAC 3 cut(s) 680, 703, 790
CspCI CAANNNNNGTGG 2 cut(s) 461, 496
CviAII CATG 4 cut(s) 74, 367, 491, 899
CviQI GTAC 3 cut(s) 680, 703, 790
DdeI CTNAG 4 cut(s) 290, 553, 707, 894
DpnI GATC 4 cut(s) 114, 246, 727, 1058
DpnII GATC 4 cut(s) 112, 244, 725, 1056
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
Eam1104I CTCTTC 2 cut(s) 255, 310
EarI CTCTTC 2 cut(s) 255, 310
Eco130I CCWWGG 1 cut(s) 621
Eco47I GGWCC 3 cut(s) 300, 593, 625
Eco57I CTGAAG 3 cut(s) 291, 378, 894
Eco81I CCTNAGG 1 cut(s) 707
EcoO109I RGGNCCY 1 cut(s) 300
EcoRI GAATTC 2 cut(s) 285, 616
EcoRII CCWGG 2 cut(s) 171, 378
EcoT14I CCWWGG 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 621
FaeI CATG 4 cut(s) 77, 370, 494, 902
FatI CATG 4 cut(s) 73, 366, 490, 898
FauNDI CATATG 1 cut(s) 913
FbaI TGATCA 1 cut(s) 1056
FblI GTMKAC 2 cut(s) 543, 755
Fnu4HI GCNGC 5 cut(s) 19, 84, 461, 550, 822
FokI GGATG 5 cut(s) 388, 437, 731, 780, 1027
Fsp4HI GCNGC 5 cut(s) 19, 84, 461, 550, 822
FspBI CTAG 2 cut(s) 812, 1069
GlaI GCGC 2 cut(s) 188, 489
GluI GCNGC 5 cut(s) 19, 84, 461, 550, 822
GsaI CCCAGC 1 cut(s) 580
GsuI CTGGAG 1 cut(s) 799
HapII CCGG 1 cut(s) 1006
HhaI GCGC 2 cut(s) 189, 490
Hin1II CATG 4 cut(s) 77, 370, 494, 902
Hin6I GCGC 2 cut(s) 187, 488
HinP1I GCGC 2 cut(s) 187, 488
HinfI GANTC 8 cut(s) 62, 121, 204, 455, 476, 774, 783, 902
HpaII CCGG 1 cut(s) 1006
HphI GGTGA 2 cut(s) 254, 400
Hpy166II GTNNAC 2 cut(s) 544, 756
Hpy188I TCNGA 4 cut(s) 310, 424, 556, 615
Hpy188III TCNNGA 4 cut(s) 436, 778, 853, 1006
Hpy8I GTNNAC 2 cut(s) 544, 756
HpyAV CCTTC 3 cut(s) 402, 944, 1010
HpyCH4III ACNGT 3 cut(s) 199, 412, 679
HpyCH4IV ACGT 2 cut(s) 752, 980
HpyCH4V TGCA 7 cut(s) 103, 194, 564, 648, 832, 911, 1039
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 917
HpyF3I CTNAG 4 cut(s) 290, 553, 707, 894
HpySE526I ACGT 2 cut(s) 752, 980
Hsp92II CATG 4 cut(s) 77, 370, 494, 902
HspAI GCGC 2 cut(s) 187, 488
Kpn2I TCCGGA 1 cut(s) 1005
Ksp22I TGATCA 1 cut(s) 1056
Kzo9I GATC 4 cut(s) 112, 244, 725, 1056
LmnI GCTCC 1 cut(s) 917
Lsp1109I GCAGC 3 cut(s) 5, 472, 833
LweI GCATC 4 cut(s) 28, 145, 256, 1005
MaeI CTAG 2 cut(s) 812, 1069
MaeII ACGT 2 cut(s) 752, 980
MaeIII GTNAC 2 cut(s) 640, 903
MalI GATC 4 cut(s) 114, 246, 727, 1058
MboI GATC 4 cut(s) 112, 244, 725, 1056
MboII GAAGA 4 cut(s) 242, 297, 354, 1056
MhlI GDGCHC 1 cut(s) 986
MluCI AATT 3 cut(s) 285, 566, 616
MlyI GAGTC 4 cut(s) 130, 464, 783, 911
MmeI TCCRAC 1 cut(s) 211
MroI TCCGGA 1 cut(s) 1005
MseI TTAA 1 cut(s) 744
MslI CAYNNNNRTG 1 cut(s) 410
MspA1I CMGCKG 1 cut(s) 549
MspI CCGG 1 cut(s) 1006
MspR9I CCNGG 2 cut(s) 173, 380
MvaI CCWGG 2 cut(s) 173, 380
MwoI GCNNNNNNNGC 2 cut(s) 164, 917
NdeI CATATG 1 cut(s) 913
NdeII GATC 4 cut(s) 112, 244, 725, 1056
NlaIII CATG 4 cut(s) 77, 370, 494, 902
NlaIV GGNNCC 3 cut(s) 224, 301, 389
NmeAIII GCCGAG 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 903
PfeI GAWTC 4 cut(s) 62, 204, 476, 783
PflMI CCANNNNNTGG 2 cut(s) 79, 634
PfoI TCCNGGA 1 cut(s) 378
PkrI GCNGC 5 cut(s) 20, 85, 462, 551, 823
PleI GAGTC 4 cut(s) 129, 463, 782, 910
PpsI GAGTC 4 cut(s) 129, 463, 782, 910
PpuMI RGGWCCY 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 300
Psp6I CCWGG 2 cut(s) 171, 378
PspFI CCCAGC 1 cut(s) 576
PspGI CCWGG 2 cut(s) 171, 378
PspN4I GGNNCC 3 cut(s) 224, 301, 389
PspPI GGNCC 3 cut(s) 300, 593, 625
PspPPI RGGWCCY 1 cut(s) 300
RsaI GTAC 3 cut(s) 681, 704, 791
RsaNI GTAC 3 cut(s) 680, 703, 790
RseI CAYNNNNRTG 1 cut(s) 410
SaqAI TTAA 1 cut(s) 744
SatI GCNGC 5 cut(s) 19, 84, 461, 550, 822
Sau3AI GATC 4 cut(s) 112, 244, 725, 1056
Sau96I GGNCC 3 cut(s) 300, 593, 625
SchI GAGTC 4 cut(s) 130, 464, 783, 911
ScrFI CCNGG 2 cut(s) 173, 380
SduI GDGCHC 1 cut(s) 986
SfaNI GCATC 4 cut(s) 28, 145, 256, 1005
SinI GGWCC 3 cut(s) 300, 593, 625
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 3 cut(s) 258, 280, 464
SmoI CTYRAG 3 cut(s) 258, 280, 464
Sse9I AATT 3 cut(s) 285, 566, 616
SsiI CCGC 7 cut(s) 41, 56, 83, 240, 549, 807, 997
SspMI CTAG 2 cut(s) 812, 1069
StyD4I CCNGG 2 cut(s) 171, 378
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 3 cut(s) 199, 412, 679
TaiI ACGT 2 cut(s) 755, 983
TaqI TCGA 3 cut(s) 124, 437, 599
TasI AATT 3 cut(s) 285, 566, 616
TatI WGTACW 1 cut(s) 679
TauI GCSGC 2 cut(s) 86, 552
TfiI GAWTC 4 cut(s) 62, 204, 476, 783
Tru1I TTAA 1 cut(s) 744
Tru9I TTAA 1 cut(s) 744
TscAI CASTG 2 cut(s) 154, 453
TseFI GTSAC 1 cut(s) 903
TseI GCWGC 3 cut(s) 18, 460, 821
Tsp45I GTSAC 1 cut(s) 903
TspDTI ATGAA 3 cut(s) 327, 762, 963
TspGWI ACGGA 1 cut(s) 786
TspRI CASTG 2 cut(s) 154, 453
Van91I CCANNNNNTGG 2 cut(s) 79, 634
VpaK11BI GGWCC 3 cut(s) 300, 593, 625
XapI RAATTY 3 cut(s) 285, 566, 616
XmiI GTMKAC 2 cut(s) 543, 755
XspI CTAG 2 cut(s) 812, 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.