MD06G1171600.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012442
Physical Location & Seq
Reverse (-)
31197099 .. 31198862
1764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1171600.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGCGAATGGATGTGGCACATTGTAATTTGGAGGGCAATGCCGATGCCGTAGAGTTTTGCCCGCACGATTCATACCGCCATGTTCTGGCAGCTTCAACGTACACGCTGCAAGAGGGTGAACAACCGATCCGAGCTGGAAGTATATCGCTGTTCAACGTTGGTGCTGACTTGGGTCGGCTTGATTTGTTTCAGCGGATTGAAACGGCAGGGATTTTCGATATAAAGTGGAACCCAGTTGGAGACAGCGTGAGTCCTCTTCTTGCTCAAGCCGACGCTGATGGTTACTTGAGAATTCACGGTCTTGAATCCTGCTCAGATGAAGCACAAGGGTTATCTTTGAGGGAGATAACTGATGAAAATATCAGTTCATCCATGTGCCTTTTCCTGGATTGGAACCCTTCAGCCACTTCTATCACCGTGGGGCTTTCGAATGGTTCTGTCGCTATAGCTTCAATTGGGGAATCCCAACTTGAGACACAAGAAACATGGAAAGCGCATGATTTTGAGGTTTGGACAGCTTCGTATGATATCCACCAGCCCCAGTTGGTGTATAGTGGTTCAGATGACTGCAAATTCAAATGCTGGGATTTGCGAGACGGTCCTTCGAAGTTGGCATTTCAGAACACCAAAGCCCATACAATGGGAGTTTGCTGCATAGCCAAAAATCCCTACGATTCGAATTGTGTAGTTACTGGCAGCTATGATGAGTACCTGAGGTTTTGGGATGTAAGATCAATATCAACACCAGTTAATGAAACCTCTGTTTGCTTAGGGGGAGGAGTGTGGAGAATCAAGTTCCATCCTTTTGTGGCCGGTCTCGTCTTGACAGCTTGTATGCACAACGGATTTTCGATAGTCAAGATTAATGAGGATAAAACTGAAGTGATTGAAACGTACAGCAAGCACGAGTCACTTGCATATGGAGCTGACTGGCACAGAGACAAATCGTTCCATGAAGGCAAAAGGAATAGCACTTTAGTTGCTACTTGCTCATTTTATGACCGTCTTCTTCGTTTATGGATGCCGGAAAGTGATTTGCAGGATTCTGTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

352

Amino Acids

39.06

Weight (kDa)

4.9

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 114 - 277 1.2e-12 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 128 - 315 6.1e-14 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 128 - 269 3.4e-13 WDR3 first beta-propeller domain
WDR55 PF24796 129 - 268 5.8e-09 WDR55
Beta-prop_WDR5 PF25175 132 - 245 2.4e-11 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 137 - 262 2.9e-06 TEP-1 second beta-propeller
WD40_Prp19 PF24814 141 - 268 1.9e-06 Prp19 WD40 domain
Beta-prop_EIPR1 PF23609 143 - 278 1.5e-08 EIPR1 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012442)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63010
fragaria_vesca FvH4_5g05540 FvH4_5g05540
malus_domestica MD06G1171600.v1.1
prunus_persica Prupe.5G171000_v2.0.a1 Prupe.5G171000_v2.0.a1
pyrus_communis pycom06g15270
rosa_chinensis RchiOBHm_Chr7g0193241
rosa_laevigata RLG00000004284
rosa_multiflora Rmu_ssc0000092.1_g000021
rosa_roxburghii Rroxscaffold_3G00262260
rosa_rugosa Rorug07G0008400 Rorug07G0008400 Rorug07G0008400
rosa_samantha Rh7AG134000 Rh7BG135000 Rh7CG138000 Rh7DG136000
rosa_wichuraiana Rw7G011460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 85, 640
AciI CCGC 3 cut(s) 62, 76, 193
AclI AACGTT 1 cut(s) 156
AclWI GGATC 1 cut(s) 121
AcoI YGGCCR 1 cut(s) 810
AcsI RAATTY 2 cut(s) 291, 572
AcuI CTGAAG 2 cut(s) 384, 900
AfaI GTAC 3 cut(s) 101, 710, 896
AfiI CCNNNNNNNGG 3 cut(s) 85, 385, 640
AgsI TTSAA 7 cut(s) 96, 154, 200, 305, 453, 577, 890
AjnI CCWGG 1 cut(s) 384
AloI GAACNNNNNNTCC 2 cut(s) 111, 143
AluBI AGCT 7 cut(s) 92, 134, 449, 518, 699, 830, 926
AluI AGCT 7 cut(s) 92, 134, 449, 518, 699, 830, 926
Alw26I GTCTC 5 cut(s) 234, 467, 588, 821, 933
AlwI GGATC 1 cut(s) 121
AoxI GGCC 1 cut(s) 810
ApeKI GCWGC 4 cut(s) 89, 106, 651, 696
ApoI RAATTY 2 cut(s) 291, 572
AseI ATTAAT 1 cut(s) 864
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 1 cut(s) 599
AsuHPI GGTGA 2 cut(s) 128, 406
AsuII TTCGAA 3 cut(s) 428, 605, 677
AvaII GGWCC 1 cut(s) 599
AxyI CCTNAGG 1 cut(s) 713
BauI CACGAG 1 cut(s) 905
BbsI GAAGAC 1 cut(s) 998
BbvI GCAGC 4 cut(s) 93, 101, 638, 708
BccI CCATC 2 cut(s) 272, 807
BceAI ACGGC 2 cut(s) 32, 219
BcgI CGANNNNNNTGC 2 cut(s) 896, 930
BciT130I CCWGG 1 cut(s) 386
BcoDI GTCTC 5 cut(s) 234, 467, 588, 821, 933
BfmI CTRYAG 1 cut(s) 444
BisI GCNGC 4 cut(s) 90, 107, 652, 697
BlsI GCNGC 4 cut(s) 91, 108, 653, 698
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 599
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 2 cut(s) 230, 395
BmrFI CCNGG 1 cut(s) 386
BmrI ACTGGG 2 cut(s) 227, 535
BmsI GCATC 2 cut(s) 34, 1011
BmuI ACTGGG 2 cut(s) 227, 535
BoxI GACNNNNGTC 1 cut(s) 171
BpiI GAAGAC 1 cut(s) 998
Bpu10I CCTNAGC 1 cut(s) 769
Bpu14I TTCGAA 3 cut(s) 428, 605, 677
BpuEI CTTGAG 3 cut(s) 249, 307, 491
BsaBI GATNNNNATC 1 cut(s) 736
BsaI GGTCTC 1 cut(s) 821
BsaJI CCNNGG 1 cut(s) 417
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 385, 640
Bse118I RCCGGY 1 cut(s) 812
Bse1I ACTGG 5 cut(s) 233, 541, 697, 746, 935
Bse21I CCTNAGG 1 cut(s) 713
Bse3DI GCAATG 1 cut(s) 43
Bse8I GATNNNNATC 1 cut(s) 736
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 5 cut(s) 16, 368, 730, 799, 1026
BseJI GATNNNNATC 1 cut(s) 736
BseLI CCNNNNNNNGG 3 cut(s) 85, 385, 640
BseMI GCAATG 1 cut(s) 43
BseMII CTCAG 2 cut(s) 327, 704
BseNI ACTGG 5 cut(s) 233, 541, 697, 746, 935
BseRI GAGGAG 1 cut(s) 792
BseXI GCAGC 4 cut(s) 93, 101, 638, 708
BseYI CCCAGC 1 cut(s) 582
BshFI GGCC 1 cut(s) 812
BsiSI CCGG 2 cut(s) 813, 1025
BslI CCNNNNNNNGG 3 cut(s) 85, 385, 640
BsmAI GTCTC 5 cut(s) 234, 467, 588, 821, 933
BsmBI CGTCTC 1 cut(s) 588
BsnI GGCC 1 cut(s) 812
Bso31I GGTCTC 1 cut(s) 821
Bsp119I TTCGAA 3 cut(s) 428, 605, 677
Bsp143I GATC 2 cut(s) 126, 731
BspACI CCGC 3 cut(s) 62, 76, 193
BspANI GGCC 1 cut(s) 812
BspCNI CTCAG 2 cut(s) 326, 705
BspLI GGNNCC 2 cut(s) 230, 395
BspPI GGATC 1 cut(s) 121
BspT104I TTCGAA 3 cut(s) 428, 605, 677
BspTNI GGTCTC 1 cut(s) 821
BsrDI GCAATG 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 812
BsrI ACTGG 5 cut(s) 233, 541, 697, 746, 935
BssAI RCCGGY 1 cut(s) 812
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 2 cut(s) 126, 731
BssSI CACGAG 1 cut(s) 905
Bst2BI CACGAG 1 cut(s) 905
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 4 cut(s) 299, 418, 599, 1004
Bst6I CTCTTC 1 cut(s) 261
BstBI TTCGAA 3 cut(s) 428, 605, 677
BstC8I GCNNGC 2 cut(s) 62, 902
BstDEI CTNAG 3 cut(s) 313, 713, 769
BstDSI CCRYGG 1 cut(s) 417
BstF5I GGATG 5 cut(s) 16, 368, 730, 799, 1026
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 2 cut(s) 129, 734
BstMAI GTCTC 5 cut(s) 234, 467, 588, 821, 933
BstMBI GATC 2 cut(s) 126, 731
BstMWI GCNNNNNNNGC 1 cut(s) 923
BstNI CCWGG 1 cut(s) 386
BstPAI GACNNNNGTC 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 384
BstSFI CTRYAG 1 cut(s) 444
BstV1I GCAGC 4 cut(s) 93, 101, 638, 708
BstV2I GAAGAC 1 cut(s) 998
Bsu36I CCTNAGG 1 cut(s) 713
BsuRI GGCC 1 cut(s) 812
BtgI CCRYGG 1 cut(s) 417
BtsCI GGATG 5 cut(s) 16, 368, 730, 799, 1026
Cac8I GCNNGC 2 cut(s) 62, 902
CfoI GCGC 1 cut(s) 496
Cfr10I RCCGGY 1 cut(s) 812
Cfr13I GGNCC 1 cut(s) 599
CseI GACGC 1 cut(s) 281
Csp6I GTAC 3 cut(s) 100, 709, 895
CviAII CATG 5 cut(s) 80, 373, 486, 497, 953
CviQI GTAC 3 cut(s) 100, 709, 895
DdeI CTNAG 3 cut(s) 313, 713, 769
DpnI GATC 2 cut(s) 128, 733
DpnII GATC 2 cut(s) 126, 731
EaeI YGGCCR 1 cut(s) 810
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco31I GGTCTC 1 cut(s) 821
Eco32I GATATC 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 2 cut(s) 384, 900
Eco81I CCTNAGG 1 cut(s) 713
EcoRI GAATTC 1 cut(s) 291
EcoRII CCWGG 1 cut(s) 384
EcoRV GATATC 1 cut(s) 529
Esp3I CGTCTC 1 cut(s) 588
FaeI CATG 5 cut(s) 83, 376, 489, 500, 956
FatI CATG 5 cut(s) 79, 372, 485, 496, 952
FauI CCCGC 1 cut(s) 69
FauNDI CATATG 1 cut(s) 919
Fnu4HI GCNGC 4 cut(s) 90, 107, 652, 697
FokI GGATG 5 cut(s) 23, 355, 737, 786, 1033
Fsp4HI GCNGC 4 cut(s) 90, 107, 652, 697
GlaI GCGC 1 cut(s) 495
GluI GCNGC 4 cut(s) 90, 107, 652, 697
GsaI CCCAGC 1 cut(s) 586
HaeIII GGCC 1 cut(s) 812
HapII CCGG 2 cut(s) 813, 1025
HgaI GACGC 1 cut(s) 281
HhaI GCGC 1 cut(s) 496
Hin1II CATG 5 cut(s) 83, 376, 489, 500, 956
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HinfI GANTC 8 cut(s) 68, 250, 305, 461, 674, 789, 908, 1043
HpaII CCGG 2 cut(s) 813, 1025
HphI GGTGA 2 cut(s) 128, 406
Hpy166II GTNNAC 2 cut(s) 102, 119
Hpy188I TCNGA 4 cut(s) 131, 316, 562, 621
Hpy188III TCNNGA 3 cut(s) 302, 823, 859
Hpy8I GTNNAC 2 cut(s) 102, 119
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 3 cut(s) 408, 612, 950
HpyCH4III ACNGT 4 cut(s) 299, 418, 599, 1004
HpyCH4IV ACGT 3 cut(s) 98, 156, 893
HpyCH4V TGCA 6 cut(s) 109, 570, 654, 838, 917, 1039
HpyF10VI GCNNNNNNNGC 1 cut(s) 923
HpyF3I CTNAG 3 cut(s) 313, 713, 769
HpySE526I ACGT 3 cut(s) 98, 156, 893
Hsp92II CATG 5 cut(s) 83, 376, 489, 500, 956
HspAI GCGC 1 cut(s) 494
Kzo9I GATC 2 cut(s) 126, 731
LmnI GCTCC 1 cut(s) 923
Lsp1109I GCAGC 4 cut(s) 93, 101, 638, 708
LweI GCATC 2 cut(s) 34, 1011
MaeII ACGT 3 cut(s) 98, 156, 893
MaeIII GTNAC 3 cut(s) 281, 688, 909
MalI GATC 2 cut(s) 128, 733
MboI GATC 2 cut(s) 126, 731
MboII GAAGA 3 cut(s) 248, 998, 1001
MfeI CAATTG 1 cut(s) 453
MluCI AATT 5 cut(s) 25, 291, 453, 572, 679
MlyI GAGTC 2 cut(s) 259, 917
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 9 cut(s) 25, 106, 264, 333, 499, 708, 769, 770, 862
MseI TTAA 2 cut(s) 750, 864
MslI CAYNNNNRTG 1 cut(s) 373
MspA1I CMGCKG 1 cut(s) 193
MspI CCGG 2 cut(s) 813, 1025
MspR9I CCNGG 1 cut(s) 386
MunI CAATTG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 386
MwoI GCNNNNNNNGC 1 cut(s) 923
NdeI CATATG 1 cut(s) 919
NdeII GATC 2 cut(s) 126, 731
NlaIII CATG 5 cut(s) 83, 376, 489, 500, 956
NlaIV GGNNCC 2 cut(s) 230, 395
NmuCI GTSAC 1 cut(s) 909
NspV TTCGAA 3 cut(s) 428, 605, 677
PfeI GAWTC 6 cut(s) 68, 305, 461, 674, 789, 1043
PflMI CCANNNNNTGG 2 cut(s) 85, 640
PfoI TCCNGGA 1 cut(s) 384
PkrI GCNGC 4 cut(s) 91, 108, 653, 698
PleI GAGTC 2 cut(s) 258, 916
PpsI GAGTC 2 cut(s) 258, 916
PshAI GACNNNNGTC 1 cut(s) 171
PshBI ATTAAT 1 cut(s) 864
Psp1406I AACGTT 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 384
PspFI CCCAGC 1 cut(s) 582
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 2 cut(s) 230, 395
PspPI GGNCC 1 cut(s) 599
RsaI GTAC 3 cut(s) 101, 710, 896
RsaNI GTAC 3 cut(s) 100, 709, 895
RseI CAYNNNNRTG 1 cut(s) 373
SaqAI TTAA 2 cut(s) 750, 864
SatI GCNGC 4 cut(s) 90, 107, 652, 697
Sau3AI GATC 2 cut(s) 126, 731
Sau96I GGNCC 1 cut(s) 599
SchI GAGTC 2 cut(s) 259, 917
ScrFI CCNGG 1 cut(s) 386
SfaNI GCATC 2 cut(s) 34, 1011
SfcI CTRYAG 1 cut(s) 444
SfuI TTCGAA 3 cut(s) 428, 605, 677
SinI GGWCC 1 cut(s) 599
SmiMI CAYNNNNRTG 1 cut(s) 373
SmlI CTYRAG 3 cut(s) 264, 286, 470
SmoI CTYRAG 3 cut(s) 264, 286, 470
Sse9I AATT 5 cut(s) 25, 291, 453, 572, 679
SsiI CCGC 3 cut(s) 62, 76, 193
StyD4I CCNGG 1 cut(s) 384
TaaI ACNGT 4 cut(s) 299, 418, 599, 1004
TaiI ACGT 3 cut(s) 101, 159, 896
TaqI TCGA 5 cut(s) 216, 428, 605, 677, 851
TasI AATT 5 cut(s) 25, 291, 453, 572, 679
TfiI GAWTC 6 cut(s) 68, 305, 461, 674, 789, 1043
Tru1I TTAA 2 cut(s) 750, 864
Tru9I TTAA 2 cut(s) 750, 864
TseFI GTSAC 1 cut(s) 909
TseI GCWGC 4 cut(s) 89, 106, 651, 696
Tsp45I GTSAC 1 cut(s) 909
TspDTI ATGAA 6 cut(s) 60, 333, 357, 369, 768, 969
TspGWI ACGGA 1 cut(s) 858
Van91I CCANNNNNTGG 2 cut(s) 85, 640
VpaK11BI GGWCC 1 cut(s) 599
VspI ATTAAT 1 cut(s) 864
XapI RAATTY 2 cut(s) 291, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.