FvH4_5g05780

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013666
Physical Location & Seq
Reverse (-)
3419125 .. 3424142
5018 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05780.t1

Sequence Viewer

Length: 3339 bp
ATGCCTGTAAATCCATGGACCTTCTTCTTCTCCTTCTTAACCCTCTCTTCTTTTCTTCTGGTTCACTTCCCTGCTCTGGCCTTGAACCAACAAGGCCAGGCTCTTGTTTCATGGAAGCAGAGCCTCAATGGCTCCCCTGAGGGGCTGAGCAATTGGGACCCGAGTGATGAAACTCCATGTGGGTGGTTTGGGGTCACCTGCAACTTCAACAACCAAGTTGTGGAGCTGAATTTGAAGTATATTGATCTTCTGGGTAAGGTGCCTTCGAATTTCACTTCATTACTGACGCTAAACAAGCTAGTCTTGTCAGGCACAAACCTCACCGGCTCAATCCCAAGAGAAATCTCCACTCTTAAGCAACTGACCTCACTCGACCTCAGCGACAATGCACTGAGCGGCGAAATCCCAGTCGAAATCTGCGAGCTGCCTAAACTACAAGAGCTCTACCTCAGCACGAACCGGCTCGAAGGCTCAATCCCAGTCCAAATCGGAAACCTCACAAGCCTCACGTGGTTTGTTGTGTACGACAATCAGCTCAGCGGCAACATTCCTAGCACCATCGGAAATCTACCCCAATTGCAAGTAATCAGAGCCGGAGGGAACAAGAATCTTGAAGGTGCTCTACCCGATGAGATTGGTAACTGCACCAATCTGGTCATGTTGGGACTAGCAGAAACCAGCATTTCCGGGTTCCTACCTCCAAGCCTCGGCATTCTTAAAAAGCTCGAAACTTTGGCAGTCTACACCACTCTCGTCTCCGGTCCGATCCCCCCGGAGCTAGGAGACTGCACCGAGCTCCGGGACGTATACTTGTACGAAAACTCGCTCAGTGGCTCTGTTCCGAGCAAATTGGGCAACTTGAAGAATCTCCAGAACCTTCTGCTGTGGCAGAACAGCCTTGTGGGGGTTATTCCACCGGAGCTAGGCAACTGTCACCAGCTATTAGTCATTGACATCTCTATGAATTCCCTCACCGGAAGCATTCCTCAGTCTTTTGGGAACTTGACGTCGCTTCAGGAGCTTCAGTTGAGTGTGAATCAAATCTCCGGCGAGATCCCGGCGAAGCTAGGAAACTGCAGGCAACTCACTCATATCGAGATGGATAATAATCAGATCACCGGGACTATTCCTTTCGAATTCGGAAGCTTATCGAATCTCACCATACTATTCTTGTGGCAAAACAAGCTTGAAGGTGCTATTCCTGCTTCAATTTCCAACTGTGGCAATCTTGAAGCGGTTGATTTGTCGCAGAACGGGTTGACCGGTCCGATTCCCGGCGGGATTTTCCAGCTCCAGAAACTCACCAAGCTGCTACTCCTGTCGAACAATATCTCCGGCGAGATTCCGCCGGAGATCGGAAACTGCTCGTCGTTGATTCGGTTCCGGGCGAACGACAACAAGCTAACCGGAGCAATCCCGCAGCAGATTGGGAGCTTGAAGAGCTTGAATTTCCTTGATCTCGGATCGAATCGGCTCAACGGGAACATACCGGAGGGGATCTCCGGCTGCCGGAATCTGACGTTTCTCGACTTGCATTCGAATTCGATCACCGGAAACTTGCCTGCGGGTTTCAATCTGCTTGTGTCTCTCCAGTTCGTCGATTTCTCGGATAATATGATCGAAGGGGTATTGAGTCCTGGTCTCGGTTCGCTGACGTCACTCACAAAGTTTAGCTTGGGGAAGAACCGGTTCACCGGTTCGATTCCGAGTCAGATCGGTTCGTGTGGGAAGTTGCAATTACTGGACCTTGGCGGGAACGAGCTGACCGGTGTGATTCCGGCGAGTTTGGGAAAGATTCCGGCGCTGGAAATCTCTCTGAACCTGAGCTGGAACCAACTCTCCGGCGAGTTACCGAAGGAGTTCGCCGATTTGGACAAGCTCGGGATTCTCGACCTCTCCCACAACCAGCTCTCCGGCGACCTCCAATTTCTCGCGGACATGCAAAATCTCGTCGTCCTCAACGTCTCGCACAACAACTTCACCGGGCGCGTCCCGGACACGCCCTTCTTCGCGAAGCTCCCGCTCAGCGTCATGTCCGGCAACCCCGCCCTCTGCCTCACCGGCAGCCAGTGCGCCGCCGCGAACCCGACCTGGTCCCGCCGCCGCAACGCCGCCGCGCGCGTGGCGATGGTCGTCCTTCTCTGCACCGCATGCACGCTCCTCTTAGCCGCGCTGTACATCATCCTCGCCTCCCGAAAGCGCGCGCAGCCAGGGTTCTTCGGCGGTGCCCACGAGCCCGACCCGGAAGACGACTCCGAAGAAGTCGACGTGGGCCCACCTTGGGAGGTGACGCTCTACCAGAAACTCGACCTCTCTATCGTCGACGTGGCGAAATCCCTGACTCCCGCCAACGTCATAGGCCGGGGTCGATCCGGCGTCGTCTACCACGTGTCCATCCCCTCGGGACTCTCGCTCGCCGTGAAAAGATTCCGCACCGGCGAGAAACACTCCGCTTCCGCGTTTTCCTCCGAGATTGCCACGCTGGCGAGAATACGTCACCGTAACATCGTGAGGTTACTTGGGTGGGGGGCCAACAGGAGAACGAAGTTACTGTTTTACGATTACCTACCCCAAGGTAACTTGGGGTCGTTGCTCCACGAGGGGTGCGCAGGCTTAGTAGAGTGGGACACGCGGTTCAAGATAGCACTGGGCGTGGCGGAGGGGTTGGCGTATTTGCACCACGATTGCCAGCCGGCTATTTTGCACCGGGACGTGAAGGCCCAGAATATTTTACTTGGGGATCAATACGAGGCCGTTTTGGCCGACTTTGGGTTGGCTAGGTTGGTTGAGGACGATCAAAACGGACCGTTTTCAGCTAACCCTCAGTTTGCTGGTTCTTACGGCTACATTGCGCCTGAATATGCCTGTATGCTGAAAATTACTGCAAAAAGCGATGTCTATAGCTATGGAGTAGTCCTTTTGGAGATCATAACTGGGAAAAGGCCGGTAGACCCATCGTTCACTGACGGCCAGCATGTGATTCAGTGGGTGCGAGATCACTTGAAGAGCAAGAAGGACCCGGTAGAGATTCTTGATCAGAAGCTTCAAGGCTACCCGGATACGCAGATACAAGAAATGCTTCAAGCACTTGGAATTTCGCTCCTTTGTACTAGCAACAGGGCGGAAGATCGTCCCACGATGAAGGATGTGGCTGCATTGTTGAGAGAAATTCGACATGATCAGCCTGCGACAGGAAGCGAGGCGCACAAGCCTGCTAGCACCGCGTTGAAGAACTCATCGTCTTCTGTGACTCCAGCTCAACTGCTGATGATGCAAGGGTCTTCTCATTGCTCACTTGCCTACTCTTCTTCAGCTGGTTATCTATCAGGAACACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1113

Amino Acids

120.09

Weight (kDa)

5.5

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 68 1e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 90 - 197 3.8e-11 Leucine-rich repeat region
LRR_14 PF23598 260 - 369 1.3e-08 Leucine-rich repeat region
LRR_8 PF13855 289 - 348 8.9e-08 Leucine rich repeat
LRR_8 PF13855 314 - 372 2.6e-06 Leucine rich repeat
LRR_8 PF13855 360 - 420 2.6e-06 Leucine rich repeat
LRR_8 PF13855 384 - 444 4.5e-06 Leucine rich repeat
LRR_8 PF13855 464 - 516 1e-05 Leucine rich repeat
LRR_8 PF13855 553 - 613 2.8e-06 Leucine rich repeat
LRR_8 PF13855 577 - 637 2.8e-06 Leucine rich repeat
Pkinase PF00069 784 - 1050 5.2e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 784 - 1052 1.4e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013666)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05780 FvH4_5g05780 FvH4_5g05780
malus_domestica MD06G1170200.v1.1
prunus_persica Prupe.5G169700_v2.0.a1
pyrus_communis pycom06g15160
rosa_chinensis RchiOBHm_Chr7g0193041
rosa_laevigata RLG00000004301
rosa_multiflora Rmu_co8431803.1_g000001 Rmu_co8478551.1_g000001 Rmu_sc0002040.1_g000078
rosa_roxburghii Rroxscaffold_3G00262440
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132300 Rh7BG133200 Rh7CG136300 Rh7DG134200
rosa_wichuraiana Rw7G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 206
AatII GACGTC 2 cut(s) 1010, 1658
Acc16I TGCGCA 1 cut(s) 2608
Acc36I ACCTGC 1 cut(s) 206
AccB1I GGYRCC 2 cut(s) 259, 2225
AccB7I CCANNNNNTGG 1 cut(s) 220
AccBSI CCGCTC 2 cut(s) 396, 2023
AccI GTMKAC 6 cut(s) 741, 807, 2265, 2322, 2382, 2949
AclWI GGATC 6 cut(s) 760, 1048, 1471, 1505, 2364, 2748
AcoI YGGCCR 2 cut(s) 2760, 2968
AcsI RAATTY 8 cut(s) 229, 268, 964, 1136, 1447, 1540, 3093, 3168
AcuI CTGAAG 3 cut(s) 998, 1007, 3294
AcvI CACGTG 2 cut(s) 510, 2389
AcyI GRCGYC 3 cut(s) 1007, 1655, 2376
AfaI GTAC 4 cut(s) 524, 815, 2177, 3109
AflII CTTAAG 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 2388
AgeI ACCGGT 4 cut(s) 1262, 1686, 1694, 1766
AjiI CACGTC 3 cut(s) 2269, 2326, 2713
AjnI CCWGG 4 cut(s) 96, 1636, 2090, 2209
AloI GAACNNNNNNTCC 6 cut(s) 1316, 1348, 1823, 1855, 2618, 2650
Alw21I GWGCWC 3 cut(s) 444, 622, 798
Alw26I GTCTC 5 cut(s) 760, 777, 1590, 1646, 1969
AlwI GGATC 6 cut(s) 760, 1048, 1471, 1505, 2364, 2748
Ama87I CYCGRG 3 cut(s) 160, 1880, 2401
ApaI GGGCCC 1 cut(s) 2276
ApeKI GCWGC 7 cut(s) 424, 1309, 1420, 1506, 2064, 2206, 3152
ApoI RAATTY 8 cut(s) 229, 268, 964, 1136, 1447, 1540, 3093, 3168
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
AsiGI ACCGGT 4 cut(s) 1262, 1686, 1694, 1766
Asp700I GAANNNNTTC 4 cut(s) 1688, 1859, 2426, 3078
AsuII TTCGAA 3 cut(s) 266, 1134, 1538
AsuNHI GCTAGC 1 cut(s) 3215
AvaI CYCGRG 3 cut(s) 160, 1880, 2401
AvaII GGWCC 8 cut(s) 18, 157, 761, 1265, 1744, 2094, 2804, 3016
AxyI CCTNAGG 1 cut(s) 138
BaeGI GKGCMC 2 cut(s) 2230, 2276
BanI GGYRCC 2 cut(s) 259, 2225
BanII GRGCYC 4 cut(s) 444, 798, 2238, 2276
BarI GAAGNNNNNNTAC 4 cut(s) 247, 279, 606, 638
BauI CACGAG 2 cut(s) 2231, 2597
BbrPI CACGTG 2 cut(s) 510, 2389
BbsI GAAGAC 3 cut(s) 2253, 3234, 3273
Bbv12I GWGCWC 3 cut(s) 444, 622, 798
BbvCI CCTCAGC 2 cut(s) 377, 449
BbvI GCAGC 7 cut(s) 411, 1296, 1432, 1493, 2076, 2218, 3139
BccI CCATC 5 cut(s) 566, 1093, 2122, 2402, 2962
BceAI ACGGC 4 cut(s) 2402, 2738, 2857, 2983
BciT130I CCWGG 4 cut(s) 98, 1638, 2092, 2211
BciVI GTATCC 1 cut(s) 3052
BclI TGATCA 2 cut(s) 3034, 3178
BcoDI GTCTC 5 cut(s) 760, 777, 1590, 1646, 1969
BfaI CTAG 9 cut(s) 299, 552, 668, 779, 923, 1067, 2778, 3111, 3216
BfmI CTRYAG 2 cut(s) 1075, 2899
BfoI RGCGCY 1 cut(s) 1805
BfrI CTTAAG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 206
BfuI GTATCC 1 cut(s) 3052
BglI GCCNNNNNGGC 4 cut(s) 129, 2061, 2483, 2759
BlpI GCTNAGC 3 cut(s) 146, 536, 2024
Bme18I GGWCC 8 cut(s) 18, 157, 761, 1265, 1744, 2094, 2804, 3016
BmeT110I CYCGRG 3 cut(s) 160, 1880, 2401
BmgBI CACGTC 3 cut(s) 2269, 2326, 2713
BmrI ACTGGG 4 cut(s) 401, 473, 2657, 2943
BmsI GCATC 1 cut(s) 3261
BmtI GCTAGC 1 cut(s) 3219
BmuI ACTGGG 4 cut(s) 401, 473, 2657, 2943
BpiI GAAGAC 3 cut(s) 2253, 3234, 3273
BplI GAGNNNNNCTC 4 cut(s) 1484, 1516, 2432, 2464
BpmI CTGGAG 4 cut(s) 854, 1277, 1574, 3237
Bpu10I CCTNAGC 3 cut(s) 377, 449, 1823
Bpu1102I GCTNAGC 3 cut(s) 146, 536, 2024
Bpu14I TTCGAA 3 cut(s) 266, 1134, 1538
BsaAI YACGTR 2 cut(s) 510, 2389
BsaBI GATNNNNATC 1 cut(s) 1107
BsaHI GRCGYC 3 cut(s) 1007, 1655, 2376
BsaI GGTCTC 1 cut(s) 1646
BsaJI CCNNGG 9 cut(s) 14, 706, 771, 1747, 2210, 2279, 2362, 2400, 2572
BsaXI ACNNNNNCTCC 8 cut(s) 1010, 1040, 1316, 1346, 1823, 1853, 1895, 1925
Bse1I ACTGG 7 cut(s) 407, 479, 1591, 1746, 2068, 2652, 2938
Bse21I CCTNAGG 1 cut(s) 138
Bse3DI GCAATG 2 cut(s) 2847, 3286
Bse8I GATNNNNATC 1 cut(s) 1107
BseBI CCWGG 4 cut(s) 98, 1638, 2092, 2211
BseDI CCNNGG 9 cut(s) 14, 706, 771, 1747, 2210, 2279, 2362, 2400, 2572
BseGI GGATG 3 cut(s) 2181, 2394, 3151
BseJI GATNNNNATC 1 cut(s) 1107
BseMI GCAATG 2 cut(s) 2847, 3286
BseNI ACTGG 7 cut(s) 407, 479, 1591, 1746, 2068, 2652, 2938
BsePI GCGCGC 3 cut(s) 2117, 2200, 2202
BseRI GAGGAG 1 cut(s) 2150
BseSI GKGCMC 2 cut(s) 2230, 2276
BseXI GCAGC 7 cut(s) 411, 1296, 1432, 1493, 2076, 2218, 3139
BsgI GTGCAG 3 cut(s) 628, 772, 2128
BshNI GGYRCC 2 cut(s) 259, 2225
BshTI ACCGGT 4 cut(s) 1262, 1686, 1694, 1766
BsiHKAI GWGCWC 3 cut(s) 444, 622, 798
BsiHKCI CYCGRG 3 cut(s) 160, 1880, 2401
BsmAI GTCTC 5 cut(s) 760, 777, 1590, 1646, 1969
BsmBI CGTCTC 2 cut(s) 760, 1969
BsmI GAATGC 3 cut(s) 711, 981, 1534
Bso31I GGTCTC 1 cut(s) 1646
BsoBI CYCGRG 3 cut(s) 160, 1880, 2401
Bsp119I TTCGAA 3 cut(s) 266, 1134, 1538
Bsp120I GGGCCC 1 cut(s) 2272
Bsp1286I GDGCHC 6 cut(s) 444, 622, 798, 2230, 2238, 2276
Bsp1407I TGTACA 1 cut(s) 2175
Bsp1720I GCTNAGC 3 cut(s) 146, 536, 2024
Bsp19I CCATGG 1 cut(s) 14
Bsp68I TCGCGA 1 cut(s) 2012
BspMAI CTGCAG 1 cut(s) 1079
BspMI ACCTGC 1 cut(s) 206
BspOI GCTAGC 1 cut(s) 3219
BspPI GGATC 6 cut(s) 760, 1048, 1471, 1505, 2364, 2748
BspQI GCTCTTC 2 cut(s) 1433, 2999
BspT104I TTCGAA 3 cut(s) 266, 1134, 1538
BspT107I GGYRCC 2 cut(s) 259, 2225
BspTI CTTAAG 1 cut(s) 353
BspTNI GGTCTC 1 cut(s) 1646
BsrBI CCGCTC 2 cut(s) 396, 2023
BsrDI GCAATG 2 cut(s) 2847, 3286
BsrGI TGTACA 1 cut(s) 2175
BsrI ACTGG 7 cut(s) 407, 479, 1591, 1746, 2068, 2652, 2938
BssECI CCNNGG 9 cut(s) 14, 706, 771, 1747, 2210, 2279, 2362, 2400, 2572
BssHII GCGCGC 3 cut(s) 2117, 2200, 2202
BssNAI GTATAC 1 cut(s) 808
BssNI GRCGYC 3 cut(s) 1007, 1655, 2376
BssSI CACGAG 2 cut(s) 2231, 2597
BssT1I CCWWGG 4 cut(s) 14, 1747, 2279, 2572
Bst1107I GTATAC 1 cut(s) 808
Bst2BI CACGAG 2 cut(s) 2231, 2597
Bst2UI CCWGG 4 cut(s) 98, 1638, 2092, 2211
Bst4CI ACNGT 5 cut(s) 932, 1220, 2501, 2553, 2808
Bst6I CTCTTC 4 cut(s) 52, 1433, 2999, 3310
BstACI GRCGYC 3 cut(s) 1007, 1655, 2376
BstAFI CTTAAG 1 cut(s) 353
BstAPI GCANNNNNTGC 2 cut(s) 2070, 2151
BstAUI TGTACA 1 cut(s) 2175
BstBAI YACGTR 2 cut(s) 510, 2389
BstBI TTCGAA 3 cut(s) 266, 1134, 1538
BstDSI CCRYGG 1 cut(s) 14
BstEII GGTNACC 1 cut(s) 193
BstENI CCTNNNNNAGG 1 cut(s) 3189
BstF5I GGATG 3 cut(s) 2181, 2394, 3151
BstH2I RGCGCY 1 cut(s) 1805
BstMAI GTCTC 5 cut(s) 760, 777, 1590, 1646, 1969
BstNI CCWGG 4 cut(s) 98, 1638, 2092, 2211
BstNSI RCATGY 3 cut(s) 1942, 2154, 2978
BstPI GGTNACC 1 cut(s) 193
BstSFI CTRYAG 2 cut(s) 1075, 2899
BstSLI GKGCMC 2 cut(s) 2230, 2276
BstV1I GCAGC 7 cut(s) 411, 1296, 1432, 1493, 2076, 2218, 3139
BstV2I GAAGAC 3 cut(s) 2253, 3234, 3273
BstX2I RGATCY 2 cut(s) 1053, 1497
BstXI CCANNNNNNTGG 1 cut(s) 183
BstYI RGATCY 2 cut(s) 1053, 1497
BstZ17I GTATAC 1 cut(s) 808
Bsu36I CCTNAGG 1 cut(s) 138
BsuI GTATCC 1 cut(s) 3052
BtgI CCRYGG 1 cut(s) 14
BtgZI GCGATG 2 cut(s) 2141, 2907
BtrI CACGTC 3 cut(s) 2269, 2326, 2713
BtsCI GGATG 3 cut(s) 2181, 2394, 3151
BtsIMutI CAGTG 6 cut(s) 389, 835, 2075, 2645, 2961, 2990
BtuMI TCGCGA 1 cut(s) 2012
BveI ACCTGC 1 cut(s) 206
CpoI CGGWCCG 3 cut(s) 761, 1265, 2804
CseI GACGC 5 cut(s) 295, 1978, 2017, 2299, 2365
CsiI ACCWGGT 1 cut(s) 2090
Csp6I GTAC 4 cut(s) 523, 814, 2176, 3108
CspAI ACCGGT 4 cut(s) 1262, 1686, 1694, 1766
CspI CGGWCCG 3 cut(s) 761, 1265, 2804
CviAII CATG 9 cut(s) 15, 111, 177, 658, 1939, 2032, 2151, 2975, 3176
CviQI GTAC 4 cut(s) 523, 814, 2176, 3108
EaeI YGGCCR 2 cut(s) 2760, 2968
Eam1104I CTCTTC 4 cut(s) 52, 1433, 2999, 3310
EarI CTCTTC 4 cut(s) 52, 1433, 2999, 3310
EciI GGCGGA 3 cut(s) 1335, 2672, 3137
Ecl136II GAGCTC 2 cut(s) 442, 796
Eco130I CCWWGG 4 cut(s) 14, 1747, 2279, 2572
Eco24I GRGCYC 4 cut(s) 444, 798, 2238, 2276
Eco31I GGTCTC 1 cut(s) 1646
Eco47I GGWCC 8 cut(s) 18, 157, 761, 1265, 1744, 2094, 2804, 3016
Eco53kI GAGCTC 2 cut(s) 442, 796
Eco57I CTGAAG 3 cut(s) 998, 1007, 3294
Eco72I CACGTG 2 cut(s) 510, 2389
Eco81I CCTNAGG 1 cut(s) 138
Eco88I CYCGRG 3 cut(s) 160, 1880, 2401
Eco91I GGTNACC 1 cut(s) 193
EcoICRI GAGCTC 2 cut(s) 442, 796
EcoNI CCTNNNNNAGG 1 cut(s) 3189
EcoO109I RGGNCCY 2 cut(s) 157, 3016
EcoO65I GGTNACC 1 cut(s) 193
EcoRI GAATTC 3 cut(s) 964, 1136, 1540
EcoRII CCWGG 4 cut(s) 96, 1636, 2090, 2209
EcoT14I CCWWGG 4 cut(s) 14, 1747, 2279, 2572
EcoT38I GRGCYC 4 cut(s) 444, 798, 2238, 2276
ErhI CCWWGG 4 cut(s) 14, 1747, 2279, 2572
Esp3I CGTCTC 2 cut(s) 760, 1969
FaeI CATG 9 cut(s) 18, 114, 180, 661, 1942, 2035, 2154, 2978, 3179
FalI AAGNNNNNCTT 6 cut(s) 287, 319, 1658, 1690, 3063, 3095
FatI CATG 9 cut(s) 14, 110, 176, 657, 1938, 2031, 2150, 2974, 3175
FauI CCCGC 8 cut(s) 1271, 1425, 1558, 1745, 2028, 2053, 2105, 2353
FbaI TGATCA 2 cut(s) 3034, 3178
FblI GTMKAC 6 cut(s) 741, 807, 2265, 2322, 2382, 2949
FokI GGATG 3 cut(s) 2168, 2381, 3158
FriOI GRGCYC 4 cut(s) 444, 798, 2238, 2276
FspBI CTAG 9 cut(s) 299, 552, 668, 779, 923, 1067, 2778, 3111, 3216
FspI TGCGCA 1 cut(s) 2608
GsuI CTGGAG 4 cut(s) 854, 1277, 1574, 3237
HaeII RGCGCY 1 cut(s) 1805
HgaI GACGC 5 cut(s) 295, 1978, 2017, 2299, 2365
Hin1I GRCGYC 3 cut(s) 1007, 1655, 2376
Hin1II CATG 9 cut(s) 18, 114, 180, 661, 1942, 2035, 2154, 2978, 3179
HincII GTYRAC 3 cut(s) 1260, 2266, 2323
HindII GTYRAC 3 cut(s) 1260, 2266, 2323
HindIII AAGCTT 3 cut(s) 1144, 1184, 3041
Hpy99I CGWCG 8 cut(s) 1012, 1372, 1601, 1955, 2270, 2324, 2327, 2381
HpyCH4III ACNGT 5 cut(s) 932, 1220, 2501, 2553, 2808
Hsp92I GRCGYC 3 cut(s) 1007, 1655, 2376
Hsp92II CATG 9 cut(s) 18, 114, 180, 661, 1942, 2035, 2154, 2978, 3179
KflI GGGWCCC 1 cut(s) 157
KroI GCCGGC 1 cut(s) 2692
KroNI GCCGGC 1 cut(s) 2694
Ksp22I TGATCA 2 cut(s) 3034, 3178
LguI GCTCTTC 2 cut(s) 1433, 2999
Lsp1109I GCAGC 7 cut(s) 411, 1296, 1432, 1493, 2076, 2218, 3139
LweI GCATC 1 cut(s) 3261
MabI ACCWGGT 1 cut(s) 2090
MaeI CTAG 9 cut(s) 299, 552, 668, 779, 923, 1067, 2778, 3111, 3216
MauBI CGCGCGCG 1 cut(s) 2117
MbiI CCGCTC 2 cut(s) 396, 2023
MfeI CAATTG 2 cut(s) 151, 575
MflI RGATCY 2 cut(s) 1053, 1497
MhlI GDGCHC 6 cut(s) 444, 622, 798, 2230, 2238, 2276
MlyI GAGTC 6 cut(s) 1642, 1717, 2246, 2335, 2400, 3244
MmeI TCCRAC 1 cut(s) 1239
MroNI GCCGGC 1 cut(s) 2692
MroXI GAANNNNTTC 4 cut(s) 1688, 1859, 2426, 3078
MseI TTAA 3 cut(s) 38, 354, 717
MslI CAYNNNNRTG 2 cut(s) 181, 959
MspA1I CMGCKG 2 cut(s) 540, 3314
MspCI CTTAAG 1 cut(s) 353
MunI CAATTG 2 cut(s) 151, 575
Mva1269I GAATGC 3 cut(s) 711, 981, 1534
MvaI CCWGG 4 cut(s) 98, 1638, 2092, 2211
NaeI GCCGGC 1 cut(s) 2694
NcoI CCATGG 1 cut(s) 14
NgoMIV GCCGGC 1 cut(s) 2692
NheI GCTAGC 1 cut(s) 3215
NlaIII CATG 9 cut(s) 18, 114, 180, 661, 1942, 2035, 2154, 2978, 3179
NmeAIII GCCGAG 1 cut(s) 687
NmuCI GTSAC 6 cut(s) 193, 932, 1656, 2287, 2495, 3247
NruI TCGCGA 1 cut(s) 2012
NsbI TGCGCA 1 cut(s) 2608
NspI RCATGY 3 cut(s) 1942, 2154, 2978
NspV TTCGAA 3 cut(s) 266, 1134, 1538
PaeI GCATGC 1 cut(s) 2154
PaqCI CACCTGC 1 cut(s) 206
PauI GCGCGC 3 cut(s) 2117, 2200, 2202
PciSI GCTCTTC 2 cut(s) 1433, 2999
PcsI WCGNNNNNNNCGW 5 cut(s) 378, 417, 1887, 1959, 2385
PctI GAATGC 3 cut(s) 711, 981, 1534
PdiI GCCGGC 1 cut(s) 2694
PdmI GAANNNNTTC 4 cut(s) 1688, 1859, 2426, 3078
PflFI GACNNNGTC 1 cut(s) 2092
PflMI CCANNNNNTGG 1 cut(s) 220
PfoI TCCNGGA 2 cut(s) 798, 1992
PinAI ACCGGT 4 cut(s) 1262, 1686, 1694, 1766
PleI GAGTC 6 cut(s) 1641, 1716, 2246, 2335, 2400, 3244
PmaCI CACGTG 2 cut(s) 510, 2389
PmlI CACGTG 2 cut(s) 510, 2389
PpsI GAGTC 6 cut(s) 1641, 1716, 2246, 2335, 2400, 3244
Ppu21I YACGTR 2 cut(s) 510, 2389
PpuMI RGGWCCY 2 cut(s) 157, 3016
Psp124BI GAGCTC 2 cut(s) 444, 798
Psp5II RGGWCCY 2 cut(s) 157, 3016
Psp6I CCWGG 4 cut(s) 96, 1636, 2090, 2209
PspCI CACGTG 2 cut(s) 510, 2389
PspEI GGTNACC 1 cut(s) 193
PspGI CCWGG 4 cut(s) 96, 1636, 2090, 2209
PspOMI GGGCCC 1 cut(s) 2272
PspPPI RGGWCCY 2 cut(s) 157, 3016
PstI CTGCAG 1 cut(s) 1079
PsuI RGATCY 2 cut(s) 1053, 1497
PsyI GACNNNGTC 1 cut(s) 2092
PteI GCGCGC 3 cut(s) 2117, 2200, 2202
PvuII CAGCTG 1 cut(s) 3314
RruI TCGCGA 1 cut(s) 2012
RsaI GTAC 4 cut(s) 524, 815, 2177, 3109
RsaNI GTAC 4 cut(s) 523, 814, 2176, 3108
RseI CAYNNNNRTG 2 cut(s) 181, 959
Rsr2I CGGWCCG 3 cut(s) 761, 1265, 2804
RsrII CGGWCCG 3 cut(s) 761, 1265, 2804
SacI GAGCTC 2 cut(s) 444, 798
SalI GTCGAC 2 cut(s) 2264, 2321
SapI GCTCTTC 2 cut(s) 1433, 2999
SaqAI TTAA 3 cut(s) 38, 354, 717
SchI GAGTC 6 cut(s) 1642, 1717, 2246, 2335, 2400, 3244
SduI GDGCHC 6 cut(s) 444, 622, 798, 2230, 2238, 2276
SexAI ACCWGGT 1 cut(s) 2090
SfaNI GCATC 1 cut(s) 3261
SfcI CTRYAG 2 cut(s) 1075, 2899
SfiI GGCCNNNNNGGCC 1 cut(s) 2759
SfuI TTCGAA 3 cut(s) 266, 1134, 1538
SgrAI CRCCGGYG 1 cut(s) 2435
SgrDI CGTCGACG 1 cut(s) 2321
SinI GGWCC 8 cut(s) 18, 157, 761, 1265, 1744, 2094, 2804, 3016
SmiMI CAYNNNNRTG 2 cut(s) 181, 959
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SphI GCATGC 1 cut(s) 2154
SspI AATATT 1 cut(s) 2728
SspMI CTAG 9 cut(s) 299, 552, 668, 779, 923, 1067, 2778, 3111, 3216
SstI GAGCTC 2 cut(s) 444, 798
StyI CCWWGG 4 cut(s) 14, 1747, 2279, 2572
TaaI ACNGT 5 cut(s) 932, 1220, 2501, 2553, 2808
TatI WGTACW 2 cut(s) 2175, 3107
TauI GCSGC 9 cut(s) 399, 543, 2078, 2081, 2103, 2106, 2114, 2117, 2171
Tru1I TTAA 3 cut(s) 38, 354, 717
Tru9I TTAA 3 cut(s) 38, 354, 717
TscAI CASTG 6 cut(s) 396, 835, 2075, 2652, 2968, 2990
TseFI GTSAC 6 cut(s) 193, 932, 1656, 2287, 2495, 3247
TseI GCWGC 7 cut(s) 424, 1309, 1420, 1506, 2064, 2206, 3152
Tsp45I GTSAC 6 cut(s) 193, 932, 1656, 2287, 2495, 3247
TspDTI ATGAA 5 cut(s) 99, 183, 267, 977, 3155
TspGWI ACGGA 1 cut(s) 2817
TspRI CASTG 6 cut(s) 396, 835, 2075, 2652, 2968, 2990
Tth111I GACNNNGTC 1 cut(s) 2092
Van91I CCANNNNNTGG 1 cut(s) 220
Vha464I CTTAAG 1 cut(s) 353
VpaK11BI GGWCC 8 cut(s) 18, 157, 761, 1265, 1744, 2094, 2804, 3016
XagI CCTNNNNNAGG 1 cut(s) 3189
XapI RAATTY 8 cut(s) 229, 268, 964, 1136, 1447, 1540, 3093, 3168
XceI RCATGY 3 cut(s) 1942, 2154, 2978
XmiI GTMKAC 6 cut(s) 741, 807, 2265, 2322, 2382, 2949
XmnI GAANNNNTTC 4 cut(s) 1688, 1859, 2426, 3078
XspI CTAG 9 cut(s) 299, 552, 668, 779, 923, 1067, 2778, 3111, 3216
ZraI GACGTC 2 cut(s) 1008, 1656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.