Rw7G011290

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013666
Physical Location & Seq
Forward (+)
10584300 .. 10588678
4379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G011290.1

Sequence Viewer

Length: 3225 bp
ATGCCTGTAAATCCATGGACCTTCCTCTTCTTCTTAACCCTCTCTTCTATTCTTCTGGTTCACTTTCCTGCTCTGGCCTTGAACCAACAAGGCCAGGCTCTTGTTTCATGGAAGCAGAGTCTCAATGGCTCCCCTGAGGGGCTGAGCAATTGGGACCCAAGTGATGAAACTCCATGCGGGTGGTCCGGGGTCACCTGCAACTTCAACAACCAAGTTGCGGAGCTGAATTTGAAGTATATGGATCTTCTAGGTAAGGTGCCCTGGAATTTCACTTCGTTGCTGACCCTAAACAAGCTCATCTTGTCCGGCACAAACCTCACCGGCTCAATCCCACGAGAAATCTCTACTCTTAAACAACTGACCTCACTGGACCTGAGCGACAATGCACTGAGCGGCGAAATCCCAGTTGAGATCTTCGAGCTGCCTAAGCTCGAAGAACTCTACCTCAGCACGAACCGGCTGGAGGGCTCAATCCCAGTCCAAATCGGAAACCTCACAAACCTGAAGCGGCTGGTGCTCTACGACAATCAGCTCAGCGGGAAAATTCCTAGCACCATTGGAAATCTGCCTCAACTTCAAGTAATCAGAGCTGGAGGGAACAAGAACCTTGAAGGTCCTGTGCCTCATGAGATTGGGAACTGCACCGATTTGGTCATGTTGGGCTTAGCAGAAACCAGCATTTCCGGCTTCCTCCCTCCAAGCCTTGGCCTCCTCAAAAAACTCGAGACATTGGCAATCTACACCACCCTCCTCCGGGACATCTACTTGTACGAAAACTCCATTAGCGGCTCTGTTCCGAGCAAATTAGGCAACTCGAAAAATCTTCAAAACCTTCTGCTATGGCAAAACAGCCTTGTAGGGGTTCTTCCACCGGAGCTCGGCAATTGTCACCAGCTATTGGTCATTGACATGTCTATGAATTCCTTGACCGGAAGCATTCCTCAGTCTTTTGGGAACTTGACCTCGCTTCAGGAGCTCCAATTGAGTGTCAATCAAATCTCAGGCGAGATCCCGGCGCAGCTAGGAAACTGCCGGCAACTCACTCATATCGAGCTGGATAACAACCAGATCACCGGAACGATTCCTTTCGAATTCGGCCGCTTATCCAATCTCACGATCCTCTTCTTGTGGCAAAACAAGCTTGAAGGAGCCATTCCTTCATCGATTTCCAACTGTGGCAATCTTGAAGCGGTTGATTTCTCACAAAACTTTTTGACCGGTCCCATTCCGGGTGGGATTTTCCAGCTCCAGAATCTCACCAAGCTTCTACTACTGTCGAACAATCTCTCCAGCGAAATCCCGCCCGAGATCGGAAACTGTTCCTCGTTGATTCGGTTTCGGGCGAACAACAACAAGCTCACCGGAGCAATCCCGCAGCAGATAGGGAGTTTGAAGAGCTTGAATTTCCTCGATCTCGGATCGAATCGGCTCAACCGAAACATACCCGAGGGGATCGCCGGCTGCCGGAATCTGACGTTTCTCGACCTGCATTCCAATTCGATCACCGGAAACTTGCCTGCAGGCTTCAATCTGCTTGTGTCTCTCCAGTTCGTCGATTTCTCGGATAATATGATCGAAGGGACGTTGAGTCCTGGACTCGGTTCGCTGACGTCACTCACCAAGCTCGTTTTGGGGAACAACCGGTTCGCCGGTTCCATTCCGAGTCAACTCGGTTCGTGTGGGAAGTTACAGTTACTGGACCTTGGCGGGAACGAGCTAACAGGTACAATCCCGGCGACTTTGGGAAAGATTCCGGCACTGGAAATCTCTCTGAACCTGAGCTGGAACCAACTCTCCGGCGAGTTACCGAAGGAGTTCGCCGATTTGGACAAGCTCGGCATTCTGGACCTCTCTCACAACCAGCTTTCCGGAGACCTCCAATTTCTCGCGGACATGCAAAATCTCGTCGTGCTTAACGTCTCGCACAACAACTTCACCGGACGCGTGCCGGACACGCCCTTCTTCGCGAAGCTCCCTCTCAGCGTCATGTCCGGCAACCCCTCCCTCTGCCTCACCGGCAGCCAGTGCGCTGAAGCAGGAAACCCCGGCTGGTCCCGCCGCCGCAACGCCGCCGCGCGCGTGGCGATGGTCGTCCTCCTCTGCACCGCCTGCACACTCCTCTTAGCGGCGCTCTACATCATCCTCGCAGCCCGAAAGCGGGCCCGGCCAGGGTTCTTCAGCGGGGCCCACGAACCCGATCCGGAGGACGACACCGAAGTCGACGTGGGCCCACCGTGGGAGGTGACGCTCTACCAGAAACTCGACCTGTCGATCGCCGACGTGGCGAAAAGCCTGACGCCTGACAACGTCATAGGCCGGGGGCGATCCGGCGTCGTTTACCACGTATTCCGTAACTCGGAGAAACACTCCGCCGCCGCTTTTTCCTCCGAGATTGCCACGCTGGCAAGGATACGTCACCGTAACATTGTCAGGCTACTGGGCTGGGGGGCCAACCGGAGAACGAAGTTACTGTTTTACGATTACTTGGCACAGGGTAATTTGGGCTCGTTGCTCCACGAGGGGTGCGCAGGATTAGTAGAGTGGGACACGCGGTTCAAGATAGCACTGGGCGTGGCGGAGGGTTTGGCTTATTTGCACCACGATTGCCAGCCGGCTATTTTGCACCGGGATGTGAAGGCCCAGAATATTTTGCTCGGGGATCAATACGAGGCCGTTTTGGCAGACTTTGGGCTCGCTAGGTTGGTTGAAGACGATCAAAACGGTCCGTTTTCGGCCAACCCCCAGTTTGCTGGTTCGTACGGCTACATTGCGCCTGAATATGCCTGTATGCTGAAAATTACTGCGAAAACTGATGTGTATAGCTATGGGGTAGTCCTTTTGGAGATCATAACTGGGAAAAGGCCAGCAGACCCCTCATTCCCGACGGCCAGCATCAAGAAGGATCCGATAGAGATTCTTGATCAGAAGCTCCAAGGCTACCCGGACACGCAGATACAAGAAATGCTCCAAGCGCTTGGAATTTCGCTCCTATGTACCAGCAACAGGGCGGAAGATCGTCCCACGATGAAGGATGTGGCTGCATTGTTGAGAGAAATTCGACATGATCAGCCCGCGACAGGAAGCGAGGCACACAAGCCTGCTAGCACCGGGTTGAAGAACTCATCTTCTTCCGTGACTCCAGCTCAACTGCTGATGATGCAAGGATCTTCTCATTGCTCACTTGCCTACTCTTCTTCAGCTGGTTATCTATCAGGAACCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1074

Amino Acids

116.32

Weight (kDa)

5.61

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 67 7.9e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 90 - 196 4.4e-11 Leucine-rich repeat region
LRR_8 PF13855 96 - 154 2.3e-07 Leucine rich repeat
LRR_8 PF13855 142 - 201 1e-06 Leucine rich repeat
LRR_14 PF23598 251 - 353 1.5e-06 Leucine-rich repeat region
LRR_8 PF13855 274 - 333 1.2e-07 Leucine rich repeat
LRR_8 PF13855 449 - 501 7e-06 Leucine rich repeat
LRR_14 PF23598 455 - 647 2e-10 Leucine-rich repeat region
LRR_8 PF13855 538 - 598 3e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 785 - 1014 1.8e-34 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 787 - 1012 1.2e-33 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013666)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05780 FvH4_5g05780 FvH4_5g05780
malus_domestica MD06G1170200.v1.1
prunus_persica Prupe.5G169700_v2.0.a1
pyrus_communis pycom06g15160
rosa_chinensis RchiOBHm_Chr7g0193041
rosa_laevigata RLG00000004301
rosa_multiflora Rmu_co8431803.1_g000001 Rmu_co8478551.1_g000001 Rmu_sc0002040.1_g000078
rosa_roxburghii Rroxscaffold_3G00262440
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132300 Rh7BG133200 Rh7CG136300 Rh7DG134200
rosa_wichuraiana Rw7G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 203
AasI GACNNNNNNGTC 1 cut(s) 2216
AatII GACGTC 1 cut(s) 1613
Acc16I TGCGCA 1 cut(s) 2527
Acc36I ACCTGC 2 cut(s) 203, 1494
AccB1I GGYRCC 1 cut(s) 256
AccB7I CCANNNNNTGG 2 cut(s) 704, 898
AccBSI CCGCTC 1 cut(s) 393
AccI GTMKAC 1 cut(s) 2220
AccII CGCG 8 cut(s) 1889, 1944, 1967, 2075, 2077, 2079, 2551, 3074
AccIII TCCGGA 2 cut(s) 1868, 2200
AcoI YGGCCR 4 cut(s) 1096, 2165, 2733, 2886
AcsI RAATTY 8 cut(s) 226, 265, 543, 919, 1091, 1402, 2979, 3054
AcuI CTGAAG 5 cut(s) 524, 953, 2052, 2161, 3180
AcyI GRCGYC 3 cut(s) 1610, 2297, 2331
AfaI GTAC 4 cut(s) 770, 1726, 2759, 2995
AfeI AGCGCT 1 cut(s) 2973
AflIII ACRYGT 2 cut(s) 909, 1942
AgeI ACCGGT 2 cut(s) 1217, 1641
AhdI GACNNNNNGTC 1 cut(s) 1587
AjiI CACGTC 2 cut(s) 2224, 2281
AjnI CCWGG 4 cut(s) 93, 260, 1591, 2167
AloI GAACNNNNNNTCC 6 cut(s) 1271, 1303, 1778, 1810, 2537, 2569
Alw21I GWGCWC 3 cut(s) 519, 879, 978
Alw26I GTCTC 5 cut(s) 125, 719, 1545, 1866, 1924
AlwNI CAGNNNCTG 1 cut(s) 1696
Ama87I CYCGRG 4 cut(s) 722, 1304, 1445, 2654
Aor13HI TCCGGA 2 cut(s) 1868, 2200
Aor51HI AGCGCT 1 cut(s) 2973
ApaI GGGCCC 3 cut(s) 2164, 2188, 2231
ApeKI GCWGC 7 cut(s) 421, 1018, 1375, 1461, 2019, 2147, 3038
ApoI RAATTY 8 cut(s) 226, 265, 543, 919, 1091, 1402, 2979, 3054
AsiGI ACCGGT 2 cut(s) 1217, 1641
Asp700I GAANNNNTTC 2 cut(s) 1318, 1814
AspLEI GCGC 8 cut(s) 1018, 2030, 2077, 2079, 2131, 2528, 2773, 2974
AsuII TTCGAA 1 cut(s) 1089
AsuNHI GCTAGC 1 cut(s) 3101
AvaI CYCGRG 4 cut(s) 722, 1304, 1445, 2654
AxyI CCTNAGG 1 cut(s) 135
BaeGI GKGCMC 4 cut(s) 261, 2164, 2188, 2231
BamHI GGATCC 1 cut(s) 2902
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 8 cut(s) 470, 879, 978, 2164, 2188, 2231, 2507, 2694
BarI GAAGNNNNNNTAC 2 cut(s) 849, 881
BauI CACGAG 2 cut(s) 333, 2516
BbsI GAAGAC 1 cut(s) 2715
Bbv12I GWGCWC 3 cut(s) 519, 879, 978
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 7 cut(s) 408, 1030, 1387, 1448, 2031, 2159, 3025
BccI CCATC 1 cut(s) 2080
BceAI ACGGC 3 cut(s) 2657, 2776, 2901
BciT130I CCWGG 4 cut(s) 95, 262, 1593, 2169
BciVI GTATCC 1 cut(s) 2403
BclI TGATCA 2 cut(s) 2920, 3064
BcoDI GTCTC 5 cut(s) 125, 719, 1545, 1866, 1924
BfaI CTAG 5 cut(s) 248, 549, 1022, 2697, 3102
BfmI CTRYAG 1 cut(s) 1518
BfoI RGCGCY 2 cut(s) 2132, 2975
BfuAI ACCTGC 2 cut(s) 203, 1494
BfuI GTATCC 1 cut(s) 2403
BglI GCCNNNNNGGC 4 cut(s) 2016, 2282, 2402, 2678
BglII AGATCT 1 cut(s) 411
BlpI GCTNAGC 3 cut(s) 143, 533, 664
BmeRI GACNNNNNGTC 1 cut(s) 1587
BmeT110I CYCGRG 4 cut(s) 722, 1304, 1445, 2654
BmgBI CACGTC 2 cut(s) 2224, 2281
BmrI ACTGGG 6 cut(s) 398, 470, 2447, 2576, 2737, 2862
BmsI GCATC 2 cut(s) 2901, 3147
BmtI GCTAGC 1 cut(s) 3105
BmuI ACTGGG 6 cut(s) 398, 470, 2447, 2576, 2737, 2862
BpiI GAAGAC 1 cut(s) 2715
BplI GAGNNNNNCTC 2 cut(s) 2351, 2383
BpmI CTGGAG 6 cut(s) 482, 612, 1232, 1273, 1529, 3123
Bpu10I CCTNAGC 4 cut(s) 374, 426, 446, 1778
Bpu1102I GCTNAGC 3 cut(s) 143, 533, 664
Bpu14I TTCGAA 1 cut(s) 1089
Bsa29I ATCGAT 1 cut(s) 1163
BsaAI YACGTR 1 cut(s) 2344
BsaHI GRCGYC 3 cut(s) 1610, 2297, 2331
BsaI GGTCTC 1 cut(s) 1866
BsaXI ACNNNNNCTCC 6 cut(s) 761, 791, 1271, 1301, 1778, 1808
Bse118I RCCGGY 9 cut(s) 320, 456, 1032, 1217, 1457, 1641, 1649, 2015, 2611
Bse21I CCTNAGG 1 cut(s) 135
Bse3DI GCAATG 2 cut(s) 2766, 3172
BseAI TCCGGA 2 cut(s) 1868, 2200
BseBI CCWGG 4 cut(s) 95, 262, 1593, 2169
BseCI ATCGAT 1 cut(s) 1163
BseGI GGATG 3 cut(s) 2139, 2635, 3037
BseMI GCAATG 2 cut(s) 2766, 3172
BsePI GCGCGC 1 cut(s) 2075
BseRI GAGGAG 4 cut(s) 701, 740, 2087, 2108
BseSI GKGCMC 4 cut(s) 261, 2164, 2188, 2231
BseX3I CGGCCG 1 cut(s) 1096
BseXI GCAGC 7 cut(s) 408, 1030, 1387, 1448, 2031, 2159, 3025
BseYI CCCAGC 1 cut(s) 2442
BsgI GTGCAG 3 cut(s) 625, 2086, 2095
Bsh1236I CGCG 8 cut(s) 1889, 1944, 1967, 2075, 2077, 2079, 2551, 3074
Bsh1285I CGRYCG 2 cut(s) 1099, 2274
BshNI GGYRCC 1 cut(s) 256
BshTI ACCGGT 2 cut(s) 1217, 1641
BshVI ATCGAT 1 cut(s) 1163
BsiEI CGRYCG 2 cut(s) 1099, 2274
BsiHKAI GWGCWC 3 cut(s) 519, 879, 978
BsiHKCI CYCGRG 4 cut(s) 722, 1304, 1445, 2654
BsiWI CGTACG 1 cut(s) 2757
BslFI GGGAC 7 cut(s) 167, 770, 1206, 1594, 2038, 2558, 3003
BsmAI GTCTC 5 cut(s) 125, 719, 1545, 1866, 1924
BsmBI CGTCTC 1 cut(s) 1924
BsmFI GGGAC 7 cut(s) 167, 770, 1206, 1594, 2038, 2558, 3003
BsmI GAATGC 3 cut(s) 936, 1489, 1839
Bso31I GGTCTC 1 cut(s) 1866
BsoBI CYCGRG 4 cut(s) 722, 1304, 1445, 2654
Bsp119I TTCGAA 1 cut(s) 1089
Bsp120I GGGCCC 3 cut(s) 2160, 2184, 2227
Bsp13I TCCGGA 2 cut(s) 1868, 2200
Bsp1720I GCTNAGC 3 cut(s) 143, 533, 664
Bsp19I CCATGG 1 cut(s) 14
Bsp68I TCGCGA 1 cut(s) 1967
BspDI ATCGAT 1 cut(s) 1163
BspEI TCCGGA 2 cut(s) 1868, 2200
BspFNI CGCG 8 cut(s) 1889, 1944, 1967, 2075, 2077, 2079, 2551, 3074
BspHI TCATGA 1 cut(s) 625
BspMAI CTGCAG 1 cut(s) 1522
BspMI ACCTGC 2 cut(s) 203, 1494
BspOI GCTAGC 1 cut(s) 3105
BspQI GCTCTTC 1 cut(s) 1388
BspT104I TTCGAA 1 cut(s) 1089
BspT107I GGYRCC 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 1866
BsrBI CCGCTC 1 cut(s) 393
BsrDI GCAATG 2 cut(s) 2766, 3172
BsrFI RCCGGY 9 cut(s) 320, 456, 1032, 1217, 1457, 1641, 1649, 2015, 2611
BssAI RCCGGY 9 cut(s) 320, 456, 1032, 1217, 1457, 1641, 1649, 2015, 2611
BssHII GCGCGC 1 cut(s) 2075
BssNI GRCGYC 3 cut(s) 1610, 2297, 2331
BssSI CACGAG 2 cut(s) 333, 2516
BssT1I CCWWGG 4 cut(s) 14, 703, 1702, 2932
Bst2BI CACGAG 2 cut(s) 333, 2516
Bst2UI CCWGG 4 cut(s) 95, 262, 1593, 2169
Bst4CI ACNGT 8 cut(s) 1175, 1275, 1319, 1692, 2235, 2420, 2472, 2723
Bst6I CTCTTC 5 cut(s) 32, 49, 1127, 1388, 3196
BstACI GRCGYC 3 cut(s) 1610, 2297, 2331
BstAPI GCANNNNNTGC 2 cut(s) 2025, 2109
BstBAI YACGTR 1 cut(s) 2344
BstBI TTCGAA 1 cut(s) 1089
BstDSI CCRYGG 2 cut(s) 14, 2234
BstEII GGTNACC 1 cut(s) 190
BstF5I GGATG 3 cut(s) 2139, 2635, 3037
BstFNI CGCG 8 cut(s) 1889, 1944, 1967, 2075, 2077, 2079, 2551, 3074
BstH2I RGCGCY 2 cut(s) 2132, 2975
BstHHI GCGC 8 cut(s) 1018, 2030, 2077, 2079, 2131, 2528, 2773, 2974
BstMAI GTCTC 5 cut(s) 125, 719, 1545, 1866, 1924
BstMCI CGRYCG 2 cut(s) 1099, 2274
BstNI CCWGG 4 cut(s) 95, 262, 1593, 2169
BstNSI RCATGY 2 cut(s) 913, 1897
BstPI GGTNACC 1 cut(s) 190
BstSFI CTRYAG 1 cut(s) 1518
BstSLI GKGCMC 4 cut(s) 261, 2164, 2188, 2231
BstUI CGCG 8 cut(s) 1889, 1944, 1967, 2075, 2077, 2079, 2551, 3074
BstV1I GCAGC 7 cut(s) 408, 1030, 1387, 1448, 2031, 2159, 3025
BstV2I GAAGAC 1 cut(s) 2715
BstX2I RGATCY 5 cut(s) 241, 411, 1008, 2902, 3164
BstXI CCANNNNNNTGG 3 cut(s) 180, 2750, 2975
BstYI RGATCY 5 cut(s) 241, 411, 1008, 2902, 3164
BstZI CGGCCG 1 cut(s) 1096
Bsu15I ATCGAT 1 cut(s) 1163
Bsu36I CCTNAGG 1 cut(s) 135
BsuI GTATCC 1 cut(s) 2403
BsuTUI ATCGAT 1 cut(s) 1163
BtgI CCRYGG 2 cut(s) 14, 2234
BtgZI GCGATG 1 cut(s) 2099
BtrI CACGTC 2 cut(s) 2224, 2281
BtsCI GGATG 3 cut(s) 2139, 2635, 3037
BtsIMutI CAGTG 5 cut(s) 365, 386, 1757, 2030, 2564
BtuMI TCGCGA 1 cut(s) 1967
BveI ACCTGC 2 cut(s) 203, 1494
CaiI CAGNNNCTG 1 cut(s) 1696
CciI TCATGA 1 cut(s) 625
CfoI GCGC 8 cut(s) 1018, 2030, 2077, 2079, 2131, 2528, 2773, 2974
Cfr10I RCCGGY 9 cut(s) 320, 456, 1032, 1217, 1457, 1641, 1649, 2015, 2611
ClaI ATCGAT 1 cut(s) 1163
CpoI CGGWCCG 1 cut(s) 2723
CseI GACGC 5 cut(s) 1950, 1972, 2254, 2305, 2320
Csp6I GTAC 4 cut(s) 769, 1725, 2758, 2994
CspAI ACCGGT 2 cut(s) 1217, 1641
CspI CGGWCCG 1 cut(s) 2723
CviAII CATG 9 cut(s) 15, 108, 174, 626, 655, 910, 1894, 1987, 3062
CviQI GTAC 4 cut(s) 769, 1725, 2758, 2994
DrdI GACNNNNNNGTC 1 cut(s) 2216
DriI GACNNNNNGTC 1 cut(s) 1587
DseDI GACNNNNNNGTC 1 cut(s) 2216
EaeI YGGCCR 4 cut(s) 1096, 2165, 2733, 2886
EagI CGGCCG 1 cut(s) 1096
Eam1104I CTCTTC 5 cut(s) 32, 49, 1127, 1388, 3196
Eam1105I GACNNNNNGTC 1 cut(s) 1587
EarI CTCTTC 5 cut(s) 32, 49, 1127, 1388, 3196
EciI GGCGGA 3 cut(s) 2359, 2591, 3023
Ecl136II GAGCTC 2 cut(s) 877, 976
EclXI CGGCCG 1 cut(s) 1096
Eco130I CCWWGG 4 cut(s) 14, 703, 1702, 2932
Eco24I GRGCYC 8 cut(s) 470, 879, 978, 2164, 2188, 2231, 2507, 2694
Eco31I GGTCTC 1 cut(s) 1866
Eco47III AGCGCT 1 cut(s) 2973
Eco52I CGGCCG 1 cut(s) 1096
Eco53kI GAGCTC 2 cut(s) 877, 976
Eco57I CTGAAG 5 cut(s) 524, 953, 2052, 2161, 3180
Eco81I CCTNAGG 1 cut(s) 135
Eco88I CYCGRG 4 cut(s) 722, 1304, 1445, 2654
Eco91I GGTNACC 1 cut(s) 190
EcoICRI GAGCTC 2 cut(s) 877, 976
EcoO109I RGGNCCY 3 cut(s) 154, 614, 2184
EcoO65I GGTNACC 1 cut(s) 190
EcoRI GAATTC 2 cut(s) 919, 1091
EcoRII CCWGG 4 cut(s) 93, 260, 1591, 2167
EcoT14I CCWWGG 4 cut(s) 14, 703, 1702, 2932
EcoT38I GRGCYC 8 cut(s) 470, 879, 978, 2164, 2188, 2231, 2507, 2694
ErhI CCWWGG 4 cut(s) 14, 703, 1702, 2932
Esp3I CGTCTC 1 cut(s) 1924
FaeI CATG 9 cut(s) 18, 111, 177, 629, 658, 913, 1897, 1990, 3065
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FaqI GGGAC 7 cut(s) 167, 770, 1206, 1594, 2038, 2558, 3003
FatI CATG 9 cut(s) 14, 107, 173, 625, 654, 909, 1893, 1986, 3061
FauI CCCGC 9 cut(s) 170, 530, 1308, 1380, 1700, 2063, 2151, 2174, 3079
FbaI TGATCA 2 cut(s) 2920, 3064
FblI GTMKAC 1 cut(s) 2220
FokI GGATG 3 cut(s) 2126, 2642, 3044
FriOI GRGCYC 8 cut(s) 470, 879, 978, 2164, 2188, 2231, 2507, 2694
FspBI CTAG 5 cut(s) 248, 549, 1022, 2697, 3102
FspI TGCGCA 1 cut(s) 2527
GlaI GCGC 8 cut(s) 1017, 2029, 2076, 2078, 2130, 2527, 2772, 2973
GsaI CCCAGC 1 cut(s) 2446
GsuI CTGGAG 6 cut(s) 482, 612, 1232, 1273, 1529, 3123
HaeII RGCGCY 2 cut(s) 2132, 2975
HgaI GACGC 5 cut(s) 1950, 1972, 2254, 2305, 2320
HhaI GCGC 8 cut(s) 1018, 2030, 2077, 2079, 2131, 2528, 2773, 2974
Hin1I GRCGYC 3 cut(s) 1610, 2297, 2331
Hin1II CATG 9 cut(s) 18, 111, 177, 629, 658, 913, 1897, 1990, 3065
Hin6I GCGC 8 cut(s) 1016, 2028, 2075, 2077, 2129, 2526, 2771, 2972
HinP1I GCGC 8 cut(s) 1016, 2028, 2075, 2077, 2129, 2526, 2771, 2972
HincII GTYRAC 2 cut(s) 1667, 2221
HindII GTYRAC 2 cut(s) 1667, 2221
HindIII AAGCTT 2 cut(s) 1139, 1262
Hpy166II GTNNAC 4 cut(s) 61, 1667, 2221, 2338
Hpy8I GTNNAC 4 cut(s) 61, 1667, 2221, 2338
Hpy99I CGWCG 6 cut(s) 1556, 1910, 2225, 2282, 2336, 2887
HpyCH4III ACNGT 8 cut(s) 1175, 1275, 1319, 1692, 2235, 2420, 2472, 2723
HpyCH4IV ACGT 9 cut(s) 1475, 1583, 1610, 1917, 2223, 2280, 2307, 2343, 2413
HpySE526I ACGT 9 cut(s) 1475, 1583, 1610, 1917, 2223, 2280, 2307, 2343, 2413
Hsp92I GRCGYC 3 cut(s) 1610, 2297, 2331
Hsp92II CATG 9 cut(s) 18, 111, 177, 629, 658, 913, 1897, 1990, 3065
HspAI GCGC 8 cut(s) 1016, 2028, 2075, 2077, 2129, 2526, 2771, 2972
KflI GGGWCCC 1 cut(s) 154
Kpn2I TCCGGA 2 cut(s) 1868, 2200
KroI GCCGGC 3 cut(s) 1032, 1457, 2611
KroNI GCCGGC 3 cut(s) 1034, 1459, 2613
Ksp22I TGATCA 2 cut(s) 2920, 3064
LguI GCTCTTC 1 cut(s) 1388
Lsp1109I GCAGC 7 cut(s) 408, 1030, 1387, 1448, 2031, 2159, 3025
LweI GCATC 2 cut(s) 2901, 3147
MaeI CTAG 5 cut(s) 248, 549, 1022, 2697, 3102
MaeII ACGT 9 cut(s) 1475, 1583, 1610, 1917, 2223, 2280, 2307, 2343, 2413
MauBI CGCGCGCG 1 cut(s) 2075
MbiI CCGCTC 1 cut(s) 393
MfeI CAATTG 3 cut(s) 148, 883, 980
MflI RGATCY 5 cut(s) 241, 411, 1008, 2902, 3164
MluI ACGCGT 1 cut(s) 1942
MlyI GAGTC 5 cut(s) 127, 1590, 1597, 1672, 3130
MmeI TCCRAC 1 cut(s) 1194
MroI TCCGGA 2 cut(s) 1868, 2200
MroNI GCCGGC 3 cut(s) 1032, 1457, 2611
MroXI GAANNNNTTC 2 cut(s) 1318, 1814
MseI TTAA 3 cut(s) 35, 351, 1914
MslI CAYNNNNRTG 4 cut(s) 178, 908, 914, 2628
MspA1I CMGCKG 3 cut(s) 537, 2181, 3200
MunI CAATTG 3 cut(s) 148, 883, 980
Mva1269I GAATGC 3 cut(s) 936, 1489, 1839
MvaI CCWGG 4 cut(s) 95, 262, 1593, 2169
MvnI CGCG 8 cut(s) 1889, 1944, 1967, 2075, 2077, 2079, 2551, 3074
NaeI GCCGGC 3 cut(s) 1034, 1459, 2613
NcoI CCATGG 1 cut(s) 14
NgoMIV GCCGGC 3 cut(s) 1032, 1457, 2611
NheI GCTAGC 1 cut(s) 3101
NlaIII CATG 9 cut(s) 18, 111, 177, 629, 658, 913, 1897, 1990, 3065
NmeAIII GCCGAG 2 cut(s) 858, 1815
NmuCI GTSAC 6 cut(s) 190, 887, 1611, 2242, 2414, 3133
NruI TCGCGA 1 cut(s) 1967
NsbI TGCGCA 1 cut(s) 2527
NspI RCATGY 2 cut(s) 913, 1897
NspV TTCGAA 1 cut(s) 1089
PaeR7I CTCGAG 1 cut(s) 722
PagI TCATGA 1 cut(s) 625
PaqCI CACCTGC 1 cut(s) 203
PauI GCGCGC 1 cut(s) 2075
PciI ACATGT 1 cut(s) 909
PciSI GCTCTTC 1 cut(s) 1388
PcsI WCGNNNNNNNCGW 3 cut(s) 1432, 1914, 2340
PctI GAATGC 3 cut(s) 936, 1489, 1839
PdiI GCCGGC 3 cut(s) 1034, 1459, 2613
PdmI GAANNNNTTC 2 cut(s) 1318, 1814
PfeI GAWTC 7 cut(s) 1081, 1252, 1330, 1423, 1468, 1750, 2914
Pfl23II CGTACG 1 cut(s) 2757
PflFI GACNNNGTC 1 cut(s) 2306
PflMI CCANNNNNTGG 2 cut(s) 704, 898
PfoI TCCNGGA 2 cut(s) 753, 1591
PinAI ACCGGT 2 cut(s) 1217, 1641
Ple19I CGATCG 1 cut(s) 2274
PleI GAGTC 5 cut(s) 126, 1590, 1596, 1671, 3130
PpsI GAGTC 5 cut(s) 126, 1590, 1596, 1671, 3130
Ppu21I YACGTR 1 cut(s) 2344
PpuMI RGGWCCY 2 cut(s) 154, 614
PscI ACATGT 1 cut(s) 909
Psp124BI GAGCTC 2 cut(s) 879, 978
Psp5II RGGWCCY 2 cut(s) 154, 614
Psp6I CCWGG 4 cut(s) 93, 260, 1591, 2167
PspEI GGTNACC 1 cut(s) 190
PspFI CCCAGC 1 cut(s) 2442
PspGI CCWGG 4 cut(s) 93, 260, 1591, 2167
PspLI CGTACG 1 cut(s) 2757
PspOMI GGGCCC 3 cut(s) 2160, 2184, 2227
PspPPI RGGWCCY 2 cut(s) 154, 614
PstI CTGCAG 1 cut(s) 1522
PstNI CAGNNNCTG 1 cut(s) 1696
PsuI RGATCY 5 cut(s) 241, 411, 1008, 2902, 3164
PsyI GACNNNGTC 1 cut(s) 2306
PteI GCGCGC 1 cut(s) 2075
PvuI CGATCG 1 cut(s) 2274
PvuII CAGCTG 1 cut(s) 3200
RruI TCGCGA 1 cut(s) 1967
RsaI GTAC 4 cut(s) 770, 1726, 2759, 2995
RsaNI GTAC 4 cut(s) 769, 1725, 2758, 2994
RseI CAYNNNNRTG 4 cut(s) 178, 908, 914, 2628
Rsr2I CGGWCCG 1 cut(s) 2723
RsrII CGGWCCG 1 cut(s) 2723
SacI GAGCTC 2 cut(s) 879, 978
SalI GTCGAC 1 cut(s) 2219
SapI GCTCTTC 1 cut(s) 1388
SaqAI TTAA 3 cut(s) 35, 351, 1914
SbfI CCTGCAGG 1 cut(s) 1522
SchI GAGTC 5 cut(s) 127, 1590, 1597, 1672, 3130
SdaI CCTGCAGG 1 cut(s) 1522
SfaNI GCATC 2 cut(s) 2901, 3147
SfcI CTRYAG 1 cut(s) 1518
Sfr274I CTCGAG 1 cut(s) 722
SfuI TTCGAA 1 cut(s) 1089
SlaI CTCGAG 1 cut(s) 722
SmiMI CAYNNNNRTG 4 cut(s) 178, 908, 914, 2628
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
Sse8387I CCTGCAGG 1 cut(s) 1522
SspI AATATT 1 cut(s) 2647
SspMI CTAG 5 cut(s) 248, 549, 1022, 2697, 3102
SstI GAGCTC 2 cut(s) 879, 978
StyI CCWWGG 4 cut(s) 14, 703, 1702, 2932
TaaI ACNGT 8 cut(s) 1175, 1275, 1319, 1692, 2235, 2420, 2472, 2723
TaiI ACGT 9 cut(s) 1478, 1586, 1613, 1920, 2226, 2283, 2310, 2346, 2416
TfiI GAWTC 7 cut(s) 1081, 1252, 1330, 1423, 1468, 1750, 2914
Tru1I TTAA 3 cut(s) 35, 351, 1914
Tru9I TTAA 3 cut(s) 35, 351, 1914
TscAI CASTG 5 cut(s) 372, 393, 1764, 2030, 2571
TseFI GTSAC 6 cut(s) 190, 887, 1611, 2242, 2414, 3133
TseI GCWGC 7 cut(s) 421, 1018, 1375, 1461, 2019, 2147, 3038
Tsp45I GTSAC 6 cut(s) 190, 887, 1611, 2242, 2414, 3133
TspDTI ATGAA 5 cut(s) 96, 180, 932, 1149, 3041
TspGWI ACGGA 3 cut(s) 2339, 2715, 3121
TspRI CASTG 5 cut(s) 372, 393, 1764, 2030, 2571
Tth111I GACNNNGTC 1 cut(s) 2306
Van91I CCANNNNNTGG 2 cut(s) 704, 898
XapI RAATTY 8 cut(s) 226, 265, 543, 919, 1091, 1402, 2979, 3054
XceI RCATGY 2 cut(s) 913, 1897
XcmI CCANNNNNNNNNTGG 1 cut(s) 1627
XhoI CTCGAG 1 cut(s) 722
XmiI GTMKAC 1 cut(s) 2220
XmnI GAANNNNTTC 2 cut(s) 1318, 1814
XspI CTAG 5 cut(s) 248, 549, 1022, 2697, 3102
ZraI GACGTC 1 cut(s) 1611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.