MD06G1170200.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013666
Physical Location & Seq
Forward (+)
31086860 .. 31091928
5069 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1170200.v1.1.491

Sequence Viewer

Length: 3360 bp
ATGCCTGTTCATCCATGGACGACCCTCCTCTCCTCCTCCTCCTTATTCATGTTGCTCCTCCTCTCTTTTTCTCTCGCAGCCTCGGCCTTAAACCCGCAAGGCCAAGCTCTGCTCTCGTGGAAGCAGAGCCTCAACGGCTCTGTTTCCACAAGTGCATTGGGAAACTGGGACCCGAATGACGAAGCGCCGTGCGGGTGGTTTGGGGTCACCTGCAACTCCAACGGCCAAGTTGTGGAGTTGAATTTGACGTACCTTGATTTGCTGGGTCAGCTCCCTTCTAACTTCACCTCATTGTCGACCATCACCAAGCTCACGCTCTCCGGCACCAACCTCACCGGCTCGATCCCGAAACAGATTTCCACCCTCAGAGACCTCACCGTACTGGACTTGAGCGACAATGCATTAACCGGCGAAATCCCAACCGAGATCTGCTCGCTGCCGAAGCTCGAGCAGCTTCACCTCAGCGCGAATAGATTGGAAGGCGAAATCCCAACCCAAATCGGCAACCTCACCAGCTTGAAATTGTTGGTTCTTTACGACAACCAGCTCAGCGGCAACGTTCCGAAATCGATTGGCGATTTGCACAATCTTGAAGTGATCAGAGCCGGCGGAAACAAGAACCTCGAAGGCCCTGTGCCCCACGAGATTGGAAGCTGCAACAATTTGGTAATGCTGGGATTGGCAGAAACCAGCATTTCGGGCTACCTCCCTCCATCTCTTGGCCTCCTCAAGAAACTCGAAACACTCGCAATCTACACCGCCTTGCTCTCAGGTCCGATCCCGCCGGAGCTTGGAGACTGCTCCGAGCTCCGGGACATCTATTTGTATGAGAATTCGATAACGGGATCGGTCCCGAGCCAATTGGGGAAGTTAAACAACCTTCAGAACCTTCTTCTCTGGCAGAACAACTTGGTGGGAGTCATTCCGCCGGAGCTCGGCAACTGCCTGCAGCTATTAGTCATCGACATCTCTATGAACTCATTGACTGGAATCATTCCTGTGTCTTTTGGAAACTTGACTTCGCTTCAGGAGCTGCAGCTGAGCGTCAATCAAATATCGGGCGAGATTCCGGCGCAGCTCGGAAACTGCCGGAAACTGACGCACATCGAGCTCGACAATAACCAGATCACCGGTTCGATACCGGCGGAGTTCGGAAACTTGTCGAATCTTTCTCTTCTTTTCTTGTGGCAGAACAAGCTCGAAGGAAACGTTCCGCCGTCGATTTCCAACTGTGTGAACCTCGAGTCCGTTGATTTGTCGCAGAACGGCTTGACGGGGCCGATTCCCGGAGGGCTCTTCAATCTCCAGAATCTCACGAAGCTTCTGCTGCTATCTAACAACTTCTCCGGTGAAATTCCGTCGGAGATCGGAAACTGCTCGTCGCTGATTCGGTTTCGGGCCAGCAGCAACAAACTCGCGGGAGGGATTCCGCCGCAGATTGGGAATTTGAGAAACTTGAATTTCTTGGATCTTGGATCGAATCGGCTTAGTCGAACCATACCGGAGGAGATCTCCGGCTGCCGGAATCTGACTTTTCTGGATTTGCATTCCAATTCTATTGGGGGAGATTTGCCTGGAGGTTTTGATAAGCTTGTATCTCTCCAATTCGTGGATTTCTCAGACAATTTGATCGAAGGGACGTTGAGACCTGGAATCGGATCGCTGAGTTCACTCACCAAGCTCGTTTTGGGAAATAATCGGTTCGCGGGTGCGATTCCGAGTGAACTCGGTTCATGCGCGAAGCTACAGTTGTTGGATTTGAGCGGGAACGAGCTGACAGGGAATATTCCGGCGAGCTTGGGCAAGATTCCCGCCCTGGAAATAGCTCTGAACCTGAGCTTGAATCAACTCTCCGGCGAGATACCGAAGGAGTTCGCCGATTTGGACAAGCTCGGGATACTAGACGTCTCTTACAATCAGCTCTCCGGCGATCTCCAATTTCTCGCGGACATGCAAAATCTAGTGGTCCTCAATATCTCGCACAACAACTTCTCGGGGAGAGTCCCAGACACGCCCTTCTTTTCGAAGCTTCCACTGAGCGTCTTGTCCGGCAATCCCTCGCTCTGTCTCTCCGGTGACAACCAGTGCTCCGCCGCGAACAAAAATCCCAACGGATCCCGCCGCCGCAATGTAGCAGCGCGCGTGGCCATGGTCGTGCTACTGTGCACCGCTTGCACGCTTCTTCTTACGGCGTTCTACATTATCCTCGGAGCAAAGATGCGGGGCCCGGCCGGACTCTTCGGACGGTCCCATGAGCCCGACCCGGAGGACGACAGCGAGTTGGACGTGGGCCCACCGTGGGAGGTTACGCTTTACCAGAAGCTCGACCTGTCGATTGTCGACGTGGCGCGAAGTTTAACTCCTTGCAACGTCATCGGACGGGGCCGCTCTGGCGTCGTTTATCAAGTCACCATCCCCTCAGGCTTATCCATCGCAGTAAAAAGATTCCGTCCGTCGGAGAAATACTCGGCCTCCGCATTTTCCTCAGAGATTGCCACGTTGGCAAGAATACGACACCGTAACATCGTACGGTTACTCGGTTGGGGCGCGAACCGGAGGACGAAGTTACTGTTTTACGACCATCTGGCCAACGGTAATCTGGGTTCGTTACTGCACGAGGGGTGCGCAGGATTAGTGGAGTGGGACACCCGGTTCAGGATAGCGTCCGGCGTGGCGGAGGGGTTGGCGTACTTGCACCACGATTGCCAGCCGGCTATCTTGCACCGCGACGTGAAGGCGCAAAACATTCTGCTGGGGGATCGATACGAGGCCGTTTTGGCGGACTTTGGACTTGCTAGGCTGGTGGAGGATGACTCAAACGGTCCGTTTTCAGCCAACCCCCAATTTGCCGGGTCCTACGGCTACATTGCGCCGGAATATGCATGCATGCTAAAAATCACTGCGAAAAGCGATGTGTATAGTTATGGGGTTGTCCTTTTGGAGATTATAACTGGGAAAAGGCCAGTGGACCCATCATTCACAGATGGGCAACATGTGATTCAGTGGGTCCGAGACCACTTGAAGAGCAAGAAGGACCCTGTTGAGATTCTTGATCCTAAACTTCAAGGTTACCCAGACGCACAGATACAAGAAATGCTCCAAGCTCTTGGAATCTCACTCCTCTGCACCAGCAACCGGGTGGATGATCGTCCCACGATGAAGGACGTGGCGGCATTGTTGAGAGAAATTCAACACGATCAGGGCCCGATGGGAGGCGAGGTGCATAAGCTGGCTACCAACGCGTTGAAGAATTCATCGTCTTCTGTGACACCGGCTCAATTGCTTCAGCTGCAAGGCTCCTCTCATTGCTCACTTGCTTACTCATCTGCTTCAGCTGGTTATCTGTCGGGAACTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1120

Amino Acids

120.29

Weight (kDa)

5.41

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 72 6.3e-11 Leucine rich repeat N-terminal domain
LRR_14 PF23598 98 - 201 3.5e-12 Leucine-rich repeat region
LRR_8 PF13855 100 - 159 4.6e-09 Leucine rich repeat
LRR_14 PF23598 264 - 372 4.2e-08 Leucine-rich repeat region
LRR_8 PF13855 293 - 352 1.3e-07 Leucine rich repeat
LRR_14 PF23598 475 - 667 2.8e-10 Leucine-rich repeat region
LRR_8 PF13855 484 - 544 4.2e-06 Leucine rich repeat
Pkinase PF00069 790 - 1056 3.4e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 790 - 1056 1.5e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013666)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05780 FvH4_5g05780 FvH4_5g05780
malus_domestica MD06G1170200.v1.1
prunus_persica Prupe.5G169700_v2.0.a1
pyrus_communis pycom06g15160
rosa_chinensis RchiOBHm_Chr7g0193041
rosa_laevigata RLG00000004301
rosa_multiflora Rmu_co8431803.1_g000001 Rmu_co8478551.1_g000001 Rmu_sc0002040.1_g000078
rosa_roxburghii Rroxscaffold_3G00262440
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132300 Rh7BG133200 Rh7CG136300 Rh7DG134200
rosa_wichuraiana Rw7G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2948
AarI CACCTGC 1 cut(s) 218
AatII GACGTC 1 cut(s) 1908
Acc16I TGCGCA 1 cut(s) 2626
Acc36I ACCTGC 1 cut(s) 218
AccB1I GGYRCC 1 cut(s) 323
AccB7I CCANNNNNTGG 3 cut(s) 232, 719, 1609
AccBSI CCGCTC 2 cut(s) 1764, 2388
AccI GTMKAC 2 cut(s) 296, 2340
AclI AACGTT 2 cut(s) 558, 1209
AcoI YGGCCR 4 cut(s) 223, 2145, 2229, 2586
AcsI RAATTY 7 cut(s) 241, 832, 1353, 1444, 1459, 3186, 3250
AcuI CTGAAG 4 cut(s) 866, 1010, 3269, 3315
AcyI GRCGYC 2 cut(s) 1905, 2394
AfaI GTAC 4 cut(s) 251, 381, 2529, 2690
AflIII ACRYGT 2 cut(s) 2992, 3240
AgeI ACCGGT 1 cut(s) 1130
AjiI CACGTC 4 cut(s) 2287, 2344, 2731, 3166
AjnI CCWGG 3 cut(s) 1573, 1648, 1815
AloI GAACNNNNNNTCC 4 cut(s) 1229, 1261, 2636, 2668
Alw21I GWGCWC 5 cut(s) 810, 936, 1113, 2090, 2168
Alw26I GTCTC 6 cut(s) 363, 789, 1639, 1912, 2072, 3006
Alw44I GTGCAC 1 cut(s) 2164
AlwNI CAGNNNCTG 1 cut(s) 1033
Ama87I CYCGRG 5 cut(s) 446, 853, 1241, 1892, 1993
ApaI GGGCCC 3 cut(s) 2228, 2294, 3206
ApaLI GTGCAC 1 cut(s) 2164
ApoI RAATTY 7 cut(s) 241, 832, 1353, 1444, 1459, 3186, 3250
AsiGI ACCGGT 1 cut(s) 1130
Asp700I GAANNNNTTC 1 cut(s) 1871
AsuC2I CCSGG 7 cut(s) 812, 1287, 2228, 2264, 2650, 2851, 3137
AsuII TTCGAA 1 cut(s) 2024
AvaI CYCGRG 5 cut(s) 446, 853, 1241, 1892, 1993
AxyI CCTNAGG 1 cut(s) 2419
BaeGI GKGCMC 5 cut(s) 639, 2168, 2228, 2294, 3206
BalI TGGCCA 2 cut(s) 2147, 2588
BamHI GGATCC 1 cut(s) 2114
BanI GGYRCC 1 cut(s) 323
BanII GRGCYC 8 cut(s) 810, 936, 1113, 1296, 2228, 2259, 2294, 3206
BauI CACGAG 3 cut(s) 115, 641, 2615
BbsI GAAGAC 1 cut(s) 3252
Bbv12I GWGCWC 5 cut(s) 810, 936, 1113, 2090, 2168
BbvCI CCTCAGC 1 cut(s) 461
BccI CCATC 8 cut(s) 308, 721, 2420, 2438, 2589, 2978, 2980, 3202
BceAI ACGGC 8 cut(s) 151, 172, 238, 1201, 1282, 2205, 2756, 2875
BcgI CGANNNNNNTGC 6 cut(s) 1306, 1340, 1396, 1430, 2356, 2390
BciT130I CCWGG 3 cut(s) 1575, 1650, 1817
BciVI GTATCC 1 cut(s) 1890
BclI TGATCA 1 cut(s) 597
BcnI CCSGG 7 cut(s) 812, 1287, 2228, 2264, 2650, 2851, 3137
BcoDI GTCTC 6 cut(s) 363, 789, 1639, 1912, 2072, 3006
BfaI CTAG 3 cut(s) 1901, 1961, 2796
BfmI CTRYAG 3 cut(s) 947, 1034, 1745
BfoI RGCGCY 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 218
BfuI GTATCC 1 cut(s) 1890
BglI GCCNNNNNGGC 4 cut(s) 135, 2391, 2501, 2777
BglII AGATCT 2 cut(s) 426, 1509
BlpI GCTNAGC 2 cut(s) 548, 1040
BmeT110I CYCGRG 5 cut(s) 446, 853, 1241, 1892, 1993
BmgBI CACGTC 4 cut(s) 2287, 2344, 2731, 3166
BmrI ACTGGG 2 cut(s) 175, 2961
BmsI GCATC 1 cut(s) 2208
BmuI ACTGGG 2 cut(s) 175, 2961
BpiI GAAGAC 1 cut(s) 3252
BplI GAGNNNNNCTC 8 cut(s) 416, 448, 1496, 1528, 2450, 2482, 2798, 2830
BpmI CTGGAG 2 cut(s) 1289, 1596
Bpu10I CCTNAGC 2 cut(s) 461, 1835
Bpu1102I GCTNAGC 2 cut(s) 548, 1040
Bpu14I TTCGAA 1 cut(s) 2024
BpuEI CTTGAG 2 cut(s) 409, 713
BpuMI CCSGG 7 cut(s) 812, 1287, 2228, 2264, 2650, 2851, 3137
Bsa29I ATCGAT 2 cut(s) 569, 2761
BsaHI GRCGYC 2 cut(s) 1905, 2394
BsaI GGTCTC 3 cut(s) 363, 1639, 3006
BsaJI CCNNGG 6 cut(s) 14, 81, 1815, 2148, 2206, 2297
BsaWI WCCGGW 5 cut(s) 1130, 1346, 1501, 2072, 2553
BsaXI ACNNNNNCTCC 8 cut(s) 1328, 1358, 2072, 2102, 2456, 2486, 3204, 3234
Bse118I RCCGGY 7 cut(s) 335, 407, 605, 1130, 1141, 2710, 3271
Bse1I ACTGG 6 cut(s) 170, 387, 991, 2083, 2956, 2963
Bse21I CCTNAGG 1 cut(s) 2419
Bse3DI GCAATG 3 cut(s) 2134, 2865, 3304
BseBI CCWGG 3 cut(s) 1575, 1650, 1817
BseCI ATCGAT 2 cut(s) 569, 2761
BseDI CCNNGG 6 cut(s) 14, 81, 1815, 2148, 2206, 2297
BseGI GGATG 4 cut(s) 10, 2412, 2815, 3148
BseMI GCAATG 3 cut(s) 2134, 2865, 3304
BseNI ACTGG 6 cut(s) 170, 387, 991, 2083, 2956, 2963
BsePI GCGCGC 1 cut(s) 2138
BseSI GKGCMC 5 cut(s) 639, 2168, 2228, 2294, 3206
BseX3I CGGCCG 1 cut(s) 2229
BseYI CCCAGC 3 cut(s) 262, 673, 2752
BsgI GTGCAG 2 cut(s) 2597, 3109
Bsh1285I CGRYCG 1 cut(s) 2232
BshNI GGYRCC 1 cut(s) 323
BshTI ACCGGT 1 cut(s) 1130
BshVI ATCGAT 2 cut(s) 569, 2761
BsiEI CGRYCG 1 cut(s) 2232
BsiHKAI GWGCWC 5 cut(s) 810, 936, 1113, 2090, 2168
BsiHKCI CYCGRG 5 cut(s) 446, 853, 1241, 1892, 1993
BsiWI CGTACG 1 cut(s) 2527
BslFI GGGAC 8 cut(s) 182, 827, 836, 1651, 1988, 2233, 2657, 3135
BsmAI GTCTC 6 cut(s) 363, 789, 1639, 1912, 2072, 3006
BsmBI CGTCTC 1 cut(s) 1912
BsmFI GGGAC 8 cut(s) 182, 827, 836, 1651, 1988, 2233, 2657, 3135
BsmI GAATGC 1 cut(s) 1546
Bso31I GGTCTC 3 cut(s) 363, 1639, 3006
BsoBI CYCGRG 5 cut(s) 446, 853, 1241, 1892, 1993
Bsp119I TTCGAA 1 cut(s) 2024
Bsp120I GGGCCC 3 cut(s) 2224, 2290, 3202
Bsp1720I GCTNAGC 2 cut(s) 548, 1040
Bsp19I CCATGG 2 cut(s) 14, 2148
BspDI ATCGAT 2 cut(s) 569, 2761
BspMAI CTGCAG 2 cut(s) 951, 1038
BspMI ACCTGC 1 cut(s) 218
BspQI GCTCTTC 2 cut(s) 1301, 3017
BspT104I TTCGAA 1 cut(s) 2024
BspT107I GGYRCC 1 cut(s) 323
BspTNI GGTCTC 3 cut(s) 363, 1639, 3006
BsrBI CCGCTC 2 cut(s) 1764, 2388
BsrDI GCAATG 3 cut(s) 2134, 2865, 3304
BsrFI RCCGGY 7 cut(s) 335, 407, 605, 1130, 1141, 2710, 3271
BsrI ACTGG 6 cut(s) 170, 387, 991, 2083, 2956, 2963
BssAI RCCGGY 7 cut(s) 335, 407, 605, 1130, 1141, 2710, 3271
BssECI CCNNGG 6 cut(s) 14, 81, 1815, 2148, 2206, 2297
BssHII GCGCGC 1 cut(s) 2138
BssNI GRCGYC 2 cut(s) 1905, 2394
BssSI CACGAG 3 cut(s) 115, 641, 2615
BssT1I CCWWGG 2 cut(s) 14, 2148
Bst2BI CACGAG 3 cut(s) 115, 641, 2615
Bst2UI CCWGG 3 cut(s) 1575, 1650, 1817
Bst6I CTCTTC 4 cut(s) 1179, 1301, 2243, 3017
BstACI GRCGYC 2 cut(s) 1905, 2394
BstAPI GCANNNNNTGC 1 cut(s) 2172
BstBI TTCGAA 1 cut(s) 2024
BstDSI CCRYGG 3 cut(s) 14, 2148, 2297
BstEII GGTNACC 2 cut(s) 205, 3068
BstF5I GGATG 4 cut(s) 10, 2412, 2815, 3148
BstH2I RGCGCY 1 cut(s) 188
BstMAI GTCTC 6 cut(s) 363, 789, 1639, 1912, 2072, 3006
BstMCI CGRYCG 1 cut(s) 2232
BstNI CCWGG 3 cut(s) 1575, 1650, 1817
BstNSI RCATGY 4 cut(s) 1954, 2886, 2890, 2996
BstPI GGTNACC 2 cut(s) 205, 3068
BstSFI CTRYAG 3 cut(s) 947, 1034, 1745
BstSLI GKGCMC 5 cut(s) 639, 2168, 2228, 2294, 3206
BstV2I GAAGAC 1 cut(s) 3252
BstX2I RGATCY 4 cut(s) 426, 1468, 1509, 2114
BstXI CCANNNNNNTGG 2 cut(s) 647, 3107
BstYI RGATCY 4 cut(s) 426, 1468, 1509, 2114
BstZI CGGCCG 1 cut(s) 2229
Bsu15I ATCGAT 2 cut(s) 569, 2761
Bsu36I CCTNAGG 1 cut(s) 2419
BsuI GTATCC 1 cut(s) 1890
BsuTUI ATCGAT 2 cut(s) 569, 2761
BtgI CCRYGG 3 cut(s) 14, 2148, 2297
BtgZI GCGATG 2 cut(s) 2416, 2925
BtrI CACGTC 4 cut(s) 2287, 2344, 2731, 3166
BtsCI GGATG 4 cut(s) 10, 2412, 2815, 3148
BtsI GCAGTG 1 cut(s) 2898
BtsIMutI CAGTG 5 cut(s) 2033, 2090, 2898, 2970, 3008
BveI ACCTGC 1 cut(s) 218
CaiI CAGNNNCTG 1 cut(s) 1033
Cfr10I RCCGGY 7 cut(s) 335, 407, 605, 1130, 1141, 2710, 3271
ClaI ATCGAT 2 cut(s) 569, 2761
CpoI CGGWCCG 1 cut(s) 2822
CseI GACGC 6 cut(s) 1033, 1108, 2029, 2383, 2652, 3086
Csp6I GTAC 4 cut(s) 250, 380, 2528, 2689
CspAI ACCGGT 1 cut(s) 1130
CspI CGGWCCG 1 cut(s) 2822
CviAII CATG 9 cut(s) 15, 49, 1734, 1951, 2149, 2252, 2883, 2887, 2993
CviQI GTAC 4 cut(s) 250, 380, 2528, 2689
EaeI YGGCCR 4 cut(s) 223, 2145, 2229, 2586
EagI CGGCCG 1 cut(s) 2229
Eam1104I CTCTTC 4 cut(s) 1179, 1301, 2243, 3017
EarI CTCTTC 4 cut(s) 1179, 1301, 2243, 3017
EciI GGCGGA 8 cut(s) 624, 915, 1160, 1203, 1419, 2080, 2690, 2795
Ecl136II GAGCTC 3 cut(s) 808, 934, 1111
EclXI CGGCCG 1 cut(s) 2229
Eco130I CCWWGG 2 cut(s) 14, 2148
Eco24I GRGCYC 8 cut(s) 810, 936, 1113, 1296, 2228, 2259, 2294, 3206
Eco31I GGTCTC 3 cut(s) 363, 1639, 3006
Eco52I CGGCCG 1 cut(s) 2229
Eco53kI GAGCTC 3 cut(s) 808, 934, 1111
Eco57I CTGAAG 4 cut(s) 866, 1010, 3269, 3315
Eco81I CCTNAGG 1 cut(s) 2419
Eco88I CYCGRG 5 cut(s) 446, 853, 1241, 1892, 1993
Eco91I GGTNACC 2 cut(s) 205, 3068
EcoICRI GAGCTC 3 cut(s) 808, 934, 1111
EcoO109I RGGNCCY 6 cut(s) 169, 629, 2224, 2853, 3034, 3202
EcoO65I GGTNACC 2 cut(s) 205, 3068
EcoRI GAATTC 2 cut(s) 832, 3250
EcoRII CCWGG 3 cut(s) 1573, 1648, 1815
EcoT14I CCWWGG 2 cut(s) 14, 2148
EcoT22I ATGCAT 3 cut(s) 403, 2884, 2888
EcoT38I GRGCYC 8 cut(s) 810, 936, 1113, 1296, 2228, 2259, 2294, 3206
ErhI CCWWGG 2 cut(s) 14, 2148
Esp3I CGTCTC 1 cut(s) 1912
FaeI CATG 9 cut(s) 18, 52, 1737, 1954, 2152, 2255, 2886, 2890, 2996
FaqI GGGAC 8 cut(s) 182, 827, 836, 1651, 1988, 2233, 2657, 3135
FatI CATG 9 cut(s) 14, 48, 1733, 1950, 2148, 2251, 2882, 2886, 2992
FauI CCCGC 9 cut(s) 102, 185, 789, 1411, 1699, 1757, 1819, 2126, 2214
FbaI TGATCA 1 cut(s) 597
FblI GTMKAC 2 cut(s) 296, 2340
FokI GGATG 3 cut(s) 2399, 2822, 3155
FriOI GRGCYC 8 cut(s) 810, 936, 1113, 1296, 2228, 2259, 2294, 3206
FspBI CTAG 3 cut(s) 1901, 1961, 2796
FspI TGCGCA 1 cut(s) 2626
GsaI CCCAGC 3 cut(s) 266, 677, 2756
GsuI CTGGAG 2 cut(s) 1289, 1596
HaeII RGCGCY 1 cut(s) 188
HgaI GACGC 6 cut(s) 1033, 1108, 2029, 2383, 2652, 3086
Hin1I GRCGYC 2 cut(s) 1905, 2394
Hin1II CATG 9 cut(s) 18, 52, 1737, 1954, 2152, 2255, 2886, 2890, 2996
HincII GTYRAC 2 cut(s) 297, 2341
HindII GTYRAC 2 cut(s) 297, 2341
HindIII AAGCTT 3 cut(s) 1319, 1589, 2027
Hpy166II GTNNAC 7 cut(s) 297, 1237, 1670, 1724, 2166, 2341, 2968
Hpy8I GTNNAC 7 cut(s) 297, 1237, 1670, 1724, 2166, 2341, 2968
Hpy99I CGWCG 7 cut(s) 1222, 1363, 1384, 2345, 2399, 2458, 2732
Hsp92I GRCGYC 2 cut(s) 1905, 2394
Hsp92II CATG 9 cut(s) 18, 52, 1737, 1954, 2152, 2255, 2886, 2890, 2996
KflI GGGWCCC 1 cut(s) 169
KroI GCCGGC 2 cut(s) 605, 2710
KroNI GCCGGC 2 cut(s) 607, 2712
Ksp22I TGATCA 1 cut(s) 597
LguI GCTCTTC 2 cut(s) 1301, 3017
LweI GCATC 1 cut(s) 2208
MaeI CTAG 3 cut(s) 1901, 1961, 2796
MbiI CCGCTC 2 cut(s) 1764, 2388
MboII GAAGA 8 cut(s) 884, 1166, 1288, 2174, 2230, 3034, 3252, 3259
MfeI CAATTG 2 cut(s) 860, 3278
MflI RGATCY 4 cut(s) 426, 1468, 1509, 2114
MlsI TGGCCA 2 cut(s) 2147, 2588
MluI ACGCGT 1 cut(s) 3240
MluNI TGGCCA 2 cut(s) 2147, 2588
MlyI GAGTC 5 cut(s) 927, 1253, 2010, 2229, 2807
MmeI TCCRAC 6 cut(s) 243, 1251, 1341, 1734, 2262, 2436
Mox20I TGGCCA 2 cut(s) 2147, 2588
Mph1103I ATGCAT 3 cut(s) 403, 2884, 2888
MroNI GCCGGC 2 cut(s) 605, 2710
MroXI GAANNNNTTC 1 cut(s) 1871
MscI TGGCCA 2 cut(s) 2147, 2588
MseI TTAA 4 cut(s) 89, 404, 872, 2357
MslI CAYNNNNRTG 3 cut(s) 971, 998, 2153
Msp20I TGGCCA 2 cut(s) 2147, 2588
MspA1I CMGCKG 4 cut(s) 552, 1039, 3289, 3335
MunI CAATTG 2 cut(s) 860, 3278
Mva1269I GAATGC 1 cut(s) 1546
MvaI CCWGG 3 cut(s) 1575, 1650, 1817
NaeI GCCGGC 2 cut(s) 607, 2712
NciI CCSGG 7 cut(s) 812, 1287, 2228, 2264, 2650, 2851, 3137
NcoI CCATGG 2 cut(s) 14, 2148
NgoMIV GCCGGC 2 cut(s) 605, 2710
NlaIII CATG 9 cut(s) 18, 52, 1737, 1954, 2152, 2255, 2886, 2890, 2996
NmeAIII GCCGAG 3 cut(s) 62, 915, 2447
NmuCI GTSAC 4 cut(s) 205, 2075, 2407, 3265
NsbI TGCGCA 1 cut(s) 2626
NsiI ATGCAT 3 cut(s) 403, 2884, 2888
NspI RCATGY 4 cut(s) 1954, 2886, 2890, 2996
NspV TTCGAA 1 cut(s) 2024
PaeI GCATGC 2 cut(s) 2886, 2890
PaeR7I CTCGAG 2 cut(s) 446, 1241
PaqCI CACCTGC 1 cut(s) 218
PauI GCGCGC 1 cut(s) 2138
PciI ACATGT 1 cut(s) 2992
PciSI GCTCTTC 2 cut(s) 1301, 3017
PcsI WCGNNNNNNNCGW 2 cut(s) 1206, 1489
PctI GAATGC 1 cut(s) 1546
PdiI GCCGGC 2 cut(s) 607, 2712
PdmI GAANNNNTTC 1 cut(s) 1871
Pfl23II CGTACG 1 cut(s) 2527
PflMI CCANNNNNTGG 3 cut(s) 232, 719, 1609
PfoI TCCNGGA 2 cut(s) 810, 1285
PinAI ACCGGT 1 cut(s) 1130
PleI GAGTC 5 cut(s) 926, 1252, 2009, 2229, 2807
PpsI GAGTC 5 cut(s) 926, 1252, 2009, 2229, 2807
PpuMI RGGWCCY 3 cut(s) 169, 2853, 3034
PscI ACATGT 1 cut(s) 2992
PsiI TTATAA 1 cut(s) 2948
Psp124BI GAGCTC 3 cut(s) 810, 936, 1113
Psp1406I AACGTT 2 cut(s) 558, 1209
Psp5II RGGWCCY 3 cut(s) 169, 2853, 3034
Psp6I CCWGG 3 cut(s) 1573, 1648, 1815
PspEI GGTNACC 2 cut(s) 205, 3068
PspFI CCCAGC 3 cut(s) 262, 673, 2752
PspGI CCWGG 3 cut(s) 1573, 1648, 1815
PspLI CGTACG 1 cut(s) 2527
PspOMI GGGCCC 3 cut(s) 2224, 2290, 3202
PspPPI RGGWCCY 3 cut(s) 169, 2853, 3034
PspXI VCTCGAGB 2 cut(s) 446, 1241
PsrI GAACNNNNNNTAC 2 cut(s) 2587, 2619
PstI CTGCAG 2 cut(s) 951, 1038
PstNI CAGNNNCTG 1 cut(s) 1033
PsuI RGATCY 4 cut(s) 426, 1468, 1509, 2114
PteI GCGCGC 1 cut(s) 2138
PvuII CAGCTG 3 cut(s) 1039, 3289, 3335
RsaI GTAC 4 cut(s) 251, 381, 2529, 2690
RsaNI GTAC 4 cut(s) 250, 380, 2528, 2689
RseI CAYNNNNRTG 3 cut(s) 971, 998, 2153
Rsr2I CGGWCCG 1 cut(s) 2822
RsrII CGGWCCG 1 cut(s) 2822
SacI GAGCTC 3 cut(s) 810, 936, 1113
SalI GTCGAC 2 cut(s) 295, 2339
SapI GCTCTTC 2 cut(s) 1301, 3017
SaqAI TTAA 4 cut(s) 89, 404, 872, 2357
SchI GAGTC 5 cut(s) 927, 1253, 2010, 2229, 2807
SfaNI GCATC 1 cut(s) 2208
SfcI CTRYAG 3 cut(s) 947, 1034, 1745
Sfr274I CTCGAG 2 cut(s) 446, 1241
SfuI TTCGAA 1 cut(s) 2024
SlaI CTCGAG 2 cut(s) 446, 1241
SmiMI CAYNNNNRTG 3 cut(s) 971, 998, 2153
SmlI CTYRAG 4 cut(s) 388, 446, 728, 1241
SmoI CTYRAG 4 cut(s) 388, 446, 728, 1241
SphI GCATGC 2 cut(s) 2886, 2890
SspI AATATT 1 cut(s) 1786
SspMI CTAG 3 cut(s) 1901, 1961, 2796
SstI GAGCTC 3 cut(s) 810, 936, 1113
StyI CCWWGG 2 cut(s) 14, 2148
TaqII GACCGA 1 cut(s) 838
TauI GCSGC 7 cut(s) 555, 1435, 2096, 2124, 2127, 2388, 3173
Tru1I TTAA 4 cut(s) 89, 404, 872, 2357
Tru9I TTAA 4 cut(s) 89, 404, 872, 2357
TscAI CASTG 5 cut(s) 2040, 2090, 2905, 2970, 3008
TseFI GTSAC 4 cut(s) 205, 2075, 2407, 3265
Tsp45I GTSAC 4 cut(s) 205, 2075, 2407, 3265
TspDTI ATGAA 5 cut(s) 37, 989, 1722, 3173, 3243
TspGWI ACGGA 6 cut(s) 1237, 1347, 2127, 2438, 2442, 2814
TspRI CASTG 5 cut(s) 2040, 2090, 2905, 2970, 3008
Van91I CCANNNNNTGG 3 cut(s) 232, 719, 1609
VneI GTGCAC 1 cut(s) 2164
XapI RAATTY 7 cut(s) 241, 832, 1353, 1444, 1459, 3186, 3250
XceI RCATGY 4 cut(s) 1954, 2886, 2890, 2996
XcmI CCANNNNNNNNNTGG 4 cut(s) 154, 1684, 2596, 3136
XhoI CTCGAG 2 cut(s) 446, 1241
XmiI GTMKAC 2 cut(s) 296, 2340
XmnI GAANNNNTTC 1 cut(s) 1871
XspI CTAG 3 cut(s) 1901, 1961, 2796
ZraI GACGTC 1 cut(s) 1906
Zsp2I ATGCAT 3 cut(s) 403, 2884, 2888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.