Rroxscaffold_3G00262440

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013666
Physical Location & Seq
Reverse (-)
56433407 .. 56439735
6329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262440.1

Sequence Viewer

Length: 3525 bp
ATGCCTGTAAATCCATGGACCTTCCTCTTCTTCTTAACCCTCTCTTCTATTCTTCTGGTTCACTTTCCTGCTCTGGCCTTGAATCAACAAGGCCAGGCTCTTGTTTCATGGAAGCAGAGTCTCAATGGCTCCCCTGAGGGGCTGAGCAATTGGGACCCAAGTGATGAAACTCCATGCGGGTGGTCCGGGGTCACCTGCAACTTCAACAACCAAGTTGTGGAGCTGAATTTGAAGTATTTTGATCTTCTAGGTAAGGTGCCCTCGAATTTCACTTCGTTGCTGACCCTAAACAAGCTCATCTTGTCCGGCACAAACCTCACCGGCTCAATCCCACGAGAAATCTCTACTCTTAGACAACTGACCTCACTGGACCTGAGCGACAATGCCCTGAGCGGCGAAATCCCAGTTGAGATCTGCGAGCTGCCTAAGCTCGAAGAACTCTACCTCAGCACGAACCGGCTGGAGGGCTCAATCCCAGTCCAAATCGGAAACCTCACAAACCTGAAGTGGCTGGTTCTCTACGACAATCAGCTCAGCGGGAAAATTCCTAGCACCATTGGAAATCTGCCGCAACTTCAAGTAATCAGAGCTGGAGGGAACAAGAACCTTGAAGGTCCTGTGCCCCATGAGATTGGGAACTGCACCGATTTGGTCATGTTGGGATTAGCAGAAACCAGCATTTCCGGCTTCCTCCCTCCAAGCCTTGGCCTCCTCAAAAAACTCGAGACATTGGCAATCTACACCACCCTCGTCTCCGAGCTCGGAGACTGCACCGAGCTCCGGGACATCTACTTTTACGAAAACTCCATTAGCGGCTCTGTTCCGAGCAAATTAGGCAACTTGAAAAATCTTCAGAACCTTCTGCTATGGCAAAACAGCCTTGTAGGGGTTATTCCACCGGAGCTCGGCAATTGTCACCAGCTATTGGTCATTGACATTTCTATGAATTCCTTGACCGGACGCATTCCTCAGTCTTTTGGGAACTTGACTTCGCTTCAGGAGCTCCAATTGAGTGTCAATCAAATCTCAGGCGAGATCCCGGCGCAGCTAGGAAACTGCCGGCAACTCACTCATATCGAGCTGGATAACAACCAGATCACCGGAACGATTCCTTTCGAATTCGGCAGCTTATCCAATCTCACGATCCTCTTCTTGTGGCAAAACAAGCTTGAAGGAGCCATTCCTTCATCCATTTCCAACTGTGGCAATCTTGAAGCGGTTGATTTCTCACAAAACTTTTTGACTGGTCCCATTCCGGGTGGGATTTTCCAGCTCCAGAATCTCACCAAGCTTCTACTACTGTCGAACAATCTCTCCAGCGAAATCCCGCCGGAGATTGGAAACTGTTCCTCGTTGATTCGGTTTCGGGCGAACAACAACAAACTCACCGGAGCAATCCCGCAGCAGATTGGGAGTCTGAAGAGCTTGAATTTCCTCGATCTCGGATCGAATCGGCTCAACCGAAACATACCGGAGGGGATCGCCGGCTGCCGGAATCTGACGTTTCTCGACCTGCATTCCAATTCGATCACGGGAAACTTGCCTGCAGGTTTCAATCTGCTTGTGTCTCTCCAGTTCGTCGATTTCTCGGATAATATGATCGAAGGGACGTTGAGTCCTGGACTCGGTTCGCTGACGTCACTCACCAAGCTCGTTTTGGGGAACAACCGGTTCGCCGGTTCCATTCCGAGTCAACTCGGTTCGTGTGGGAAGTTACAGTTACTGGACCTTGGCGGGAACGAGCTAACCGGTGCAATCCCGGCGAGTTTGGGAAAGATTCCGGCGCTGGAAATCTCTCTGAATCTGAGCTGGAACCAACTCTCCGGCGAGTTACCGAAGGAGTTCGCCGATTTGGACAAGCTCGGCATTCTGGACCTCTCTTACAACCAGCTCTCCGGCGACCTCCAATTTCTCGCGGACATGCAAAATCTCGTAGTCCTTAACGTCTCGCACAACAACTTCACCGGGCGCGTGCCGGACACGCCCTTCTTCGCGAAGCTCCCTCTCAGCGTCATGTCCGGCAACCCCTCCTCTGCCTCACCGGCAGCCAGTGCGCCGACGCAGGAAACCCCGGCTGCGGCGCTCTACATCATCCTCGCAGCCCGAAAGCAGGCCCGGCCAGGGTTCTTCAGCGGGTCCCACGAGCCCGATCCGGAGGACGACACCGAAGTTGACGTGGGCCCACCGTGGGAGGTGACGCTCTACCAGAAGCTCGACCTCTCGATCGCCGACGTGGCGAAAAACCTGACGCCTGACAACGTCATAGGCCGGGGGCGATCCGGCATCGTTTACCACGTGTCAATCCCCTCGGGACTCTCCCTCGCCGTGAAAAGATTCCGTACCGCGGAGAAACACTCCGCCGCCGCTTTTTCCTCCGAGATTGCCACGCTGGCAAGGATACGTCACCGTAACATTGTCAGGCTACTGGGCTGGGGGGCCAACCGGAGAACGAAGTTACTGTTTTACGATTACTTGGCACAGGGTAATTTGGGCTCGTTGCTCCACGAGGGGTGCGCAGGATTAGTACAGTGGGACACGCGGTTCAAGATAGCACTGGGCGTGGCGGAGGGCTTGGCTTATTTGCACCACGATTGCCAGCCGGCTATTTTGCACCGGGATGTGAAGGCCCAGAACATTTTGCTCGGGGATCAATACGAGGCCGTTTTGGCCGACTTTGGGCTCGCTAGGTTGGTTGAAGACGATCCAAACGGTCCGTTTTCGGCCAACCCCCAGTTTGCTGGTTCATACGGCTACATTGCACCTGATGCAATCAAACCACTTGCAGAATATGCCTGCATGCTGAAAATTACCGCGAAAAGTGATGTGTATAGCTATGGGGTAGTCCTTTTGGAGATCATAACTGGGAAAAGGCCAGCAGACCCCTCATTCACCGACGGCCAGCATGTGATTCAGTGGGTGCGAGACCACTTGAAGAGCAAGAAGGATCCGATAGAAATTCTTGATCAGAAGCTCCAAGGCTACCCGGATACGCAGATACAAGAAATGCTCCAAGCGCTTGGAATTTCGCTCCTATGTACCAGCAACAGGGCGGAAGATCGTCCCACGATGAAGGATGTGGCTGCATTGTTGAGAGAAATTCGACATGATCAGCCCGCGACAGGAAGCGAGGCACACAAGCCTGCTAGCACCGCGTTGAAGAACTCATCTTCTTCCGAGGAGCGTGGAGTTGATGAGCGGCCGGGCGAGGTCGAGCTCGCAGACCTTGGCGCCGACGTGGCGGGCGAGGTCGCGGATCTTGGCGCCGACCCCGAGGAGGGCAAACACTACCGTACCTCCGCATCATCTAAGAAAGACTCGGTGTTGGGAGACAAGCTCGAGGGCGGTCATGGCGCAGAAGACGACGTCTGTCTCCGAGACGGAAATGAACTCGGTCAACCGACGCCGAATAGCCGATCGAAATCCGAGGCAACCGATTTCTCGTCTAATCGCCGCCGGGTCGGATCGAACCCGCCGCCCGTGATTTATGAATTGGAGGACCTCGAGCTCGGAGACATGGACGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1174

Amino Acids

127.08

Weight (kDa)

4.98

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 67 8.7e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 89 - 196 1.2e-10 Leucine-rich repeat region
LRR_8 PF13855 142 - 201 8.2e-07 Leucine rich repeat
LRR_14 PF23598 161 - 263 3.2e-09 Leucine-rich repeat region
LRR_14 PF23598 253 - 361 1.7e-08 Leucine-rich repeat region
LRR_8 PF13855 283 - 342 2.8e-07 Leucine rich repeat
LRR_8 PF13855 308 - 366 9.3e-07 Leucine rich repeat
LRR_8 PF13855 458 - 510 6.7e-06 Leucine rich repeat
LRR_14 PF23598 464 - 656 1.5e-10 Leucine-rich repeat region
LRR_8 PF13855 547 - 607 2.8e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 752 - 1028 1.2e-34 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 753 - 1026 2.2e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013666)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05780 FvH4_5g05780 FvH4_5g05780
malus_domestica MD06G1170200.v1.1
prunus_persica Prupe.5G169700_v2.0.a1
pyrus_communis pycom06g15160
rosa_chinensis RchiOBHm_Chr7g0193041
rosa_laevigata RLG00000004301
rosa_multiflora Rmu_co8431803.1_g000001 Rmu_co8478551.1_g000001 Rmu_sc0002040.1_g000078
rosa_roxburghii Rroxscaffold_3G00262440
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132300 Rh7BG133200 Rh7CG136300 Rh7DG134200
rosa_wichuraiana Rw7G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 203
AatII GACGTC 2 cut(s) 1640, 3366
Acc16I TGCGCA 1 cut(s) 2515
Acc36I ACCTGC 3 cut(s) 203, 1521, 1538
AccB1I GGYRCC 3 cut(s) 256, 3227, 3260
AccB7I CCANNNNNTGG 3 cut(s) 217, 704, 925
AccBSI CCGCTC 2 cut(s) 393, 3196
AccII CGCG 9 cut(s) 1916, 1971, 1994, 2345, 2539, 2813, 3116, 3152, 3251
AccIII TCCGGA 1 cut(s) 2152
AcoI YGGCCR 5 cut(s) 2117, 2667, 2721, 2896, 3197
AcsI RAATTY 9 cut(s) 226, 265, 543, 946, 1118, 1429, 2955, 3021, 3096
AcuI CTGAAG 5 cut(s) 524, 836, 980, 1439, 2113
AcvI CACGTG 1 cut(s) 2296
AcyI GRCGYC 6 cut(s) 1637, 2249, 3228, 3261, 3363, 3401
AfaI GTAC 4 cut(s) 2341, 2526, 3037, 3292
AfeI AGCGCT 1 cut(s) 3015
AflIII ACRYGT 1 cut(s) 2295
AgeI ACCGGT 2 cut(s) 1668, 1748
AhdI GACNNNNNGTC 1 cut(s) 1614
AjiI CACGTC 3 cut(s) 2176, 2233, 3235
AjnI CCWGG 3 cut(s) 93, 1618, 2119
AloI GAACNNNNNNTCC 6 cut(s) 1298, 1330, 1805, 1837, 2525, 2557
Alw21I GWGCWC 6 cut(s) 762, 780, 906, 1005, 3216, 3507
AlwNI CAGNNNCTG 1 cut(s) 1723
Ama87I CYCGRG 6 cut(s) 722, 2308, 2642, 3269, 3335, 3500
Aor13HI TCCGGA 1 cut(s) 2152
Aor51HI AGCGCT 1 cut(s) 3015
ApaI GGGCCC 1 cut(s) 2183
ApeKI GCWGC 9 cut(s) 421, 1045, 1125, 1402, 1488, 2045, 2075, 2099, 3080
ApoI RAATTY 9 cut(s) 226, 265, 543, 946, 1118, 1429, 2955, 3021, 3096
AsiGI ACCGGT 2 cut(s) 1668, 1748
Asp700I GAANNNNTTC 3 cut(s) 1345, 1841, 2333
AsuII TTCGAA 1 cut(s) 1116
AsuNHI GCTAGC 1 cut(s) 3143
AvaI CYCGRG 6 cut(s) 722, 2308, 2642, 3269, 3335, 3500
AxyI CCTNAGG 1 cut(s) 135
BaeGI GKGCMC 3 cut(s) 261, 624, 2183
BaeI ACNNNNGTAYC 2 cut(s) 3274, 3307
BamHI GGATCC 1 cut(s) 2944
BanI GGYRCC 3 cut(s) 256, 3227, 3260
BauI CACGAG 3 cut(s) 333, 2141, 2504
BbrPI CACGTG 1 cut(s) 2296
BbsI GAAGAC 2 cut(s) 2703, 3363
Bbv12I GWGCWC 6 cut(s) 762, 780, 906, 1005, 3216, 3507
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 9 cut(s) 408, 1057, 1137, 1414, 1475, 2057, 2062, 2111, 3067
BceAI ACGGC 4 cut(s) 2309, 2645, 2764, 2911
BciT130I CCWGG 3 cut(s) 95, 1620, 2121
BciVI GTATCC 2 cut(s) 2391, 2980
BclI TGATCA 2 cut(s) 2962, 3106
BfaI CTAG 5 cut(s) 248, 549, 1049, 2685, 3144
BfmI CTRYAG 1 cut(s) 1545
BfoI RGCGCY 5 cut(s) 1787, 2084, 3017, 3231, 3264
BfuAI ACCTGC 3 cut(s) 203, 1521, 1538
BfuI GTATCC 2 cut(s) 2391, 2980
BglI GCCNNNNNGGC 5 cut(s) 2042, 2234, 2390, 2666, 3236
BglII AGATCT 1 cut(s) 411
BlpI GCTNAGC 2 cut(s) 143, 533
BmeRI GACNNNNNGTC 1 cut(s) 1614
BmeT110I CYCGRG 6 cut(s) 722, 2308, 2642, 3269, 3335, 3500
BmgBI CACGTC 3 cut(s) 2176, 2233, 3235
BmrI ACTGGG 6 cut(s) 398, 470, 2435, 2564, 2725, 2871
BmsI GCATC 3 cut(s) 2292, 2755, 3308
BmtI GCTAGC 1 cut(s) 3147
BmuI ACTGGG 6 cut(s) 398, 470, 2435, 2564, 2725, 2871
BoxI GACNNNNGTC 1 cut(s) 3366
BpiI GAAGAC 2 cut(s) 2703, 3363
BplI GAGNNNNNCTC 4 cut(s) 2339, 2371, 3318, 3350
BpmI CTGGAG 5 cut(s) 482, 612, 1259, 1300, 1556
Bpu10I CCTNAGC 4 cut(s) 374, 389, 426, 446
Bpu1102I GCTNAGC 2 cut(s) 143, 533
Bpu14I TTCGAA 1 cut(s) 1116
BsaAI YACGTR 1 cut(s) 2296
BsaBI GATNNNNATC 1 cut(s) 3418
BsaHI GRCGYC 6 cut(s) 1637, 2249, 3228, 3261, 3363, 3401
BsaI GGTCTC 1 cut(s) 2916
BsaWI WCCGGW 9 cut(s) 898, 956, 1100, 1388, 1471, 1668, 1748, 2152, 2442
Bse118I RCCGGY 9 cut(s) 320, 456, 1059, 1484, 1668, 1676, 1748, 2041, 2599
Bse21I CCTNAGG 1 cut(s) 135
Bse3DI GCAATG 1 cut(s) 2754
Bse8I GATNNNNATC 1 cut(s) 3418
BseAI TCCGGA 1 cut(s) 2152
BseBI CCWGG 3 cut(s) 95, 1620, 2121
BseGI GGATG 4 cut(s) 1187, 2091, 2623, 3079
BseJI GATNNNNATC 1 cut(s) 3418
BseMI GCAATG 1 cut(s) 2754
BseRI GAGGAG 4 cut(s) 701, 2020, 3191, 3287
BseSI GKGCMC 3 cut(s) 261, 624, 2183
BseX3I CGGCCG 1 cut(s) 3197
BseXI GCAGC 9 cut(s) 408, 1057, 1137, 1414, 1475, 2057, 2062, 2111, 3067
BseYI CCCAGC 1 cut(s) 2430
BsgI GTGCAG 2 cut(s) 625, 754
Bsh1236I CGCG 9 cut(s) 1916, 1971, 1994, 2345, 2539, 2813, 3116, 3152, 3251
Bsh1285I CGRYCG 3 cut(s) 2226, 3200, 3416
BshNI GGYRCC 3 cut(s) 256, 3227, 3260
BshTI ACCGGT 2 cut(s) 1668, 1748
BsiEI CGRYCG 3 cut(s) 2226, 3200, 3416
BsiHKAI GWGCWC 6 cut(s) 762, 780, 906, 1005, 3216, 3507
BsiHKCI CYCGRG 6 cut(s) 722, 2308, 2642, 3269, 3335, 3500
BslFI GGGAC 8 cut(s) 167, 797, 1233, 1621, 2122, 2325, 2546, 3045
BsmBI CGTCTC 3 cut(s) 757, 1951, 3369
BsmFI GGGAC 8 cut(s) 167, 797, 1233, 1621, 2122, 2325, 2546, 3045
BsmI GAATGC 3 cut(s) 963, 1516, 1866
Bso31I GGTCTC 1 cut(s) 2916
BsoBI CYCGRG 6 cut(s) 722, 2308, 2642, 3269, 3335, 3500
Bsp119I TTCGAA 1 cut(s) 1116
Bsp120I GGGCCC 1 cut(s) 2179
Bsp13I TCCGGA 1 cut(s) 2152
Bsp1720I GCTNAGC 2 cut(s) 143, 533
Bsp19I CCATGG 1 cut(s) 14
Bsp68I TCGCGA 1 cut(s) 1994
BspEI TCCGGA 1 cut(s) 2152
BspFNI CGCG 9 cut(s) 1916, 1971, 1994, 2345, 2539, 2813, 3116, 3152, 3251
BspMAI CTGCAG 1 cut(s) 1549
BspMI ACCTGC 3 cut(s) 203, 1521, 1538
BspOI GCTAGC 1 cut(s) 3147
BspQI GCTCTTC 2 cut(s) 1415, 2927
BspT104I TTCGAA 1 cut(s) 1116
BspT107I GGYRCC 3 cut(s) 256, 3227, 3260
BspTNI GGTCTC 1 cut(s) 2916
BsrBI CCGCTC 2 cut(s) 393, 3196
BsrDI GCAATG 1 cut(s) 2754
BsrFI RCCGGY 9 cut(s) 320, 456, 1059, 1484, 1668, 1676, 1748, 2041, 2599
BssAI RCCGGY 9 cut(s) 320, 456, 1059, 1484, 1668, 1676, 1748, 2041, 2599
BssNI GRCGYC 6 cut(s) 1637, 2249, 3228, 3261, 3363, 3401
BssSI CACGAG 3 cut(s) 333, 2141, 2504
BssT1I CCWWGG 5 cut(s) 14, 703, 1729, 2974, 3223
Bst2BI CACGAG 3 cut(s) 333, 2141, 2504
Bst2UI CCWGG 3 cut(s) 95, 1620, 2121
Bst6I CTCTTC 5 cut(s) 32, 49, 1154, 1415, 2927
BstACI GRCGYC 6 cut(s) 1637, 2249, 3228, 3261, 3363, 3401
BstAPI GCANNNNNTGC 3 cut(s) 2051, 2765, 2789
BstBAI YACGTR 1 cut(s) 2296
BstBI TTCGAA 1 cut(s) 1116
BstDSI CCRYGG 3 cut(s) 14, 2186, 2343
BstEII GGTNACC 1 cut(s) 190
BstF5I GGATG 4 cut(s) 1187, 2091, 2623, 3079
BstFNI CGCG 9 cut(s) 1916, 1971, 1994, 2345, 2539, 2813, 3116, 3152, 3251
BstH2I RGCGCY 5 cut(s) 1787, 2084, 3017, 3231, 3264
BstMCI CGRYCG 3 cut(s) 2226, 3200, 3416
BstNI CCWGG 3 cut(s) 95, 1620, 2121
BstNSI RCATGY 3 cut(s) 1924, 2800, 2906
BstPAI GACNNNNGTC 1 cut(s) 3366
BstPI GGTNACC 1 cut(s) 190
BstSFI CTRYAG 1 cut(s) 1545
BstSLI GKGCMC 3 cut(s) 261, 624, 2183
BstUI CGCG 9 cut(s) 1916, 1971, 1994, 2345, 2539, 2813, 3116, 3152, 3251
BstV1I GCAGC 9 cut(s) 408, 1057, 1137, 1414, 1475, 2057, 2062, 2111, 3067
BstV2I GAAGAC 2 cut(s) 2703, 3363
BstX2I RGATCY 4 cut(s) 411, 1035, 2944, 3253
BstXI CCANNNNNNTGG 4 cut(s) 180, 632, 2738, 3017
BstYI RGATCY 4 cut(s) 411, 1035, 2944, 3253
BstZI CGGCCG 1 cut(s) 3197
Bsu36I CCTNAGG 1 cut(s) 135
BsuI GTATCC 2 cut(s) 2391, 2980
BtgI CCRYGG 3 cut(s) 14, 2186, 2343
BtrI CACGTC 3 cut(s) 2176, 2233, 3235
BtsCI GGATG 4 cut(s) 1187, 2091, 2623, 3079
BtsIMutI CAGTG 5 cut(s) 365, 2056, 2534, 2552, 2918
BtuMI TCGCGA 1 cut(s) 1994
BveI ACCTGC 3 cut(s) 203, 1521, 1538
CaiI CAGNNNCTG 1 cut(s) 1723
Cfr10I RCCGGY 9 cut(s) 320, 456, 1059, 1484, 1668, 1676, 1748, 2041, 2599
Cfr42I CCGCGG 1 cut(s) 2346
CpoI CGGWCCG 1 cut(s) 2711
CseI GACGC 6 cut(s) 969, 1999, 2068, 2206, 2257, 3409
Csp6I GTAC 4 cut(s) 2340, 2525, 3036, 3291
CspAI ACCGGT 2 cut(s) 1668, 1748
CspI CGGWCCG 1 cut(s) 2711
CviQI GTAC 4 cut(s) 2340, 2525, 3036, 3291
DinI GGCGCC 2 cut(s) 3229, 3262
DriI GACNNNNNGTC 1 cut(s) 1614
EaeI YGGCCR 5 cut(s) 2117, 2667, 2721, 2896, 3197
EagI CGGCCG 1 cut(s) 3197
Eam1104I CTCTTC 5 cut(s) 32, 49, 1154, 1415, 2927
Eam1105I GACNNNNNGTC 1 cut(s) 1614
EarI CTCTTC 5 cut(s) 32, 49, 1154, 1415, 2927
EciI GGCGGA 3 cut(s) 2347, 2579, 3065
Ecl136II GAGCTC 6 cut(s) 760, 778, 904, 1003, 3214, 3505
EclXI CGGCCG 1 cut(s) 3197
Eco130I CCWWGG 5 cut(s) 14, 703, 1729, 2974, 3223
Eco31I GGTCTC 1 cut(s) 2916
Eco47III AGCGCT 1 cut(s) 3015
Eco52I CGGCCG 1 cut(s) 3197
Eco53kI GAGCTC 6 cut(s) 760, 778, 904, 1003, 3214, 3505
Eco57I CTGAAG 5 cut(s) 524, 836, 980, 1439, 2113
Eco72I CACGTG 1 cut(s) 2296
Eco81I CCTNAGG 1 cut(s) 135
Eco88I CYCGRG 6 cut(s) 722, 2308, 2642, 3269, 3335, 3500
Eco91I GGTNACC 1 cut(s) 190
EcoICRI GAGCTC 6 cut(s) 760, 778, 904, 1003, 3214, 3505
EcoO109I RGGNCCY 4 cut(s) 154, 614, 2136, 3496
EcoO65I GGTNACC 1 cut(s) 190
EcoRI GAATTC 2 cut(s) 946, 1118
EcoRII CCWGG 3 cut(s) 93, 1618, 2119
EcoT14I CCWWGG 5 cut(s) 14, 703, 1729, 2974, 3223
EgeI GGCGCC 2 cut(s) 3229, 3262
EheI GGCGCC 2 cut(s) 3229, 3262
ErhI CCWWGG 5 cut(s) 14, 703, 1729, 2974, 3223
Esp3I CGTCTC 3 cut(s) 757, 1951, 3369
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FaqI GGGAC 8 cut(s) 167, 797, 1233, 1621, 2122, 2325, 2546, 3045
FauI CCCGC 9 cut(s) 170, 530, 1335, 1407, 1727, 2126, 3121, 3232, 3477
FbaI TGATCA 2 cut(s) 2962, 3106
FokI GGATG 4 cut(s) 1174, 2078, 2630, 3086
FspBI CTAG 5 cut(s) 248, 549, 1049, 2685, 3144
FspI TGCGCA 1 cut(s) 2515
GsaI CCCAGC 1 cut(s) 2434
GsuI CTGGAG 5 cut(s) 482, 612, 1259, 1300, 1556
HaeII RGCGCY 5 cut(s) 1787, 2084, 3017, 3231, 3264
HgaI GACGC 6 cut(s) 969, 1999, 2068, 2206, 2257, 3409
Hin1I GRCGYC 6 cut(s) 1637, 2249, 3228, 3261, 3363, 3401
HincII GTYRAC 3 cut(s) 1694, 2173, 3395
HindII GTYRAC 3 cut(s) 1694, 2173, 3395
HindIII AAGCTT 2 cut(s) 1166, 1289
Hpy166II GTNNAC 5 cut(s) 61, 1694, 2173, 2290, 3395
Hpy8I GTNNAC 5 cut(s) 61, 1694, 2173, 2290, 3395
Hpy99I CGWCG 7 cut(s) 1583, 2062, 2234, 2897, 3236, 3365, 3403
Hsp92I GRCGYC 6 cut(s) 1637, 2249, 3228, 3261, 3363, 3401
KasI GGCGCC 2 cut(s) 3227, 3260
KflI GGGWCCC 2 cut(s) 154, 2136
Kpn2I TCCGGA 1 cut(s) 2152
KroI GCCGGC 3 cut(s) 1059, 1484, 2599
KroNI GCCGGC 3 cut(s) 1061, 1486, 2601
Ksp22I TGATCA 2 cut(s) 2962, 3106
KspI CCGCGG 1 cut(s) 2346
LguI GCTCTTC 2 cut(s) 1415, 2927
Lsp1109I GCAGC 9 cut(s) 408, 1057, 1137, 1414, 1475, 2057, 2062, 2111, 3067
LweI GCATC 3 cut(s) 2292, 2755, 3308
MaeI CTAG 5 cut(s) 248, 549, 1049, 2685, 3144
MbiI CCGCTC 2 cut(s) 393, 3196
MfeI CAATTG 3 cut(s) 148, 910, 1007
MflI RGATCY 4 cut(s) 411, 1035, 2944, 3253
Mly113I GGCGCC 2 cut(s) 3228, 3261
MlyI GAGTC 7 cut(s) 127, 1423, 1617, 1624, 1699, 2307, 3308
MmeI TCCRAC 2 cut(s) 1221, 3439
MroI TCCGGA 1 cut(s) 2152
MroNI GCCGGC 3 cut(s) 1059, 1484, 2599
MroXI GAANNNNTTC 3 cut(s) 1345, 1841, 2333
MseI TTAA 2 cut(s) 35, 1941
MslI CAYNNNNRTG 3 cut(s) 178, 941, 2616
MspA1I CMGCKG 3 cut(s) 537, 2133, 2345
MunI CAATTG 3 cut(s) 148, 910, 1007
Mva1269I GAATGC 3 cut(s) 963, 1516, 1866
MvaI CCWGG 3 cut(s) 95, 1620, 2121
MvnI CGCG 9 cut(s) 1916, 1971, 1994, 2345, 2539, 2813, 3116, 3152, 3251
NaeI GCCGGC 3 cut(s) 1061, 1486, 2601
NarI GGCGCC 2 cut(s) 3228, 3261
NcoI CCATGG 1 cut(s) 14
NgoMIV GCCGGC 3 cut(s) 1059, 1484, 2599
NheI GCTAGC 1 cut(s) 3143
NmeAIII GCCGAG 2 cut(s) 885, 1842
NmuCI GTSAC 5 cut(s) 190, 914, 1638, 2194, 2402
NruI TCGCGA 1 cut(s) 1994
NsbI TGCGCA 1 cut(s) 2515
NspI RCATGY 3 cut(s) 1924, 2800, 2906
NspV TTCGAA 1 cut(s) 1116
PaeI GCATGC 1 cut(s) 2800
PaeR7I CTCGAG 3 cut(s) 722, 3335, 3500
PaqCI CACCTGC 1 cut(s) 203
PciSI GCTCTTC 2 cut(s) 1415, 2927
PcsI WCGNNNNNNNCGW 3 cut(s) 1459, 2292, 3240
PctI GAATGC 3 cut(s) 963, 1516, 1866
PdiI GCCGGC 3 cut(s) 1061, 1486, 2601
PdmI GAANNNNTTC 3 cut(s) 1345, 1841, 2333
PflFI GACNNNGTC 2 cut(s) 2258, 3362
PflMI CCANNNNNTGG 3 cut(s) 217, 704, 925
PfoI TCCNGGA 2 cut(s) 780, 1618
PinAI ACCGGT 2 cut(s) 1668, 1748
Ple19I CGATCG 2 cut(s) 2226, 3416
PleI GAGTC 7 cut(s) 126, 1422, 1617, 1623, 1698, 2307, 3308
PluTI GGCGCC 2 cut(s) 3231, 3264
PmaCI CACGTG 1 cut(s) 2296
PmlI CACGTG 1 cut(s) 2296
PpsI GAGTC 7 cut(s) 126, 1422, 1617, 1623, 1698, 2307, 3308
Ppu21I YACGTR 1 cut(s) 2296
PpuMI RGGWCCY 4 cut(s) 154, 614, 2136, 3496
PshAI GACNNNNGTC 1 cut(s) 3366
Psp124BI GAGCTC 6 cut(s) 762, 780, 906, 1005, 3216, 3507
Psp5II RGGWCCY 4 cut(s) 154, 614, 2136, 3496
Psp6I CCWGG 3 cut(s) 93, 1618, 2119
PspCI CACGTG 1 cut(s) 2296
PspEI GGTNACC 1 cut(s) 190
PspFI CCCAGC 1 cut(s) 2430
PspGI CCWGG 3 cut(s) 93, 1618, 2119
PspOMI GGGCCC 1 cut(s) 2179
PspPPI RGGWCCY 4 cut(s) 154, 614, 2136, 3496
PspXI VCTCGAGB 2 cut(s) 3335, 3500
PstI CTGCAG 1 cut(s) 1549
PstNI CAGNNNCTG 1 cut(s) 1723
PsuI RGATCY 4 cut(s) 411, 1035, 2944, 3253
PsyI GACNNNGTC 2 cut(s) 2258, 3362
PvuI CGATCG 2 cut(s) 2226, 3416
RruI TCGCGA 1 cut(s) 1994
RsaI GTAC 4 cut(s) 2341, 2526, 3037, 3292
RsaNI GTAC 4 cut(s) 2340, 2525, 3036, 3291
RseI CAYNNNNRTG 3 cut(s) 178, 941, 2616
Rsr2I CGGWCCG 1 cut(s) 2711
RsrII CGGWCCG 1 cut(s) 2711
SacI GAGCTC 6 cut(s) 762, 780, 906, 1005, 3216, 3507
SacII CCGCGG 1 cut(s) 2346
SapI GCTCTTC 2 cut(s) 1415, 2927
SaqAI TTAA 2 cut(s) 35, 1941
SbfI CCTGCAGG 1 cut(s) 1549
SchI GAGTC 7 cut(s) 127, 1423, 1617, 1624, 1699, 2307, 3308
SdaI CCTGCAGG 1 cut(s) 1549
SfaNI GCATC 3 cut(s) 2292, 2755, 3308
SfcI CTRYAG 1 cut(s) 1545
SfiI GGCCNNNNNGGCC 1 cut(s) 2666
SfoI GGCGCC 2 cut(s) 3229, 3262
Sfr274I CTCGAG 3 cut(s) 722, 3335, 3500
Sfr303I CCGCGG 1 cut(s) 2346
SfuI TTCGAA 1 cut(s) 1116
SgrBI CCGCGG 1 cut(s) 2346
SlaI CTCGAG 3 cut(s) 722, 3335, 3500
SmiMI CAYNNNNRTG 3 cut(s) 178, 941, 2616
SmlI CTYRAG 3 cut(s) 722, 3335, 3500
SmoI CTYRAG 3 cut(s) 722, 3335, 3500
SphI GCATGC 1 cut(s) 2800
Sse8387I CCTGCAGG 1 cut(s) 1549
SspDI GGCGCC 2 cut(s) 3227, 3260
SspMI CTAG 5 cut(s) 248, 549, 1049, 2685, 3144
SstI GAGCTC 6 cut(s) 762, 780, 906, 1005, 3216, 3507
StyI CCWWGG 5 cut(s) 14, 703, 1729, 2974, 3223
TaqII GACCGA 1 cut(s) 3380
TatI WGTACW 1 cut(s) 2524
TauI GCSGC 9 cut(s) 396, 571, 816, 2081, 2363, 2366, 3199, 3453, 3475
Tru1I TTAA 2 cut(s) 35, 1941
Tru9I TTAA 2 cut(s) 35, 1941
TscAI CASTG 5 cut(s) 372, 2056, 2534, 2559, 2918
TseFI GTSAC 5 cut(s) 190, 914, 1638, 2194, 2402
TseI GCWGC 9 cut(s) 421, 1045, 1125, 1402, 1488, 2045, 2075, 2099, 3080
Tsp45I GTSAC 5 cut(s) 190, 914, 1638, 2194, 2402
TspDTI ATGAA 8 cut(s) 96, 180, 959, 1176, 2733, 3083, 3399, 3501
TspGWI ACGGA 3 cut(s) 2327, 2703, 3393
TspRI CASTG 5 cut(s) 372, 2056, 2534, 2559, 2918
Tth111I GACNNNGTC 2 cut(s) 2258, 3362
Van91I CCANNNNNTGG 3 cut(s) 217, 704, 925
XapI RAATTY 9 cut(s) 226, 265, 543, 946, 1118, 1429, 2955, 3021, 3096
XceI RCATGY 3 cut(s) 1924, 2800, 2906
XcmI CCANNNNNNNNNTGG 1 cut(s) 1654
XhoI CTCGAG 3 cut(s) 722, 3335, 3500
XmnI GAANNNNTTC 3 cut(s) 1345, 1841, 2333
XspI CTAG 5 cut(s) 248, 549, 1049, 2685, 3144
ZraI GACGTC 2 cut(s) 1638, 3364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.