Rh7CG136300

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0013666
Physical Location & Seq
Forward (+)
11089241 .. 11094154
4914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG136300.1

Sequence Viewer

Length: 3336 bp
ATGCCTGTAAATCCATGGACCTTCCTCTTCTTCTTAACCCTCTCTTCTATTCTCCTGGTTCACTTTCCTGCTCTGGCCTTGAACCAACAAGGCCAGGCTCTTGTTTCATGGAAGCAGAGTCTCAATGGCTCCCCTGAGGGGCTGAGCAATTGGGACCCAAGTGATGAAACTCCATGCGGGTGGTCCGGGGTCACCTGCAACTTCAACAACCAAGTTGCGGAGCTGAATTTGAAGTTTATTGATCTTCTAGGTAAGGTGCCCTGGAATTTCACTTCGTTGCTGACCCTAAACAAGCTCATCTTGTCCGGCACAAACCTCACCGGCTCAATCCCACGAGAAATCTCTACTCTTAAACAACTGACCTCACTGGACCTGAGCGACAATGCACTGAGCGGCGAAATCCCAGTTGAGATCTGCGAGCTGCCTAAGCTCGAAGAACTCTACCTCAGCACGAACCGGCTGGAGGGCTCAATCCCAGTCCAAATCGGAAACCTCACAAACCTGAAGTGGCTGGTTCTCTACGACAATCAGCTCAGCGGGAAAATTCCTAGCACCATTGGAAATCTGCCTCAACTTCAAGTAATCAGAGCTGGAGGGAACAAGAACCTTGAAGGTCCTGTGCCTCATGAGATTGGGAACTGCACCGATTTGGTCATGTTGGGCTTAGCAGAAACCAGCATTTCCGGCTTCCTCCCTCCAAGCCTTGGCCTCCTCAAAAAACTCGAGACATTGGCAATCTACACCACCCTCGTCTCCGGTCCGATCCCCCAAGAGCTCGGAGACTGCACCGAGCTCCGGGACATCTACTTGTACGAAAACTCCATTAGCGGCTCTGTTCCGAGCAAATTAGGCAACTTGAAAATTCTTCAAAACCTTCTGCTATGGCAAAACAGCCTTGTAGGGGTTCTTCCACCGGAGCTCGGCAATTGTCACCAGCTATTGGTCATTGACATTTCTATGAATTCCTTGACCGGAAGCATTCCTCAGTCTTTTGGGAACTTGACCTCGCTTCAGGAGCTCCAATTGAGTGTCAATCAAATCTCAGGCGAGATCCCGGCGCAGCTAGGAAACTGCCGGCAACTCACTCATATCGAGCTGGATAACAACCAGATCACAGGAACGATTCCTTTCGAATTCGGCCGCTTATCCAATCTCACGATCCTCTTCTTGTGGCAAAACAAGCTTGAAGGAGCCATTCCTTCATCGATTTCCAACTGTGGCAATCTTGAAGCGGTTGATTTCTCACAAAACTTTTTGACCGGTCCCATTCCGGGTGGGATTTTCCAGCTCCAGAATCTCACCAAGCTTCTACTACTGTCGAACAATCTCTCCAGCGAAATCCCGCCCGAGATCGGAAACTGCTCCTCGTTGATTCGGTTTCGGGCGAACAACAACAAGCTCACCGGAGCAATCCCGCAGCAGATTGGGAGTTTGAAGAGCTTGAATTTCCTCGATCTCGGATCGAATCGGCTCAACCGAAACATACCGGACGGGATCGCCGGCTGCCGGAATCTGACTTTTCTCGACCTGCATTCCAATTCGATCACCGGAAACTTGCCTGCAGGCTTCAATCTGCTTGTGTCTCTCCAGTTCGTCGATTTCTCGGATAATATGATCGAAGGGACGTTGAGTCCTGGACTCGGTTCGCTGACGTCACTCACCAAGCTCGTTTTGGGGAACAACCGGTTCGCCGGTTCCATTCCGAGTCAACTCGGTTCGTGTGGGAAGTTACAGTTACTGGACCTTGGCGGGAATGAGCTAACAGGTACAATCCCGGCGAGTTTGGGAAAGATTCCGGCACTGGAAATCTCTCTGAACCTGAGCTGGAACCAACTCTCCGGCGAGTTACCGAAGGAGTTCGCCGATTTGGACAAGCTCGGCATTCTGGACCTCTCTCACAACCAGCTTTCCGGAGACCTCCAGTTTCTTGCGGACATGCAAAATCTCGTCGTGCTTAACGTCTCGCACAACAACTTCACCGGGCGCGTGCCGGACACGCCCTTCTTCGCGAAGCTCCCTCTCAGCGTCATGTCCGGCAACCCCTCCCTCTGCCTCACCGGCAGCCAGTGCGCTGATGCAGGAAAACCCGGCTGGTCCCGCCGCCGCAACGCCGCCGCGCGCGTGGCGATGGTCGTCCTCCTCTGCACCGCCTGCACACTCCTCTTAGCGGCGCTCTACATCATCCTCGCAGCCCGAAAGCGGGCCCGGCCAGGGTTCTTCAGCGGGGCCCACGAGCCCGATCCGGAGGACGACACCGAAGTCGACGTGGGCCCACCGTGGGAGGTGACGCTCTACCAGAAACTCGACCTGTCGATCGCCGACGTGGCGAAAAGGCTGACGCCTGACAACGTCATAGGCCGGGGGCGATCCGGCGTCGTTTACCACGTGTCAATCCCCTCGGGACTCTCCCTCGCCGTGAAAAGATTCCGTAACGCGGAGAAACACTCCGCCGCCGCTTTTTCCTCCGAGATTGCCACGCTGGCAAGGATACGTCACCGTAACATTGTCAGGCTACTGGGCTGGGGGGCCAACCGGAGAACGAAGTTACTGTTTTACGATTACTTGGCACAGGGTAATTTGGGCTCGTTGCTCCACGAGGGGTGCGCAGGATTAGTAGAGTGGGACACGCGGTTCAAGATAGCACTCGGCGTGGCGGAGGGTTTGGCTTATTTGCACCACGATTGCCAGCCGGCTATTTTGCACCGGGATGTGAAGGCCCAGAATATTTTGCTCGGGGATCAATACGAGGCCGTTTTGGCAGACTTTGGGCTCGCTAGGTTGGTTGAAGAAGATCAAAACGGTCCGTTTTCGGCCGACCCCCAGTTTGCTGGTTCTTACGGCTACATTGCGCCTGAATATGCCTGTATGCTGAAAATTACTGCGAAAACTGATGTGTATAGCTATGGGGTAGTCCTTTTGGAGATCATAACTGGGAAAAGGCCAGCAGACCCCTCATTCACCGACGGCCAGCATGTGATTCAGTGGGTGCGAGACCACTTGAAGAGCAAGAAGGATCCGATAGAGATTCTTGATCAGAAGCTCCAAGGCTACCCGGACACGCAGATACAAGAAATGCTCCAAGCGCTTGGAATTTCGCTCCTATGTACCAGCAACAGGGCGGAAGATCGTCCCACGATGAAGGATGTGGCTGCATTGTTGAGAGAAATTCGACATGATCAGCCCGCGACAGGAAGCGAGGCACACAAGCCTGCTAGCACCGCGTTGAAGAACTCATCTTCTTCAGTGACTCCAGCTCAACTGCTGATGATGCAAGGATCTTCTCATTGCTCACTTGCCTACTCTTCTTCAGCTGGTTATCTATCAGGAACCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1111

Amino Acids

120.4

Weight (kDa)

5.64

Isoelectric Point (pI)

35.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 67 8.2e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 91 - 196 6.7e-11 Leucine-rich repeat region
LRR_8 PF13855 96 - 154 2.2e-07 Leucine rich repeat
LRR_8 PF13855 142 - 201 8e-07 Leucine rich repeat
LRR_14 PF23598 161 - 269 1.6e-09 Leucine-rich repeat region
LRR_14 PF23598 258 - 366 2e-09 Leucine-rich repeat region
LRR_8 PF13855 289 - 347 6.1e-07 Leucine rich repeat
LRR_8 PF13855 313 - 371 8e-07 Leucine rich repeat
LRR_8 PF13855 463 - 515 4.2e-06 Leucine rich repeat
LRR_14 PF23598 469 - 661 3.4e-10 Leucine-rich repeat region
LRR_8 PF13855 552 - 612 2.6e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 782 - 1051 2.6e-37 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 783 - 1049 1.2e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013666)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05780 FvH4_5g05780 FvH4_5g05780
malus_domestica MD06G1170200.v1.1
prunus_persica Prupe.5G169700_v2.0.a1
pyrus_communis pycom06g15160
rosa_chinensis RchiOBHm_Chr7g0193041
rosa_laevigata RLG00000004301
rosa_multiflora Rmu_co8431803.1_g000001 Rmu_co8478551.1_g000001 Rmu_sc0002040.1_g000078
rosa_roxburghii Rroxscaffold_3G00262440
rosa_rugosa Rorug07G0007300
rosa_samantha Rh7AG132300 Rh7BG133200 Rh7CG136300 Rh7DG134200
rosa_wichuraiana Rw7G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 203
AasI GACNNNNNNGTC 1 cut(s) 2258
AatII GACGTC 1 cut(s) 1655
Acc16I TGCGCA 1 cut(s) 2605
Acc36I ACCTGC 2 cut(s) 203, 1536
AccB1I GGYRCC 1 cut(s) 256
AccB7I CCANNNNNTGG 2 cut(s) 704, 940
AccBSI CCGCTC 1 cut(s) 393
AccI GTMKAC 1 cut(s) 2262
AccII CGCG 9 cut(s) 1986, 2009, 2117, 2119, 2121, 2435, 2629, 3185, 3221
AccIII TCCGGA 2 cut(s) 1910, 2242
AcoI YGGCCR 4 cut(s) 1138, 2207, 2811, 2965
AcsI RAATTY 9 cut(s) 226, 265, 543, 861, 961, 1133, 1444, 3090, 3165
AcuI CTGAAG 5 cut(s) 524, 995, 2203, 3225, 3291
AcvI CACGTG 1 cut(s) 2386
AcyI GRCGYC 3 cut(s) 1652, 2339, 2373
AfaI GTAC 3 cut(s) 812, 1768, 3106
AfeI AGCGCT 1 cut(s) 3084
AflIII ACRYGT 1 cut(s) 2385
AgeI ACCGGT 2 cut(s) 1259, 1683
AhdI GACNNNNNGTC 1 cut(s) 1629
AjiI CACGTC 2 cut(s) 2266, 2323
AjnI CCWGG 5 cut(s) 54, 93, 260, 1633, 2209
AloI GAACNNNNNNTCC 6 cut(s) 1313, 1345, 1820, 1852, 2615, 2647
Alw21I GWGCWC 4 cut(s) 777, 795, 921, 1020
Alw26I GTCTC 8 cut(s) 125, 719, 757, 774, 1587, 1908, 1966, 2985
AlwNI CAGNNNCTG 1 cut(s) 1738
Ama87I CYCGRG 4 cut(s) 722, 1346, 2398, 2732
Aor13HI TCCGGA 2 cut(s) 1910, 2242
Aor51HI AGCGCT 1 cut(s) 3084
ApaI GGGCCC 3 cut(s) 2206, 2230, 2273
ApeKI GCWGC 7 cut(s) 421, 1060, 1417, 1503, 2061, 2189, 3149
ApoI RAATTY 9 cut(s) 226, 265, 543, 861, 961, 1133, 1444, 3090, 3165
ArsI GACNNNNNNTTYG 2 cut(s) 2807, 2839
AsiGI ACCGGT 2 cut(s) 1259, 1683
Asp700I GAANNNNTTC 2 cut(s) 1856, 2423
AspLEI GCGC 9 cut(s) 1060, 1986, 2072, 2119, 2121, 2173, 2606, 2851, 3085
AsuII TTCGAA 1 cut(s) 1131
AsuNHI GCTAGC 1 cut(s) 3212
AvaI CYCGRG 4 cut(s) 722, 1346, 2398, 2732
AxyI CCTNAGG 1 cut(s) 135
BaeGI GKGCMC 4 cut(s) 261, 2206, 2230, 2273
BamHI GGATCC 1 cut(s) 3013
BanI GGYRCC 1 cut(s) 256
BarI GAAGNNNNNNTAC 2 cut(s) 891, 923
BauI CACGAG 3 cut(s) 333, 2231, 2594
BbrPI CACGTG 1 cut(s) 2386
Bbv12I GWGCWC 4 cut(s) 777, 795, 921, 1020
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 7 cut(s) 408, 1072, 1429, 1490, 2073, 2201, 3136
BccI CCATC 1 cut(s) 2122
BceAI ACGGC 4 cut(s) 2399, 2735, 2854, 2980
BciT130I CCWGG 5 cut(s) 56, 95, 262, 1635, 2211
BciVI GTATCC 1 cut(s) 2481
BclI TGATCA 2 cut(s) 3031, 3175
BcoDI GTCTC 8 cut(s) 125, 719, 757, 774, 1587, 1908, 1966, 2985
BfaI CTAG 5 cut(s) 248, 549, 1064, 2775, 3213
BfmI CTRYAG 1 cut(s) 1560
BfoI RGCGCY 2 cut(s) 2174, 3086
BfuAI ACCTGC 2 cut(s) 203, 1536
BfuI GTATCC 1 cut(s) 2481
BglI GCCNNNNNGGC 4 cut(s) 2058, 2324, 2480, 2756
BglII AGATCT 1 cut(s) 411
BlpI GCTNAGC 3 cut(s) 143, 533, 664
BmeRI GACNNNNNGTC 1 cut(s) 1629
BmeT110I CYCGRG 4 cut(s) 722, 1346, 2398, 2732
BmgBI CACGTC 2 cut(s) 2266, 2323
BmrI ACTGGG 5 cut(s) 398, 470, 2525, 2815, 2940
BmsI GCATC 2 cut(s) 2065, 3258
BmtI GCTAGC 1 cut(s) 3216
BmuI ACTGGG 5 cut(s) 398, 470, 2525, 2815, 2940
BplI GAGNNNNNCTC 2 cut(s) 2429, 2461
BpmI CTGGAG 7 cut(s) 482, 612, 1274, 1315, 1571, 1904, 3234
Bpu10I CCTNAGC 4 cut(s) 374, 426, 446, 1820
Bpu1102I GCTNAGC 3 cut(s) 143, 533, 664
Bpu14I TTCGAA 1 cut(s) 1131
Bsa29I ATCGAT 1 cut(s) 1205
BsaAI YACGTR 1 cut(s) 2386
BsaHI GRCGYC 3 cut(s) 1652, 2339, 2373
BsaI GGTCTC 2 cut(s) 1908, 2985
BsaXI ACNNNNNCTCC 6 cut(s) 803, 833, 1313, 1343, 1820, 1850
Bse118I RCCGGY 9 cut(s) 320, 456, 1074, 1259, 1499, 1683, 1691, 2057, 2689
Bse21I CCTNAGG 1 cut(s) 135
Bse3DI GCAATG 2 cut(s) 2844, 3283
BseAI TCCGGA 2 cut(s) 1910, 2242
BseBI CCWGG 5 cut(s) 56, 95, 262, 1635, 2211
BseCI ATCGAT 1 cut(s) 1205
BseGI GGATG 3 cut(s) 2181, 2713, 3148
BseMI GCAATG 2 cut(s) 2844, 3283
BsePI GCGCGC 1 cut(s) 2117
BseRI GAGGAG 4 cut(s) 701, 1354, 2129, 2150
BseSI GKGCMC 4 cut(s) 261, 2206, 2230, 2273
BseX3I CGGCCG 2 cut(s) 1138, 2811
BseXI GCAGC 7 cut(s) 408, 1072, 1429, 1490, 2073, 2201, 3136
BseYI CCCAGC 1 cut(s) 2520
BsgI GTGCAG 4 cut(s) 625, 769, 2128, 2137
Bsh1236I CGCG 9 cut(s) 1986, 2009, 2117, 2119, 2121, 2435, 2629, 3185, 3221
Bsh1285I CGRYCG 3 cut(s) 1141, 2316, 2814
BshNI GGYRCC 1 cut(s) 256
BshTI ACCGGT 2 cut(s) 1259, 1683
BshVI ATCGAT 1 cut(s) 1205
BsiEI CGRYCG 3 cut(s) 1141, 2316, 2814
BsiHKAI GWGCWC 4 cut(s) 777, 795, 921, 1020
BsiHKCI CYCGRG 4 cut(s) 722, 1346, 2398, 2732
BslFI GGGAC 8 cut(s) 167, 812, 1248, 1636, 2080, 2415, 2636, 3114
BsmAI GTCTC 8 cut(s) 125, 719, 757, 774, 1587, 1908, 1966, 2985
BsmBI CGTCTC 2 cut(s) 757, 1966
BsmFI GGGAC 8 cut(s) 167, 812, 1248, 1636, 2080, 2415, 2636, 3114
BsmI GAATGC 3 cut(s) 978, 1531, 1881
Bso31I GGTCTC 2 cut(s) 1908, 2985
BsoBI CYCGRG 4 cut(s) 722, 1346, 2398, 2732
Bsp119I TTCGAA 1 cut(s) 1131
Bsp120I GGGCCC 3 cut(s) 2202, 2226, 2269
Bsp13I TCCGGA 2 cut(s) 1910, 2242
Bsp1720I GCTNAGC 3 cut(s) 143, 533, 664
Bsp19I CCATGG 1 cut(s) 14
Bsp68I TCGCGA 1 cut(s) 2009
BspDI ATCGAT 1 cut(s) 1205
BspEI TCCGGA 2 cut(s) 1910, 2242
BspFNI CGCG 9 cut(s) 1986, 2009, 2117, 2119, 2121, 2435, 2629, 3185, 3221
BspHI TCATGA 1 cut(s) 625
BspMAI CTGCAG 1 cut(s) 1564
BspMI ACCTGC 2 cut(s) 203, 1536
BspOI GCTAGC 1 cut(s) 3216
BspQI GCTCTTC 2 cut(s) 1430, 2996
BspT104I TTCGAA 1 cut(s) 1131
BspT107I GGYRCC 1 cut(s) 256
BspTNI GGTCTC 2 cut(s) 1908, 2985
BsrBI CCGCTC 1 cut(s) 393
BsrDI GCAATG 2 cut(s) 2844, 3283
BsrFI RCCGGY 9 cut(s) 320, 456, 1074, 1259, 1499, 1683, 1691, 2057, 2689
BssAI RCCGGY 9 cut(s) 320, 456, 1074, 1259, 1499, 1683, 1691, 2057, 2689
BssHII GCGCGC 1 cut(s) 2117
BssNI GRCGYC 3 cut(s) 1652, 2339, 2373
BssSI CACGAG 3 cut(s) 333, 2231, 2594
BssT1I CCWWGG 4 cut(s) 14, 703, 1744, 3043
Bst2BI CACGAG 3 cut(s) 333, 2231, 2594
Bst2UI CCWGG 5 cut(s) 56, 95, 262, 1635, 2211
Bst4CI ACNGT 7 cut(s) 1217, 1317, 1734, 2277, 2498, 2550, 2801
Bst6I CTCTTC 6 cut(s) 32, 49, 1169, 1430, 2996, 3307
BstACI GRCGYC 3 cut(s) 1652, 2339, 2373
BstAPI GCANNNNNTGC 2 cut(s) 2067, 2151
BstBAI YACGTR 1 cut(s) 2386
BstBI TTCGAA 1 cut(s) 1131
BstDSI CCRYGG 2 cut(s) 14, 2276
BstEII GGTNACC 1 cut(s) 190
BstF5I GGATG 3 cut(s) 2181, 2713, 3148
BstFNI CGCG 9 cut(s) 1986, 2009, 2117, 2119, 2121, 2435, 2629, 3185, 3221
BstH2I RGCGCY 2 cut(s) 2174, 3086
BstHHI GCGC 9 cut(s) 1060, 1986, 2072, 2119, 2121, 2173, 2606, 2851, 3085
BstMAI GTCTC 8 cut(s) 125, 719, 757, 774, 1587, 1908, 1966, 2985
BstMCI CGRYCG 3 cut(s) 1141, 2316, 2814
BstNI CCWGG 5 cut(s) 56, 95, 262, 1635, 2211
BstNSI RCATGY 2 cut(s) 1939, 2975
BstPI GGTNACC 1 cut(s) 190
BstSFI CTRYAG 1 cut(s) 1560
BstSLI GKGCMC 4 cut(s) 261, 2206, 2230, 2273
BstUI CGCG 9 cut(s) 1986, 2009, 2117, 2119, 2121, 2435, 2629, 3185, 3221
BstV1I GCAGC 7 cut(s) 408, 1072, 1429, 1490, 2073, 2201, 3136
BstX2I RGATCY 4 cut(s) 411, 1050, 3013, 3275
BstXI CCANNNNNNTGG 3 cut(s) 180, 2828, 3086
BstYI RGATCY 4 cut(s) 411, 1050, 3013, 3275
BstZI CGGCCG 2 cut(s) 1138, 2811
Bsu15I ATCGAT 1 cut(s) 1205
Bsu36I CCTNAGG 1 cut(s) 135
BsuI GTATCC 1 cut(s) 2481
BsuTUI ATCGAT 1 cut(s) 1205
BtgI CCRYGG 2 cut(s) 14, 2276
BtgZI GCGATG 1 cut(s) 2141
BtrI CACGTC 2 cut(s) 2266, 2323
BtsCI GGATG 3 cut(s) 2181, 2713, 3148
BtsIMutI CAGTG 6 cut(s) 365, 386, 1799, 2072, 2987, 3249
BtuMI TCGCGA 1 cut(s) 2009
BveI ACCTGC 2 cut(s) 203, 1536
CaiI CAGNNNCTG 1 cut(s) 1738
CciI TCATGA 1 cut(s) 625
CfoI GCGC 9 cut(s) 1060, 1986, 2072, 2119, 2121, 2173, 2606, 2851, 3085
Cfr10I RCCGGY 9 cut(s) 320, 456, 1074, 1259, 1499, 1683, 1691, 2057, 2689
ClaI ATCGAT 1 cut(s) 1205
CpoI CGGWCCG 2 cut(s) 758, 2801
CseI GACGC 4 cut(s) 2014, 2296, 2347, 2362
Csp6I GTAC 3 cut(s) 811, 1767, 3105
CspAI ACCGGT 2 cut(s) 1259, 1683
CspI CGGWCCG 2 cut(s) 758, 2801
CviAII CATG 9 cut(s) 15, 108, 174, 626, 655, 1936, 2029, 2972, 3173
CviQI GTAC 3 cut(s) 811, 1767, 3105
DrdI GACNNNNNNGTC 1 cut(s) 2258
DriI GACNNNNNGTC 1 cut(s) 1629
DseDI GACNNNNNNGTC 1 cut(s) 2258
EaeI YGGCCR 4 cut(s) 1138, 2207, 2811, 2965
EagI CGGCCG 2 cut(s) 1138, 2811
Eam1104I CTCTTC 6 cut(s) 32, 49, 1169, 1430, 2996, 3307
Eam1105I GACNNNNNGTC 1 cut(s) 1629
EarI CTCTTC 6 cut(s) 32, 49, 1169, 1430, 2996, 3307
EciI GGCGGA 3 cut(s) 2437, 2669, 3134
Ecl136II GAGCTC 4 cut(s) 775, 793, 919, 1018
EclXI CGGCCG 2 cut(s) 1138, 2811
Eco130I CCWWGG 4 cut(s) 14, 703, 1744, 3043
Eco31I GGTCTC 2 cut(s) 1908, 2985
Eco47III AGCGCT 1 cut(s) 3084
Eco52I CGGCCG 2 cut(s) 1138, 2811
Eco53kI GAGCTC 4 cut(s) 775, 793, 919, 1018
Eco57I CTGAAG 5 cut(s) 524, 995, 2203, 3225, 3291
Eco72I CACGTG 1 cut(s) 2386
Eco81I CCTNAGG 1 cut(s) 135
Eco88I CYCGRG 4 cut(s) 722, 1346, 2398, 2732
Eco91I GGTNACC 1 cut(s) 190
EcoICRI GAGCTC 4 cut(s) 775, 793, 919, 1018
EcoO109I RGGNCCY 3 cut(s) 154, 614, 2226
EcoO65I GGTNACC 1 cut(s) 190
EcoRI GAATTC 2 cut(s) 961, 1133
EcoRII CCWGG 5 cut(s) 54, 93, 260, 1633, 2209
EcoT14I CCWWGG 4 cut(s) 14, 703, 1744, 3043
ErhI CCWWGG 4 cut(s) 14, 703, 1744, 3043
Esp3I CGTCTC 2 cut(s) 757, 1966
FaeI CATG 9 cut(s) 18, 111, 177, 629, 658, 1939, 2032, 2975, 3176
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FaqI GGGAC 8 cut(s) 167, 812, 1248, 1636, 2080, 2415, 2636, 3114
FatI CATG 9 cut(s) 14, 107, 173, 625, 654, 1935, 2028, 2971, 3172
FauI CCCGC 9 cut(s) 170, 530, 1350, 1422, 1742, 2105, 2193, 2216, 3190
FbaI TGATCA 2 cut(s) 3031, 3175
FblI GTMKAC 1 cut(s) 2262
FokI GGATG 3 cut(s) 2168, 2720, 3155
FspBI CTAG 5 cut(s) 248, 549, 1064, 2775, 3213
FspI TGCGCA 1 cut(s) 2605
GlaI GCGC 9 cut(s) 1059, 1985, 2071, 2118, 2120, 2172, 2605, 2850, 3084
GsaI CCCAGC 1 cut(s) 2524
GsuI CTGGAG 7 cut(s) 482, 612, 1274, 1315, 1571, 1904, 3234
HaeII RGCGCY 2 cut(s) 2174, 3086
HgaI GACGC 4 cut(s) 2014, 2296, 2347, 2362
HhaI GCGC 9 cut(s) 1060, 1986, 2072, 2119, 2121, 2173, 2606, 2851, 3085
Hin1I GRCGYC 3 cut(s) 1652, 2339, 2373
Hin1II CATG 9 cut(s) 18, 111, 177, 629, 658, 1939, 2032, 2975, 3176
Hin6I GCGC 9 cut(s) 1058, 1984, 2070, 2117, 2119, 2171, 2604, 2849, 3083
HinP1I GCGC 9 cut(s) 1058, 1984, 2070, 2117, 2119, 2171, 2604, 2849, 3083
HincII GTYRAC 2 cut(s) 1709, 2263
HindII GTYRAC 2 cut(s) 1709, 2263
HindIII AAGCTT 2 cut(s) 1181, 1304
Hpy166II GTNNAC 4 cut(s) 61, 1709, 2263, 2380
Hpy8I GTNNAC 4 cut(s) 61, 1709, 2263, 2380
Hpy99I CGWCG 6 cut(s) 1598, 1952, 2267, 2324, 2378, 2966
HpyCH4III ACNGT 7 cut(s) 1217, 1317, 1734, 2277, 2498, 2550, 2801
HpyCH4IV ACGT 8 cut(s) 1625, 1652, 1959, 2265, 2322, 2349, 2385, 2491
HpySE526I ACGT 8 cut(s) 1625, 1652, 1959, 2265, 2322, 2349, 2385, 2491
Hsp92I GRCGYC 3 cut(s) 1652, 2339, 2373
Hsp92II CATG 9 cut(s) 18, 111, 177, 629, 658, 1939, 2032, 2975, 3176
HspAI GCGC 9 cut(s) 1058, 1984, 2070, 2117, 2119, 2171, 2604, 2849, 3083
KflI GGGWCCC 1 cut(s) 154
Kpn2I TCCGGA 2 cut(s) 1910, 2242
KroI GCCGGC 3 cut(s) 1074, 1499, 2689
KroNI GCCGGC 3 cut(s) 1076, 1501, 2691
Ksp22I TGATCA 2 cut(s) 3031, 3175
LguI GCTCTTC 2 cut(s) 1430, 2996
Lsp1109I GCAGC 7 cut(s) 408, 1072, 1429, 1490, 2073, 2201, 3136
LweI GCATC 2 cut(s) 2065, 3258
MaeI CTAG 5 cut(s) 248, 549, 1064, 2775, 3213
MaeII ACGT 8 cut(s) 1625, 1652, 1959, 2265, 2322, 2349, 2385, 2491
MauBI CGCGCGCG 1 cut(s) 2117
MbiI CCGCTC 1 cut(s) 393
MfeI CAATTG 3 cut(s) 148, 925, 1022
MflI RGATCY 4 cut(s) 411, 1050, 3013, 3275
MlyI GAGTC 6 cut(s) 127, 1632, 1639, 1714, 2397, 3241
MmeI TCCRAC 1 cut(s) 1236
MroI TCCGGA 2 cut(s) 1910, 2242
MroNI GCCGGC 3 cut(s) 1074, 1499, 2689
MroXI GAANNNNTTC 2 cut(s) 1856, 2423
MseI TTAA 3 cut(s) 35, 351, 1956
MslI CAYNNNNRTG 3 cut(s) 178, 956, 2706
MspA1I CMGCKG 3 cut(s) 537, 2223, 3311
MunI CAATTG 3 cut(s) 148, 925, 1022
Mva1269I GAATGC 3 cut(s) 978, 1531, 1881
MvaI CCWGG 5 cut(s) 56, 95, 262, 1635, 2211
MvnI CGCG 9 cut(s) 1986, 2009, 2117, 2119, 2121, 2435, 2629, 3185, 3221
NaeI GCCGGC 3 cut(s) 1076, 1501, 2691
NcoI CCATGG 1 cut(s) 14
NgoMIV GCCGGC 3 cut(s) 1074, 1499, 2689
NheI GCTAGC 1 cut(s) 3212
NlaIII CATG 9 cut(s) 18, 111, 177, 629, 658, 1939, 2032, 2975, 3176
NmeAIII GCCGAG 3 cut(s) 900, 1857, 2625
NmuCI GTSAC 6 cut(s) 190, 929, 1653, 2284, 2492, 3244
NruI TCGCGA 1 cut(s) 2009
NsbI TGCGCA 1 cut(s) 2605
NspI RCATGY 2 cut(s) 1939, 2975
NspV TTCGAA 1 cut(s) 1131
PaeR7I CTCGAG 1 cut(s) 722
PagI TCATGA 1 cut(s) 625
PaqCI CACCTGC 1 cut(s) 203
PauI GCGCGC 1 cut(s) 2117
PciSI GCTCTTC 2 cut(s) 1430, 2996
PcsI WCGNNNNNNNCGW 3 cut(s) 1474, 1956, 2382
PctI GAATGC 3 cut(s) 978, 1531, 1881
PdiI GCCGGC 3 cut(s) 1076, 1501, 2691
PdmI GAANNNNTTC 2 cut(s) 1856, 2423
PfeI GAWTC 9 cut(s) 1123, 1294, 1372, 1465, 1510, 1792, 2424, 2977, 3025
PflFI GACNNNGTC 1 cut(s) 2348
PflMI CCANNNNNTGG 2 cut(s) 704, 940
PfoI TCCNGGA 2 cut(s) 795, 1633
PinAI ACCGGT 2 cut(s) 1259, 1683
Ple19I CGATCG 1 cut(s) 2316
PleI GAGTC 6 cut(s) 126, 1632, 1638, 1713, 2397, 3241
PmaCI CACGTG 1 cut(s) 2386
PmlI CACGTG 1 cut(s) 2386
PpsI GAGTC 6 cut(s) 126, 1632, 1638, 1713, 2397, 3241
Ppu21I YACGTR 1 cut(s) 2386
PpuMI RGGWCCY 2 cut(s) 154, 614
Psp124BI GAGCTC 4 cut(s) 777, 795, 921, 1020
Psp5II RGGWCCY 2 cut(s) 154, 614
Psp6I CCWGG 5 cut(s) 54, 93, 260, 1633, 2209
PspCI CACGTG 1 cut(s) 2386
PspEI GGTNACC 1 cut(s) 190
PspFI CCCAGC 1 cut(s) 2520
PspGI CCWGG 5 cut(s) 54, 93, 260, 1633, 2209
PspOMI GGGCCC 3 cut(s) 2202, 2226, 2269
PspPPI RGGWCCY 2 cut(s) 154, 614
PstI CTGCAG 1 cut(s) 1564
PstNI CAGNNNCTG 1 cut(s) 1738
PsuI RGATCY 4 cut(s) 411, 1050, 3013, 3275
PsyI GACNNNGTC 1 cut(s) 2348
PteI GCGCGC 1 cut(s) 2117
PvuI CGATCG 1 cut(s) 2316
PvuII CAGCTG 1 cut(s) 3311
RruI TCGCGA 1 cut(s) 2009
RsaI GTAC 3 cut(s) 812, 1768, 3106
RsaNI GTAC 3 cut(s) 811, 1767, 3105
RseI CAYNNNNRTG 3 cut(s) 178, 956, 2706
Rsr2I CGGWCCG 2 cut(s) 758, 2801
RsrII CGGWCCG 2 cut(s) 758, 2801
SacI GAGCTC 4 cut(s) 777, 795, 921, 1020
SalI GTCGAC 1 cut(s) 2261
SapI GCTCTTC 2 cut(s) 1430, 2996
SaqAI TTAA 3 cut(s) 35, 351, 1956
SbfI CCTGCAGG 1 cut(s) 1564
SchI GAGTC 6 cut(s) 127, 1632, 1639, 1714, 2397, 3241
SdaI CCTGCAGG 1 cut(s) 1564
SfaNI GCATC 2 cut(s) 2065, 3258
SfcI CTRYAG 1 cut(s) 1560
Sfr274I CTCGAG 1 cut(s) 722
SfuI TTCGAA 1 cut(s) 1131
SlaI CTCGAG 1 cut(s) 722
SmiMI CAYNNNNRTG 3 cut(s) 178, 956, 2706
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
Sse8387I CCTGCAGG 1 cut(s) 1564
SspI AATATT 1 cut(s) 2725
SspMI CTAG 5 cut(s) 248, 549, 1064, 2775, 3213
SstI GAGCTC 4 cut(s) 777, 795, 921, 1020
StyI CCWWGG 4 cut(s) 14, 703, 1744, 3043
TaaI ACNGT 7 cut(s) 1217, 1317, 1734, 2277, 2498, 2550, 2801
TaiI ACGT 8 cut(s) 1628, 1655, 1962, 2268, 2325, 2352, 2388, 2494
TfiI GAWTC 9 cut(s) 1123, 1294, 1372, 1465, 1510, 1792, 2424, 2977, 3025
Tru1I TTAA 3 cut(s) 35, 351, 1956
Tru9I TTAA 3 cut(s) 35, 351, 1956
TscAI CASTG 6 cut(s) 372, 393, 1806, 2072, 2987, 3249
TseFI GTSAC 6 cut(s) 190, 929, 1653, 2284, 2492, 3244
TseI GCWGC 7 cut(s) 421, 1060, 1417, 1503, 2061, 2189, 3149
Tsp45I GTSAC 6 cut(s) 190, 929, 1653, 2284, 2492, 3244
TspDTI ATGAA 5 cut(s) 96, 180, 974, 1191, 3152
TspGWI ACGGA 2 cut(s) 2417, 2793
TspRI CASTG 6 cut(s) 372, 393, 1806, 2072, 2987, 3249
Tth111I GACNNNGTC 1 cut(s) 2348
Van91I CCANNNNNTGG 2 cut(s) 704, 940
XapI RAATTY 9 cut(s) 226, 265, 543, 861, 961, 1133, 1444, 3090, 3165
XceI RCATGY 2 cut(s) 1939, 2975
XcmI CCANNNNNNNNNTGG 1 cut(s) 1669
XhoI CTCGAG 1 cut(s) 722
XmiI GTMKAC 1 cut(s) 2262
XmnI GAANNNNTTC 2 cut(s) 1856, 2423
XspI CTAG 5 cut(s) 248, 549, 1064, 2775, 3213
ZraI GACGTC 1 cut(s) 1653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.