FvH4_5g06010

rpm1,rps3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0019233
Physical Location & Seq
Forward (+)
3525698 .. 3527260
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06010.t1

Sequence Viewer

Length: 1563 bp
ATGGAAGAAAGCAAGGACAGCCATGAAGATGAAAAAGGTGATACATCAACTTCTTCTTCACCTTGTCCATTACAGTTACGTTACATAGAGTTACCTTATCACCTTCAAAGTTGTGTAGTGTACTGTTCCATCTTCCCTGAGAGCTACCGGCTTTCAAAAGGGAAACTTATCCGGTTACTAGTAGCTCAAGGACTGATACAAGAAAAAGCAGGGAAGATTATGGAGGATGTAGCAGAAGAATATATATGTGAACTGATCAACCAGCAACTGCTTCAAGTACTGGAGGAACACCCTCGGAGAGATGGGACTCAATTGAGGGTCCCCTGTGGTCTTCAAAAGTTCTGTCTTGATCAACTGAAGGAAGGAAAATTTGCAACCTTTGGAGTTTTCATTTGTTCAGATATCACACAGCTGATATCCCAAATGAACAATCTTCAACTAACTGCTTTGTTCCTGCTAGCAAATCAGAATCTATCTCAACATGATGGTAGTTGGCTAAAATTTAATGGTGTGAACACTTTAAAAGTGCTTGAGCTAAAAGATACCAAAATCAAGAAGTTACCAGATGAAGTGGGAGACCTAATACACCTCTCGTATCTTGGCTTGAAAAGCACGGATATAAATGAGCTTCCGGAGGGGATAGGCAGATTAGAAGCCCTGCAATCTTTGGATATTAGATGGTGTGGGGATTTAACAGCATTGCCAGCTGAGATACTCAACCTTGTAAGGTTGAGGCACCTCAAAATGTTCAAGAACAAAGGCGTTTCTGGGATGAAGCTACCGGCAGGGATAGGAAGACTTGCAGATCTTCTGACCTTGACAGGTATACATGCTGGTAGTGGCACTGCAGCTGAAGTAGGTGACCTGATAGGGCTCAGAAGATTAGGAGTGATGGACGTGGACGAGGAGAATTTCACTGAGCTGTTTGCCTCTATTATGAAGATGCAGGGCCTTCTTTCCTTGTCTTTAGAAGCAAAATGCGCATTCCCTCAGGGAGAGCTTTTCCTCCTGGAGTCATTCTCACCTCCACCATTTCTTCAAAAGCTTCGCCTTGAGGGCATCCTACGAAAGCTACCTGCTTGGGTTGGTTGCTTGGAAAGGCTCACGAACTTAAGATTAGGTTTCTCCAATCTGTCTGAGAACCCATCATTGGTACTTCAAGTCCTGCCCAACTTGAAAAAGTTAACCCTTTGGCAGGCTTACGATGCGAAGCAAATAGGGAAAGAGTTCTGTACTGCTGGTGGGTTTCCAAAGCTCGAGGTTCTCATCATTGCTTCTCGTGTTCTTGAGGAGTGGACAGAACTTGAAGAGGGAGCACTACCTAGCTTGAAGTACCTCCACTTCCACAATTGTCTGCGGCTGAGGATGTTACCTGAAGGTTTGCAATCCCTCACCACACTGAAGCGTTTGCATTTGCTGCCTCTGCTGGATGATCATGCAGAACGGTTGAAACCTGATGGTGGAGAGGAGTATTACAAAATTAGGCACATTCCAGAGACATCATACACTACCAACTCTATGATACAAAGATATGTGCAAGCTGCGCAAAGAGGAACAACATGA

Protein Analysis

521

Amino Acids

58.45

Weight (kDa)

6.12

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 44 - 97 2.5e-13 Disease resistance protein Winged helix domain
LRR_14 PF23598 166 - 471 2.8e-42 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06010 FvH4_5g32740
malus_domestica MD14G1174200.v1.1
prunus_persica Prupe.5G167700_v2.0.a1
rosa_multiflora Rmu_sc0002040.1_g000041 Rmu_sc0028138.1_g000001 Rmu_sc0034887.1_g000001
rosa_rugosa Rorug07G0005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 982, 1545
Acc36I ACCTGC 1 cut(s) 1084
AccB1I GGYRCC 1 cut(s) 735
AccI GTMKAC 1 cut(s) 826
AccIII TCCGGA 1 cut(s) 631
AciI CCGC 1 cut(s) 1357
AcsI RAATTY 3 cut(s) 368, 500, 910
AcuI CTGAAG 4 cut(s) 377, 873, 1395, 1421
AfaI GTAC 5 cut(s) 122, 279, 1155, 1234, 1334
AfiI CCNNNNNNNGG 3 cut(s) 1150, 1195, 1460
AflII CTTAAG 1 cut(s) 1111
AhlI ACTAGT 1 cut(s) 178
AjiI CACGTC 1 cut(s) 898
AjnI CCWGG 1 cut(s) 1008
AjuI GAANNNNNNNTTGG 2 cut(s) 1133, 1165
Alw21I GWGCWC 1 cut(s) 1318
Alw26I GTCTC 2 cut(s) 570, 1490
AlwNI CAGNNNCTG 1 cut(s) 268
Ama87I CYCGRG 1 cut(s) 1256
Aor13HI TCCGGA 1 cut(s) 631
AoxI GGCC 1 cut(s) 949
ApeKI GCWGC 3 cut(s) 848, 1417, 1541
ApoI RAATTY 3 cut(s) 368, 500, 910
ArsI GACNNNNNNTTYG 2 cut(s) 328, 360
Asp700I GAANNNNTTC 1 cut(s) 1226
AspLEI GCGC 2 cut(s) 983, 1546
AspS9I GGNCC 2 cut(s) 319, 949
AsuHPI GGTGA 6 cut(s) 50, 51, 92, 872, 1014, 1384
AsuNHI GCTAGC 1 cut(s) 457
AvaI CYCGRG 1 cut(s) 1256
AvaII GGWCC 1 cut(s) 319
AxyI CCTNAGG 1 cut(s) 990
BanI GGYRCC 1 cut(s) 735
BanII GRGCYC 1 cut(s) 876
BarI GAAGNNNNNNTAC 2 cut(s) 1325, 1357
BauI CACGAG 1 cut(s) 1278
BbsI GAAGAC 2 cut(s) 323, 802
Bbv12I GWGCWC 1 cut(s) 1318
BbvCI CCTCAGC 1 cut(s) 1361
BbvI GCAGC 3 cut(s) 860, 1404, 1528
BccI CCATC 7 cut(s) 137, 296, 479, 672, 886, 1153, 1451
BciT130I CCWGG 1 cut(s) 1010
BclI TGATCA 3 cut(s) 255, 349, 1432
BcoDI GTCTC 2 cut(s) 570, 1490
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 3 cut(s) 179, 458, 1323
BfmI CTRYAG 1 cut(s) 846
BfrI CTTAAG 1 cut(s) 1111
BfuAI ACCTGC 1 cut(s) 1084
BglI GCCNNNNNGGC 1 cut(s) 1056
BglII AGATCT 1 cut(s) 805
BisI GCNGC 4 cut(s) 849, 1358, 1418, 1542
BlsI GCNGC 4 cut(s) 850, 1359, 1419, 1543
BmcAI AGTACT 1 cut(s) 279
Bme1390I CCNGG 1 cut(s) 1010
Bme18I GGWCC 1 cut(s) 319
BmeT110I CYCGRG 1 cut(s) 1256
BmgBI CACGTC 1 cut(s) 898
BmgT120I GGNCC 2 cut(s) 319, 949
BmiI GGNNCC 3 cut(s) 320, 321, 737
BmrFI CCNGG 1 cut(s) 1010
BmsI GCATC 3 cut(s) 933, 1068, 1195
BmtI GCTAGC 1 cut(s) 461
BpiI GAAGAC 2 cut(s) 323, 802
BplI GAGNNNNNCTC 2 cut(s) 1004, 1036
BpmI CTGGAG 2 cut(s) 302, 1031
Bpu10I CCTNAGC 1 cut(s) 1361
BpuEI CTTGAG 4 cut(s) 171, 551, 1073, 1307
BsaI GGTCTC 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 293
BsaWI WCCGGW 2 cut(s) 171, 631
Bsc4I CCNNNNNNNGG 3 cut(s) 1150, 1195, 1460
Bse118I RCCGGY 2 cut(s) 147, 781
Bse1I ACTGG 1 cut(s) 285
Bse21I CCTNAGG 1 cut(s) 990
Bse3DI GCAATG 2 cut(s) 698, 1269
BseAI TCCGGA 1 cut(s) 631
BseBI CCWGG 1 cut(s) 1010
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 5 cut(s) 232, 777, 1059, 1371, 1435
BseLI CCNNNNNNNGG 3 cut(s) 1150, 1195, 1460
BseMI GCAATG 2 cut(s) 698, 1269
BseMII CTCAG 7 cut(s) 129, 699, 889, 909, 1004, 1128, 1352
BseNI ACTGG 1 cut(s) 285
BseRI GAGGAG 3 cut(s) 920, 1304, 1481
BseXI GCAGC 3 cut(s) 860, 1404, 1528
BshFI GGCC 1 cut(s) 951
BshNI GGYRCC 1 cut(s) 735
BsiHKAI GWGCWC 1 cut(s) 1318
BsiHKCI CYCGRG 1 cut(s) 1256
BsiSI CCGG 4 cut(s) 148, 172, 632, 782
BslFI GGGAC 2 cut(s) 305, 319
BslI CCNNNNNNNGG 3 cut(s) 1150, 1195, 1460
BsmAI GTCTC 2 cut(s) 570, 1490
BsmFI GGGAC 2 cut(s) 305, 319
BsmI GAATGC 1 cut(s) 983
BsnI GGCC 1 cut(s) 951
Bso31I GGTCTC 1 cut(s) 570
BsoBI CYCGRG 1 cut(s) 1256
Bsp1286I GDGCHC 2 cut(s) 876, 1318
Bsp13I TCCGGA 1 cut(s) 631
Bsp143I GATC 4 cut(s) 255, 349, 805, 1432
BspACI CCGC 1 cut(s) 1357
BspANI GGCC 1 cut(s) 951
BspCNI CTCAG 7 cut(s) 130, 700, 888, 910, 1003, 1129, 1353
BspEI TCCGGA 1 cut(s) 631
BspLI GGNNCC 3 cut(s) 320, 321, 737
BspMAI CTGCAG 1 cut(s) 850
BspMI ACCTGC 1 cut(s) 1084
BspOI GCTAGC 1 cut(s) 461
BspT107I GGYRCC 1 cut(s) 735
BspTI CTTAAG 1 cut(s) 1111
BspTNI GGTCTC 1 cut(s) 570
BsrDI GCAATG 2 cut(s) 698, 1269
BsrFI RCCGGY 2 cut(s) 147, 781
BsrI ACTGG 1 cut(s) 285
BssAI RCCGGY 2 cut(s) 147, 781
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 4 cut(s) 255, 349, 805, 1432
BssNAI GTATAC 1 cut(s) 827
BssSI CACGAG 1 cut(s) 1278
Bst1107I GTATAC 1 cut(s) 827
Bst2BI CACGAG 1 cut(s) 1278
Bst2UI CCWGG 1 cut(s) 1010
Bst4CI ACNGT 3 cut(s) 75, 125, 1446
Bst6I CTCTTC 1 cut(s) 1302
BstAFI CTTAAG 1 cut(s) 1111
BstAPI GCANNNNNTGC 1 cut(s) 1417
BstC8I GCNNGC 4 cut(s) 459, 705, 1197, 1539
BstDEI CTNAG 7 cut(s) 138, 708, 875, 918, 990, 1137, 1361
BstEII GGTNACC 1 cut(s) 860
BstENI CCTNNNNNAGG 1 cut(s) 1193
BstF5I GGATG 5 cut(s) 232, 777, 1059, 1371, 1435
BstHHI GCGC 2 cut(s) 983, 1546
BstKTI GATC 4 cut(s) 258, 352, 808, 1435
BstMAI GTCTC 2 cut(s) 570, 1490
BstMBI GATC 4 cut(s) 255, 349, 805, 1432
BstMWI GCNNNNNNNGC 9 cut(s) 18, 609, 704, 980, 1056, 1205, 1417, 1423, 1543
BstNI CCWGG 1 cut(s) 1010
BstNSI RCATGY 1 cut(s) 833
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 1 cut(s) 1008
BstSFI CTRYAG 1 cut(s) 846
BstV1I GCAGC 3 cut(s) 860, 1404, 1528
BstV2I GAAGAC 2 cut(s) 323, 802
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
BstZ17I GTATAC 1 cut(s) 827
Bsu36I CCTNAGG 1 cut(s) 990
BsuRI GGCC 1 cut(s) 951
BtrI CACGTC 1 cut(s) 898
BtsCI GGATG 5 cut(s) 232, 777, 1059, 1371, 1435
BtsI GCAGTG 1 cut(s) 843
BtsIMutI CAGTG 3 cut(s) 843, 915, 1397
BveI ACCTGC 1 cut(s) 1084
Cac8I GCNNGC 4 cut(s) 459, 705, 1197, 1539
CaiI CAGNNNCTG 1 cut(s) 268
CfoI GCGC 2 cut(s) 983, 1546
Cfr10I RCCGGY 2 cut(s) 147, 781
Cfr13I GGNCC 2 cut(s) 319, 949
Csp6I GTAC 5 cut(s) 121, 278, 1154, 1233, 1333
CviAII CATG 5 cut(s) 23, 482, 830, 1436, 1560
CviQI GTAC 5 cut(s) 121, 278, 1154, 1233, 1333
DdeI CTNAG 7 cut(s) 138, 708, 875, 918, 990, 1137, 1361
DpnI GATC 4 cut(s) 257, 351, 807, 1434
DpnII GATC 4 cut(s) 255, 349, 805, 1432
DraI TTTAAA 1 cut(s) 522
Eam1104I CTCTTC 1 cut(s) 1302
EarI CTCTTC 1 cut(s) 1302
Eco24I GRGCYC 1 cut(s) 876
Eco31I GGTCTC 1 cut(s) 570
Eco32I GATATC 2 cut(s) 403, 417
Eco47I GGWCC 1 cut(s) 319
Eco57I CTGAAG 4 cut(s) 377, 873, 1395, 1421
Eco81I CCTNAGG 1 cut(s) 990
Eco88I CYCGRG 1 cut(s) 1256
Eco91I GGTNACC 1 cut(s) 860
EcoNI CCTNNNNNAGG 1 cut(s) 1193
EcoO109I RGGNCCY 2 cut(s) 319, 949
EcoO65I GGTNACC 1 cut(s) 860
EcoRII CCWGG 1 cut(s) 1008
EcoRV GATATC 2 cut(s) 403, 417
EcoT38I GRGCYC 1 cut(s) 876
FaeI CATG 5 cut(s) 26, 485, 833, 1439, 1563
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 2 cut(s) 305, 319
FatI CATG 5 cut(s) 22, 481, 829, 1435, 1559
FbaI TGATCA 3 cut(s) 255, 349, 1432
FblI GTMKAC 1 cut(s) 826
Fnu4HI GCNGC 4 cut(s) 849, 1358, 1418, 1542
FokI GGATG 5 cut(s) 239, 784, 1046, 1378, 1442
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 4 cut(s) 849, 1358, 1418, 1542
FspAI RTGCGCAY 1 cut(s) 982
FspBI CTAG 3 cut(s) 179, 458, 1323
FspI TGCGCA 2 cut(s) 982, 1545
GlaI GCGC 2 cut(s) 982, 1545
GluI GCNGC 4 cut(s) 849, 1358, 1418, 1542
GsuI CTGGAG 2 cut(s) 302, 1031
HaeIII GGCC 1 cut(s) 951
HapII CCGG 4 cut(s) 148, 172, 632, 782
HhaI GCGC 2 cut(s) 983, 1546
Hin1II CATG 5 cut(s) 26, 485, 833, 1439, 1563
Hin6I GCGC 2 cut(s) 981, 1544
HinP1I GCGC 2 cut(s) 981, 1544
HincII GTYRAC 1 cut(s) 1185
HindII GTYRAC 1 cut(s) 1185
HindIII AAGCTT 1 cut(s) 1043
HinfI GANTC 3 cut(s) 307, 469, 1013
HpaI GTTAAC 1 cut(s) 1185
HpaII CCGG 4 cut(s) 148, 172, 632, 782
HphI GGTGA 6 cut(s) 50, 51, 92, 872, 1014, 1384
Hpy166II GTNNAC 7 cut(s) 121, 251, 514, 827, 901, 1185, 1296
Hpy188I TCNGA 6 cut(s) 297, 400, 468, 813, 878, 1138
Hpy188III TCNNGA 7 cut(s) 347, 553, 632, 751, 1105, 1286, 1493
Hpy8I GTNNAC 7 cut(s) 121, 251, 514, 827, 901, 1185, 1296
HpyAV CCTTC 5 cut(s) 113, 352, 356, 962, 1370
HpyCH4III ACNGT 3 cut(s) 75, 125, 1446
HpyCH4IV ACGT 2 cut(s) 79, 897
HpyCH4V TGCA 9 cut(s) 374, 661, 803, 848, 946, 1384, 1411, 1439, 1537
HpyF10VI GCNNNNNNNGC 9 cut(s) 18, 609, 704, 980, 1056, 1205, 1417, 1423, 1543
HpyF3I CTNAG 7 cut(s) 138, 708, 875, 918, 990, 1137, 1361
HpySE526I ACGT 2 cut(s) 79, 897
Hsp92II CATG 5 cut(s) 26, 485, 833, 1439, 1563
HspAI GCGC 2 cut(s) 981, 1544
KflI GGGWCCC 1 cut(s) 319
Kpn2I TCCGGA 1 cut(s) 631
Ksp22I TGATCA 3 cut(s) 255, 349, 1432
KspAI GTTAAC 1 cut(s) 1185
Kzo9I GATC 4 cut(s) 255, 349, 805, 1432
LmnI GCTCC 1 cut(s) 1313
Lsp1109I GCAGC 3 cut(s) 860, 1404, 1528
LweI GCATC 3 cut(s) 933, 1068, 1195
MaeI CTAG 3 cut(s) 179, 458, 1323
MaeII ACGT 2 cut(s) 79, 897
MaeIII GTNAC 7 cut(s) 75, 80, 90, 174, 558, 860, 1368
MalI GATC 4 cut(s) 257, 351, 807, 1434
MboI GATC 4 cut(s) 255, 349, 805, 1432
MfeI CAATTG 2 cut(s) 311, 1348
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 2 cut(s) 876, 1318
MluCI AATT 6 cut(s) 311, 368, 500, 910, 1348, 1479
MlyI GAGTC 2 cut(s) 301, 1022
MroI TCCGGA 1 cut(s) 631
MroXI GAANNNNTTC 1 cut(s) 1226
MseI TTAA 5 cut(s) 504, 521, 692, 1112, 1184
MslI CAYNNNNRTG 1 cut(s) 27
MspA1I CMGCKG 3 cut(s) 412, 707, 851
MspCI CTTAAG 1 cut(s) 1111
MspI CCGG 4 cut(s) 148, 172, 632, 782
MspR9I CCNGG 1 cut(s) 1010
MunI CAATTG 2 cut(s) 311, 1348
Mva1269I GAATGC 1 cut(s) 983
MvaI CCWGG 1 cut(s) 1010
MwoI GCNNNNNNNGC 9 cut(s) 18, 609, 704, 980, 1056, 1205, 1417, 1423, 1543
NdeII GATC 4 cut(s) 255, 349, 805, 1432
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 5 cut(s) 26, 485, 833, 1439, 1563
NlaIV GGNNCC 3 cut(s) 320, 321, 737
NmuCI GTSAC 1 cut(s) 860
NsbI TGCGCA 2 cut(s) 982, 1545
NspI RCATGY 1 cut(s) 833
PaeR7I CTCGAG 1 cut(s) 1256
PctI GAATGC 1 cut(s) 983
PdmI GAANNNNTTC 1 cut(s) 1226
PfeI GAWTC 1 cut(s) 469
PfoI TCCNGGA 1 cut(s) 1008
PkrI GCNGC 4 cut(s) 850, 1359, 1419, 1543
PleI GAGTC 2 cut(s) 301, 1021
PpsI GAGTC 2 cut(s) 301, 1021
PpuMI RGGWCCY 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 1008
PspEI GGTNACC 1 cut(s) 860
PspGI CCWGG 1 cut(s) 1008
PspN4I GGNNCC 3 cut(s) 320, 321, 737
PspPI GGNCC 2 cut(s) 319, 949
PspPPI RGGWCCY 1 cut(s) 319
PspXI VCTCGAGB 1 cut(s) 1256
PstI CTGCAG 1 cut(s) 850
PstNI CAGNNNCTG 1 cut(s) 268
PsuI RGATCY 1 cut(s) 805
PvuII CAGCTG 3 cut(s) 412, 707, 851
RsaI GTAC 5 cut(s) 122, 279, 1155, 1234, 1334
RsaNI GTAC 5 cut(s) 121, 278, 1154, 1233, 1333
RseI CAYNNNNRTG 1 cut(s) 27
SaqAI TTAA 5 cut(s) 504, 521, 692, 1112, 1184
SatI GCNGC 4 cut(s) 849, 1358, 1418, 1542
Sau3AI GATC 4 cut(s) 255, 349, 805, 1432
Sau96I GGNCC 2 cut(s) 319, 949
ScaI AGTACT 1 cut(s) 279
SchI GAGTC 2 cut(s) 301, 1022
ScrFI CCNGG 1 cut(s) 1010
SduI GDGCHC 2 cut(s) 876, 1318
SfaNI GCATC 3 cut(s) 933, 1068, 1195
SfcI CTRYAG 1 cut(s) 846
Sfr274I CTCGAG 1 cut(s) 1256
SinI GGWCC 1 cut(s) 319
SlaI CTCGAG 1 cut(s) 1256
SmiMI CAYNNNNRTG 1 cut(s) 27
SmlI CTYRAG 6 cut(s) 186, 530, 1052, 1111, 1256, 1286
SmoI CTYRAG 6 cut(s) 186, 530, 1052, 1111, 1256, 1286
SpeI ACTAGT 1 cut(s) 178
Sse9I AATT 6 cut(s) 311, 368, 500, 910, 1348, 1479
SsiI CCGC 1 cut(s) 1357
SspMI CTAG 3 cut(s) 179, 458, 1323
StyD4I CCNGG 1 cut(s) 1008
TaaI ACNGT 3 cut(s) 75, 125, 1446
TaiI ACGT 2 cut(s) 82, 900
TaqI TCGA 1 cut(s) 1257
TasI AATT 6 cut(s) 311, 368, 500, 910, 1348, 1479
TatI WGTACW 3 cut(s) 120, 277, 1232
TauI GCSGC 1 cut(s) 1360
TfiI GAWTC 1 cut(s) 469
Tru1I TTAA 5 cut(s) 504, 521, 692, 1112, 1184
Tru9I TTAA 5 cut(s) 504, 521, 692, 1112, 1184
TscAI CASTG 3 cut(s) 850, 922, 1404
TseFI GTSAC 1 cut(s) 860
TseI GCWGC 3 cut(s) 848, 1417, 1541
Tsp45I GTSAC 1 cut(s) 860
TspDTI ATGAA 7 cut(s) 39, 45, 379, 440, 582, 788, 953
TspGWI ACGGA 1 cut(s) 629
TspRI CASTG 3 cut(s) 850, 922, 1404
Vha464I CTTAAG 1 cut(s) 1111
VpaK11BI GGWCC 1 cut(s) 319
XagI CCTNNNNNAGG 1 cut(s) 1193
XapI RAATTY 3 cut(s) 368, 500, 910
XceI RCATGY 1 cut(s) 833
XhoI CTCGAG 1 cut(s) 1256
XmiI GTMKAC 1 cut(s) 826
XmnI GAANNNNTTC 1 cut(s) 1226
XspI CTAG 3 cut(s) 179, 458, 1323
ZrmI AGTACT 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.