FvH4_5g32740

rpm1,rps3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0019233
Physical Location & Seq
Forward (+)
23630008 .. 23631593
1586 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32740.t1

Sequence Viewer

Length: 1449 bp
ATGGAAGAAGGCAAGGACAACCATGAGGTCAAAGGTGAGACATCAGGTTCTCCTCCTCCTCCTCCTCCTCCATCTTGTCATTTCAGGAGGATTCTGGAGGATGTCGCAGAAGAATATATATGTGAATTGATCAACCAGCGACTGCTTCGAGTAAAGGATGAACTTGATCGGAGAAGAGGGACACAGTTAAGGGTCCCCTGTCGTCTTCAAAAGTTCTGTCTTGACCAACTGAAGGAAGGAAAGTTTGCAGCCTTAAAGGTTTTCATTTGTTCAGACATCAGACAACTCATATCTCAAACGAACAATCTTCAACTAACTGCTTTGTTCCTGCTAGAAAAGGAAGATCTCTCTCAACATGATGGTAGTTGGCTACAATTTGATGGTGTGAACACTGTAAGAGTGCTTGAGCTGAAAGATACCAAAATCAAGAAGTTACCAGATGAAGTGGGAGACCTGAAACACCTCAGGTATCTTGGCTTGAAAAGCACCGATATAGAAGAGCTTCCAGAGGGACTAGGCAGATTAGAAGCCTTGCAAACTTTGGATATTAGATGGTGTGGGGATTTAACAGGCTTGTCAGCTGAGATACTCAATCTTGTAAGGTTGAGGCACGTCAAAATGTACAAGACCAAAGGCGTTTGTGGGATGAAGCTACCAGTAGGAATAGGAAAACTCAGAGATCTTCAGACATTGACAGGTATATACGCTGGTGGTGGAACTGCAGCTGAAGTAGGTAACCTGATAGGGCTCAGAAGACTCGGAGTGATGGATGTGGATGAAGAGAATGTCACTGAGCTATTTGCCTCTATCGAGAAGATGCTGTGCCTTCTTTCCTTGTCTTTAGAAGCAAAGTACAAGTATCCTCAGGAAAAACTTGTCCTCTTGGAGTCATTCTCACCTCCACCATTTCTTCAAAAGCTTCGCCTTGAGGGAATACTAGAAAAGCTACCTGCTTGGTTTGGTTCCTTGGAAAGGCTCACAAGCTTAAGATTAGGTTTCTCCCATTTGTCTGAGAACCCATCATTGGTGCTTCAAGTCCTACTCAACTTGAAAAGCTTAACCCTTTGGGAGGCTTATGATGCAAAGCAAACAGGGAAAGAGTTCTGTGGTGCTGGTGGATTTTTCAAGCTCGAGATTCTCATCATTGCTTCTCCTGTTCTCGAGGAGTGGACAGAACTTGAAGAGGGAGCACTACCTAGCTTGAGGTACCTCCACTTGCACAGTTGTGTGCGCCTGAAAATGTTACCTGAAGGTTTGCAGTCCCTCACCAAACTGGAGCAGTTGCATTTGCTGCCTATGATGGATGATCATGCAGAACGGCTGAAACCTGATGGTGGGGTGGAGTATTACAAAATTAGGCACATTCCAGAGATATCATACATTACCAATTCTATGATACAAGAAATCGTGAAAACTGGAGAAGGAGGAATAGAAATGCTGCAAGGATAA

Protein Analysis

483

Amino Acids

54.43

Weight (kDa)

5.8

Isoelectric Point (pI)

52.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 124 - 429 2.3e-39 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06010 FvH4_5g32740
malus_domestica MD14G1174200.v1.1
prunus_persica Prupe.5G167700_v2.0.a1
rosa_multiflora Rmu_sc0002040.1_g000041 Rmu_sc0028138.1_g000001 Rmu_sc0034887.1_g000001
rosa_rugosa Rorug07G0005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 958
Acc65I GGTACC 1 cut(s) 1206
AccB1I GGYRCC 1 cut(s) 1206
AcuI CTGAAG 4 cut(s) 251, 668, 747, 1269
AfaI GTAC 3 cut(s) 623, 854, 1208
AfiI CCNNNNNNNGG 5 cut(s) 232, 972, 1024, 1069, 1334
AflII CTTAAG 1 cut(s) 985
AgsI TTSAA 8 cut(s) 209, 311, 481, 914, 1034, 1051, 1126, 1181
AjiI CACGTC 1 cut(s) 613
AjuI GAANNNNNNNTTGG 2 cut(s) 1007, 1039
AleI CACNNNNGTG 1 cut(s) 1224
Alw21I GWGCWC 1 cut(s) 1192
Alw26I GTCTC 2 cut(s) 32, 444
AlwNI CAGNNNCTG 1 cut(s) 142
Ama87I CYCGRG 2 cut(s) 1130, 1160
ApeKI GCWGC 4 cut(s) 248, 722, 1291, 1438
ArsI GACNNNNNNTTYG 2 cut(s) 202, 234
Asp700I GAANNNNTTC 2 cut(s) 501, 1100
Asp718I GGTACC 1 cut(s) 1206
AspLEI GCGC 1 cut(s) 1233
AspS9I GGNCC 1 cut(s) 193
AsuHPI GGTGA 3 cut(s) 47, 888, 1258
AvaI CYCGRG 2 cut(s) 1130, 1160
AvaII GGWCC 1 cut(s) 193
AxyI CCTNAGG 2 cut(s) 464, 864
BanI GGYRCC 1 cut(s) 1206
BanII GRGCYC 1 cut(s) 750
BbsI GAAGAC 2 cut(s) 197, 760
Bbv12I GWGCWC 1 cut(s) 1192
BbvI GCAGC 4 cut(s) 260, 734, 1278, 1425
BccI CCATC 8 cut(s) 79, 353, 374, 546, 760, 1027, 1294, 1325
BceAI ACGGC 1 cut(s) 1334
BciVI GTATCC 1 cut(s) 870
BclI TGATCA 2 cut(s) 129, 1306
BcoDI GTCTC 2 cut(s) 32, 444
BfaI CTAG 4 cut(s) 332, 515, 938, 1197
BfmI CTRYAG 1 cut(s) 720
BfrI CTTAAG 1 cut(s) 985
BfuAI ACCTGC 1 cut(s) 958
BfuI GTATCC 1 cut(s) 870
BglII AGATCT 2 cut(s) 343, 679
BisI GCNGC 4 cut(s) 249, 723, 1292, 1439
BlsI GCNGC 4 cut(s) 250, 724, 1293, 1440
Bme18I GGWCC 1 cut(s) 193
BmeT110I CYCGRG 2 cut(s) 1130, 1160
BmgBI CACGTC 1 cut(s) 613
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 4 cut(s) 194, 195, 964, 1208
BmsI GCATC 2 cut(s) 807, 1069
BpiI GAAGAC 2 cut(s) 197, 760
BplI GAGNNNNNCTC 2 cut(s) 878, 910
BpmI CTGGAG 3 cut(s) 116, 1295, 1437
BpuEI CTTGAG 3 cut(s) 425, 947, 1222
BsaBI GATNNNNATC 1 cut(s) 1139
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 1 cut(s) 966
Bsc4I CCNNNNNNNGG 5 cut(s) 232, 972, 1024, 1069, 1334
Bse1I ACTGG 3 cut(s) 656, 1278, 1420
Bse21I CCTNAGG 2 cut(s) 464, 864
Bse3DI GCAATG 1 cut(s) 1143
Bse8I GATNNNNATC 1 cut(s) 1139
BseDI CCNNGG 1 cut(s) 966
BseGI GGATG 6 cut(s) 106, 163, 651, 775, 781, 1309
BseJI GATNNNNATC 1 cut(s) 1139
BseLI CCNNNNNNNGG 5 cut(s) 232, 972, 1024, 1069, 1334
BseMI GCAATG 1 cut(s) 1143
BseMII CTCAG 7 cut(s) 478, 573, 688, 763, 783, 878, 1002
BseNI ACTGG 3 cut(s) 656, 1278, 1420
BseRI GAGGAG 7 cut(s) 42, 45, 48, 51, 54, 57, 1178
BseXI GCAGC 4 cut(s) 260, 734, 1278, 1425
BshNI GGYRCC 1 cut(s) 1206
BsiHKAI GWGCWC 1 cut(s) 1192
BsiHKCI CYCGRG 2 cut(s) 1130, 1160
BslFI GGGAC 4 cut(s) 179, 193, 525, 1246
BslI CCNNNNNNNGG 5 cut(s) 232, 972, 1024, 1069, 1334
BsmAI GTCTC 2 cut(s) 32, 444
BsmFI GGGAC 4 cut(s) 179, 193, 525, 1246
Bso31I GGTCTC 1 cut(s) 444
BsoBI CYCGRG 2 cut(s) 1130, 1160
Bsp1286I GDGCHC 2 cut(s) 750, 1192
Bsp1407I TGTACA 1 cut(s) 621
Bsp143I GATC 5 cut(s) 129, 166, 343, 679, 1306
BspCNI CTCAG 7 cut(s) 477, 574, 687, 762, 784, 877, 1003
BspLI GGNNCC 4 cut(s) 194, 195, 964, 1208
BspMAI CTGCAG 1 cut(s) 724
BspMI ACCTGC 1 cut(s) 958
BspQI GCTCTTC 1 cut(s) 492
BspT107I GGYRCC 1 cut(s) 1206
BspTI CTTAAG 1 cut(s) 985
BspTNI GGTCTC 1 cut(s) 444
BsrDI GCAATG 1 cut(s) 1143
BsrGI TGTACA 1 cut(s) 621
BsrI ACTGG 3 cut(s) 656, 1278, 1420
BssECI CCNNGG 1 cut(s) 966
BssMI GATC 5 cut(s) 129, 166, 343, 679, 1306
BssT1I CCWWGG 1 cut(s) 966
Bst4CI ACNGT 3 cut(s) 186, 394, 1223
Bst6I CTCTTC 4 cut(s) 169, 492, 774, 1176
BstAFI CTTAAG 1 cut(s) 985
BstAPI GCANNNNNTGC 1 cut(s) 1291
BstAUI TGTACA 1 cut(s) 621
BstDEI CTNAG 7 cut(s) 464, 582, 674, 749, 792, 864, 1011
BstEII GGTNACC 1 cut(s) 734
BstENI CCTNNNNNAGG 2 cut(s) 970, 1067
BstF5I GGATG 6 cut(s) 106, 163, 651, 775, 781, 1309
BstHHI GCGC 1 cut(s) 1233
BstKTI GATC 5 cut(s) 132, 169, 346, 682, 1309
BstMAI GTCTC 2 cut(s) 32, 444
BstMBI GATC 5 cut(s) 129, 166, 343, 679, 1306
BstMWI GCNNNNNNNGC 3 cut(s) 483, 1079, 1291
BstPI GGTNACC 1 cut(s) 734
BstSFI CTRYAG 1 cut(s) 720
BstV1I GCAGC 4 cut(s) 260, 734, 1278, 1425
BstV2I GAAGAC 2 cut(s) 197, 760
BstX2I RGATCY 2 cut(s) 343, 679
BstYI RGATCY 2 cut(s) 343, 679
Bsu36I CCTNAGG 2 cut(s) 464, 864
BsuI GTATCC 1 cut(s) 870
BtrI CACGTC 1 cut(s) 613
BtsCI GGATG 6 cut(s) 106, 163, 651, 775, 781, 1309
BtsIMutI CAGTG 2 cut(s) 390, 789
BveI ACCTGC 1 cut(s) 958
CaiI CAGNNNCTG 1 cut(s) 142
CfoI GCGC 1 cut(s) 1233
Cfr13I GGNCC 1 cut(s) 193
Csp6I GTAC 3 cut(s) 622, 853, 1207
CviAII CATG 3 cut(s) 23, 356, 1310
CviQI GTAC 3 cut(s) 622, 853, 1207
DdeI CTNAG 7 cut(s) 464, 582, 674, 749, 792, 864, 1011
DpnI GATC 5 cut(s) 131, 168, 345, 681, 1308
DpnII GATC 5 cut(s) 129, 166, 343, 679, 1306
Eam1104I CTCTTC 4 cut(s) 169, 492, 774, 1176
EarI CTCTTC 4 cut(s) 169, 492, 774, 1176
Eco130I CCWWGG 1 cut(s) 966
Eco24I GRGCYC 1 cut(s) 750
Eco31I GGTCTC 1 cut(s) 444
Eco32I GATATC 1 cut(s) 1374
Eco47I GGWCC 1 cut(s) 193
Eco57I CTGAAG 4 cut(s) 251, 668, 747, 1269
Eco81I CCTNAGG 2 cut(s) 464, 864
Eco88I CYCGRG 2 cut(s) 1130, 1160
Eco91I GGTNACC 1 cut(s) 734
EcoNI CCTNNNNNAGG 2 cut(s) 970, 1067
EcoO109I RGGNCCY 1 cut(s) 193
EcoO65I GGTNACC 1 cut(s) 734
EcoRV GATATC 1 cut(s) 1374
EcoT14I CCWWGG 1 cut(s) 966
EcoT38I GRGCYC 1 cut(s) 750
ErhI CCWWGG 1 cut(s) 966
FaeI CATG 3 cut(s) 26, 359, 1313
FaqI GGGAC 4 cut(s) 179, 193, 525, 1246
FatI CATG 3 cut(s) 22, 355, 1309
FbaI TGATCA 2 cut(s) 129, 1306
Fnu4HI GCNGC 4 cut(s) 249, 723, 1292, 1439
FokI GGATG 6 cut(s) 113, 170, 658, 782, 788, 1316
FriOI GRGCYC 1 cut(s) 750
Fsp4HI GCNGC 4 cut(s) 249, 723, 1292, 1439
FspBI CTAG 4 cut(s) 332, 515, 938, 1197
GlaI GCGC 1 cut(s) 1232
GluI GCNGC 4 cut(s) 249, 723, 1292, 1439
GsuI CTGGAG 3 cut(s) 116, 1295, 1437
HhaI GCGC 1 cut(s) 1233
Hin1II CATG 3 cut(s) 26, 359, 1313
Hin6I GCGC 1 cut(s) 1231
HinP1I GCGC 1 cut(s) 1231
HindIII AAGCTT 3 cut(s) 917, 982, 1054
HinfI GANTC 4 cut(s) 91, 756, 887, 1135
HphI GGTGA 3 cut(s) 47, 888, 1258
Hpy166II GTNNAC 2 cut(s) 388, 1170
Hpy188I TCNGA 8 cut(s) 171, 274, 281, 677, 687, 752, 761, 1012
Hpy8I GTNNAC 2 cut(s) 388, 1170
HpyAV CCTTC 5 cut(s) 226, 230, 836, 1244, 1415
HpyCH4III ACNGT 3 cut(s) 186, 394, 1223
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 9 cut(s) 248, 535, 722, 1082, 1219, 1258, 1285, 1313, 1441
HpyF10VI GCNNNNNNNGC 3 cut(s) 483, 1079, 1291
HpyF3I CTNAG 7 cut(s) 464, 582, 674, 749, 792, 864, 1011
HpySE526I ACGT 1 cut(s) 612
Hsp92II CATG 3 cut(s) 26, 359, 1313
HspAI GCGC 1 cut(s) 1231
KflI GGGWCCC 1 cut(s) 193
KpnI GGTACC 1 cut(s) 1210
Ksp22I TGATCA 2 cut(s) 129, 1306
Kzo9I GATC 5 cut(s) 129, 166, 343, 679, 1306
LguI GCTCTTC 1 cut(s) 492
LmnI GCTCC 2 cut(s) 1187, 1276
Lsp1109I GCAGC 4 cut(s) 260, 734, 1278, 1425
LweI GCATC 2 cut(s) 807, 1069
MaeI CTAG 4 cut(s) 332, 515, 938, 1197
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 4 cut(s) 432, 734, 787, 1242
MalI GATC 5 cut(s) 131, 168, 345, 681, 1308
MboI GATC 5 cut(s) 129, 166, 343, 679, 1306
MflI RGATCY 2 cut(s) 343, 679
MhlI GDGCHC 2 cut(s) 750, 1192
MluCI AATT 4 cut(s) 125, 374, 1353, 1387
MlyI GAGTC 2 cut(s) 750, 896
MroXI GAANNNNTTC 2 cut(s) 501, 1100
MseI TTAA 5 cut(s) 188, 254, 566, 986, 1058
MslI CAYNNNNRTG 1 cut(s) 1224
MspA1I CMGCKG 2 cut(s) 581, 725
MspCI CTTAAG 1 cut(s) 985
MwoI GCNNNNNNNGC 3 cut(s) 483, 1079, 1291
NdeII GATC 5 cut(s) 129, 166, 343, 679, 1306
NlaIII CATG 3 cut(s) 26, 359, 1313
NlaIV GGNNCC 4 cut(s) 194, 195, 964, 1208
NmuCI GTSAC 1 cut(s) 787
OliI CACNNNNGTG 1 cut(s) 1224
PaeR7I CTCGAG 2 cut(s) 1130, 1160
PciSI GCTCTTC 1 cut(s) 492
PdmI GAANNNNTTC 2 cut(s) 501, 1100
PfeI GAWTC 2 cut(s) 91, 1135
PkrI GCNGC 4 cut(s) 250, 724, 1293, 1440
PleI GAGTC 2 cut(s) 750, 895
PpsI GAGTC 2 cut(s) 750, 895
PpuMI RGGWCCY 1 cut(s) 193
Psp5II RGGWCCY 1 cut(s) 193
PspEI GGTNACC 1 cut(s) 734
PspN4I GGNNCC 4 cut(s) 194, 195, 964, 1208
PspPI GGNCC 1 cut(s) 193
PspPPI RGGWCCY 1 cut(s) 193
PstI CTGCAG 1 cut(s) 724
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 2 cut(s) 343, 679
PvuII CAGCTG 2 cut(s) 581, 725
RsaI GTAC 3 cut(s) 623, 854, 1208
RsaNI GTAC 3 cut(s) 622, 853, 1207
RseI CAYNNNNRTG 1 cut(s) 1224
SapI GCTCTTC 1 cut(s) 492
SaqAI TTAA 5 cut(s) 188, 254, 566, 986, 1058
SatI GCNGC 4 cut(s) 249, 723, 1292, 1439
Sau3AI GATC 5 cut(s) 129, 166, 343, 679, 1306
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 2 cut(s) 750, 896
SduI GDGCHC 2 cut(s) 750, 1192
SfaNI GCATC 2 cut(s) 807, 1069
SfcI CTRYAG 1 cut(s) 720
Sfr274I CTCGAG 2 cut(s) 1130, 1160
SinI GGWCC 1 cut(s) 193
SlaI CTCGAG 2 cut(s) 1130, 1160
SmiMI CAYNNNNRTG 1 cut(s) 1224
SmlI CTYRAG 6 cut(s) 404, 926, 985, 1130, 1160, 1201
SmoI CTYRAG 6 cut(s) 404, 926, 985, 1130, 1160, 1201
Sse9I AATT 4 cut(s) 125, 374, 1353, 1387
SspMI CTAG 4 cut(s) 332, 515, 938, 1197
StyI CCWWGG 1 cut(s) 966
TaaI ACNGT 3 cut(s) 186, 394, 1223
TaiI ACGT 1 cut(s) 615
TaqI TCGA 4 cut(s) 148, 810, 1131, 1161
TasI AATT 4 cut(s) 125, 374, 1353, 1387
TatI WGTACW 2 cut(s) 621, 852
TfiI GAWTC 2 cut(s) 91, 1135
Tru1I TTAA 5 cut(s) 188, 254, 566, 986, 1058
Tru9I TTAA 5 cut(s) 188, 254, 566, 986, 1058
TscAI CASTG 2 cut(s) 397, 796
TseFI GTSAC 1 cut(s) 787
TseI GCWGC 4 cut(s) 248, 722, 1291, 1438
Tsp45I GTSAC 1 cut(s) 787
TspDTI ATGAA 5 cut(s) 174, 253, 456, 662, 792
TspRI CASTG 2 cut(s) 397, 796
Vha464I CTTAAG 1 cut(s) 985
VpaK11BI GGWCC 1 cut(s) 193
XagI CCTNNNNNAGG 2 cut(s) 970, 1067
XhoI CTCGAG 2 cut(s) 1130, 1160
XmnI GAANNNNTTC 2 cut(s) 501, 1100
XspI CTAG 4 cut(s) 332, 515, 938, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.