MD14G1174200.v1.1

rpm1,rps3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0019233
Physical Location & Seq
Reverse (-)
26787969 .. 26794662
6694 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1174200.v1.1.491

Sequence Viewer

Length: 1626 bp
ATGGCTAGTTCAAACACCACTGAAGGAGAGACGGAGGAAAGTAGAAACTCTGAAGAGAGCGAAGAAGTTGCGATATCTTGTTCTTCTTCATCTCTTCCACTGGAAACATGCTATGGAACCTTACCGGATTATCTTCAGCACTGCTTAATGTATTGCTCCATCCTCCCTGACTACCATGGGATTCCGAAAGGAAAACTTATTCGGTTACTTGTGGCCCAGGGACTGGTACAAGAAAAAGCAGGGCGGATTATGGAGGATGTGGCAGAAGAAAATTTATATGAATTGATCAGTCTAGGAATGATTCAACTCAAGGAGCATCACTCTGACTCAAGAGGGACAAGATTTCAGGTCTCCTCCCCTGCACGAGAATTCTGTGTTCGTCAAATGGAGGAAGGCAAATTTAAAGTCTTTCGGGTTTTCATTTGCTCAGACATCACACAACTCATATCCCACATGAACAATCATCAAGTTTCTTCTTTGTTCCTTCTTGGGCAAAATCTTTCTCAACATGAAGGTAACTGGTTACAGTTTAATGGTGCAAACTCTTTAAGAGTGCTGGATCTGGAGCGTACCAAAATCAAAACGTTACCAGATGAAGTGGGAGACATGATAAATCTCCTGTATCTTGGCTTGAACCACACTGATATAGAAGAGCTTCCAGAGGGGCTAGGTAAATTAAAAGCTCTACAAACTTTTGATATCAGATGGTGTAGGCATTTAACCGCCTTGCCAAATGATGTACTCTGTCTTGTTAGGCTGAGGCACCTCAAAATGTTCAAGAGCATGTACCAAGATGTTCATGGGATGAAGCTACCTGCCGGAATAGGAAGCTTGAATGACCTTCTGACCCTTACAGGTATACATGCTGGTGGTGGAATTGCAGAAGAATTAGGTAATCTAATACGGCTCAGAAAACTTGGAGTGATGGATGTTGCTGAAGAGAATATCAGTGAGCTACTTGCCTCTATAACGAAGATGTCAGACCTCCTTTCCTTGTCTCTAGAAGGAAAAGACACCTTCAGCGGGGGAAATCCTATGACCTTCTGCGGGGGAAATCTTATCCTCCCGGATTCATTCTCTCCCCCACCATTTCTCCAAAAGCTTCGCTTGGAAGGCGTTGGAGGAAAGCTACCTACTTGGTTCGGTTCCTTGGAAAGGCTTACAAATCTAAGATTAGGAAACTCTCATATATCTGAGGACTCATTATTGGTACTTCAACTGCTACCCAACTTGGAAAATCTTAGCCTTTGGAATGCTTATGATGCTAAGAAAATAGGGAAAGAATTCTGTTGCATTGGTGGATTTCCAAAGCTCCAGGTTCTAACCATTGCTTCGCGTGTTCTTGAGAAGTGGATAGAAATTGAAGAGGGAGCACTACTGAGCTTGAAATATCTCCACTTGCGCAATTGTTTGCATCTGCGGATGCTTCCTGAAGGTCTGCAGTTTCTCACCAACCTTGAGCTGTTGGATTTGTTGCCTTTGCCGGATGATCTTGCAGAACGGCTGAAACCTGATGGCGGAGAGGAAAACTACAAAATCAGGCACATTCCAGAGGTAACAATTATGACTAACTCGATGGTGAAAGAATATGCTCAAGCCAAGAGAGAGGGGAGCCAATGTGAGTGA

Protein Analysis

542

Amino Acids

60.58

Weight (kDa)

5.44

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 54 - 117 1.3e-09 Disease resistance protein Winged helix domain
LRR_14 PF23598 175 - 444 3.9e-37 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06010 FvH4_5g32740
malus_domestica MD14G1174200.v1.1
prunus_persica Prupe.5G167700_v2.0.a1
rosa_multiflora Rmu_sc0002040.1_g000041 Rmu_sc0028138.1_g000001 Rmu_sc0034887.1_g000001
rosa_rugosa Rorug07G0005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1403
Acc36I ACCTGC 1 cut(s) 823
AccB1I GGYRCC 1 cut(s) 762
AccB7I CCANNNNNTGG 1 cut(s) 223
AccI GTMKAC 1 cut(s) 859
AccII CGCG 1 cut(s) 1336
AciI CCGC 6 cut(s) 244, 723, 1023, 1047, 1420, 1518
AclI AACGTT 1 cut(s) 584
AclWI GGATC 1 cut(s) 567
AcsI RAATTY 4 cut(s) 271, 368, 398, 1283
AcuI CTGAAG 6 cut(s) 42, 72, 119, 957, 1003, 1452
AfaI GTAC 5 cut(s) 228, 571, 741, 788, 1212
AfiI CCNNNNNNNGG 5 cut(s) 223, 1023, 1047, 1155, 1517
AgsI TTSAA 8 cut(s) 12, 305, 634, 778, 835, 1217, 1364, 1387
AjnI CCWGG 2 cut(s) 216, 1314
Alw21I GWGCWC 1 cut(s) 1375
Alw26I GTCTC 4 cut(s) 23, 355, 597, 1002
AlwI GGATC 1 cut(s) 567
AlwNI CAGNNNCTG 1 cut(s) 223
AoxI GGCC 1 cut(s) 213
ApoI RAATTY 4 cut(s) 271, 368, 398, 1283
ArsI GACNNNNNNTTYG 2 cut(s) 390, 422
Asp700I GAANNNNTTC 2 cut(s) 654, 1283
AspLEI GCGC 1 cut(s) 1404
AspS9I GGNCC 1 cut(s) 214
AsuC2I CCSGG 1 cut(s) 1067
AsuHPI GGTGA 2 cut(s) 1441, 1591
BanI GGYRCC 1 cut(s) 762
BauI CACGAG 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 1375
BbvCI CCTCAGC 1 cut(s) 758
BccI CCATC 5 cut(s) 167, 699, 919, 1508, 1570
BceAI ACGGC 2 cut(s) 920, 1517
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BciT130I CCWGG 2 cut(s) 218, 1316
BclI TGATCA 1 cut(s) 285
BcnI CCSGG 1 cut(s) 1067
BcoDI GTCTC 4 cut(s) 23, 355, 597, 1002
BfaI CTAG 4 cut(s) 6, 293, 668, 1001
BfmI CTRYAG 1 cut(s) 1439
BfuAI ACCTGC 1 cut(s) 823
Bme1390I CCNGG 3 cut(s) 218, 1067, 1316
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 4 cut(s) 118, 764, 1147, 1613
BmrFI CCNGG 3 cut(s) 218, 1067, 1316
BmsI GCATC 4 cut(s) 325, 1252, 1413, 1423
BplI GAGNNNNNCTC 2 cut(s) 305, 337
BpmI CTGGAG 2 cut(s) 584, 1298
Bpu10I CCTNAGC 1 cut(s) 758
BpuEI CTTGAG 5 cut(s) 293, 313, 1364, 1478, 1578
BpuMI CCSGG 1 cut(s) 1067
BsaI GGTCTC 1 cut(s) 355
BsaJI CCNNGG 4 cut(s) 175, 216, 217, 1149
BsaWI WCCGGW 1 cut(s) 124
BsaXI ACNNNNNCTCC 3 cut(s) 1077, 1107, 1603
Bsc4I CCNNNNNNNGG 5 cut(s) 223, 1023, 1047, 1155, 1517
Bse1I ACTGG 3 cut(s) 105, 228, 524
Bse3DI GCAATG 1 cut(s) 1326
BseBI CCWGG 2 cut(s) 218, 1316
BseDI CCNNGG 4 cut(s) 175, 216, 217, 1149
BseGI GGATG 6 cut(s) 159, 262, 810, 934, 1428, 1492
BseLI CCNNNNNNNGG 5 cut(s) 223, 1023, 1047, 1155, 1517
BseMI GCAATG 1 cut(s) 1326
BseMII CTCAG 5 cut(s) 441, 749, 922, 1185, 1370
BseNI ACTGG 3 cut(s) 105, 228, 524
BseRI GAGGAG 1 cut(s) 343
BsgI GTGCAG 1 cut(s) 345
Bsh1236I CGCG 1 cut(s) 1336
BshFI GGCC 1 cut(s) 215
BshNI GGYRCC 1 cut(s) 762
BsiHKAI GWGCWC 1 cut(s) 1375
BsiSI CCGG 4 cut(s) 125, 819, 1067, 1484
BslFI GGGAC 2 cut(s) 234, 349
BslI CCNNNNNNNGG 5 cut(s) 223, 1023, 1047, 1155, 1517
BsmAI GTCTC 4 cut(s) 23, 355, 597, 1002
BsmBI CGTCTC 1 cut(s) 23
BsmFI GGGAC 2 cut(s) 234, 349
BsmI GAATGC 1 cut(s) 1258
BsnI GGCC 1 cut(s) 215
Bso31I GGTCTC 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 1375
Bsp143I GATC 3 cut(s) 285, 559, 1489
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 6 cut(s) 244, 723, 1023, 1047, 1420, 1518
BspANI GGCC 1 cut(s) 215
BspCNI CTCAG 5 cut(s) 440, 750, 921, 1186, 1371
BspFNI CGCG 1 cut(s) 1336
BspLI GGNNCC 4 cut(s) 118, 764, 1147, 1613
BspMAI CTGCAG 1 cut(s) 1443
BspMI ACCTGC 1 cut(s) 823
BspPI GGATC 1 cut(s) 567
BspQI GCTCTTC 1 cut(s) 645
BspT107I GGYRCC 1 cut(s) 762
BspTNI GGTCTC 1 cut(s) 355
BsrDI GCAATG 1 cut(s) 1326
BsrI ACTGG 3 cut(s) 105, 228, 524
BssECI CCNNGG 4 cut(s) 175, 216, 217, 1149
BssMI GATC 3 cut(s) 285, 559, 1489
BssNAI GTATAC 1 cut(s) 860
BssSI CACGAG 1 cut(s) 363
BssT1I CCWWGG 2 cut(s) 175, 1149
Bst1107I GTATAC 1 cut(s) 860
Bst2BI CACGAG 1 cut(s) 363
Bst2UI CCWGG 2 cut(s) 218, 1316
Bst4CI ACNGT 1 cut(s) 528
Bst6I CTCTTC 5 cut(s) 48, 99, 645, 933, 1359
BstDEI CTNAG 8 cut(s) 427, 758, 908, 1169, 1194, 1241, 1266, 1379
BstDSI CCRYGG 1 cut(s) 175
BstENI CCTNNNNNAGG 1 cut(s) 1153
BstF5I GGATG 6 cut(s) 159, 262, 810, 934, 1428, 1492
BstFNI CGCG 1 cut(s) 1336
BstHHI GCGC 1 cut(s) 1404
BstKTI GATC 3 cut(s) 288, 562, 1492
BstMAI GTCTC 4 cut(s) 23, 355, 597, 1002
BstMBI GATC 3 cut(s) 285, 559, 1489
BstMWI GCNNNNNNNGC 2 cut(s) 1113, 1262
BstNI CCWGG 2 cut(s) 218, 1316
BstNSI RCATGY 3 cut(s) 111, 787, 866
BstSCI CCNGG 3 cut(s) 216, 1065, 1314
BstSFI CTRYAG 1 cut(s) 1439
BstUI CGCG 1 cut(s) 1336
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
BstZ17I GTATAC 1 cut(s) 860
BsuRI GGCC 1 cut(s) 215
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 6 cut(s) 159, 262, 810, 934, 1428, 1492
BtsI GCAGTG 1 cut(s) 139
BtsIMutI CAGTG 5 cut(s) 18, 98, 139, 639, 955
BveI ACCTGC 1 cut(s) 823
CaiI CAGNNNCTG 1 cut(s) 223
CfoI GCGC 1 cut(s) 1404
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 5 cut(s) 227, 570, 740, 787, 1211
CviAII CATG 8 cut(s) 108, 176, 454, 509, 607, 784, 800, 863
CviQI GTAC 5 cut(s) 227, 570, 740, 787, 1211
DdeI CTNAG 8 cut(s) 427, 758, 908, 1169, 1194, 1241, 1266, 1379
DpnI GATC 3 cut(s) 287, 561, 1491
DpnII GATC 3 cut(s) 285, 559, 1489
DraI TTTAAA 1 cut(s) 403
Eam1104I CTCTTC 5 cut(s) 48, 99, 645, 933, 1359
EarI CTCTTC 5 cut(s) 48, 99, 645, 933, 1359
EciI GGCGGA 2 cut(s) 259, 1533
Eco130I CCWWGG 2 cut(s) 175, 1149
Eco31I GGTCTC 1 cut(s) 355
Eco32I GATATC 2 cut(s) 75, 700
Eco57I CTGAAG 6 cut(s) 42, 72, 119, 957, 1003, 1452
EcoNI CCTNNNNNAGG 1 cut(s) 1153
EcoRI GAATTC 2 cut(s) 368, 1283
EcoRII CCWGG 2 cut(s) 216, 1314
EcoRV GATATC 2 cut(s) 75, 700
EcoT14I CCWWGG 2 cut(s) 175, 1149
ErhI CCWWGG 2 cut(s) 175, 1149
Esp3I CGTCTC 1 cut(s) 23
FaeI CATG 8 cut(s) 111, 179, 457, 512, 610, 787, 803, 866
FalI AAGNNNNNCTT 4 cut(s) 180, 212, 1091, 1123
FaqI GGGAC 2 cut(s) 234, 349
FatI CATG 8 cut(s) 107, 175, 453, 508, 606, 783, 799, 862
FauI CCCGC 2 cut(s) 1016, 1040
FbaI TGATCA 1 cut(s) 285
FblI GTMKAC 1 cut(s) 859
FokI GGATG 6 cut(s) 146, 269, 817, 941, 1435, 1499
FspBI CTAG 4 cut(s) 6, 293, 668, 1001
FspI TGCGCA 1 cut(s) 1403
GlaI GCGC 1 cut(s) 1403
GsuI CTGGAG 2 cut(s) 584, 1298
HaeIII GGCC 1 cut(s) 215
HapII CCGG 4 cut(s) 125, 819, 1067, 1484
HhaI GCGC 1 cut(s) 1404
Hin1II CATG 8 cut(s) 111, 179, 457, 512, 610, 787, 803, 866
Hin6I GCGC 1 cut(s) 1402
HinP1I GCGC 1 cut(s) 1402
HindIII AAGCTT 2 cut(s) 829, 1100
HinfI GANTC 5 cut(s) 181, 301, 326, 1070, 1199
HpaII CCGG 4 cut(s) 125, 819, 1067, 1484
HphI GGTGA 2 cut(s) 1441, 1591
Hpy166II GTNNAC 1 cut(s) 860
Hpy188I TCNGA 9 cut(s) 52, 186, 325, 430, 704, 846, 911, 982, 1195
Hpy188III TCNNGA 8 cut(s) 330, 563, 659, 778, 1001, 1343, 1430, 1550
Hpy8I GTNNAC 1 cut(s) 860
HpyCH4III ACNGT 1 cut(s) 528
HpyCH4IV ACGT 1 cut(s) 584
HpyCH4V TGCA 7 cut(s) 362, 539, 881, 1293, 1414, 1441, 1496
HpyF10VI GCNNNNNNNGC 2 cut(s) 1113, 1262
HpyF3I CTNAG 8 cut(s) 427, 758, 908, 1169, 1194, 1241, 1266, 1379
HpySE526I ACGT 1 cut(s) 584
Hsp92II CATG 8 cut(s) 111, 179, 457, 512, 610, 787, 803, 866
HspAI GCGC 1 cut(s) 1402
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 3 cut(s) 285, 559, 1489
LguI GCTCTTC 1 cut(s) 645
LmnI GCTCC 6 cut(s) 161, 313, 565, 1317, 1370, 1611
LweI GCATC 4 cut(s) 325, 1252, 1413, 1423
MaeI CTAG 4 cut(s) 6, 293, 668, 1001
MaeII ACGT 1 cut(s) 584
MaeIII GTNAC 5 cut(s) 204, 515, 522, 585, 1555
MalI GATC 3 cut(s) 287, 561, 1491
MboI GATC 3 cut(s) 285, 559, 1489
MfeI CAATTG 1 cut(s) 1405
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 1 cut(s) 1375
MlyI GAGTC 2 cut(s) 320, 1193
MmeI TCCRAC 2 cut(s) 1099, 1446
MroXI GAANNNNTTC 2 cut(s) 654, 1283
MseI TTAA 6 cut(s) 146, 402, 531, 548, 677, 719
MslI CAYNNNNRTG 1 cut(s) 867
MspA1I CMGCKG 1 cut(s) 1023
MspI CCGG 4 cut(s) 125, 819, 1067, 1484
MspR9I CCNGG 3 cut(s) 218, 1067, 1316
MunI CAATTG 1 cut(s) 1405
Mva1269I GAATGC 1 cut(s) 1258
MvaI CCWGG 2 cut(s) 218, 1316
MvnI CGCG 1 cut(s) 1336
MwoI GCNNNNNNNGC 2 cut(s) 1113, 1262
NciI CCSGG 1 cut(s) 1067
NcoI CCATGG 1 cut(s) 175
NdeII GATC 3 cut(s) 285, 559, 1489
NlaIII CATG 8 cut(s) 111, 179, 457, 512, 610, 787, 803, 866
NlaIV GGNNCC 4 cut(s) 118, 764, 1147, 1613
NsbI TGCGCA 1 cut(s) 1403
NspI RCATGY 3 cut(s) 111, 787, 866
PasI CCCWGGG 1 cut(s) 217
PciSI GCTCTTC 1 cut(s) 645
PctI GAATGC 1 cut(s) 1258
PdmI GAANNNNTTC 2 cut(s) 654, 1283
PfeI GAWTC 3 cut(s) 181, 301, 1070
PflMI CCANNNNNTGG 1 cut(s) 223
PfoI TCCNGGA 1 cut(s) 1065
PleI GAGTC 2 cut(s) 320, 1193
PpsI GAGTC 2 cut(s) 320, 1193
Psp1406I AACGTT 1 cut(s) 584
Psp6I CCWGG 2 cut(s) 216, 1314
PspGI CCWGG 2 cut(s) 216, 1314
PspN4I GGNNCC 4 cut(s) 118, 764, 1147, 1613
PspPI GGNCC 1 cut(s) 214
PstI CTGCAG 1 cut(s) 1443
PstNI CAGNNNCTG 1 cut(s) 223
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 5 cut(s) 228, 571, 741, 788, 1212
RsaNI GTAC 5 cut(s) 227, 570, 740, 787, 1211
RseI CAYNNNNRTG 1 cut(s) 867
SapI GCTCTTC 1 cut(s) 645
SaqAI TTAA 6 cut(s) 146, 402, 531, 548, 677, 719
Sau3AI GATC 3 cut(s) 285, 559, 1489
Sau96I GGNCC 1 cut(s) 214
SchI GAGTC 2 cut(s) 320, 1193
ScrFI CCNGG 3 cut(s) 218, 1067, 1316
SduI GDGCHC 1 cut(s) 1375
SfaNI GCATC 4 cut(s) 325, 1252, 1413, 1423
SfcI CTRYAG 1 cut(s) 1439
SmiMI CAYNNNNRTG 1 cut(s) 867
SmlI CTYRAG 5 cut(s) 308, 328, 1343, 1457, 1593
SmoI CTYRAG 5 cut(s) 308, 328, 1343, 1457, 1593
SsiI CCGC 6 cut(s) 244, 723, 1023, 1047, 1420, 1518
SspMI CTAG 4 cut(s) 6, 293, 668, 1001
StyD4I CCNGG 3 cut(s) 216, 1065, 1314
StyI CCWWGG 2 cut(s) 175, 1149
TaaI ACNGT 1 cut(s) 528
TaiI ACGT 1 cut(s) 587
TaqI TCGA 1 cut(s) 1574
TatI WGTACW 1 cut(s) 739
TfiI GAWTC 3 cut(s) 181, 301, 1070
Tru1I TTAA 6 cut(s) 146, 402, 531, 548, 677, 719
Tru9I TTAA 6 cut(s) 146, 402, 531, 548, 677, 719
TscAI CASTG 5 cut(s) 25, 105, 146, 646, 955
TspDTI ATGAA 9 cut(s) 78, 294, 409, 470, 525, 609, 788, 821, 1062
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 5 cut(s) 25, 105, 146, 646, 955
Van91I CCANNNNNTGG 1 cut(s) 223
XagI CCTNNNNNAGG 1 cut(s) 1153
XapI RAATTY 4 cut(s) 271, 368, 398, 1283
XbaI TCTAGA 1 cut(s) 1000
XceI RCATGY 3 cut(s) 111, 787, 866
XcmI CCANNNNNNNNNTGG 1 cut(s) 797
XmiI GTMKAC 1 cut(s) 859
XmnI GAANNNNTTC 2 cut(s) 654, 1283
XspI CTAG 4 cut(s) 6, 293, 668, 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.