Rmu_sc0002040.1_g000041

rpm1,rps3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002040.1
OG0019233
Physical Location & Seq
Reverse (-)
163259 .. 164410
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002040.1_g000041.1.cds

Sequence Viewer

Length: 1152 bp
atgaacaatcttcaactaactgctttgttcctgctagcaaatcaaaatatatctcaacatgatggtagttggctgcaatttgatggggcgaacactttaagagtgcttgagctacaagataccaaaatcaaaaagttaccagatgaagtgggaagcctaatacacctcacgtttcttggcttgaaaagcacggatataaatgaacttccagaggggctaggcagattagaagccctgcaaactttggatattagatggtgtggggatttaacaggcttgtcagctgagataataaatcttgtaaggttgaggcacctcaagatgttcaagaacaaatgcatttctgggatgaagctaccggcaggaataggaagactcacagatcttctgaccctgacaggtatacacgctggtggtggcactgcagctgaagtaggtaacctgacagggctcagaagactaggagtgatggatgtggacgaggagaatgtcattgagctatttgcctccattatgaagatgcaaggccttctttccttgtgtttagaagcaaaacacacattccctcagggacaacttattctcctggagtccttctcacctccaccatttcttcaaaagcttcacctcaaaggcatcctagaaaagccgcctgcttggtttggttccttggaaaggctcacaaacttaagattaggcttctcccatttatctgagaacccatcattggtacttcaagtcctacccaacttgaaaaacttaaccctttggcaggcttatgatgcaaagaaaatagggaaagagttctgtagtactggtgggttttccaagctcgaggttctcatcattgcttctcgtgttcttgaggatttgacagaacttgaagagggagcactacctagcttgaagtacctccacttgcacagttgtctgcggttgaggatgttacctgaaggtctgcaatccctcaccacactgaagcagttgcatttgctgcctctgctggatgatcatgcagaacggctgaaacctgatggtggagaggagtattacaaaattaagcacattccagagatatcatacattaccaactctatggtacaagaatatgtgaaaactgggcatggaggaatagtcatgatgcaaggataa

Protein Analysis

383

Amino Acids

42.57

Weight (kDa)

6.36

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019233)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06010 FvH4_5g32740
malus_domestica MD14G1174200.v1.1
prunus_persica Prupe.5G167700_v2.0.a1
rosa_multiflora Rmu_sc0002040.1_g000041 Rmu_sc0028138.1_g000001 Rmu_sc0034887.1_g000001
rosa_rugosa Rorug07G0005200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 403
AciI CCGC 2 cut(s) 650, 934
AcuI CTGAAG 3 cut(s) 450, 972, 998
AfaI GTAC 4 cut(s) 732, 814, 911, 1101
AfiI CCNNNNNNNGG 4 cut(s) 675, 727, 772, 1037
AflII CTTAAG 1 cut(s) 688
AgsI TTSAA 8 cut(s) 14, 184, 328, 617, 737, 754, 884, 907
AjnI CCWGG 1 cut(s) 585
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AleI CACNNNNGTG 1 cut(s) 411
AluBI AGCT 8 cut(s) 112, 284, 355, 428, 499, 622, 832, 903
AluI AGCT 8 cut(s) 112, 284, 355, 428, 499, 622, 832, 903
Alw21I GWGCWC 1 cut(s) 895
Ama87I CYCGRG 1 cut(s) 833
AoxI GGCC 1 cut(s) 526
ApeKI GCWGC 3 cut(s) 73, 425, 994
Asp700I GAANNNNTTC 1 cut(s) 803
AsuHPI GGTGA 3 cut(s) 591, 617, 961
AsuNHI GCTAGC 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 833
AxyI CCTNAGG 1 cut(s) 567
BanI GGYRCC 1 cut(s) 312
BanII GRGCYC 1 cut(s) 453
BauI CACGAG 1 cut(s) 855
BbsI GAAGAC 2 cut(s) 379, 463
Bbv12I GWGCWC 1 cut(s) 895
BbvI GCAGC 3 cut(s) 60, 437, 981
BccI CCATC 6 cut(s) 56, 77, 249, 463, 730, 1028
BceAI ACGGC 1 cut(s) 1037
BciT130I CCWGG 1 cut(s) 587
BclI TGATCA 1 cut(s) 1009
BfaI CTAG 5 cut(s) 35, 218, 461, 641, 900
BfmI CTRYAG 2 cut(s) 423, 808
BfrI CTTAAG 1 cut(s) 688
BglII AGATCT 1 cut(s) 382
BisI GCNGC 4 cut(s) 74, 426, 650, 995
BlsI GCNGC 4 cut(s) 75, 427, 651, 996
BmcAI AGTACT 1 cut(s) 814
Bme1390I CCNGG 1 cut(s) 587
BmeT110I CYCGRG 1 cut(s) 833
BmiI GGNNCC 2 cut(s) 314, 667
BmrFI CCNGG 1 cut(s) 587
BmrI ACTGGG 1 cut(s) 1128
BmsI GCATC 4 cut(s) 510, 645, 772, 1131
BmtI GCTAGC 1 cut(s) 38
BmuI ACTGGG 1 cut(s) 1128
BpiI GAAGAC 2 cut(s) 379, 463
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 1 cut(s) 608
BpuEI CTTGAG 3 cut(s) 128, 302, 884
BsaJI CCNNGG 1 cut(s) 669
BsaXI ACNNNNNCTCC 4 cut(s) 567, 597, 1119, 1149
Bsc4I CCNNNNNNNGG 4 cut(s) 675, 727, 772, 1037
Bse118I RCCGGY 1 cut(s) 358
Bse1I ACTGG 2 cut(s) 820, 1123
Bse21I CCTNAGG 1 cut(s) 567
Bse3DI GCAATG 1 cut(s) 846
BseBI CCWGG 1 cut(s) 587
BseDI CCNNGG 1 cut(s) 669
BseGI GGATG 5 cut(s) 354, 478, 636, 948, 1012
BseLI CCNNNNNNNGG 4 cut(s) 675, 727, 772, 1037
BseMI GCAATG 1 cut(s) 846
BseMII CTCAG 4 cut(s) 276, 466, 581, 705
BseNI ACTGG 2 cut(s) 820, 1123
BseRI GAGGAG 2 cut(s) 497, 1058
BseXI GCAGC 3 cut(s) 60, 437, 981
BshFI GGCC 1 cut(s) 528
BshNI GGYRCC 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 895
BsiHKCI CYCGRG 1 cut(s) 833
BsiSI CCGG 1 cut(s) 359
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 4 cut(s) 675, 727, 772, 1037
BsmFI GGGAC 1 cut(s) 585
BsnI GGCC 1 cut(s) 528
BsoBI CYCGRG 1 cut(s) 833
Bsp1286I GDGCHC 2 cut(s) 453, 895
Bsp143I GATC 2 cut(s) 382, 1009
BspACI CCGC 2 cut(s) 650, 934
BspANI GGCC 1 cut(s) 528
BspCNI CTCAG 4 cut(s) 277, 465, 580, 706
BspHI TCATGA 1 cut(s) 1137
BspLI GGNNCC 2 cut(s) 314, 667
BspMAI CTGCAG 1 cut(s) 427
BspOI GCTAGC 1 cut(s) 38
BspT107I GGYRCC 1 cut(s) 312
BspTI CTTAAG 1 cut(s) 688
BsrDI GCAATG 1 cut(s) 846
BsrFI RCCGGY 1 cut(s) 358
BsrI ACTGG 2 cut(s) 820, 1123
BssAI RCCGGY 1 cut(s) 358
BssECI CCNNGG 1 cut(s) 669
BssMI GATC 2 cut(s) 382, 1009
BssNAI GTATAC 1 cut(s) 404
BssSI CACGAG 1 cut(s) 855
BssT1I CCWWGG 1 cut(s) 669
Bst1107I GTATAC 1 cut(s) 404
Bst2BI CACGAG 1 cut(s) 855
Bst2UI CCWGG 1 cut(s) 587
Bst4CI ACNGT 1 cut(s) 926
Bst6I CTCTTC 1 cut(s) 879
BstAFI CTTAAG 1 cut(s) 688
BstAPI GCANNNNNTGC 1 cut(s) 994
BstC8I GCNNGC 3 cut(s) 36, 654, 774
BstDEI CTNAG 4 cut(s) 285, 452, 567, 714
BstEII GGTNACC 1 cut(s) 437
BstENI CCTNNNNNAGG 2 cut(s) 673, 770
BstF5I GGATG 5 cut(s) 354, 478, 636, 948, 1012
BstKTI GATC 2 cut(s) 385, 1012
BstMBI GATC 2 cut(s) 382, 1009
BstMWI GCNNNNNNNGC 4 cut(s) 186, 782, 994, 1000
BstNI CCWGG 1 cut(s) 587
BstPI GGTNACC 1 cut(s) 437
BstSCI CCNGG 1 cut(s) 585
BstSFI CTRYAG 2 cut(s) 423, 808
BstV1I GCAGC 3 cut(s) 60, 437, 981
BstV2I GAAGAC 2 cut(s) 379, 463
BstX2I RGATCY 1 cut(s) 382
BstXI CCANNNNNNTGG 1 cut(s) 1096
BstYI RGATCY 1 cut(s) 382
BstZ17I GTATAC 1 cut(s) 404
Bsu36I CCTNAGG 1 cut(s) 567
BsuRI GGCC 1 cut(s) 528
BtsCI GGATG 5 cut(s) 354, 478, 636, 948, 1012
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 2 cut(s) 420, 974
Cac8I GCNNGC 3 cut(s) 36, 654, 774
CciI TCATGA 1 cut(s) 1137
Cfr10I RCCGGY 1 cut(s) 358
Csp6I GTAC 4 cut(s) 731, 813, 910, 1100
CviAII CATG 4 cut(s) 59, 1013, 1124, 1138
CviQI GTAC 4 cut(s) 731, 813, 910, 1100
DdeI CTNAG 4 cut(s) 285, 452, 567, 714
DpnI GATC 2 cut(s) 384, 1011
DpnII GATC 2 cut(s) 382, 1009
Eam1104I CTCTTC 1 cut(s) 879
EarI CTCTTC 1 cut(s) 879
Eco130I CCWWGG 1 cut(s) 669
Eco147I AGGCCT 1 cut(s) 528
Eco24I GRGCYC 1 cut(s) 453
Eco32I GATATC 1 cut(s) 1077
Eco57I CTGAAG 3 cut(s) 450, 972, 998
Eco81I CCTNAGG 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 833
Eco91I GGTNACC 1 cut(s) 437
EcoNI CCTNNNNNAGG 2 cut(s) 673, 770
EcoO65I GGTNACC 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 585
EcoRV GATATC 1 cut(s) 1077
EcoT14I CCWWGG 1 cut(s) 669
EcoT22I ATGCAT 1 cut(s) 341
EcoT38I GRGCYC 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 669
FaeI CATG 4 cut(s) 62, 1016, 1127, 1141
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 1 cut(s) 585
FatI CATG 4 cut(s) 58, 1012, 1123, 1137
FbaI TGATCA 1 cut(s) 1009
FblI GTMKAC 1 cut(s) 403
Fnu4HI GCNGC 4 cut(s) 74, 426, 650, 995
FokI GGATG 5 cut(s) 361, 485, 623, 955, 1019
FriOI GRGCYC 1 cut(s) 453
Fsp4HI GCNGC 4 cut(s) 74, 426, 650, 995
FspBI CTAG 5 cut(s) 35, 218, 461, 641, 900
GluI GCNGC 4 cut(s) 74, 426, 650, 995
GsuI CTGGAG 1 cut(s) 608
HaeIII GGCC 1 cut(s) 528
HapII CCGG 1 cut(s) 359
Hin1II CATG 4 cut(s) 62, 1016, 1127, 1141
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 2 cut(s) 375, 590
HpaII CCGG 1 cut(s) 359
HphI GGTGA 3 cut(s) 591, 617, 961
Hpy166II GTNNAC 2 cut(s) 404, 478
Hpy188I TCNGA 3 cut(s) 390, 455, 715
Hpy188III TCNNGA 6 cut(s) 209, 319, 328, 863, 1070, 1138
Hpy8I GTNNAC 2 cut(s) 404, 478
HpyAV CCTTC 3 cut(s) 539, 604, 947
HpyCH4III ACNGT 1 cut(s) 926
HpyCH4IV ACGT 1 cut(s) 170
HpyF10VI GCNNNNNNNGC 4 cut(s) 186, 782, 994, 1000
HpyF3I CTNAG 4 cut(s) 285, 452, 567, 714
HpySE526I ACGT 1 cut(s) 170
Hsp92II CATG 4 cut(s) 62, 1016, 1127, 1141
Ksp22I TGATCA 1 cut(s) 1009
Kzo9I GATC 2 cut(s) 382, 1009
LmnI GCTCC 1 cut(s) 890
Lsp1109I GCAGC 3 cut(s) 60, 437, 981
LweI GCATC 4 cut(s) 510, 645, 772, 1131
MaeI CTAG 5 cut(s) 35, 218, 461, 641, 900
MaeII ACGT 1 cut(s) 170
MaeIII GTNAC 3 cut(s) 135, 437, 945
MalI GATC 2 cut(s) 384, 1011
MboI GATC 2 cut(s) 382, 1009
MboII GAAGA 6 cut(s) 377, 384, 468, 529, 605, 896
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 453, 895
MluCI AATT 2 cut(s) 77, 1056
MlyI GAGTC 2 cut(s) 369, 599
Mph1103I ATGCAT 1 cut(s) 341
MroXI GAANNNNTTC 1 cut(s) 803
MseI TTAA 5 cut(s) 98, 269, 689, 761, 1059
MslI CAYNNNNRTG 1 cut(s) 411
MspA1I CMGCKG 2 cut(s) 284, 428
MspCI CTTAAG 1 cut(s) 688
MspI CCGG 1 cut(s) 359
MspR9I CCNGG 1 cut(s) 587
MvaI CCWGG 1 cut(s) 587
MwoI GCNNNNNNNGC 4 cut(s) 186, 782, 994, 1000
NdeII GATC 2 cut(s) 382, 1009
NheI GCTAGC 1 cut(s) 34
NlaIII CATG 4 cut(s) 62, 1016, 1127, 1141
NlaIV GGNNCC 2 cut(s) 314, 667
NsiI ATGCAT 1 cut(s) 341
OliI CACNNNNGTG 1 cut(s) 411
PaeR7I CTCGAG 1 cut(s) 833
PagI TCATGA 1 cut(s) 1137
PceI AGGCCT 1 cut(s) 528
PdmI GAANNNNTTC 1 cut(s) 803
PfoI TCCNGGA 1 cut(s) 585
PkrI GCNGC 4 cut(s) 75, 427, 651, 996
PleI GAGTC 2 cut(s) 369, 598
PpsI GAGTC 2 cut(s) 369, 598
Psp6I CCWGG 1 cut(s) 585
PspEI GGTNACC 1 cut(s) 437
PspGI CCWGG 1 cut(s) 585
PspN4I GGNNCC 2 cut(s) 314, 667
PspXI VCTCGAGB 1 cut(s) 833
PstI CTGCAG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 382
PvuII CAGCTG 2 cut(s) 284, 428
RsaI GTAC 4 cut(s) 732, 814, 911, 1101
RsaNI GTAC 4 cut(s) 731, 813, 910, 1100
RseI CAYNNNNRTG 1 cut(s) 411
SaqAI TTAA 5 cut(s) 98, 269, 689, 761, 1059
SatI GCNGC 4 cut(s) 74, 426, 650, 995
Sau3AI GATC 2 cut(s) 382, 1009
ScaI AGTACT 1 cut(s) 814
SchI GAGTC 2 cut(s) 369, 599
ScrFI CCNGG 1 cut(s) 587
SduI GDGCHC 2 cut(s) 453, 895
SfaNI GCATC 4 cut(s) 510, 645, 772, 1131
SfcI CTRYAG 2 cut(s) 423, 808
Sfr274I CTCGAG 1 cut(s) 833
SlaI CTCGAG 1 cut(s) 833
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 5 cut(s) 107, 317, 688, 833, 863
SmoI CTYRAG 5 cut(s) 107, 317, 688, 833, 863
Sse9I AATT 2 cut(s) 77, 1056
SseBI AGGCCT 1 cut(s) 528
SsiI CCGC 2 cut(s) 650, 934
SspMI CTAG 5 cut(s) 35, 218, 461, 641, 900
StuI AGGCCT 1 cut(s) 528
StyD4I CCNGG 1 cut(s) 585
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 1 cut(s) 926
TaiI ACGT 1 cut(s) 173
TaqI TCGA 1 cut(s) 834
TasI AATT 2 cut(s) 77, 1056
TatI WGTACW 1 cut(s) 812
TauI GCSGC 1 cut(s) 652
Tru1I TTAA 5 cut(s) 98, 269, 689, 761, 1059
Tru9I TTAA 5 cut(s) 98, 269, 689, 761, 1059
TscAI CASTG 2 cut(s) 427, 981
TseI GCWGC 3 cut(s) 73, 425, 994
TspDTI ATGAA 5 cut(s) 17, 159, 216, 365, 530
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 2 cut(s) 427, 981
Vha464I CTTAAG 1 cut(s) 688
XagI CCTNNNNNAGG 2 cut(s) 673, 770
XhoI CTCGAG 1 cut(s) 833
XmiI GTMKAC 1 cut(s) 403
XmnI GAANNNNTTC 1 cut(s) 803
XspI CTAG 5 cut(s) 35, 218, 461, 641, 900
ZrmI AGTACT 1 cut(s) 814
Zsp2I ATGCAT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.