FvH4_5g07610

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014738
Physical Location & Seq
Reverse (-)
4431637 .. 4433238
1602 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07610.t1

Sequence Viewer

Length: 1602 bp
ATGGCTCCTGGCCATGGCCGACGTTTCATGACGGAGCACGAGTGCACTGATCATGAAATTAGGAGTAATCGAATGAGTGACCTCGAACTCCATGGTCTGAATCATTCATCTATGGCTTGCTATTCTTCATATCCTTACATGGCATCACTTGACCAGGAGGGCAGTGCAAATGATTCTTCTTCCTTGATCAATCCCTATTCGGTTCATGAAAGCCGGGACCGTAAGAGGCTGAAAAGGACTATAAGTATCGATGACTCACTGTCGAGTATTACCAGTATCAACGGCGGCATGATGAGCCGCAGCAGCAGTACTAATAGCTTGACCACCTTGCCAAGGCTTCATTTCCGGGATCATATATGGACTTACACACAACGGTACCTTGCAGCCGAAGCTGTTGAGGAGGCTGCAGCGGCTATGATCAATGCCGAGGAAAATGGAGCTGAACAAGAAGATGGAACTGCTGATGGAATGAGGCTTGTTCAGCTCCTCATTGCTTGCGCTGAGGCTGTCGCTTGCCGCGACAAGTCACACGCATCTGCTTTGCTATCTGAGCTTCGTGCTAATGCTTTGGTGTTTGGCTCTTCGTTCCAGCGTGTGGCGTCTTGCTTTGTCCAAGGTTTAGCCAGCAGGCTAGCCTTGGTTCAACCGCTAGGGGCAGTTGGTTTTGTTCCATCGAAACTGAATGCGAAAGATTATGCTCTGATGGACAAGAAAGAAGAGGCATTGCGCCTTGTTTATGAAATTTGCCCACATATTCAATTTGGTCATTTTGTGGCCAACTCATCAATATTGGAAGCCTTTGAGGGAGAGAGTTTCGTTCATGTGGTAGACCTTGGGATGACCCTAGGTTTGCCACATGGCGATCAGTGGCGAGGCCTGATCCATAGCCTCACCACACGTGCAGGCCAGCCACCAGTGTCGCGTCTTCGGATAACTGGTGTTGGCCTGTGTGCTGACAGATTGCAAATTATAGGAGAAGAGCTTGAGGCTTATGCTGACAGCTTGGGATTAAGTGTGGAGTTTACTTGTGTGGAAAGCAACTTGGAAAACTTAAAGCCTGAAGACATCAAACTTTATGAGGGTGAAGTACTAGTTGTCAATAGCATTCTTCAGTTGCATTGTGTAGTGAAAGAAAGTAGGGGAGCTCTCAATTCAGTTCTTCAGATGATCCATGAGCTCTCGCCGAAAGTCTTAGTTCTTGTTGAGCAGGACTCAAGCCACAATGGACCTTTTTTTCTAGGGAGATTTATGGAAGCATTGCATTACTACTCTGCAATATTTGACTCACTAGATGCAATGCTGCCCAAATATGACACTAGGCGTGCCAAGATGGAGCAATTCTACTTTGCTGAGGAGATCAAGAACATCATCAGCTGTGAGGGACCGGCACGCGTGGAGAGGCATGAGAGGGTTGATCAGTGGCGCAGGAGGATGAGCCGTGCCGGGTTCCAGGGTGCACCAATCAAGATGATGGCTCAGGCAAAGCAGTGGCTTGGGAAGAACAAGGTTTGTGAGGGCTACACTGTTGTGGAAGAAAAGGGTTGCTTGGTTCTTGGTTGGAAATCAAGGCCTATTGTTGCTGCTTCTTGCTGGAAATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

534

Amino Acids

59.34

Weight (kDa)

6.04

Isoelectric Point (pI)

55.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 159 - 532 2.4e-113 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014738)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g07610
malus_domestica MD06G1148900.v1.1 MD14G1162800.v1.1
prunus_persica Prupe.5G156100_v2.0.a1
pyrus_communis pycom06g13830
rosa_chinensis RchiOBHm_Chr7g0190881
rosa_laevigata RLG00000004489
rosa_multiflora Rmu_sc0004443.1_g000019 Rmu_sc0006903.1_g000003
rosa_roxburghii Rroxscaffold_3G00264230
rosa_rugosa Rorug06G0507700
rosa_samantha Rh1DG013400 Rh1DG013500 Rh7AG114400 Rh7CG119900 Rh7DG118200
rosa_wichuraiana Rw7G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 375
AccB1I GGYRCC 1 cut(s) 375
AccB7I CCANNNNNTGG 1 cut(s) 595
AccI GTMKAC 1 cut(s) 828
AccII CGCG 3 cut(s) 519, 922, 1392
AciI CCGC 5 cut(s) 285, 298, 410, 517, 647
AclWI GGATC 3 cut(s) 357, 874, 1162
AcoI YGGCCR 3 cut(s) 10, 16, 774
AcsI RAATTY 1 cut(s) 741
AcuI CTGAAG 3 cut(s) 1080, 1094, 1145
AcvI CACGTG 1 cut(s) 899
AcyI GRCGYC 1 cut(s) 599
AfaI GTAC 3 cut(s) 310, 377, 1089
AfiI CCNNNNNNNGG 3 cut(s) 14, 333, 595
AflIII ACRYGT 2 cut(s) 896, 1390
AgsI TTSAA 2 cut(s) 644, 758
AhdI GACNNNNNGTC 1 cut(s) 259
AhlI ACTAGT 1 cut(s) 1090
AjnI CCWGG 3 cut(s) 7, 153, 1449
AleI CACNNNNGTG 1 cut(s) 1526
Alw21I GWGCWC 5 cut(s) 39, 47, 1147, 1179, 1459
Alw44I GTGCAC 2 cut(s) 43, 1455
AlwI GGATC 3 cut(s) 357, 874, 1162
AoxI GGCC 7 cut(s) 10, 16, 774, 874, 904, 943, 1568
ApaLI GTGCAC 2 cut(s) 43, 1455
ApeKI GCWGC 7 cut(s) 300, 303, 383, 404, 407, 1300, 1580
ApoI RAATTY 1 cut(s) 741
ArsI GACNNNNNNTTYG 2 cut(s) 832, 864
Asp718I GGTACC 1 cut(s) 375
AspA2I CCTAGG 1 cut(s) 844
AspLEI GCGC 3 cut(s) 500, 729, 1425
AspS9I GGNCC 3 cut(s) 217, 1226, 1382
AsuC2I CCSGG 3 cut(s) 215, 347, 1444
AsuHPI GGTGA 2 cut(s) 883, 1094
AsuNHI GCTAGC 1 cut(s) 631
AvaII GGWCC 3 cut(s) 217, 1226, 1382
AvrII CCTAGG 1 cut(s) 844
BaeGI GKGCMC 2 cut(s) 47, 1459
BaeI ACNNNNGTAYC 2 cut(s) 359, 392
BalI TGGCCA 2 cut(s) 12, 776
BanI GGYRCC 1 cut(s) 375
BanII GRGCYC 2 cut(s) 1147, 1179
BauI CACGAG 1 cut(s) 38
BbrPI CACGTG 1 cut(s) 899
BbsI GAAGAC 2 cut(s) 917, 1068
Bbv12I GWGCWC 5 cut(s) 39, 47, 1147, 1179, 1459
BbvCI CCTCAGC 2 cut(s) 501, 1350
BbvI GCAGC 7 cut(s) 312, 315, 391, 395, 419, 1287, 1567
BccI CCATC 6 cut(s) 446, 458, 679, 697, 1324, 1465
BceAI ACGGC 2 cut(s) 298, 1422
BciT130I CCWGG 3 cut(s) 9, 155, 1451
BclI TGATCA 4 cut(s) 49, 186, 417, 1414
BcnI CCSGG 3 cut(s) 215, 347, 1444
BcuI ACTAGT 1 cut(s) 1090
BfaI CTAG 7 cut(s) 632, 650, 845, 1091, 1238, 1289, 1317
BfmI CTRYAG 1 cut(s) 405
BlnI CCTAGG 1 cut(s) 844
BmcAI AGTACT 2 cut(s) 310, 1089
Bme1390I CCNGG 6 cut(s) 9, 155, 215, 347, 1444, 1451
Bme18I GGWCC 3 cut(s) 217, 1226, 1382
BmeRI GACNNNNNGTC 1 cut(s) 259
BmgT120I GGNCC 3 cut(s) 217, 1226, 1382
BmiI GGNNCC 5 cut(s) 6, 218, 377, 1383, 1448
BmrFI CCNGG 6 cut(s) 9, 155, 215, 347, 1444, 1451
BmsI GCATC 3 cut(s) 152, 542, 1282
BmtI GCTAGC 1 cut(s) 635
BpiI GAAGAC 2 cut(s) 917, 1068
BplI GAGNNNNNCTC 2 cut(s) 1196, 1228
Bpu10I CCTNAGC 3 cut(s) 501, 1350, 1476
BpuEI CTTGAG 2 cut(s) 1004, 1198
BpuMI CCSGG 3 cut(s) 215, 347, 1444
Bsa29I ATCGAT 1 cut(s) 249
BsaAI YACGTR 1 cut(s) 899
BsaHI GRCGYC 1 cut(s) 599
BsaJI CCNNGG 9 cut(s) 13, 91, 332, 426, 613, 636, 832, 844, 1450
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 333, 595
Bse118I RCCGGY 1 cut(s) 1384
Bse1I ACTGG 3 cut(s) 273, 914, 940
Bse3DI GCAATG 4 cut(s) 489, 722, 1256, 1302
BseBI CCWGG 3 cut(s) 9, 155, 1451
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 9 cut(s) 13, 91, 332, 426, 613, 636, 832, 844, 1450
BseGI GGATG 2 cut(s) 843, 1437
BseLI CCNNNNNNNGG 3 cut(s) 14, 333, 595
BseMI GCAATG 4 cut(s) 489, 722, 1256, 1302
BseMII CTCAG 4 cut(s) 492, 540, 1341, 1490
BseNI ACTGG 3 cut(s) 273, 914, 940
BseRI GAGGAG 3 cut(s) 413, 476, 1367
BseSI GKGCMC 2 cut(s) 47, 1459
BseXI GCAGC 7 cut(s) 312, 315, 391, 395, 419, 1287, 1567
BsgI GTGCAG 1 cut(s) 921
Bsh1236I CGCG 3 cut(s) 519, 922, 1392
BshFI GGCC 7 cut(s) 12, 18, 776, 876, 906, 945, 1570
BshNI GGYRCC 1 cut(s) 375
BshVI ATCGAT 1 cut(s) 249
BsiHKAI GWGCWC 5 cut(s) 39, 47, 1147, 1179, 1459
BsiSI CCGG 4 cut(s) 214, 346, 1385, 1443
BslFI GGGAC 2 cut(s) 230, 1395
BslI CCNNNNNNNGG 3 cut(s) 14, 333, 595
BsmFI GGGAC 2 cut(s) 230, 1395
BsmI GAATGC 2 cut(s) 688, 1104
BsnI GGCC 7 cut(s) 12, 18, 776, 876, 906, 945, 1570
Bsp1286I GDGCHC 5 cut(s) 39, 47, 1147, 1179, 1459
Bsp143I GATC 9 cut(s) 49, 186, 349, 417, 862, 879, 1167, 1356, 1414
Bsp19I CCATGG 2 cut(s) 13, 91
BspACI CCGC 5 cut(s) 285, 298, 410, 517, 647
BspANI GGCC 7 cut(s) 12, 18, 776, 876, 906, 945, 1570
BspCNI CTCAG 4 cut(s) 493, 541, 1342, 1489
BspDI ATCGAT 1 cut(s) 249
BspFNI CGCG 3 cut(s) 519, 922, 1392
BspHI TCATGA 3 cut(s) 27, 52, 205
BspLI GGNNCC 5 cut(s) 6, 218, 377, 1383, 1448
BspMAI CTGCAG 1 cut(s) 409
BspOI GCTAGC 1 cut(s) 635
BspPI GGATC 3 cut(s) 357, 874, 1162
BspQI GCTCTTC 2 cut(s) 586, 972
BspT107I GGYRCC 1 cut(s) 375
BsrDI GCAATG 4 cut(s) 489, 722, 1256, 1302
BsrFI RCCGGY 1 cut(s) 1384
BsrI ACTGG 3 cut(s) 273, 914, 940
BssAI RCCGGY 1 cut(s) 1384
BssECI CCNNGG 9 cut(s) 13, 91, 332, 426, 613, 636, 832, 844, 1450
BssMI GATC 9 cut(s) 49, 186, 349, 417, 862, 879, 1167, 1356, 1414
BssNI GRCGYC 1 cut(s) 599
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 7 cut(s) 13, 91, 332, 613, 636, 832, 844
Bst2BI CACGAG 1 cut(s) 38
Bst2UI CCWGG 3 cut(s) 9, 155, 1451
Bst4CI ACNGT 4 cut(s) 221, 261, 375, 1525
Bst6I CTCTTC 3 cut(s) 586, 711, 972
BstACI GRCGYC 1 cut(s) 599
BstBAI YACGTR 1 cut(s) 899
BstDEI CTNAG 5 cut(s) 501, 549, 1192, 1350, 1476
BstDSI CCRYGG 2 cut(s) 13, 91
BstENI CCTNNNNNAGG 1 cut(s) 331
BstF5I GGATG 2 cut(s) 843, 1437
BstFNI CGCG 3 cut(s) 519, 922, 1392
BstHHI GCGC 3 cut(s) 500, 729, 1425
BstKTI GATC 9 cut(s) 52, 189, 352, 420, 865, 882, 1170, 1359, 1417
BstMBI GATC 9 cut(s) 49, 186, 349, 417, 862, 879, 1167, 1356, 1414
BstMWI GCNNNNNNNGC 7 cut(s) 294, 303, 389, 410, 481, 550, 1596
BstNI CCWGG 3 cut(s) 9, 155, 1451
BstSCI CCNGG 6 cut(s) 7, 153, 213, 345, 1442, 1449
BstSFI CTRYAG 1 cut(s) 405
BstSLI GKGCMC 2 cut(s) 47, 1459
BstUI CGCG 3 cut(s) 519, 922, 1392
BstV1I GCAGC 7 cut(s) 312, 315, 391, 395, 419, 1287, 1567
BstV2I GAAGAC 2 cut(s) 917, 1068
Bsu15I ATCGAT 1 cut(s) 249
BsuRI GGCC 7 cut(s) 12, 18, 776, 876, 906, 945, 1570
BsuTUI ATCGAT 1 cut(s) 249
BtgI CCRYGG 2 cut(s) 13, 91
BtsCI GGATG 2 cut(s) 843, 1437
BtsI GCAGTG 2 cut(s) 169, 1493
BtsIMutI CAGTG 8 cut(s) 45, 169, 257, 872, 921, 1424, 1493, 1521
CciI TCATGA 3 cut(s) 27, 52, 205
CfoI GCGC 3 cut(s) 500, 729, 1425
Cfr10I RCCGGY 1 cut(s) 1384
Cfr13I GGNCC 3 cut(s) 217, 1226, 1382
ClaI ATCGAT 1 cut(s) 249
CseI GACGC 2 cut(s) 588, 911
Csp6I GTAC 3 cut(s) 309, 376, 1088
CviQI GTAC 3 cut(s) 309, 376, 1088
DdeI CTNAG 5 cut(s) 501, 549, 1192, 1350, 1476
DpnI GATC 9 cut(s) 51, 188, 351, 419, 864, 881, 1169, 1358, 1416
DpnII GATC 9 cut(s) 49, 186, 349, 417, 862, 879, 1167, 1356, 1414
DriI GACNNNNNGTC 1 cut(s) 259
EaeI YGGCCR 3 cut(s) 10, 16, 774
Eam1104I CTCTTC 3 cut(s) 586, 711, 972
Eam1105I GACNNNNNGTC 1 cut(s) 259
EarI CTCTTC 3 cut(s) 586, 711, 972
Ecl136II GAGCTC 2 cut(s) 1145, 1177
Eco130I CCWWGG 7 cut(s) 13, 91, 332, 613, 636, 832, 844
Eco147I AGGCCT 2 cut(s) 876, 1570
Eco24I GRGCYC 2 cut(s) 1147, 1179
Eco47I GGWCC 3 cut(s) 217, 1226, 1382
Eco53kI GAGCTC 2 cut(s) 1145, 1177
Eco57I CTGAAG 3 cut(s) 1080, 1094, 1145
Eco72I CACGTG 1 cut(s) 899
EcoICRI GAGCTC 2 cut(s) 1145, 1177
EcoNI CCTNNNNNAGG 1 cut(s) 331
EcoRII CCWGG 3 cut(s) 7, 153, 1449
EcoT14I CCWWGG 7 cut(s) 13, 91, 332, 613, 636, 832, 844
EcoT38I GRGCYC 2 cut(s) 1147, 1179
ErhI CCWWGG 7 cut(s) 13, 91, 332, 613, 636, 832, 844
FalI AAGNNNNNCTT 2 cut(s) 1529, 1561
FaqI GGGAC 2 cut(s) 230, 1395
FbaI TGATCA 4 cut(s) 49, 186, 417, 1414
FblI GTMKAC 1 cut(s) 828
FokI GGATG 2 cut(s) 850, 1444
FriOI GRGCYC 2 cut(s) 1147, 1179
FspBI CTAG 7 cut(s) 632, 650, 845, 1091, 1238, 1289, 1317
GlaI GCGC 3 cut(s) 499, 728, 1424
HaeIII GGCC 7 cut(s) 12, 18, 776, 876, 906, 945, 1570
HapII CCGG 4 cut(s) 214, 346, 1385, 1443
HgaI GACGC 2 cut(s) 588, 911
HhaI GCGC 3 cut(s) 500, 729, 1425
Hin1I GRCGYC 1 cut(s) 599
Hin6I GCGC 3 cut(s) 498, 727, 1423
HinP1I GCGC 3 cut(s) 498, 727, 1423
HinfI GANTC 5 cut(s) 100, 173, 254, 1211, 1283
HpaII CCGG 4 cut(s) 214, 346, 1385, 1443
HphI GGTGA 2 cut(s) 883, 1094
Hpy166II GTNNAC 4 cut(s) 45, 829, 1023, 1457
Hpy188I TCNGA 5 cut(s) 99, 550, 702, 930, 1164
Hpy188III TCNNGA 5 cut(s) 28, 53, 206, 1360, 1465
Hpy8I GTNNAC 4 cut(s) 45, 829, 1023, 1457
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 4 cut(s) 221, 261, 375, 1525
HpyCH4IV ACGT 2 cut(s) 22, 898
HpyF10VI GCNNNNNNNGC 7 cut(s) 294, 303, 389, 410, 481, 550, 1596
HpyF3I CTNAG 5 cut(s) 501, 549, 1192, 1350, 1476
HpySE526I ACGT 2 cut(s) 22, 898
Hsp92I GRCGYC 1 cut(s) 599
HspAI GCGC 3 cut(s) 498, 727, 1423
KpnI GGTACC 1 cut(s) 379
Ksp22I TGATCA 4 cut(s) 49, 186, 417, 1414
Kzo9I GATC 9 cut(s) 49, 186, 349, 417, 862, 879, 1167, 1356, 1414
LguI GCTCTTC 2 cut(s) 586, 972
LmnI GCTCC 6 cut(s) 10, 34, 437, 489, 1142, 1333
Lsp1109I GCAGC 7 cut(s) 312, 315, 391, 395, 419, 1287, 1567
LweI GCATC 3 cut(s) 152, 542, 1282
MaeI CTAG 7 cut(s) 632, 650, 845, 1091, 1238, 1289, 1317
MaeII ACGT 2 cut(s) 22, 898
MaeIII GTNAC 2 cut(s) 77, 525
MalI GATC 9 cut(s) 51, 188, 351, 419, 864, 881, 1169, 1358, 1416
MboI GATC 9 cut(s) 49, 186, 349, 417, 862, 879, 1167, 1356, 1414
MhlI GDGCHC 5 cut(s) 39, 47, 1147, 1179, 1459
MlsI TGGCCA 2 cut(s) 12, 776
MluCI AATT 6 cut(s) 57, 741, 758, 966, 1150, 1337
MluI ACGCGT 1 cut(s) 1390
MluNI TGGCCA 2 cut(s) 12, 776
MlyI GAGTC 3 cut(s) 248, 1205, 1277
MmeI TCCRAC 1 cut(s) 1538
Mox20I TGGCCA 2 cut(s) 12, 776
MscI TGGCCA 2 cut(s) 12, 776
MseI TTAA 2 cut(s) 1010, 1052
MslI CAYNNNNRTG 1 cut(s) 1526
Msp20I TGGCCA 2 cut(s) 12, 776
MspA1I CMGCKG 2 cut(s) 410, 1374
MspI CCGG 4 cut(s) 214, 346, 1385, 1443
MspR9I CCNGG 6 cut(s) 9, 155, 215, 347, 1444, 1451
Mva1269I GAATGC 2 cut(s) 688, 1104
MvaI CCWGG 3 cut(s) 9, 155, 1451
MvnI CGCG 3 cut(s) 519, 922, 1392
MwoI GCNNNNNNNGC 7 cut(s) 294, 303, 389, 410, 481, 550, 1596
NciI CCSGG 3 cut(s) 215, 347, 1444
NcoI CCATGG 2 cut(s) 13, 91
NdeII GATC 9 cut(s) 49, 186, 349, 417, 862, 879, 1167, 1356, 1414
NheI GCTAGC 1 cut(s) 631
NlaIV GGNNCC 5 cut(s) 6, 218, 377, 1383, 1448
NmeAIII GCCGAG 1 cut(s) 451
NmuCI GTSAC 2 cut(s) 77, 525
OliI CACNNNNGTG 1 cut(s) 1526
PagI TCATGA 3 cut(s) 27, 52, 205
PceI AGGCCT 2 cut(s) 876, 1570
PciSI GCTCTTC 2 cut(s) 586, 972
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PctI GAATGC 2 cut(s) 688, 1104
PfeI GAWTC 2 cut(s) 100, 173
PflMI CCANNNNNTGG 1 cut(s) 595
PfoI TCCNGGA 1 cut(s) 345
PleI GAGTC 3 cut(s) 248, 1205, 1277
PmaCI CACGTG 1 cut(s) 899
PmlI CACGTG 1 cut(s) 899
PpsI GAGTC 3 cut(s) 248, 1205, 1277
Ppu21I YACGTR 1 cut(s) 899
Psp124BI GAGCTC 2 cut(s) 1147, 1179
Psp6I CCWGG 3 cut(s) 7, 153, 1449
PspCI CACGTG 1 cut(s) 899
PspGI CCWGG 3 cut(s) 7, 153, 1449
PspN4I GGNNCC 5 cut(s) 6, 218, 377, 1383, 1448
PspPI GGNCC 3 cut(s) 217, 1226, 1382
PstI CTGCAG 1 cut(s) 409
PvuII CAGCTG 1 cut(s) 1374
RsaI GTAC 3 cut(s) 310, 377, 1089
RsaNI GTAC 3 cut(s) 309, 376, 1088
RseI CAYNNNNRTG 1 cut(s) 1526
SacI GAGCTC 2 cut(s) 1147, 1179
SapI GCTCTTC 2 cut(s) 586, 972
SaqAI TTAA 2 cut(s) 1010, 1052
Sau3AI GATC 9 cut(s) 49, 186, 349, 417, 862, 879, 1167, 1356, 1414
Sau96I GGNCC 3 cut(s) 217, 1226, 1382
ScaI AGTACT 2 cut(s) 310, 1089
SchI GAGTC 3 cut(s) 248, 1205, 1277
ScrFI CCNGG 6 cut(s) 9, 155, 215, 347, 1444, 1451
SduI GDGCHC 5 cut(s) 39, 47, 1147, 1179, 1459
SfaNI GCATC 3 cut(s) 152, 542, 1282
SfcI CTRYAG 1 cut(s) 405
SinI GGWCC 3 cut(s) 217, 1226, 1382
SmiMI CAYNNNNRTG 1 cut(s) 1526
SmlI CTYRAG 2 cut(s) 983, 1213
SmoI CTYRAG 2 cut(s) 983, 1213
SpeI ACTAGT 1 cut(s) 1090
Sse9I AATT 6 cut(s) 57, 741, 758, 966, 1150, 1337
SseBI AGGCCT 2 cut(s) 876, 1570
SsiI CCGC 5 cut(s) 285, 298, 410, 517, 647
SspI AATATT 2 cut(s) 789, 1278
SspMI CTAG 7 cut(s) 632, 650, 845, 1091, 1238, 1289, 1317
SstI GAGCTC 2 cut(s) 1147, 1179
StuI AGGCCT 2 cut(s) 876, 1570
StyD4I CCNGG 6 cut(s) 7, 153, 213, 345, 1442, 1449
StyI CCWWGG 7 cut(s) 13, 91, 332, 613, 636, 832, 844
TaaI ACNGT 4 cut(s) 221, 261, 375, 1525
TaiI ACGT 2 cut(s) 25, 901
TaqI TCGA 5 cut(s) 70, 84, 249, 263, 674
TasI AATT 6 cut(s) 57, 741, 758, 966, 1150, 1337
TatI WGTACW 2 cut(s) 308, 1087
TauI GCSGC 4 cut(s) 288, 300, 413, 519
TfiI GAWTC 2 cut(s) 100, 173
Tru1I TTAA 2 cut(s) 1010, 1052
Tru9I TTAA 2 cut(s) 1010, 1052
TscAI CASTG 8 cut(s) 52, 169, 264, 872, 921, 1424, 1493, 1528
TseFI GTSAC 2 cut(s) 77, 525
TseI GCWGC 7 cut(s) 300, 303, 383, 404, 407, 1300, 1580
Tsp45I GTSAC 2 cut(s) 77, 525
TspDTI ATGAA 9 cut(s) 16, 69, 96, 117, 194, 222, 329, 753, 809
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 8 cut(s) 52, 169, 264, 872, 921, 1424, 1493, 1528
Van91I CCANNNNNTGG 1 cut(s) 595
VneI GTGCAC 2 cut(s) 43, 1455
VpaK11BI GGWCC 3 cut(s) 217, 1226, 1382
XagI CCTNNNNNAGG 1 cut(s) 331
XapI RAATTY 1 cut(s) 741
XmaJI CCTAGG 1 cut(s) 844
XmiI GTMKAC 1 cut(s) 828
XspI CTAG 7 cut(s) 632, 650, 845, 1091, 1238, 1289, 1317
ZrmI AGTACT 2 cut(s) 310, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.