Rh7DG118200

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0014738
Physical Location & Seq
Forward (+)
9957708 .. 9959110
1403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG118200.1

Sequence Viewer

Length: 1188 bp
ATGATCAATGCCGAGGAAAATGGAGCTGAACAAGAAGATGGAACTGCTGATGGCATGAGGCTTGTTCAGCTGCTCATTGCTTGTGCTGAAGCTGTTGCTTGCCGCGACAAGTCACATGCATCTGCTTTACTATCCGAGCTGCGTGCCAATGCTTTGGTGTTTGGCTCTTCGTTCCAGCGCGTGGCGTCTTGCTTTGTCCAAGGCCTAGCCAGCCGGCTAGCCTTGGTTCAACCGCTCGGGGCAGTTGGTTTTGTTCCATCAAAATTGAATGCGAAAGATTATGCTCTAATGGACAAGAAGGAAGAGGCATTGCGCCTTGTTTATGAAATTTGCCCACATATTCAATTTGGTCATTTTGTGGCCAACTCATCAATATTGGAAGCCCTTGAGGGAGAGAGTTTTGTTCATGTGGTAGACCTTGGGATGACCCTTGGTCTGCCACATGGCGATCAGTGGCAAGGCCTGATCCAAAGCCTTGCTACACGCGCAGGCCAGCCACCAGTGTCGCGCCTTCGGATAACTGGTGTTGGTCTGTGCGCTGACAGATTGCTAATTATAGGAGAAGAGCTCGAGGCTTATGCTGACAGCTTAGGAATAAATCTGGAGTTTTCTTGTGTAGAAAGCAACTTGGAAAACCTGAAGCCTGAAGACATCAAAATTTTTGAGGGTGAAGTATTAGTTGTCAATAGCATTCTTCAGTTGCACTGTGTGGTGAAAGAAAGCAGGGGAGCTCTCAATTCAGTTCTGCAGATGATTCATGAGCTCTCACCAAAAATCCTAGTTCTTGTTGAGCAGGACTCTAGCCACAATGGACCCTTTTTTCTTGGGAGGTTTATGGAAGCATTGCATTACTACTCTGCAATATTTGACTCACTAGATGCAATGCTTCCCAAATACGACACAAGGCGTGCCAAGATGGAGCAATTCTACTTTGCTGAGGAGATCAAGAACATCATCAGCTGTGAGGGACCAGCACGAGTGGAGAGGCATGAGAGGGTTGATCAGTGGCGCAGGAGGATGAGCCGTGCCGGGTTTCAGGGTGCACCGATTAAGATGATGGCTCAAGCAAAGCAGTGGCTAGGGAAGAACAAGGTTTGTGAGGGCTACACTGTTGTGGAAGAAAAGGGTTGCTTGGTTCTTGGTTGGAAATCAAGGCCTATTGTTGCTGCTTCTTGCTGGAAATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

395

Amino Acids

43.76

Weight (kDa)

5.77

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 21 - 394 3.8e-114 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014738)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g07610
malus_domestica MD06G1148900.v1.1 MD14G1162800.v1.1
prunus_persica Prupe.5G156100_v2.0.a1
pyrus_communis pycom06g13830
rosa_chinensis RchiOBHm_Chr7g0190881
rosa_laevigata RLG00000004489
rosa_multiflora Rmu_sc0004443.1_g000019 Rmu_sc0006903.1_g000003
rosa_roxburghii Rroxscaffold_3G00264230
rosa_rugosa Rorug06G0507700
rosa_samantha Rh1DG013400 Rh1DG013500 Rh7AG114400 Rh7CG119900 Rh7DG118200
rosa_wichuraiana Rw7G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 181
AccBSI CCGCTC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 414
AccII CGCG 4 cut(s) 105, 180, 486, 508
AciI CCGC 2 cut(s) 103, 233
AclWI GGATC 1 cut(s) 460
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 2 cut(s) 327, 657
AcuI CTGAAG 4 cut(s) 108, 659, 666, 680
AcyI GRCGYC 1 cut(s) 185
AdeI CACNNNGTG 1 cut(s) 709
AfiI CCNNNNNNNGG 1 cut(s) 181
AgsI TTSAA 3 cut(s) 230, 268, 344
AhdI GACNNNNNGTC 1 cut(s) 432
AleI CACNNNNGTG 1 cut(s) 1112
AluBI AGCT 9 cut(s) 26, 70, 92, 139, 568, 588, 731, 763, 960
AluI AGCT 9 cut(s) 26, 70, 92, 139, 568, 588, 731, 763, 960
Alw21I GWGCWC 4 cut(s) 570, 733, 765, 1045
Alw44I GTGCAC 1 cut(s) 1041
AlwI GGATC 1 cut(s) 460
Ama87I CYCGRG 2 cut(s) 236, 569
AoxI GGCC 5 cut(s) 202, 360, 460, 490, 1154
ApaLI GTGCAC 1 cut(s) 1041
ApeKI GCWGC 3 cut(s) 70, 139, 1166
ApoI RAATTY 2 cut(s) 327, 657
AspLEI GCGC 6 cut(s) 180, 315, 488, 510, 539, 1011
AspS9I GGNCC 2 cut(s) 812, 968
AsuC2I CCSGG 1 cut(s) 1030
AsuHPI GGTGA 3 cut(s) 680, 724, 759
AsuNHI GCTAGC 1 cut(s) 217
AvaI CYCGRG 2 cut(s) 236, 569
AvaII GGWCC 2 cut(s) 812, 968
BaeGI GKGCMC 1 cut(s) 1045
BalI TGGCCA 1 cut(s) 362
BanII GRGCYC 3 cut(s) 570, 733, 765
BauI CACGAG 1 cut(s) 975
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 4 cut(s) 570, 733, 765, 1045
BbvCI CCTCAGC 1 cut(s) 936
BbvI GCAGC 3 cut(s) 57, 126, 1153
BccI CCATC 5 cut(s) 32, 44, 265, 910, 1051
BceAI ACGGC 1 cut(s) 1008
BcgI CGANNNNNNTGC 4 cut(s) 125, 159, 560, 594
BclI TGATCA 2 cut(s) 3, 1000
BcnI CCSGG 1 cut(s) 1030
BfaI CTAG 6 cut(s) 206, 218, 779, 801, 875, 1079
BfmI CTRYAG 1 cut(s) 746
BisI GCNGC 4 cut(s) 71, 103, 140, 1167
BlsI GCNGC 4 cut(s) 72, 104, 141, 1168
Bme1390I CCNGG 1 cut(s) 1030
Bme18I GGWCC 2 cut(s) 812, 968
BmeRI GACNNNNNGTC 1 cut(s) 432
BmeT110I CYCGRG 2 cut(s) 236, 569
BmgT120I GGNCC 2 cut(s) 812, 968
BmiI GGNNCC 2 cut(s) 814, 969
BmrFI CCNGG 1 cut(s) 1030
BmsI GCATC 2 cut(s) 128, 868
BmtI GCTAGC 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 654
BplI GAGNNNNNCTC 4 cut(s) 552, 584, 782, 814
BpmI CTGGAG 1 cut(s) 623
Bpu10I CCTNAGC 2 cut(s) 589, 936
BpuEI CTTGAG 2 cut(s) 407, 1047
BpuMI CCSGG 1 cut(s) 1030
BsaHI GRCGYC 1 cut(s) 185
BsaJI CCNNGG 5 cut(s) 12, 199, 222, 418, 430
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse118I RCCGGY 1 cut(s) 213
Bse1I ACTGG 2 cut(s) 500, 526
Bse3DI GCAATG 4 cut(s) 75, 308, 842, 888
BseDI CCNNGG 5 cut(s) 12, 199, 222, 418, 430
BseGI GGATG 2 cut(s) 429, 1023
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMI GCAATG 4 cut(s) 75, 308, 842, 888
BseMII CTCAG 1 cut(s) 927
BseNI ACTGG 2 cut(s) 500, 526
BseRI GAGGAG 1 cut(s) 953
BseSI GKGCMC 1 cut(s) 1045
BseXI GCAGC 3 cut(s) 57, 126, 1153
Bsh1236I CGCG 4 cut(s) 105, 180, 486, 508
BshFI GGCC 5 cut(s) 204, 362, 462, 492, 1156
BsiHKAI GWGCWC 4 cut(s) 570, 733, 765, 1045
BsiHKCI CYCGRG 2 cut(s) 236, 569
BsiSI CCGG 2 cut(s) 214, 1029
BslFI GGGAC 1 cut(s) 981
BslI CCNNNNNNNGG 1 cut(s) 181
BsmFI GGGAC 1 cut(s) 981
BsmI GAATGC 2 cut(s) 274, 690
BsnI GGCC 5 cut(s) 204, 362, 462, 492, 1156
BsoBI CYCGRG 2 cut(s) 236, 569
Bsp1286I GDGCHC 4 cut(s) 570, 733, 765, 1045
Bsp143I GATC 5 cut(s) 3, 448, 465, 942, 1000
BspACI CCGC 2 cut(s) 103, 233
BspANI GGCC 5 cut(s) 204, 362, 462, 492, 1156
BspCNI CTCAG 1 cut(s) 928
BspFNI CGCG 4 cut(s) 105, 180, 486, 508
BspHI TCATGA 1 cut(s) 757
BspLI GGNNCC 2 cut(s) 814, 969
BspMAI CTGCAG 1 cut(s) 750
BspOI GCTAGC 1 cut(s) 221
BspPI GGATC 1 cut(s) 460
BspQI GCTCTTC 2 cut(s) 172, 558
BsrBI CCGCTC 1 cut(s) 235
BsrDI GCAATG 4 cut(s) 75, 308, 842, 888
BsrFI RCCGGY 1 cut(s) 213
BsrI ACTGG 2 cut(s) 500, 526
BssAI RCCGGY 1 cut(s) 213
BssECI CCNNGG 5 cut(s) 12, 199, 222, 418, 430
BssMI GATC 5 cut(s) 3, 448, 465, 942, 1000
BssNI GRCGYC 1 cut(s) 185
BssSI CACGAG 1 cut(s) 975
BssT1I CCWWGG 4 cut(s) 199, 222, 418, 430
Bst2BI CACGAG 1 cut(s) 975
Bst4CI ACNGT 2 cut(s) 707, 1111
Bst6I CTCTTC 3 cut(s) 172, 297, 558
BstACI GRCGYC 1 cut(s) 185
BstC8I GCNNGC 8 cut(s) 100, 144, 211, 215, 219, 490, 494, 909
BstDEI CTNAG 2 cut(s) 589, 936
BstF5I GGATG 2 cut(s) 429, 1023
BstFNI CGCG 4 cut(s) 105, 180, 486, 508
BstHHI GCGC 6 cut(s) 180, 315, 488, 510, 539, 1011
BstKTI GATC 5 cut(s) 6, 451, 468, 945, 1003
BstMBI GATC 5 cut(s) 3, 448, 465, 942, 1000
BstMWI GCNNNNNNNGC 4 cut(s) 67, 210, 485, 1182
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 1028
BstSFI CTRYAG 1 cut(s) 746
BstSLI GKGCMC 1 cut(s) 1045
BstUI CGCG 4 cut(s) 105, 180, 486, 508
BstV1I GCAGC 3 cut(s) 57, 126, 1153
BstV2I GAAGAC 1 cut(s) 654
BstXI CCANNNNNNTGG 1 cut(s) 154
BsuRI GGCC 5 cut(s) 204, 362, 462, 492, 1156
BtsCI GGATG 2 cut(s) 429, 1023
BtsI GCAGTG 1 cut(s) 1079
BtsIMutI CAGTG 6 cut(s) 458, 507, 703, 1010, 1079, 1107
Cac8I GCNNGC 8 cut(s) 100, 144, 211, 215, 219, 490, 494, 909
CciI TCATGA 1 cut(s) 757
CfoI GCGC 6 cut(s) 180, 315, 488, 510, 539, 1011
Cfr10I RCCGGY 1 cut(s) 213
Cfr13I GGNCC 2 cut(s) 812, 968
CseI GACGC 1 cut(s) 174
CviAII CATG 6 cut(s) 55, 116, 407, 443, 758, 989
DdeI CTNAG 2 cut(s) 589, 936
DpnI GATC 5 cut(s) 5, 450, 467, 944, 1002
DpnII GATC 5 cut(s) 3, 448, 465, 942, 1000
DraIII CACNNNGTG 1 cut(s) 709
DriI GACNNNNNGTC 1 cut(s) 432
EaeI YGGCCR 1 cut(s) 360
Eam1104I CTCTTC 3 cut(s) 172, 297, 558
Eam1105I GACNNNNNGTC 1 cut(s) 432
EarI CTCTTC 3 cut(s) 172, 297, 558
Ecl136II GAGCTC 3 cut(s) 568, 731, 763
Eco130I CCWWGG 4 cut(s) 199, 222, 418, 430
Eco147I AGGCCT 3 cut(s) 204, 462, 1156
Eco24I GRGCYC 3 cut(s) 570, 733, 765
Eco47I GGWCC 2 cut(s) 812, 968
Eco53kI GAGCTC 3 cut(s) 568, 731, 763
Eco57I CTGAAG 4 cut(s) 108, 659, 666, 680
Eco88I CYCGRG 2 cut(s) 236, 569
EcoICRI GAGCTC 3 cut(s) 568, 731, 763
EcoT14I CCWWGG 4 cut(s) 199, 222, 418, 430
EcoT22I ATGCAT 1 cut(s) 121
EcoT38I GRGCYC 3 cut(s) 570, 733, 765
ErhI CCWWGG 4 cut(s) 199, 222, 418, 430
FaeI CATG 6 cut(s) 58, 119, 410, 446, 761, 992
FalI AAGNNNNNCTT 2 cut(s) 1115, 1147
FaqI GGGAC 1 cut(s) 981
FatI CATG 6 cut(s) 54, 115, 406, 442, 757, 988
FbaI TGATCA 2 cut(s) 3, 1000
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 4 cut(s) 71, 103, 140, 1167
FokI GGATG 2 cut(s) 436, 1030
FriOI GRGCYC 3 cut(s) 570, 733, 765
Fsp4HI GCNGC 4 cut(s) 71, 103, 140, 1167
FspBI CTAG 6 cut(s) 206, 218, 779, 801, 875, 1079
GlaI GCGC 6 cut(s) 179, 314, 487, 509, 538, 1010
GluI GCNGC 4 cut(s) 71, 103, 140, 1167
GsuI CTGGAG 1 cut(s) 623
HaeIII GGCC 5 cut(s) 204, 362, 462, 492, 1156
HapII CCGG 2 cut(s) 214, 1029
HgaI GACGC 1 cut(s) 174
HhaI GCGC 6 cut(s) 180, 315, 488, 510, 539, 1011
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 6 cut(s) 58, 119, 410, 446, 761, 992
Hin6I GCGC 6 cut(s) 178, 313, 486, 508, 537, 1009
HinP1I GCGC 6 cut(s) 178, 313, 486, 508, 537, 1009
HinfI GANTC 3 cut(s) 754, 797, 869
HpaII CCGG 2 cut(s) 214, 1029
HphI GGTGA 3 cut(s) 680, 724, 759
Hpy166II GTNNAC 2 cut(s) 415, 1043
Hpy188I TCNGA 2 cut(s) 136, 516
Hpy188III TCNNGA 3 cut(s) 602, 758, 946
Hpy8I GTNNAC 2 cut(s) 415, 1043
HpyAV CCTTC 2 cut(s) 292, 521
HpyCH4III ACNGT 2 cut(s) 707, 1111
HpyCH4V TGCA 7 cut(s) 119, 703, 748, 847, 860, 881, 1043
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 210, 485, 1182
HpyF3I CTNAG 2 cut(s) 589, 936
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 6 cut(s) 58, 119, 410, 446, 761, 992
HspAI GCGC 6 cut(s) 178, 313, 486, 508, 537, 1009
KroI GCCGGC 1 cut(s) 213
KroNI GCCGGC 1 cut(s) 215
Ksp22I TGATCA 2 cut(s) 3, 1000
Kzo9I GATC 5 cut(s) 3, 448, 465, 942, 1000
LguI GCTCTTC 2 cut(s) 172, 558
LmnI GCTCC 3 cut(s) 23, 728, 919
Lsp1109I GCAGC 3 cut(s) 57, 126, 1153
LweI GCATC 2 cut(s) 128, 868
MaeI CTAG 6 cut(s) 206, 218, 779, 801, 875, 1079
MaeIII GTNAC 1 cut(s) 111
MalI GATC 5 cut(s) 5, 450, 467, 944, 1002
MbiI CCGCTC 1 cut(s) 235
MboI GATC 5 cut(s) 3, 448, 465, 942, 1000
MboII GAAGA 8 cut(s) 47, 159, 314, 575, 659, 686, 1096, 1130
MhlI GDGCHC 4 cut(s) 570, 733, 765, 1045
MlsI TGGCCA 1 cut(s) 362
MluCI AATT 7 cut(s) 263, 327, 344, 552, 657, 736, 923
MluNI TGGCCA 1 cut(s) 362
MlyI GAGTC 2 cut(s) 791, 863
MmeI TCCRAC 1 cut(s) 1124
Mox20I TGGCCA 1 cut(s) 362
Mph1103I ATGCAT 1 cut(s) 121
MroNI GCCGGC 1 cut(s) 213
MscI TGGCCA 1 cut(s) 362
MseI TTAA 1 cut(s) 1050
MslI CAYNNNNRTG 1 cut(s) 1112
Msp20I TGGCCA 1 cut(s) 362
MspA1I CMGCKG 2 cut(s) 70, 960
MspI CCGG 2 cut(s) 214, 1029
MspR9I CCNGG 1 cut(s) 1030
Mva1269I GAATGC 2 cut(s) 274, 690
MvnI CGCG 4 cut(s) 105, 180, 486, 508
MwoI GCNNNNNNNGC 4 cut(s) 67, 210, 485, 1182
NaeI GCCGGC 1 cut(s) 215
NciI CCSGG 1 cut(s) 1030
NdeII GATC 5 cut(s) 3, 448, 465, 942, 1000
NgoMIV GCCGGC 1 cut(s) 213
NheI GCTAGC 1 cut(s) 217
NlaIII CATG 6 cut(s) 58, 119, 410, 446, 761, 992
NlaIV GGNNCC 2 cut(s) 814, 969
NmeAIII GCCGAG 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 111
NsiI ATGCAT 1 cut(s) 121
NspI RCATGY 1 cut(s) 119
OliI CACNNNNGTG 1 cut(s) 1112
PaeR7I CTCGAG 1 cut(s) 569
PagI TCATGA 1 cut(s) 757
PceI AGGCCT 3 cut(s) 204, 462, 1156
PciSI GCTCTTC 2 cut(s) 172, 558
PctI GAATGC 2 cut(s) 274, 690
PdiI GCCGGC 1 cut(s) 215
PfeI GAWTC 1 cut(s) 754
PflMI CCANNNNNTGG 1 cut(s) 181
PkrI GCNGC 4 cut(s) 72, 104, 141, 1168
PleI GAGTC 2 cut(s) 791, 863
PpsI GAGTC 2 cut(s) 791, 863
Psp124BI GAGCTC 3 cut(s) 570, 733, 765
PspN4I GGNNCC 2 cut(s) 814, 969
PspPI GGNCC 2 cut(s) 812, 968
PspXI VCTCGAGB 1 cut(s) 569
PstI CTGCAG 1 cut(s) 750
PvuII CAGCTG 2 cut(s) 70, 960
RseI CAYNNNNRTG 1 cut(s) 1112
SacI GAGCTC 3 cut(s) 570, 733, 765
SapI GCTCTTC 2 cut(s) 172, 558
SaqAI TTAA 1 cut(s) 1050
SatI GCNGC 4 cut(s) 71, 103, 140, 1167
Sau3AI GATC 5 cut(s) 3, 448, 465, 942, 1000
Sau96I GGNCC 2 cut(s) 812, 968
SchI GAGTC 2 cut(s) 791, 863
ScrFI CCNGG 1 cut(s) 1030
SduI GDGCHC 4 cut(s) 570, 733, 765, 1045
SfaNI GCATC 2 cut(s) 128, 868
SfcI CTRYAG 1 cut(s) 746
Sfr274I CTCGAG 1 cut(s) 569
SinI GGWCC 2 cut(s) 812, 968
SlaI CTCGAG 1 cut(s) 569
SmiMI CAYNNNNRTG 1 cut(s) 1112
SmlI CTYRAG 3 cut(s) 386, 569, 1062
SmoI CTYRAG 3 cut(s) 386, 569, 1062
Sse9I AATT 7 cut(s) 263, 327, 344, 552, 657, 736, 923
SseBI AGGCCT 3 cut(s) 204, 462, 1156
SsiI CCGC 2 cut(s) 103, 233
SspI AATATT 2 cut(s) 375, 864
SspMI CTAG 6 cut(s) 206, 218, 779, 801, 875, 1079
SstI GAGCTC 3 cut(s) 570, 733, 765
StuI AGGCCT 3 cut(s) 204, 462, 1156
StyD4I CCNGG 1 cut(s) 1028
StyI CCWWGG 4 cut(s) 199, 222, 418, 430
TaaI ACNGT 2 cut(s) 707, 1111
TaqI TCGA 1 cut(s) 570
TasI AATT 7 cut(s) 263, 327, 344, 552, 657, 736, 923
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 1 cut(s) 754
Tru1I TTAA 1 cut(s) 1050
Tru9I TTAA 1 cut(s) 1050
TscAI CASTG 6 cut(s) 458, 507, 710, 1010, 1079, 1114
TseFI GTSAC 1 cut(s) 111
TseI GCWGC 3 cut(s) 70, 139, 1166
Tsp45I GTSAC 1 cut(s) 111
TspDTI ATGAA 3 cut(s) 339, 395, 746
TspRI CASTG 6 cut(s) 458, 507, 710, 1010, 1079, 1114
Van91I CCANNNNNTGG 1 cut(s) 181
VneI GTGCAC 1 cut(s) 1041
VpaK11BI GGWCC 2 cut(s) 812, 968
XapI RAATTY 2 cut(s) 327, 657
XceI RCATGY 1 cut(s) 119
XhoI CTCGAG 1 cut(s) 569
XmiI GTMKAC 1 cut(s) 414
XspI CTAG 6 cut(s) 206, 218, 779, 801, 875, 1079
Zsp2I ATGCAT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.