MD14G1162800.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014738
Physical Location & Seq
Forward (+)
25718896 .. 25720694
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1162800.v1.1.491

Sequence Viewer

Length: 1644 bp
ATGATGTTTTGTGCAGGAGGGAGAAAAGAAGGTTACGTTCGAGAAATGGCACTGAGCCGAAACTTCCTGCCTGAGGAGTACATGATCAATCATGAAAGGACTAATAAAATGAGTGTCCTTGATCTCATTACTCACTCAGCTGCCTCAGCCATGCCTTGCTATCCTTATCCACACACGCCCTTGCTGGAGGAGGGCGGTACAGCTTCTACCCCCCCTTACTCGGATGAAAGCGGAAACCATAAGAGGCTGAAAAGGACTACAAGTATCTCCGACTCTATGTCAAGTCATAACAGTCTTTATAGTGGTGGAGGAAGCGGAGATAGTTGCATTACTGACATCAGCCTCAGTCGCCGAAGCAGCACGAATAGCTTGAATACCTTGCCAAGGCTTCATTTCCGGGACCATATTTGGACCTACACACAAAGGTACCTTGCAGCTGAAGCTGTTGAGGAGGCAGCGGCATCGATGTCCAATGCCGAGGGAAATGAAGAGGAGGAGGACGAAACCACTTATGGGATGAGGCTTGTTCAGCTCCTAATTGCTTGTGCTGAAGCTGTTGCTTGTCGCGACAAGTCACATGCCTCCGCGTTACTATCTGAGCTTCGGGCCAATGCCTTGGTCTTTGGCTCTTCATTCCAGCGTGTGGCATCATGTTTTGTCCAAGGCCTTGCCAACCGCCTAGCTCTGGTTCAACCGCTCGGGGCAGTAGGGTTCATTGGGTCCGCGATGAGCACAAAAGACTTTGCCTTGGACAAGAAGGAAGAGGCATTGCGCCTTGTTTATGAGATATGCCCCCACATTCAGTTTGGTCACTTTGTGGCCAATTCATCAATATTGGAAGCCTTTGAGGGAGAGAGTTTTGTTCATGTAGTAGACCTTGGGATGACCCTTGGTCTACCACATGGAGACCAGTGGCGAGGCTTGATCGAGAGCCTTGCCACACGCACAGGCCAGCCGCCTACACGCCTTAGAATAACCGGTGTTGGCCTTTACGGTGACAGATTGCAAATCATTGGAGAAGAGCTCGAGGCCTATGCCGACAGCTTGGGAATAAACCTGGAGTTTTCTGTGGTGGTAAGCAACTTGGAAAACCTGCGTCCGAAGGATATTAAAATGTATGATGGTGAAGTCCTTGTTGTCAATAGCATTCTTCAGTTGCATTGTGTGGTGAAAGAAAGCAGAGGAGCTCTCAATTCTGTCCTGCAGATGGTCCATGAGCTTTCGCCGAAAATCTTGGTTCTTGTGGAGCAAGACTCAAGCCACAATGGACCTTTTTTTTTGGGGAGGTTTATGGAGGCACTGCATTATTACTCTGCAATCTTTGACTCCCTAGATGCCATGCTGCCAAAATATGACACGAGGCGCGCGAAGATGGAGCAGTTCTACTTTGGTGAGGAGATTAAGAACATAATCAGCTGCGAGGGGCCGGCAAGGGTGGAGAGGCACGAGAGGGTTGATCAGTGGCGCCGGAGGATGAGCCGGGCAGGGTTTCAGGCTGCGCCGATTAAGATGCTGGCGCAGGCAAAGCAGTGGCTTGGGAAGATCAAGGTCTGTGAGGGTTACACTATTTTGGAAGAGAAGGGTTGCCTGGTTCTTGGTTGGAAATCTAAGCCCATTGTTGCTGCCTCCTGCTGGAAATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

548

Amino Acids

60.84

Weight (kDa)

6.05

Isoelectric Point (pI)

55.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 175 - 546 9.9e-114 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014738)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g07610
malus_domestica MD06G1148900.v1.1 MD14G1162800.v1.1
prunus_persica Prupe.5G156100_v2.0.a1
pyrus_communis pycom06g13830
rosa_chinensis RchiOBHm_Chr7g0190881
rosa_laevigata RLG00000004489
rosa_multiflora Rmu_sc0004443.1_g000019 Rmu_sc0006903.1_g000003
rosa_roxburghii Rroxscaffold_3G00264230
rosa_rugosa Rorug06G0507700
rosa_samantha Rh1DG013400 Rh1DG013500 Rh7AG114400 Rh7CG119900 Rh7DG118200
rosa_wichuraiana Rw7G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1101
Acc65I GGTACC 1 cut(s) 426
AccB1I GGYRCC 2 cut(s) 426, 1464
AccB7I CCANNNNNTGG 1 cut(s) 643
AccBSI CCGCTC 1 cut(s) 697
AccI GTMKAC 2 cut(s) 873, 895
AccII CGCG 5 cut(s) 567, 587, 725, 1365, 1367
AciI CCGC 9 cut(s) 195, 231, 315, 458, 585, 676, 695, 723, 956
AcoI YGGCCR 1 cut(s) 819
AcuI CTGAAG 3 cut(s) 459, 570, 1136
AcyI GRCGYC 1 cut(s) 1465
AdeI CACNNNGTG 1 cut(s) 817
AfaI GTAC 3 cut(s) 80, 199, 428
AfiI CCNNNNNNNGG 8 cut(s) 73, 220, 384, 513, 643, 685, 1207, 1632
AgeI ACCGGT 1 cut(s) 977
AgsI TTSAA 2 cut(s) 373, 692
AhdI GACNNNNNGTC 2 cut(s) 277, 891
AjnI CCWGG 2 cut(s) 1056, 1587
Alw21I GWGCWC 3 cut(s) 734, 1026, 1189
Alw26I GTCTC 1 cut(s) 900
Ama87I CYCGRG 2 cut(s) 698, 1025
AoxI GGCC 7 cut(s) 606, 664, 819, 949, 985, 1029, 1424
ApeKI GCWGC 8 cut(s) 140, 357, 434, 455, 1342, 1416, 1496, 1622
AsiGI ACCGGT 1 cut(s) 977
Asp718I GGTACC 1 cut(s) 426
AspLEI GCGC 6 cut(s) 774, 1365, 1367, 1467, 1501, 1519
AspS9I GGNCC 7 cut(s) 400, 411, 606, 720, 1210, 1268, 1424
AsuC2I CCSGG 2 cut(s) 398, 1481
AsuHPI GGTGA 4 cut(s) 1007, 1136, 1180, 1403
AvaI CYCGRG 2 cut(s) 698, 1025
AvaII GGWCC 5 cut(s) 400, 411, 720, 1210, 1268
AxyI CCTNAGG 1 cut(s) 72
BaeI ACNNNNGTAYC 2 cut(s) 410, 443
BalI TGGCCA 1 cut(s) 821
BanI GGYRCC 2 cut(s) 426, 1464
BanII GRGCYC 2 cut(s) 1026, 1189
BauI CACGAG 2 cut(s) 1357, 1445
Bbv12I GWGCWC 3 cut(s) 734, 1026, 1189
BbvCI CCTCAGC 1 cut(s) 145
BbvI GCAGC 8 cut(s) 127, 369, 446, 467, 1329, 1403, 1483, 1609
BccI CCATC 3 cut(s) 1115, 1201, 1366
BcgI CGANNNNNNTGC 8 cut(s) 444, 478, 917, 951, 1016, 1050, 1492, 1526
BciT130I CCWGG 2 cut(s) 1058, 1589
BclI TGATCA 2 cut(s) 84, 1456
BcnI CCSGG 2 cut(s) 398, 1481
BcoDI GTCTC 1 cut(s) 900
BfaI CTAG 2 cut(s) 680, 1331
BfmI CTRYAG 1 cut(s) 1202
BfoI RGCGCY 1 cut(s) 1468
BfuAI ACCTGC 1 cut(s) 1101
Bme1390I CCNGG 4 cut(s) 398, 1058, 1481, 1589
Bme18I GGWCC 5 cut(s) 400, 411, 720, 1210, 1268
BmeRI GACNNNNNGTC 2 cut(s) 277, 891
BmeT110I CYCGRG 2 cut(s) 698, 1025
BmgT120I GGNCC 7 cut(s) 400, 411, 606, 720, 1210, 1268, 1424
BmiI GGNNCC 5 cut(s) 401, 428, 721, 1425, 1466
BmrFI CCNGG 4 cut(s) 398, 1058, 1481, 1589
BmsI GCATC 4 cut(s) 470, 656, 1324, 1500
BplI GAGNNNNNCTC 6 cut(s) 1008, 1040, 1173, 1205, 1238, 1270
BpmI CTGGAG 2 cut(s) 206, 1079
Bpu10I CCTNAGC 1 cut(s) 145
BpuEI CTTGAG 1 cut(s) 1240
BpuMI CCSGG 2 cut(s) 398, 1481
Bsa29I ATCGAT 1 cut(s) 464
BsaHI GRCGYC 1 cut(s) 1465
BsaI GGTCTC 1 cut(s) 900
BsaJI CCNNGG 7 cut(s) 383, 477, 615, 661, 747, 877, 889
BsaWI WCCGGW 1 cut(s) 977
Bsc4I CCNNNNNNNGG 8 cut(s) 73, 220, 384, 513, 643, 685, 1207, 1632
Bse118I RCCGGY 2 cut(s) 977, 1426
Bse1I ACTGG 1 cut(s) 910
Bse21I CCTNAGG 1 cut(s) 72
Bse3DI GCAATG 1 cut(s) 767
BseBI CCWGG 2 cut(s) 1058, 1589
BseCI ATCGAT 1 cut(s) 464
BseDI CCNNGG 7 cut(s) 383, 477, 615, 661, 747, 877, 889
BseGI GGATG 4 cut(s) 229, 522, 888, 1479
BseLI CCNNNNNNNGG 8 cut(s) 73, 220, 384, 513, 643, 685, 1207, 1632
BseMI GCAATG 1 cut(s) 767
BseMII CTCAG 6 cut(s) 44, 63, 150, 159, 358, 588
BseNI ACTGG 1 cut(s) 910
BsePI GCGCGC 1 cut(s) 1363
BseRI GAGGAG 7 cut(s) 89, 203, 464, 506, 509, 1197, 1409
BseXI GCAGC 8 cut(s) 127, 369, 446, 467, 1329, 1403, 1483, 1609
BsgI GTGCAG 1 cut(s) 33
Bsh1236I CGCG 5 cut(s) 567, 587, 725, 1365, 1367
BshFI GGCC 7 cut(s) 608, 666, 821, 951, 987, 1031, 1426
BshNI GGYRCC 2 cut(s) 426, 1464
BshTI ACCGGT 1 cut(s) 977
BshVI ATCGAT 1 cut(s) 464
BsiHKAI GWGCWC 3 cut(s) 734, 1026, 1189
BsiHKCI CYCGRG 2 cut(s) 698, 1025
BsiSI CCGG 5 cut(s) 397, 978, 1427, 1468, 1480
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 8 cut(s) 73, 220, 384, 513, 643, 685, 1207, 1632
BsmAI GTCTC 1 cut(s) 900
BsmFI GGGAC 1 cut(s) 413
BsmI GAATGC 1 cut(s) 1146
BsnI GGCC 7 cut(s) 608, 666, 821, 951, 987, 1031, 1426
Bso31I GGTCTC 1 cut(s) 900
BsoBI CYCGRG 2 cut(s) 698, 1025
Bsp1286I GDGCHC 3 cut(s) 734, 1026, 1189
Bsp143I GATC 5 cut(s) 84, 121, 924, 1456, 1542
Bsp68I TCGCGA 1 cut(s) 567
BspACI CCGC 9 cut(s) 195, 231, 315, 458, 585, 676, 695, 723, 956
BspANI GGCC 7 cut(s) 608, 666, 821, 951, 987, 1031, 1426
BspCNI CTCAG 6 cut(s) 45, 64, 149, 158, 357, 589
BspDI ATCGAT 1 cut(s) 464
BspFNI CGCG 5 cut(s) 567, 587, 725, 1365, 1367
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 5 cut(s) 401, 428, 721, 1425, 1466
BspMAI CTGCAG 1 cut(s) 1206
BspMI ACCTGC 1 cut(s) 1101
BspQI GCTCTTC 2 cut(s) 634, 1014
BspT107I GGYRCC 2 cut(s) 426, 1464
BspTNI GGTCTC 1 cut(s) 900
BsrBI CCGCTC 1 cut(s) 697
BsrDI GCAATG 1 cut(s) 767
BsrFI RCCGGY 2 cut(s) 977, 1426
BsrI ACTGG 1 cut(s) 910
BssAI RCCGGY 2 cut(s) 977, 1426
BssECI CCNNGG 7 cut(s) 383, 477, 615, 661, 747, 877, 889
BssHII GCGCGC 1 cut(s) 1363
BssMI GATC 5 cut(s) 84, 121, 924, 1456, 1542
BssNI GRCGYC 1 cut(s) 1465
BssSI CACGAG 2 cut(s) 1357, 1445
BssT1I CCWWGG 6 cut(s) 383, 615, 661, 747, 877, 889
Bst2BI CACGAG 2 cut(s) 1357, 1445
Bst2UI CCWGG 2 cut(s) 1058, 1589
Bst4CI ACNGT 2 cut(s) 293, 995
Bst6I CTCTTC 5 cut(s) 483, 634, 756, 1014, 1569
BstACI GRCGYC 1 cut(s) 1465
BstC8I GCNNGC 5 cut(s) 953, 1365, 1428, 1515, 1521
BstDEI CTNAG 8 cut(s) 53, 72, 136, 145, 344, 597, 968, 1608
BstENI CCTNNNNNAGG 2 cut(s) 71, 382
BstF5I GGATG 4 cut(s) 229, 522, 888, 1479
BstFNI CGCG 5 cut(s) 567, 587, 725, 1365, 1367
BstH2I RGCGCY 1 cut(s) 1468
BstHHI GCGC 6 cut(s) 774, 1365, 1367, 1467, 1501, 1519
BstKTI GATC 5 cut(s) 87, 124, 927, 1459, 1545
BstMAI GTCTC 1 cut(s) 900
BstMBI GATC 5 cut(s) 84, 121, 924, 1456, 1542
BstMWI GCNNNNNNNGC 8 cut(s) 146, 348, 357, 366, 440, 529, 1525, 1638
BstNI CCWGG 2 cut(s) 1058, 1589
BstNSI RCATGY 1 cut(s) 581
BstSCI CCNGG 4 cut(s) 396, 1056, 1479, 1587
BstSFI CTRYAG 1 cut(s) 1202
BstUI CGCG 5 cut(s) 567, 587, 725, 1365, 1367
BstV1I GCAGC 8 cut(s) 127, 369, 446, 467, 1329, 1403, 1483, 1609
BstXI CCANNNNNNTGG 1 cut(s) 616
Bsu15I ATCGAT 1 cut(s) 464
Bsu36I CCTNAGG 1 cut(s) 72
BsuRI GGCC 7 cut(s) 608, 666, 821, 951, 987, 1031, 1426
BsuTUI ATCGAT 1 cut(s) 464
BtgZI GCGATG 1 cut(s) 740
BtsCI GGATG 4 cut(s) 229, 522, 888, 1479
BtsI GCAGTG 2 cut(s) 1298, 1535
BtsIMutI CAGTG 5 cut(s) 50, 917, 1298, 1466, 1535
BtuMI TCGCGA 1 cut(s) 567
BveI ACCTGC 1 cut(s) 1101
Cac8I GCNNGC 5 cut(s) 953, 1365, 1428, 1515, 1521
CciI TCATGA 1 cut(s) 91
CfoI GCGC 6 cut(s) 774, 1365, 1367, 1467, 1501, 1519
Cfr10I RCCGGY 2 cut(s) 977, 1426
Cfr13I GGNCC 7 cut(s) 400, 411, 606, 720, 1210, 1268, 1424
ClaI ATCGAT 1 cut(s) 464
CseI GACGC 1 cut(s) 1085
Csp6I GTAC 3 cut(s) 79, 198, 427
CspAI ACCGGT 1 cut(s) 977
CviAII CATG 9 cut(s) 82, 92, 151, 578, 651, 866, 902, 1214, 1339
CviQI GTAC 3 cut(s) 79, 198, 427
DdeI CTNAG 8 cut(s) 53, 72, 136, 145, 344, 597, 968, 1608
DinI GGCGCC 1 cut(s) 1466
DpnI GATC 5 cut(s) 86, 123, 926, 1458, 1544
DpnII GATC 5 cut(s) 84, 121, 924, 1456, 1542
DraIII CACNNNGTG 1 cut(s) 817
DriI GACNNNNNGTC 2 cut(s) 277, 891
EaeI YGGCCR 1 cut(s) 819
Eam1104I CTCTTC 5 cut(s) 483, 634, 756, 1014, 1569
Eam1105I GACNNNNNGTC 2 cut(s) 277, 891
EarI CTCTTC 5 cut(s) 483, 634, 756, 1014, 1569
Ecl136II GAGCTC 2 cut(s) 1024, 1187
Eco130I CCWWGG 6 cut(s) 383, 615, 661, 747, 877, 889
Eco147I AGGCCT 2 cut(s) 666, 1031
Eco24I GRGCYC 2 cut(s) 1026, 1189
Eco31I GGTCTC 1 cut(s) 900
Eco47I GGWCC 5 cut(s) 400, 411, 720, 1210, 1268
Eco53kI GAGCTC 2 cut(s) 1024, 1187
Eco57I CTGAAG 3 cut(s) 459, 570, 1136
Eco81I CCTNAGG 1 cut(s) 72
Eco88I CYCGRG 2 cut(s) 698, 1025
EcoICRI GAGCTC 2 cut(s) 1024, 1187
EcoNI CCTNNNNNAGG 2 cut(s) 71, 382
EcoRII CCWGG 2 cut(s) 1056, 1587
EcoT14I CCWWGG 6 cut(s) 383, 615, 661, 747, 877, 889
EcoT38I GRGCYC 2 cut(s) 1026, 1189
EgeI GGCGCC 1 cut(s) 1466
EheI GGCGCC 1 cut(s) 1466
ErhI CCWWGG 6 cut(s) 383, 615, 661, 747, 877, 889
FaeI CATG 9 cut(s) 85, 95, 154, 581, 654, 869, 905, 1217, 1342
FaqI GGGAC 1 cut(s) 413
FatI CATG 9 cut(s) 81, 91, 150, 577, 650, 865, 901, 1213, 1338
FbaI TGATCA 2 cut(s) 84, 1456
FblI GTMKAC 2 cut(s) 873, 895
FokI GGATG 4 cut(s) 236, 529, 895, 1486
FriOI GRGCYC 2 cut(s) 1026, 1189
FspBI CTAG 2 cut(s) 680, 1331
GlaI GCGC 6 cut(s) 773, 1364, 1366, 1466, 1500, 1518
GsuI CTGGAG 2 cut(s) 206, 1079
HaeII RGCGCY 1 cut(s) 1468
HaeIII GGCC 7 cut(s) 608, 666, 821, 951, 987, 1031, 1426
HapII CCGG 5 cut(s) 397, 978, 1427, 1468, 1480
HgaI GACGC 1 cut(s) 1085
HhaI GCGC 6 cut(s) 774, 1365, 1367, 1467, 1501, 1519
Hin1I GRCGYC 1 cut(s) 1465
Hin1II CATG 9 cut(s) 85, 95, 154, 581, 654, 869, 905, 1217, 1342
Hin6I GCGC 6 cut(s) 772, 1363, 1365, 1465, 1499, 1517
HinP1I GCGC 6 cut(s) 772, 1363, 1365, 1465, 1499, 1517
HinfI GANTC 3 cut(s) 272, 1253, 1325
HpaII CCGG 5 cut(s) 397, 978, 1427, 1468, 1480
HphI GGTGA 4 cut(s) 1007, 1136, 1180, 1403
Hpy166II GTNNAC 2 cut(s) 874, 896
Hpy188I TCNGA 4 cut(s) 223, 271, 598, 1101
Hpy188III TCNNGA 4 cut(s) 41, 92, 566, 928
Hpy8I GTNNAC 2 cut(s) 874, 896
HpyAV CCTTC 4 cut(s) 23, 751, 1096, 1573
HpyCH4III ACNGT 2 cut(s) 293, 995
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 8 cut(s) 14, 327, 434, 1006, 1159, 1204, 1303, 1316
HpyF10VI GCNNNNNNNGC 8 cut(s) 146, 348, 357, 366, 440, 529, 1525, 1638
HpyF3I CTNAG 8 cut(s) 53, 72, 136, 145, 344, 597, 968, 1608
HpySE526I ACGT 1 cut(s) 36
Hsp92I GRCGYC 1 cut(s) 1465
Hsp92II CATG 9 cut(s) 85, 95, 154, 581, 654, 869, 905, 1217, 1342
HspAI GCGC 6 cut(s) 772, 1363, 1365, 1465, 1499, 1517
KasI GGCGCC 1 cut(s) 1464
KpnI GGTACC 1 cut(s) 430
KroI GCCGGC 1 cut(s) 1426
KroNI GCCGGC 1 cut(s) 1428
Ksp22I TGATCA 2 cut(s) 84, 1456
Kzo9I GATC 5 cut(s) 84, 121, 924, 1456, 1542
LguI GCTCTTC 2 cut(s) 634, 1014
LmnI GCTCC 4 cut(s) 537, 1184, 1246, 1375
Lsp1109I GCAGC 8 cut(s) 127, 369, 446, 467, 1329, 1403, 1483, 1609
LweI GCATC 4 cut(s) 470, 656, 1324, 1500
MaeI CTAG 2 cut(s) 680, 1331
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 6 cut(s) 32, 573, 588, 809, 995, 1559
MalI GATC 5 cut(s) 86, 123, 926, 1458, 1544
MbiI CCGCTC 1 cut(s) 697
MboI GATC 5 cut(s) 84, 121, 924, 1456, 1542
MboII GAAGA 8 cut(s) 500, 621, 773, 1031, 1142, 1381, 1552, 1586
MhlI GDGCHC 3 cut(s) 734, 1026, 1189
MlsI TGGCCA 1 cut(s) 821
MluCI AATT 3 cut(s) 537, 823, 1192
MluNI TGGCCA 1 cut(s) 821
Mly113I GGCGCC 1 cut(s) 1465
MlyI GAGTC 3 cut(s) 266, 1247, 1319
MmeI TCCRAC 2 cut(s) 294, 1580
Mox20I TGGCCA 1 cut(s) 821
MroNI GCCGGC 1 cut(s) 1426
MscI TGGCCA 1 cut(s) 821
MseI TTAA 3 cut(s) 1110, 1401, 1506
Msp20I TGGCCA 1 cut(s) 821
MspA1I CMGCKG 4 cut(s) 140, 437, 458, 1416
MspI CCGG 5 cut(s) 397, 978, 1427, 1468, 1480
MspR9I CCNGG 4 cut(s) 398, 1058, 1481, 1589
Mva1269I GAATGC 1 cut(s) 1146
MvaI CCWGG 2 cut(s) 1058, 1589
MvnI CGCG 5 cut(s) 567, 587, 725, 1365, 1367
MwoI GCNNNNNNNGC 8 cut(s) 146, 348, 357, 366, 440, 529, 1525, 1638
NaeI GCCGGC 1 cut(s) 1428
NarI GGCGCC 1 cut(s) 1465
NciI CCSGG 2 cut(s) 398, 1481
NdeII GATC 5 cut(s) 84, 121, 924, 1456, 1542
NgoMIV GCCGGC 1 cut(s) 1426
NlaIII CATG 9 cut(s) 85, 95, 154, 581, 654, 869, 905, 1217, 1342
NlaIV GGNNCC 5 cut(s) 401, 428, 721, 1425, 1466
NmeAIII GCCGAG 1 cut(s) 502
NmuCI GTSAC 3 cut(s) 573, 809, 995
NruI TCGCGA 1 cut(s) 567
NspI RCATGY 1 cut(s) 581
PaeR7I CTCGAG 1 cut(s) 1025
PagI TCATGA 1 cut(s) 91
PauI GCGCGC 1 cut(s) 1363
PceI AGGCCT 2 cut(s) 666, 1031
PciSI GCTCTTC 2 cut(s) 634, 1014
PcsI WCGNNNNNNNCGW 1 cut(s) 1364
PctI GAATGC 1 cut(s) 1146
PdiI GCCGGC 1 cut(s) 1428
PflMI CCANNNNNTGG 1 cut(s) 643
PfoI TCCNGGA 1 cut(s) 396
PinAI ACCGGT 1 cut(s) 977
PleI GAGTC 3 cut(s) 266, 1247, 1319
PluTI GGCGCC 1 cut(s) 1468
PpsI GAGTC 3 cut(s) 266, 1247, 1319
Psp124BI GAGCTC 2 cut(s) 1026, 1189
Psp6I CCWGG 2 cut(s) 1056, 1587
PspGI CCWGG 2 cut(s) 1056, 1587
PspN4I GGNNCC 5 cut(s) 401, 428, 721, 1425, 1466
PspPI GGNCC 7 cut(s) 400, 411, 606, 720, 1210, 1268, 1424
PspXI VCTCGAGB 1 cut(s) 1025
PstI CTGCAG 1 cut(s) 1206
PteI GCGCGC 1 cut(s) 1363
PvuII CAGCTG 3 cut(s) 140, 437, 1416
RruI TCGCGA 1 cut(s) 567
RsaI GTAC 3 cut(s) 80, 199, 428
RsaNI GTAC 3 cut(s) 79, 198, 427
SacI GAGCTC 2 cut(s) 1026, 1189
SapI GCTCTTC 2 cut(s) 634, 1014
SaqAI TTAA 3 cut(s) 1110, 1401, 1506
Sau3AI GATC 5 cut(s) 84, 121, 924, 1456, 1542
Sau96I GGNCC 7 cut(s) 400, 411, 606, 720, 1210, 1268, 1424
SchI GAGTC 3 cut(s) 266, 1247, 1319
ScrFI CCNGG 4 cut(s) 398, 1058, 1481, 1589
SduI GDGCHC 3 cut(s) 734, 1026, 1189
SfaNI GCATC 4 cut(s) 470, 656, 1324, 1500
SfcI CTRYAG 1 cut(s) 1202
SfoI GGCGCC 1 cut(s) 1466
Sfr274I CTCGAG 1 cut(s) 1025
SinI GGWCC 5 cut(s) 400, 411, 720, 1210, 1268
SlaI CTCGAG 1 cut(s) 1025
SmlI CTYRAG 2 cut(s) 1025, 1255
SmoI CTYRAG 2 cut(s) 1025, 1255
Sse9I AATT 3 cut(s) 537, 823, 1192
SseBI AGGCCT 2 cut(s) 666, 1031
SsiI CCGC 9 cut(s) 195, 231, 315, 458, 585, 676, 695, 723, 956
SspDI GGCGCC 1 cut(s) 1464
SspI AATATT 1 cut(s) 834
SspMI CTAG 2 cut(s) 680, 1331
SstI GAGCTC 2 cut(s) 1026, 1189
StuI AGGCCT 2 cut(s) 666, 1031
StyD4I CCNGG 4 cut(s) 396, 1056, 1479, 1587
StyI CCWWGG 6 cut(s) 383, 615, 661, 747, 877, 889
TaaI ACNGT 2 cut(s) 293, 995
TaiI ACGT 1 cut(s) 39
TaqI TCGA 4 cut(s) 40, 464, 927, 1026
TasI AATT 3 cut(s) 537, 823, 1192
TatI WGTACW 1 cut(s) 78
TauI GCSGC 2 cut(s) 461, 958
Tru1I TTAA 3 cut(s) 1110, 1401, 1506
Tru9I TTAA 3 cut(s) 1110, 1401, 1506
TscAI CASTG 5 cut(s) 57, 917, 1305, 1466, 1535
TseFI GTSAC 3 cut(s) 573, 809, 995
TseI GCWGC 8 cut(s) 140, 357, 434, 455, 1342, 1416, 1496, 1622
Tsp45I GTSAC 3 cut(s) 573, 809, 995
TspDTI ATGAA 8 cut(s) 108, 240, 380, 501, 621, 703, 816, 854
TspRI CASTG 5 cut(s) 57, 917, 1305, 1466, 1535
Van91I CCANNNNNTGG 1 cut(s) 643
VpaK11BI GGWCC 5 cut(s) 400, 411, 720, 1210, 1268
XagI CCTNNNNNAGG 2 cut(s) 71, 382
XceI RCATGY 1 cut(s) 581
XcmI CCANNNNNNNNNTGG 1 cut(s) 803
XhoI CTCGAG 1 cut(s) 1025
XmiI GTMKAC 2 cut(s) 873, 895
XspI CTAG 2 cut(s) 680, 1331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.