Rh1DG013400

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0014738
Physical Location & Seq
Forward (+)
2167131 .. 2167895
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG013400.1

Sequence Viewer

Length: 765 bp
ATGCATCTGCTTTACTATCCGAGCTGTGTGCCAAACGCCAATGCTTTGGTGTTTGGCTCTTCGTTCCAGCGCGTGACGTCTTGCTTTGTCCAAGGCCTAGCCAGCCGGCTAGCCTTGGTTCAACCGCTCGAGGCAGTTGGTTTTGTCCCATCAAAATTGAATGCAAAAGATTATGCTCTAATGGAAAAGAACGAAGAGGCATTGCGCCTTGTTTATGAAATTTGCCCACATATTCAATTTGGTCATTTTGTGGCCAATGCATCAATATTGGAAGCCCTTGAGGGAGAGAGTTTTGTTCACGTGGTAGACCTTGGGATGACCCTCGGTCTGCCACATGGCGATCAGTGGCGAGGCCTGATCCAAAGCCTCGCCACACACGCAGGACAGCCACCAGTGTCACGCCTTCGGATAACTGGTGTTGGTCTGTGCGCTGACAGATTGCTAATTATAGGAGAAGAGCTTGAGGCTTATGCTGACAGCTTAGGAATAAATCTGGAGTTTTCTTGTGTAGAAAGCAACTTGGAAAACTTGAAGCCTGAAGACTTCAAAAATTTTGAGGGTGAAGTACTAGTTGTCAATAGCATTCTTCAGTTGCACTGTGTGGTCAAAGAAAGCAGGGGAGCTCTCAATTCAGTTCTGCAGATGATTCATGAGCTCTCGCCAAAAATCCTAGTTCTTGTTGAGCAGGACTCTAGCCACAATGGATCCCTTTTTTTTTTTTGGAAGGTTTATGGAAGCATTGCATTACTACTCTGCAATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

254

Amino Acids

27.97

Weight (kDa)

5.22

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 15 - 239 2.7e-53 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014738)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g07610
malus_domestica MD06G1148900.v1.1 MD14G1162800.v1.1
prunus_persica Prupe.5G156100_v2.0.a1
pyrus_communis pycom06g13830
rosa_chinensis RchiOBHm_Chr7g0190881
rosa_laevigata RLG00000004489
rosa_multiflora Rmu_sc0004443.1_g000019 Rmu_sc0006903.1_g000003
rosa_roxburghii Rroxscaffold_3G00264230
rosa_rugosa Rorug06G0507700
rosa_samantha Rh1DG013400 Rh1DG013500 Rh7AG114400 Rh7CG119900 Rh7DG118200
rosa_wichuraiana Rw7G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 80
AccBSI CCGCTC 1 cut(s) 127
AccI GTMKAC 1 cut(s) 306
AccII CGCG 1 cut(s) 72
AciI CCGC 1 cut(s) 125
AclWI GGATC 3 cut(s) 352, 699, 712
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 2 cut(s) 219, 550
AcuI CTGAAG 2 cut(s) 558, 572
AcvI CACGTG 1 cut(s) 301
AcyI GRCGYC 1 cut(s) 77
AdeI CACNNNGTG 1 cut(s) 601
AfaI GTAC 1 cut(s) 567
AgsI TTSAA 5 cut(s) 122, 160, 236, 532, 547
AhdI GACNNNNNGTC 1 cut(s) 324
AhlI ACTAGT 1 cut(s) 568
AluBI AGCT 5 cut(s) 24, 460, 480, 623, 655
AluI AGCT 5 cut(s) 24, 460, 480, 623, 655
Alw21I GWGCWC 2 cut(s) 625, 657
AlwI GGATC 3 cut(s) 352, 699, 712
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 3 cut(s) 94, 252, 352
ApoI RAATTY 2 cut(s) 219, 550
AspLEI GCGC 3 cut(s) 72, 207, 431
AsuHPI GGTGA 1 cut(s) 572
AsuNHI GCTAGC 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 128
BalI TGGCCA 1 cut(s) 254
BamHI GGATCC 1 cut(s) 704
BanII GRGCYC 2 cut(s) 625, 657
BbrPI CACGTG 1 cut(s) 301
BbsI GAAGAC 1 cut(s) 546
Bbv12I GWGCWC 2 cut(s) 625, 657
BccI CCATC 1 cut(s) 157
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BcuI ACTAGT 1 cut(s) 568
BfaI CTAG 5 cut(s) 98, 110, 569, 671, 693
BfmI CTRYAG 1 cut(s) 638
BmcAI AGTACT 1 cut(s) 567
BmeRI GACNNNNNGTC 1 cut(s) 324
BmeT110I CYCGRG 1 cut(s) 128
BmiI GGNNCC 1 cut(s) 706
BmsI GCATC 2 cut(s) 13, 269
BmtI GCTAGC 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 546
BplI GAGNNNNNCTC 2 cut(s) 674, 706
BpmI CTGGAG 1 cut(s) 515
Bpu10I CCTNAGC 1 cut(s) 481
BpuEI CTTGAG 2 cut(s) 299, 482
BsaAI YACGTR 1 cut(s) 301
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 4 cut(s) 91, 114, 310, 322
Bse118I RCCGGY 1 cut(s) 105
Bse1I ACTGG 2 cut(s) 392, 418
Bse3DI GCAATG 2 cut(s) 200, 738
BseDI CCNNGG 4 cut(s) 91, 114, 310, 322
BseGI GGATG 1 cut(s) 321
BseMI GCAATG 2 cut(s) 200, 738
BseNI ACTGG 2 cut(s) 392, 418
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 3 cut(s) 96, 254, 354
BsiHKAI GWGCWC 2 cut(s) 625, 657
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 1 cut(s) 106
BslFI GGGAC 1 cut(s) 131
BsmFI GGGAC 1 cut(s) 131
BsmI GAATGC 2 cut(s) 166, 582
BsnI GGCC 3 cut(s) 96, 254, 354
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 625, 657
Bsp143I GATC 3 cut(s) 340, 357, 704
BspACI CCGC 1 cut(s) 125
BspANI GGCC 3 cut(s) 96, 254, 354
BspFNI CGCG 1 cut(s) 72
BspHI TCATGA 1 cut(s) 649
BspLI GGNNCC 1 cut(s) 706
BspMAI CTGCAG 1 cut(s) 642
BspOI GCTAGC 1 cut(s) 113
BspPI GGATC 3 cut(s) 352, 699, 712
BspQI GCTCTTC 2 cut(s) 64, 450
BsrBI CCGCTC 1 cut(s) 127
BsrDI GCAATG 2 cut(s) 200, 738
BsrFI RCCGGY 1 cut(s) 105
BsrI ACTGG 2 cut(s) 392, 418
BssAI RCCGGY 1 cut(s) 105
BssECI CCNNGG 4 cut(s) 91, 114, 310, 322
BssMI GATC 3 cut(s) 340, 357, 704
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 3 cut(s) 91, 114, 310
Bst4CI ACNGT 1 cut(s) 599
Bst6I CTCTTC 3 cut(s) 64, 189, 450
BstACI GRCGYC 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 301
BstC8I GCNNGC 3 cut(s) 103, 107, 111
BstDEI CTNAG 1 cut(s) 481
BstF5I GGATG 1 cut(s) 321
BstFNI CGCG 1 cut(s) 72
BstHHI GCGC 3 cut(s) 72, 207, 431
BstKTI GATC 3 cut(s) 343, 360, 707
BstMBI GATC 3 cut(s) 340, 357, 704
BstMWI GCNNNNNNNGC 2 cut(s) 102, 377
BstSFI CTRYAG 1 cut(s) 638
BstUI CGCG 1 cut(s) 72
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 1 cut(s) 704
BstXI CCANNNNNNTGG 1 cut(s) 46
BstYI RGATCY 1 cut(s) 704
BsuRI GGCC 3 cut(s) 96, 254, 354
BtsCI GGATG 1 cut(s) 321
BtsIMutI CAGTG 3 cut(s) 350, 399, 595
Cac8I GCNNGC 3 cut(s) 103, 107, 111
CciI TCATGA 1 cut(s) 649
CfoI GCGC 3 cut(s) 72, 207, 431
Cfr10I RCCGGY 1 cut(s) 105
Csp6I GTAC 1 cut(s) 566
CviAII CATG 2 cut(s) 335, 650
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 1 cut(s) 481
DpnI GATC 3 cut(s) 342, 359, 706
DpnII GATC 3 cut(s) 340, 357, 704
DraIII CACNNNGTG 1 cut(s) 601
DriI GACNNNNNGTC 1 cut(s) 324
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 3 cut(s) 64, 189, 450
Eam1105I GACNNNNNGTC 1 cut(s) 324
EarI CTCTTC 3 cut(s) 64, 189, 450
Ecl136II GAGCTC 2 cut(s) 623, 655
Eco130I CCWWGG 3 cut(s) 91, 114, 310
Eco147I AGGCCT 2 cut(s) 96, 354
Eco24I GRGCYC 2 cut(s) 625, 657
Eco53kI GAGCTC 2 cut(s) 623, 655
Eco57I CTGAAG 2 cut(s) 558, 572
Eco72I CACGTG 1 cut(s) 301
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 2 cut(s) 623, 655
EcoT14I CCWWGG 3 cut(s) 91, 114, 310
EcoT22I ATGCAT 2 cut(s) 6, 262
EcoT38I GRGCYC 2 cut(s) 625, 657
ErhI CCWWGG 3 cut(s) 91, 114, 310
FaeI CATG 2 cut(s) 338, 653
FaiI YATR 8 cut(s) 174, 216, 231, 336, 449, 471, 651, 732
FaqI GGGAC 1 cut(s) 131
FatI CATG 2 cut(s) 334, 649
FblI GTMKAC 1 cut(s) 306
FokI GGATG 1 cut(s) 328
FriOI GRGCYC 2 cut(s) 625, 657
FspBI CTAG 5 cut(s) 98, 110, 569, 671, 693
GlaI GCGC 3 cut(s) 71, 206, 430
GsuI CTGGAG 1 cut(s) 515
HaeIII GGCC 3 cut(s) 96, 254, 354
HapII CCGG 1 cut(s) 106
HhaI GCGC 3 cut(s) 72, 207, 431
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 2 cut(s) 338, 653
Hin6I GCGC 3 cut(s) 70, 205, 429
HinP1I GCGC 3 cut(s) 70, 205, 429
HinfI GANTC 2 cut(s) 646, 689
HpaII CCGG 1 cut(s) 106
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 2 cut(s) 298, 307
Hpy188I TCNGA 2 cut(s) 21, 408
Hpy188III TCNNGA 2 cut(s) 494, 650
Hpy8I GTNNAC 2 cut(s) 298, 307
HpyAV CCTTC 2 cut(s) 413, 718
HpyCH4III ACNGT 1 cut(s) 599
HpyCH4IV ACGT 2 cut(s) 77, 300
HpyCH4V TGCA 7 cut(s) 4, 164, 260, 595, 640, 743, 756
HpyF10VI GCNNNNNNNGC 2 cut(s) 102, 377
HpyF3I CTNAG 1 cut(s) 481
HpySE526I ACGT 2 cut(s) 77, 300
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 2 cut(s) 338, 653
HspAI GCGC 3 cut(s) 70, 205, 429
KroI GCCGGC 1 cut(s) 105
KroNI GCCGGC 1 cut(s) 107
Kzo9I GATC 3 cut(s) 340, 357, 704
LguI GCTCTTC 2 cut(s) 64, 450
LmnI GCTCC 1 cut(s) 620
LweI GCATC 2 cut(s) 13, 269
MaeI CTAG 5 cut(s) 98, 110, 569, 671, 693
MaeII ACGT 2 cut(s) 77, 300
MaeIII GTNAC 2 cut(s) 73, 396
MalI GATC 3 cut(s) 342, 359, 706
MbiI CCGCTC 1 cut(s) 127
MboI GATC 3 cut(s) 340, 357, 704
MboII GAAGA 5 cut(s) 51, 206, 467, 551, 578
MflI RGATCY 1 cut(s) 704
MhlI GDGCHC 2 cut(s) 625, 657
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 6 cut(s) 155, 219, 236, 444, 550, 628
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 1 cut(s) 683
MnlI CCTC 8 cut(s) 124, 190, 274, 332, 344, 377, 457, 550
Mox20I TGGCCA 1 cut(s) 254
Mph1103I ATGCAT 2 cut(s) 6, 262
MroNI GCCGGC 1 cut(s) 105
MscI TGGCCA 1 cut(s) 254
Msp20I TGGCCA 1 cut(s) 254
MspI CCGG 1 cut(s) 106
Mva1269I GAATGC 2 cut(s) 166, 582
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 2 cut(s) 102, 377
NaeI GCCGGC 1 cut(s) 107
NdeII GATC 3 cut(s) 340, 357, 704
NgoMIV GCCGGC 1 cut(s) 105
NheI GCTAGC 1 cut(s) 109
NlaIII CATG 2 cut(s) 338, 653
NlaIV GGNNCC 1 cut(s) 706
NmuCI GTSAC 2 cut(s) 73, 396
NsiI ATGCAT 2 cut(s) 6, 262
PaeR7I CTCGAG 1 cut(s) 128
PagI TCATGA 1 cut(s) 649
PceI AGGCCT 2 cut(s) 96, 354
PciSI GCTCTTC 2 cut(s) 64, 450
PctI GAATGC 2 cut(s) 166, 582
PdiI GCCGGC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 646
PleI GAGTC 1 cut(s) 683
PmaCI CACGTG 1 cut(s) 301
PmlI CACGTG 1 cut(s) 301
PpsI GAGTC 1 cut(s) 683
Ppu21I YACGTR 1 cut(s) 301
Psp124BI GAGCTC 2 cut(s) 625, 657
PspCI CACGTG 1 cut(s) 301
PspN4I GGNNCC 1 cut(s) 706
PspXI VCTCGAGB 1 cut(s) 128
PstI CTGCAG 1 cut(s) 642
PsuI RGATCY 1 cut(s) 704
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
SacI GAGCTC 2 cut(s) 625, 657
SapI GCTCTTC 2 cut(s) 64, 450
Sau3AI GATC 3 cut(s) 340, 357, 704
ScaI AGTACT 1 cut(s) 567
SchI GAGTC 1 cut(s) 683
SduI GDGCHC 2 cut(s) 625, 657
SetI ASST 9 cut(s) 26, 80, 303, 312, 462, 482, 625, 657, 729
SfaNI GCATC 2 cut(s) 13, 269
SfcI CTRYAG 1 cut(s) 638
Sfr274I CTCGAG 1 cut(s) 128
SlaI CTCGAG 1 cut(s) 128
SmlI CTYRAG 3 cut(s) 128, 278, 461
SmoI CTYRAG 3 cut(s) 128, 278, 461
SpeI ACTAGT 1 cut(s) 568
Sse9I AATT 6 cut(s) 155, 219, 236, 444, 550, 628
SseBI AGGCCT 2 cut(s) 96, 354
SsiI CCGC 1 cut(s) 125
SspI AATATT 2 cut(s) 267, 760
SspMI CTAG 5 cut(s) 98, 110, 569, 671, 693
SstI GAGCTC 2 cut(s) 625, 657
StuI AGGCCT 2 cut(s) 96, 354
StyI CCWWGG 3 cut(s) 91, 114, 310
TaaI ACNGT 1 cut(s) 599
TaiI ACGT 2 cut(s) 80, 303
TaqI TCGA 1 cut(s) 129
TaqII GACCGA 1 cut(s) 314
TasI AATT 6 cut(s) 155, 219, 236, 444, 550, 628
TatI WGTACW 1 cut(s) 565
TfiI GAWTC 1 cut(s) 646
TscAI CASTG 3 cut(s) 350, 399, 602
TseFI GTSAC 2 cut(s) 73, 396
Tsp45I GTSAC 2 cut(s) 73, 396
TspDTI ATGAA 2 cut(s) 231, 638
TspRI CASTG 3 cut(s) 350, 399, 602
XapI RAATTY 2 cut(s) 219, 550
XhoI CTCGAG 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 5 cut(s) 98, 110, 569, 671, 693
ZraI GACGTC 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 567
Zsp2I ATGCAT 2 cut(s) 6, 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.