MD06G1148900.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014738
Physical Location & Seq
Forward (+)
29171471 .. 29173054
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1148900.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGGGGCCAAACCGAAAATTCCTGCCCAAGGAGTACATGATCAATAATGAAAGGACTAATAAAAGGAGTGCCCTTGATACCATTACTCACTCAGCTGCCTCAGCCATGCCTTGCTATCCTTATCCATACACGCCCTTTCTGGAGGAGGGCGCTACAGCTTCTATCCTCCCTTACTCGGACGAAAGCGGAGACCATAAAAAGCTGAAAAGGAATATAAATATCTCCGACAGTCATAACAGCATTTATAGTGGTGGAGGAAGCGAAAATAGTTGCATTACTGACATCAGCCTCAGCCGCAGCAGCAGCACGAATAGCTTCAACACCTTGCGAAGGCTTCATTTCCGGGATCATATTTGGACTTACTCACAAAGGTACCTTGCAGCTGAGGCTGTTGAGGAGGCAGCAACAGCTATGTCCAATGCCGAGGAAAATGGAGCGGAGGAGGACGGAACTGCAGATGGGATGAGGCTTGTTCAGCTCCTGATTGCTTGCGCTGAAGCTGTTGCTTGTCGCGACAAGTCACATGCCTCTGCGTTACTATCTGAGCTTCGGGCCAATGCTTTGGTCTTTGGTTCTTCATTCCAGCGCGTGGCATCGTGTTTTGTCCAAGGCCTTGCCAACCGCCTAGCCCTGGTTCAACCGCTCGGGGCAGTAGGGTTCATTGGGTCCGCAATGAGCACGAAAGACTTTGACAAGAAGGAAGAGGCATTGCGCCTTGTTTATGAGATTTGCCCCCACATTCAGTTTGGTCACTTTGTGGCCAATTCATCAATATTGGAAGCCTTTGAGGGAGAGAGTTTTGTTCATGTGGTAGACCTTGGGATGACCCTTGGTCTACCACATGGAGACCAGTGGCGAGGCTTGATCCAAAGCCTTGCCACACGCACAGGCCGGCCGCCTACACGCCTCAGAATAACTGGGGTTGGCCTTTGTGGTGACAAATTGCAAATCATTGGAGGAGAGCTCGAGGCCTACGCCGAAAGCTTGGGAATAAACCTGGAGTTTTCTGTGGTGGAAAGCAATTTGGAAAACCTGCGTCCGAAGGACATCAAAATGTTTGATGGTGAAGTCCTTGTTGTCAATAGCATTCTTCAGTTGCATTGTGTGGTGAAAGAAAGCAGAGGAGCTCTCAATTCTGTCCTGCAGATGATCCATGAGCTTTTGCACAAAATCTTTGTTCTTGTGGAGCAAGACTCAAGCCACAATGGACCATTTTTTTTGGGGAGGTTTATGGAAGCACTGCATTATTACTCTGCAATCTTTGACTCCCTCGATACCATGCTGCCAAAATATGACACGAGGCGCGCGAAGATGGAGCAGTTCTACTTTGCTGAGGAGATTAAGAACATAATCAGCTGTGAGGGGCCGGCAAGGGTGGAGAGGCACGAGAGGGTTGATCAGTGGCGTCGGAGGATGAGCCGTGCTGGGTTTCAGGCTACGCCGATTAAGATGCTGGCGCAGGCAAAGCAGTGGCTCGGAAAGATCAAGGTCTGTGAAGGCTACACTATCATGGAAGAGAAGGGTTGTTTAGTTCTTGGTTGGCAATCCAAGCCCATTGTTGCTGCCTCCTGCTGGAAATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

528

Amino Acids

58.72

Weight (kDa)

6.37

Isoelectric Point (pI)

54.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 157 - 526 7.9e-111 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014738)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g07610
malus_domestica MD06G1148900.v1.1 MD14G1162800.v1.1
prunus_persica Prupe.5G156100_v2.0.a1
pyrus_communis pycom06g13830
rosa_chinensis RchiOBHm_Chr7g0190881
rosa_laevigata RLG00000004489
rosa_multiflora Rmu_sc0004443.1_g000019 Rmu_sc0006903.1_g000003
rosa_roxburghii Rroxscaffold_3G00264230
rosa_rugosa Rorug06G0507700
rosa_samantha Rh1DG013400 Rh1DG013500 Rh7AG114400 Rh7CG119900 Rh7DG118200
rosa_wichuraiana Rw7G009980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1041
Acc65I GGTACC 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 372
AccB7I CCANNNNNTGG 1 cut(s) 589
AccBSI CCGCTC 2 cut(s) 437, 643
AccI GTMKAC 2 cut(s) 813, 835
AccII CGCG 4 cut(s) 513, 588, 1305, 1307
AciI CCGC 7 cut(s) 186, 295, 437, 622, 641, 669, 896
AclWI GGATC 3 cut(s) 354, 859, 1144
AcoI YGGCCR 2 cut(s) 759, 893
AcsI RAATTY 1 cut(s) 17
AcuI CTGAAG 2 cut(s) 516, 1076
AcyI GRCGYC 1 cut(s) 1405
AdeI CACNNNGTG 1 cut(s) 757
AfaI GTAC 2 cut(s) 35, 374
AfiI CCNNNNNNNGG 6 cut(s) 28, 175, 330, 589, 631, 1572
AgsI TTSAA 2 cut(s) 319, 638
AhdI GACNNNNNGTC 1 cut(s) 831
AjnI CCWGG 2 cut(s) 630, 996
Alw21I GWGCWC 3 cut(s) 680, 966, 1129
Alw26I GTCTC 2 cut(s) 183, 840
AlwI GGATC 3 cut(s) 354, 859, 1144
AlwNI CAGNNNCTG 1 cut(s) 481
Ama87I CYCGRG 2 cut(s) 644, 965
AoxI GGCC 9 cut(s) 5, 552, 610, 759, 889, 893, 925, 969, 1364
ApeKI GCWGC 8 cut(s) 95, 297, 300, 303, 380, 401, 1282, 1562
ApoI RAATTY 1 cut(s) 17
Asp700I GAANNNNTTC 1 cut(s) 314
Asp718I GGTACC 1 cut(s) 372
AspLEI GCGC 7 cut(s) 152, 494, 588, 714, 1305, 1307, 1459
AspS9I GGNCC 5 cut(s) 5, 552, 666, 1208, 1364
AsuC2I CCSGG 1 cut(s) 344
AsuHPI GGTGA 3 cut(s) 947, 1076, 1120
AvaI CYCGRG 2 cut(s) 644, 965
AvaII GGWCC 2 cut(s) 666, 1208
BaeGI GKGCMC 1 cut(s) 73
BaeI ACNNNNGTAYC 2 cut(s) 356, 389
BalI TGGCCA 1 cut(s) 761
BanI GGYRCC 1 cut(s) 372
BanII GRGCYC 2 cut(s) 966, 1129
BauI CACGAG 2 cut(s) 1297, 1385
Bbv12I GWGCWC 3 cut(s) 680, 966, 1129
BbvCI CCTCAGC 4 cut(s) 100, 290, 384, 1332
BbvI GCAGC 8 cut(s) 82, 309, 312, 315, 392, 413, 1269, 1549
BccI CCATC 3 cut(s) 452, 1055, 1306
BceAI ACGGC 1 cut(s) 1404
BcgI CGANNNNNNTGC 2 cut(s) 1432, 1466
BciT130I CCWGG 2 cut(s) 632, 998
BclI TGATCA 2 cut(s) 39, 1396
BcnI CCSGG 1 cut(s) 344
BcoDI GTCTC 2 cut(s) 183, 840
BfaI CTAG 1 cut(s) 626
BfmI CTRYAG 3 cut(s) 153, 453, 1142
BfoI RGCGCY 1 cut(s) 153
BfuAI ACCTGC 1 cut(s) 1041
Bme1390I CCNGG 3 cut(s) 344, 632, 998
Bme18I GGWCC 2 cut(s) 666, 1208
BmeRI GACNNNNNGTC 1 cut(s) 831
BmeT110I CYCGRG 2 cut(s) 644, 965
BmgT120I GGNCC 5 cut(s) 5, 552, 666, 1208, 1364
BmiI GGNNCC 4 cut(s) 6, 374, 667, 1365
BmrFI CCNGG 3 cut(s) 344, 632, 998
BmrI ACTGGG 1 cut(s) 927
BmsI GCATC 2 cut(s) 602, 1440
BmuI ACTGGG 1 cut(s) 927
BplI GAGNNNNNCTC 6 cut(s) 948, 980, 1113, 1145, 1178, 1210
BpmI CTGGAG 2 cut(s) 161, 1019
Bpu10I CCTNAGC 4 cut(s) 100, 290, 384, 1332
BpuEI CTTGAG 1 cut(s) 1180
BpuMI CCSGG 1 cut(s) 344
BsaHI GRCGYC 1 cut(s) 1405
BsaI GGTCTC 2 cut(s) 183, 840
BsaJI CCNNGG 6 cut(s) 27, 423, 607, 630, 817, 829
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 175, 330, 589, 631, 1572
Bse118I RCCGGY 2 cut(s) 891, 1366
Bse1I ACTGG 2 cut(s) 850, 922
Bse3DI GCAATG 2 cut(s) 678, 707
BseBI CCWGG 2 cut(s) 632, 998
BseDI CCNNGG 6 cut(s) 27, 423, 607, 630, 817, 829
BseGI GGATG 3 cut(s) 468, 828, 1419
BseLI CCNNNNNNNGG 6 cut(s) 28, 175, 330, 589, 631, 1572
BseMI GCAATG 2 cut(s) 678, 707
BseMII CTCAG 7 cut(s) 105, 114, 304, 375, 534, 922, 1323
BseNI ACTGG 2 cut(s) 850, 922
BsePI GCGCGC 1 cut(s) 1303
BseRI GAGGAG 6 cut(s) 158, 410, 455, 972, 1137, 1349
BseSI GKGCMC 1 cut(s) 73
BseX3I CGGCCG 1 cut(s) 893
BseXI GCAGC 8 cut(s) 82, 309, 312, 315, 392, 413, 1269, 1549
BseYI CCCAGC 1 cut(s) 1424
Bsh1236I CGCG 4 cut(s) 513, 588, 1305, 1307
Bsh1285I CGRYCG 1 cut(s) 896
BshFI GGCC 9 cut(s) 7, 554, 612, 761, 891, 895, 927, 971, 1366
BshNI GGYRCC 1 cut(s) 372
BsiEI CGRYCG 1 cut(s) 896
BsiHKAI GWGCWC 3 cut(s) 680, 966, 1129
BsiHKCI CYCGRG 2 cut(s) 644, 965
BsiSI CCGG 3 cut(s) 343, 892, 1367
BslI CCNNNNNNNGG 6 cut(s) 28, 175, 330, 589, 631, 1572
BsmAI GTCTC 2 cut(s) 183, 840
BsmI GAATGC 1 cut(s) 1086
BsnI GGCC 9 cut(s) 7, 554, 612, 761, 891, 895, 927, 971, 1366
Bso31I GGTCTC 2 cut(s) 183, 840
BsoBI CYCGRG 2 cut(s) 644, 965
Bsp1286I GDGCHC 4 cut(s) 73, 680, 966, 1129
Bsp143I GATC 6 cut(s) 39, 346, 864, 1149, 1396, 1482
Bsp68I TCGCGA 1 cut(s) 513
BspACI CCGC 7 cut(s) 186, 295, 437, 622, 641, 669, 896
BspANI GGCC 9 cut(s) 7, 554, 612, 761, 891, 895, 927, 971, 1366
BspCNI CTCAG 7 cut(s) 104, 113, 303, 376, 535, 921, 1324
BspFNI CGCG 4 cut(s) 513, 588, 1305, 1307
BspLI GGNNCC 4 cut(s) 6, 374, 667, 1365
BspMAI CTGCAG 2 cut(s) 457, 1146
BspMI ACCTGC 1 cut(s) 1041
BspPI GGATC 3 cut(s) 354, 859, 1144
BspT107I GGYRCC 1 cut(s) 372
BspTNI GGTCTC 2 cut(s) 183, 840
BsrBI CCGCTC 2 cut(s) 437, 643
BsrDI GCAATG 2 cut(s) 678, 707
BsrFI RCCGGY 2 cut(s) 891, 1366
BsrI ACTGG 2 cut(s) 850, 922
BssAI RCCGGY 2 cut(s) 891, 1366
BssECI CCNNGG 6 cut(s) 27, 423, 607, 630, 817, 829
BssHII GCGCGC 1 cut(s) 1303
BssMI GATC 6 cut(s) 39, 346, 864, 1149, 1396, 1482
BssNI GRCGYC 1 cut(s) 1405
BssSI CACGAG 2 cut(s) 1297, 1385
BssT1I CCWWGG 4 cut(s) 27, 607, 817, 829
Bst2BI CACGAG 2 cut(s) 1297, 1385
Bst2UI CCWGG 2 cut(s) 632, 998
Bst4CI ACNGT 1 cut(s) 230
Bst6I CTCTTC 2 cut(s) 696, 1509
BstACI GRCGYC 1 cut(s) 1405
BstC8I GCNNGC 6 cut(s) 490, 893, 1305, 1368, 1455, 1461
BstDEI CTNAG 7 cut(s) 91, 100, 290, 384, 543, 908, 1332
BstENI CCTNNNNNAGG 2 cut(s) 26, 328
BstF5I GGATG 3 cut(s) 468, 828, 1419
BstFNI CGCG 4 cut(s) 513, 588, 1305, 1307
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 7 cut(s) 152, 494, 588, 714, 1305, 1307, 1459
BstKTI GATC 6 cut(s) 42, 349, 867, 1152, 1399, 1485
BstMAI GTCTC 2 cut(s) 183, 840
BstMBI GATC 6 cut(s) 39, 346, 864, 1149, 1396, 1482
BstMCI CGRYCG 1 cut(s) 896
BstNI CCWGG 2 cut(s) 632, 998
BstNSI RCATGY 1 cut(s) 527
BstSCI CCNGG 3 cut(s) 342, 630, 996
BstSFI CTRYAG 3 cut(s) 153, 453, 1142
BstSLI GKGCMC 1 cut(s) 73
BstUI CGCG 4 cut(s) 513, 588, 1305, 1307
BstV1I GCAGC 8 cut(s) 82, 309, 312, 315, 392, 413, 1269, 1549
BstXI CCANNNNNNTGG 1 cut(s) 562
BstZI CGGCCG 1 cut(s) 893
BsuRI GGCC 9 cut(s) 7, 554, 612, 761, 891, 895, 927, 971, 1366
BtsCI GGATG 3 cut(s) 468, 828, 1419
BtsI GCAGTG 2 cut(s) 1238, 1475
BtsIMutI CAGTG 4 cut(s) 857, 1238, 1406, 1475
BtuMI TCGCGA 1 cut(s) 513
BveI ACCTGC 1 cut(s) 1041
Cac8I GCNNGC 6 cut(s) 490, 893, 1305, 1368, 1455, 1461
CaiI CAGNNNCTG 1 cut(s) 481
CfoI GCGC 7 cut(s) 152, 494, 588, 714, 1305, 1307, 1459
Cfr10I RCCGGY 2 cut(s) 891, 1366
Cfr13I GGNCC 5 cut(s) 5, 552, 666, 1208, 1364
CseI GACGC 2 cut(s) 1025, 1394
Csp6I GTAC 2 cut(s) 34, 373
CviAII CATG 8 cut(s) 37, 106, 524, 806, 842, 1154, 1279, 1510
CviQI GTAC 2 cut(s) 34, 373
DdeI CTNAG 7 cut(s) 91, 100, 290, 384, 543, 908, 1332
DpnI GATC 6 cut(s) 41, 348, 866, 1151, 1398, 1484
DpnII GATC 6 cut(s) 39, 346, 864, 1149, 1396, 1482
DraIII CACNNNGTG 1 cut(s) 757
DriI GACNNNNNGTC 1 cut(s) 831
EaeI YGGCCR 2 cut(s) 759, 893
EagI CGGCCG 1 cut(s) 893
Eam1104I CTCTTC 2 cut(s) 696, 1509
Eam1105I GACNNNNNGTC 1 cut(s) 831
EarI CTCTTC 2 cut(s) 696, 1509
Ecl136II GAGCTC 2 cut(s) 964, 1127
EclXI CGGCCG 1 cut(s) 893
Eco130I CCWWGG 4 cut(s) 27, 607, 817, 829
Eco147I AGGCCT 2 cut(s) 612, 971
Eco24I GRGCYC 2 cut(s) 966, 1129
Eco31I GGTCTC 2 cut(s) 183, 840
Eco47I GGWCC 2 cut(s) 666, 1208
Eco52I CGGCCG 1 cut(s) 893
Eco53kI GAGCTC 2 cut(s) 964, 1127
Eco57I CTGAAG 2 cut(s) 516, 1076
Eco88I CYCGRG 2 cut(s) 644, 965
EcoICRI GAGCTC 2 cut(s) 964, 1127
EcoNI CCTNNNNNAGG 2 cut(s) 26, 328
EcoRII CCWGG 2 cut(s) 630, 996
EcoT14I CCWWGG 4 cut(s) 27, 607, 817, 829
EcoT38I GRGCYC 2 cut(s) 966, 1129
ErhI CCWWGG 4 cut(s) 27, 607, 817, 829
FaeI CATG 8 cut(s) 40, 109, 527, 809, 845, 1157, 1282, 1513
FatI CATG 8 cut(s) 36, 105, 523, 805, 841, 1153, 1278, 1509
FbaI TGATCA 2 cut(s) 39, 1396
FblI GTMKAC 2 cut(s) 813, 835
FokI GGATG 3 cut(s) 475, 835, 1426
FriOI GRGCYC 2 cut(s) 966, 1129
FseI GGCCGGCC 1 cut(s) 895
FspBI CTAG 1 cut(s) 626
GlaI GCGC 7 cut(s) 151, 493, 587, 713, 1304, 1306, 1458
GsaI CCCAGC 1 cut(s) 1428
GsuI CTGGAG 2 cut(s) 161, 1019
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 9 cut(s) 7, 554, 612, 761, 891, 895, 927, 971, 1366
HapII CCGG 3 cut(s) 343, 892, 1367
HgaI GACGC 2 cut(s) 1025, 1394
HhaI GCGC 7 cut(s) 152, 494, 588, 714, 1305, 1307, 1459
Hin1I GRCGYC 1 cut(s) 1405
Hin1II CATG 8 cut(s) 40, 109, 527, 809, 845, 1157, 1282, 1513
Hin6I GCGC 7 cut(s) 150, 492, 586, 712, 1303, 1305, 1457
HinP1I GCGC 7 cut(s) 150, 492, 586, 712, 1303, 1305, 1457
HindIII AAGCTT 1 cut(s) 982
HinfI GANTC 2 cut(s) 1193, 1265
HpaII CCGG 3 cut(s) 343, 892, 1367
HphI GGTGA 3 cut(s) 947, 1076, 1120
Hpy166II GTNNAC 2 cut(s) 814, 836
Hpy188I TCNGA 7 cut(s) 178, 226, 544, 911, 1041, 1410, 1478
Hpy188III TCNNGA 3 cut(s) 140, 481, 512
Hpy8I GTNNAC 2 cut(s) 814, 836
Hpy99I CGWCG 1 cut(s) 1410
HpyAV CCTTC 5 cut(s) 324, 691, 1036, 1490, 1513
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4V TGCA 9 cut(s) 273, 380, 455, 946, 1099, 1144, 1165, 1243, 1256
HpyF3I CTNAG 7 cut(s) 91, 100, 290, 384, 543, 908, 1332
Hsp92I GRCGYC 1 cut(s) 1405
Hsp92II CATG 8 cut(s) 40, 109, 527, 809, 845, 1157, 1282, 1513
HspAI GCGC 7 cut(s) 150, 492, 586, 712, 1303, 1305, 1457
KpnI GGTACC 1 cut(s) 376
KroI GCCGGC 2 cut(s) 891, 1366
KroNI GCCGGC 2 cut(s) 893, 1368
Ksp22I TGATCA 2 cut(s) 39, 1396
Kzo9I GATC 6 cut(s) 39, 346, 864, 1149, 1396, 1482
LmnI GCTCC 5 cut(s) 434, 483, 1124, 1186, 1315
Lsp1109I GCAGC 8 cut(s) 82, 309, 312, 315, 392, 413, 1269, 1549
LweI GCATC 2 cut(s) 602, 1440
MaeI CTAG 1 cut(s) 626
MaeIII GTNAC 4 cut(s) 519, 534, 749, 935
MalI GATC 6 cut(s) 41, 348, 866, 1151, 1398, 1484
MbiI CCGCTC 2 cut(s) 437, 643
MboI GATC 6 cut(s) 39, 346, 864, 1149, 1396, 1482
MboII GAAGA 5 cut(s) 567, 713, 1082, 1321, 1526
MhlI GDGCHC 4 cut(s) 73, 680, 966, 1129
MlsI TGGCCA 1 cut(s) 761
MluCI AATT 5 cut(s) 17, 763, 941, 1021, 1132
MluNI TGGCCA 1 cut(s) 761
MlyI GAGTC 2 cut(s) 1187, 1259
MmeI TCCRAC 2 cut(s) 249, 1388
Mox20I TGGCCA 1 cut(s) 761
MroNI GCCGGC 2 cut(s) 891, 1366
MroXI GAANNNNTTC 1 cut(s) 314
MscI TGGCCA 1 cut(s) 761
MseI TTAA 2 cut(s) 1341, 1446
MslI CAYNNNNRTG 2 cut(s) 1052, 1508
Msp20I TGGCCA 1 cut(s) 761
MspA1I CMGCKG 3 cut(s) 95, 383, 1356
MspI CCGG 3 cut(s) 343, 892, 1367
MspR9I CCNGG 3 cut(s) 344, 632, 998
Mva1269I GAATGC 1 cut(s) 1086
MvaI CCWGG 2 cut(s) 632, 998
MvnI CGCG 4 cut(s) 513, 588, 1305, 1307
NaeI GCCGGC 2 cut(s) 893, 1368
NciI CCSGG 1 cut(s) 344
NdeII GATC 6 cut(s) 39, 346, 864, 1149, 1396, 1482
NgoMIV GCCGGC 2 cut(s) 891, 1366
NlaIII CATG 8 cut(s) 40, 109, 527, 809, 845, 1157, 1282, 1513
NlaIV GGNNCC 4 cut(s) 6, 374, 667, 1365
NmeAIII GCCGAG 1 cut(s) 448
NmuCI GTSAC 3 cut(s) 519, 749, 935
NruI TCGCGA 1 cut(s) 513
NspI RCATGY 1 cut(s) 527
PaeR7I CTCGAG 1 cut(s) 965
PauI GCGCGC 1 cut(s) 1303
PceI AGGCCT 2 cut(s) 612, 971
PcsI WCGNNNNNNNCGW 1 cut(s) 1304
PctI GAATGC 1 cut(s) 1086
PdiI GCCGGC 2 cut(s) 893, 1368
PdmI GAANNNNTTC 1 cut(s) 314
PflMI CCANNNNNTGG 1 cut(s) 589
PfoI TCCNGGA 1 cut(s) 342
PleI GAGTC 2 cut(s) 1187, 1259
PpsI GAGTC 2 cut(s) 1187, 1259
Psp124BI GAGCTC 2 cut(s) 966, 1129
Psp6I CCWGG 2 cut(s) 630, 996
PspFI CCCAGC 1 cut(s) 1424
PspGI CCWGG 2 cut(s) 630, 996
PspN4I GGNNCC 4 cut(s) 6, 374, 667, 1365
PspPI GGNCC 5 cut(s) 5, 552, 666, 1208, 1364
PspXI VCTCGAGB 1 cut(s) 965
PstI CTGCAG 2 cut(s) 457, 1146
PstNI CAGNNNCTG 1 cut(s) 481
PteI GCGCGC 1 cut(s) 1303
PvuII CAGCTG 3 cut(s) 95, 383, 1356
RigI GGCCGGCC 1 cut(s) 895
RruI TCGCGA 1 cut(s) 513
RsaI GTAC 2 cut(s) 35, 374
RsaNI GTAC 2 cut(s) 34, 373
RseI CAYNNNNRTG 2 cut(s) 1052, 1508
SacI GAGCTC 2 cut(s) 966, 1129
SaqAI TTAA 2 cut(s) 1341, 1446
Sau3AI GATC 6 cut(s) 39, 346, 864, 1149, 1396, 1482
Sau96I GGNCC 5 cut(s) 5, 552, 666, 1208, 1364
SchI GAGTC 2 cut(s) 1187, 1259
ScrFI CCNGG 3 cut(s) 344, 632, 998
SduI GDGCHC 4 cut(s) 73, 680, 966, 1129
SfaNI GCATC 2 cut(s) 602, 1440
SfcI CTRYAG 3 cut(s) 153, 453, 1142
Sfr274I CTCGAG 1 cut(s) 965
SinI GGWCC 2 cut(s) 666, 1208
SlaI CTCGAG 1 cut(s) 965
SmiMI CAYNNNNRTG 2 cut(s) 1052, 1508
SmlI CTYRAG 2 cut(s) 965, 1195
SmoI CTYRAG 2 cut(s) 965, 1195
Sse9I AATT 5 cut(s) 17, 763, 941, 1021, 1132
SseBI AGGCCT 2 cut(s) 612, 971
SsiI CCGC 7 cut(s) 186, 295, 437, 622, 641, 669, 896
SspI AATATT 1 cut(s) 774
SspMI CTAG 1 cut(s) 626
SstI GAGCTC 2 cut(s) 966, 1129
StuI AGGCCT 2 cut(s) 612, 971
StyD4I CCNGG 3 cut(s) 342, 630, 996
StyI CCWWGG 4 cut(s) 27, 607, 817, 829
TaaI ACNGT 1 cut(s) 230
TaqI TCGA 2 cut(s) 966, 1272
TasI AATT 5 cut(s) 17, 763, 941, 1021, 1132
TatI WGTACW 1 cut(s) 33
TauI GCSGC 2 cut(s) 297, 898
Tru1I TTAA 2 cut(s) 1341, 1446
Tru9I TTAA 2 cut(s) 1341, 1446
TscAI CASTG 4 cut(s) 857, 1245, 1406, 1475
TseFI GTSAC 3 cut(s) 519, 749, 935
TseI GCWGC 8 cut(s) 95, 297, 300, 303, 380, 401, 1282, 1562
Tsp45I GTSAC 3 cut(s) 519, 749, 935
TspDTI ATGAA 6 cut(s) 63, 326, 567, 649, 756, 794
TspGWI ACGGA 1 cut(s) 462
TspRI CASTG 4 cut(s) 857, 1245, 1406, 1475
Van91I CCANNNNNTGG 1 cut(s) 589
VpaK11BI GGWCC 2 cut(s) 666, 1208
XagI CCTNNNNNAGG 2 cut(s) 26, 328
XapI RAATTY 1 cut(s) 17
XceI RCATGY 1 cut(s) 527
XcmI CCANNNNNNNNNTGG 1 cut(s) 743
XhoI CTCGAG 1 cut(s) 965
XmiI GTMKAC 2 cut(s) 813, 835
XmnI GAANNNNTTC 1 cut(s) 314
XspI CTAG 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.