FvH4_5g13420

Isoaspartyl peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0017149
Physical Location & Seq
Forward (+)
7581386 .. 7583387
2002 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13420.t1

Sequence Viewer

Length: 966 bp
ATGGGGTGGGCGATCGCGCTCCATGGCGGCGCCGGCGACATCCCCCTCTCTCTTTCGCCGGAGCGCCGCCAGCCTCGCGAGGCCGCCCTCCGCCACTGCCTCCAGATCGGCGTCGAAGCTCTCAAGGCCAACAAGCCTCCTCTCGACGTCGTTGAGCTCGTCGTTCGCGAGCTGGAGAATATTCCGGTGTTCAACGCCGGGAAGGGGTCGGTGTTGACCAGCAACGGCACGGTGGAGATGGAAGCTTGTATTATGGACGGCACCAAGAGATGTGGAGCTGTTTCTGGGCTCACTACTGTGGTCAATCCCATATCTTTAGCCAGATTGGTCATGGAGAACACTCCTCACATCTATCTTGCTTTTGATGGAGCAGAGGCCTTTGCTAGAGAACAAGGTGTTGAGACTGCAGATGCGAATAGCTTCATTACACCGGAGAATGTTGAGAGGCTAAAGCAGGCGATAGAGGCCAACAGGGTGCAGATTGATTATACACAGCCAATTCAGAAAGATGTGAAGGATAAAGCACCTGAAGCTGATGGCGATAGTCAGCTTGGAACTGTTGGATGTGTGGCTGTTGATGATCTTGGGAATTTGGCTTCTGCAACTTCGACAGGCGGATTGGTTAACAAGATGGTTGGCCGGATTGGGGACACACCCATTATCGGTGCAGGGACATATGCCAACAATCTGTGTGCAGTTTCTGCCACTGGCAAAGGTGAAGCAATAATCCGAAGTACAGCTGCGAGGGATGTGGCAGCTCTTATGGAGTACAAAGGTGTCTCTCTTAAAGAGGCTGCAGCTTATGTTGTGGAGGAGAGCACTCCGAAAGGCAATGTTGGCTTGGTTGCTGTATCTGCCACAGGAGAAGTCACAATGCCTTTCAATACAACAGGGATGTTTCGAGCTTGTGCTACTGAGGATGGGTATTGGGAGATTGGAATATGGCCTTCTGATGTGCAAAAATGA

Protein Analysis

322

Amino Acids

33.76

Weight (kDa)

4.84

Isoelectric Point (pI)

30.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 1 - 313 7.1e-114 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08100
fragaria_vesca FvH4_5g13420
malus_domestica MD06G1205300.v1.1 MD06G1205500.v1.1
prunus_persica Prupe.5G209700_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0183691
rosa_laevigata RLG00000005032
rosa_multiflora Rmu_sc0003610.1_g000026
rosa_roxburghii Rroxscaffold_3G00270320
rosa_rugosa Rorug06G0459400
rosa_samantha Rh7BG060300 Rh7DG060200
rosa_wichuraiana Rw7G004990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB1I GGYRCC 2 cut(s) 29, 260
AccII CGCG 3 cut(s) 17, 78, 168
AciI CCGC 5 cut(s) 27, 67, 84, 91, 615
AcoI YGGCCR 1 cut(s) 637
AcsI RAATTY 1 cut(s) 589
AcuI CTGAAG 1 cut(s) 549
AcyI GRCGYC 3 cut(s) 30, 111, 147
AfaI GTAC 2 cut(s) 736, 770
AfiI CCNNNNNNNGG 3 cut(s) 204, 646, 662
AgsI TTSAA 2 cut(s) 193, 883
AleI CACNNNNGTG 1 cut(s) 296
Alw21I GWGCWC 2 cut(s) 159, 821
Alw26I GTCTC 2 cut(s) 395, 784
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 6 cut(s) 81, 126, 375, 465, 637, 944
ApeKI GCWGC 4 cut(s) 740, 755, 794, 797
ApoI RAATTY 1 cut(s) 589
AsiSI GCGATCGC 1 cut(s) 15
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 3 cut(s) 19, 32, 66
AsuC2I CCSGG 1 cut(s) 199
AsuHPI GGTGA 1 cut(s) 728
BanI GGYRCC 2 cut(s) 29, 260
BanII GRGCYC 2 cut(s) 159, 291
Bbv12I GWGCWC 2 cut(s) 159, 821
BbvI GCAGC 4 cut(s) 727, 767, 781, 809
BccI CCATC 5 cut(s) 232, 359, 530, 625, 914
BceAI ACGGC 2 cut(s) 241, 274
BcnI CCSGG 1 cut(s) 199
BcoDI GTCTC 2 cut(s) 395, 784
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 2 cut(s) 405, 795
BfoI RGCGCY 2 cut(s) 33, 67
BisI GCNGC 7 cut(s) 28, 67, 84, 741, 756, 795, 798
BlsI GCNGC 7 cut(s) 29, 68, 85, 742, 757, 796, 799
Bme1390I CCNGG 1 cut(s) 199
BmiI GGNNCC 2 cut(s) 31, 262
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 1 cut(s) 400
BpmI CTGGAG 2 cut(s) 86, 194
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 199
BsaHI GRCGYC 3 cut(s) 30, 111, 147
BsaJI CCNNGG 1 cut(s) 22
BsaWI WCCGGW 2 cut(s) 184, 430
Bsc4I CCNNNNNNNGG 3 cut(s) 204, 646, 662
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 712
Bse3DI GCAATG 1 cut(s) 838
BseDI CCNNGG 1 cut(s) 22
BseGI GGATG 5 cut(s) 39, 569, 754, 900, 925
BseLI CCNNNNNNNGG 3 cut(s) 204, 646, 662
BseMI GCAATG 1 cut(s) 838
BseMII CTCAG 1 cut(s) 906
BseNI ACTGG 1 cut(s) 712
BseRI GAGGAG 3 cut(s) 129, 333, 827
BseXI GCAGC 4 cut(s) 727, 767, 781, 809
BsgI GTGCAG 3 cut(s) 497, 687, 714
Bsh1236I CGCG 3 cut(s) 17, 78, 168
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 6 cut(s) 83, 128, 377, 467, 639, 946
BshNI GGYRCC 2 cut(s) 29, 260
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 2 cut(s) 159, 821
BsiSI CCGG 6 cut(s) 33, 59, 185, 198, 431, 640
BslFI GGGAC 2 cut(s) 662, 685
BslI CCNNNNNNNGG 3 cut(s) 204, 646, 662
BsmAI GTCTC 2 cut(s) 395, 784
BsmFI GGGAC 2 cut(s) 662, 685
BsnI GGCC 6 cut(s) 83, 128, 377, 467, 639, 946
Bsp1286I GDGCHC 3 cut(s) 159, 291, 821
Bsp143I GATC 3 cut(s) 12, 105, 580
Bsp19I CCATGG 1 cut(s) 22
Bsp68I TCGCGA 2 cut(s) 78, 168
BspACI CCGC 5 cut(s) 27, 67, 84, 91, 615
BspANI GGCC 6 cut(s) 83, 128, 377, 467, 639, 946
BspCNI CTCAG 1 cut(s) 907
BspFNI CGCG 3 cut(s) 17, 78, 168
BspLI GGNNCC 2 cut(s) 31, 262
BspMAI CTGCAG 2 cut(s) 409, 799
BspT107I GGYRCC 2 cut(s) 29, 260
BsrDI GCAATG 1 cut(s) 838
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 1 cut(s) 712
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 3 cut(s) 12, 105, 580
BssNI GRCGYC 3 cut(s) 30, 111, 147
BssT1I CCWWGG 1 cut(s) 22
Bst4CI ACNGT 3 cut(s) 232, 298, 559
BstACI GRCGYC 3 cut(s) 30, 111, 147
BstAPI GCANNNNNTGC 1 cut(s) 701
BstC8I GCNNGC 4 cut(s) 34, 71, 170, 456
BstDEI CTNAG 1 cut(s) 915
BstDSI CCRYGG 1 cut(s) 22
BstF5I GGATG 5 cut(s) 39, 569, 754, 900, 925
BstFNI CGCG 3 cut(s) 17, 78, 168
BstH2I RGCGCY 2 cut(s) 33, 67
BstHHI GCGC 3 cut(s) 19, 32, 66
BstKTI GATC 3 cut(s) 15, 108, 583
BstMAI GTCTC 2 cut(s) 395, 784
BstMBI GATC 3 cut(s) 12, 105, 580
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 9 cut(s) 33, 70, 75, 125, 464, 530, 701, 837, 854
BstSCI CCNGG 1 cut(s) 197
BstSFI CTRYAG 2 cut(s) 405, 795
BstUI CGCG 3 cut(s) 17, 78, 168
BstV1I GCAGC 4 cut(s) 727, 767, 781, 809
BsuRI GGCC 6 cut(s) 83, 128, 377, 467, 639, 946
BtgI CCRYGG 1 cut(s) 22
BtsCI GGATG 5 cut(s) 39, 569, 754, 900, 925
BtsI GCAGTG 1 cut(s) 94
BtsIMutI CAGTG 2 cut(s) 94, 705
BtuMI TCGCGA 2 cut(s) 78, 168
Cac8I GCNNGC 4 cut(s) 34, 71, 170, 456
CaiI CAGNNNCTG 1 cut(s) 701
CfoI GCGC 3 cut(s) 19, 32, 66
Cfr10I RCCGGY 1 cut(s) 32
CseI GACGC 1 cut(s) 100
Csp6I GTAC 2 cut(s) 735, 769
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviAII CATG 2 cut(s) 23, 331
CviQI GTAC 2 cut(s) 735, 769
DdeI CTNAG 1 cut(s) 915
DinI GGCGCC 1 cut(s) 31
DpnI GATC 3 cut(s) 14, 107, 582
DpnII GATC 3 cut(s) 12, 105, 580
EaeI YGGCCR 1 cut(s) 637
EciI GGCGGA 2 cut(s) 80, 630
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 22
Eco147I AGGCCT 1 cut(s) 377
Eco24I GRGCYC 2 cut(s) 159, 291
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 549
EcoICRI GAGCTC 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 22
EcoT38I GRGCYC 2 cut(s) 159, 291
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 22
FaeI CATG 2 cut(s) 26, 334
FaqI GGGAC 2 cut(s) 662, 685
FatI CATG 2 cut(s) 22, 330
FauNDI CATATG 1 cut(s) 676
Fnu4HI GCNGC 7 cut(s) 28, 67, 84, 741, 756, 795, 798
FokI GGATG 5 cut(s) 26, 576, 761, 907, 932
FriOI GRGCYC 2 cut(s) 159, 291
Fsp4HI GCNGC 7 cut(s) 28, 67, 84, 741, 756, 795, 798
FspBI CTAG 1 cut(s) 384
GlaI GCGC 3 cut(s) 18, 31, 65
GluI GCNGC 7 cut(s) 28, 67, 84, 741, 756, 795, 798
GsuI CTGGAG 2 cut(s) 86, 194
HaeII RGCGCY 2 cut(s) 33, 67
HaeIII GGCC 6 cut(s) 83, 128, 377, 467, 639, 946
HapII CCGG 6 cut(s) 33, 59, 185, 198, 431, 640
HgaI GACGC 1 cut(s) 100
HhaI GCGC 3 cut(s) 19, 32, 66
Hin1I GRCGYC 3 cut(s) 30, 111, 147
Hin1II CATG 2 cut(s) 26, 334
Hin6I GCGC 3 cut(s) 17, 30, 64
HinP1I GCGC 3 cut(s) 17, 30, 64
HincII GTYRAC 2 cut(s) 216, 625
HindII GTYRAC 2 cut(s) 216, 625
HindIII AAGCTT 1 cut(s) 243
HpaI GTTAAC 1 cut(s) 625
HpaII CCGG 6 cut(s) 33, 59, 185, 198, 431, 640
HphI GGTGA 1 cut(s) 728
Hpy166II GTNNAC 2 cut(s) 216, 625
Hpy188I TCNGA 4 cut(s) 504, 731, 825, 952
Hpy188III TCNNGA 4 cut(s) 77, 103, 143, 167
Hpy8I GTNNAC 2 cut(s) 216, 625
Hpy99I CGWCG 4 cut(s) 116, 149, 152, 164
HpyAV CCTTC 3 cut(s) 196, 508, 957
HpyCH4III ACNGT 3 cut(s) 232, 298, 559
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 7 cut(s) 407, 478, 602, 668, 695, 797, 958
HpyF10VI GCNNNNNNNGC 9 cut(s) 33, 70, 75, 125, 464, 530, 701, 837, 854
HpyF3I CTNAG 1 cut(s) 915
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 3 cut(s) 30, 111, 147
Hsp92II CATG 2 cut(s) 26, 334
HspAI GCGC 3 cut(s) 17, 30, 64
KasI GGCGCC 1 cut(s) 29
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
KspAI GTTAAC 1 cut(s) 625
Kzo9I GATC 3 cut(s) 12, 105, 580
LmnI GCTCC 4 cut(s) 24, 61, 275, 368
Lsp1109I GCAGC 4 cut(s) 727, 767, 781, 809
LweI GCATC 1 cut(s) 400
MaeI CTAG 1 cut(s) 384
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 868
MalI GATC 3 cut(s) 14, 107, 582
MboI GATC 3 cut(s) 12, 105, 580
MhlI GDGCHC 3 cut(s) 159, 291, 821
MluCI AATT 2 cut(s) 498, 589
Mly113I GGCGCC 1 cut(s) 30
MmeI TCCRAC 1 cut(s) 541
MreI CGCCGGCG 1 cut(s) 32
MroNI GCCGGC 1 cut(s) 32
MroXI GAANNNNTTC 1 cut(s) 419
MseI TTAA 2 cut(s) 624, 786
MslI CAYNNNNRTG 1 cut(s) 296
MspA1I CMGCKG 1 cut(s) 740
MspI CCGG 6 cut(s) 33, 59, 185, 198, 431, 640
MspR9I CCNGG 1 cut(s) 199
MvnI CGCG 3 cut(s) 17, 78, 168
MwoI GCNNNNNNNGC 9 cut(s) 33, 70, 75, 125, 464, 530, 701, 837, 854
NaeI GCCGGC 1 cut(s) 34
NarI GGCGCC 1 cut(s) 30
NciI CCSGG 1 cut(s) 199
NcoI CCATGG 1 cut(s) 22
NdeI CATATG 1 cut(s) 676
NdeII GATC 3 cut(s) 12, 105, 580
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 2 cut(s) 26, 334
NlaIV GGNNCC 2 cut(s) 31, 262
NmuCI GTSAC 1 cut(s) 868
NruI TCGCGA 2 cut(s) 78, 168
OliI CACNNNNGTG 1 cut(s) 296
PceI AGGCCT 1 cut(s) 377
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 165
PdiI GCCGGC 1 cut(s) 34
PdmI GAANNNNTTC 1 cut(s) 419
PkrI GCNGC 7 cut(s) 29, 68, 85, 742, 757, 796, 799
Ple19I CGATCG 1 cut(s) 15
PluTI GGCGCC 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 159
PspN4I GGNNCC 2 cut(s) 31, 262
PstI CTGCAG 2 cut(s) 409, 799
PstNI CAGNNNCTG 1 cut(s) 701
PvuI CGATCG 1 cut(s) 15
PvuII CAGCTG 1 cut(s) 740
RgaI GCGATCGC 1 cut(s) 15
RruI TCGCGA 2 cut(s) 78, 168
RsaI GTAC 2 cut(s) 736, 770
RsaNI GTAC 2 cut(s) 735, 769
RseI CAYNNNNRTG 1 cut(s) 296
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 2 cut(s) 624, 786
SatI GCNGC 7 cut(s) 28, 67, 84, 741, 756, 795, 798
Sau3AI GATC 3 cut(s) 12, 105, 580
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 3 cut(s) 159, 291, 821
SfaAI GCGATCGC 1 cut(s) 15
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 2 cut(s) 405, 795
SfoI GGCGCC 1 cut(s) 31
SgfI GCGATCGC 1 cut(s) 15
SgrAI CRCCGGYG 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 2 cut(s) 498, 589
SseBI AGGCCT 1 cut(s) 377
SsiI CCGC 5 cut(s) 27, 67, 84, 91, 615
SspDI GGCGCC 1 cut(s) 29
SspI AATATT 1 cut(s) 181
SspMI CTAG 1 cut(s) 384
SstI GAGCTC 1 cut(s) 159
StuI AGGCCT 1 cut(s) 377
StyD4I CCNGG 1 cut(s) 197
StyI CCWWGG 1 cut(s) 22
TaaI ACNGT 3 cut(s) 232, 298, 559
TaiI ACGT 1 cut(s) 150
TaqI TCGA 4 cut(s) 114, 144, 608, 901
TasI AATT 2 cut(s) 498, 589
TatI WGTACW 2 cut(s) 734, 768
TauI GCSGC 3 cut(s) 30, 69, 86
Tru1I TTAA 2 cut(s) 624, 786
Tru9I TTAA 2 cut(s) 624, 786
TscAI CASTG 2 cut(s) 101, 712
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 4 cut(s) 740, 755, 794, 797
Tsp45I GTSAC 1 cut(s) 868
TspDTI ATGAA 1 cut(s) 412
TspRI CASTG 2 cut(s) 101, 712
XapI RAATTY 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 1 cut(s) 384
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.